Thirty each of hard cheese (Ras), and processed cheese was randomly collected as sold to consumers from dairy shops in Cairo and Giza governorates.

Titratable acidity percentage was measured according to [2] and NaCl content according to Volhard method [3] .

Preparation of decimal dilutions for the collected samples, Total coliform count with Completed test for E. coli, Total proteolytic count, Lipolytic microorganisms count, Enterococci count, Total Yeast and Total Mold count were examined according to [3] with bio chemical identification of the isolated Coliforms & Enterococci species were examined according to [4] . While yeast species were examined according to [5] .

Titratable acidity percentage: Increase of titratable acidity is a monitor of freshness and the bacterial activity in milk [1] [6] . While lower pH of milk to less than 4.6 protects the fermented dairy products from contamination with pathogens and considers safe hygienically [7] [8] . Data recorded in Table 1 showed that the mean titratable acidity percentage of the examined Ras and Processed cheese samples were 0.5 ± 0.073 and 0.93% ± 0.062%, respectively. Nearly similar

findings of Ras cheese were obtained by [9] . Higher results of processed cheese were reported by [10] . Whereas, lower findings of Processed cheese were obtained by [9] [11] .

Salt percentage: Salt added is an important step in most cheese varieties; it enhances flavor, draws the whey out of the curd and controls the undesirable microorganisms. The salt content of cheese ranges from about 0.5% to 0.7% (w/w) in acid curd varieties, such as cottage cheese to about 4% to 6% (w/w) in pickled cheeses [12] [13] . Regarding the salt percentage in examined cheese samples (Table 1), it ranged from 2.8 - 4.7 and 0.51 - 1.4 with a mean value of 3.4 ± 0.078 and 1.03% ± 0.05% in the examined samples of Ras cheese and Processed cheese, respectively. Nearly similar results in processed cheese were recorded by [9] . While lower obtaining of Ras cheese were recorded by [9] .

The variation of salt content in the examined cheese samples is due to the added salt depends on individual dairies, and there is no standard in addition of salt to cheese and the amount of salt relies on discretion of cheese maker [12] . The high salt content in some examined samples can mask the poor bacteriological quality of the raw milk and prevent gas holes formation and off-flavor especially in summer [14] .

Coliforms contents (MPN/g): Coliform count is an indicator of possible fecal contamination, microbial quality and reflects the hygienic conditions in the food processing [15] . Regarding the results represented in Table 2, coliform could be detected in all of the examined samples of Ras cheese and Processed cheese, with mean values of 1.2 × 10¹⁰ ± 3.3 × 10⁹ and 2.5 × 10⁹ ± 6.5 × 10⁸ MPN/g in Ras and Processed cheese, respectively.

Lower results of processed cheese were reported by [9] [16] [17] . Lower results

of Ras cheese were reported by [9] [17] . The results revealed that all of the examined Ras cheese and processed cheese contain high numbers of coliform. Egyptian Standards recommend the count should be less than 10 cells/g [15] , the obtained results makes these cheeses of bad quality and cause losses economically [18] .

Isolated coliforms: Biochemical identification of Coliform organisms in Ras cheese, results revealed that Enterobacter intermedius (37.1%) was the most frequent one followed by Citrobacter diversus (16.13%), Citrobacter freundii (12.9%) and Klebsiella oxytoca (11.29%), while E. coli could be detected only in one isolate. Enterobacter intermedius (21.43), E. coli (20%) & Citrobacter freundii (20%) had the highest incidence, then Klebsiella oxytoca (12.86%), Citrobacter diversus (11.4%) and Erwinia chrysanthemi (7.14%) in the examined Processed cheese (Table 3).

E. coli was found in Ras cheese and processed cheese with percentages of 1.61 and 20%, respectively. E. coli is due to fecal contamination, indicates that other pathogens of fecal origin may be found [19] . Results presented in Table 4 illustrated the serological identification of the suspected isolates of E. coli by slide agglutination test. In Ras cheese, the suspected isolates were negative, while in processed cheese one strain out of 14 strains was positive and classified as E. coli O₂₅. Escherichia coli is present in the gastrointestinal tract of man and dairy animals. Milk can be contaminated either directly through animal feces or indirectly through contaminated surrounding environment, workers, and equipments. Most strains of E. coli are harmless commensals; some causes human gastrointestinal disease with mild to severe symptoms that may be fatal in high-risk individuals. Moreover, E. coli was reported for cases of pyelonephritis cystitis, pyelitis, peritonitis and appendicitis [20] [21] .

Total yeast count: Yeast contamination is economically hazard as it causes

objectionable changes as yeasty flavor and frothy consistency. Some species of yeast cause public hazard as endocarditis, gastrointestinal disturbance, and sometimes, fatal systemic diseases [22] . Data in Table 2 revealed that contaminated yeast could be detected in 30 (100%) and 25 (83.3%) of Ras cheese and Processed cheese, respectively, with mean values of 4.9 × 10⁷ ± 5.6 × 10⁶ and 2.57 × 10⁵ ± 1.8 × 10⁵ cfu/g, respectively. Nearly similar results of Ras cheese were recorded by [9] [17] , while lower findings were recorded by [23] . Lower results of Processed cheese were obtained by [11] [24] , while higher results were recorded by [9] [17] . According to the Egyptian standards the count of total viable yeast should not exceed 100 cfu/g in hard cheese [25] , 100% of Ras cheese samples was disagreed with this standard. This high level may be due to poor hygienic measures during production or using raw materials of bad quality [26] .

Isolated yeast species: from Figure 1, the obtained results of cheese proved that C. albicans; C. diddensiae; C. intermedia; C. mesenterica; C. parapsilosis; C. zeylanoides; Cryptococcus curvatus; Debaryomyces hansenii; Db. Accidentalis; Filobasidiumcapsuligenum; Gal. Geotrichum; Geo. fermentans; Kluyveromyces marxianus; Lachanceacidri; Leucosporidiumscottii; P. guilliermondii; Schizosaccharomyces pombe; S’ copsisvini and Zygoascushellenicus were isolated from the examined samples of Ras cheese with concern that Gal. geotrichum (16.98%) and Schizosaccharomyces pombe (15.09%) had the highest incidence among the isolated strains. On Processed cheese, C. albicans (13.34%) was the most frequent one then Schizosaccharomyces pombe (11.13%); C. catenulate (6.67%); Cryptococcus curvatus (6.67%); Kluyveromyces marxianus (6.67%) and Lipomyces lipofer (6.67%). High acidity, low moisture content, storage at low temperature, and high salt concentration enhance growth of yeast. These spoilage yeast cause gas production, discoloration, soften texture, and fruity, bitter or yeasty off flavors [27] [28] . Candida spp. are the most common fungal infection in immune compromised persons known as candidiasis, which caused by C. albicans [29] [30] .

Total mold count: Presence of mold cause economic losses because it is accompanied by visible spoilage discoloration, off flavor, and rejection of the product, and may be a source of mycotoxins result in outbreaks of human food poisoning and many diseases as cancer, leukemia, and kidney toxicity [31] [32] . Regarding the data recorded in Table 2 mold was present in 33.3% and 56.67% of the examined Ras cheese and Processed cheese samples, respectively, The counts were ranged from 5 × 10³ - 4 × 10⁶ and 200 - 10⁷ cfu/g, with mean values of 3.6 × 10⁵ ± 2.5 × 10⁵ and 6.7 × 10⁵ ± 6.2 × 10⁵ cfu/g, respectively. Nearly similar

findings of Processed cheese were reported by [9] [17] , while Lower results of Processed cheese were recorded by [23] [24] . Nearly similar findings of Ras cheese were recorded by [17] . Higher obtaining of Ras cheese was recorded by [9] , while lower outcomes were reported by [23] . It was found that 33.3 and 56.67% of the examined Ras cheese and Processed cheese, respectively were highly contaminated with mold and disagree with the Egyptian standards [15] [33] because of the warm weather and inadequate refrigeration, increased species diversity [34] . The fungi contributed to poor cleaning and unhygienic measures during production and handling and post processing contamination [35] [36] .

Total proteolytic count: Bacillus spp. can survive heat treatment of milk [37] [38] . They can produce thermo stable hydrolytic enzymes are produced by Bacillus spp [39] [40] . B. stearothermophilus, B. licheniformis, B. coagulans, B. cereus, B. subtilis and B. circulansare the most commonly isolated species. Moreover, Bacillus species such as B. cereus, B. licheniformis and B. subtilis can produce many types of toxins associated with food borne diseases [41] [42] . The proteolytic enzymes may cause undesirable changes in texture [25] [43] and in flavor (especially bitterness). According to the obtained results in (Table 2) it was cleared that proteolytic organisms were present in 30 (100%) and 18 (60%) of the examined Ras cheese and Processed cheese, with a mean count of 5.8 × 10¹⁰ ± 4.5 × 10¹⁰ and 6.3 × 10⁶ ± 2.8 × 10⁶ cfu/g, respectively. Lower results of Ras & Processed cheese were obtained by [11] [44] .

Total lipolytic count: Psychrophilic bacteria like Pseudomonas, Enterobacter, Alcaligenes and some spore-formers produce extracellular lipase enzyme

that is adsorbed on milk fat globules and concentrated in the manufactured cheese. These enzymes hydrolyze triglyceride fat constituents of short chain fatty acids, including butyric, caproic, caprylic, and capric acids resulting in unpleasant odors in milk and rancidity in cheese [39] [45] . Results detected in Table 2 revealed that lipolytic organisms were present in Ras and Processed cheese samples in percentages of 100% and 40%, respectively, with a mean count of 3 × 10⁸ ± 1.2 × 10⁸ and 16.8 × 10³ ± 5.4 × 10³ cfu/g, respectively. Lower results of Ras & Processed cheese were obtained by [11] [44] .

Total Enterococci count: Enterococci are useful indicator to the hygienic conditions during food manufacture. The presence of specific virulence factors in some strains of Enterococcus faecalis and to a lesser extent in strains of Enterococcus faecium makes them pathogenic to human [46] [47] . Using contaminated water supply, equipment and unhygienic conditions during the production, handling, and milk processing are the causes for their presence [48] [49] [50] . The given results recorded in Table 2 illustrated that Enterococci were isolated from all examined cheese samples with a mean value of 7.7 × 10⁸ ± 7.2 × 10⁸ and 4.3 × 10⁸ ± 2.7 × 10⁸ cfu/g in the examined Ras and Processed cheese samples, respectively. Lower results of Ras cheese were recorded by [17] . Nearly similar findings of processed cheese were recorded by [17] , whereas lower results were recorded by [51] .

Isolated Enterococci: The biochemical identification of Enterococci isolates in the examined cheese samples showed that Ent. durans (22.42%), Ent. ratti (20.69%) and Ent. faecium (17.24%) had the highest incidence, then Ent. faecalis (13.79%), Ent. italicus (13.79%) and Ent. caccae (8.63%) in the examined Ras cheese samples. Ent. durans was the most frequent one in processed cheese samples, followed by Ent. caccae (16.22%), Ent. faecium (10.81%), Ent. ratti (10.81%) and Ent. italicus (8.1%) (Figure 2).

Some enterococci of dairy origin have been recorded to the production of bacteriocins (enterocins) which has inhibitory effect to some pathogenic bacteria (Listeria monocytogenes and Salmonella spp.) [52] . Some studies have reported that E. faecium and E. faecalis may be a potential recipient of vancomycin resistance genes, so, [49] has recommended that E. faecium should not be considered as probiotics for human use. Also, Enterococci are pathogens causing endocarditis, bacteraemia, and urinary tract infections [16] .