In this study, large seedlings of the same batch of fertilized P. Aibuhitensis eggs were hatched at a salinity level of 21 according to the Specifications for Artificial Propagation of Perinereis aibuhitensis (Grube) (DB32/T2450-2013). The clamworms were dug from the soil carefully and cultivated in clean seawater with a salinity level of 21 to empty the intestine. About 2700 healthy individuals with an initial average mass of 20.4 ± 0.3 mg were selected and divided into nine groups (each group in triplicate). The salinity levels of the groups were adjusted (via three increments per day) to 9, 12, 15, 18, 21, 24, 27, 30, and 33, respectively.

First, soil collected from a tidal flat in Sheyang county, Yancheng city, Jiangsu province in China, was washed with tap water repeatedly to remove salt, organic matter, and debris. Then, the soil was dried and sifted through a 10-mesh sieve and baked at 60˚C for 8 h. The soil was placed in polyethylene tanks (dimensions, 61 cm ´ 41 cm ´ 25 cm and sand depth, 10 cm) and dipped in clean seawater with salinity levels of 9, 12, 15, 18, 21, 24, 27, 30, and 33. After the soil was soaked completely, the seawater was removed, and the water-saturated soil was obtained.

The P. aibuhitensis specimens were cultivated in the breeding tank at the corresponding salinity and fed at 08:00 and 19:00 every day; the daily feeding dose was 1% - 2% and 2% - 3% fresh weight of the clamworms, respectively. Table 1 indicates the formula and nutrients in the feed. After feeding, a suitable amount of fresh water was sprayed into the tank to supply moisture and soften the pellet fodder. The remaining bait was cleaned after feeding for 1.5 - 2 h. During the rearing, the photoperiod in the room was 14 hours:10 hours (light: dark), and

Note: ¹Premix supplied the following minerals (g/kg) and vitamins (IU or mg/kg): 13.5 g CuSO₄・5H₂O, 58 g FeSO₄・7H₂O, 65 g ZnSO₄・7H₂O, 16 g MnSO₄・4H₂O, 10.8 g Na₂SeO₃・5H₂O, 12.64 g KI, 21.5 g CoCl₂・6H₂O, 1,600,000 IU VA, 500,000 IU VD₃, 9000 mg VE, 450 mg VK₃, 700 mg VB₁, 2500 mg VB₂, 1000 mg VB₆, 3.2 mg VB₁₂, 18,000 mg VC, 2000 mg pantothenate, 650 mg folic acid, 1500 mg niacin, 400 mg biotin, 300 mg myoinositol; ²Calculated by difference (dry matter - crude protein - crude lipid - ash) (Pedroetal., 2013); ³Computed as 21.3, 17.2, and 39.5 kJ/g of proteins, carbohydrates, and lipids, respectively (Cuzonetal., 1997).

soil temperature in the breeding tank varied from 23˚C to 26˚C. All the feeding experiments were conducted for 60 days at the Intertidal Organism Culture Laboratory in Yancheng Institute of Technology.

The original data were edited by Excel 2007 and then analyzed using single-factor analysis of variance (one-way ANOVA; SPSS 17.0 software). If the difference was significant, Duncan’s multiple comparison test was performed. Correlativity between the specific growth rate, feed conversion ratio, protein efficiency, and salinity levels were matched using the quadratic polynomial method. Data are presented as mean ± standard deviation (n = 3) values. P < 0.05 was used to assess the statistical significance of the results.

Data in Table 2 indicate that the level of salinity has a certain influence on the

Note: Means represent mean ± SE, n = 3. Means in the same line with different superscripts are significantly different (P < 0.05). Terminal average weight, TAW; survival rate, SR; specific growth ratio, SGR; feed conversion ratio, FCR, protein efficiency ratio, PER; each Perinereis aibuhitensis, EP.

growth indexes of clamworms. The survival rate of clamworms at a salinity level of 9 was significantly lower than that of the other groups (P < 0.05). However, the survival rate of P. aibuhitensis at the other salinity levels was not significant (P > 0.05). As the salinity level increased, the specific growth rate and protein efficiency of P. aibuhitensis showed a downward trend after the first increase, while the changing trend of the feed conversion ratio was just the opposite. At higher salinity levels from 27 to 33, the specific growth rate, feed conversion ratio, and protein efficiency of P. aibuhitensis were not significant (P > 0.05).

The efficiency relationship between salinity and specific growth rate, feed conversion ratio, and protein efficiency ratio was analyzed using the quadratic polynomial regression model. The regression equation for the relationship between specific growth rate and salinity is SGR = −0.00319S² + 0.16526S + 2.9228 (see Figure 1). When S = 25.9, specific growth rate was the highest. The regression equation for the relationship between feed conversion ratio and salinity is FCR = 0.00277S² − 0.14047S + 0.7757 (see Figure 2). When S = 25.36, feed conversion ratio was the lowest. The regression equation for the relationship between protein efficiency ratio and salinity is PER = −0.00463S² + 0.23665S + 0.01339 (see Figure 3). When S = 25.56, protein efficiency ratio was the highest. Thus, the optimal salinity range for P. aibuhitensis growth was 25.36 - 25.9.

Table 3 shows the different body compositions (moisture, crude protein, crude fat, and ash) of P. aibuhitensis due to different salinity levels. Overall, the body moisture content decreased with increasing salinity levels, but the difference in the salinity range of 15 to 33 was not significant (P > 0.05). Salinity had a significant influence on the crude protein content (P < 0.05), while it had no

Note: Means represent mean ± SE, n = 3. Means in the same line with different superscripts are significantly different (P < 0.05).

significant influence on crude body fat. Crude protein showed an increasing trend with the salinity increasing from 9 to 24, and decreased with the salinity increasing from 24 to 33. The ash content was relatively low when the salinity level was low. As the salinity level increased, the ash content increased gradually. However, in the salinity range of 18 to 33, the difference in ash content was not significant (P > 0.05).

Table 4 shows the main antioxidant enzyme levels (SOD and CAT) and amounts of two non-enzymatic antioxidant small molecules (GSH and MDA) under different salinity levels. The MDA content was significantly higher in the clamworms under low salinity levels of 9 to 18 than in those under salinity levels of 21 to 33 (P < 0.05). However, the MDA content was generally low and showed no obvious differences (P > 0.05) in the salinity range of 21 to 33. SOD and CAT activities showed a declining and rising trend when the salinity level increased gradually; the lowest SOD value was observed at the salinity level of 24, and the lowest CAT value, at 21.

Table 5 shows the total nitrogen content in the soil in which the P. aibuhitensis specimens were reared for 60 days under different salinity levels. Compared to the original value of TNC (0.215 ± 0.009 g/kg), the total nitrogen content of each group was obviously increased. Overall, the total nitrogen content first declined and then increased, and the lowest value was observed at the salinity level of 24. The total nitrogen content showed no significant differences (P > 0.05) in the salinity range of 21 to 27.

Note: Means represent mean ± SE, n = 3. Means in the same line with different superscripts are significantly different (P < 0.05).

Note: Means represent mean ± SE, n = 3. Means in the same line with different superscripts are significantly different (P < 0.05).

The survival rate of P. aibuhitensis at the salinity level of 9 was significantly lower than that of P. aibuhitensis in the other groups (P < 0.05); this is similar to the results obtained by Zhu et al. [21] . The main reason may be that the low-salinity environment exceeded the ability of P. aibuhitensis to regulate the osmotic pressure, resulting in the instability of the internal environment of the clamworms and metabolic disorders. Thus, some clamworms with a poor ability to adjust the osmotic pressure began to appear abnormal, and even died. Only clamworms with a strong adjustment ability can gradually adapt to a low-salinity environment. The growth performance indexes SGR and PER were significantly lower at low salinity levels of 9 to 12 than at high salinity levels, while FCR was significantly higher at low salinity levels of 9 to 12 than at high salinity levels (P < 0.05). The main reason is that low salinity has an adverse effect on feed conversion, oxygen consumption rate, metabolism, digestive enzyme activities, and immunity of aquatic animals [22] [23] . At the same time, osmoregulation and other related physiological functions need additional energy, resulting in low SGR and PER and high FCR and finally causing a decline in the growth performance [24] .

The quadratic polynomial regression model was used to analyze the efficiency relationship between salinity and specific growth rate, feed conversion ratio, and protein efficiency ratio of P. aibuhitensis, and the appropriate salinity range for the growth performance of P. aibuhitensis was observed to vary from 25.36 to 25.9. Within this range of salinity, the clamworms showed the highest specific growth rate and protein efficiency, lowest feed coefficient, and relatively high growth indicators. According to the principle of isotonic adjustment, aquatic animals such as euryhaline fish show the lowest penetration pressure, lowest metabolic rate, and highest rate of growth at the isotonic point [25] . When the salinity level is varied from 25.36 to 25.9, environmental salinity may be closer to the isotonic point of the clamworms, and they show a good growth trend. If the clamworms are cultivated at a lower or higher salinity environment in which the salinity level deviates greatly from the isotonic point, the clamworms have to use a lot of energy to regulate the osmotic pressure and maintain a stable internal environment; thus, the energy used for metabolism, growth, and other life activities is reduced [26] . Thus, low or high salinity will inhibit the growth of P. aibuhitensis. However, P. aibuhitensis showed better growth at high salinity than at low salinity.

The body moisture content of P. aibuhitensis decreased with increasing salinity level and was significantly higher at low salinity levels than at high salinity levels (P < 0.05), which is consistent with the Penaeus vannamei [27] . The main reason is that clamworms in a low-salt environment need to absorb more water to maintain homeostasis and enhance their ability to adapt to the environment.

Maximum crude protein contents were observed at the salinity level of 24. The energy clamworms use to perform osmotic regulation is mainly provided by the body lipids and proteins, so salinity can also affect the behavior, feeding, and immunity of clamworms [26] . Thus, the combined effects of salinity on the physiological functions of clamworms determine changes in their body fat and protein contents [24] . When the salinity level is around 24, body fluid of P. aibuhitensis may be very close to the water osmotic pressure, which means that less the energy required for regulating osmotic pressure, less the consumption of proteins and fat. As a result, the body fat and crude protein contents are higher. At higher salinity levels of about 24 to 33, the body ash content was at a high level, and the difference was not significant (P > 0.05).

An excess of reactive oxygen species (ROS), such as hydrogen peroxide (H₂O₂), not only kill invasion pathogens but also damage the body itself, such as converting unsaturated fatty acids, destroying the cell membrane structure, and causing DNA degradation [28] . MDA is a hazardous peroxide produced by peroxidation, and its content indicates the degree of oxidation. Antioxidant enzyme systems of aquatic animals (mainly SOD and CAT) play an important role in removing excess free radicals and alleviating body damage [29] . Thus, the antioxidant enzyme system is an important immune response system for aquatic animals to deal with salinity and other environmental stresses [30] .

Peroxidation can not only lead to elevated levels of MDA but also induce increased SOD and CAT activities and GSH content to clear the free radicals produced by peroxidation. Therefore, the level of antioxidant enzyme activity can reflect the physiological condition of P. aibuhitensis at different salinity levels to some extent, and it can be used as an important physiological indicator for measuring whether aquatic animals are stressed by salinity [31] . In this study, the P. aibuhitensis specimens were found to have high MDA content and SOD and CAT activities at low salinity. The main reason for this phenomenon may be because P. aibuhitensis experiences body stress, resulting in an increased metabolic rate, oxygen consumption rate, and demand for energy. The short-term increase in the contents of MDA and other antioxidant small molecules induces an increase in antioxidant enzyme activity, which helps to increase the resistance of P. aibuhitensis to the external environment; this was confirmed by a study on Takifugu obscurus conducted by Bian et al. [32] . In the salinity range of 21 to 33, MDA level in P. aibuhitensis is low and shows no differences with changes in salinity (P > 0.05). Considering the abovementioned growth indicators of P. aibuhitensis, it might be that in an appropriate salinity range, P. aibuhitensis shows a good growth trend and low active oxygen content. The antioxidant enzyme system can effectively remove free radicals and ROS from the body, meaning that free radical production and clearance are in dynamic equilibrium [29] .

Total nitrogen in the substrate refers to the sum of organic and inorganic nitrogen, such as NO 3 − , NO 2 − , NH 4 + , and NH₃, in the substrate. In this experiment, total nitrogen content of the sediment in which the clamworms were reared for 60 days was much higher than the soil total nitrogen.

At salinity in the range of 9 to 33, the total nitrogen content of the sediment decreased first as the salinity increased and then reached a minimum value when the salinity was around 24. Finally, it increased with increasing salinity levels. The following two points were speculated after the experiment, but further studies are required to prove them. 1) The total nitrogen content may be affected by the ammonia excretion rate of the clamworms. A previous study showed that salinity has an effect on the ammonia excretion rate [33] . In this experiment, the total nitrogen content increased at first and then decreased with an increase of salinity, and the lowest values were observed at the salinity level of 24. Salinity of about 24 may be more appropriate for the growth and survival of P. aibuhitensis with less direct elimination of ammonia produced by protein metabolism, resulting in the low ammonia excretion rate. Therefore, the total nitrogen content in the substrate was low. 2) The total nitrogen content of the substrate may be related to the digestive enzyme activities of the clamworms. Digestion and absorption of nutrition require the digestive enzymes of aquatic animals. Since the amount of ions may change when they are accumulated in the digestive glands of aquatic animals under different salinity conditions, the activities of digestive enzymes may be different; other mechanisms may also exist, for example, a change in salinity at a certain range may affect the endocrine physiology of aquatic animals, thereby affecting the secretion of digestive enzymes and dynamic changes [34] . This can be divided into activating effect, no effect, and inhibition [35] . P. aibuhitensis showed relatively better growth performance at the salinity of about 24. With the activating effect of salinity on the enzymatic activity of clamworms, the activities of the digestive enzymes, such as proteases, are increased, which means the higher apparent digestibility of clamworms, and lower organic nitrogen content in the feces.