Silver nitrate (AgNO₃) and acetone were obtained from Sigma Aldrich (Brazil). Clove (Syzygium aromaticum L.) essential oils were purchased from the local market of Florianópolis (Santa Catarina, Brazil). All chemicals used were of analytical grade, and the solutions were prepared with deionized water.

Initially, the clove essential oil was diluted in acetone at a ratio of 1:170. A stock solution of 0.31 mmol.L⁻¹ silver nitrate (AgNO₃) was prepared. Four aliquots of this solution were collected, and their pH was adjusted to 7, 8, 9 and 10 with a 0.1 mol.L⁻¹ NaOH solution in order to evaluate the synthesis under the different conditions. A 30-mL aliquot of each solution was heated under constant magnetic stirring, and 2 mL of the diluted clove essential oil was added dropwise into the boiling solution. Afterward, the solution remained under heating and vigorous stirring for 30 min. A brown-yellow color was observed indicating the formation of silver nanoparticles (AgNP).

The UV-Vis spectroscopy absorption study was performed on a Hitachi spectrophotometer, model U-1800 (Tokyo, Japan) at the wavelength range of 300 to 800 nm. The readings were performed at ~25˚C, and the results were used to build the curves of the Surface Plasmon Resonance (SPR) bands of the samples.

For the dynamic light scattering analysis, the AgNPs samples were evaluated in a Zetasizer Nano Series (Malvern Instruments Worcestershire, UK) at a fixed angle of 173˚ at room temperature (25˚C). The results of particle size (Z-ave), polydispersity index (PI) and the zeta potential (z) were obtained.

Transmission electron microscopy was performed to elucidate the morphology of AgNPs in the different synthesis conditions. A JEOL model JEM-1011 (Tokyo, Japan) microscope was used for the TEM evaluation under 80 kV. The obtained images and the size distribution of the particles were analyzed with the aid of the Quantikov Image Analyzer software.

The evaluation of the antimicrobial activity of AgNPs was performed according to a methodology adapted from the Clinical and Laboratory Standards Institute (CLSI) [12] . The AgNP samples were diluted with ultrapure water to obtain solutions at final concentrations in the wells of 40, 60, 80 and 100 μL∙mL⁻¹. The following bacterial cultures were used: Escherichia coli (ATCC 8739) (gram negative), Staphylococcus aureus (ATCC 25923) (gram positive) and Bacillus cereus (ATCC 10987) (gram positive). Bacterial cells were suspended in 0.9% (w/v) saline solution (NaCl) and the inoculum density was standardized using the McFarland turbidity comparison standard to achieve a final plate concentration of ~10⁵ CFU/mL.

Sterile 96-well polypropylene microplates were used for analysis. In each well were added 5 μl of inoculum, 90 μL of Mueller-Hinton broth and 10 μL of nanoparticles at different concentrations. Plates were incubated at 35˚C for 16 - 20 h. Afterward, the turbidity was observed (to the naked eye) and compared to the negative control (without inoculum) and to the positive control (without nanoparticles), being considered efficient in controlling the microorganism when the turbidity was not observed.

All assays were performed at least in triplicate, and the data were expressed as mean and standard deviation.

The samples were characterized for the elucidation of their size, polydispersity index and zeta potential by DLS, while the morphology of silver nanoparticles was studied by transmission electron microscopy (TEM) to verify their shape. The results of the DLS measurements are shown in Table 1.

The mean size obtained by DLS indicates the hydrodynamic radius of the particle. A higher deviation is verified for the sample AgNP_pH 7, confirming what was observed in the SPR band. The AgNP_pH 10 sample showed the smallest average particle size; however, the highest polydispersity index (PI) was also observed for this sample. In this case, PI values lower than 0.4 indicate populations with a narrower distribution [15] . This result reinforces the results observed in the UV-Vis analysis. All the AgNP samples showed zeta potential values between −30.1 and −50.8 mV indicating good stability against agglomeration.

Figure 2 shows the micrographs of AgNP obtained by TEM. Dense particles of various shapes and a thin layer on the surface of silver nanoparticles were observed. This may be evidence of a binding between the oil’s phytochemicals, responsible for promoting steric hindrance stability. The different particle shapes observed herein can be attributed to the pH of the solutions that influences the

reduction and stabilization of the nanoparticles system [16] [17] .

The sample AgNP_pH 7 (Figure 2(a)) showed mainly triangles, squares, hexagons, and spheres, while pentagons, hexagons, and spherical shapes were observed for AgNP_pH 8 (Figure 2(b)), and little number of triangular shapes. In AgNP_pH 9 (Figure 2(c)) the spherical shapes were predominant, but a larger agglomeration of the particles was observed, which in this case, can contribute to the coalescence of the particles. The largest variety of shapes was observed in AgNP_pH 10 (Figure 2(d)) which presented hexagon, square, spherical and needle formats.

The micrographs were analyzed to obtain the size distribution with the aid of the Quantikov Image Analyzer software, and the histograms of size distribution are represented in Figure 3. Overall, the samples presented a mean particle diameter between 27 to 94 nm. The AgNP_pH 7 sample had the highest average diameter and a substantial heterogeneity of sizes (78.0 ± 16.3 nm), this result reinforces what was observed by UV-Vis (Figure 1) and resembles the results obtained by DLS (Table 1). The lowest mean diameter was observed for the sample AgNP_pH 8 (35.5 ± 8.5 nm), which showed 76% of the particles with a diameter in the 40 nm range, showing a high homogeneity in its particle sizes. The AgNP_pH 9 sample had a mean diameter of 44.0 ± 18.9 nm indicating a high degree of heterogeneity like AgNP_pH 7, which can be evidenced in TEM micrographs (Figure 2). Furthermore, the average diameter of the AgNP_pH 10 sample (37.9 ± 18.7 nm) was quite similar to that obtained by DLS (Table 1), and its high standard deviation indicates the significant dispersion of the particles, with a great variety of shapes, which reinforces the results of SPR and TEM.

The broth microdilution test was used to determine the minimum inhibitory concentration (MIC) of the AgNPs. Turbidity was the parameter used to verify the growth of the microorganism in the wells. The sample AgNP pH 7 presented MIC of 60 μL/mL against E. coli, which is the same found for AgNP pH 8. For S. aureus the MIC of AgNP pH 8 was 80 μL/mL. The AgNP pH 9 sample presented a MIC of 100 μL/mL for both microorganisms. At pH 10 no inhibition of bacterial growth was observed. This result can be attributed to the higher variation on the particles’ shapes and lower volume of formation in this condition. Additionally, inhibition did not occur in any of the samples tested against B. cereus. Reference [18] reports that triangular shaped particles exhibit almost total inhibition of bacterial growth, which decreases for spherical-shaped particles and is even smaller for the stem-shaped particles. It is also reported antimicrobial activity of silver nanoparticles against S. aureus and E. coli by the agar diffusion method [4] . Similar results are demonstrated by [13] against the same bacteria using polymer films impregnated with silver nanoparticles. The MIC of 8 μg/mL is reported for nanoparticles measuring ~20 nm against E. coli [7] . The results observed in this study are consistent with those found in the literature, regarding the particularities of particle size and shape.