Test compounds (see Table 1), were dissolved in nutrient media with the aid of either ultrasound or gentle heating. In vitro serial dilutions of each compound

were prepared in the concentration range of 12.5 to 100 µg∙mL⁻¹. C. vulgaris was grown in nutrient broth medium (Gibco). Samples were incubated at 25˚C for 10 days in test tubes containing 4.0 × 10⁴ colony forming units (CFU) with continuous cold white fluorescent light, each assay was performed two times.

The growth of C. vulgaris was assessed by turbidity measured spectrophotometrically at 600 nm.

Percentage inhibition was obtained according to:

I % = T S − T C / ( 100 − T C )

where T_S is the sample transmittance and T_C is the control transmittance. Each value corresponds to the mean of three samples; replicate values showed errors below 5% in all cases. The data were analyzed by one. way ANOVA.

30 seeds each of barley (H. vulgare L.), wheat (Triticum aestivum), oat (Avena sativa), rye (Secale cereale L. cv), maize (Zea Mays L.) and Lollium rigidum were uniformly placed in Petri dishes covered with cotton (five Petri dishes for each assay with each species). In order to maintain individual test compound concentration each plate was watered with 10 mL of aqueous solution in the concentration range of 100 to 250 µg∙mL⁻¹ (see Table 2). The plates were sealed and incubated at 26˚C ± 2˚C in a 10 h: 14 h light-dark cycle for six days. Controls were

Nd = not determined.

incubated only with water. Each assay was performed three times. After 6 days the germination inhibition of each species was expressed as percentage of the respective control. Replicate errors of three samples were below 6% in all cases. Data were analyzed by one-way ANOVA. Seedling fresh biomass weights were obtained at the 6 days from the germination of seeds as average percentage of the control.

The capacity factors (k') for the compounds used in this study were determined by

k ′ = ( t R − t M ) / t M

where t_R is the retention time of the compound and t_M is the retention time of non-retained compound (thiourea). Reverse phase high performance liquid chromatography (RP-HPLC), analyses were carried out in C₁₈ columns with mobile phase water (pH = 3.0, phosphoric acid)/acetonitrile 60:40 v/v.

The relationship between k' and the n-octanol-water partition coefficient was established by linear regression of logk' and log kow values obtained from the literature. Predictive logP_HPLC values were then derived from the equation:

log P HPLC = 1.034 log k ′ + 2.176

Indole: 5-carbaldehyde índole, carbomethoxy índole, 5-hydroxy índole, 5-carboxylic acidíndole , 5-methanol índole, 5-cyano índole, 5-bromo índole were purchased from Aldrich Chemical Co. All solvents were HPLC grade. Water was obtained from Milli-Q purification system (Millipore USA).

Green microalgae, such as Chlorella, are taxonomically classified as plants bearing some similarity to higher plants and for this reason the microalga test may be used to evaluate herbicidal activity against higher plants. Anti-algal activity of the indole series (Figure 1) was measured using the fresh water green alga C. vulgaris. Percentages of in vitro growth inhibition are shown in Table 1. To provide comparison of anti-algal activity, previously reported data for gramine [11] were used as a reference.

In the concentration range studied (from 12.5 µg∙mL⁻¹ to 100 µg∙mL⁻¹) all the test compounds, with the exception of 5-methanol and 5-carboxylic indole acid, showed higher levels of toxicity than gramine.

At a lower dose (12.5 µg∙mL⁻¹) the percentages are more diverse and allow the evaluation of substituents in the aromatic ring with more clarity. 5-bromo and 5-carbomethoxy indoles showed the highest antialgal activity, the 5-cyano and 5-hydroxy indoles displayed less activity and the 5-methanol indole and 5-carboxylic indole acid showed no activity. In addition, 5-carboxylic indole acid stimulated the growth of C. vulgaris; the increase in biomass with respect to control was proportional to the concentration in the range from 25 to 500 µg∙mL⁻¹ (2.3% to 15.7%).

Part of the phytotoxicity of indole alkaloids (Figure 1) has been related to the structural changes of the side chain bonded at the 3-position [11] . Now, our results suggest strongly that the substituent in the aromatic ring could play an important role in the anti-algal activity.

Cellular Ca²⁺ efflux in algae has been proposed as one of the possible mechanisms that inhibits the growth of the algae Cylindrotheca sp and other bioactivity of indole and gramine [22] . Their results showed that gramine had more anti-algal activity than indoles substituted with halogen atoms in the aromatic ring.

Our results do not coincide with the findings of Yang et al. [22] , because the 5-bromo indole showed more anti-algal activity that gramine. Therefore, further research is necessary to fully clarify the anti-algal mechanism of these compounds.

The phytotoxicity of the compounds involved in allelopathic effects depends upon the dose and target species. For this reason, phytotoxicity of the indole derivatives was also examined on seed germination for five species of cereals and one weed.

Table 2 shows the effect of an aqueous solution with two different concentrations of 8 indole derivatives and gramine on seeds germination for wheat (T. durum), oat (A. sativa), rye (S. cereale), barley (H. vulgare L), maize (Z. mays L) and the weed Lollium rigidum.

The germination inhibition indicator (I%) showed diverse values for the competitive species of cereals and the weed, which allowed an evaluation of the selective phytotoxicity of the indole derivatives with respect to the selectivity of the gramine alkaloid.

The weed L. rigidum, a small-seeded-species, was the most inhibited at the two concentrations used of 5-bromo, 5-carbomethoxy and 5-cyano indole derivatives with respect to gramine. The maize species, a large-seeded-species, was the most tolerant toward all compounds.

Gramine is the main indole alkaloid from barley that might be involved in the allelopathic property of this cereal. The phytotoxicity of indole is particularly relevant to understanding the allelopathy of this compound; because as is mentioned above this unsubstituted indole structure can be produced by the decomposition of gramine in the soil. Hence the results suggest that the allelopathic activity of barley on cereals and weeds might be due to the phytotoxic effect of gramine¹¹ and the indole residue. Moreover, our results suggest that barley is not affected by autotoxicity from gramine, but this may be due to indole according the inhibitory effects showed by this compound (Table 2).

The effect of water-soluble inhibitor compounds associated with allelopathy is often more pronounced on plant growth than on its germination and depends on the dose and the receptor plant. Thus fresh seedling weight may be also a bioindicator to evaluate the phytotoxicity of chemicals. In consequence, we examined the toxic effect of the indole derivatives on the growth of these five species and the one weed. Table 3 shows the effect of aqueous solutions of 250 µg∙mL⁻¹ concentration of the eight indole compounds on the seedling fresh weight of those that germinated respect to the control.

As is observed, the seedling biomass weight showed diverse values of inhibitory and stimulating effects on the growth of the species and the weed. Indole, 5-carbomethoxy, 5-bromo and 5-cyano derivatives had the greatest inhibition effect (I%) on the growth of all the species. The average percentages were 46%, 77.8%, 59% and 85%, respectively. These results are similar to their effects inhibiting germination and anti-algal activity. Gramine, 5-carbaldehyde and 5-carboxylic indole acid showed an average inhibition percentage less than 25%. 5-methanol indole did not seem to inhibit germination, moreover, it stimulated (+%I values) the growth of all species, except rye. Similarly, 5-hydroxyindole showed stimulation of Lollium rigidum and wheat.

These results show that the selectivity phytotoxic effect depends on the receptor species and the substitution in the aromatic ring. Although the concentrations used in this study are probably greater in the field, they are within gramine is found in plants (0.2 - 7.0 mmol/kg fr.wt.) [23] [24] [25] . Therefore, these results suggest that indole derivatives release in the soil from of natural indole alkaloids or other sources may be involved in the allelopathic phenomenon of plants.

Nd = not determined; Ng = no germinated seeds.

Phytotoxicity in plants may have some of the following effects: toxicity to the radicle growth of monocots and dicots; inhibitory effect on the energy metabolism of chloroplasts and mitochondria; modification of the binding affinity of the receptor sites of membranes.

The chemical mechanism of the phytotoxic activity of indoles is not well understood. As mentioned above, part of the effect depends on the structure of the side chain, in the natural indole alkaloids [11] . Our results suggest that the activity could be also controlled by the substituent in the aromatic ring. Parameters such as lipophilia and electronic character of the substituent can be involved in the molecular mechanism of action or the dynamic in the environment.

The effect of electronic properties of the aromatic substituent in indole structure has been previously analyzed using the molecular orbital theory [26] . An equation predicting phytotoxicity against the germination of Lettuce (Lactuca sativa) from HOMO-LUMO frontier orbitals was obtained. From this equation the structure-activity relationship predicted that electron-acceptor substituents increase activity because they have a profound effect on the strength of the N-H bond in the heterocyclic ring, favoring possible H-bond formation with suitable groups at the active site. A structure-activity study on similar structure activity such as 5-benzoxazolinones, hydroxamic acids and acetanilide, suggests that the electron-acceptor effect of the substituents also plays an important role in on the polarization of N-H bond and the biological activity [27] [28] [29] .

From our results, additional specific evidence in the indole structures is obtained by comparing the σ_p values of the substituents, a measure of the electronic properties of the aromatic ring substituent [30] . The substituted derivatives with the electron-acceptor groups 5-carbomethoxy (σ_p = 0.44), 5-cyano (σ_p = 0.71) and 5-bromo (σ_p = 0.26) showed more activity than the derivative with an electron-donor group, 5-hydroxy (σ_p = −0.38), the unsubstituted indole (σ_H = 0.0) and gramine.

However, the different activities observed between the 5-carbomethoxy (σ_p = 0.44), 5-carbaldehyde (σ_p = 0.47) and 5-carboxylic acid (σ_p = 0.44) and those observed between indole (σ_p = 0.0) and 5-methanol (σ_p = 0.01) derivatives may not be associated with the electronic properties of these substituents, because they have similar σ_p values.

Lipophilicity is an essential parameter to establish the quantitative structure-activity relationship of phytotoxicity. The lipophilic-hydrophilic balance may have a fundamental role in the penetration into seeds and dynamics of the physiological system [31] [32] [33] . The n-octanol/water partition coefficient (kow), determined by the traditional shake-flash method, is the parameter most commonly reported as a measure of the lipophilic character of chemicals, although frequently an insufficient kow data set is a problem in structure-activity relationship studies. The correlation between the capacity factor (k') obtained from RP-HPLC methods and kow is generally accepted for simple and rapid estimation of partition coefficients [34] [35] [36] [37] . With this in mind, we established RP-HPLC as alternative method to evaluate the lipophilicity of the indole series. A linear regression between the capacity factor and log kow from literature sources for some compounds was obtained (R² = 0.895). From this regression logP_HPLC values were derived by a single equation (see experimental). Table 3 gives the logP_HPLC values. The role of lipophilicity in the phytotoxic effect of the series studied can be analyzed from these values. In general the compounds with the highest logP_HPLC values showed the highest percentages of inhibition effect on seeds germination and biomass growth. Through the different target species are affected on the biomass very differently from each other, more clarity about the lipophilic effect on the biomass growth can be obtained from correlation between the average inhibition percentages and the logP_HPLC values include in Table 2. As shown in Figure 2 the inhibition percentages increase linearly with the lipophilic character of the compounds. The carbomethoxy and CN derivatives display inhibition percentages higher that predicted. This deviation could be ascribed to the electron-acceptor component of these groups. In the other cases the lipophilic properties should be prevailing.