<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">FNS</journal-id><journal-title-group><journal-title>Food and Nutrition Sciences</journal-title></journal-title-group><issn pub-type="epub">2157-944X</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/fns.2014.513133</article-id><article-id pub-id-type="publisher-id">FNS-47721</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>MEDICINE &amp; HEALTHCARE</subject><subject>BIOMEDICAL &amp; LIFE SCIENCES</subject><subject>CHEMISTRY &amp; MATERIALS SCIENCE</subject></subj-group></article-categories><title-group><article-title>Fermented Milk Products from Different Milk Types</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Olusola</surname><given-names>Ladokun</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Sarah</surname><given-names>Oni</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib></contrib-group><aff id="aff1"><addr-line>Department of Biochemistry, Lead City University, Ibadan, Nigeria</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>solaJP@gmail.com(OL)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>09</day><month>07</month><year>2014</year></pub-date><volume>05</volume><issue>13</issue><fpage>1228</fpage><lpage>1233</lpage><history><date date-type="received"><day>20</day>	<month>April</month>	<year>2014</year></date><date date-type="rev-recd"><day>29</day>	<month>May</month>	<year>2014</year>	</date><date date-type="accepted"><day>8</day>	<month>June</month>	<year>2014</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>Yogurt was produced from milk obtained from cow milk, goat milk, soymilk
and coconut milk by fermentation using starter cultures of <em>Lactobacillus bulgaricus</em> and <em>Lactobacillus acidophilus</em>.The results obtained showed that the initial pH of the fresh milk samples
were slightly acidic: cow milk (6.3), goat milk (6.2), soymilk (6.4) and coconut
milk (6.0). The pH results of the various fermented milk at 0 hour of production
were goat milk (5.24), cow milk (5.85), soymilk (5.73) and coconut milk (5.98),
but at 72 hours, all the milk samples tended to be more acidic due to the fermentation
and had lower pH values. All the fresh milk samples had the high moisture content
which ranged from 63.34% - 76.90%. Fat content ranged between 9.76% - 15.02%. Crude
protein ranged from 7.17% - 32.17% with goat milk having the highest protein level
of (32.17%). Ash content had the range of 0.52% - 0.96%. Goat milk had the highest
ash content value and coconut milk had the least value. Specific gravities of soymilk,
goat milk, cow milk and coconut milk were 1.018, 1.030, 1.016 and 1.01 g/ml respectively.
Taste, color, mouth feel and odor were acceptable at 0 hours of production but their
value depreciated with storage at room temperature. This study was able to establish
the close nutritional gap between cow milk, goat milk, soya and coconut milk yoghurt
preparations. The nutritional values obtained from the proximate analysis of the
milk samples were comparable. This clearly points to the fact that either of the
food can substitute for each other based on the values established from this study.</p></abstract><kwd-group><kwd>Yoghurt</kwd><kwd> Milk</kwd><kwd> &lt;i&gt;Lactobacillus bulgaricus&lt;/i&gt; and &lt;i&gt;Lactobacillus acidophilus&lt;/i&gt;</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>Milk is a white liquid produced by the mammary glands of mammals. It is the primary source of nutrition for young mammals before they are able to digest other types of food. Early-lactation milk contains colostrum, which carries the mother’s antibodies to the baby and can reduce the risk of many diseases in the baby [<xref ref-type="bibr" rid="scirp.47721-ref1">1</xref>] . The major chemical components of milk include water, fats, proteins, carbohydrates, minerals, organic acids, enzymes and vitamins [<xref ref-type="bibr" rid="scirp.47721-ref2">2</xref>] . Several legume-based milk and milk products have been developed in attempts to extend the supply of milk-like products, especially in areas where milk is in short supply. Since legumes are important sources of relatively inexpensive protein, introduction of imitation milk products from legumes may contribute to the alleviation of protein malnutrition [<xref ref-type="bibr" rid="scirp.47721-ref3">3</xref>] . Traditionally, yogurt is fermented whole milk and is believed to possess nutritional and therapeutic properties [<xref ref-type="bibr" rid="scirp.47721-ref4">4</xref>] [<xref ref-type="bibr" rid="scirp.47721-ref5">5</xref>] . Lactic acid fermentation of legume based milks has been used as one of the approaches to prolong the shelf life of the products, create variety, improve the nutritional value and as well enhance the acceptability of the product. Yogurt-like products have been prepared by some workers from soybean [<xref ref-type="bibr" rid="scirp.47721-ref6">6</xref>] , cowpeas, coconut and mug beans [<xref ref-type="bibr" rid="scirp.47721-ref3">3</xref>] .</p><p>This study is aimed at conducting a proximate analysis of the samples, to determine the yielding strength of each milk in yogurt production, taking pH and sensory evaluation as marker for their yields studying the possibility of using the milk derived from coconut, soybeans, goat and cow as a yogurt-like product</p></sec><sec id="s2"><title>2. Materials and Methodology</title><sec id="s2_1"><title>2.1. Collection of Milk Samples</title><p>Fresh Cow milk was obtained from the nomadic cattle herdsmen at Ijaiye, Southwestern Nigeria. The goat milk was obtained from Akesan farm house in Oyo town, Southwestern Nigeria. The collected samples were stored in sterile 2 liter containers. The samples after collection were kept in an ice-frozen container and immediately transported to the laboratory and kept in the refrigerator at 4˚C until it was ready for use. Soya beans and coconut were purchased locally from Orita-challenge market in Ibadan, and kept at ambient temperature prior to usage. The soya beans and coconut were subsequently taken for identification at the Agronomy department, University of Ibadan. They were identified as Glycine max and Cocos nucifera L. respectively.</p><sec id="s2_1_1"><title>2.1.1. Soymilk Preparation</title><p>Soya beans was sorted to remove stones and damaged, deformed seeds. 300 g of soya beans was soaked in 2 litres of warm water for 6 hours to remove the skin and to make it easier for grinding. The beans were drained, rinsed with potable water and grinded with about 500 ml of water in a eurosonic auto-clean blender. The resulting slurry was filtered through a muslin cloth and the extract obtained was boiled for 25 minutes. The milk was allowed to cool, and refrigerated [<xref ref-type="bibr" rid="scirp.47721-ref6">6</xref>] .</p></sec><sec id="s2_1_2"><title>2.1.2. Coconut Milk Preparation</title><p>Coconut milk was prepared by breaking the shells and taking the nuts by using a dull knife. The weight of the coconut was 306.3 g. The brown skin was removed from the nuts with a knife and the nuts were thoroughly washed. The nuts were blended with 200 ml warm water and allowed to stand for 20 minutes. The extract was passed through 0.18 mm sieve while the residue was discarded [<xref ref-type="bibr" rid="scirp.47721-ref7">7</xref>] . The milk obtained was eventually refrigerated.</p></sec><sec id="s2_1_3"><title>2.1.3. Starter Culture</title><p>Lactobacillus bulgaricus and Lactobacillus acidophilus were purchased locally from the market in Ibadan, Southwestern Nigeria.</p></sec></sec><sec id="s2_2"><title>2.2. Chemical and Proximate Analysis</title><p>The pH value was determined immediately after production, 24 hours and 72 hours respectively. The moisture content was determined by placing 10 g of the different samples in an oven maintained at 105˚C for 6 hours. Total ash was determined by heating in a muffle furnace at 630˚C for 3 hours. Protein content was determined through Kjedahl analysis using block digestion and steam distillation. Determination of ether extract was carried out using Soxhlet apparatus.</p></sec><sec id="s2_3"><title>2.3. Processing of Experimental Yogurts</title><p>Fresh goat milk, cow milk, soymilk and coconut milk samples were separately processed into yogurt (fermented milk using the starter culture). Fresh milk was preheated to about 45˚C and filtered. 15 g of sugar was added and mixed to each milk sample. Milk samples were pasteurized at 95˚C and held for 30 minutes, and then it was cooled to inoculation temperature of 45˚C in closed vessel and in a bath of cold water. 2.5 g of starter culture and 2 ml food flavour were added and mixed. This was incubated at 45˚C for 3 hours to coagulate [<xref ref-type="bibr" rid="scirp.47721-ref8">8</xref>] . After coagulation, it was stored in a bath of cold water overnight. Pasteurization is believed to modify milk protein so as to enhance proper viscosity and gelatinization of the product [<xref ref-type="bibr" rid="scirp.47721-ref4">4</xref>] . And this could account for the uniformity and smoothness in body texture in all products as observed in this study resulting to these sensory quality attributes (texture and mouth feel) not being significantly different.</p><p>The products were then ready for analysis and sensory evaluation. This procedure was done for all the four (4) milk samples and the control (Dano skimmed milk).</p><p>All the yogurt-like samples were subjected to sensory assessment by people that are familiar with the quality attributes of these yogurt products. Clean cups were provided for each sample; each panelist was requested to taste the samples one after the other and to indicate their degree of likeness or preference. The samples were evaluated for taste, sweetness, texture and odor. The panelist were asked to rate the samples for appearance, flavour, texture, aroma. The ratings were scored from a Hedonic scale ranging from 1.0 - 5.0.</p><p>Keys:</p><p>5.0 = Very Good.</p><p>4.9 - 4.0 = Good.</p><p>3.9 - 3.0 = Fair.</p><p>2.9 - 2.0 = Poor.</p><p>1.9 - 1.0 = Bad.</p><sec id="s2_3_1"><title>Statistical Analysis</title><p>The results obtained were subjected to analysis of variance (ANOVA). Mean comparison were carried out among the 4 yogurt-like products by Statistical Programs for Social Sciences (SPSS, 1992).</p></sec></sec></sec><sec id="s3"><title>3. Result and Discussion</title><p><xref ref-type="table" rid="table1">Table 1</xref> shows the proximate composition of the different milk samples. The ash content ranged between 0.5 and 0.96%. The ash content is a reflection of the mineral compositions of the milk samples and is quite comparable. This was an indication that all the samples investigated had micronutrients. However it is apparent that the value of goat milk (0.96%) was higher than the other milk samples [<xref ref-type="bibr" rid="scirp.47721-ref9">9</xref>] . The ash content which was highest in goat milk and lowest in coconut milk could be due to the salt lick activities done by the herbivores [<xref ref-type="bibr" rid="scirp.47721-ref10">10</xref>] . All the samples had high moisture contents which ranged between 63.34% - 76.90%. The moisture content of cow milk was the highest with 76.90% followed by that of goat milk, then coconut milk and soymilk. This could affect the stability and safety of food with respect to microbial growth and proliferation hence the products will require cold storage.</p><p>The moisture content of the milk samples studied was in conformity [<xref ref-type="bibr" rid="scirp.47721-ref11">11</xref>] . The significance of moisture content in milk is that, high moisture content implies high water activity which supports microbial growth consequently reducing the shelf life of the milk sample [<xref ref-type="bibr" rid="scirp.47721-ref12">12</xref>] . The pH value showed that soymilk was the least acidic of the milk samples and will be suitable for ulcer. Soymilk is regarded as a stimulant and tonic and it can be used in</p><table-wrap id="table1"  position="float"><object-id pub-id-type="pii">Table 1</object-id><label>Table 1</label><caption><p>. Proximate composition of the different milk samples</p></caption><table><thead><tr><th align="center" valign="middle" >Parameters</th><th align="center" valign="middle" >Cow Milk</th><th align="center" valign="middle" >Goat Milk</th><th align="center" valign="middle" >Soy Milk</th><th align="center" valign="middle" >Coconut Milk</th></tr></thead><tbody><tr><td align="center" valign="middle" >% Ash</td><td align="center" valign="middle" >0.71</td><td align="center" valign="middle" >0.96</td><td align="center" valign="middle" >0.57</td><td align="center" valign="middle" >0.52</td></tr><tr><td align="center" valign="middle" >% Moisture</td><td align="center" valign="middle" >76.90</td><td align="center" valign="middle" >73.00</td><td align="center" valign="middle" >63.34</td><td align="center" valign="middle" >65.00</td></tr><tr><td align="center" valign="middle" >pH Values</td><td align="center" valign="middle" >6.3</td><td align="center" valign="middle" >6.2</td><td align="center" valign="middle" >6.4</td><td align="center" valign="middle" >6.0</td></tr><tr><td align="center" valign="middle" >% Fat</td><td align="center" valign="middle" >9.76</td><td align="center" valign="middle" >14.84</td><td align="center" valign="middle" >14.44</td><td align="center" valign="middle" >15.02</td></tr><tr><td align="center" valign="middle" >% Crude Protein</td><td align="center" valign="middle" >27.97</td><td align="center" valign="middle" >32.17</td><td align="center" valign="middle" >12.59</td><td align="center" valign="middle" >7.17</td></tr><tr><td align="center" valign="middle" >Specific Gravity</td><td align="center" valign="middle" >1.018</td><td align="center" valign="middle" >1.030</td><td align="center" valign="middle" >1.016</td><td align="center" valign="middle" >1.010</td></tr></tbody></table></table-wrap><p>the treatment of indigestion, colic diarrhea and dysentery [<xref ref-type="bibr" rid="scirp.47721-ref13">13</xref>] . pH values of prepared yoghurts are near neutrality [<xref ref-type="bibr" rid="scirp.47721-ref14">14</xref>] [<xref ref-type="bibr" rid="scirp.47721-ref15">15</xref>] . The fat content of the milk samples ranged from 9.76% - 15.02%. The major contrast in the food proximate content was the values obtained for the fat contents. From previous studies, fat contents are usually higher in animal origin than plant origin. The findings in this study disagree with earlier studies because coconut milk was the richest in fat with 15.02%, followed by goat milk with 14.84%. Soymilk had a fat content of 14.44% and cow milk had the least value with 9.76% [<xref ref-type="bibr" rid="scirp.47721-ref16">16</xref>] . Fat content of food is the major contributor to the energy value of food. This implies that coconut milk and goat milk are more energy yielding than soya and cow milk. This is because of low risk of dietary cholesterol. Fats of animal origin like goat rather than plant usually contain some high saturated fatty acid implicated for raising blood cholesterol [<xref ref-type="bibr" rid="scirp.47721-ref17">17</xref>] . The presence of high levels of cholesterol in the bloodstream may form some fatty deposits in the artery which is a serious marker for arthrosclerosis and it can form plagues which can cause obstruction in the blood stream. These deposits may slow or block the flow of blood through the body and possibly causing heart attack or stroke. The total energy value of the milk is from the fat content hence, higher fat content is an indication of more total energy available. High fat content of coconut milk and soymilk further enhances their utilization for the extraction of edible oil thereby enhancing their nutrient densities as well as boosting their value as alternative feed supplement in human and livestock diets. The protein content of the milk samples ranged from 7.17% - 32.17%. Goat milk had the highest protein level of 32.17% followed by cow milk with 27.97% then soy milk and coconut milk had 12.59% and 7.17%. The protein content is in agreement with the work of Henry et al. [<xref ref-type="bibr" rid="scirp.47721-ref11">11</xref>] . Although, most authors recorded a very low value for the crude protein [<xref ref-type="bibr" rid="scirp.47721-ref11">11</xref>] , it is believed that this might be due to the fact that the milk samples that were analyzed by most of these authors are dry milk sample. The high protein content of this product showed that its consumption will help eliminate protein deficiencies that have become the bane of poor nations. Lactation could be responsible for the high protein content seen in the milk samples of goat and cow milk respectively. Soymilk also contain appreciable amount of protein sufficient for body growth and development. Milk protein is considered complete proteins because it contains all of the essential amino acids our bodies need. Plant proteins, found in soy beans and coconut lacked one or more of the essential amino acids. However, plant proteins can be combined in the diet to provide all of the essential amino acids. Marasmus and kwashiorkor, both life-threatening conditions, are the two most common forms of protein malnutrition. Some health conditions, such as illness, stress, pregnancy and breast-feeding in women, place an enormous demand on the body as it builds tissue or fights infection, and these conditions require an increase in protein consumption. For example, the digestive system breaks protein down into amino acids which may enhance tissue growth and repair to remedy whatever damage occur to body. Dietary proteins are powerful compounds that build and repair body tissues, from hair and fingernails to muscles. In addition to maintaining the body’s structure, proteins speed up chemical reactions in the body, serve as chemical messengers, fight infection, and transport oxygen from the lungs to the body’s tissues. Proteins are made of smaller units called amino acids. Specific gravity is basically done to determine the volume equivalent to 1 g of each milk samples so as to establish the volume corresponding to mass required when subjected to proximate analysis. The specific gravity for soymilk was 1.018 g/ml, goat milk had a specific gravity of 1.03 g/ml, cow milk had 1.016 g/ml and coconut milk had 1.01 g/ml.</p><p>Yogurt is a fermented milk product whose typical flavour (sour taste) is attributable to the production of lactic acids, acetaldehyde, acetic acid and diacetyl from carbohydrate by the fermenting organisms [<xref ref-type="bibr" rid="scirp.47721-ref18">18</xref>] .</p><p>The lactose (as added) probably influenced the sugar content of all the products. Furthermore, coagulation of milk became evident 4 hours following fermentation as it was seen that the pH of the milk products dropped which signified that they tend to be more acidic [<xref ref-type="bibr" rid="scirp.47721-ref7">7</xref>] [<xref ref-type="bibr" rid="scirp.47721-ref19">19</xref>] . The shelf life and the gradual decrease in the pH of fermenting milk samples (goat milk, cow milk, soy milk and coconut milk) during yogurt production over a 72 hours period is shown in <xref ref-type="table" rid="table2">Table 2</xref>.</p><p><xref ref-type="table" rid="table3">Table 3</xref> reveals the mean scores for acceptability of all the milk samples.</p><p>The skimmed milk served as the control. It was noticed that the shell life of the milk yogurt samples derived from plant origin (soy and coconut) was less than 72 hours. The shell life reported for the cow and goat milk was around 27 - 30 hours although the shelf life of the milk produced at room temperature was 24 hours, this is the average shelf life reported generally for most milk and milk-based products.</p><p>Keys: 5.0 = Very Good; 4.9 - 4.0 = Good; 3.9 - 3.0 = Fair; 2.9 - 2.0 = Poor; 1.9 - 1.0 = Bad.</p><p>This is probably the reason why several workers are exploring the use of chemical preservatives for prolonging the shelf life of milk products [<xref ref-type="bibr" rid="scirp.47721-ref20">20</xref>] [<xref ref-type="bibr" rid="scirp.47721-ref21">21</xref>] . Remarkable mouth feel, odour, colour, appearance and overall acceptability were recorded for all the milk samples. Soymilk and coconut milk were highly acceptable by the panelists at 0 hours of production of the yogurt but the mean scores for the aroma and visual appearance/colour of the yogurts decreased with storage time and so were the yogurts produced from cow and goat milk. One way Anova test of homogeneity shows no significant difference for general sensory evaluation of the milk samples at 5% confidence level as shown in <xref ref-type="fig" rid="fig1">Figure 1</xref>.</p></sec><sec id="s4"><title>4. Conclusion and Recommendation</title><p>The result revealed that milk prepared from coconut and soybean could be used as a beverage for both the young and old due to the high fat and protein contents. Based on the sensory evaluation, the soy milk and coconut milk were also acceptable. This indicates that utilization of soymilk and coconut milk may be enhanced when processed into beverage drinks. It is therefore suggested that milk from coconut and soya beans be encouraged so as to solve the problem of protein-calorie malnutrition in Africa in particular and the world in general. The nutritional values derived from proximate analysis of the milk samples are comparable. This clearly shows that either of the food can substitute for each other based on the values obtained from this study. Further study on microbial susceptibility and contaminants is required.</p><table-wrap id="table2"  position="float"><object-id pub-id-type="pii">Table 2</object-id><label>Table 2</label><caption><p>. pH of fermenting milk sample during yogurt production</p></caption><table><thead><tr><th align="center" valign="middle" >Milk type</th><th align="center" valign="middle" >pH of fresh milk yogurt at 0 hour</th><th align="center" valign="middle" >pH of fermenting milk after 48 hours</th><th align="center" valign="middle" >pH of fermenting milk after 72 hours</th></tr></thead><tbody><tr><td align="center" valign="middle" >Skimmed milk</td><td align="center" valign="middle" >5.37</td><td align="center" valign="middle" >5.10</td><td align="center" valign="middle" >4.09</td></tr><tr><td align="center" valign="middle" >Goat milk</td><td align="center" valign="middle" >5.24</td><td align="center" valign="middle" >4.99</td><td align="center" valign="middle" >4.28</td></tr><tr><td align="center" valign="middle" >Cow milk</td><td align="center" valign="middle" >5.85</td><td align="center" valign="middle" >5.31</td><td align="center" valign="middle" >5.11</td></tr><tr><td align="center" valign="middle" >Soy milk</td><td align="center" valign="middle" >5.73</td><td align="center" valign="middle" >5.33</td><td align="center" valign="middle" >5.07</td></tr><tr><td align="center" valign="middle" >Coconut milk</td><td align="center" valign="middle" >5.98</td><td align="center" valign="middle" >5.62</td><td align="center" valign="middle" >5.33</td></tr></tbody></table></table-wrap><table-wrap id="table3"  position="float"><object-id pub-id-type="pii">Table 3</object-id><label>Table 3</label><caption><p>. Mean scores for acceptability of all the milk samples</p></caption><table><thead><tr><th align="center" valign="middle" >Sample</th><th align="center" valign="middle" >Colour</th><th align="center" valign="middle" >Appearance</th><th align="center" valign="middle" >Odour</th><th align="center" valign="middle" >Mouth feel</th><th align="center" valign="middle" >Overall acceptability</th></tr></thead><tbody><tr><td align="center" valign="middle" >Skimmed Milk</td><td align="center" valign="middle" >4.1</td><td align="center" valign="middle" >4.3</td><td align="center" valign="middle" >5.0</td><td align="center" valign="middle" >4.8</td><td align="center" valign="middle" >4.6</td></tr><tr><td align="center" valign="middle" >Cow milk</td><td align="center" valign="middle" >4.0</td><td align="center" valign="middle" >4.2</td><td align="center" valign="middle" >4.8</td><td align="center" valign="middle" >4.6</td><td align="center" valign="middle" >4.3</td></tr><tr><td align="center" valign="middle" >Goat milk</td><td align="center" valign="middle" >4.2</td><td align="center" valign="middle" >4.4</td><td align="center" valign="middle" >4.7</td><td align="center" valign="middle" >4.1</td><td align="center" valign="middle" >4.3</td></tr><tr><td align="center" valign="middle" >Coconut milk</td><td align="center" valign="middle" >3.4</td><td align="center" valign="middle" >3.7</td><td align="center" valign="middle" >4.2</td><td align="center" valign="middle" >3.9</td><td align="center" valign="middle" >3.8</td></tr><tr><td align="center" valign="middle" >Soy milk</td><td align="center" valign="middle" >3.6</td><td align="center" valign="middle" >3.8</td><td align="center" valign="middle" >4.2</td><td align="center" valign="middle" >4.3</td><td align="center" valign="middle" >4.0</td></tr></tbody></table></table-wrap><fig id="fig1"><label>Figure 1</label><caption><p> Overall evaluation of yoghurt from milk varieties</p></caption><graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://file.scirp.org/Html/htmlimages\9-2701217x\26fc8354-8e49-4b9e-b428-7048d346bb31.png"/></fig></sec><sec id="s5"><title>Acknowledgements</title><p>The authors thank Mr. Abiola Adeosun for his assistance during this research.</p></sec></body><back><ref-list><title>References</title><ref id="scirp.47721-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">ETESHOLA, E. AND ORAEDU, A.C.I. (2010) FATTY ACIDS COMPOSITION OF TIGERNUT TUBERS (CYPERUS ESCULENTUS L.), BAOBAB SEEDS (ADANSONIA DIGITATA L.) AND THEIR MIXTURES. JOURNAL OF THE AMERICAN OIL CHEMISTS’ SOCIETY, 73, 255. HTTP://DX.DOI.ORG/10.1007/BF02523905</mixed-citation></ref><ref id="scirp.47721-ref2"><label>2</label><mixed-citation publication-type="other" xlink:type="simple">DOBRZANSKI, Z., KOLACZ, R., GORECKA, H., CHOJNACKA, K. AND BARTKOWIAK, A. (2005) THE CONTENT OF MICROELEMENTS AND TRACE ELEMENTS IN RAW MILK FROM COWS IN THE SILESIAN REGION. POLISH JOURNAL OF ENVIRONMENTAL STUDIES, L4, 685-689.</mixed-citation></ref><ref id="scirp.47721-ref3"><label>3</label><mixed-citation publication-type="other" xlink:type="simple">RAO, D.R. PULUSSANI, S.R. AND CHAWAN, C.B. (1998) PREPARATION OF A YOGURT-LIKE PRODUCT FROM COWPEA AND MUNG BEANS. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 23, 195-198.</mixed-citation></ref><ref id="scirp.47721-ref4"><label>4</label><mixed-citation publication-type="other" xlink:type="simple">REED, G. (1982) PRESCOT AND DUNN’S INDUSTRIAL MICROBIOLOGY. 4TH EDITION, MACMILLAN PUBLISHERS, LONDON, 146-173.</mixed-citation></ref><ref id="scirp.47721-ref5"><label>5</label><mixed-citation publication-type="other" xlink:type="simple">HUGHES, D.B. AND HOOVER, D.G. (1991) BIFIDOBACTERIA: THEIR POTENTIAL FOR USE IN AMERICAN DAIRY PRODUCTS. FOOD TECHNOLOGY, 45, 74-80</mixed-citation></ref><ref id="scirp.47721-ref6"><label>6</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>TERNA</surname><given-names> G. </given-names></name>,<name name-style="western"><surname> MUSA</surname><given-names> A. </given-names></name>,<etal>et al</etal>. (1998)<article-title>TERNA, G. AND MUSA, A.  SOYBEANS YOGURT PRODUCTION USING STARTER CULTURE FROM “NONO”</article-title><source>. NIGERIAN JOURNAL OF BIOTECHNOLOGY</source><volume> 9</volume>,<fpage> 17</fpage>-<lpage>23</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.47721-ref7"><label>7</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>AKOMA</surname><given-names> O.</given-names></name>,<name name-style="western"><surname> ELEKWA</surname><given-names> U.O.</given-names></name>,<name name-style="western"><surname> AFODUNRINBI</surname><given-names> A.T. </given-names></name>,<name name-style="western"><surname> ONYEUKWU</surname><given-names> G.C. </given-names></name>,<etal>et al</etal>. (2000)<article-title>AKOMA, O., ELEKWA, U.O., AFODUNRINBI, A.T. AND ONYEUKWU, G.C.  YOGURT FROM COCONUT AND TIGER NUTS</article-title><source>. THE JOURNAL OF FOOD TECHNOLOGY IN AFRICA</source><volume> 5</volume>,<fpage> 132</fpage>-<lpage>134</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.47721-ref8"><label>8</label><mixed-citation publication-type="other" xlink:type="simple">KESSLER, H.G. (1981) FOOD ENGINEERING AND DAIRY TECHNOLOGY. TRANSLATOR M. WOTZILKA, VERLAG A KESSLER, FREISING,</mixed-citation></ref><ref id="scirp.47721-ref9"><label>9</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>ALGANESH</surname><given-names> T.</given-names></name>,<name name-style="western"><surname> OFODILE</surname><given-names> L. </given-names></name>,<name name-style="western"><surname> FEKADU</surname><given-names> B. </given-names></name>,<etal>et al</etal>. (2007)<article-title>ALGANESH, T., OFODILE, L. AND FEKADU, B.  MICROBIAL QUALITY AND CHEMICAL COMPOSITION OF RAW WHOLE MILK FROM HORRO CATTLE IN EAST WOLLEGA, ETHOPIA</article-title><source>. AFRICAN JOURNAL ON LINE</source><volume> 3</volume>,<fpage> 1</fpage>-<lpage>10</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.47721-ref10"><label>10</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>AWORH</surname><given-names> S. </given-names></name>,<name name-style="western"><surname> AKINNIYI</surname><given-names> A. </given-names></name>,<etal>et al</etal>. (1989)<article-title>AWORH, S. AND AKINNIYI, A.  PROXIMATE ANALYSIS AND NUTRITIONAL COMPONENT OF LOCALLY PRODUCED CHEESE</article-title><source>. NIGERIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY</source><volume> 9</volume>,<fpage> 220</fpage>-<lpage>227</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.47721-ref11"><label>11</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>HEANEY</surname><given-names> R.P. </given-names></name>,<name name-style="western"><surname> WEAVER</surname><given-names> C.M. </given-names></name>,<etal>et al</etal>. (1990)<article-title>HEANEY, R.P. AND WEAVER, C.M.  CALCIUM ABSORPTION FROM KALE</article-title><source>. THE AMERICAN JOURNAL OF CLINICAL NUTRITION</source><volume> 51</volume>,<fpage> 656</fpage>-<lpage>657</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.47721-ref12"><label>12</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>AJAI</surname><given-names> A.I.</given-names></name>,<name name-style="western"><surname> OCHIGBO</surname><given-names> S.S.</given-names></name>,<name name-style="western"><surname> NDAMITSO</surname><given-names> M.M. </given-names></name>,<name name-style="western"><surname> OLA</surname><given-names> O.J. </given-names></name>,<etal>et al</etal>. (2012)<article-title>AJAI, A.I., OCHIGBO, S.S., NDAMITSO, M.M. AND OLA, O.J.  PROXIMATE AND MINERAL COMPOSITION OF DIFFERENT RAW COW’S MILK IN MINNA</article-title><source>. EUROPEAN JOURNAL OF APPLIED ENGINEERING AND SCIENTIFIC RESEARCH</source><volume> 1</volume>,<fpage> 23</fpage>-<lpage>29</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.47721-ref13"><label>13</label><mixed-citation publication-type="other" xlink:type="simple">DAVID, F. (1986) HUMAN NUTRITIONAL DIETARY. FOOD ENCYCLOPEDIA, 35-36.</mixed-citation></ref><ref id="scirp.47721-ref14"><label>14</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>BELEWU</surname><given-names> M.A.</given-names></name>,<name name-style="western"><surname> BELEWU</surname><given-names> K.Y. </given-names></name>,<name name-style="western"><surname> OLATUNJI</surname><given-names> S.A. </given-names></name>,<etal>et al</etal>. (2005)<article-title>BELEWU, M.A., BELEWU, K.Y. AND OLATUNJI, S.A.  PREPARATION, COMPOSITIONAL AND ORGANOLEPTIC QUALITIES</article-title><source>. BIOSCIENCE RESEARCH BULLETIN</source><volume> 21</volume>,<fpage> 129</fpage>-<lpage>137</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.47721-ref15"><label>15</label><mixed-citation publication-type="other" xlink:type="simple">FRAIZER, W.C. AND WESTHOFF, D.C. (1985) FOOD MICROBIOLOGY. MCGRAW HILL INC., BOSTON, 278.</mixed-citation></ref><ref id="scirp.47721-ref16"><label>16</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>BELEWU</surname><given-names> M.A.</given-names></name>,<name name-style="western"><surname> BELEWU</surname><given-names> K.Y. </given-names></name>,<name name-style="western"><surname> BAMIDELE</surname><given-names> R.A. </given-names></name>,<etal>et al</etal>. (2010)<article-title>BELEWU, M.A., BELEWU, K.Y. AND BAMIDELE, R.A.  CYPER-COCONUT YOGHURT: PREPARATION, COMPOSITIONAL AND ORGANOLEPTIC QUALITIES</article-title><source>. AFRICAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY</source><volume> 1</volume>,<fpage> 10</fpage>-<lpage>12</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.47721-ref17"><label>17</label><mixed-citation publication-type="other" xlink:type="simple">SIMOPOULOS, A. AND DE MEESTER, F. (2009) A BALANCED OMEGA 6/OMEGA3 FATTY ACID RATIO, CHOLESTEROL AND CORONARY HEART DISEASES. WORLD REVIEW OF NUTRITION AND DIETETICS, 91, 100.</mixed-citation></ref><ref id="scirp.47721-ref18"><label>18</label><mixed-citation publication-type="other" xlink:type="simple">REED, G. (1982) PRESCOT AND DUNN’S INDUSTRIAL MICROBIOLOGY. MACMILLAN PUBLISHERS, LONDON, 146-173.</mixed-citation></ref><ref id="scirp.47721-ref19"><label>19</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>UABOI-EGBENNI</surname><given-names> P.O.</given-names></name>,<name name-style="western"><surname> OKOLIE</surname><given-names> P.N.</given-names></name>,<name name-style="western"><surname> AKINTUNDE</surname><given-names> T.I.</given-names></name>,<name name-style="western"><surname> BISI-JOHNSON</surname><given-names> O.</given-names></name>,<name name-style="western"><surname> ENWE</surname><given-names> L. </given-names></name>,<name name-style="western"><surname> BESSONG</surname><given-names> P.O. </given-names></name>,<etal>et al</etal>. (<year>2010</year>)<article-title>PROXIMATE ANALYSIS AND MICROBIOLOGICAL QUALITY OF CHEESE PRODUCED FROM RAW COW MILK OBTAINED FROM FULANI SETTLEMENT IN OGUN STATE, NIGERIA USING LACTIC ACID BACTERIA AND EXTRACT FROM SODOM APPLE LEAF</article-title><source>. PAKISTAN JOURNAL OF NUTRITION</source><volume> 9</volume>,<fpage> 920</fpage>-<lpage>925</lpage>.<pub-id pub-id-type="doi">HTTP://DX.DOI.ORG/10.3923/PJN.2010.920.925</pub-id></mixed-citation></ref><ref id="scirp.47721-ref20"><label>20</label><mixed-citation publication-type="other" xlink:type="simple">SUMATI, R.M. AND SHALINI, M.R. (1986) FOOD SCIENCE WILEY EASTERN LIMITED, BOMBAY, 196-207.</mixed-citation></ref><ref id="scirp.47721-ref21"><label>21</label><mixed-citation publication-type="other" xlink:type="simple">URIAH, N. AND IWAGBE, Y. (1990) PUBLIC HEALTH. FOOD MICROBIAL, 19-54.</mixed-citation></ref></ref-list></back></article>