Since the dawn of civilization, cosmetic products have been considered a part of routine body care [1] [2] . During the past few decades, The use of these products including care creams, talcum and face powders, lipstick, kajal, sindoor, eye makeup, and mouthwash has increased markedly in the last few decades [2] [3] . Dermal exposure is considered the most significant concern because most cosmetic products are direct practice to the skin. Oral exposure can also occur when cosmetics containing heavy metal tarnishes are applied around the mouth and from hand-to-mouth contact [4] . Heavy metals are incorporated into these products for functional reasons. Thus, for instance, the main ingredients of press powder for eye shadow are talc and pigments, with zinc or magnesium stearate used as a binding agent. A brilliant metallic finish is created with copper, aluminum, brass, gold, or silver powders. Heavy metals, such as cadmium, copper, and lead, remain as tarnishes in the pigments used in eye shadows or are released by the machinery used during the industrializing process [5] .

Lead, cadmium, mercury, chromium, nickel, and copper are the most common heavy metals detected in cosmetic products, including shampoo, lipstick, cream, eye shadow and powder [5] . The ingredients and colorants, along with inadequate purification of raw materials, contribute to the presence of these impurities in cosmetics [6] . Cosmetics appear on the list of products manufactured in various parts of the world for which recall notices have been issued in the US. Thus, in Caribbean countries, an import alert was declared for skin-whitening cream after Hg level in the product measure 8% [7] .

Regarding the potential adverse effects of heavy metal (Pb, Cd, Hg) contamination, the widespread availability and use of cosmetic products has attracted the attention of researchers and clinicians [8] [9] [10] . The fact that, when heavy metal ions come into contact with the human body, they are absorbed and form complexes with the carboxylic acids (-COOH), amines (-NH₂), and thiols (-SH) of proteins, results in cell damage, death and/or leads to a variety of diseases. Treatment for metal intoxication usually involves a chelating agent that binds with the metal ions to form complexes that are then removed from the body [11] . Some cosmetics have been implicated in such harmful effects as cancer, allergic reactions, mutations, respiratory distress, and developmental and reproductive problems [12] . Elevated levels of Cd have been reported to interfere with DNA replication and zinc causing symptoms that mimic those of lead poisoning [13] . Table 1 summarizes the poisoning effect of selected heavy metals in the form of various diseases when ingested or inhaled [9] .

The absorption of toxic metals through dermal contact is scanty, thus little data are known regarding personal care products [14] . Arecent literature survey has been conducted in 2004 in US on the use of lipstick [15] . Over 63% of girls aged 7 - 19 were used this product. Lipstick is ingested when the lips are licked during eating, drinking, or kissing, thus it is said that “women without intention eat about 4 lb of lipsticks in a lifetime” [15] . Few current international standards

are for heavy metal impurities in cosmetics, apart from limits of 20 μg/g Pb and 5 μg/g for Cd [2] . The Canadian government has imposed limits for certain metals in cosmetics: 10 μg/g for Pb, 3 μg/g for As, Cd and Hg, and 5 μg/g for Sb [16] . The levels of Ni, Cr, and Co should not exceed 170 μg/g⁻¹whereas Pb should be within 20 μg/g⁻¹ [17] . The European Union (EU) has also bump up a list of more than 1000 compounds that are not permitted from use in cosmetic manufacturing [7] . On the other hand, Directive 76/768/EEC has banned the use of Cd, Co, Cr, Ni, and Pb in the cosmetics preparation [18] .

The Saudi Standards, Metrology, and Quality Organization (SASO//1953/ 2005) have banned many metals, including arsenic compounds, barium and its salts, Be, Cd and their compounds, Cr, Au salts, phosphorus compounds, potassium cyanide, iodine, lead and its compounds, and compounds containing strontium and selenium, and placed limits on titanium oxide [19] . Thus, the overall goals of the present study are focused on: 1) Ascertaining the concentrations of heavy metals in various local and imported cosmetics; 2) Helping the consumers in seeking more healthy alternatives to products that contain these impurities and finally; 3) producers that will bring to market cosmetics that do not compromise human health under optimized conditions.

Twenty-two lipstick products purchased from a market in Jeddah were selected for analysis, twelve light- and ten dark-colored. An analytical estimation test was performed on fourteen elements in the lipsticks as summarized in (Table 4). The data presented in Table 4 reveal a marked difference from the data reported in the literature (Table 1). Comparing the results inside each class, it is clear that

the Al, Fe, Ba, and Zn concentrations in the samples under examination are relatively higher than those of the other elements analyzed (Figure 1). The (n = 22) average concentrations of heavy metals in the tested samples (22) were found equal 313.18 ± 0.09 μg/g (range 201 - 11795 μg/g), with the highest concentration in (3-A) whereas the lowest in (4-B) was noticed for Al; 9642.92 ± 0.079 μg/g (69 - 59702 μg/g). Regarding Fe, the highest concentration in (2-B) and minimum in (10-A) and the overall value was 46.59 ± 0.109 μg/g (16 - 187 μg/g). The highest concentration observed in (1-B) followed by the lowest concentration in (8-B) was noticed for Ti; 0.545 ± 0.009 μg/g (1.08 - 3.5 μg/g), while for Ag maximum concentration was observed in (10-B) and minimum in (11-A) 2.041 ± 0.024 μg/g (1.49 - 14.13 μg/g). The highest concentration in (11-B) and lowest in (10-B) for As; 1371.439 ± 0.085 μg/g (97 - 5694 μg/g). For Ba, the highest concentration was noticed in (6-B) and lower concentration in (4-C) with an average 0.134 ± 0.008 μg/g (0.66 - 0.86 μg/g). In (4-C) maximum concentration was noticed whereas minimum in (11-A) for Cd; with an average of 4.242 ± 0.02 μg/g (1.42 - 17.72 μg/g). Highest concentration in (11-B) and the lower concen-

tration in (8-A) for Co with an average of 3.934 ± 0.03 μg/g (1.07 - 19.09 μg/g), whereas for Cu highest concentration was noticed in (1-A) and lowest in (10-B) with average of 19.712 ± 0.012 μg/g (10 - 140 μg/g). For Mn, the highest concentration was noticed in (3-B) while the lowest in (11-A) with an average of 20.196 ± 0.056 μg/g (9.42 - 108.02 μg/g). For Ni, the highest concentration was observed in (3-B) and lowest in (2-A) with an average of 0.725 ± 0.012 μg/g (3.15 - 8.22 μg/g). The highest concentration in (8-A) and lowest in (10-B) for Pb; 858.666 ± 0.083 μg/g (498 - 1238 μg/g) with the highest concentration for Zn in (1-A) and the lowest in (3-A) sample. In all samples, chromium was not detected.

The total concentrations of metals in different samples ranged from 1.20 × 10³ to 6.08 × 10⁴ μg/g (Table 4). Higher total metal concentrations were found in dark-colored samples and lower concentrations were found in light-colored samples, with the exception of samples 6, 9, and 11. Table 4 demonstrates that lipstick products assigned to the high class category have the lowest average total metal concentrations (11-B), ranging from 3693.21 up to (10-B) 9361.91 μg/g, while the middle class has (8-A) 3475.1 to (8-B) 3068.91 μg/g, and the highest average total metal concentrations are found in lower class lipsticks, from (4-C) 1201.35 up to (2B) 60800.36 μg/g. This finding indicates that the higher class of lipsticks (more expensive, higher quality) is safer than the lower class (less expensive, lower quality), and is consistent with earlier research for lead and cadmium levels in various cosmetic brands [3] . The highest concentrations of the various elements detected were found in sample number 9 of the lower class brands, while the lowest level was in sample 8 of higher class brands (Table 5). Based on the average levels of heavy metal (Table 5), the samples can be arranged in the decreasing order: Fe > Al > Ba > Zn > Ni > Mn > Ti > Co > Cu > As > Pb > Ag > Cd > Cr. Most products in this study were detected to contain high concentrations of heavy metals, particularly Fe, Al, Ba, and Zn, which showed a high degree of variation among the samples (Figure 1).

A comparison of the data of this study (Table 6) with the literature (Table 7) of other countries revealed that, the concentrations of heavy metals in the

^†Average ± Standard deviation (SD); nd = not determined.

cosmetic products are not reported for Al, Ti, Ag, As, Ba, and Mn in six studies. Al is added to lipsticks as a stabilizer to keep colors from running, and to lip glosses, lipsticks, and nail polishes as a colorant. TiO₂ is used as a whitening agent, for softening reds into pinks, and as an antioxidant, both of which uses are approved by the FDA; the SASO allows up to 25% TiO₂ in cosmetic products. In the present study, the levels of Ti (in the form of TiO₂) was ranged between 0.0026% and 0.312%.The highest concentration of Fe found (59,702 μg/g) was found to be higher than that reported for lipstick products [6] [10] [19] [20] . The high of Fe is most likely attributed to the established role of iron compounds as colorants in cosmetics. On the other hand, Ag and As were found as impurities. Barium is banned by the SASO, but it was detected at levels up to 5694 μg/g owing to its use as a colorant. Cadmium is banned in cosmetics because of its toxicity, to which children are particularly susceptible [17] , while the

highest concentration of this element (0.86 μg/g) was found to be lower than the maximum value reported (2.4 μg/g) in literature [6] . Maximum concentration of Co (17.72 μg/g) was found higher than that reported in any study of cosmetic products. No Cr was detected in any product, though the detection limit was 1.00 μg/g. Maximum value of Cu (19.09 μg/g) was found to be higher than that reported in the most recent study (10). Levels of manganese, which is used as a catalyst for pigments and is not banned, were found up to 140 μg/g. The maximum value for Ni (108.02 μg/g) was found to be higher than that reported in most previous studies [6] [10] . The highest concentration of Pb (8.22 μg/g) was found to be lower than the reported values [6] [9] [10] [18] . The maximum value of Zn (1238 μg/g), which as an oxide has properties similar to TiO₂, was found to be higher than any report in the literature. Heavy metals bind with proteins in cells, leading to cell death and multiple diseases [26] . These heavy metals in lipsticks are impurities, as reported [27] . The slow liberation of these metals into the body means that they may cause damage after accumulating over time in various organs [14] [28] . Other studies have also reported heavy metal concentrations in various cosmetic products [2] .

The levels of 14 heavy metals including Al and Ba in lipstick products imported from different countries and from the local market in Jeddah, Saudi Arabia are reported. Only three of the samples contained low levels of Pb, and 4 samples showed very low levels of Cd; 6 samples had low levels of As, while all samples contained Baat various levels. The latter metals were banned by the SASO, making all of the products that contain them contraband. High levels of Fe and Al were noticed in most lipstick products. The light-colored lipsticks appear to be safer than the dark-colored ones in terms of metal concentrations. Continued use of such cosmetics products containing heavy metals may result in the slow liberation of these metals into the human body, which in turn causes harmful effects. Based on these findings, extensive uses of such products should be avoided. Thus, there is need for further assessment of risk to human health from exposure to cosmetics that are contaminated with heavy metals. Careful selection of the raw materials used in producing them with regard to heavy metal content can improve the safety of cosmetics and their impact on the environment.

Zainy, F.M.A. (2017) Heavy Metals in Lipstick Products Marketed in Saudi Arabia. Journal of Cosmetics, Dermatological Sciences and Applications, 7, 336-348. https://doi.org/10.4236/jcdsa.2017.74030