In order to improve the concentration of selenium in the maifanite concentrate, the optimum experimental conditions were obtained by orthogonal experimentas follows: size of maifanite was 10 mesh, concentration of maifanite was 15 (expressed as the quality of maifanite divided by that of water), soaking time was 60 min at room temperature, heating temperature was 70 oC, heating time was 20 min, pH value was 6. Under this condition, the concentrations of each element (ppm)determined by ICP-AES were: Fe 0.099, Cu 0.035, Mn 0.051, Zn 0.019, Se 0.028, Al 0.000.a ven in this document.
Metal dyshomeostasis was one of pathological characterizations of Alzheimer’s disease (AD) [
Many mineral elements were produced after the primary maifanite mineral was immersed into water [
The maifanite was purchased from the Guifeng Maifanite Company in Guangxi Guiping City, China. Deionized water (18 MΩ cm, Milli-Q gradient system, Millipore) was used throughout the experiments.
By the inductively coupled plasma-atomic emission spectroscopy (ICP-AES; IRIS Intrepid II XSP, USA Thermo Elemental), the elements Fe, Cu, Zn, Mn, Se, and Al were analyzed in the optimum dissolution conditions of maifanite. The measurement conditions were 1200 W from the radio-frequency generator, a plasma argon flow rate of 15 L/min, a cooling gas flow of 14 L/min, a carrier gas flow of 1.0 L/min, a 20-μm entrance slit, a 30-μm exit slit, a height of observation of 15mm, and an integration time lapse of 5s.
Software of orthogonal designing assistant II V3.1 was used for the evaluation of the statistical experimental design. A five-level and six-factor orthogonal experiment (
According to the orthogonal experiment design (L25-5-6 table), twenty-five experiments should be accomplished. As shown in
| No. | 1 | 2 | 3 | 4 | 5 | 6 |
|---|---|---|---|---|---|---|
| Factor name | Size/mesh | Concentration1) | soaking time/min2) | heating temperature/˚C | heating time/min | pH value |
| level 1 | 10 | 1 | 10 | 50 | 20 | 5.00 |
| level 2 | 50 | 5 | 30 | 60 | 40 | 6.00 |
| level 3 | 100 | 10 | 60 | 70 | 60 | 7.00 |
| level 4 | 150 | 15 | 90 | 80 | 80 | 8.00 |
| level 5 | 200 | 25 | 120 | 90 | 120 | 9.00 |
1)expressed as the quality of maifanite divided by that of water. 2)soaking time at room temperature.
| column | 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|---|
| Factor name | Size/mesh | Concentration1) | soaking time/min2) | heating temperature/˚C | heating time/min | pH value | Results/ppm |
| experiment 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0.035 |
| experiment 2 | 1 | 2 | 2 | 2 | 2 | 2 | 0.022 |
| experiment 3 | 1 | 3 | 3 | 3 | 3 | 3 | 0.038 |
| experiment 4 | 1 | 4 | 4 | 4 | 4 | 4 | 0.036 |
| experiment 5 | 1 | 5 | 5 | 5 | 5 | 5 | 0.014 |
| experiment 6 | 2 | 1 | 2 | 3 | 4 | 5 | 0.031 |
| experiment 7 | 2 | 2 | 3 | 4 | 5 | 1 | 0.023 |
| experiment 8 | 2 | 3 | 4 | 5 | 1 | 2 | 0.026 |
| experiment 9 | 2 | 4 | 5 | 1 | 2 | 3 | 0.027 |
| experiment 10 | 2 | 5 | 1 | 2 | 3 | 4 | 0.025 |
| experiment 11 | 3 | 1 | 3 | 5 | 2 | 4 | 0.022 |
| experiment 12 | 3 | 2 | 4 | 1 | 3 | 5 | 0.019 |
| experiment 13 | 3 | 3 | 5 | 2 | 4 | 1 | 0.021 |
| experiment 14 | 3 | 4 | 1 | 3 | 5 | 2 | 0.019 |
| experiment 15 | 3 | 5 | 2 | 4 | 1 | 3 | 0.015 |
| experiment 16 | 4 | 1 | 4 | 2 | 5 | 3 | 0.027 |
| experiment 17 | 4 | 2 | 5 | 3 | 1 | 4 | 0.024 |
| experiment 18 | 4 | 3 | 1 | 4 | 2 | 5 | 0.022 |
| experiment 19 | 4 | 4 | 2 | 5 | 3 | 1 | 0.029 |
| experiment 20 | 4 | 5 | 3 | 1 | 4 | 3 | 0.027 |
| experiment 21 | 5 | 1 | 5 | 4 | 3 | 2 | 0.023 |
| experiment 22 | 5 | 2 | 1 | 5 | 4 | 3 | 0.026 |
| experiment 23 | 5 | 3 | 2 | 1 | 5 | 4 | 0.017 |
| experiment 24 | 5 | 4 | 3 | 2 | 1 | 5 | 0.038 |
| experiment 25 | 5 | 5 | 4 | 3 | 2 | 1 | 0.029 |
| mean value 1 | 0.029 | 0.028 | 0.025 | 0.025 | 0.028 | 0.027 | |
| mean value 2 | 0.026 | 0.023 | 0.023 | 0.027 | 0.024 | 0.018 | |
| mean value 3 | 0.019 | 0.025 | 0.030 | 0.028 | 0.027 | 0.032 | |
| mean value 4 | 0.026 | 0.030 | 0.027 | 0.024 | 0.027 | 0.025 | |
| mean value 5 | 0.027 | 0.022 | 0.022 | 0.023 | 0.020 | 0.025 | |
| range | 0.010 | 0.008 | 0.008 | 0.005 | 0.008 | 0.014 |
1)expressed as the quality of maifanite divided by that of water. 2)soaking time at room temperature.
0.099, Cu 0.035, Mn 0.051, Zn 0.019, Se 0.028, Al 0.000.
According to the orthogonal experiment design, regarded Se content as the index of dissolution performance, the optimum dissolution conditions of maifanite were obtained as follows: size of maifanite was 10 mesh, concentration of maifanite was 15 (expressed as the quality of maifanite divided by that of water), soaking time was 60 min at room temperature, heating temperature was 70˚C, heating time was 20 min, pH value was 6. Under this condition, the concentrations of each element (ppm) determined by ICP-AES were: Fe 0.099, Cu 0.035, Mn 0.051, Zn 0.019, Se 0.028, Al 0.000 ure/K”.
This work was supported by the Sub Project of the Key Laboratory of Generic Technology Research and development of Traditional Chinese Drugs Pharmaceutics in Guangxi colleges and Universities (No. ZJGX201402005), which affiliated to Promotion Project of Science and Technology Innovation Ability in Guangxi Colleges and Universities (approved by the Education Department of Guangxi Government, China, No. [
Liao, H.L., Jiang, L.F., Wei, C.J., Huang, H., Pan, J. and Luo, C.T. (2017) Applied Orthogonal Experiment Design for the Optimum Extraction Conditions of High Concentration Selenium from Maifanite. Detection, 5, 1-4. https://doi.org/10.4236/detection.2017.51001