Doum powder was obtained from the local market in Alexandria, Egypt Alloxan was obtained from Alpha Co. (Mumbai, India) and was dissolved in saline solution (0.9% NaCl, pH 7). The dose of alloxan (120 mg/kgof BW per day) was chosen to induce diabetes in rats in Egypt according to [13] [14]. All chemicals and reagents used in this study were of analytical grade and sigma co.

The fruit was cleaned, debris removed and then separated into pulp and seed. The pulp was dried and ground into fine powder. The doum fruit extract was made by boiling 10 g of the powdered pulp in 100 ml of distilled water for 2 min [15]. Then filtered with Whatman filter paper no.1 and stored at 4˚C.

Thirty male albino rats (150 - 160 g weight) obtained from the department of home economics, faculty of agriculture, Alexandria University, Egypt. The design of the experiment was approved by the local committee in Alexandria University, Egypt, according to the protocol conforms to the guidelines of the National Institutes of Health (NIH). The animals were housed in cages under proper environmental conditions. Animals were housed 5 per cage and fed a commercial diet. Rats were fed pellets consisting of 30% berseem (Trifolium alexandrinum) hay, 25% yellow corn, 26.2% wheat bran, 14% soybean meal, 3% molasses, 1% CaCl₂, 0.4% NaCl, a mixture of minerals (dicalcium phosphate, 45.8 mg; magnesium sulfate, 15 mg; manganese sulfate, 6.15 mg; ferric sulfate, 50 mg; potassium iodide, 0.392 mg; copper sulfate, 5 mg; cobalt sulfate, 0.1 mg; and zinc sulfate, 5 mg/kg pellets) and vitamins (6000 IU of vitamin A, 40 mg of vitamin E, 6 mg of vitamin C, 4 mg of vitamin B2, 3 mg of folic acid, 0.05 mg of niacin, 900 IU of vitamin D3, 2 mg of vitamin K3, 2 mg of vitamin B1, 2 mg of vitamin B6, 0.05 mg of biotin, 10 mg of calcium pantothenate/kg of pellets), and 0.1% methionine. The chemical analysis of the pellets showed that they contained 17.5% CP (method 988.05) [16], 14.0% crude fiber (method 989.03) [16], 2.7% crude fat, and 2200 kcal/kg of diet (method 920.39) [16]. Tap water was provided ad libitum and rats were acclimated for 2 wk at 25˚C ± 1˚C with a 12-h dark and light cycle. After two weeks of acclimatization.

The animals were divided into five equal groups 6 animals in each group. Group 1 was used as a control. Group 2 diabetic rats; group 3, 4 and 5 were made diabetic and were treated with Hyphaene thebaica, daily at a dose of (0.5 g, 1 g, and 2 g/kg b∙wt.) respectively, by intragastric tube. The tasted doses for Hyphaene thebaica were given every other day by stomach tube for 6 week by The rats were observed for signs of toxicity and mortality for the first 4 hr and for 24 hr.

Blood samples were collected in dry test tubes and centrifuged at 860 ×g for at 4˚C for 20 minutes. The obtained plasma was kept at −80˚C for analysis [17].

Plasma samples were analyzed for glucose, total lipids, cholesterol, triglycerides (TG) and lactate dyhadreygenase Activity (LDH) according to the methods of [18] [19] [20] [21] [22], respectively. High-density lipoprotein-cholesterol (HDL-c) was determined according to the methods of [23] [24]. Low-density lipoprotein-cholesterol (LDL-c) was determined by calculation (cholesterol-(TG/5+HDL).

The method of [25] was used to assay the activities of ALT and AST, whereas the ALP activity was determined according to the methods of [26].

Antioxidant Parameters like Catalase (CAT) enzyme and Malondialdehyde Activity were determined according to the methods of [27] [28].

Specimens from pancreas were collected from all experimental groups and fixed in 10% neutral buffer formalin, dehydrated in ethyl alcohol and cleared in xylene. 4 - 5 μ thick sections were prepared and stained with haematoxylin and Eosin [29].

The data were statistically analyzed according to [30]. Statistical significance of the difference in values of control and treated animals was calculated by F test with 1% and 5% significance level. Then, the data were statistically tested by using Duncan’s Multiple Range Test [31].

The data in Table 1 showed a significant increase in blood glucose level in diabetic rats of group 2 compared to that of normal control rats (group 1). While administration of doum extract caused significant decreases in blood glucose levels in rats of groups 3, 4 and 5 as compared to the diabetic rats. The increase in blood glucose may indicate disrupted carbohydrate metabolism due to enhanced breakdown of liver glycogen, possibly mediated by increase in adrenocorticotrophic and glucagon hormones and/or reduced insulin activity. These results are in agreement with those found by [32] who mentioned that giving doum extract orally led to significant decrease in blood glucose.

The data in Table 1 also, show that the levels of Insulin level in rats of groups 4 and 5 treated of doum extract decreased to be near that of the control group. Therefore, it seems that doum extract has an effective ability to keep the Insulin hormone at normal levels [11] reported that glucose levels were increased with a decrease in insulin levels, which was to due to the fact that type 2 diabetes mellitus was linked to reduced insulin release or insulin resistance, impaired glucose, lipid metabolisms, Also as activation of oxidative stress indices.

The hyperglycemia leads to an imbalance in the metabolism of carbohydrates and to inhibition of glucose utilization in animal body tissues such as muscles and fatty, etc. [33]. Therefore diabetes causes a significant increase in blood glucose and may thus raised hepatic glycogenolysis and gluconeogenesis as well as it reduces the transport of glucose from the blood to the tissues of the body.

Values are expressed as means ± SE. Mean values within a row not sharing a common superscript letters (a, b) were significantly different, p < 0.05.

The doum extract has an effective role in reducing blood glucose as it plays the role of insulin when glucose enters the cells and achieving optimal use of glucose [12].

Effect of doum fruit extract on blood lipid profile is presented in Table 2. The results indicated that Levels of total lipid, triglyceride, Total cholesterol and LDL were higher in diabetic rats compared with normal control rats. While treatment with doum extract significantly (P < 0.05) decreased in the total lipids, triglyceride, total cholesterol and LDL of all treated groups as compared to the diabetic rats. On the other hand the value of HDL increased in all treated groups as compared with normal control and diabetic rats. Diabetes causes lipid metabolism due to insulin deficiency in the body resulting in damage to pancreatic cells. Insulin can activate are act as glucosidase inhibitors, reducing the lipoprotein lipase, the enzyme lipoprotein solver. These results are in agreement with other studies [32] [34].

Also observed that rats treated with doum extract showed a decrease in the level of fats and triglycerides and an increase in HDL-cholesterol at higher concentrations 250 mg/kg body weight, It is known that high levels of blood cholesterol and LDL are risk factors for cardiovascular disease, while HDL is involved in reverse cholesterol transport carring, cholesterol and cholesterol esters from the peripheral tissues and cells to the liver, where cholesterol is metabolized into bile acids, which reduces tissues cholesterol levels [35]. These results confirmed that the doum fruit is good source of flavonoids which are considered very important effective protect against cardiovascular diseases [36] in addition to enhancing the metabolic processes of carbohydrates due to their high content of bioactive compounds, such as polyphenols, flavonoids and carotenoids [37].

Data presented in Table 3 showed the effect of the doum fruit (Hyphaene thebaica) extract on liver enzymes. The highest AlT, AST, ALP and LDH activity

Values are expressed as means ± SE. Mean values within a row not sharing a common superscript letters (a, b) were significantly different, p < 0.05.

Values are expressed as means ± SE. Mean values within a row not sharing a common superscript letters (a, b) were significantly different, p < 0.05.

was found in blood plasma of the diabetic rats group. Treatment with Hyphaene thebaica extract 1 and 2 g/kg b∙wt significantly (P < 0.05) decreased the levels of ALP and LDH Meanwhile no significant (P > 0.05) difference was found in ALT and AST among all treated groups as compared to the diabetic rats. These results are in agreement with those of [38] [32].

The liver is the largest most complex organ in the body and plays major roles in a wide variety of biochemical functions of the body. Therefore, diseases of the liver will markedly affect the health [39].

Liver enzymes (ALT, AST and ALP) activities are important vital indicators for detecting liver toxicity, the increased levels of serum enzyme such as AST and ALT indicates that necrosis or damage to the liver cells [40].

The previous studies indicated that the doum fruit extract had no any harmful effect on the liver, but it improves the functions of enzymes (ALT, AST and ALP) activities. Research has focused on the nutritional value of it as it contains trace minerals and fatty acids, the most important of which is an essential important linoleic acid for the body and the doum extract is also rich in antioxidants as well as a large amount of water-soluble phenolic contents, Due to the high nutritional value, it is therefore used in treating different health problems and improving liver and kidney functions [15] [35].

LDH is the cytoplasm enzyme and is found in all cells of organs like organs, including the heart, the liver, the kidneys, the brain, red blood cells and the lung. It is used as an indication of inflammation or tissue damage. Also an increase in the serum causing cardiomyopathy, Studies have indicated that it increases significantly in diabetic rats [41] [42] [43].

Data recorded in Table 4 shows results of CAT activity in blood plasma and effect of the doum fruit extract on CAT activity. The lowest CAT activity was found in blood plasma of the diabetic group. Treatment with doum fruit extract led to significantly (P < 0.05) increased CAT activity compared with the diabetic rats group. While MDA activity groups were significantly (P < 0.05) decreased in plasma of rats treatment with doum fruit extract. This was attributed to the rich amount of flavonoids found in Hyphaene thebaica, which inhibits lipid oxidation by scavenging free radicals or by other mechanisms, such as singlet oxygen quenching, metal chelation, and lipoxygenase inhibition [15]. As well as the aqueous extract of Hyphaene thebaica which possesses an antioxidant activity due to its substantial amount of water-soluble phenolic content. These results are in agreement with other studies [34] [38].

As well doum contains high levels of phenol and flavonoids that possess antioxidant properties as it is known that phenolic compounds act as antioxidants due to their ability to give the hydrogen molecule and in addition to molecule inhibiting lipid oxidation by scavenging radicals, singlet oxygen quenching, metal chelation, and lipooxygenase inhibition [44].

The histological struture of the control rat pancreas shown that (Figure 1) shows normal histological structure of the island of langerhans cells, Also, It was found that lobules are composed largely of grape-like clusters of exocrine cells called acini, which secrete digestive enzymes embedded within the pancreatic exocrine tissue are islets of langerhans, the endocrine component of the pancreas.

(Figure 2) It shows that injection of alloxan effect in rats (group 2) caused shrinkage and vacuolizations of some cells in islets of langerhans of both alpha

Values are expressed as means ± SE. Mean values within a row not sharing a common superscript letters (a, b) were significantly different, p < 0.05.

and beta as well as found necrotic in beta Langerhans This may be due to the fact that diabetes affects the lipid metabolism due to insulin deficiency in the body, which leads to necrosis and damage to beta langerhans cells [34].

Treatment of alloxan diabetic rat (group 3) with doum fruit extract (0.5 g/kg b∙w) showed the size of islets of langerhans cells was moderate and slight congestion between acinar cells as observed (Figure 3). However treatment with doum fruit extract (1 and 2 g/kg b∙w) caused marked improvement in the islets form of Langerhans cells and becoming to normal acinar cells and normal histological structure of pancreatic tissue as shown (Figure 4 and Figure 5). The hypoglycemic effect on blood glucose level could be through increased serum insulin levels provided by repair/regeneration of the endocrine pancreas [45].