This study was based on a laboratory research design, because the study focused on investigating the drainage wastewater quality analysis and its treatment. At first, a field visit was carried out in various drainage outlets of KCC, mainly the Daulatpur to Gollamari bazar areas. Then the methods outlined in the Standard Methods for the Examination of water and wastewater [12] were followed for the analyses of collected wastewater samples for all the physical and chemical parameters. TDS and TSS were determined by using filter paper and oven. After filtering the sample by the filter paper, it was placed into oven at 105˚C for 24 hours. Conductivity meter was used for the determination of electrical conductivity and pH meter (HACH, sension 2) was used for determination of pH. Turbidity and color were measured with a Partech DRT 100B Turbidity meter and a color comparator respectively. For the determination of sulphate (SO₄) and Nitrate (NO₃), Sulfa-Ver 4 reagent and Nitra-Ver 5 reagent were used. EDTA was used in burette for titration in hardness test and Eri-chrom black T (EDT) was used as the reagent and titrated until the blue colour formed. DO bottle and DO meter (HACH, HQ 40d) were required for laboratory analysis of BOD₅ and 5 days were needed for the test. For determination of COD, K₂Cr₂O₇ was taken in the pipette and ferrion indicator was used as reagent and titrated until the radish color formed. Total coliform and faecal coliform were measured by the membrane filtration technique by using XMG Agar reagent into a petri-dish. Therefore, a laboratory-scale wastewater treatment plant was developed and treatment efficiency of the plant was monitored. Finally, paying attention to effluent quality of laboratory-scale treatment plant, modification has been made for better performance.

There are mainly ten outlets from which the wastewater releases into the Mayur River. The information regarding the drain outlets into the Mayur River and sampling locations are shown in Figure 1. Among 10 drain outlets, wastewater samples were collected from drain outlet 2 (Goalkhali, Bastuhara colony), 3 (Boyra Shahanghat bridge) and 7 (Gallamari, Gallamari bridge) for raw wastewater quality testing during December, January and April respectively. In addition, around Khulna city, during December to May farmers are facing freshwater scarcity for irrigation purpose [13] . However, the wastewater samples were collected from 50 to 100 feet ahead of drain outfall. The collected wastewater samples were analyzed individually. Drain outlet-2 and outlet-3 get water from almost domestic wastewater because this outlet passes through residential area including huge slaughter houses and there was no industrial and activity around outlet-2 and outlet-3. Moreover, this area has some clinics and hospitals and plastic recycling factories. Drain outlet-7 carries wastewater from residential areas, some factories and markets as well. This wastewater is also dumped directly into open channels after generation. Because of these highly contaminated domestic wastewaters, market wastewater and factory related wastewater of these three-drain outlets were considered as sampling points for this study. Table 1 illustrates details about sampling station including drain outlets, sample ID and location.

The KCC drain outlets were the potential sources of drainage wastewater sample collection of the study. Three drain outlets (No. 2, 3 and 7) were taken with random sampling from total ten drain outlets, mentioned in Table 1 and Figure 1, as raw wastewater sampling stations. From every sampling station, total three samples were collected with special care. In December 2015, one wastewater sample from each sampling station was collected for laboratory testing and same procedures were followed for month of January and April 2016. New plastic “Jerry Can” of 20 litter capacity with hard plastic screw cap was used for wastewater sample collection. The can was properly cleaned before using and washed 2 - 3 times before sampling. Wastewater samples were collected from the midpoint of the trunk drains by dipping each sample Jerry Can approximately 15 - 20 cm below the water surface, opening the Jerry Can, allowing it to fill in, then closing each Jerry Can with its cap under water. Wastewater samples were collected and transported to the laboratory on the same day. The samples were then preserved in a refrigerator at about 4˚C until analysis. In all analyses samples were measured at dry weather conditions.

Wastewater treatment consists of applying known technology to improve or upgrade the quality of wastewater. This study follows collecting the wastewater samples and subjecting the wastewater to various treatment processes, as discussed below:

1) To identify the treatment units needed for the development of laboratory-scale treatment plant, the physical, chemical and biological characteristics of wastewater were investigated in the laboratory. After the laboratory tests of DO, BOD₅, COD, TSS, TDS, NO₃, SO₄, pH, Conductivity, Color, Turbidity, and Coliforms of raw wastewater samples (three samples from each sites) and analyze secondary data, the design criteria were set for the treatment units. Based on the developed criteria, a laboratory-scale drainage wastewater treatment plant was constructed in the laboratory (Figure 2).

2) In this research work, a bar screen was used which consists of 5 mm × 5 mm steel wire mess to cover up the rectangular primary sedimentation tank. The incoming wastewater passed through the bars or screens and the accumulated material was removed manually before colloguing the screen.

3) For laboratory-scale study, a 45-litter capacity plastic rectangular container was used as primary clarifier. The size of the container was 20 inches in length, 15 inches in wide and 10 inches in height with 2-inch free board (Figure 2(a)). The detention time was fixed to 2 hours for the sedimentation of settle able materials. In every batch of treatment, 40 liters of wastewater sample entered the chamber for 2 hours, and a path was made from the bottom of the rectangular tank to remove sludge that accumulated in this stage of treatment.

4) A 20’’ × 15’’ × 10’’ size plastic tank (Figure 2(b)) was used as an aeration chamber and a mechanical device (Air blower: SP-780 model Super Pump) was used to support a continuous source of oxygen. The chamber also made with a 2-inch freeboard for preventing overflow. Aeration to activate microorganism, was done for 24 hours with 2 hours detention time. After the completion of aeration, an optimum alum dose of 70 mg/L was added for chemical precipitation after being determined in the laboratory. Manual steering was done for complete mixing with 30 minutes intervals for 2 hours, and then sample was kept at rest for another 2 hours for settling down of sludge. Sludge was collected from the bottom of the chamber through gate valve that was incorporated for regulation of flow.

5) Chemical precipitation and granular filtration incorporated for phosphorous and nutrient removal purpose. Up-flow roughing filter retains the floc generated from chemical precipitation. After roughing filtration, the effluents passed through sand filter for nitrogen and pathogenic bacteria removal. Roughing filter and sand filter were made with a 10’’ × 15’’ × 10’’ sized (Figure 2(c) and Figure 2(d)) chamber full of brick khoa (crushed brick) and Sylhet sand. The brick khoa used for roughing filtration was 2 mm to 40 mm in size. Similarly, the effective size of sand for sand filtration was 0.45 mm to 1.5 mm. The chambers also consisted with a 2-inch freeboard for preventing overflow.

6) The collected raw wastewater sent into the sedimentation tank, which passed through a connecting hose pipe into the aeration tank. The wastewater

was aerated for 24 hours before pouring the effluent into filter media so that oxygen can be mixed with wastewater properly. After aeration, an optimum alum dose (70 mg/l) put into aeration chamber to remove phosphorous. Then water passed through the up-flow roughing filter to catch the floc generated in chemical precipitation. After this, water again transferred into the sand filtration unit for nitrogen and bacteriological removal. Finally, a storage tank used to store treated effluent for chlorination before discharging. No power was used for the flow generation. Gravitational force controlled whole flow system. The entire setup is presented in Figure 2.

The results of different drain outlet’s raw wastewater samples revealed the large number of polluting agents that are dumped every day in bodies of water without any treatment, which is alarming for the ecology of inland surface water. The chloride is the most dominant anion in wastewater generated in Khulna city irrespective of sampling station. The least dominant element is iron in the study area. The test results of raw drainage wastewater are presented in Table 2, which

shows a wide range of variation of drainage wastewater quality from different sampling stations of drain outlets of KCC. The values of raw wastewater parameter (SS-1, SS-2 and SS-3) presented are the average of three months (i.e. December, January and April) in each sampling station.

Performance of a developed laboratory-scale treatment plant (Table 3) varied depending on the parameters for which the plant was designed. Some parameters improved and satisfied the standard limit, whereas others did not. The removal of BOD₅, COD and TDS were not very good, because aeration alone is not sufficient for their reduction. Maximum 53% COD and 52% BOD₅ reduction were obtained for 24 hours aeration. Similarly, 24.7% and 60.0% reduction were attained for TDS and TSS respectively from 24 hours of aeration process. The application of alum and granular filter media accounted for a 90% and 96% reduction for fecal coliform and total coliform, respectively. Phosphate and nitrate reduction rate were 97% and 98.0%, respectively, after treatment with the developed laboratory-scale treatment plant. Table 3 clearly stated that BOD₅, COD, and coliforms do not meet ECR’97 standard. So, the developed laboratory-scale

*ECR’97: The Environmental Conservation Rules (1997) [14] .

treatment plant needs to be modified for better BOD₅, COD, and Coliforms reduction efficiency. The values of effluent parameter (SS-1, SS-2 and SS-3) presented are the average of three months (i.e. December, January and April) in each sampling station.

The laboratory-scale treatment plant could not remove all harmful contaminants from the wastewater as per standard requirement of ECR (1997). The modification of laboratory-scale treatment plant was made by activated sludge processing following filtration. The activated sludge process unit consisted of a high-quality plastic container as the primary clarifier, a reactor for the aeration chamber with an air blower, and a secondary clarifier with a pump for returning sludge. Three, five and seven days mean cell residence times were considered to activate the microorganisms in the activated sludge process unit. The effluents were passed through a granular filter media made from stone chips and sand (Figure 3). The filter media was constructed with locally available stone chips bed at the bottom of the container, along with a sand bed on its top. Both granular materials were well cleaned before use and each formed a uniform thick layer of 4-inch height. The bottoms of the filter chamber were kept empty for 2 inches for collection of sludge.

The effluent from the secondary clarifier traveled through the stone chips bed, followed by the sand bed, forming an up-flow of wastewater. The influent was

passed through the screen (5 mm × 5 mm) to strain off any coarse solids into the primary clarifier, which was also worked as a grit removal chamber. Then it was discharged to the reactor, an aeration chamber with a flow rate of 10 ml/min. The hydraulic retention time in the aeration tank for this developed treatment unit was calculated as 4 hours. The wastewater was then transferred to the secondary clarifier for bacterial floc formation and settlement as active sludge. A return sludge line from the secondary clarifier was linked through a pump, which returned 5 ml/min. of activated sludge to the reactor. A balance was made in the wastewater flow rate between the reactor and secondary clarifier. Another sludge line was formed in the secondary clarifier to discard a fixed amount of wastewater, with flow rate 0.05 ml/min. to maintain the activated sludge process properly.

After the continuation of all these steps, the treated wastewater passed through a final filter media. Following activated sludge processing in a slow sand filter, the operating filtration rate varied between 0.1 - 0.3 m³/m²/hr. The top layer of the sand becomes biologically active by the establishment of a microbial community on the top layer of the sand substrate. After treatment with an activated sludge process, the effluent needed to pass slowly through the coarse stone media and sand filter to improve the water quality. Instead of following the traditional down-flow method, the flow path was made upward through the stone chips bed layer and then sand bed. Finally, the effluents were disposed of through an outlet arrangement. This approach is recommended for longer filter runs with less operation and maintenance requirements [15] .

The performance of the laboratory-scale treatment plant after modification was monitored closely to determine the treatment efficiency of this system. The raw wastewater was collected from sampling station III. The test results from the raw wastewater and effluents from modified laboratory-scale treatment plant are illustrated in Table 4.

1) Biochemical Oxygen Demand (BOD₅)

BOD₅ is the most popular criteria parameter for organic pollution, useful to both wastewater and surface water. The raw wastewater sample has a BOD₅ value of 226 mg/l, which represents a lot of organic content present in the wastewater. After treatment of 3-days, 5-days and 7-days BOD₅ levels decreased to 9.8 mg/l, 1.78 mg/l and 1.38 mg/l, respectively. This shows that for 5 and 7 days, BOD₅ values fulfill the ECR (1997) standard limit of 50 mg/l, but 3 days does not in the range (Table 4). Based on test result, it can be concluded that moderate concentration of organic matter is decomposed when wastewater kept more than 5 days into secondary settling tank.

2) Chemical Oxygen Demand (COD)

The COD test measures the organic matter present in industrial and municipal wastewater while BOD₅ describes amount of oxygen required to decompose organic matter present in wastewater. The COD value of raw wastewater was 435 mg/l whereas after of 3-days, 5-days and 7-days, the values were measured at 192 mg/l, 96 mg/l and 32 mg/l, respectively. The standard value of COD for industrial wastewater for disposal into surface water bodies is 200 mg/l (ECR, 97), which was fulfilled in all three cases. Calculation shows about 56%, 78% and 93% removal efficiency measured from biological treatment of 3-days, 5-days and 7-days, respectively.

3) Total Dissolved Solids (TDS)

TDS is important to be considered in the calculation of irrigation water quality, because many of the toxic solid materials may be imbedded in the wastewater, which may cause harm to the plants [16] . Table 4 illustrates the final TDS values reported after treatment were 1667 mg/l, 705 mg/l and 590 mg/l for 3-days, 5-days and 7-days, respectively, which shows a positive trend of treatment of wastewater by the activated sludge system, whereas for raw wastewater the value was 2525 mg/l. Thus, the average effluent concentrations were below the ECR (1997) standard limit (2100 mg/l).

4) Total Suspended Solids (TSS)

Suspended solids refer to small solid particles which remain in suspension in water as a colloid or due to the motion of the water. It is used as one indicator of water quality. The raw wastewater sample had a TSS concentration 190 mg/l. After treatment, TSS levels in the final effluents decreased to 95 mg/l, 45 mg/l and 35 mg/l for 3-days, 5-days and 7-days, respectively. The standard limit of TSS concentration for disposal into inland water bodies is 150 mg/l (ECR, 1997). Thus, Table 4 shows that after treatment, the TSS concentration of effluents lie in the range of the standard limit.

Table 5 illustrates that 49% BOD₅ reduction observed from laboratory-scale treatment plant, but after modification, the reduction efficiencies improved to 96%, 99% and 99% for 3-days, 5-days and 7-days, which also satisfies the ECR (1997) standard limit. COD and TDS reduction efficiencies are 41% and 41% for the primarily developed laboratory-scale treatment plant, while 56%, 78% and 93% COD and 34%, 72% and 77% TDS reduction efficiencies were achieved from the modified treatment plant for 3-days, 5-days and 7-days, respectively.

The result shows a positive trend of treatment efficiency of the wastewater treatment by the modified laboratory-scale treatment plant.

A large change was also noticed with solids after treatment in the laboratory-scale treatment plant after modification, specifically in TSS removal. In the case of the primarily developed treatment plant, the TSS removal efficiency was 55%, whereas for the modified plant it rises to 82% for 7-days treatment. Based on the findings, it can be summarized that, the laboratory-scale treatment plant after modification is more efficient than primarily developed treatment plant.

Effluents from wastewater treatment plants (WWTP) are widely used in different industries e.g. agriculture, cooling towers, etc., and can enter directly into the ecosystem through discharging to surface or groundwater [17] . The discharge of wastewater from municipal, industrial, and agricultural areas is an issue of serious concern as it affects a river’s ecology [18] . These widespread usages of treated wastewater compel legislators to set stringent rules and regulations with respect to WWTP effluents.

This study reveals that the effluent quality stated in Table 6 for discharging into surface water bodies mostly satisfies the standards. On the other hand, FAO standard for TDS (450 - 2000 mg/l) and TSS (50 - 100 mg/l) are also satisfy after

*Data taken from effluent of sampling station III.

*Effluents form modified laboratory-scale treatment plant.

treatment by modified laboratory-scale treatment plant. The BOD₅ of raw wastewater is high because the organic content in the city wastewater is usually high. After biological treatment for 7 days in the activated sludge processing unit following filtration, the BOD₅ was reduced drastically to 1.38 mg/l, which satisfies FAO water quality standards that are highly suitable for irrigation.