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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article">
  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">OJF</journal-id>
      <journal-title-group>
        <journal-title>Open Journal of Forestry</journal-title>
      </journal-title-group>
      <issn pub-type="epub">2163-0429</issn>
      <publisher>
        <publisher-name>Scientific Research Publishing</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.4236/ojf.2017.74022</article-id>
      <article-id pub-id-type="publisher-id">OJF-78868</article-id>
      <article-categories>
        <subj-group subj-group-type="heading">
          <subject>Articles</subject>
        </subj-group>
        <subj-group subj-group-type="Discipline-v2">
          <subject>Earth&amp;Environmental Sciences</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>


          Effects of Sawdust, Forest Soil and Cow Dung Mixtures on Growth Characteristics of Blue Gum (&lt;i&gt;Eucalyptus saligna&lt;/i&gt;) Seedlings in South Kinangop Forest, Nyandarua, Kenya

        </article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
          <name name-style="western">
            <surname>Fredrick</surname>
            <given-names>Atanas Ashiono</given-names>
          </name>
          <xref ref-type="aff" rid="aff1">
            <sup>1</sup>
          </xref>
          <xref ref-type="corresp" rid="cor1">
            <sup>*</sup>
          </xref>
        </contrib>
        <contrib contrib-type="author" xlink:type="simple">
          <name name-style="western">
            <surname>Hellen</surname>
            <given-names>Kamiri Wangechi</given-names>
          </name>
          <xref ref-type="aff" rid="aff2">
            <sup>2</sup>
          </xref>
        </contrib>
        <contrib contrib-type="author" xlink:type="simple">
          <name name-style="western">
            <surname>Mwangi</surname>
            <given-names>James Kinyanjui</given-names>
          </name>
          <xref ref-type="aff" rid="aff3">
            <sup>3</sup>
          </xref>
        </contrib>
      </contrib-group>
      <aff id="aff2">
        <addr-line>School of Agriculture and Biotechnology, Department of Crop Science, Karatina University, Karatina, Kenya</addr-line>
      </aff>
      <aff id="aff1">
        <addr-line>Kenya Forest Service, South Kinangop Forest Station, Nairobi, Kenya</addr-line>
      </aff>
      <aff id="aff3">
        <addr-line>School of Natural Resources and Environmental Studies, Department of Natural Resources, Karatina University, Karatina, Kenya</addr-line>
      </aff>
      <author-notes>
        <corresp id="cor1">
          * E-mail:<email>ashionofa@yahoo.com(FAA)</email>;
        </corresp>
      </author-notes>
      <pub-date pub-type="epub">
        <day>31</day>
        <month>08</month>
        <year>2017</year>
      </pub-date>
      <volume>07</volume>
      <issue>04</issue>
      <fpage>373</fpage>
      <lpage>387</lpage>
      <history>
        <date date-type="received">
          <day>March</day>
          <month>30,</month>
          <year>2017</year>
        </date>
        <date date-type="rev-recd">
          <day>Accepted:</day>
          <month>August</month>
          <year>29,</year>
        </date>
        <date date-type="accepted">
          <day>September</day>
          <month>1,</month>
          <year>2017</year>
        </date>
      </history>
      <permissions>
        <copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement>
        <copyright-year>2014</copyright-year>
        <license>
          <license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p>
        </license>
      </permissions>
      <abstract>
        <p>


          Forestation programs, in Kenya have been hindered by, among others, high cost of tree seedling production and longtime seedlings stay in the nursery. This has been attributed to poor quality of forest soil and unavailability of alternative growth media. Study was carried out in South Kinangop forest station to evaluate the potential of mixing sawdust and cow dung with forest soil to form a nutrient rich growth media. Using forest soil; sawdust, cow dung and mixture of these components, this study sought to determine growth characteristics of Eucalyptus saligna seedlings under different conditions available in the nursery. The experiment was laid out in a Completely Randomized Design (CRD) with six treatments comprising of Saw dust alone (Tr 1), Forest soil alone (Tr 2); Sawdust: Cow dung mixture in the ratio of 1:1 (Tr 3); Forest soil to Sawdust-Cow dung mixture (1:1) in ratio of 1:1 by weight (Tr 4); Forest soil to Sawdust-Cow dung mixture (1:1) in ratio of 1:2 by weight (Tr 5) and Forest soil to Sawdust-Cow dung (1:1) in ratio of 1:3 by weight (Tr 6). Treatments were replicated three times and an experimental unit consisted of 110 seedlings of which 15 were tagged for height and root collar diameter measurement. The remaining 95 were used for biomass determination by destructive sampling. The results showed that Sawdust: Cow dung mixtures (Tr 3) had the highest measurements for height, root collar diameter and biomass at week 20 when compared to the rest of growth mixtures. Height measurements were significantly high (13.81 cm) for the Sawdust: Cow dung mixtures while the variation in root collar diameter and biomass were not significantly different among the treatments. Sawdust alone did not favor seedling growth and recorded the lowest measurements in height, root collar diameter and biomass. Nutrient release from the growth media during the experimental period was equally high for the Saw dust: Cow dung mixture (Tr 3) which was ideal media for raising tree seedlings. The findings of this study illustrate the usability of sawdust and cow dung in enhancing the productivity of tree nurseries and shortening the time taken for raising seedlings in the tree nursery. The findings also propose a safe disposal of saw dust that poses as an environmental waste.

        </p>
      </abstract>
      <kwd-group>
        <kwd>CowDung</kwd>
        <kwd> Forest Soil</kwd>
        <kwd> Sawdust</kwd>
        <kwd> Tree Seedling</kwd>
      </kwd-group>
    </article-meta>
  </front>
  <body>
    <sec id="s1">
      <title>1. Introduction</title>
      <p>Saw dust is one of the byproducts of saw milling, which is a major economic activity practiced in countries with large forests resources (  Leconte  et al., 2009</p>
      <p>Saw dust has also been used as a soil conditioner in general agriculture (  Garner, 2014 ). Saw dust is purely wood material comprising of 50% carbon (  Parry, 2007 ). Its incorporation into the soil enhances a buildup of soil organic carbon pool which is slow to decompose and which can act as agricultural strategy on climate change mitigation that reduces emission of greenhouse gases.</p>
      <p>Most of our soils are greatly in need of organic matter because the usual crop rotations and degradation seldom maintain soil humus at desirable levels and thus saw dust can provide these nutrients over a long period if decomposition is managed appropriately. Saw dust is also a largely sterile media that does not harbor microorganisms due to its low nutrient content (  Okalebo et al., 2002  )</p>
      <p>Decomposition is one of the most important processes that accounts for carbon and nutrient recycling on crop land and in the forest systems (Shi, 2013)  . It is controlled by factors such carbon to nitrogen ratio  (Richard &amp; Trautmann, 2014)  , pH and nutrient balance in the medium, particle size, temperature, moisture content and porosity  (Bernal et al., 2009)  . The slow rate of decomposition of saw dust and temporary depression of nitrogen has been the principal objection to its use in agricultural fields  (Garner, 2014) . In addition, sawdust is low in readily available plant nutrients when compared to livestock manure which is rich in microbes responsible for rapid decomposition of organic matter and pH of 6.0 - 9.0 which is ideal for microbial activity (Pennington, 2009).</p>
      <p>Livestock manure (cow dung) has low carbon to nitrogen ratio of 25 - 30 which is ideal for provision of required energy for microbial activity as well as a high water holding capacity. The high concentration of macro nutrients in cow dung provide initial nutrients required for the decomposition process. These factors make cow dung an ideal component for mixing with sawdust to enhance its decomposition and meet the immediate and long term plant nutrient requirements. Cow dung has been extensively used in crop production in Kenya and is often recommended as a soil nutrient replenishment strategy in low income countries (Okalebo et al., 2006). Forest soils are commonly used to raise seedlings in the nursery. The soils are collected from natural forests. For example in motanne forests in Kenya, forest soils are collected under Tombeya torrida. The soils may not contain all essential nutrients either due to leaching or utilization by under growth. Addition of small quantities of inorganic fertilizer is recommended as a supplement for good growth of seedlings in the nursery (Evans, 1983).</p>
      <p>Seedlings height is one of growth parameters used to determine planting stage of seedlings in the field. Lamprecht (1989) recommended a height of 10 - 25 cm as most ideal for planting out Eucalyptus saligna in the field for better survival. However, Evans (1983) showed that height is not satisfactory for grading plants and recommended root collar diameter which is highly correlated with survival of the seedlings in the field.</p>
      <p>There exists minimal knowledge on how sawdust can be utilized as a growth medium in forest seedlings establishment as well as the appropriate mixing ratios for enhanced decomposition. Thus, this study was aimed at mixing sawdust with cow dung and/or forest soil to form a nutrient rich growth media that can be used to raise quality and fast growing tree seedlings. Specifically, the study sought to find out how the growth media comprising sawdust, forest soil and/or cow dung affects growth characteristics of Blue gum (Eucalyptus saligna) seedlings raised in South Kinangop Forest Station of Kenya.</p>
    </sec>
    <sec id="s2">
      <title>2. Materials and Methods</title>
      </sec>
      <sec id="s2_1">
        <title>2.1. Study Area</title>
        <p>
          The study was carried out in South Kinangop forest station in the Aberdare Forest Reserve (<xref ref-type="fig" rid="fig1">Figure 1</xref>). The station is located on latitude S 00˚43'28.4&quot;, longitude E036˚40'51.7&quot; and 2545 m elevation. The forest occupies a total 6660 hectares and forms part of the Aberdare range which is one of the major water towers serving Lake Naivasha to the north and Tana River to the south. The climate at South Kinangop is modified equatorial climate with average temperature of 12.3˚C and annual rainfall of about 1590 mm distributed bi-modally with peaks in late April and late November.
        </p>
      </sec>
      <sec id="s2_2">
        <title>2.2. Research Design, Experimental Layout and Data Collection</title>
        <p>The field layout comprised of six treatments (Tr 1 - 6) in a completely randomized design with three (3) replications.</p>
        <fig id="fig1"  position="float">
          <label>
            <xref ref-type="fig" rid="fig1">Figure 1</xref>
          </label>
          <caption>
            <title> Map of the study area showing the south Kinangop</title>
          </caption>
          <graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/1-1620433x2.png"/>
        </fig>
      </sec>
      <sec id="s2_3">
        <title>2.3. Growth Media Preparation</title>
        <p>Forest soil was dug and collected from the natural forest stands of Dombeya torrida within South Kinangop forest. Fine sawdust mainly from pine (Pinus patula) trees was collected from nearby sawmill while cow dung was sourced from homesteads near the forest margins. The sawdust, forest soil and cow dung were heaped separately in the nursery and preparations for the growth media carried out during the dry period (August 2015). The treatment mixtures were allowed to decompose for three months under shade so as to improve on aeration, reduce instances of dumping off disease to seedlings, reduce competition of nutrients from weeds, and increase water retention capacity.</p>
      </sec>
      <sec id="s2_4">
        <title>2.4. Tree Seedling Establishment and Management</title>
        <p>
          Eucalyptus saligna seeds were acquired from Kenya Forest Research Institute (KEFRI), Muguga Seed Centre and sown in a seed bed of sand, two weeks before the completion of decomposition period of the nursery growth media. After germination the seedlings were allowed to grow for 14 days in the seed bed and on the 15<sup>th</sup> day after germination, the seedlings were pricked out into polythene tubes at one seedling per tube. In total three hundred and thirty (330) polythene tubes of size 3’’ by 4’’ were filled with the growth media for each of the treatment described in <xref ref-type="table" rid="table1">Table 1</xref>. A total of 1980 tubes were filled for the six treatments. The tubes were arranged in an experimental unit of 110 seedlings per replicate which were then completely randomized and replicated three times. The seedlings were allowed to grow for 6 months under open and uncontrolled climatic conditions. During the experimental period, temperatures ranged from 19.9˚C during the day to 3.2˚C at night with mean temperature being 12.3˚C. Humidity ranged from 100% during early hours of the day to 40% late in the afternoon and an average of 65%. Sunlight was controlled through shading after pricking out from week 1 up to week 2. Afterward the shade was removed to allow for total exposure of seedlings to sunlight.
        </p>
      </sec>
      <sec id="s2_5">
        <title>2.5. Data Collection (Measurement of Height, Root Collar Diameter, Biomass Assessments and Nutrient Analysis)</title>
        <p>Data collection involving height, root collar diameter and biomass sampling was</p>
        <table-wrap id="table1" >
          <label>
            <xref ref-type="table" rid="table1">Table 1</xref>
          </label>
          <caption>
            <title> Treatment description showing the growth media mixtures used in the study</title>
          </caption>
          </table-wrap>
        </sec>
          </body>
          <back>
            <ref-list>
              <title>References</title>
              <ref id="scirp.78868-ref1">
                <label>1</label>
                <mixed-citation publication-type="other" xlink:type="simple">Becker, M., Ladha, J. K., &amp; Ottow, J. C. G. (1994). Nitrogen Losses and Lowland Rice Yield as Affected by Residue Nitrogen Release. Soil Science Society of America, 58, 1660-1665. https://doi.org/10.2136/sssaj1994.03615995005800060012x</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref2">
                <label>2</label>
                <mixed-citation publication-type="other" xlink:type="simple">Bernal, M. P., Alburquerque, J. A., &amp; Moral, R. (2009). Composting of Animal Manures and Chemical Criteria for Compost Maturity Assessment. A Review. Bioresource Technology, 100, 5444-5453. https://doi.org/10.1016/j.biortech.2008.11.027</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref3">
                <label>3</label>
                <mixed-citation publication-type="other" xlink:type="simple">Brady, N. C., &amp; Weil, R. R. (2002). The Nature and Property of Soils (13th ed.). London: Pearson Education.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref4">
                <label>4</label>
                <mixed-citation publication-type="other" xlink:type="simple">Cassman, K. G., Dobermann, A., Cruz, P. C. S., Gines, G. C., Samson, M. I., Descalsota, J. P., Alcantara, J. M., Dizon, M. A., &amp; Olk, D. C. (1996). Soil Organic Matter and the Indigenous Nitrogen Supply of Intensive Irrigated Rice Systems in the Tropics. Plant and Soil, 182, 267-278. https://doi.org/10.1007/BF00029058</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref5">
                <label>5</label>
                <mixed-citation publication-type="other" xlink:type="simple">Chardust Ltd. and Spectrum Technical Services (2004). The Use of Biomass Wastes to Fabricate Charcoal Substitutes in Kenya. Nairobi: Chardust.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref6">
                <label>6</label>
                <mixed-citation publication-type="other" xlink:type="simple">Evans, J. (1983). Plantation Forestry (3rd ed.). Oxford: Oxford University Press.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref7">
                <label>7</label>
                <mixed-citation publication-type="book" xlink:type="simple">Garner, E (2014). Sawdust as a Mulch and Soil Amendment for Rhododendrons and Azaleas. A. N. Roberts, &amp; A. R. S. Bulletin (Eds.), Journal American Rhododendron Society (e-Journals), 5, 58.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref8">
                <label>8</label>
                <mixed-citation publication-type="journal" xlink:type="simple">
                  <name name-style="western">
                    <surname>Kamiri</surname>
                    <given-names> H.</given-names>
                  </name>,<name name-style="western">
                    <surname> Kreye</surname>
                    <given-names> C.</given-names>
                  </name>,<name name-style="western">
                    <surname> &amp; Becker</surname>
                    <given-names> M. </given-names>
                  </name>,<etal>et al</etal>. (<year>2013</year>)<article-title>. Dynamics of Agricultural Use Differentially Affect Soil Properties and Crop Response in East African Wetlands</article-title><source> Wetlands Ecology and Management</source><volume> 21</volume>,<fpage> 417</fpage>-<lpage>431</lpage>.<pub-id pub-id-type="doi"></pub-id>
                </mixed-citation>
              </ref>
              <ref id="scirp.78868-ref9">
                <label>9</label>
                <mixed-citation publication-type="other" xlink:type="simple">Lamprecht, H. (1989). Silviculture in the Tropics: Tropical Forest Ecosystems and Their Tree Species-Possibilities and Methods for Their Long-Term Utilization. Frankfurt: Federal Republic of Germany.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref10">
                <label>10</label>
                <mixed-citation publication-type="other" xlink:type="simple">Leconte, M. C., Mazzarino, M, J., Satti, P., Lglesias, M. C., &amp; Laos, F. (2009). Co-Composting Rice Hull and/or Sawdust with Poultry Manure in NE Argentina. Waste Management, 29, 2446-2453. https://doi.org/10.1016/j.wasman.2009.04.006</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref11">
                <label>11</label>
                <mixed-citation publication-type="other" xlink:type="simple">MENR, Forest Department Technical Orders (1996). Treatment of Eucalyptus Plantations. Nairobi: MENR.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref12">
                <label>12</label>
                <mixed-citation publication-type="other" xlink:type="simple">Mexal, J. G., &amp; Landis, L. D. (1990). “Target Seedling Concepts: Height and Diameter.” Target Seedling Symposium, Meeting of the Western Forest Nursery Associations, General Technical Report RM-200. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Forest and Range Experiment Station.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref13">
                <label>13</label>
                <mixed-citation publication-type="journal" xlink:type="simple">
                  <name name-style="western">
                    <surname>Myrold</surname>
                    <given-names> D. D. </given-names>
                  </name>,<etal>et al</etal>. (<year>1998</year>)<article-title>. Transformations of Nitrogen</article-title><source> Principles and Applications of Soil Microbiology</source><volume> 12</volume>,<fpage> 259</fpage>-<lpage>294</lpage>.<pub-id pub-id-type="doi"></pub-id>
                </mixed-citation>
              </ref>
              <ref id="scirp.78868-ref14">
                <label>14</label>
                <mixed-citation publication-type="other" xlink:type="simple">Okalebo, J. R., Gathua, K. W., &amp; Woomer, P. L. (2002). Laboratory Methods of Soil and Plants Analysis: A Working Manual (2nd ed.). Nairobi: SACRET Africa.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref15">
                <label>15</label>
                <mixed-citation publication-type="other" xlink:type="simple">Okalebo, J. R., Othieno, C. O., Woomer, P. L., Karanja, N. K., Semoka, J. R., Bekunda, M. A., &amp; Mukhwana, E. J. (2006). Available Technologies to Replenish Soil Fertility in East Africa. Nutrient Cycling in Agroecosystems, 76, 153-170. https://doi.org/10.1007/s10705-005-7126-7</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref16">
                <label>16</label>
                <mixed-citation publication-type="journal" xlink:type="simple">
                  <name name-style="western">
                    <surname>Onchieku</surname>
                    <given-names> J. M.</given-names>
                  </name>,<name name-style="western">
                    <surname> &amp; Chikamai</surname>
                    <given-names> B. N. </given-names>
                  </name>,<etal>et al</etal>. (<year>2013</year>)<article-title>. Development of Mathematical Models for Estimation of the Quantity of Biomass Residues</article-title><source> International Journal of Applied Science and Technology</source><volume> 3</volume>,<fpage> 65</fpage>-<lpage>76</lpage>.<pub-id pub-id-type="doi"></pub-id>
                </mixed-citation>
              </ref>
              <ref id="scirp.78868-ref17">
                <label>17</label>
                <mixed-citation publication-type="journal" xlink:type="simple">
                  <name name-style="western">
                    <surname>Ossom</surname>
                    <given-names> E. M.</given-names>
                  </name>,<name name-style="western">
                    <surname> &amp; Kunene</surname>
                    <given-names> S. S. </given-names>
                  </name>,<etal>et al</etal>. (<year>2010</year>)<article-title>. Influence of Growing Media on Stem Diameter Andecological Characteristics of Pinuspatula Seedlings in Swaziland</article-title><source> World Journal of Agricultural Sciences</source><volume> 6</volume>,<fpage> 652</fpage>-<lpage>659</lpage>.<pub-id pub-id-type="doi"></pub-id>
                </mixed-citation>
              </ref>
              <ref id="scirp.78868-ref18">
                <label>18</label>
                <mixed-citation publication-type="book" xlink:type="simple">Parry, M. L. (Ed.) (2007). Climate Change 2007—Impacts, Adaptation and Vulnerability: Working Group II Contribution to the Fourth Assessment Report of the IPCC (Vol. 4). Cambridge: Cambridge University Press.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref19">
                <label>19</label>
                <mixed-citation publication-type="other" xlink:type="simple">Pennington, J. A., VanDevender, K., &amp; Jennings, J. A. (2009). Nutrient and Fertilizer Value of Dairy Manure. Fayetteville: University of Arkansas.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref20">
                <label>20</label>
                <mixed-citation publication-type="other" xlink:type="simple">Richard, T., &amp; Trautmann, N. (2014). How to Calculate C/N Ratio for Three Materials.</mixed-citation>
              </ref>
              <ref id="scirp.78868-ref21">
                <label>21</label>
                <mixed-citation publication-type="other" xlink:type="simple">Shi, J. M. M. (2013). Decomposition Rates and Nutrients Release of Different Cover Crops in Organic Farms System. Lincoln, NE: University of Nebraska-Lincoln.</mixed-citation>
              </ref>
            </ref-list>
          </back>
</article>