<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">SS</journal-id><journal-title-group><journal-title>Surgical Science</journal-title></journal-title-group><issn pub-type="epub">2157-9407</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/ss.2014.58055</article-id><article-id pub-id-type="publisher-id">SS-48461</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>MEDICINE &amp; HEALTHCARE</subject></subj-group></article-categories><title-group><article-title>Treating Tropical Pyomyositis (Myositis Tropicans) Using Primary Closure and Vacuum Drainage</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Joseph</surname><given-names>Yorke</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Pius</surname><given-names>Agbenorku</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Joseph</surname><given-names>Akpaloo</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Charles</surname><given-names>Appiah</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Papa</surname><given-names>Kwesi Sansomir Fiifi-Yankson</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Michael</surname><given-names>Adinku</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Ewura</surname><given-names>Adjoa Ahenfoa Ben-Crentsil</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Dennis</surname><given-names>Afful-Yorke</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib></contrib-group><aff id="aff1"><addr-line>Department of Surgery, School of Medical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana</addr-line></aff><aff id="aff2"><addr-line>Directorate of Surgery, Komfo Anokye Teaching Hospital, Kumasi, Ghana</addr-line></aff><aff id="aff3"><addr-line>School of Medical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>pimagben@yahoo.com(PA)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>01</day><month>08</month><year>2014</year></pub-date><volume>05</volume><issue>08</issue><fpage>333</fpage><lpage>337</lpage><history><date date-type="received"><day>23</day>	<month>May</month>	<year>2014</year></date><date date-type="rev-recd"><day>20</day>	<month>June</month>	<year>2014</year>	</date><date date-type="accepted"><day>15</day>	<month>July</month>	<year>2014</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
	Introduction: Tropical pyomyositis refers to
bacterial infection that affects skeletal muscles causing single or multiple
abscesses filled with pus. Aim: The study seeks to find out the effectiveness
in the use of primary closure and vacuum drainage in the treatment of
pyomyositis. Materials and Methods: The study was conducted between March 1,
2007 and February 28, 2014 at the Komfo Anokye Teaching Hospital, Kumasi, Ghana
on patients with pyomyositis. Results: Seventy-seven patients comprising 58
(75.32%) males and 19 (24.68%) females were included in the study with their
ages ranging from 15 to 68 years. There was improvement in patient’s healing
outcome. Conclusion: Primary wound closure and vacuum drainage provides patients
with much satisfaction and better outcome as well and it is also effective. 
</p></abstract><kwd-group><kwd>Pyomyositis</kwd><kwd> Primary Closure</kwd><kwd> Negative Wound Pressure Therapy (NWPT)</kwd><kwd> Satisfaction</kwd><kwd> Cost</kwd><kwd> Outcome</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>Tropical pyomyositis refers to bacterial infection that affects skeletal muscles causing single or multiple abscesses filled with pus mainly caused by Staphylococcus aureus. This condition is quite common in the tropics and referred to as myositis tropicans, tropical skeletal muscle abscess and tropical myositis; in temperate countries it is referred to as non-tropical myositis, infectious myositis or spontaneous bacterial myositis [<xref ref-type="bibr" rid="scirp.48461-ref1">1</xref>] -[<xref ref-type="bibr" rid="scirp.48461-ref4">4</xref>] . Group B, C and G Streptococcus, pneumococcus, Haemophilus spp., and Gram negative bacilli also cause the condition less commonly compared to Staphylococcus aureus occurring in 90% in tropical and 75% in temperate region. Muscles mostly affected include abdominal, spinal and gluteal muscles, quadriceps, pectoralis major, serratus anterior, biceps, iliopsoas and gastrocnemius [<xref ref-type="bibr" rid="scirp.48461-ref5">5</xref>] [<xref ref-type="bibr" rid="scirp.48461-ref6">6</xref>] . Diagnostic tools such as magnetic resonance imaging, computed tomography and ultrasound are useful. Confirmatory diagnosis is by biopsy or aspiration of pus from affected muscle [<xref ref-type="bibr" rid="scirp.48461-ref7">7</xref>] . Several disadvantages such as pains experienced during regular dressing and delay healing time are associated with incision and drainage technique developed in the treatment of acute abscesses [<xref ref-type="bibr" rid="scirp.48461-ref8">8</xref>] . Later, a technique known as primary closure was also introduced which offers advantage such as preventing secondary infection which is associated with open drains [<xref ref-type="bibr" rid="scirp.48461-ref9">9</xref>] . In the current study, authors seek to find out various parameters in the incision and primary closure with vacuum drainage in the treatment of pyomyositis.</p></sec><sec id="s2"><title>2. Materials and Methods</title><sec id="s2_1"><title>2.1. Study Setting</title><p>Komfo Anokye Teaching Hospital (KATH), located in Kumasi, is the second-largest hospital in Ghana and the only tertiary health institution in the middle belt of the country with currently 1000 bed capacity and an annual hospital attendance of about 679,050 patients made up of both out- and in-patients (KATH records). It is the main referral hospital for the Ashanti, Brong-Ahafo, Northern, Upper East, and Upper West regions.</p></sec><sec id="s2_2"><title>2.2. Patient Management</title><p>The study was conducted between March 1, 2007 and February 28, 2014 at the Komfo Anokye Teaching Hospital, Kumasi, Ghana and included patients with pyomyositis who presented to the Accident and Emergency Centre and given their consent to be part of the study. Protocols followed are same as in study by Akpaloo et al., 2013 [<xref ref-type="bibr" rid="scirp.48461-ref10">10</xref>] . Averagely, the device was used for 10 days with length of hospitalization ranging from 5 to 9 days.</p></sec><sec id="s2_3"><title>2.3. Ethical Approval</title><p>Ethical clearance was obtained from the Kwame Nkrumah University of Science and Technology School of Medical Sciences/Komfo Anokye Teaching Hospital Committee on Human Research, Publication and Ethics, Kumasi.</p></sec></sec><sec id="s3"><title>3. Results</title><p>Seventy-seven subjects with pyomyositis were treated using incision, primary closure and drainage under negative pressure wound therapy (NPWT). Of the 77 subjects treated, 58 (75.32%) were males and 19 (24.68%) were females. Ages of patients ranged from 15 to 68 years (<xref ref-type="table" rid="table1">Table 1</xref>). The length of admission ranged from 5 to 9 days.</p><p>Muscles mostly affected included abdominal, spinal and gluteal muscles, quadriceps, pectoralis major, serratus anterior, biceps, iliopsoas and gastrocnemius (<xref ref-type="table" rid="table2">Table 2</xref>).</p><p>Satisfaction rate of wound healing was assessed by patients on a scale of 1 - 5: 1: bad; 2: fair; 3: satisfactory; 4: good, and 5: excellent as shown in <xref ref-type="table" rid="table3">Table 3</xref>.</p><p>Severe wound infection was seen in 3 (3.9%) patients hence therapy was discontinued for them. <xref ref-type="table" rid="table4">Table 4</xref> shows the complications during the treatment.</p><p>A sample of the patients treated is shown in <xref ref-type="fig" rid="fig1">Figure 1</xref>.</p></sec><sec id="s4"><title>4. Discussion</title><p>This method of incision, drainage, primary closure and the connection of the drainage tube to the negative pressure</p><fig-group id="fig1"><caption><title>Figure 1</title><p> A patient with pyomyositis who had undergone the NPWT using the VAC device. (a) Incision and drainage of pus; (b) Drainage tubes inserted, wound closed primarily; (c) Bandaged, suction connected; (d) 5 days post OP; (e) Healed wound</p></caption><fig id ="fig1_1"><label>(a)</label><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://file.scirp.org/Html/htmlimages\1-2300746x\6c6c16a7-85ea-454f-b43a-cc987154e3e7.png"/></fig><fig id ="fig1_2"><label>(b)</label><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://file.scirp.org/Html/htmlimages\1-2300746x\c2e2783a-8e25-45dd-9ca3-15958ff4563d.png"/></fig><fig id ="fig1_3"><label>(c)</label><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://file.scirp.org/Html/htmlimages\1-2300746x\11b1238b-73ca-4cd7-a12a-be48cd8018ef.png"/></fig><fig id ="fig1_4"><label>(d)</label><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://file.scirp.org/Html/htmlimages\1-2300746x\294ccdc1-e4c5-429e-a9d2-0356736127df.png"/></fig><fig id ="fig1_5"><label>(e)</label><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://file.scirp.org/Html/htmlimages\1-2300746x\5f24bc8b-2d6a-4a11-93b3-25c981693706.png"/></fig></fig-group><table-wrap id="table1"  position="float"><object-id pub-id-type="pii">Table 1</object-id><label>Table 1</label><caption><p>. Patients distribution in the study.</p></caption><table><thead><tr><th align="center" valign="middle"  rowspan="2"  >Age</th><th align="center" valign="middle"  colspan="2"  >Gender</th><th align="center" valign="middle"  rowspan="2"  >Immunocompromised</th><th align="center" valign="middle"  rowspan="2"  >Diabetic</th></tr></thead><tbody><tr><td align="center" valign="middle" >Male</td><td align="center" valign="middle" >Female</td></tr><tr><td align="center" valign="middle" >15 - 25</td><td align="center" valign="middle" >5</td><td align="center" valign="middle" >3</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >0</td></tr><tr><td align="center" valign="middle" >26 - 35</td><td align="center" valign="middle" >14</td><td align="center" valign="middle" >1</td><td align="center" valign="middle" >1</td><td align="center" valign="middle" >1</td></tr><tr><td align="center" valign="middle" >36 - 45</td><td align="center" valign="middle" >22</td><td align="center" valign="middle" >4</td><td align="center" valign="middle" >7</td><td align="center" valign="middle" >5</td></tr><tr><td align="center" valign="middle" >46 - 55</td><td align="center" valign="middle" >10</td><td align="center" valign="middle" >5</td><td align="center" valign="middle" >2</td><td align="center" valign="middle" >3</td></tr><tr><td align="center" valign="middle" >56 - 65</td><td align="center" valign="middle" >4</td><td align="center" valign="middle" >5</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >1</td></tr><tr><td align="center" valign="middle" >66 - 75</td><td align="center" valign="middle" >3</td><td align="center" valign="middle" >1</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >1</td></tr></tbody></table></table-wrap><table-wrap id="table2"  position="float"><object-id pub-id-type="pii">Table 2</object-id><label>Table 2</label><caption><p>. Location of the pyomyositis.</p></caption><table><thead><tr><th align="center" valign="middle" >Anterior abdominal wall muscle</th><th align="center" valign="middle" >Quadriceps muscle</th><th align="center" valign="middle" >Gluteal muscle</th><th align="center" valign="middle" >Gastrocnemius muscle</th><th align="center" valign="middle" >Biceps muscle</th><th align="center" valign="middle" >Spinal muscle</th><th align="center" valign="middle" >Pectoralis muscle</th></tr></thead><tbody><tr><td align="center" valign="middle" >5 (6.5%)</td><td align="center" valign="middle" >38 (49.3%)</td><td align="center" valign="middle" >11 (14.3%)</td><td align="center" valign="middle" >16 (20.8%)</td><td align="center" valign="middle" >4 (5.2%)</td><td align="center" valign="middle" >2 (2.6%)</td><td align="center" valign="middle" >1 (1.3%)</td></tr></tbody></table></table-wrap><table-wrap id="table3"  position="float"><object-id pub-id-type="pii">Table 3</object-id><label>Table 3</label><caption><p>. Satisfaction rate of wound healing.</p></caption><table><thead><tr><th align="center" valign="middle" >Bad</th><th align="center" valign="middle" >0 (0.0%)</th></tr></thead><tbody><tr><td align="center" valign="middle" >Fair</td><td align="center" valign="middle" >0 (0.0%)</td></tr><tr><td align="center" valign="middle" >Satisfactory</td><td align="center" valign="middle" >2 (4.4%)</td></tr><tr><td align="center" valign="middle" >Good</td><td align="center" valign="middle" >8 (8.9%)</td></tr><tr><td align="center" valign="middle" >Excellent</td><td align="center" valign="middle" >67 (86.7%)</td></tr></tbody></table></table-wrap><table-wrap id="table4"  position="float"><object-id pub-id-type="pii">Table 4</object-id><label>Table 4</label><caption><p>. Complications arising from the use of the NPWT device.</p></caption><table><thead><tr><th align="center" valign="middle" >Severe</th><th align="center" valign="middle" >3 (3.9%)</th></tr></thead><tbody><tr><td align="center" valign="middle" >Mild</td><td align="center" valign="middle" >2 (2.6%)</td></tr><tr><td align="center" valign="middle" >None</td><td align="center" valign="middle" >72 (93.5%)</td></tr></tbody></table></table-wrap><p>wound therapy (NPWT) device (Variant I) provides suitable and preferable cosmetic outcome unlike unsatisfactory cosmetic outcome from the implementation of the incision and wound gauze package only (Variant II). Reduction in the number of dressing changes for Variant I benefits both the nurse who takes care of the wound and the patient with the condition in terms of decrease time as well as the pain experienced during dressing is reduced producing a better outcome for the patient. The primary closure system provides better satisfaction compared to the traditional incision and gauze package technique. Better outcome as a result of patient satisfaction provides relief to patient [<xref ref-type="bibr" rid="scirp.48461-ref11">11</xref>] . Also as has been reported by some authors, the primary closure system with subsequent connection to the NWPT prevents the patient from acquiring any secondary infection. Improved recovery rate implies reduction in hospitalisation and hospital cost. Administration of antibiotics only in the treatment of some abscesses is sometimes inadequate, hence the need for incision and drainage [<xref ref-type="bibr" rid="scirp.48461-ref12">12</xref>] . Incision and drainage often seen as an out-patient procedure is usually carried out in management of abscess or boil to release pus. This procedure may require treatment of the area with antibiotics and aseptically making an incision to allow the pus to drain through the incision. However, because of the possibility of infection, the patient should be treated with antibiotics. The use of the primary closure has been recommended to be beneficial due to its cost effectiveness and not associated with further wound complication. Thus basically this method utilizes negative pressure that promotes wound granulation similar to that obtained from the use of VAC developed by Argenta and Morykwas [<xref ref-type="bibr" rid="scirp.48461-ref13">13</xref>] . The primary wound closure and drainage has been reported to promote faster wound healing and reducing hospital stay. It is also reported to be a safe procedure. A study by Vosa et al. (2012) revealed the implementation of the primary wound closure system is safe and feasible and most importantly does not compromise on mortality thus, giving high survival rates for patients [<xref ref-type="bibr" rid="scirp.48461-ref12">12</xref>] .</p><p>In all the patients in our series we used this Variant I procedure only. We did no comparison with the Variant II in our series.</p></sec><sec id="s5"><title>5. Conclusion</title><p>The use of the primary wound closure and vacuum drainage in treating pyomyositis provides patients with much satisfaction, reduction in cost and hospital stay as well as more improved rate of healing and recovery than the former. It is thus more gratifying to the patient and the attendant health professional to use this method.</p></sec><sec id="s6"><title>Acknowledgements</title><p>The authors sincerely thank the numerous nurses and doctors who helped to manage these patients.</p></sec><sec id="s7"><title>Conflict of Interest</title><p>No conflict of interests is declared by any of the authors.</p></sec><sec id="s8"><title>NOTES@endMarkP#wang#_title:ep!!!</title><p></p><disp-formula id="scirp.48461-formula712"><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://file.scirp.org/Html/htmlimages\1-2300746x\b4a316c8-772c-460e-8375-0a52016b1cae.png"/></disp-formula><p><sup>*</sup>Corresponding author.</p><p></p></sec></body><back><ref-list><title>References</title><ref id="scirp.48461-ref1"><label>1</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>CHACHA</surname><given-names> P.B. </given-names></name>,<etal>et al</etal>. (<year>1970</year>)<article-title>MUSCLE ABSCESSES IN CHILDREN</article-title><source> &lt;I&gt;CLINICAL ORTHOPAEDICS&lt;/I&gt;</source><volume> 70</volume>,<fpage> 174</fpage>-<lpage>180</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.48461-ref2"><label>2</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>CHEIDOZI</surname><given-names> L.C. </given-names></name>,<etal>et al</etal>. (<year>1979</year>)<article-title>PYOMYOSITIS: REVIEW OF 205 CASES IN 112 PATIENTS</article-title><source> &lt;I&gt;THE AMERICAN JOURNAL OF SURGERY&lt;/I&gt;</source><volume> 137</volume>,<fpage> 255</fpage>-<lpage>259</lpage>.<pub-id pub-id-type="doi">HTTP://DX.DOI.ORG/10.1016/0002-9610(79)90158-2</pub-id></mixed-citation></ref><ref id="scirp.48461-ref3"><label>3</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>FOSTER</surname><given-names> W.D. </given-names></name>,<etal>et al</etal>. (<year>1965</year>)<article-title>THE BACTERIOLOGY OF TROPICAL PYOMYOSITIS IN UGANDA</article-title><source> &lt;I&gt;JOURNAL OF HYGIENE&lt;/I&gt;</source><volume> 63</volume>,<fpage> 517</fpage>-<lpage>524</lpage>.<pub-id pub-id-type="doi">HTTP://DX.DOI.ORG/10.1017/S002217240004540X</pub-id></mixed-citation></ref><ref id="scirp.48461-ref4"><label>4</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>LADIPO</surname><given-names> G.O. </given-names></name>,<name name-style="western"><surname> FAKUNLE</surname><given-names> Y.F. </given-names></name>,<etal>et al</etal>. (<year>1977</year>)<article-title>LADIPO, G.O. AND FAKUNLE, Y.F.  TROPICAL PYOMYOSITIS IN THE NIGERIAN SAVANNA</article-title><source> &lt;I&gt;TROPICAL AND GEOGRAPHICAL MEDICINE&lt;/I&gt;</source><volume> 29</volume>,<fpage> 223</fpage>-<lpage>228</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.48461-ref5"><label>5</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>SARUBBI</surname><given-names> F.A.</given-names></name>,<name name-style="western"><surname> GAFFORD</surname><given-names> G.D. </given-names></name>,<name name-style="western"><surname> BISHOP</surname><given-names> D.R. </given-names></name>,<etal>et al</etal>. (<year>1989</year>)<article-title>GRAM NEGATIVE BACTERIAL PYOMYOSITIS: UNIQUE CASE AND REVIEW</article-title><source> &lt;I&gt;REVIEWS OF INFECTIOUS DISEASES&lt;/I&gt;</source><volume> 11</volume>,<fpage> 789</fpage>-<lpage>792</lpage>.<pub-id pub-id-type="doi">HTTP://DX.DOI.ORG/10.1093/CLINIDS/11.5.789</pub-id></mixed-citation></ref><ref id="scirp.48461-ref6"><label>6</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>CHRISTIN</surname><given-names> L. </given-names></name>,<name name-style="western"><surname> SAROSI</surname><given-names> G.A. </given-names></name>,<etal>et al</etal>. (<year>1992</year>)<article-title>PYOMYOSITIS IN NORTH AMERICA: CASE REPORTS AND REVIEW</article-title><source> &lt;I&gt;CLINICAL INFECTIOUS DISEASES&lt;/I&gt;</source><volume> 15</volume>,<fpage> 668</fpage>-<lpage>677</lpage>.<pub-id pub-id-type="doi">HTTP://DX.DOI.ORG/10.1093/CLIND/15.4.668</pub-id></mixed-citation></ref><ref id="scirp.48461-ref7"><label>7</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>CHAUHAN</surname><given-names> S.</given-names></name>,<name name-style="western"><surname> JAIN</surname><given-names> S.</given-names></name>,<name name-style="western"><surname> VARMA</surname><given-names> S. </given-names></name>,<name name-style="western"><surname> CHAUHAN</surname><given-names> S.S. </given-names></name>,<etal>et al</etal>. (<year>2004</year>)<article-title>TROPICAL PYOMYOSITIS (MYOSITIS TROPICANS): CURRENT PERSPECTIVE</article-title><source> &lt;I&gt;POSTGRADUATE MEDICAL JOURNAL&lt;/I&gt;</source><volume> 80</volume>,<fpage> 267</fpage>-<lpage>270</lpage>.<pub-id pub-id-type="doi">HTTP://DX.DOI.ORG/10.1136/PGMJ.2003.009274</pub-id></mixed-citation></ref><ref id="scirp.48461-ref8"><label>8</label><mixed-citation publication-type="other" xlink:type="simple">BHISHAGRATNA, K.K. (1996) ENGLISH TRANSLATION OF SUSHRUTA SAMHITA (CA 600 B.C.). BOSE, CALCUTTA.</mixed-citation></ref><ref id="scirp.48461-ref9"><label>9</label><mixed-citation publication-type="other" xlink:type="simple">NGO, Q.D., LAM, V.W.T. AND DEANE, S.A. (2004) DROWNING IN DRAINAGE. THE LIVERPOOL HOSPITAL SURVIVAL GUIDE TO DRAINS AND TUBES. DEPARTMENT OF SURGERY, LIVERPOOL HOSPITAL, LIVERPOOL.</mixed-citation></ref><ref id="scirp.48461-ref10"><label>10</label><mixed-citation publication-type="other" xlink:type="simple">AKPALOO, J., YORKE, J., AGBENORKU, P. AND YENLI, E.M.T. (2013) MANAGING ACUTE WOUNDS WITH NEGATIVE PRESSURE SYSTEM IN A DEVELOPING COUNTRY. &lt;I&gt;ISRN PLASTIC SURGERY&lt;/I&gt;, 2013, ARTICLE ID: 796254. HTTP://DX.DOI.ORG/10.5402/2013/796254</mixed-citation></ref><ref id="scirp.48461-ref11"><label>11</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>SINGER</surname><given-names> A.J.</given-names></name>,<name name-style="western"><surname> THODE JR.</surname><given-names> H.C.</given-names></name>,<name name-style="western"><surname> CHALE</surname><given-names> S.</given-names></name>,<name name-style="western"><surname> TAIRA</surname><given-names> B.R. </given-names></name>,<name name-style="western"><surname> LEE</surname><given-names> C. </given-names></name>,<etal>et al</etal>. (<year>2011</year>)<article-title>PRIMARY CLOSURE OF CUTANEOUS ABSCESSES A SYSTEMATIC REVIEW</article-title><source> &lt;I&gt;AMERICAN JOURNAL OF EMERGENCY MEDICINE&lt;/I&gt;</source><volume> 29</volume>,<fpage> 361</fpage>-<lpage>366</lpage>.<pub-id pub-id-type="doi">HTTP://DX.DOI.ORG/10.1016/J.AJEM.2009.10.004</pub-id></mixed-citation></ref><ref id="scirp.48461-ref12"><label>12</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>VOSA</surname><given-names> R.J.</given-names></name>,<name name-style="western"><surname> YILMAZA</surname><given-names> A.</given-names></name>,<name name-style="western"><surname> SONKERA</surname><given-names> U.</given-names></name>,<name name-style="western"><surname> JOHANNES</surname><given-names> C.</given-names></name>,<name name-style="western"><surname> KLOPPENBURGA</surname><given-names> K. </given-names></name>,<name name-style="western"><surname> GEOFFREY</surname><given-names> T.L. </given-names></name>,<etal>et al</etal>. (<year>2012</year>)<article-title>PRIMARY CLOSURE USING REDON DRAINS VS VACUUM-ASSISTED CLOSURE IN POST-STERNOTOMY MEDIASTINITIS</article-title><source> &lt;I&gt;EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY&lt;/I&gt;</source><volume> 42</volume>,<fpage> E53</fpage>-<lpage>E57</lpage>.<pub-id pub-id-type="doi">HTTP://DX.DOI.ORG/10.1093/EJCTS/EZS404</pub-id></mixed-citation></ref><ref id="scirp.48461-ref13"><label>13</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>MORYKWAS</surname><given-names> M.J.</given-names></name>,<name name-style="western"><surname> ARGENTA</surname><given-names> L.C.</given-names></name>,<name name-style="western"><surname> SHELTON-BROWN</surname><given-names> E.I. </given-names></name>,<name name-style="western"><surname> MCGUIRT</surname><given-names> W. </given-names></name>,<etal>et al</etal>. (<year>1997</year>)<article-title>VACUUM-ASSISTED CLOSURE: A NEW METHOD FOR WOUND CONTROL AND TREATMENT: ANIMAL STUDIES AND BASIC FOUNDATION</article-title><source> &lt;I&gt;ANNALS OF PLASTIC SURGERY&lt;/I&gt;</source><volume> 38</volume>,<fpage> 553</fpage>-<lpage>562</lpage>.<pub-id pub-id-type="doi">HTTP://DX.DOI.ORG/10.1097/00000637-199706000-00001</pub-id></mixed-citation></ref></ref-list></back></article>