<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">NR</journal-id><journal-title-group><journal-title>Natural Resources</journal-title></journal-title-group><issn pub-type="epub">2158-706X</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/nr.2014.58034</article-id><article-id pub-id-type="publisher-id">NR-47012</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>EARTH &amp; ENVIRONMENTAL SCIENCES</subject></subj-group></article-categories><title-group><article-title>Physical and Quality Characteristics of Jupi Pineapple Fruits on Macronutrient and Boron Deficiency</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Maria</surname><given-names>José Mota Ramos</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Leandro</surname><given-names>Glaydson da Rocha Pinho</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib></contrib-group><aff id="aff2"><addr-line>Federal Institute of Education, Espírito Santo, Campus Itapina, Brazil</addr-line></aff><aff id="aff1"><addr-line>Research Regional Center and Tecnology Transfer of EMPAER/MT, Várzea Grande, Brazil</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>majumota@ig.com.br(MJMR)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>19</day><month>06</month><year>2014</year></pub-date><volume>05</volume><issue>08</issue><fpage>359</fpage><lpage>366</lpage><history><date date-type="received"><day>25</day>	<month>April</month>	<year>2014</year></date><date date-type="rev-recd"><day>26</day>	<month>May</month>	<year>2014</year>	</date><date date-type="accepted"><day>7</day>	<month>June</month>	<year>2014</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
	The deficiencies of mineral elements, change the
development of plants in a broad sense, and therefore may alter the growth and
fruit quality. The objective of this work was to evaluate the influence of macronutrient and boron
deficiencies on morphological characteristics of “Jupi” pineapple fruits. The treatments complete, -N, -P,
-K, -Ca, -Mg, -S and -B were applied as nutrient solutions in plastic pots with
14 kg of purified beach sand and one pineapple plant as the experimental unit.
The trial was set up a randomized complete blocks design with six replicate.
Nitrogen deficiency reduced fruit mass with and without crown, fruit length and
diameter and peduncle diameter, increased TA, TSS and vitamin C, reduced
TSS/TA, pH, pulp coloration and sensory acceptance of the fruits; phosphorus
deficiency reduced fruit mass with and without crown, fruit and crown length,
potassium deficiency reduced fruit mass with and without crown, length and diameter
peduncle and calcium deficiency reduced peduncle diameter. Peel thickness was
not affected by any deficiency. Nitrogen is the nutrient that most influenced
the fruits quality of pineapple “Jupi”. 
</p></abstract><kwd-group><kwd>Fruit Quality</kwd><kwd> Nutrient Solution</kwd><kwd> Fruit Mass</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>The participation of Brazil in the external market of pineapple is small, despite being a typical fruit of tropical and subtropical regions, and the country sustain the second largest world production [<xref ref-type="bibr" rid="scirp.47012-ref1">1</xref>] . However, in order to gain competitiveness in foreign markets it requires the provision of fruit of excellent quality. The cultivars planted in Brazil are the P&#233;rola and Smooth Cayenne. Jupi is resembles the “P&#233;rola”, which differs by cylindrical format of the fruit. The cylindrical form of the fruits gives that cultivar greater preference as industrial raw material for processing slices in syrup, for increased yield.</p><p>Climatic conditions, stages of ripening, varietal differences, mineral nutrition of plants, among other factors, influence sharply in physical characteristics and physical-chemical properties of pineapple.</p><p>The effects of nutritional deficiencies in size and quality of pineapple fruit are very variable and depend on mineral nutrient in question and the time when the deficiency symptoms appear. The fruit quality is in part attributed to physical characteristics responsible for external appearance, among which stand out the fruit’s size and shape and coloration of the shell, characteristics that constitute the first factor of acceptability of fruits by consumers [<xref ref-type="bibr" rid="scirp.47012-ref2">2</xref>] . The external appearance is the agent of quality of greater influence in the purchase of a product by the consumer, by reason of the association of the appearance of the fruit with edible quality.</p><p>Nitrogen typically increases the size and the weight of the fruit of pineapple [<xref ref-type="bibr" rid="scirp.47012-ref3">3</xref>] -[<xref ref-type="bibr" rid="scirp.47012-ref5">5</xref>] reported increased fresh fruit with and without crown with increasing doses of NPK. It was also noticed by [<xref ref-type="bibr" rid="scirp.47012-ref3">3</xref>] the influence of potassium on the diameter of the fruit. The importance of potash on fruit quality is based on its role as promoter of the synthesis of photosynthates and their transport to fruits, grains, tubers and plant storage organs, increasing the conversion of starch, protein, vitamins, oils, and others [<xref ref-type="bibr" rid="scirp.47012-ref6">6</xref>] .</p><p>The TSS/TA is one of the best ways to evaluate the flavor, being more representative than the isolated measurement of sugar or acidity, providing good idea of balance between these two components [<xref ref-type="bibr" rid="scirp.47012-ref7">7</xref>] .</p><p>One of the impediments to the growth of cultivated area of cv. Jupi has been the shortage of technical information, especially relating to nutrition and fertilization of culture, which can directly affect the quality of the fruit. The objective of this work was to evaluate the influence of macronutrients and boron deficiencies on the physical and quality characteristics of pineapple cv. Jupi.</p></sec><sec id="s2"><title>2. Materials and Methods</title><p>The experiment was set up in a greenhouse in Campos dos Goytacazes, RJ, on abril 15, 2004, using plantlets of “Jupi” pineapple obtained by tissue culture. It’s consisted of eight treatments: Complete, -N, -P, -K, -Ca, -Mg, -S and -B, distributed a randomized complete block design, with six replications. The experimental unit was represented by one plant placed into a plastic pot containing 14 kg of beach sand previously purified by soaking it with pure water until stable pH of 5.0 followed by a final wash with deionized water. The purification consisted of the soak of the sand with hydrochloric acid diluted in water in the proportion of 1:4 during about four hours, in plastic container of 60 liters and subsequent wash with pure water until that the pH was stabilized in close value of 5, when, then, it was made a final wash with deionized water.</p><p>Plantlets had an average size of 10 cm. From planting each pot received 500 ml of deionized water every other two days for 15 day, when new roots began to be emitted. From then each pot was supplied three times per week with 500 ml of the complete nutrient solution that presented the following composition, in mg∙L<sup>−1</sup>:  N(<inline-formula><inline-graphic xlink:href="http://file.scirp.org/Html/htmlimages\4-2000393x\b5172915-0f9b-438d-aa7e-4cfed417f3c6.png" xlink:type="simple"/></inline-formula>) =112; N(<inline-formula><inline-graphic xlink:href="http://file.scirp.org/Html/htmlimages\4-2000393x\05a3b7d9-de66-46af-95d3-00a88849bee7.png" xlink:type="simple"/></inline-formula>) = 3.5; P = 7.74; K = 156.4; Ca = 80; Mg = 24.3; S = 32;0; Cl = 1.77; Mn = 0.55; Zn = 0.13; Cu = 0.03; Mo = 0.06; B = 0.27; Fe = 2.23 with pH = 5.5. From 50 days after the beginning of the application of the complete solution or 65 days after planting, the -B treatment was started to be applied. The other treatments continued to get the complete solution up to 105 days after planting.</p><p>Thereafter the macronutrientes concentration of macronutrients was reduced to 10% of the complete solution and from 150 days after planting, the treatments -N, -P, -K, -Ca, -Mg and -S started to be fully applied. At 240 days after planting, the plants of the-N treatment presented very severe symptoms of deficiency. Therefore they received a nutrient solution with 10% of N of the complete solution for a period of four weeks in order, to reduce the risk of absence of fruit formation by these plants. The volumes of nutrient solution applied varied with plant age, from 500 ml up to 150 days after planting; to 700 ml from 150 days to 330 days after planting and finally 1 L until harvest, applied three times a week.</p><p>At eight months after planting flowering forcing was done by applying of 50 mL solution of Ethrel 0.1%, urea 2% and calcium hydroxide 0.035% into the central rosette of each plant [<xref ref-type="bibr" rid="scirp.47012-ref3">3</xref>] .</p><p>Fruits were harvest at the stage of 75% yellow rind, from 03/31/2005 to 05/02/2005. Fresh medium weight were determined from the fruit without crown through a digital scale, length of the fruit and peduncle with graduated ruler with precision of 1 mm and peduncle diameter with digital pachymeter . The firmness of the fruit was evaluated at 10 different spots, covering the median portion of the fruit, by means of a penetrometer; the juice content (%), the percentage ratio between the mass and the fruit juice. The pulp coloration was determined using the median cross-section of the fruit with the scales of notes adapted from [<xref ref-type="bibr" rid="scirp.47012-ref3">3</xref>] . The titratable Acidity (TA), total soluble solids (TSS), pH and vitamin C were determined in sample of juice extracted from fruit longitudinal diagonal section, which accounted for a quarter of each of their upper and lower halves. The TA was obtained by titration with sodium hydroxide 0.71 n, expressed in % of citric acid (g/100 ml of juice); the total soluble solids (Brix) were determined by refractometry using a Refractometer Abbe manual; the pH of the juice was determined by a digital ph meter and relationship TSS/TA was obtained by dividing the total soluble solids values for percentage of acidity. The vitamin C (mg of Ascorbic acid/100 ml of juice) was determined by titration of the juice with 2.6 solution-dichlorophenol-indophenol, sodium salt.</p><p>The concentration of N, P, K, Ca, Mg, S and B in the dry matter of “D” leaf were determined at 5, 7, 9 and 12 months after planting according to the methodology of [<xref ref-type="bibr" rid="scirp.47012-ref8">8</xref>] , modified by Monnerat S/D.</p><p>Data obtained were submitted to the variance analysis and averages of the treatments under deficiency were compared with those of the complete nutrient treatment by the test of Dunnet at 5%. The SAEG programme was used for statistical analysis.</p></sec><sec id="s3"><title>3. Results and Discussions</title><sec id="s3_1"><title>3.1. Fresh Weight of Fruits with and without Crown</title><p>The N, P and K deficiencies decreased the fruit weight with and without crown (<xref ref-type="table" rid="table1">Table 1</xref>). The N deficiency reduced fruit weight with crown by 57.59%, P by 26.78% and K by 22.8%. N, P and K concentrations at 9 months after planting, (inflorescence appearance time) was 59.5%, 59.7% and 78.6% lower than the one of the complete nutrient treatment, respectively and to 12 months was 68.9% and 60.6%, 94.5% lower (<xref ref-type="table" rid="table2">Table 2</xref>).</p><p>Working pineapple Jupi [<xref ref-type="bibr" rid="scirp.47012-ref5">5</xref>] observed significant quadratic response effect for fresh mass from fruit of CV. Jupi, with and without Crown depending on doses of NPK. Another author [<xref ref-type="bibr" rid="scirp.47012-ref9">9</xref>] also observed quadratic effect of increasing doses of N and K in increasing fresh mass pineapple MD-2. The influence of K in the productive characteristics of pineapple can be related mainly to his role in photosynthesis. The N is the nutrient responsible for increased productivity of pineapple, and your deficiencies changes negatively the growth, output and quality of fruit [<xref ref-type="bibr" rid="scirp.47012-ref3">3</xref>] [<xref ref-type="bibr" rid="scirp.47012-ref10">10</xref>] [<xref ref-type="bibr" rid="scirp.47012-ref11">11</xref>] .</p><p>Fruits of plants cultivated in complete nutrient solution reached average fruit weight of 1594 kg (<xref ref-type="table" rid="table1">Table 1</xref>). The average of fruit weight with crown was below those (1.78 kg) obtained by [<xref ref-type="bibr" rid="scirp.47012-ref12">12</xref>] . Second [<xref ref-type="bibr" rid="scirp.47012-ref13">13</xref>] , the weight consi-</p><table-wrap id="table1"  position="float"><object-id pub-id-type="pii">Table 1</object-id><label>Table 1</label><caption><p>. Effect of macronutrients and boron deficiencies on fruit fresh weight (FFW), with and without crown, length and diameter of “Jupi” pineapple fruit</p></caption><table><thead><tr><th align="center" valign="middle" >Treatments</th><th align="center" valign="middle" >FFW with crown</th><th align="center" valign="middle" >FFW without crown</th><th align="center" valign="middle" >Length</th><th align="center" valign="middle" >Diameter</th></tr></thead><tbody><tr><td align="center" valign="middle" ></td><td align="center" valign="middle"  colspan="2"  >Kg</td><td align="center" valign="middle"  colspan="2"  >Cm</td></tr><tr><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >1.594</td><td align="center" valign="middle" >1.452</td><td align="center" valign="middle" >18.1</td><td align="center" valign="middle" >11.8</td></tr><tr><td align="center" valign="middle" >-N</td><td align="center" valign="middle" >0.676-</td><td align="center" valign="middle" >0.532-</td><td align="center" valign="middle" >11.1-</td><td align="center" valign="middle" >9.2-</td></tr><tr><td align="center" valign="middle" >-P</td><td align="center" valign="middle" >1.167-</td><td align="center" valign="middle" >1.069-</td><td align="center" valign="middle" >12.0-</td><td align="center" valign="middle" >10.7 ns</td></tr><tr><td align="center" valign="middle" >-K</td><td align="center" valign="middle" >1.231-</td><td align="center" valign="middle" >1.062-</td><td align="center" valign="middle" >14.3 ns</td><td align="center" valign="middle" >10.7 ns</td></tr><tr><td align="center" valign="middle" >-Ca</td><td align="center" valign="middle" >1.613 ns</td><td align="center" valign="middle" >1.482 ns</td><td align="center" valign="middle" >12.7 ns</td><td align="center" valign="middle" >10.8 ns</td></tr><tr><td align="center" valign="middle" >-Mg</td><td align="center" valign="middle" >1.538 ns</td><td align="center" valign="middle" >1.404 ns</td><td align="center" valign="middle" >13.5 ns</td><td align="center" valign="middle" >10.7 ns</td></tr><tr><td align="center" valign="middle" >-S</td><td align="center" valign="middle" >1.496 ns</td><td align="center" valign="middle" >1.333 ns</td><td align="center" valign="middle" >15.2 ns</td><td align="center" valign="middle" >12.0 ns</td></tr><tr><td align="center" valign="middle" >-B</td><td align="center" valign="middle" >1.744 ns</td><td align="center" valign="middle" >1.580 ns</td><td align="center" valign="middle" >12.3 ns</td><td align="center" valign="middle" >10.7 ns</td></tr><tr><td align="center" valign="middle" >CV%</td><td align="center" valign="middle" >12.8</td><td align="center" valign="middle" >14.1</td><td align="center" valign="middle" >1.5</td><td align="center" valign="middle" >5.0</td></tr></tbody></table></table-wrap><p>In each collumn, means followed by +, - or ns either are larger, smaller than the one of the complete treatment, according to Dunnett’s test at 5% level.</p><table-wrap id="table2"  position="float"><object-id pub-id-type="pii">Table 2</object-id><label>Table 2</label><caption><p>. Leaf nutrient concentration in dry matter of “D” leaf, in complete and deficient treatment, in four times sampling</p></caption><table><thead><tr><th align="center" valign="middle"  rowspan="3"  >Nutrients</th><th align="center" valign="middle"  rowspan="3"  >Treatments</th><th align="center" valign="middle"  colspan="4"  >Times</th><th align="center" valign="middle"  rowspan="3"  >CV (%)</th></tr></thead><tbody><tr><td align="center" valign="middle"  colspan="4"  >Months after planting</td></tr><tr><td align="center" valign="middle" >Five</td><td align="center" valign="middle" >Seven</td><td align="center" valign="middle" >Nine</td><td align="center" valign="middle" >Ten</td></tr><tr><td align="center" valign="middle" >Nitrogen</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >(g∙kg<sup>−1</sup>)</td><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >16.3</td><td align="center" valign="middle" >16.9</td><td align="center" valign="middle" >18.6</td><td align="center" valign="middle" >17.4</td><td align="center" valign="middle" >6.10</td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >-N</td><td align="center" valign="middle" >9.22-</td><td align="center" valign="middle" >6.97-</td><td align="center" valign="middle" >7.58-</td><td align="center" valign="middle" >6.86-</td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Phosphorus</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >(g∙kg<sup>−1</sup>)</td><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >1.68</td><td align="center" valign="middle" >1.62</td><td align="center" valign="middle" >1.39</td><td align="center" valign="middle" >1.03</td><td align="center" valign="middle" >10.0</td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >-P</td><td align="center" valign="middle" >1.31-</td><td align="center" valign="middle" >0.97-</td><td align="center" valign="middle" >0.56-</td><td align="center" valign="middle" >0.32-</td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Potassium</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >(g∙kg<sup>−1</sup>)</td><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >27.7</td><td align="center" valign="middle" >25.5</td><td align="center" valign="middle" >28.5</td><td align="center" valign="middle" >27.25</td><td align="center" valign="middle" >8.9</td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >-K</td><td align="center" valign="middle" >18.3-</td><td align="center" valign="middle" >14.0-</td><td align="center" valign="middle" >6.10-</td><td align="center" valign="middle" >1.50-</td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Calcium</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >(g∙kg<sup>−1</sup>)</td><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >4.33</td><td align="center" valign="middle" >4.96</td><td align="center" valign="middle" >5.61</td><td align="center" valign="middle" >10.38</td><td align="center" valign="middle" >10.6</td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >-Ca</td><td align="center" valign="middle" >2.61-</td><td align="center" valign="middle" >1.43-</td><td align="center" valign="middle" >1.34-</td><td align="center" valign="middle" >1.67-</td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Magnesium</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >(g∙kg<sup>−1</sup>)</td><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >1.84</td><td align="center" valign="middle" >2.41</td><td align="center" valign="middle" >2.34</td><td align="center" valign="middle" >3.42</td><td align="center" valign="middle" >17.0</td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >-Mg</td><td align="center" valign="middle" >1.11-</td><td align="center" valign="middle" >0.98-</td><td align="center" valign="middle" >0.47-</td><td align="center" valign="middle" >0.57-</td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Sulphur</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >(g∙kg<sup>−1</sup>)</td><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >1.34</td><td align="center" valign="middle" >1.37</td><td align="center" valign="middle" >1.28</td><td align="center" valign="middle" >1.59</td><td align="center" valign="middle" >12.1</td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >-S</td><td align="center" valign="middle" >1.09-</td><td align="center" valign="middle" >1.05-</td><td align="center" valign="middle" >0.65-</td><td align="center" valign="middle" >0.37-</td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Boron</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >(mg∙kg<sup>−1</sup>)</td><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >23.9</td><td align="center" valign="middle" >23.2</td><td align="center" valign="middle" >33.2</td><td align="center" valign="middle" >44.4</td><td align="center" valign="middle" >6.5</td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >-B</td><td align="center" valign="middle" >12.6-</td><td align="center" valign="middle" >9.74-</td><td align="center" valign="middle" >7.20-</td><td align="center" valign="middle" >4.50-</td><td align="center" valign="middle" ></td></tr></tbody></table></table-wrap><p>In each column and each nutrient, means followed by - are significantly smaller than the complete treatment according to Dunnett’s test at 5% level.</p><p>dered great for pineapples of cv. Perola is between 1.0 - 1.4 kg; According to the classification by weight [<xref ref-type="bibr" rid="scirp.47012-ref14">14</xref>] , the fruit from the plant that received the complete solution and deficient in K (<xref ref-type="table" rid="table1">Table 1</xref>) would be covered in class 3 and 2, respectively and from plants deficient in N and P (<xref ref-type="table" rid="table1">Table 1</xref>) in class 1 reaching lowest value on the market. The difference in weight between fruit provides an additional significant financial gain for the producer, when pineapple is marketed as in natura.</p></sec><sec id="s3_2"><title>3.2. Fruit Length and Diameter</title><p>The fruit length was reduced by N and P deficiencies and the diameter by N deficiency (<xref ref-type="table" rid="table1">Table 1</xref>). The N deficiency reduced the length in 38.7% and the P in 33.7%; the fruit diameter was reduced by the N deficiency in 22%.</p><p>The fruit diameter of 11.8 cm (<xref ref-type="table" rid="table1">Table 1</xref>) was similar to that found by [<xref ref-type="bibr" rid="scirp.47012-ref13">13</xref>] for the “P&#233;rola”, it was 11.2 - 11.3 cm, but the length of 18.1 cm was slightly above found by this author.</p><p>The average of the fruit length and diameter, in complete treatment were similar to that found by [<xref ref-type="bibr" rid="scirp.47012-ref15">15</xref>] in their work with the cv. P&#233;rola whose length varied from 14 to 19.6 cm, and diameter of 10.31 cm to 11.05 cm, however, the length was above the value of 15.7 cm for cv. Smooth Cayenne and 16.1 cm from cv. P&#233;rola [<xref ref-type="bibr" rid="scirp.47012-ref16">16</xref>] .</p><p>Reducing the fruit weight caused by deficiencies resulted mainly from the reduction in the fruit length. In the N case referred to was, also, the smaller fruit diameter, reason why this was the deficiency that caused further reduction of fruit weight.</p></sec><sec id="s3_3"><title>3.3. Crown Length and Weight</title><p>P deficiency reduced the crown length but the weight was not affect by the deficiencies studied (<xref ref-type="table" rid="table3">Table 3</xref>). The crown length accepted quality standards varies according to the fruits class for export [<xref ref-type="bibr" rid="scirp.47012-ref17">17</xref>] .</p><p>In pineapple “Queen Victoria” was also found a decrease in fruit crown weight with the reduction in the levels of N [<xref ref-type="bibr" rid="scirp.47012-ref18">18</xref>] .</p><p>The fruit crown length from the complete solution is below 1.5 times the fruit length as [<xref ref-type="bibr" rid="scirp.47012-ref17">17</xref>] . Fruits in nitrogen deficient plant presented the crown within the medium considered patterns to “P&#233;rola” of 6.96% of the fruit weight, as [<xref ref-type="bibr" rid="scirp.47012-ref19">19</xref>] . It’s probably that larger values for the crown length have been obtained by applying the complete solution containing nitrogen until the fruit harvest.</p></sec><sec id="s3_4"><title>3.4. Peduncle Length and Diameter</title><p>The peduncle diameter was reduced by the shortcomings of N and K in 16.7% and 14.3% respectively and length by K and Ca deficiency in 17.5% and 15.1% respectively (<xref ref-type="table" rid="table3">Table 3</xref>).</p><p>Peduncle with smaller diameter may contribute to increase pineapple fruit drop what usually affect its quality and increases its sunburn susceptibility.</p></sec><sec id="s3_5"><title>3.5. Fruit Peel Thickness</title><p>There was not influence of nutritional deficiency in the peel thickness (<xref ref-type="table" rid="table3">Table 3</xref>).</p><p>It is likely that there has been a selective allocation of photosynthates in the bark as evolutionary strategy aiming to guarantee reproduction in environments poor in nutrients.</p></sec><sec id="s3_6"><title>3.6. TSS (Brix), TA e TSS/TA</title><p>The N deficiency increased TA in 85%, in the TSS in 11.2% and relationship TSS/TA. The S deficiency only increased the TSS in 12.5% (<xref ref-type="table" rid="table4">Table 4</xref>). At the time of fruit ripening the N concentration was 60.6% less than the full treatment (<xref ref-type="table" rid="table2">Table 2</xref>).</p><p>It is likely that the increase of TSS in the fruits, the treatment either by reducing the -S synthesis of sulfurous amino acids causing reduction of protein synthesis and accumulation of soluble carbohydrates. Plants grown under deficiency of N may have reduced production of amino acids and consequently protein providing greater amount of photosynthates to be used in the accumulation of other organic acids mainly in the synthesis of secondary metabolism compounds such as organic tricarboxylic acid cycle [<xref ref-type="bibr" rid="scirp.47012-ref20">20</xref>] .</p><p>For brix and acidity of the fruit, the responses to N were observed by [<xref ref-type="bibr" rid="scirp.47012-ref13">13</xref>] [<xref ref-type="bibr" rid="scirp.47012-ref21">21</xref>] who found a negative correlation for N. Discordant result was found by [<xref ref-type="bibr" rid="scirp.47012-ref8">8</xref>] who reported increased levels of brix and acidity of fruits of cv. Jupi</p><table-wrap id="table3"  position="float"><object-id pub-id-type="pii">Table 3</object-id><label>Table 3</label><caption><p>. Effect of macronutrients and boron deficiencies on crown length and weight peduncle length and diameter, and peel thickness of Jupi pineapple fruits</p></caption><table><thead><tr><th align="center" valign="middle" >Treatments</th><th align="center" valign="middle"  colspan="2"  >Crown</th><th align="center" valign="middle"  colspan="3"  >Peduncle</th><th align="center" valign="middle" >Peel thickness</th></tr></thead><tbody><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >Length</td><td align="center" valign="middle" >Weight</td><td align="center" valign="middle"  colspan="2"  >Length</td><td align="center" valign="middle" >Diameter</td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >Cm</td><td align="center" valign="middle" >Kg</td><td align="center" valign="middle"  colspan="3"  >Cm</td><td align="center" valign="middle" >Mm</td></tr><tr><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >24.6</td><td align="center" valign="middle" >0.142</td><td align="center" valign="middle" >38.4</td><td align="center" valign="middle"  colspan="2"  >2.09</td><td align="center" valign="middle" >2.29 ns</td></tr><tr><td align="center" valign="middle" >-N</td><td align="center" valign="middle" >22.1 ns</td><td align="center" valign="middle" >0.144 ns</td><td align="center" valign="middle" >36.8 ns</td><td align="center" valign="middle"  colspan="2"  >1.74-</td><td align="center" valign="middle" >2.19 ns</td></tr><tr><td align="center" valign="middle" >-P</td><td align="center" valign="middle" >18.3-</td><td align="center" valign="middle" >0.098 ns</td><td align="center" valign="middle" >34.5 ns</td><td align="center" valign="middle"  colspan="2"  >2.09 ns</td><td align="center" valign="middle" >2.50 ns</td></tr><tr><td align="center" valign="middle" >-K</td><td align="center" valign="middle" >27.4 ns</td><td align="center" valign="middle" >0.169 ns</td><td align="center" valign="middle" >31.7-</td><td align="center" valign="middle"  colspan="2"  >1.79-</td><td align="center" valign="middle" >2.24 ns</td></tr><tr><td align="center" valign="middle" >-Ca</td><td align="center" valign="middle" >21.7 ns</td><td align="center" valign="middle" >0.131 ns</td><td align="center" valign="middle" >32.6-</td><td align="center" valign="middle"  colspan="2"  >2.09 ns</td><td align="center" valign="middle" >2.31 ns</td></tr><tr><td align="center" valign="middle" >-Mg</td><td align="center" valign="middle" >24.8 ns</td><td align="center" valign="middle" >0.134 ns</td><td align="center" valign="middle" >38.1 ns</td><td align="center" valign="middle"  colspan="2"  >2.08 ns</td><td align="center" valign="middle" >2.26 ns</td></tr><tr><td align="center" valign="middle" >-S</td><td align="center" valign="middle" >25.1 ns</td><td align="center" valign="middle" >0.163 ns</td><td align="center" valign="middle" >34.6 ns</td><td align="center" valign="middle"  colspan="2"  >2.23 ns</td><td align="center" valign="middle" >2.38 ns</td></tr><tr><td align="center" valign="middle" >-B</td><td align="center" valign="middle" >24.6 ns</td><td align="center" valign="middle" >0.164 ns</td><td align="center" valign="middle" >35.2 ns</td><td align="center" valign="middle"  colspan="2"  >2.18 ns</td><td align="center" valign="middle" >2.33 ns</td></tr><tr><td align="center" valign="middle" >CV%</td><td align="center" valign="middle" >14.7</td><td align="center" valign="middle" >33.5</td><td align="center" valign="middle" >8.4</td><td align="center" valign="middle"  colspan="2"  >5.9</td><td align="center" valign="middle" >10.7</td></tr><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr></tbody></table></table-wrap><p>In each column, means followed by +, - or ns either are larger, smaller than the one of the complete treatment, according to Dunnett’s test at 5% level.</p><table-wrap id="table4"  position="float"><object-id pub-id-type="pii">Table 4</object-id><label>Table 4</label><caption><p>. Effect of macronutrients and boron deficiencies on TA, TSS, TSS/TA, Vitamin C, pH and pulp coloration (PC) of pineapple “Jupi” fruits</p></caption><table><thead><tr><th align="center" valign="middle" >Treatments</th><th align="center" valign="middle" >TA</th><th align="center" valign="middle" >TSS</th><th align="center" valign="middle" >TSS/TA</th><th align="center" valign="middle" >Vit. C</th><th align="center" valign="middle" >pH</th><th align="center" valign="middle" >PC<sup>*</sup></th></tr></thead><tbody><tr><td align="center" valign="middle" ></td><td align="center" valign="middle" >mg 100 g<sup>−1</sup></td><td align="center" valign="middle" ><sup>0</sup>Brix</td><td align="center" valign="middle" ></td><td align="center" valign="middle" >ascorbic acid (mg 100 g<sup>−1</sup>)</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >0.44</td><td align="center" valign="middle" >11.2</td><td align="center" valign="middle" >26.0</td><td align="center" valign="middle" >9.1</td><td align="center" valign="middle" >4.43</td><td align="center" valign="middle" >4.67</td></tr><tr><td align="center" valign="middle" >-N</td><td align="center" valign="middle" >0.81+</td><td align="center" valign="middle" >12.6+</td><td align="center" valign="middle" >16.9-</td><td align="center" valign="middle" >27.1+</td><td align="center" valign="middle" >3.60-</td><td align="center" valign="middle" >1.00-</td></tr><tr><td align="center" valign="middle" >-P</td><td align="center" valign="middle" >0.55 ns</td><td align="center" valign="middle" >11.7 ns</td><td align="center" valign="middle" >21.2 ns</td><td align="center" valign="middle" >7.8 ns</td><td align="center" valign="middle" >4.16 ns</td><td align="center" valign="middle" >4.00 ns</td></tr><tr><td align="center" valign="middle" >-K</td><td align="center" valign="middle" >0.48 ns</td><td align="center" valign="middle" >10.7 ns</td><td align="center" valign="middle" >21.0 ns</td><td align="center" valign="middle" >9.8 ns</td><td align="center" valign="middle" >4.13 ns</td><td align="center" valign="middle" >2.83-</td></tr><tr><td align="center" valign="middle" >-Ca</td><td align="center" valign="middle" >0.46 ns</td><td align="center" valign="middle" >11.7 ns</td><td align="center" valign="middle" >25.6 ns</td><td align="center" valign="middle" >8.8 ns</td><td align="center" valign="middle" >4.47 ns</td><td align="center" valign="middle" >4.33 ns</td></tr><tr><td align="center" valign="middle" >-Mg</td><td align="center" valign="middle" >0.43 ns</td><td align="center" valign="middle" >11.8 ns</td><td align="center" valign="middle" >28.3 ns</td><td align="center" valign="middle" >9.2 ns</td><td align="center" valign="middle" >4.58 ns</td><td align="center" valign="middle" >4.67 ns</td></tr><tr><td align="center" valign="middle" >-S</td><td align="center" valign="middle" >0.49 ns</td><td align="center" valign="middle" >12.8+</td><td align="center" valign="middle" >26.6 ns</td><td align="center" valign="middle" >13.9 ns</td><td align="center" valign="middle" >4.34 ns</td><td align="center" valign="middle" >4.50 ns</td></tr><tr><td align="center" valign="middle" >-B</td><td align="center" valign="middle" >0.50 ns</td><td align="center" valign="middle" >11.4 ns</td><td align="center" valign="middle" >24.6 ns</td><td align="center" valign="middle" >8.7 ns</td><td align="center" valign="middle" >4.42 ns</td><td align="center" valign="middle" >4.83 ns</td></tr><tr><td align="center" valign="middle" >CV (%)</td><td align="center" valign="middle" >25.4</td><td align="center" valign="middle" >6.1</td><td align="center" valign="middle" >21.6</td><td align="center" valign="middle" >34.9</td><td align="center" valign="middle" >5.2</td><td align="center" valign="middle" >15.7</td></tr></tbody></table></table-wrap><p>In each column, means followed by +, - or ns either are larger, smaller or don’t differ from the complete treatment, according to Dunnett’s test at 5% level. <sup>*</sup>Scale of notes: 1—pulp white, 2—pulp white with some yellow; 3—pulp more white than yellow; 4—pulp more yellow than white; 5—pulp yellow e 6-pulp yellow gold.</p><p>with increased dose of N.</p><p>The value of 0.44% TA in complete treatment , was similar to that achieved by [<xref ref-type="bibr" rid="scirp.47012-ref22">22</xref>] [<xref ref-type="bibr" rid="scirp.47012-ref23">23</xref>] of 0.4 and 0.41 to 0.65% of citric acid, respectively; and that is less than the value of the result of us TA 0.305 CV. Jupi [<xref ref-type="bibr" rid="scirp.47012-ref24">24</xref>] .</p><p>The TSS value of 11.2 (<xref ref-type="table" rid="table4">Table 4</xref>) was lower than that obtained by [<xref ref-type="bibr" rid="scirp.47012-ref24">24</xref>] of 13.6 for cv. Jupi. It is likely that during the period of harvest, March to May, the oscillation of temperature and luminosity contributed to TSS levels lower.</p><p>The decrease of the TSS/TA interface is associated to high acidity with a deficiency of this relationship is considered the best pineapple maturation index than the acidity and the Brix (TSS) isolated.</p><p>Working with cv. Jupi [<xref ref-type="bibr" rid="scirp.47012-ref5">5</xref>] noted reduction in relative brix/acidity with high doses of N, result not obtained in this work.</p></sec><sec id="s3_7"><title>3.7. Vit C and pH</title><p>The N deficiency increased the content of Vit. C in 3 times in relation to the content in the complete treatment and decreased pH in 18.7% (<xref ref-type="table" rid="table4">Table 4</xref>). The concentration of N was 59.0% and 60.0% less than the complete treatment on the flowering and fruit ripening stage, respectively (<xref ref-type="table" rid="table2">Table 2</xref>).</p><p>These increases were also observed for [<xref ref-type="bibr" rid="scirp.47012-ref10">10</xref>] [<xref ref-type="bibr" rid="scirp.47012-ref25">25</xref>] [<xref ref-type="bibr" rid="scirp.47012-ref26">26</xref>] . N deficiency reduces the synthesis of amino acids and thus of proteins, promoting greater availability of photosynthates to be used in the synthesis of secondary metabolism c4 mpounds, such as Ascorbic acid [<xref ref-type="bibr" rid="scirp.47012-ref20">20</xref>] .</p><p>The content of Vit. C, in the fruits grown in plants that have received the complete solution, was 9.1 mg∙kg<sup>−1</sup> lower than that obtained by [<xref ref-type="bibr" rid="scirp.47012-ref26">26</xref>] in the fruits of cv. P&#233;rola 17.65 mg of ascorbic acid per 100 g.</p><p>With the results obtained in this study it was observed that it is possible through the operation of fertilizing, produce fruits with higher levels of vitamin c.</p><p>The reduction in pH can be attributed to the accumulation of organic acids, whose values are represented by the TA and Vitamin C, due to the impairment of protein synthesis.</p></sec><sec id="s3_8"><title>3.8. Pulp Coloration</title><p>There was a pineapple pulp bleaching under deficiency of N and K (<xref ref-type="table" rid="table4">Table 4</xref>).</p><p>In the case of N deficiency, the clearer the fruit pulp also presented a higher value of TA and an underage relationship TSS/TA, compared to more colorful fruits. For nitrogen, pulp coloration could be indicative of nutritional deficiency.</p></sec><sec id="s3_9"><title>3.9. Sensory Analysis</title><p>Fruits grown in N and K deficient plants obtained organoleptic acceptance bills significantly lower than those of complete treatment (<xref ref-type="table" rid="table5">Table 5</xref>). There was no significant difference in the scores to the acceptance of the fruits of</p><table-wrap id="table5"  position="float"><object-id pub-id-type="pii">Table 5</object-id><label>Table 5</label><caption><p>. Effect of macronutrients and boron deficiencies in pulp approval test of “Jupi” pineapple fruit</p></caption><table><thead><tr><th align="center" valign="middle" >Treatments</th><th align="center" valign="middle" >Sensorial analyse (notes given)</th></tr></thead><tbody><tr><td align="center" valign="middle" >Complete</td><td align="center" valign="middle" >7.63</td></tr><tr><td align="center" valign="middle" >-N</td><td align="center" valign="middle" >5.60-</td></tr><tr><td align="center" valign="middle" >-P</td><td align="center" valign="middle" >7.33</td></tr><tr><td align="center" valign="middle" >-K</td><td align="center" valign="middle" >6.40-</td></tr><tr><td align="center" valign="middle" >-Ca</td><td align="center" valign="middle" >6.70</td></tr><tr><td align="center" valign="middle" >-Mg</td><td align="center" valign="middle" >6.93</td></tr><tr><td align="center" valign="middle" >-S</td><td align="center" valign="middle" >7.70</td></tr><tr><td align="center" valign="middle" >-B</td><td align="center" valign="middle" >7.47</td></tr><tr><td align="center" valign="middle" >Cv (%)</td><td align="center" valign="middle" >20</td></tr></tbody></table></table-wrap><p>In line, means followed by +, - or ns either are larger, smaller or don’t differ from the complete treatment, respectively, according to Dunnett’s test at 5% level. Test approval: each answer was correspond respectively at: 1 (dislike extremely), 2 (dislike a lot), 3 (dislike moderately), 4 (dislike lightly), 5 (indifferent), 6 (liked lightly), 7 (liked moderately), 8 (liked a lot) e 9 (liked extremely).</p><p>pineapple under the deficiencies of P, Ca, Mg, S, and B.</p><p>Some tasters found the pineapple pieces produced under N deficiency, watery, tasteless characteristic of pineapple, salt flavor, high acidity, fibrous, pale coloring and watery, tasteless K, little sweet, little juice. The excess acidity was the most cited characteristic, in the comments of assessors in relation to N deficiency.</p><p>It is likely that the higher acidity and the whitish coloration of the pulp have contributed to the notes attributed to the −N treatment (<xref ref-type="table" rid="table4">Table 4</xref>). Consumer preference is for fruits less acidic and more attractive in the external aspect.</p></sec></sec><sec id="s4"><title>4.Conclusions</title><p>-N deficiency reduced the fruit mass with crown at 57.6%, 38.7% in length and sensory acceptance of the fruits, but increased the titratable acidity in 85%; the total soluble solids and vitamin C;</p><p>-The fruit mass with crown was reduced by 26.8% P deficiency and the fruit length of in 33.7%;</p><p>-K deficiency reduced in 22.8% the fruit mass with crown, 17.5% the peduncle length, 14.36% the diameter and sensory acceptance of the fruits;</p><p>-The peel thickness was not affected by any deficiency;</p><p>-Nitrogen is the nutrient that most influenced the quality of the pineapple “Jupi”.</p></sec><sec id="s5"><title>Acknowledgements</title><p>We thank the FAPERJ for their financial support.</p></sec></body><back><ref-list><title>References</title><ref id="scirp.47012-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">FAOSTAT DATABASE HTTP://FAOSTAT.FAO.ORG/SITE/567/DEFAULT.ASPX</mixed-citation></ref><ref id="scirp.47012-ref2"><label>2</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>CARVALHO</surname><given-names> V.D. 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