<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">OALibJ</journal-id><journal-title-group><journal-title>Open Access Library Journal</journal-title></journal-title-group><issn pub-type="epub">2333-9705</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/oalib.1104297</article-id><article-id pub-id-type="publisher-id">OALibJ-85984</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biomedical&amp;Life Sciences</subject><subject> Business&amp;Economics</subject><subject> Chemistry&amp;Materials Science</subject><subject> Computer Science&amp;Communications</subject><subject> Earth&amp;Environmental Sciences</subject><subject> Engineering</subject><subject> Medicine&amp;Healthcare</subject><subject> Physics&amp;Mathematics</subject><subject> Social Sciences&amp;Humanities</subject></subj-group></article-categories><title-group><article-title>
 
 
  The Impacts of Crude Oil Exploitation on Soil in Some Parts of Ogoni Region, Rivers State, Southern Nigeria
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Inah</surname><given-names>Eteng Okon</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Chima</surname><given-names>Okoko Ogba</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib></contrib-group><aff id="aff2"><addr-line>Geoid and Environmental Services Ltd, Port Harcourt, Nigeria</addr-line></aff><aff id="aff1"><addr-line>Geography and Environmental Science, University of Calabar, Calabar, Nigeria</addr-line></aff><pub-date pub-type="epub"><day>05</day><month>07</month><year>2018</year></pub-date><volume>05</volume><issue>07</issue><fpage>1</fpage><lpage>20</lpage><history><date date-type="received"><day>29,</day>	<month>December</month>	<year>2017</year></date><date date-type="rev-recd"><day>13,</day>	<month>July</month>	<year>2018</year>	</date><date date-type="accepted"><day>16,</day>	<month>July</month>	<year>2018</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
  The study examined the impacts of crude oil exploitation on the soil environment of Ogoni region and also identified action plans for the future management of the region. Composite soil samples were collected at two depths: 0 - 15 cm (surface soils) and 15 - 30 cm (subsurface soils) along different positions of landscape in the four study locations. Samples were analysed in the laboratory within 5 days of collection. ANOVA was used to confirm that both soil chemical and physical properties significantly vary in the study locations. The result revealed that sand, silt and clay contents significantly vary in Eleme, Tai, Khana and Gokhana as evident in the calculated F-ratios of 307.70; 606.72; 1312.31; and 1154.02 respectively. This is against the tabulated F-ratio of 3.35 as reflected by the wide variations in sand, silt and cla
  y fractions, and probably owing to differences in parent material. Based on the locations where the soils were sampled, the results indicate that the soils vary widely in chemical characteristics in Eleme (F-ratio = 1674.16; p &lt; 0.01; F-critical = 1.88); Tai (F-ratio = 82.67; p &lt; 0.01; F-critical = 1.88); Khana (F-ratio = 1467.66; p &lt; 0.01; F-critical = 1.88) and Gokhana (F-ratio = 709.49; p &lt; 0.01; F-critical = 1.88) against the theoretical value. The soils of the prescribed study area are declared contaminated by heavy metals and hydrocarbon toxicity. The study therefore recommended the immediate implementation of the UNEP Report on Ogoni to attempt a remediation of the pollution impacts on the environment and socioeconomic livelihood. The paper also recommends the regulation of the activities of multinational oil companies in mineral exploration in Nigeria.
 
</p></abstract><kwd-group><kwd>Crude Oil Exploitation</kwd><kwd> Soil Pollution</kwd><kwd> Physico-Chemical Properties</kwd><kwd> Oil Spillage</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>Oil production in Nigeria has severe environmental and human consequences on the indigenous people who inhabit such areas. Nigeria’s export of 2.2 million barrels of oil a day comes from 12% of the country’s land [<xref ref-type="bibr" rid="scirp.85984-ref1">1</xref>] yet indigenous minority communities in these areas receive little economic benefits. Development strategies that are focused on increasing foreign investments in Nigeria’s oil industry as to boost exports have not caused overall development. The revenue gained has helped to benefit foreign nations and the Nigerian government elite more than the native populations. Indigenous groups are actually impoverished due to environmental degradation from oil production and the lack of adequate regulations on multinational operations thus making the local communities more vulnerable to environmental problems such as food shortages, health hazards, loss of land, pollution, forced migration and unemployment. The affected groups include the Andonis, Edos, Efiks, Ibibios, Ijaws, Ika-Ibos, Ikwerres, Isekiris, Isokus, Kalabaris, Urhobos and Ogonis, whose approximate 36 million people make up one fourth of Nigeria’s population. Given that 90% of the Nigeria’s total national revenue comes from oil production, and the tendencies of oil companies to maximize profits, both institutions have an interest in maintaining production at the status quo [<xref ref-type="bibr" rid="scirp.85984-ref1">1</xref>].</p><p>The history of oil exploitation in Ogoni runs parallel with the history of oil pollution as the commencement of oil exploration and exploitation runs concomitantly with the three major causes of oil pollution namely, the impact of the seismic survey, gas flaring and oil spills [<xref ref-type="bibr" rid="scirp.85984-ref2">2</xref>]. The Ogonis have sought more political autonomy and compensation for environmental damage to their land by oil companies and this has been met with negligence or brutal force. The consequences of oil exploitation on the environment of this region are what informed the need for this study whose objectives include among others to examine the impact of crude oil exploitation on Ogoni soils.</p><p>Oil industry activities are inevitably associated with environmental pollution. The major Nigeria’s oil region lies exclusively within the Niger Delta and its continental shelf, which is saddled with most of the industry’s oil installations and activities (upstream and downstream) and their associated deleterious environmental impacts. The industry operates over a thousand oil producing wells, gas plants; network of thousand kilometres of pipelines (Right of Ways) criss-crossing the Delta bearing crude oil to flow stations, terminals, and refineries. On average, one oil spill occurs every week in Nigeria. Pipelines are laid across farms, waterways and fishing grounds. Some pipes cross communities and living quarters. Approximately, 6000 km of pipelines cover Ogoni land [<xref ref-type="bibr" rid="scirp.85984-ref3">3</xref>].</p><p>However, due to incessant oil spills, oil has coated the aerial roots of plants killing parts of the mangrove forest and its faunal dependence. This mangrove forest, which serves as habitat for fish and molluscs as well as a source of raw materials for communities in Ogoni, has been ravaged by oil pollution. The land, the sea and the air (which they have depended on for several thousand years) can no longer support the subsistent life of the Ogoni community. Typical of this example is the abundant mangrove vegetation in Ogoni community of Bodo where the livelihood of the local people was previously sustained through farming and fishing. They also gathered mangrove wood for building and for local energy and fuel. Today, most of the youths and women have become jobless since their local economic support system is no longer sustainable. Gas had been flared for 24 hours daily for 40 years in close proximity to human habitation in nineteen oil locations [<xref ref-type="bibr" rid="scirp.85984-ref4">4</xref>]. This has been done without regard to the negative impacts of such activities on the people and the environment.</p></sec><sec id="s2"><title>2. Geographical Description of the Study Area</title><p>The Ogonis are indigenous ethnic minority group in Rivers State in the Niger Delta region of Nigeria (<xref ref-type="fig" rid="fig1">Figure 1</xref>). Ogoni has an area of 1046.4 square km, which lies between longitude 7˚2'00&quot;E and 7˚18'30&quot;E and latitude 4˚18'30&quot;N and</p><p>4˚31'00&quot;N. Ogoni is bounded in the north by Oyigbo LGA; west by Okrika LGA; south by Ogu/Bolo, Bonny, Andoni and Opobo/Nkoro LGAs; and in the east by Akwa Ibom State, naturally separated by Imo River. The Ogoni people live in the coastal plain terraces northeast of the Niger Delta. Ogoni region is divided into four Local Government Areas, viz: Tai, Eleme, Gokana and Khana, and into six regions/clans in the Delta: Ken - Khana, Baabe, Bori, Tai, Gokana, and Eleme. The region, located within the coastal and rainforest belt, is characterized by mangrove swamp forests and rich rainforest vegetation that the Ogoni people depend on [<xref ref-type="bibr" rid="scirp.85984-ref5">5</xref>].</p><p>The Ogoni people are predominantly engaged in farming and fishing for subsistence. They also engage in tapping and distillation of palm wine into a local brewed dry gin, boat building, mat and pottery making. The people also earn their living as civil servants and traders [<xref ref-type="bibr" rid="scirp.85984-ref6">6</xref>]. Before the era of oil production in the late 1950’s, Ogonis cultivated several crops ranging from banana to sugar cane. Oil was discovered in the Ogoni territory in early 1958 when SPDC found oil in the Ogoni community of K-Dere popularly and misnomally called the Bomu oil fields [<xref ref-type="bibr" rid="scirp.85984-ref2">2</xref>]. Subsequently, Shell made more discoveries in other Ogoni communities including Ebubu, Yorla, Bodo West and Korokoro. On the average, Ogoni had five major oil fields with 110 oil wells, hooked up to five flow stations at Bomu, Korokoro, Yorla, Bodo West and Ebubu by a necklace of interconnecting pipelines which criss-crossed Ogoni villages [<xref ref-type="bibr" rid="scirp.85984-ref2">2</xref>]. Ogonis have a population of 831,726 people and reside in the northeast area of the Delta. It is made up of four Local Government Areas including: Tai with a population of 117,797 people; Eleme comprising of 190,884 people; Gokana with 228,828 people; and Khana with apopulation of 294,217 [<xref ref-type="bibr" rid="scirp.85984-ref6">6</xref>]. The region’s population is however estimated at 1,066,393 by 2015, using the 2.8% national annual growth rate.</p></sec><sec id="s3"><title>3. Methods and Sampling Techniques</title><p>The practical problem faced by this research is the large size of the study area. In this regard therefore, a total of twenty communities were chosen for easy comprehension of the research problem. Composite soil samples were collected at two depths: 0 - 15 cm (surface soils) and 15 - 30 cm (subsurface soils) along different locations of the landscape in the four study locations spanning Khana (Bono Ogoi, Okloma, Bolem, Kporghor and Sim luekon); Gokana (K-Dere, Biara, Yeghe, Barako and Kiani); Eleme (Akpajo, Ejaka, Ogali, Agbonchia and Onne); and Tai Local Government Areas (Okwale, Luuku, Kpang, Bere and Kani) within the Ogoni region, Rivers State. These communities were systematically chosen to represent local government headquarters, areas of major oil production facility and areas without an oil production facility.</p><p>The soil samples collected were air-dried (room temperature), ground with wooden roller and sieved via 2 mm mesh. Particle size distribution was determined by Bouyoucos hydrometer method [<xref ref-type="bibr" rid="scirp.85984-ref7">7</xref>] using sodium hexa-metaphosphate as a dispersant and the textural classes determined using the textural triangle chart.</p><p>Soil pH was determined using the method of IITA [<xref ref-type="bibr" rid="scirp.85984-ref8">8</xref>]. The method of [<xref ref-type="bibr" rid="scirp.85984-ref9">9</xref>] was used in the determination of organic carbon. Available phosphorus was determined using [<xref ref-type="bibr" rid="scirp.85984-ref10">10</xref>] No. 1 method. Total nitrogen was determined by the micro-Kjeldahl digestion method. Exchangeable bases (Ca, Mg K and Na) were extracted with neutral IM NH4 OAc, pH 7.0; the potassium and sodium in the extract was by flame photometry while calcium and magnesium was by Versenate EDTA titration method [<xref ref-type="bibr" rid="scirp.85984-ref8">8</xref>]. Cation exchange capacity (CEC) was obtained by the summation of exchangeable bases. Heavy metal contents of the soils were extracted by digestion of the samples with a mixture of concentrated HN03 and HCl and their concentrations determined by Atomic Absorption Spectrophotometry (AAS) using “Buck Scientific 200A” by flame atomization [<xref ref-type="bibr" rid="scirp.85984-ref11">11</xref>]. Total hydrocarbon (THC) was determined by extracting the soil with carbon tetrachloride and measuring the total hydrocarbon content calorimetrically at 420 nm using spectronic 20 (<xref ref-type="table" rid="table1">Table 1</xref>).</p></sec><sec id="s4"><title>4. Hypotheses</title><p>1) H0: Soil physical properties do not significantly vary in the study locations.</p><p>H1: Soil physical properties significantly vary in the study locations.</p><p>2) H0: Soil chemical properties do not significantly vary in the study locations.</p><table-wrap id="table1" ><label><xref ref-type="table" rid="table1">Table 1</xref></label><caption><title> Methods and equipment for physico-chemical analyses</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Analytical equipment/method and reference</th><th align="center" valign="middle" >Parameter (s)</th></tr></thead><tr><td align="center" valign="middle" >Cyberscan pH 20 meter</td><td align="center" valign="middle" >PH, Eh</td></tr><tr><td align="center" valign="middle" >Cyberscan low 20 conductivity meter</td><td align="center" valign="middle" >Temperature, conductivity, TDS</td></tr><tr><td align="center" valign="middle" >Microprocessor Oximeter 196</td><td align="center" valign="middle" >Dissolved Oxygen (DO)</td></tr><tr><td align="center" valign="middle" >Atomic Absorption Spectrophotometer (ASS)</td><td align="center" valign="middle" >K, Na, Heavy metals</td></tr><tr><td align="center" valign="middle" >Spectrophotometrically by:</td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >(a) Turbidimetry using barium chloride [<xref ref-type="bibr" rid="scirp.85984-ref12">12</xref>]</td><td align="center" valign="middle" >Sulphate, SO 4 2 −</td></tr><tr><td align="center" valign="middle" >(b) Diazotisation method [<xref ref-type="bibr" rid="scirp.85984-ref13">13</xref>]</td><td align="center" valign="middle" >Nitrite (NO<sub>2</sub>)</td></tr><tr><td align="center" valign="middle" >(c) As nitrite after reduction in a calcium reduction system [<xref ref-type="bibr" rid="scirp.85984-ref13">13</xref>]</td><td align="center" valign="middle" >Nitrite (NO<sub>3</sub>)</td></tr><tr><td align="center" valign="middle" >(d) Molybdenum blue method [<xref ref-type="bibr" rid="scirp.85984-ref13">13</xref>] .</td><td align="center" valign="middle" >Phosphate ( PO 4 3 − )</td></tr><tr><td align="center" valign="middle" >(e) Formazine standards according to HACH</td><td align="center" valign="middle" >Turbidity (NTU)</td></tr><tr><td align="center" valign="middle" >(f) Nesslerization method [<xref ref-type="bibr" rid="scirp.85984-ref12">12</xref>]</td><td align="center" valign="middle" >Ammonium ( NH 4 + )</td></tr><tr><td align="center" valign="middle" >Titrimetrically using:</td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >(a) Silver nitrate and potassium dichromate as indicator [<xref ref-type="bibr" rid="scirp.85984-ref14">14</xref>]</td><td align="center" valign="middle" >Chloride (Cl)</td></tr><tr><td align="center" valign="middle" >(b) Complexometric technique using EDTA as Titrant [<xref ref-type="bibr" rid="scirp.85984-ref12">12</xref>] .</td><td align="center" valign="middle" >Calcium (Ca), Magnesium (mg)</td></tr><tr><td align="center" valign="middle" >(c) Titration using indicators like muraxide etc.</td><td align="center" valign="middle" >Total hardness, bizabonate and alkalinity</td></tr><tr><td align="center" valign="middle" >Difference between initial oxygen concentration in sample and concentration after 5 days incubation in DO bottles at 20˚C [<xref ref-type="bibr" rid="scirp.85984-ref12">12</xref>]</td><td align="center" valign="middle" >Biochemical Oxygen demand, BOD5</td></tr></tbody></table></table-wrap><p>H1: Soil chemical properties significantly vary in the study locations.</p><p>Estimates in variation of soil physical characteristics (sand, silt, and clay contents) were analysed using analysis of variance (ANOVA) for the four different sites across the study locations. Thus, ANOVA was adopted to see if variation occurs in sand, silt and clay contents across sites.</p></sec><sec id="s5"><title>5. Results and Discussion of Findings</title><sec id="s5_1"><title>5.1. Physical Characteristics of the Soil</title><p>The physical properties of the soils sampled from the prescribed study area are summarized in <xref ref-type="table" rid="table2">Table 2</xref>. The sand, silt and clay fractions varied in texture along the sampling locations. The texture of the soils sampled along the stations varied from sandy loam and loamy sand texture depending on the pedogenesis and edaphic features of the study area. Soil texture determines water intake, storage capacity, ease of tillage and amount of aeration influencing its fertility capability and status [<xref ref-type="bibr" rid="scirp.85984-ref15">15</xref>].</p><p>Sand fractions ranged from 67.28% to 77.01% with a mean value of 82.80% (surface soils) and between 64.92% to 76.11% with a mean of 68.74% (subsurface soils); Silt from 12.90% to 28.46% and 13.20% to 29.24% with means of 20.23% and 21.78% respectively; clay contents from 1.90% to 14.90% and 2.54% to 24.62% with means of 7.19% and 9.48% respectively for surface and subsurface soil samples collected from the study area (<xref ref-type="table" rid="table2">Table 2</xref>). This high sand content of the soils is characterized by sand formed on unconsolidated coastal plain sand and sandstones. Since sandy soil is not fit for crop production, the presence of oil-spill which significantly increased the percentage sand has adverse effect on the fertility of the affected soils. This is as a result of a probable high drainage of oil into the lower horizon of the soil causing aeration problem as the air pores get blocked with oil, which prevent the easy flow of nutrients to the soil (<xref ref-type="fig" rid="fig2">Figure 2</xref>). The soils are characterized as coarse-textured with a high proportion of sand fraction exceeding 70%. Such soils lack adsorptive capacity for basic plant nutrients and water. Consequently, such soils have weak surface aggregation and are vulnerable to erosion [<xref ref-type="bibr" rid="scirp.85984-ref15">15</xref>].</p></sec><sec id="s5_2"><title>5.2. Chemical Characteristics of the Soil</title><p>The chemical properties of the soils under study are summarized in <xref ref-type="table" rid="table3">Table 3</xref> in relation to the sampling stations. Basically, results obtained from laboratory analyses were compared with acceptable conditions under which crops can thrive in the study area [<xref ref-type="bibr" rid="scirp.85984-ref8">8</xref>]. It is discussed under the following nutrient concentrations:</p><sec id="s5_2_1"><title>5.2.1. Soil pH</title><p>Soil pH is fundamental to the understanding of soil systems, because it is an indicator of many reactions in the soils. It shows whether the soil is acidic, neutral or basic and provides useful information on the availability of the exchangeable</p><p>cations. Soil pH controls plant nutrient availability and microbial reactions in soils. The pH of the air-dried soils ranged from 4.2 to 5.8 (surface soils) and 4.2 to 5.6 (subsurface soils) with means of 5.0 and 5.3 respectively (<xref ref-type="table" rid="table3">Table 3</xref>) within Khana, Gokana, Eleme and Tai study locations. This depicts strong acidity in the ecological zone. The project environment is strongly acidic across the sampling stations due to the leaching of basic cations from the soil solum. Such acidic soil condition can induce phosphate fixation and consequently reduce the ability of microbes to fix atmospheric nitrogen. The strong acid condition indicates that certain elements such as Zinc, Iron, Manganese and Aluminium are available in soils of the study environment. The soils are all slightly acidic and this acidity cannot be attributed entirely to the oil spill since other non-oil producing areas such as Yeghe and Kpong are equally acidic. The acidity is typical of the soils of southern part of Nigeria and is ascribed to the excessive precipitation which leads to leaching loose of most of the basic cations in the soil [<xref ref-type="bibr" rid="scirp.85984-ref16">16</xref>].</p></sec><sec id="s5_2_2"><title>5.2.2. Electrical Conductivity (EC)</title><p>The electrical conductivity values varied from 0.025 to 0.049 dSm<sup>−</sup><sup>1</sup> (surface soils) while 0.026 to 0.500 dSm<sup>−</sup><sup>1</sup> were recorded for the subsurface soil samples in all the sampling stations (<xref ref-type="table" rid="table3">Table 3</xref>). This range of values indicates that the soils are non-saline as all the values along the stations are below 4 dSm<sup>−</sup><sup>1</sup> [<xref ref-type="bibr" rid="scirp.85984-ref17">17</xref>] and do not exceed the critical value of 2 dSm<sup>−</sup><sup>1</sup> for sensitive crop species [<xref ref-type="bibr" rid="scirp.85984-ref18">18</xref>]. These results suggest that the soils do not have salinity problem.</p></sec><sec id="s5_2_3"><title>5.2.3. Organic Carbon and Total Nitrogen contents</title><p>Carbon is an essential plant nutrient and the foundation of all life [<xref ref-type="bibr" rid="scirp.85984-ref19">19</xref>]. Carbon compound are enzymatically oxidized to produce carbon dioxide, water, energy, and decomposer biomass. Soil organic matter contributes to soil aggregation and</p><p>reduces susceptibility to erosion [<xref ref-type="bibr" rid="scirp.85984-ref20">20</xref>]. The organic carbon in soils of the study area ranged from 1.00% to 3.80% and between 1.00% to 2.79% for surface and subsurface soils respectively across the prescribed stations (<xref ref-type="table" rid="table3">Table 3</xref>). Such soils are rated medium in fertility status [<xref ref-type="bibr" rid="scirp.85984-ref21">21</xref>]. The results indicate that organic carbon decreases with depth in all the stations used for this study. From the results, Tai station recorded the highest level of organic carbon contents, while the lowest content was obtained in Eleme, Tai and Khana of the ecological zone. Thus, in spite of the level of pollution, the soils can sustain crop production in the ecological zone.</p><p>Nitrogen in the form of protein is present in the protoplasm of every cell. The available form of Nitrogen in the soil is ammonium or nitrate ion. Total nitrogen varied from 0.03% to 0.08% and 0.02% to 0.06% for surface and subsurface soils respectively in all the stations sampled for this study (<xref ref-type="table" rid="table3">Table 3</xref>). This range of values is rated low when compared with the medium range of 0.10% to 0.45% [<xref ref-type="bibr" rid="scirp.85984-ref21">21</xref>] for soils of the area under study. In a similar manner, total nitrogen decreases with depth in all the stations where the samples were collected. These locational ranges of values is consistent with the works of [<xref ref-type="bibr" rid="scirp.85984-ref22">22</xref>] who reported average total percentage of 0.08 in soils of the Cross River Coastal plain sands and mean range of 0.10% to 0.14% reported by Abii and Nwosu [<xref ref-type="bibr" rid="scirp.85984-ref4">4</xref>] for surface and subsurface soils of Eleme in Rivers State. Thus, there is variation in the contents of total nitrogen in this ecological zone.</p></sec><sec id="s5_2_4"><title>5.2.4. Available Phosphorus and Exchangeable Cations</title><p>Phosphorus is an essential part of nucleoprotein in the cells nuclei, which control cell division and growth, and of deoxyribonucleic acid (DNA) molecules. In the study environment, available phosphorous ranged from 6 to 12 mg kg<sup>−1</sup> (surface soils) and between 7 to 14 mg kg<sup>−1</sup> for the subsurface soil samples (<xref ref-type="table" rid="table3">Table 3</xref>). Available P contents were generally moderate (i.e. polluted, but not significantly) in all the stations as values are below 15 mg kg<sup>−1</sup> [<xref ref-type="bibr" rid="scirp.85984-ref15">15</xref>]. This range of values is consistent with the findings of Ekundayo [<xref ref-type="bibr" rid="scirp.85984-ref16">16</xref>] who reported near mean value of 10 mg kg<sup>−1</sup> for arable soils of South-Eastern Nigeria.</p><p>The exchangeable cations (Ca, Mg, K and Na) are positively charged ions usually absorbed by electrostatic or columbic attraction to soil surface colloids. Plants absorb it in exchangeable form [<xref ref-type="bibr" rid="scirp.85984-ref21">21</xref>]. The exchangeable cations for the surface soils were as follows: Ca (0.56 - 3.22 cmol kg<sup>−1</sup>); Mg (1.09 - 6.24 cmol kg<sup>−1</sup>); K (1.00 - 3.11 cmol kg<sup>−1</sup>) and Na (0.01 - 0.26 cmol kg<sup>−1</sup>). Conversely, the following values were recorded for the subsurface soils: Ca (0.40 to 3.01 cmol kg<sup>−1</sup>); Mg (1.00 - 4.11 cmol kg<sup>−1</sup>); K (0.08 to 3.11 cmol kg<sup>−1</sup>) (&lt;10.0 cmol kg<sup>−1</sup>) for both surface and subsurface soils. Magnesium was moderate for both seasons and Na (0.05 to 0.28 cmol kg<sup>−1</sup>) (<xref ref-type="fig" rid="fig3">Figure 3</xref>); K was considerable (&gt;1.2 cmol kg<sup>−1</sup>) in both surface and subsurface soils and Na was within the permissible limits (&gt;0.3 cmol kg<sup>−1</sup>) (<xref ref-type="table" rid="table3">Table 3</xref>). Thus, there is a slight locational difference among the exchangeable bases in soils of the environment. In sum, there is a low cation reserve in the soils.</p></sec><sec id="s5_2_5"><title>5.2.5. Effective Cation Exchange Capacity (ECEC)</title><p>Effective cation exchange capacity (ECEC) was very moderate in both surface and subsurface soils with range values of 5.87 to 11.67 cmol kg<sup>−1</sup> recorded in the surface soils compared to range values of 4.77 to 10.92 cmol kg<sup>−1</sup> for the subsurface soil samples (&gt;10.0 cmol kg<sup>−1</sup>).</p></sec><sec id="s5_2_6"><title>5.2.6. Exchange Acidity (EA)</title><p>Exchange acidity value for the surface soils (1.22 in Tai LGA to 3.81 cmol kg<sup>−1</sup> in Khana LGA) and subsurface soils (1.24 to 8.06 cmol kg<sup>−1</sup>) were above range when compared with a medium range of 2.1 to 4.1 cmol kg<sup>−1</sup> (<xref ref-type="table" rid="table2">Table 2</xref>), Albeit impact of Al<sup>3+</sup> in the soil solution could be significant in terms of influencing the biochemical behaviour of these soils.</p></sec><sec id="s5_2_7"><title>5.2.7. Percent Base Saturation (PBS)</title><p>Percent base saturation ranged from 60 to 90 and 55 to 85 for surface and subsurface soil samples respectively across the prescribed stations under study (<xref ref-type="table" rid="table3">Table 3</xref>). With the mean percentage base saturation above the threshold limits in surface and subsurface soil samples, basic nutrient must have occurred in available forms in soil solution in spite of the low cation reserves across the stations. This collaborate earlier results of Abii et al. [<xref ref-type="bibr" rid="scirp.85984-ref4">4</xref>] on the effect of oil spillage on the soil of Eleme, Rivers State, which indicated that base saturation range of 60 - 85 and 50 - 80 for surface and subsurface respectively.</p></sec></sec><sec id="s5_3"><title>5.3. Heavy Metal Status</title><p>The heavy metals status in soils of the study area is summarized in <xref ref-type="table" rid="table4">Table 4</xref>. Heavy metals exist in variable oxidation states, particularly those that belong to the d-group of the periodic table, each with different reactive, toxicological,</p><p>physiological and bioconcentration potential. Though some heavy metals, such as Cadium (Cd), Lead (Pb) and Zinc (Zn) are toxic in their cationic form, many others require biochemical transformation to organic metallic compounds [<xref ref-type="bibr" rid="scirp.85984-ref23">23</xref>].</p><sec id="s5_3_1"><title>5.3.1. Iron (Fe) and Manganese (Mn) Status</title><p>Iron is one of the most abundant elements in the earth’s crust with variable oxidation states of +2 and +3 (Fe<sup>2+</sup> and Fe<sup>3+</sup>). In the study environment, mean iron contents ranged from 426.11 to 6701.04 mg kg<sup>−1</sup> (surface soils) and between 262.06 to 6626.11 mg kg<sup>−1</sup> for the subsurface soils (<xref ref-type="table" rid="table4">Table 4</xref>). The results show that iron contents were high in surface soils compared to the subsurface soils. This range of values is within the natural limits for mineral soil environment [<xref ref-type="bibr" rid="scirp.85984-ref20">20</xref>].</p><p>Manganese contents ranged from 111.20 to 313.20 mg kg<sup>−1</sup> (surface soils) and between 109.00 to 313.20 mg kg<sup>−1</sup> in the sub-surface soils (<xref ref-type="table" rid="table4">Table 4</xref>). There are mild variations in the mean contents of this parameter. In spite of these, manganese contents are within the tolerable limits established for mineral soil environment [<xref ref-type="bibr" rid="scirp.85984-ref20">20</xref>].</p></sec><sec id="s5_3_2"><title>5.3.2. Copper (Cu) and Zinc (Zn) Status</title><p>Copper (Cu) is an essential micronutrient required for plant growth, and is normally found in soils only in trace amount. Copper can be retained in soils by adsorption via non-specific and specific interactions, as well as precipitation reaction with hydroxides, carbonates, phosphates and silicates [<xref ref-type="bibr" rid="scirp.85984-ref24">24</xref>]. In the study area, Copper contents varied from 0.19 to 1.44 mg kg<sup>−1</sup> (surface soils) while the range value of 0.40 to 6.26 mg kg<sup>−1</sup> were recorded for the subsurface soils (<xref ref-type="table" rid="table4">Table 4</xref>). Comparatively, mean copper contents were high in the surface soils compared to the subsurface soils of the study area. This range of values is within the maximum permissible limits of 2 - 100 mg kg<sup>−1</sup> [<xref ref-type="bibr" rid="scirp.85984-ref25">25</xref>] established for mineral soil environment. With the low contents of Cu therefore, the study area is safe in terms of water quality, crop growth and ecological sustainability [<xref ref-type="bibr" rid="scirp.85984-ref24">24</xref>].</p><p>Zinc is mainly found in rock forming minerals. It could be present in industrial discharges and not considered very toxic to humans or other organism. The mean concentration of zinc in soils within the Ogoni kingdom varied from 2.16 to 56.26 mg kg<sup>−1</sup> for the surface soils and 0.19 - 6.26 mg kg<sup>−1</sup> for the subsurface soil samples (<xref ref-type="table" rid="table4">Table 4</xref>). Though, this dose of concentration is within the threshold limits of 10 - 300 mg kg<sup>−1</sup> established for mineral soils [<xref ref-type="bibr" rid="scirp.85984-ref25">25</xref>].</p></sec><sec id="s5_3_3"><title>5.3.3. Chromium (Cr) and Nickel (Ni)</title><p>Concentration of chromium varied from 1.00 to 6.26 mg kg<sup>−1</sup> (surface soils) and between 1.00 to 3.68 mg kg<sup>−1</sup> (subsurface soils) (<xref ref-type="table" rid="table4">Table 4</xref>). Comparatively, Chromium contents were slightly higher in the surface soils in contrast to the subsurface soils in the area (<xref ref-type="table" rid="table4">Table 4</xref>). Despite the concentration, it is still within the safe limits of 5 - 1000 mg kg<sup>−1</sup> established for mineral soil environments [<xref ref-type="bibr" rid="scirp.85984-ref25">25</xref>] [<xref ref-type="bibr" rid="scirp.85984-ref26">26</xref>].</p><p>Nickel concentration in soils of the study area varied from 11.28 to 28.74 mg kg<sup>−1</sup> and 10.00 - 26.09 mg kg<sup>−1</sup> respectively for surface and subsurface soils (<xref ref-type="table" rid="table4">Table 4</xref>). Comparatively, Nickel was slightly higher in the subsurface soils in contrast to the subsurface soils. This range of values is within the allowable limits of 1 - 1000 mg kg<sup>−1</sup> established for mineral soil environment [<xref ref-type="bibr" rid="scirp.85984-ref25">25</xref>] [<xref ref-type="bibr" rid="scirp.85984-ref26">26</xref>].</p></sec><sec id="s5_3_4"><title>5.3.4. Lead (pb) and Cadmium (Cd)</title><p>Mean lead (Pb) contents are low and ranged from 1.26 to 28.10 mg kg<sup>−1</sup> and 1.80 to 20.12 mg kg<sup>−1</sup> for surface soils and subsurface soils respectively (<xref ref-type="table" rid="table4">Table 4</xref>). Thus, mild variation in Pb contents in soils of the study area was recorded. These values are within the maximum tolerable limits of 2 - 200 mg kg<sup>−1</sup> for the soils ecological settings [<xref ref-type="bibr" rid="scirp.85984-ref25">25</xref>] [<xref ref-type="bibr" rid="scirp.85984-ref26">26</xref>].</p><p>Cadmium is a non-essential element that diminishes growth of organisms. It is widely spread but occurs in mild concentration and considered a potential carcinogen. The concentration of Cadmium varied from 1.02 to 3.28 mg kg<sup>−1</sup> and between 0.80 to 26.09 mg kg<sup>−1</sup> for surface soils and subsurface soils respectively (<xref ref-type="table" rid="table4">Table 4</xref>), though the concentration of Cadmium is within the tolerable limits established by FEPA [<xref ref-type="bibr" rid="scirp.85984-ref27">27</xref>].</p></sec><sec id="s5_3_5"><title>5.3.5. Vanadium (V)</title><p>Vanadium contents varied from 1.41 to 8.28 mg kg<sup>−1</sup> and 1.06 to 8.96 mg kg<sup>−1</sup> for surface and subsurface soils respectively (<xref ref-type="table" rid="table4">Table 4</xref>). This range is within the threshold limits of 20 - 500 mg kg<sup>−1</sup> for soils of the ecological zone under consideration [<xref ref-type="bibr" rid="scirp.85984-ref25">25</xref>].</p></sec><sec id="s5_3_6"><title>5.3.6. Total Hydrocarbon Content (THC)</title><p>Petroleum is a naturally occurring complex mixture of organic compounds formed from decomposition of fossil materials overtime. Total hydrocarbon contents (THC) of the soils was very high as evident in the mean of 259.92 and 192.60 mg kg<sup>−1</sup> in surface and subsurface soils respectively of the project area (<xref ref-type="table" rid="table4">Table 4</xref>). The high THC values above 100 mg kg<sup>−1</sup> in the soils indicate that the soils are polluted by petroleum source contaminants [<xref ref-type="bibr" rid="scirp.85984-ref28">28</xref>].</p><p>In summary, despite the values of the heavy metals, the soils are within the threshold limits established in typical mineral soils [<xref ref-type="bibr" rid="scirp.85984-ref25">25</xref>] [<xref ref-type="bibr" rid="scirp.85984-ref20">20</xref>]. The high contents of nickel and vanadium (often associated with heavy metals) may be attributed to the activities in the region although the nature of the parent materials may not give sufficient explanation. Thus, the soils of the study area are declared contaminated by heavy metals and hydrocarbon toxicity. Taking cognizance of the sandy loam and loamy sand texture, medium organic carbon contents, the soils have weak surface aggregation and inherently low fertility status. <xref ref-type="fig" rid="fig4">Figure 4</xref> further attempts a coefficient of determination, R<sup>2</sup> which is a measure of the total variation in surface soils in Ogoni region that is explained statistically or determined by the distribution of subsurface soil. Therefore, a coefficient of determination, R<sup>2</sup>, of 0.005 and 0.001 for both surface and subsurface respectively indicates that none of the variation in surface soil can be attributed to a linear</p><p>relation with the subsurface soil. The results also show a none-linear relationship in different soil texture, though with high variation between sample points.</p></sec></sec><sec id="s5_4"><title>5.4. Test of Research Hypothesis 1</title><sec id="s5_4_1"><title>5.4.1. Hypothesis 1</title><p>H<sub>0</sub>: Soil physical properties do not significantly vary in the study locations.</p><p>H<sub>1</sub>: Soil physical properties significantly vary in the study locations.</p><p>Estimates in variation of soil physical characteristics (sand, silt, and clay contents) are succinctly presented in <xref ref-type="table" rid="table5">Table 5</xref> using analysis of variance (ANOVA) for the four different sites across the study locations. Thus, ANOVA was adopted to see if variation occurs in sand, silt and clay contents across sites. From the result, sand, silt and clay contents significantly vary in Eleme, Tai, Khana and Gokhana as evident in the calculated F-ratios of 307.6958; 606.7196; 1312.3140; and 1154.0230 respectively against the tabulated F-ratio of 3.3541 at reflected by the wide variations in sand, silt and clay fractions, probably owing to differences in parent material, influence of the 1% level of significance (see <xref ref-type="table" rid="table5">Table 5</xref>). Therefore, the alternate hypothesis that soil physico-chemical properties significantly vary in the study locations is accepted. The results posit that soil physical characteristics changes with site as (1998) in the Niger Delta significantly show wide variations based on the reasons outlined earlier. Soil physical parameters are relevant in understanding the genesis, their lithology and its morphological features.</p><table-wrap id="table2" ><label><xref ref-type="table" rid="table5">Table 5</xref></label><caption><title> ANOVA summary results showing variations in soil physical characteristic</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Source of variation</th><th align="center" valign="middle" >Sum of squares</th><th align="center" valign="middle" >Df</th><th align="center" valign="middle" >Mean square</th><th align="center" valign="middle" >F-ratio</th><th align="center" valign="middle" >D-value</th></tr></thead><tr><td align="center" valign="middle" >Between Groups Within Groups Total Between Groups Within Groups Total Between Groups Within Groups Total Between Groups Within Groups Total</td><td align="center" valign="middle" >21,226.15 931.2867 22,157.44 20,211.97 449.7327 20,661.71 23,232 238.9916 23,470.99</td><td align="center" valign="middle" >Eleme site 2 27 29 Tai site 2 27 29 Khana site 2 27 29 Gokana site 2 27 29</td><td align="center" valign="middle" >10,613.08 34.4921 10,105.99 16.6568 11,616 8.8515 12,448.13 10.7867</td><td align="center" valign="middle" >307.6958*** 606.7196*** 1312.3140*** 1154.0230***</td><td align="center" valign="middle" >2.16886E−19 3.63197E−23 1.27641E−27 7.10267E−27</td></tr></tbody></table></table-wrap><p>Note: F-critical = 3.3541; *** = significant at 1% level.</p></sec><sec id="s5_4_2"><title>5.4.2. Hypothesis 2</title><p>H<sub>0</sub>: Soil chemical properties do not significantly vary in the study locations.</p><p>H<sub>1</sub>: Soil chemical properties significantly vary in the study locations.</p><p>The summary result of the soils around the Ogoni area is shown in <xref ref-type="table" rid="table6">Table 6</xref> based on the locations where the soils were sampled. Essentially, the analysis of variance (ANOVA) was used to test the extent of variance in the chemical properties. The choice of ANOVA was informed by the nature of the soils around the operating oil companies. The results presented indicates that the soils vary widely in chemical characteristics in Eleme (F-ratio = 1674.1630; p &lt; 0.01; F-critical = 1.8784); Tai (F-ratio = 82.6671; p &lt; 0.01; F-critical = 1.8784); Khana (F-ratio = 1467.6620; p &lt; 0.01; F-critical = 1.8784) and Gokhana (F-ratio = 709.4864; p &lt; 0.01; F-critical = 1.8784) against the theoretical value as shown in <xref ref-type="table" rid="table6">Table 6</xref>. Therefore in testing this hypothesis, the alternate that chemical property of soil vary across different study locations in the study area. Clearly, the variation in the chemical properties in all the sites sampled may be ascribed to variation in the soil parent material, the stage of pedogenetic formation where the soils were formed. Thus, an understanding of the chemical characteristics is guided mostly by the factors outlined above.</p></sec></sec><sec id="s5_5"><title>5.5. Testing of Research Hypothesis</title><p>The results posit that soil physical characteristics which are relevant in understanding the genesis, their lithology and their morphological features changes with site as at (2010) in the Niger Delta significantly show wide variations based</p><table-wrap id="table3" ><label><xref ref-type="table" rid="table6">Table 6</xref></label><caption><title> ANOVA summary result showing variations in soil chemical characteristics</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Source of variation</th><th align="center" valign="middle" >Sum of squares</th><th align="center" valign="middle" >Df</th><th align="center" valign="middle" >Mean square</th><th align="center" valign="middle" >F-ratio</th><th align="center" valign="middle" >D-value</th></tr></thead><tr><td align="center" valign="middle" >Between Groups Within Groups Total Between Groups Within Groups Total Between Groups Within Groups Total Between Groups Within Groups Total</td><td align="center" valign="middle" >39,001.50 228.7255 39,230.22 41,360.01 4866.7490 46,226.76 44,713.15 299.1246 45,012.27</td><td align="center" valign="middle" >Eleme site 11 108 119 Tai site 11 108 119 Khana site 11 108 119 Gokana site 11 108 119</td><td align="center" valign="middle" >3545.5910 2.117829 3760.0010 45.48363 4064.8320 2.7696 3996.32 5.632694</td><td align="center" valign="middle" >1674.1630*** 82.6671*** 1467.6220*** 709.4864***</td><td align="center" valign="middle" >3.2E−115 4.35016E−47 3.7745E−112 2.77665E−95</td></tr></tbody></table></table-wrap><p>Note: F-critical = 1.8784; *** = significant at 1% level.</p><p>on wide variations in sand, silt and clay fractions, and probably owing to differences in parent material. Clearly, the variation in the chemical properties in all the sites sampled may be ascribed to variation in the soil parent material and the stage of pedogenetic formation. Thus, an understanding of the chemical characteristics is guided mostly by the factors outlined above.</p></sec></sec><sec id="s6"><title>6. Conclusions</title><p>The physical properties of the soils sampled in terms of sand, silt and clay fractions varied in texture along the sampling locations. The texture of the soils sampled along the stations varied from sandy loam and loamy sand texture depending on the pedogenesis and edaphic features of the study area. For example, Tai and Khana LGAs correspond to areas with loam sandy and sandy loam for surface and subsurface soils respectively, while Gokana and Eleme LGAs indicates sandy loam and loamy sand respectively for surface and subsurface soils. The soils are characterized as coarse-textured with a high proportion of sand fraction exceeding 70%, 22% for silt and 8% for clay fractions. Thus, the soils of the prescribed study area are declared contaminated by heavy metals and hydrocarbon toxicity, though contaminated by metals but not significant. Also taking cognizance of the sandy loam and loamy sand texture, medium in organic carbon contents, the soils have weak surface aggregation and inherently medium in fertility status.</p><p>From the result, sand, silt and clay contents significantly vary in Eleme, Tai, Khana and Gokhana as evident in the calculated F-ratios of 307.70; 606.72; 1312.31; and 1154.02 respectively against the tabulated F-ratio of 3.35 as reflected by the wide variations in sand, silt and clay fractions, and probably owing to differences in parent material. Conversely, the summary result of the soils around the Ogoni area based on the locations where the soils were sampled. The results presented indicate that the soils vary widely in chemical characteristics in Eleme (F-ratio = 1674.16; p &lt; 0.01; F-critical = 1.88); Tai (F-ratio = 82.67; p &lt; 0.01; F-critical = 1.88); Khana (F-ratio = 1467.66; p &lt; 0.01; F-critical = 1.88) and Gokhana (F-ratio = 709.49; p &lt; 0.01; F-critical = 1.88) against the theoretical value. Therefore, the alternate hypothesis that soil physico-chemical properties significantly vary in the study locations is accepted.</p></sec><sec id="s7"><title>7. Recommendations</title><p>The oil industry has undoubtedly brought economic benefits to many people, but it has left its trail a complex mix of environmental pollution problems, the most notable of which is oil pollution and physical destruction of landscape. Preventing environmental degradation is a task that must be vigorously pursued judging from the enormous economic loss and environmental destruction arising from oil exploration, transportation and marketing. From the findings in this study, the following recommendations are therefore made:</p><p>1) There is need to ensure effective enforcement of National Environmental Standards Agency (NESA) regulation, guidelines and standards, which arises from researches and critical observation of environmental situation in the oil producing areas.</p><p>2) In order to protect and preserve our environment from pollution caused by petroleum related operations, a long term and comprehensive environmental monitoring programme should be instituted. The monitoring programme however should have as its basis the provision for establishment of comprehensive environmental base data in Nigeria.</p><p>3) There is need for the inclusion of communities in the echelon of oil spill management. The role of the communities committee, which will be made up of all stakeholders of the producing areas can be affected through a community oil spill committee. The General operational guidelines of the committee may be provided by the government through the Nigerian National Petroleum Corporation.</p></sec><sec id="s8"><title>Cite this paper</title><p>Okon, I.E. and Ogba, C.O. (2018) The Impacts of Crude Oil Exploitation on Soil in Some Parts of Ogoni Region, Rivers State, Southern Nigeria. Open Access Library Journal, 5: e4297. https://doi.org/10.4236/oalib.1104297</p></sec></body><back><ref-list><title>References</title><ref id="scirp.85984-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">Andrew, R. (1994) Shell Shocked: The Environmental and Social Cost of Living with Shell in Nigeria. Greenpeace International, Amsterdam, 32-47.</mixed-citation></ref><ref id="scirp.85984-ref2"><label>2</label><mixed-citation publication-type="other" xlink:type="simple">Pyagbara, L.S. (2007) The Adverse Impacts of Oil Pollution on the Environment and Wellbeing of a Local Indigenous Community: The Experience of the Ogoni. Department of Economic and Social Affairs, New York.</mixed-citation></ref><ref id="scirp.85984-ref3"><label>3</label><mixed-citation publication-type="other" xlink:type="simple">Azaiki, S. (2003) Inequalities in Nigerian Politics: The Niger Delta, Resource Control, Underdevelopment and Youth Restiveness. Treasure Books, Yenagoa, 55-73.</mixed-citation></ref><ref id="scirp.85984-ref4"><label>4</label><mixed-citation publication-type="other" xlink:type="simple">Abii, T.A. and Nwosu, P.C. (2009) The Effect of Oil-Spillage on the Soil of Eleme in Rivers State of the Niger-Delta Area of Nigeria. Research Journal of Environmental Sciences, 3, 316-320. https://doi.org/10.3923/rjes.2009.316.320</mixed-citation></ref><ref id="scirp.85984-ref5"><label>5</label><mixed-citation publication-type="other" xlink:type="simple">Amanyie, V. (2001) The Agony of the Ogony in the Niger Delta: A Case Study. Horizon Concepts, 19-37.</mixed-citation></ref><ref id="scirp.85984-ref6"><label>6</label><mixed-citation publication-type="other" xlink:type="simple">National Population Commission (NPC) (2006) Nigerian Population Census Report. National Population Commission, Abuja, 21-27.</mixed-citation></ref><ref id="scirp.85984-ref7"><label>7</label><mixed-citation publication-type="book" xlink:type="simple">Gee, G.W. and Bauder, J.W. (1986) Particle Size Analysis. In: Klute, A., Ed., Methods of Soils Analysis, American Society of Agronomy, Madison, Vol. 2, 47-56.</mixed-citation></ref><ref id="scirp.85984-ref8"><label>8</label><mixed-citation publication-type="other" xlink:type="simple">International Institute for Tropical Agriculture (IITA) (1979) Selected Methods for Soil and Plant Analysis. Manual Series 1, Ibadan, 1-70.</mixed-citation></ref><ref id="scirp.85984-ref9"><label>9</label><mixed-citation publication-type="other" xlink:type="simple">Walkley, A. and Black, I.A. (1934) An Examination of the Degtijarett Method of Determining Soil Matter and Proposed Modification of the Chromic Acid Titration Method. Soil Science Society of American Journal, 37, 29-38. https://doi.org/10.1097/00010694-193401000-00003</mixed-citation></ref><ref id="scirp.85984-ref10"><label>10</label><mixed-citation publication-type="other" xlink:type="simple">Bray, R.H. and Kurtz, L.T. (1945) Determination of Total, Organic and Available Forms of Phosphorous in Soils. Soil Science, 5, 39-45. https://doi.org/10.1097/00010694-194501000-00006</mixed-citation></ref><ref id="scirp.85984-ref11"><label>11</label><mixed-citation publication-type="other" xlink:type="simple">Barnhisel, R. and Bertsch, P.M. (1982) Aluminum in Methods of Soil Analysis, Part 2. Agronomy, 9, 275-300.</mixed-citation></ref><ref id="scirp.85984-ref12"><label>12</label><mixed-citation publication-type="other" xlink:type="simple">APHA (1989) Standard Methods for the Examination of Water and Wastewater. 17th Edition, APHA-AWWA-WPCF, Washington DC.</mixed-citation></ref><ref id="scirp.85984-ref13"><label>13</label><mixed-citation publication-type="other" xlink:type="simple">Parsons, F., Wood, P.R. and DeMarco, J. (1984) Transformations of Tetrachloroethene and Trichloroe-thene in Microcosms and Groundwater. Journal (American Water Works Association), 76, 56-59. https://doi.org/10.1002/j.1551-8833.1984.tb05282.x</mixed-citation></ref><ref id="scirp.85984-ref14"><label>14</label><mixed-citation publication-type="other" xlink:type="simple">Rodier, J. (1975) An Analysis of Water, Natural Water, Waste Water, Sea Water: Chemistry, Bacteriology, Biology. Dunod.</mixed-citation></ref><ref id="scirp.85984-ref15"><label>15</label><mixed-citation publication-type="other" xlink:type="simple">Fertilizer Procurement and Distribution Division (FPDD) (1990) Literature Review on Soil Fertility Investigations in Nigeria (in Five Volumes) Produced by the Federal Ministry of Agriculture and Natural Resources, Lagos. Federal Ministry of Ag-riculture and Natural Resources (FMANR), 6, 133-159.</mixed-citation></ref><ref id="scirp.85984-ref16"><label>16</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>Ekundayo</surname><given-names> E.O. </given-names></name>,<etal>et al</etal>. (<year>2004</year>)<article-title>In-fluence of Different Soil Types on Abundance of Vesicular-Arbuscal or Mycorrhizal Fungi in Arable Soils of Southern Nigeria</article-title><source> Nigeria Journal of Soil Science</source><volume> 14</volume>,<fpage> 40</fpage>-<lpage>47</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.85984-ref17"><label>17</label><mixed-citation publication-type="other" xlink:type="simple">Miller, R.W. and Donahue, R.L. (1995) Soils in Our Environment. Prentice-Hall, London.</mixed-citation></ref><ref id="scirp.85984-ref18"><label>18</label><mixed-citation publication-type="other" xlink:type="simple">Food and Agriculture Organization (FAO) (1974) Soil Map of the World. Volume 1, Legend, Paris.</mixed-citation></ref><ref id="scirp.85984-ref19"><label>19</label><mixed-citation publication-type="other" xlink:type="simple">Magdoff, F. (1992) Building Soils for Better Crops. Nebraska Press, Lincoln.</mixed-citation></ref><ref id="scirp.85984-ref20"><label>20</label><mixed-citation publication-type="other" xlink:type="simple">Brady, N.C. and Weil, R.R. (1996) The Nature and Properties of Soils. Eleventh Edition, Prentice-Hall, Upper Saddle River, New York.</mixed-citation></ref><ref id="scirp.85984-ref21"><label>21</label><mixed-citation publication-type="other" xlink:type="simple">Holland, M.D., Allen, R.K.G., Barten, D. and Murphy, S.T. (1989) Land Evaluation and Agricultural Recommendations for Cross River National Park, Oban Division. Prepared by the Overseas Development Resources Institute in Collaboration with WWF for the Federal Republic of Nigeria and the Cross River State Government, 7, 34-76.</mixed-citation></ref><ref id="scirp.85984-ref22"><label>22</label><mixed-citation publication-type="other" xlink:type="simple">Ukaegbu, E.P. and Akamigbo, F.O.R. (2005) Influence of Physiography on the Properties and Use of Soils of the Cross River Plain: A Case Study of a Strip of Land at Isiagu, Ebonyi State. Soil Science Society, 103-109.</mixed-citation></ref><ref id="scirp.85984-ref23"><label>23</label><mixed-citation publication-type="other" xlink:type="simple">Donahue, R.L., Miller, R.W. and Shickluna, T.C. (1990) An Introduction to Soils and Plant Growth. Prentice-Hall of India, New Delhi.</mixed-citation></ref><ref id="scirp.85984-ref24"><label>24</label><mixed-citation publication-type="other" xlink:type="simple">McLaren, R.G. (2003) Micronutrients and Toxic Elements. Handbook of Processes and Modeling in the Soil-Plant System. Haworth Press, New York.</mixed-citation></ref><ref id="scirp.85984-ref25"><label>25</label><mixed-citation publication-type="other" xlink:type="simple">Bohn, H.L., McNeal, B.L. and O’Connor, G.A. (1995) Soil Chemistry. John Wiley and Sons, New York.</mixed-citation></ref><ref id="scirp.85984-ref26"><label>26</label><mixed-citation publication-type="book" xlink:type="simple">Mortvedt, J.J. (2000) Bioavailability of Micronutrients. In: Summer, M.E., Ed., Handbook of Soil Science, CRC Press, Boca Raton.</mixed-citation></ref><ref id="scirp.85984-ref27"><label>27</label><mixed-citation publication-type="other" xlink:type="simple">Federal Environmental Protection Agency (FEPA) (1988) Guideline and Standards for Environmental Pollution Control in Nigeria. Federal Government, Lagos, 238-291.</mixed-citation></ref><ref id="scirp.85984-ref28"><label>28</label><mixed-citation publication-type="other" xlink:type="simple">Line, M.A., Gerland, C.O. and Crowley, M. (1997) Evaluation of Landform Remediation of Hydrocarbon-Contaminated Soils at the Inveresk Railyard, Launceston, Australia. Waste Management, Elsevier Science, 16, 567-569. https://doi.org/10.1016/S0956-053X(96)00077-3</mixed-citation></ref></ref-list></back></article>