<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">CM</journal-id><journal-title-group><journal-title>Chinese Medicine</journal-title></journal-title-group><issn pub-type="epub">2151-1918</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/cm.2018.91001</article-id><article-id pub-id-type="publisher-id">CM-82826</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine&amp;Healthcare</subject></subj-group></article-categories><title-group><article-title>
 
 
  Effect of Blue Colour (453 nm Visible Range Radiation) on Anxiety in College Students
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Samina</surname><given-names>Azeemi</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Hina</surname><given-names>Iram</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Qanita</surname><given-names>Younas</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Ameena</surname><given-names>Azeemi</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib></contrib-group><aff id="aff2"><addr-line>CMH Lahore Medical College, Lahore, Pakistan</addr-line></aff><aff id="aff1"><addr-line>Department of Physics, Government College (W) Gulberg, Lahore, Pakistan</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>saminatazayyen@yahoo.com(SA)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>05</day><month>03</month><year>2018</year></pub-date><volume>09</volume><issue>01</issue><fpage>1</fpage><lpage>6</lpage><history><date date-type="received"><day>3,</day>	<month>January</month>	<year>2018</year></date><date date-type="rev-recd"><day>3,</day>	<month>March</month>	<year>2018</year>	</date><date date-type="accepted"><day>6,</day>	<month>March</month>	<year>2018</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
  The Biological effect of blue color therapy on self-reported anxiety in college students was determined. Female students (n = 150) of age 18 - 24 from a Government college (w) Gulberg in Lahore, Pakistan were randomized into two groups (n = 75 each), Group A (chromotherapy) and Group B (control). For the treatment of anxiety, two methods of chromo therapy used were Hydrochromotherapy (Blue chromotized water) and through watching colors (Blue chart). After 2, 4 and 6 weeks, the number of students experiencing improvement in their anxiety from Group A were 60, 9 and 0 respectively. In Group B, students that recovered from anxiety were 17, 12 and 7 after 2, 4 and 6 weeks respectively. Relation between therapy and improvement in anxiety was examined using 
  χ
  <sup>2</sup>
   statistic. Relation between these variables was significant, (1, n = 150) = 11.24, 
  p
   &lt; 0.01. After this study, 453 nm (blue color) was proved to be an effective intervention for anxiety in students. It confers a greater improvement in anxiety than does no treatment (control). This is cost effective yet an effective way of treatment with no apparent side effects.
 
</p></abstract><kwd-group><kwd>Color Therapy</kwd><kwd> Electromagnetic Radiation</kwd><kwd> Randomized Controlled Trial</kwd><kwd> Anxiety</kwd><kwd> Students</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>University and college counseling centers, during the last few years, have described a change in the needs of students looking for counseling services, from more informational needs, to more serious psychological issues [<xref ref-type="bibr" rid="scirp.82826-ref1">1</xref>] . The researchers e.g. Offer and Spiro [<xref ref-type="bibr" rid="scirp.82826-ref2">2</xref>] concluded that more than 30% of the university students had a psychological problem and 25% of students seeking admission in college were disturbed and required psychological healthcare services. Psychological problems impacting college and university students have short and long-term consequences including decreased work capacity and declined academic performance [<xref ref-type="bibr" rid="scirp.82826-ref3">3</xref>] .</p><p>Chromotherapy, an emerging technique, uses the visible spectrum (colors) of electromagnetic radiations to cure ailments [<xref ref-type="bibr" rid="scirp.82826-ref4">4</xref>] . In the past few years, Colour Therapy has emerged as a therapeutic option for the treatment of various psychological problems and Psychiatric Illnesses [<xref ref-type="bibr" rid="scirp.82826-ref5">5</xref>] [<xref ref-type="bibr" rid="scirp.82826-ref6">6</xref>] . It has been also regarded as an effective intervention in Insomnia [<xref ref-type="bibr" rid="scirp.82826-ref7">7</xref>] , and has been known to affect Circadian Rhythms [<xref ref-type="bibr" rid="scirp.82826-ref8">8</xref>] .</p><p>There is a lack of effective therapeutics for treating mild to moderate anxiety experienced by a large proportion of college students. If the effectiveness of chromotherapy in psychological problems experienced by the students could be justified, then this could provide an alternative treatment, alongside psychological interventions.</p><p>The primary aim of the study was to determine whether Colour therapy treatment produces a greater improvement on self-reported anxiety than does usual supportive care. Chromotherapy was adopted for this study because it has been reported with no side effects by the doctrine of chromotherapy [<xref ref-type="bibr" rid="scirp.82826-ref9">9</xref>] .</p><p>The results of the present study might prove helpful and of importance to both health professionals and the scientific community, along with improving our understanding of colours as therapeutic agents in psychiatric illnesses.</p></sec><sec id="s2"><title>2. Materials and Methods</title><p>The two primary procedures for Colour Therapy are:</p><p>1) Direct exposure to colored light</p><p>2) Hydrochromotherapy</p><p>Hydrochromotherapy is the method that involves irradiation of water through Visible Range Electromagnetic Radiations to produce color absorbed charged water [<xref ref-type="bibr" rid="scirp.82826-ref10">10</xref>] [<xref ref-type="bibr" rid="scirp.82826-ref11">11</xref>] [<xref ref-type="bibr" rid="scirp.82826-ref12">12</xref>] [<xref ref-type="bibr" rid="scirp.82826-ref13">13</xref>] . Both of these methodologies were adopted for the study.</p><sec id="s2_1"><title>2.1. Sample Preparation for Hydrochromotherapy</title><p>The whole procedure for preparing chromotized water was as follows: Transparent glass bottles each with 1 litre capacity were wrapped with 453.5 nm (Blue) filter sheets (Purity: 52%; Transmission: 52%). 750 ml of distilled water was filled in the bottles. On a piece of wood (non-conducting surface), these bottles were placed in sunlight for 6 hours to facilitate the preparation process. Methodology was adopted from [<xref ref-type="bibr" rid="scirp.82826-ref10">10</xref>] [<xref ref-type="bibr" rid="scirp.82826-ref13">13</xref>] .</p></sec><sec id="s2_2"><title>2.2. Direct Exposure</title><p>A glass sheet of 9 &#215; 12 inches was painted with 453 nm blue glass paint. The students were advised to watch this glass sheet for 15 - 20 min before going to bed daily [<xref ref-type="bibr" rid="scirp.82826-ref10">10</xref>] .</p></sec><sec id="s2_3"><title>2.3. Patients and Study Design</title><p>150 female students of age 18 - 24 from a Government college (w) Gulberg in Lahore, Pakistan were chosen for the study with the consent of their family, with study conducted from 1<sup>st</sup> March, 2017 to 12<sup>th</sup> April, 2017.</p><p>Study inclusion criteria: Symptoms of anxiety and depression were elicited using a survey in the college for self-reported anxiety. 210 potential students were identified as borderline, or non-case of anxiety. Out these 210 students, 150 were selected for the study using simple random sampling. The socio-demographic characteristics of these students are shown in <xref ref-type="table" rid="table1">Table 1</xref>. These students were suffering from border-line anxiety. Borderline-case anxiety is equivalent to which might benefit from counseling or specific psychological interventions, such as anxiety management.</p><p>The students suffered from anxiety symptoms, including insomnia, palpitations, cold hands and feet and feelings of panic. The patients were randomised into two groups in 1:1; (n = 75) to the students with anxiety (Group A) or to the control (Group B), (n = 75). The simple randomisation was done using a generated list of random numbers by a computer software.</p><p>In this situation, these groups were treated with 453 nm visible range radiation (blue color). In this case the patients of Group (A) were advised to take 90 ml (3 oz.) of blue (453 nm) chromotized water thrice a day one hour after each meal for six weeks. Along with this they were advised to watch blue glass sheets for 15 to 20 minutes before sleeping and to continue this therapy for 6 weeks in order to get good benefits. This therapy was limited to blue color only because blue color has been proved to control the disturbance of mental activity [<xref ref-type="bibr" rid="scirp.82826-ref7">7</xref>] [<xref ref-type="bibr" rid="scirp.82826-ref10">10</xref>] . Six weeks follow up was observed.</p><p>The control group of students did not take any other form of treatment for their anxiety.</p></sec></sec><sec id="s3"><title>3. Results</title><p>After two weeks, 60 patients experienced improvement in their self-reported anxiety from full case to below borderline in Group A in contrast to 20 patients in Group B. More patients who received chromotherapy showed an improvement than those who did not. Difference in mean improvement after two weeks was 3.48 (80% v 23%).</p><p>After four weeks of treatment, 9 students of Group A experienced improvement</p><table-wrap id="table1" ><label><xref ref-type="table" rid="table1">Table 1</xref></label><caption><title> Socio-demographic characteristics of patient sample (n = 150)</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Independent Variables</th><th align="center" valign="middle" >Group A (n = 75)</th><th align="center" valign="middle" >Group B (n = 75)</th></tr></thead><tr><td align="center" valign="middle" >Average Household Income (1 = below average, 2 = average, 3 = above average)</td><td align="center" valign="middle" >1</td><td align="center" valign="middle" >1</td></tr><tr><td align="center" valign="middle" >Health Status (1 = very good, 2 = good, 3 = fair, 4 = poor)</td><td align="center" valign="middle" >3</td><td align="center" valign="middle" >3</td></tr></tbody></table></table-wrap><p>in self-reported anxiety as compared to 12 patients in Group B (2.86; 60% v 22%).</p><p>After six Weeks, no patients had improvement in their anxiety in Group A (6 of them remained the same) compared to 7 in Group B (0; 0% v 15.2%).</p><p>At the end of 6 weeks, the relation between the therapy and the improvement in anxiety was examined using the χ<sup>2</sup> statistic using the cumulated data. The relation between these variables was significant, (1, n = 150) = 11.24, p &lt; 0.01. i.e. Chromotherapy confers a greater improvement in anxiety than does no care. Difference in mean improvement at the end of study (after 6 weeks) was 1.92; 95% CI, p &lt; 0.01. No recurrence was found after six-week follow-up.</p><p>Daily sessions of chromotherapy improved self-reported anxiety in college students, relieving symptoms of insomnia, headache and palpitations, with 80% of them recovering in the first 2 weeks of treatment (<xref ref-type="fig" rid="fig1">Figure 1</xref>). The patients in the control group experienced late recovery as compared to those given chromotherapy. As the results show, the difference in the improvement of self-reported anxiety between the Chromotherapy and the control even in this limited sample size was clear and persistent (<xref ref-type="table" rid="table2">Table 2</xref>).</p></sec><sec id="s4"><title>4. Discussion</title><p>The doctrine of Colour therapy explains that visible electromagnetic radiations are the generators of electrical impulses and magnetic currents or fields of energy that are prime activators of the biochemical and hormonal processes [<xref ref-type="bibr" rid="scirp.82826-ref4">4</xref>] . Chromotherapy is based on the effect of visible range electromagnetic radiations on human neurohormonal pathways, precisely on melatonin and serotonin pathways in brain [<xref ref-type="bibr" rid="scirp.82826-ref5">5</xref>] [<xref ref-type="bibr" rid="scirp.82826-ref14">14</xref>] . Previous researches have concluded that certain parts of brain are light sensitive and respond differently to different colors. The mechanism depends on the stimulation of deep brain structures namely hypothalamus, pituitary and pineal gland by transduction of electromagnetic field trough</p><table-wrap id="table2" ><label><xref ref-type="table" rid="table2">Table 2</xref></label><caption><title> Effects of Colour Therapy on Self-reported anxiety</title></caption><table><tbody><thead><tr><th align="center" valign="middle" ></th><th align="center" valign="middle" >Total (n = 150)</th><th align="center" valign="middle" >Randomized to Colour Therapy (n = 75)</th><th align="center" valign="middle" >Randomized to Control (n = 75)</th></tr></thead><tr><td align="center" valign="middle" >χ<sup>2</sup> statistic: p &lt; 0.01</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Anxiety only</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Outcome at 2 weeks,%</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Worse</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >0</td></tr><tr><td align="center" valign="middle" >Same</td><td align="center" valign="middle" >70</td><td align="center" valign="middle" >20</td><td align="center" valign="middle" >77</td></tr><tr><td align="center" valign="middle" >Improved</td><td align="center" valign="middle" >80</td><td align="center" valign="middle" >80</td><td align="center" valign="middle" >23</td></tr><tr><td align="center" valign="middle" >Outcome at 4 weeks, %</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Worse</td><td align="center" valign="middle" >7</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >19</td></tr><tr><td align="center" valign="middle" >Same</td><td align="center" valign="middle" >33</td><td align="center" valign="middle" >40</td><td align="center" valign="middle" >60</td></tr><tr><td align="center" valign="middle" >Improved</td><td align="center" valign="middle" >30</td><td align="center" valign="middle" >60</td><td align="center" valign="middle" >21</td></tr><tr><td align="center" valign="middle" >Outcome at 6 weeks, %</td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td><td align="center" valign="middle" ></td></tr><tr><td align="center" valign="middle" >Worse</td><td align="center" valign="middle" >7</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >30</td></tr><tr><td align="center" valign="middle" >Same</td><td align="center" valign="middle" >15</td><td align="center" valign="middle" >100</td><td align="center" valign="middle" >54</td></tr><tr><td align="center" valign="middle" >Improved</td><td align="center" valign="middle" >18</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >15</td></tr></tbody></table></table-wrap><p>the visual pathway via retino-hypothalamic tract [<xref ref-type="bibr" rid="scirp.82826-ref14">14</xref>] .</p><p>Several researches have demonstrated the profound effect of colors on human psychological processes such as behavior and mood [<xref ref-type="bibr" rid="scirp.82826-ref15">15</xref>] e.g. pink color has been shown to have a calming effect on the behavior of prisoners in the American penal system as it suppresses violent and aggressive behavior among inmates [<xref ref-type="bibr" rid="scirp.82826-ref16">16</xref>] . Chromotherapy is an effective, cost-effective and a safe alternative of treating anxiety (with no side-effects); It was thus adopted for this study.</p><p>Color therapy is the rationale therapy and has emerged with a serious scientific background for maintaining health and overcoming disease [<xref ref-type="bibr" rid="scirp.82826-ref6">6</xref>] . Different enzymes in each organ respond to particular wavelength in the visible region [<xref ref-type="bibr" rid="scirp.82826-ref17">17</xref>] . Each color influences us in different aspects. By learning how each color affects us, we can effectively use color to exceed energy blockage [<xref ref-type="bibr" rid="scirp.82826-ref10">10</xref>] .</p></sec><sec id="s5"><title>5. Conclusion</title><p>453 nm (blue color) has proved to be an effective intervention for anxiety in students. It confers a greater improvement in anxiety than does no treatment (control).</p></sec><sec id="s6"><title>Cite this paper</title><p>Azeemi, S., Iram, H., Younas, Q. and Azeemi, A. (2018) Effect of Blue Colour (453 nm Visible Range Radiation) on Anxiety in College Students. Chinese Medicine, 9, 1-6. https://doi.org/10.4236/cm.2018.91001</p></sec></body><back><ref-list><title>References</title><ref id="scirp.82826-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">Hunt, J. and Eisenberg, D. (2010) Mental Health Problems and Help-Seeking Behavior among College Students. Journal of Adolescent Health, 46, 3-10.  
https://doi.org/10.1016/j.jadohealth.2009.08.008</mixed-citation></ref><ref id="scirp.82826-ref2"><label>2</label><mixed-citation publication-type="other" xlink:type="simple">Offer, D. and Spiro, R.P. (1987) The Disturbed Adolescent Goes to College. Journal of American College Health, 35, 209-214.  
https://doi.org/10.1080/07448481.1987.9940443</mixed-citation></ref><ref id="scirp.82826-ref3"><label>3</label><mixed-citation publication-type="other" xlink:type="simple">Stallman, H.M. (2010) Psychological Distress in University Students: A Comparison with General Population Data. Australian Psychologist, 45, 249-257.  
https://doi.org/10.1080/00050067.2010.482109</mixed-citation></ref><ref id="scirp.82826-ref4"><label>4</label><mixed-citation publication-type="other" xlink:type="simple">Azeemi, S.T. and Raza, S.M. (2005) A Critical Analysis of Chromotherapy and Its Scientific Evolution. Evidence-Based Complementary and Alternative Medicine, 2, 481-488. https://doi.org/10.1093/ecam/neh137</mixed-citation></ref><ref id="scirp.82826-ref5"><label>5</label><mixed-citation publication-type="other" xlink:type="simple">Radeljak, S., Zarkovic-Palijan, T., Kovacevic, D. and Kovac, M. (2008) Chromotherapy in the Regulation of Neurohormonal Balance in Human Brain—Complementary Application in Modern Psychiatric Treatment. Collegium Antropologicum, 32, 185-188.</mixed-citation></ref><ref id="scirp.82826-ref6"><label>6</label><mixed-citation publication-type="other" xlink:type="simple">Benchea, A., Babusca, D. and Morariu, M. (2015) Color in Alternative Therapy. FIZICA. SI. TEHNICA., 1, 50-61.</mixed-citation></ref><ref id="scirp.82826-ref7"><label>7</label><mixed-citation publication-type="other" xlink:type="simple">Yousuf, R., Azeemi, S.T.Y. and Rashid, A. (2013) Treatment of Insomnia by Turquoise Colour (495 nm) during Pregnancy. Pakistan Postgraduate Medical Journal, 24, 67-69.</mixed-citation></ref><ref id="scirp.82826-ref8"><label>8</label><mixed-citation publication-type="other" xlink:type="simple">Edelhauser, F., Hak, F., Kleinrath, U., Lühr, B., Matthiessen, P.F., Weinzirl, J. and Cysarz, D. (2013) Impact of Colored Light on Cardiorespiratory Coordination. Evidence-Based Complementary and Alternative Medicine, 2013, Article ID 810876.  
https://doi.org/10.1155/2013/810876</mixed-citation></ref><ref id="scirp.82826-ref9"><label>9</label><mixed-citation publication-type="other" xlink:type="simple">Azeemi, S.T., Mohsin Raza, S. and Yasinzai, M. (2011) A Case History of Treatment of Cutaneous Leishmaniasis by Visible Range Radiation Therapy. Chinese Medicine, 2, 4.</mixed-citation></ref><ref id="scirp.82826-ref10"><label>10</label><mixed-citation publication-type="other" xlink:type="simple">Azeemi, K.S. (1999) Colour Therapy. Al-Kitab Publications, Karachi.</mixed-citation></ref><ref id="scirp.82826-ref11"><label>11</label><mixed-citation publication-type="other" xlink:type="simple">Klotsche, C. (1993) Colour Medicine. Light Technology Publishing, Arizona.</mixed-citation></ref><ref id="scirp.82826-ref12"><label>12</label><mixed-citation publication-type="other" xlink:type="simple">Azeemi, S.T., S. Mohsin Raza, M.Y. and Mujeeb-ur-Rehman (2008) Absorption of Radiant Energy in Water—A New Conjecture and Theory of Charge Quantization in Chromotized Water Samples. Science International, 20, 7.</mixed-citation></ref><ref id="scirp.82826-ref13"><label>13</label><mixed-citation publication-type="other" xlink:type="simple">Azeemi, S.T.Y., Raza, S.M. and Ahmed, M.A. (2008) Newly Developed Recursive Relationship for Fractional Quantum States and Associated Energy Eigenvalues. Science International, 20, 255-260.</mixed-citation></ref><ref id="scirp.82826-ref14"><label>14</label><mixed-citation publication-type="other" xlink:type="simple">Birren, F. (2016) Color Psychology and Color Therapy; A Factual Study of the Influence of Color on Human Life. Pickle Partners Publishing, Chicago, IL.</mixed-citation></ref><ref id="scirp.82826-ref15"><label>15</label><mixed-citation publication-type="other" xlink:type="simple">Elliot, A.J. and Maier, M.A. (2014) Color Psychology: Effects of Perceiving Color on Psychological Functioning in Humans. Annual Review of Psychology, 65, 95-120.  
https://doi.org/10.1146/annurev-psych-010213-115035</mixed-citation></ref><ref id="scirp.82826-ref16"><label>16</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>Schauss</surname><given-names> A.G. </given-names></name>,<etal>et al</etal>. (<year>1979</year>)<article-title>Tranquilizing Effect of Color Reduces Aggressive Behavior and Potential Violence</article-title><source> Journal of Orthomolecular Psychiatry</source><volume> 8</volume>,<fpage> 218</fpage>-<lpage>221</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.82826-ref17"><label>17</label><mixed-citation publication-type="other" xlink:type="simple">Azeemi, S.T., Raza, S.M. and Yasinzai, M. (2008) Colors as Catalysts in Enzymatic Reactions. Journal of Acupuncture and Meridian Studies, 1, 139-142.  
https://doi.org/10.1016/S2005-2901(09)60034-0</mixed-citation></ref></ref-list></back></article>