<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">OJSS</journal-id><journal-title-group><journal-title>Open Journal of Soil Science</journal-title></journal-title-group><issn pub-type="epub">2162-5360</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/ojss.2018.82007</article-id><article-id pub-id-type="publisher-id">OJSS-82659</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Earth&amp;Environmental Sciences</subject></subj-group></article-categories><title-group><article-title>
 
 
  Effect of Different Levels of Nitrogen on Yield of Colocasia (&lt;i&gt;Colocasia esculenta&lt;/i&gt;) at District Malakand Dargai
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Rafiq</surname><given-names>Ahmad</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Dost</surname><given-names>Muhammad</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Maria</surname><given-names>Mussarat</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Shah</surname><given-names>Fahad</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Shahid</surname><given-names>Ullah</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Taimur</surname><given-names>Ahmad</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Sara</surname><given-names>Wahab</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib></contrib-group><aff id="aff4"><addr-line>Department of Horticulture, AMK Campus Mardan, The University of Agriculture Peshawar, Peshawar, Pakistan</addr-line></aff><aff id="aff3"><addr-line>Department of Agronomy, The University of Agriculture, Peshawar, Pakistan</addr-line></aff><aff id="aff1"><addr-line>Department of Soil and Environmental Sciences, The University of Agriculture, Peshawar, Pakistan</addr-line></aff><aff id="aff2"><addr-line>University of Swabi, Swabi, Pakistan</addr-line></aff><aff id="aff5"><addr-line>Department of Zoology, Abdul Wali Khan University Mardan, Mardan, Pakistan</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>rafiqsoil5454@yahoo.com(RA)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>12</day><month>02</month><year>2018</year></pub-date><volume>08</volume><issue>02</issue><fpage>87</fpage><lpage>98</lpage><history><date date-type="received"><day>7,</day>	<month>December</month>	<year>2017</year></date><date date-type="rev-recd"><day>24,</day>	<month>February</month>	<year>2018</year>	</date><date date-type="accepted"><day>27,</day>	<month>February</month>	<year>2018</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
  A field experiment was conducted to evaluate the response of colocasia (
  Colocasia esculenta) to different levels of 0, 60, 90, 120 and 150 kg N ha
  <sup>-1</sup> under farmer’s field condition at Garhi Usmani Khel, District Malakand Dargai during 2013. The experiment was laid out in Randomized Complete Block design with three replications and treatment plot size of 2.74 &#215; 2.43 m
  <sup>2</sup>. All levels of N in the form of urea along with uniform basal doze of 90 kg P
  <sub>2</sub>O
  <sub>5</sub> ha
  <sup>-1</sup> as Triple Super Phosphate (TSPPP) were applied to soil at time of seed bed followed by thorough mixing. Seeds of colocasia c.v. local variety were sown in these plots with row spacing of 30 cm and plant to plant distance of 12 cm in February, 2013. The results showed that application of N produced significantly higher colocasia tuber yield, number of tubers plant
  <sup>-1</sup>, 1000-tubers weight and size of tubers (mean length and diameter) over control but the differences among levels of N were nonsignificant. However, some parameters like tuber yield was maximum at 60 kg N ha
  <sup>-1</sup> and tuber size especially the length of colocasia tuber was maximum at 150 kg N ha
  <sup>-1</sup> suggesting that the response of each parameter was different to N levels. Based on maximum relative yield (100%) and increase over control (46.1%) still at lower N levels of 60 kg N ha
  <sup>-1</sup>, this level seems to be appropriate level for colocasia under the prevailing soil and climatic conditions.
 
</p></abstract><kwd-group><kwd>&lt;i&gt;Colocasia esculenta&lt;/i&gt;</kwd><kwd> Nitrogen</kwd><kwd> Tuber Yield</kwd><kwd> Agronomic Character</kwd><kwd> Pakistan</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>Colocasia (Colocasia esculenta) having about 25 species is a group of flowering plants widely used for food and landscaping purposes [<xref ref-type="bibr" rid="scirp.82659-ref1">1</xref>] . Leaves of these plants are shaped like shields or arrow head each growing on a single stem and ranging from 25 to 150 cm across. The large floppy leaves of colocasia gave the plant a common name, elephant ear. In the garden they are prized as ornamental plants growing as a focal point in the back of a landscaping plan [<xref ref-type="bibr" rid="scirp.82659-ref2">2</xref>] . Several varieties produce different plant sizes, leaf shape, and different colors ranging from bright green through reddish-tinged and black-outlined foliage. Colocasia grows best in tropical areas with plentiful water. When the plants dry out, the leaves fold, droop and will develop dry brown spots or edges after a drought. The plant can be cultivated by planting the tubers or rhizomes just below the surface or mature plants can be set into pre-watered holes.</p><p>As a food plant, colocasia is used throughout Africa, the Caribbean and South America, and as well as in Asia and Pakistan. Nowadays, it is considered the fifth most consumed root vegetable worldwide [<xref ref-type="bibr" rid="scirp.82659-ref3">3</xref>] . The edible varieties are called taro, cocoyam, dasheen or eddoe. Taro is one of the few major staple foods where both the leaf and underground parts are important in the human diet [<xref ref-type="bibr" rid="scirp.82659-ref4">4</xref>] . The tubers of the plant are white-fleshed with a dark-colored bark that is peeled before eating and the flavor is somewhat similar to a potato. The corms supply easily digestible starch and are known to contain substantial amounts of protein, vitamin C, thiamine, riboflavin, niacin and significant amounts of dietary fiber [<xref ref-type="bibr" rid="scirp.82659-ref5">5</xref>] . The leaves have a taste similar to spinach or collards. The leaves are high in minerals and vitamins A, C, K, Ca, P, Fe, thiamine and niacin [<xref ref-type="bibr" rid="scirp.82659-ref6">6</xref>] . The Caribbean dish callaloo, a stew with variable ingredients always contains the chopped leaves of the colocasia plant and sometimes also includes the chopped tubers. Some South American cultures dry the tubers and pound them into flour to be used in baking and as a thickening agent. Harvesting takes place when the plants are 7 - 8 months old or when only three functional leaves remain on the plant. Yields are estimated to range from 4 - 7 tons of marketable corms (tubers) per hectare [<xref ref-type="bibr" rid="scirp.82659-ref7">7</xref>] .</p><p>Like other agricultural and ornamental plants, balanced nutritional supply play the key role in production of colocasia. Among these nutrients, the nitrogen which is required in higher amounts by plants and is often deficient in most areas is relative more responsive. The farmers and growers all over the world primarily focus on nitrogen application before any other nutrient. Though nitrogen is an abundant element on and around Earth―approximately 78 percent of the earth’s atmosphere is nitrogen gas [<xref ref-type="bibr" rid="scirp.82659-ref8">8</xref>] but it is not available to plant in this form. It is frequently deficient in soils including the tropical soils as well [<xref ref-type="bibr" rid="scirp.82659-ref9">9</xref>] . Nitrogen has numerous functions in the plant. Many plant enzymes are proteinaceous and hence nitrogen play a key role in many metabolic reactions of plant and adequate N promotes vigorous vegetative growth and impart deep green color. In cereals crops it increase the plumpness of a grains. Nitrogen is also the part of chlorophyll molecule so a deficiency of nitrogen will result in a chlorotic condition of the plant which decreases the quality of its fruits. Plant root absorb N in the form of a NO 3 − or NH 4 + ions. Urea (NH<sub>2</sub>CONH<sub>2</sub>) can also be absorbed by plant leaves when applied in solution form as a foliar application. In this form N is directly and rapidly absorbed through the leaf epidermis. In colocasia as well, the application of N significantly improve its yield and yield components [<xref ref-type="bibr" rid="scirp.82659-ref10">10</xref>] . However, the optimum level of N has not been extensively studied and probably not at all in local conditions of the province. This study was initiated to evaluate the optimum level of N required for higher yield of colocasia under the local conditions of Malakand Dargai. Keeping in view the importance of nitrogen for enhancing the yield of colocasia, the present study was therefore, designed to evaluate the effect of different levels of N on its yield and to select the best level of N for higher yield and yield components of colocasia under agro climatic condition of village Garhi Usmani khel Dargai Malakand.</p></sec><sec id="s2"><title>2. Materials and methods</title><sec id="s2_1"><title>2.1. Field experimentation</title><p>An experiment was conducted to determine the effect of different levels of nitrogen on the yield and yield components of colocasia at District Malakand village Garhi Usmani Khel during 2013. The study was consisted of five level of nitrogen 0, 60, 90, 120 and 150 kg・ha<sup>−1</sup>. The experiment was conducted in randomized complete block design with three replications having plot size 2.74 m &#215; 2.43 m. Local colocasia variety was sown on a well prepared clay soil. The spacing between plant to plant 12 cm and row to row was 30 cm. In each row 12 colocasia tubers were planted. The entire quantity of nitrogen in the form of urea was applied to the respective plots at seed bed preparation. The crop was sown in February and then harvested in October. Data on number of colocasia plot<sup>−1</sup>, 1000-tubers weight, length, diameter and total yield of colocasia was recorded. Manual weeding was performed three times in the cropping season. After 3 months of the emergence of colocasia ridges were performed for better production of colocasia tubers.</p><p>During month of May fungal disease called blight of colocasia appeared and was controlled by a fungicide Metalixal + Mancozeb 72% wp@ 3 gm per liter three times at the interval of 10 days the whole month. In month of June crop was again attacked by insects (worms) and Chlorpyrifos 40 ec + Acetamiprid 20% sp insecticide was used at 3 gm per 10 liter. A high rainfall in the month of august caused a severe brownish spots on leaves that was a fungal attack. Metalixal + Mancozeb 72% wp@ 3 gm per liter and Acetamiprid 20% sp of 3 gm per 10 liter was used 3 time at 10 days interval the whole month. The colocasia was harvested at the month of October. All other agronomic practices such as irrigation and plant protection measures etc were kept normal and uniform for all the experimental units. Data on tuber yield (kg・ha<sup>−1</sup>), number of tuber per plant, weight of tubers and size of tuber were recorded.</p></sec><sec id="s2_2"><title>2.2. Statistical Analysis</title><p>The data was analyzed, thus Analysis of variance (ANOVA) procedure for randomized complete design by a statistic 8.1 software. The variations between the treatments were compared by LSD test of significance [<xref ref-type="bibr" rid="scirp.82659-ref11">11</xref>] .</p></sec></sec><sec id="s3"><title>3. Laboratory Analysis</title><p>Four composite soil samples were collected and passes through 2 mm sieve to separate roots and plant stubbles. Soil samples were analyzed for physical and chemical properties of soil such as soil texture, P<sup>H</sup>, EC, OM, AB-DTPA extractable Phosphorus (P) and total N as shown in table 1.</p><sec id="s3_1"><title>3.1. Soil texture</title><p>The Hydrometer was used to investigate texture of soil as presented by [<xref ref-type="bibr" rid="scirp.82659-ref12">12</xref>] . Take 50 g soil and then added 10 ml of Na<sub>2</sub>CO<sub>3</sub> and water into a dispersion cup. Shake for 5 minutes through dispersing machine to break up all the aggregates. Reading was noted in hydrometer after 40 second and after 2 hours along with the temperature. First reading mean after 40 second represent silt and clay while 2 hour reading shows clay in the suspension.</p></sec><sec id="s3_2"><title>3.2. Soil pH</title><p>Ten grams of soil was added to 50 ml of distilled water and then shake for 20 - 30 minutes to make a 1:5 suspension. The pH was determined in the suspension using pH meter (Inolab WTW Series pH 720) after calibrating the instrument with standard buffers of 4.0 and 10.0.</p></sec><sec id="s3_3"><title>3.3. Soil EC</title><p>The electrical conductivity was calculated in soil suspensions of 1:5 by using EC meter after calibration with KCL standard solution as presented by [<xref ref-type="bibr" rid="scirp.82659-ref13">13</xref>] .</p></sec><sec id="s3_4"><title>3.4. Organic Matter</title><p>Organic matter was calculated by [<xref ref-type="bibr" rid="scirp.82659-ref14">14</xref>] . 1 gm of dried soil was taken in a conical</p><table-wrap id="table1" ><label><xref ref-type="table" rid="table1">Table 1</xref></label><caption><title> Experimental field pre-harvest soil physico-chemical properties</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Properties</th><th align="center" valign="middle" >Units</th><th align="center" valign="middle" >Values</th></tr></thead><tr><td align="center" valign="middle" >Clay</td><td align="center" valign="middle" >%</td><td align="center" valign="middle" >13.8</td></tr><tr><td align="center" valign="middle" >Silt</td><td align="center" valign="middle" >&quot;</td><td align="center" valign="middle" >30</td></tr><tr><td align="center" valign="middle" >Sand</td><td align="center" valign="middle" >&quot;</td><td align="center" valign="middle" >56.2</td></tr><tr><td align="center" valign="middle" >Textural Class</td><td align="center" valign="middle" >-</td><td align="center" valign="middle" >Sandy loam</td></tr><tr><td align="center" valign="middle" >pH<sub>(1:5)</sub></td><td align="center" valign="middle" >-</td><td align="center" valign="middle" >7.15</td></tr><tr><td align="center" valign="middle" >EC<sub>(1:5)</sub></td><td align="center" valign="middle" >dSm<sup>−1</sup></td><td align="center" valign="middle" >0.13</td></tr><tr><td align="center" valign="middle" >OM (%)</td><td align="center" valign="middle" >%</td><td align="center" valign="middle" >0.99</td></tr><tr><td align="center" valign="middle" >Extractable (AB-DTPA) P</td><td align="center" valign="middle" >mg・kg<sup>−1</sup></td><td align="center" valign="middle" >2.02</td></tr><tr><td align="center" valign="middle" >Total N</td><td align="center" valign="middle" >%</td><td align="center" valign="middle" >0.113</td></tr></tbody></table></table-wrap><p>flask along with 10 ml of 0.5 N K<sub>2</sub>Cr<sub>2</sub>O<sub>7</sub> and 20 ml of conc. H<sub>2</sub>SO<sub>4</sub>. It was then allowed to stand for 30 min to complete the reaction. After sometime add 200 ml of distilled water and the suspension was filtered. Then 2 - 3 drops of Ortho phenolphthalein indicator was added to the filtrate. It was then titrated against 0.5 N Fe<sub>2</sub>SO<sub>4</sub>・7H<sub>2</sub>O until the color changed to dark brown, representing the end point. The percent organic matter was calculated using the following formula.</p><p>%OM= [ ( mLofK 2 Cr 2 O 7 &#215; N ) − ( mLofFeSO 4 ⋅ 7H 2 O &#215; N ) ] Weightofsoil &#215; 0 .69</p></sec><sec id="s3_5"><title>3.5. AB-DTPA Extractable P</title><p>P concentration in soil sample was determined by extracting soil solution with AB-DTPA as presented by [<xref ref-type="bibr" rid="scirp.82659-ref15">15</xref>] . 10 g of soil and 20 ml of AB-DTPA solution was added to a conical flask and placed on shaking machine for 15 minutes. Then the samples were filtered with Watman No. 42. One ml sample along with 5 ml of ascorbic acid was taken and made the volume up to 25 ml. Then with the help of spectrophotometer (Lambda-35), P was determined at 880 nm after proper color development.</p></sec><sec id="s3_6"><title>3.6. Determination of total N in soil</title><p>Kjeldhal method of [<xref ref-type="bibr" rid="scirp.82659-ref16">16</xref>] is used to evaluate total N in soil and plant samples. Finely 0.2 g ground sample of dry materials were digested with 3 ml of concentrated H<sub>2</sub>SO<sub>4</sub> in the presence of 1.1 g digestion mixture containing CuSO<sub>4</sub>, K<sub>2</sub>SO<sub>4</sub> and Se on a heating mantle for 1 hour. The digest was transferred into the distillation flask and distilled in the presence of 10 ml of 10 M NaOH solution. The distillate was then taken in 5 ml boric acid mixed indicator solution and then titrated against 0.01 M HCl. Calculation for total nitrogen in soil are as follow:</p><p>TotalN ( % ) = ( Sample-Blank ) &#215; 0 .005 &#215; 0 .014 &#215; 100 &#215; 100 / Wt .ofsoil &#215; 20nt</p></sec></sec><sec id="s4"><title>4. Results and Discussion</title><sec id="s4_1"><title>4.1. Tuber yield Data</title><p>Data regarding yield of colocasia are given in <xref ref-type="table" rid="table2">Table 2</xref>. Analysis of the data showed that nitrogen levels significantly (P ≤ 0.05) affected yield of colocasia. The highest yield of colocasia 14.15 t・ha<sup>−1</sup> was recorded in the plot treated with 60 kg N ha<sup>−1</sup>, followed by 90 kg N ha<sup>−1</sup> with a yield of 13.59 t・ha<sup>−1</sup> of colocasia. The lowest yield with value of 9.63 t・ha<sup>−1</sup> was observed in control. Calculating the increase in yield, the application of 60 kg N ha<sup>−1</sup> increased the yield by 46.90% over the control (<xref ref-type="table" rid="table2">Table 2</xref>, <xref ref-type="fig" rid="fig1">Figure 1</xref>). However further application of N did not further increase this trend, rather it showed the decreasing trend. Both the increase over control and relative yield (<xref ref-type="fig" rid="fig1">Figure 1</xref>) showed the highest values at 60 kg N ha<sup>−1</sup> revealed that it could be the suitable and more economical level of N for colocasia under the prevailing soil and climatic conditions for the given variety. However, this level of 60 kg N ha<sup>−1</sup> seems much lower than the expected</p><p>and reported values of [<xref ref-type="bibr" rid="scirp.82659-ref17">17</xref>] who used and recommended much higher value of N for economical yield of colocasia and sweet potato. Optimum levels of nitrogen fertilization for tuber yield in colocasia were found to be 79.62 kg N [<xref ref-type="bibr" rid="scirp.82659-ref18">18</xref>] . The result is in contrast with [<xref ref-type="bibr" rid="scirp.82659-ref19">19</xref>] who reported that maximum yield attributes and yield were found with 120 kg N ha<sup>−1</sup>. Similarly, [<xref ref-type="bibr" rid="scirp.82659-ref20">20</xref>] reported that the highest stolon yield (24.5 t・ha<sup>−1</sup>) was recorded with 125 kg N ha<sup>−1</sup> and 125 kg K ha<sup>−1</sup>, which is in agreement with the findings of our study. [<xref ref-type="bibr" rid="scirp.82659-ref21">21</xref>] also observed the highest corm yield with N<sub>120</sub>K<sub>120</sub> kg・ha<sup>−1</sup>. No doubt the N requirements of the crop varies from soil to soil, crop to crop and variety and also the lower N requirement in our condition could be associated to lower yield potential of the crop variety. The local variety (lower yielding) was used in the said study as no higher yielding variety was available at time of sowing in the market.</p></sec><sec id="s4_2"><title>4.2. Tuber weight</title><p>Thousand colocasia weight (kg) showed significant variation (P ≤ 0.05) with N applied. The maximum 1000-colocasia weight of 31.18 kg was recorded in the plot receiving 90 kg N ha<sup>−1</sup>, followed by 120 kg N ha<sup>−1</sup> with a weight of 27.41 kg. The minimum weight of 22.92 kg was observed in control. Calculating the increase in yield, the application of 90 kg N ha<sup>−1</sup> increased the 1000 colocasia weight by 36% over control. (<xref ref-type="table" rid="table3">Table 3</xref>, <xref ref-type="fig" rid="fig2">Figure 2</xref>). Stolon length was increased with the application of more nitrogen [<xref ref-type="bibr" rid="scirp.82659-ref22">22</xref>] which in turn increases the 1000 stolon mass. Meanwhile, [<xref ref-type="bibr" rid="scirp.82659-ref23">23</xref>] reported the maximum number of leaves plant<sup>−1</sup> with 80 kg N ha<sup>−1</sup> and 120 kg K ha<sup>−1</sup> which is the reason for the increase in the 1000 stolon weight. However further application of N did not further increase this trend, rather it showed the decreasing trend. Both the increase over control and relative yield (<xref ref-type="fig" rid="fig2">Figure 2</xref>) showed the highest values at 90 kg N ha<sup>−1</sup> revealed that it could be the suitable and more economical level of N for colocasia under the prevailing soil and climatic conditions for the given variety.</p></sec><sec id="s4_3"><title>4.3. Number of colocasia plant<sup>−1</sup></title><p>The data showed that number of colocasia tubers plant<sup>−1</sup> was significantly (P ≤ 0.05) affected by different levels of nitrogen (<xref ref-type="table" rid="table4">Table 4</xref>). The maximum number 16.63 was observed in treatments receiving 90 kg N ha<sup>−1</sup> followed by 60 kg N ha<sup>−1</sup></p><table-wrap id="table2" ><label><xref ref-type="table" rid="table3">Table 3</xref></label><caption><title> The 1000 number of colocasia as influenced by different levels of nitrogen</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Treatments</th><th align="center" valign="middle" >N levels</th><th align="center" valign="middle" >1000 tuber weight (kg)</th><th align="center" valign="middle" >Increase over control (%)</th><th align="center" valign="middle" >Relative weight (%)</th></tr></thead><tr><td align="center" valign="middle" >T1</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >22.92 c</td><td align="center" valign="middle" >-</td><td align="center" valign="middle" >73.52</td></tr><tr><td align="center" valign="middle" >T2</td><td align="center" valign="middle" >60</td><td align="center" valign="middle" >25.56 b c</td><td align="center" valign="middle" >11.5</td><td align="center" valign="middle" >81.97</td></tr><tr><td align="center" valign="middle" >T3</td><td align="center" valign="middle" >90</td><td align="center" valign="middle" >31.18 a</td><td align="center" valign="middle" >36.0</td><td align="center" valign="middle" >100.00</td></tr><tr><td align="center" valign="middle" >T4</td><td align="center" valign="middle" >120</td><td align="center" valign="middle" >27.41 b</td><td align="center" valign="middle" >19.6</td><td align="center" valign="middle" >87.92</td></tr><tr><td align="center" valign="middle" >T5</td><td align="center" valign="middle" >150</td><td align="center" valign="middle" >26.61 c</td><td align="center" valign="middle" >16.1</td><td align="center" valign="middle" >85.35</td></tr></tbody></table></table-wrap><p>with a number of 16.0 colocasia plant<sup>−1</sup>. The minimum number of 10.99 colocasia tuber plant<sup>−1</sup> was recorded in control. Calculating the increase in number of colocasia plant<sup>−1</sup>, the application of 90 kg N ha<sup>−1</sup> increased the number of colocasia plant<sup>−1</sup> by 51.33% over the control (<xref ref-type="table" rid="table4">Table 4</xref>, <xref ref-type="fig" rid="fig3">Figure 3</xref>). Increase in the colocasia per plant is due to N increased which is associated with vegetative growth of plants [<xref ref-type="bibr" rid="scirp.82659-ref24">24</xref>] . It is also linked with vigorous vegetative growth, photosynthesis, and dark green color 11 pigmentations [<xref ref-type="bibr" rid="scirp.82659-ref25">25</xref>] . Availability of usable N in most agricultural conditions is a major limiting factor of plant growth and in most tropical soils yields of taro may be improved when inorganic N fertilizers are used. Studies conducted by [<xref ref-type="bibr" rid="scirp.82659-ref26">26</xref>] revealed that taro requires relatively high N especially in its early stages of growth. However further application of N did not increase this trend, rather it showed the decreasing trend. Both the % increase over control and relative number of colocasia tuber plant<sup>−1</sup> (<xref ref-type="fig" rid="fig3">Figure 3</xref>) were maximum at 90 kg N ha<sup>−1</sup> revealed that it could be the suitable and more economical level of N for colocasia under the prevailing soil and climatic conditions for the given variety.</p><table-wrap id="table3" ><label><xref ref-type="table" rid="table4">Table 4</xref></label><caption><title> The number of colocasia as influenced by different levels of nitrogen</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Treatments</th><th align="center" valign="middle" >N levels</th><th align="center" valign="middle" >Number No. plant<sup>−1</sup></th><th align="center" valign="middle" >Increase over control (%)</th><th align="center" valign="middle" >Relative number (%)</th></tr></thead><tr><td align="center" valign="middle" >T1</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >10.99 b</td><td align="center" valign="middle" >-</td><td align="center" valign="middle" >66.08</td></tr><tr><td align="center" valign="middle" >T2</td><td align="center" valign="middle" >60</td><td align="center" valign="middle" >16.00 a</td><td align="center" valign="middle" >45.602</td><td align="center" valign="middle" >96.21</td></tr><tr><td align="center" valign="middle" >T3</td><td align="center" valign="middle" >90</td><td align="center" valign="middle" >16.63 a</td><td align="center" valign="middle" >51.335</td><td align="center" valign="middle" >100.00</td></tr><tr><td align="center" valign="middle" >T4</td><td align="center" valign="middle" >120</td><td align="center" valign="middle" >15.80 a</td><td align="center" valign="middle" >43.782</td><td align="center" valign="middle" >95.01</td></tr><tr><td align="center" valign="middle" >T5</td><td align="center" valign="middle" >150</td><td align="center" valign="middle" >15.01 a</td><td align="center" valign="middle" >36.603</td><td align="center" valign="middle" >90.27</td></tr></tbody></table></table-wrap></sec><sec id="s4_4"><title>4.4. Average Size of colocasia</title><p>Both maximum length and maximum diameter of the colocasia increased with increase in N levels (<xref ref-type="table" rid="table5">Table 5</xref>). The highest length of 5.84 cm was obtained in plots treated with 150 kg N ha<sup>−1</sup>, followed by 120 kg N ha<sup>−1</sup> with a length of 5.78 cm. The lowest length noted was 4.97 cm in a control. Calculating the increase in length, the application of 150 kg N ha<sup>−1</sup> increased the length by 17.62% over the 120 kg N ha<sup>−1</sup> (<xref ref-type="table" rid="table5">Table 5</xref>, <xref ref-type="fig" rid="fig4">Figure 4</xref>). Unlike other parameter, the length of tubers of colocasia increased with increase up to the higher level of N application as concluded by [<xref ref-type="bibr" rid="scirp.82659-ref20">20</xref>] , he concluded that maximum stolon length was recorded with application of N at the rate of 125 kg・ha<sup>−1</sup> (<xref ref-type="fig" rid="fig4">Figure 4</xref>). Increase in the stolon length might be due to increase in the photosynthesis as [<xref ref-type="bibr" rid="scirp.82659-ref27">27</xref>] observed the maximum number of leaves plant<sup>−1</sup> of Colocasia esculenta, with 100 kg N ha<sup>−1</sup>. While, [<xref ref-type="bibr" rid="scirp.82659-ref23">23</xref>] reported the maximum number of leaves plant<sup>−1</sup> with 80 kg N ha<sup>−1</sup>.</p><p>Similarly, the maximum mean diameter significantly (P ≤ 0.05) affected by different levels of nitrogen and the maximum mean diameter of 3.14 cm was recorded in plots treated with 90 kg N ha<sup>−1</sup>, followed by 60 kg N ha<sup>−1</sup> with a diameter value of 3.10 cm. The minimum diameter of colocasia tuber was recorded in a control that was 2.66 cm. Comparing the increase in diameter over control, it was observed that application of 90 kg N ha<sup>−1</sup> increased the colocasia diameter</p><table-wrap id="table4" ><label><xref ref-type="table" rid="table5">Table 5</xref></label><caption><title> Length of colocasia as influenced by different levels of nitrogen</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Treatments</th><th align="center" valign="middle" >N levels</th><th align="center" valign="middle" >Max. length (cm)</th><th align="center" valign="middle" >% increase (%)</th><th align="center" valign="middle" >Relative Length (%)</th></tr></thead><tr><td align="center" valign="middle" >T1</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >4.97 b</td><td align="center" valign="middle" >-</td><td align="center" valign="middle" >85.02</td></tr><tr><td align="center" valign="middle" >T2</td><td align="center" valign="middle" >60</td><td align="center" valign="middle" >5.45 a</td><td align="center" valign="middle" >9.636</td><td align="center" valign="middle" >93.21</td></tr><tr><td align="center" valign="middle" >T3</td><td align="center" valign="middle" >90</td><td align="center" valign="middle" >5.72 a</td><td align="center" valign="middle" >15.099</td><td align="center" valign="middle" >97.85</td></tr><tr><td align="center" valign="middle" >T4</td><td align="center" valign="middle" >120</td><td align="center" valign="middle" >5.78 a</td><td align="center" valign="middle" >16.387</td><td align="center" valign="middle" >98.95</td></tr><tr><td align="center" valign="middle" >T5</td><td align="center" valign="middle" >150</td><td align="center" valign="middle" >5.84 a</td><td align="center" valign="middle" >17.622</td><td align="center" valign="middle" >100.00</td></tr></tbody></table></table-wrap><p>by 18.15% and it was maximum among all treatments (<xref ref-type="table" rid="table5">Table 5</xref>, <xref ref-type="fig" rid="fig4">Figure 4</xref>). Some researchers have shown that NPK fertilization enhanced growth and corm yield of taro [<xref ref-type="bibr" rid="scirp.82659-ref28">28</xref>] and [<xref ref-type="bibr" rid="scirp.82659-ref29">29</xref>] . It is known that taro consumes substantial amounts of potassium [<xref ref-type="bibr" rid="scirp.82659-ref30">30</xref>] . N increases the number and length of the internodes and thus results in progressive increase in plant height. Different researchers including [<xref ref-type="bibr" rid="scirp.82659-ref31">31</xref>] [<xref ref-type="bibr" rid="scirp.82659-ref32">32</xref>] [<xref ref-type="bibr" rid="scirp.82659-ref33">33</xref>] made similar observations. However, further application of N did not further increase this trend, rather it showed the decreasing trend though it was not significant reduction. Both the increase over 60 kg N ha<sup>−1</sup> and relative yield (<xref ref-type="fig" rid="fig4">Figure 4</xref>) showed the highest value at 90 kg N ha<sup>−1</sup> which revealed that it could be the suitable and more economical level of N for colocasia under the prevailing soil and climatic conditions for the given variety.</p></sec></sec><sec id="s5"><title>5. Conclusion and Recommendations</title><p>Application of N produced significantly higher colocasia tuber yield and other yielding components than control but the non-significant difference among the N levels reveals that the minimum level of N would be recommended for the given crop cultivar.</p></sec><sec id="s6"><title>Cite this paper</title><p>Ahmad, R., Muhammad, D., Mussarat, M., Fahad, S., Ullah, S., Ahmad, T. and Wahab, S. (2018) Effect of Different Levels of Nitrogen on Yield of Colocasia (Colocasia esculenta) at District Malakand Dargai. Open Journal of Soil Science, 8, 87-98. https://doi.org/10.4236/ojss.2018.82007</p></sec></body><back><ref-list><title>References</title><ref id="scirp.82659-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">Louis, S. (2010) Missouri Botanical Garden “Colocasia Schott subordinate taxa”. Tropicos, Missouri Botanical Garden, St. Louis.</mixed-citation></ref><ref id="scirp.82659-ref2"><label>2</label><mixed-citation publication-type="other" xlink:type="simple">Wirth, F.F., Davis, K.J. and Wilson, S.B. (2004) Florida Nursery Sales and Economic Impacts of 14 Potentially Invasive Landscape Plant Species. Journal of Environmental Horticulture, 22, 12-16.</mixed-citation></ref><ref id="scirp.82659-ref3"><label>3</label><mixed-citation publication-type="book" xlink:type="simple">Rao, V.R.D., Hunter, P.B.E. and Matthews, P.J. (2010) Ethno Botany and Global Diversity of Taro. 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