This study was conducted in march-september in 2015 in Science and technology innovation park in the south campus of Shandong agricultural university, Taian, China. The tested plants are one-year Malus hupehensis. var. pingyiensis seedlings potted in soil. The pot used in this test has 36 cm mouth diameter, 27 cm height, the soil weight of each pot is 10.2 kg. The soil has the following properties: soil available nitrogen 54.9 mg・kg⁻¹, available phosphorus 27.3 mg・kg⁻¹, available potassium 33.9 mg・kg⁻¹, organic matter 12.4 g・kg⁻¹, pH 7.3. The organic fertilizer has the following nutrients: soil available nitrogen 255.5 mg・kg⁻¹, available phosphorus 50.7 mg・kg⁻¹, available potassium 65.9 mg・kg⁻¹, organic matter 164.3 g・kg⁻¹. The tested lignin is alkaline lignin manufactured from the company of Shandong province.

Firstly, 0, 1.5, 2.5 g・kg⁻¹ lignin was added in the soil with organic fertilizer content 10% and 40%, respectively (Table 1), secondly, the above materials as experimental soil fully mixed with were put into the pots. The one-year Malus hupehensis. var. pingyiensis seedlings were planted in these pots, each treatment was designed in triplicate.

The roots and soil were picked up, and the sorted out roots were used to measure. The soil was sampled in September in 2015. After the surface soil was re-

moved, root activity, the seized soil through natural air dry was applied to determine soil nutrient content and soil enzyme activities.

Root activity was measured according to the triphenyltetrazolium chloride method [10] . Soil organic matter was measured according to the method [11] . The content on soil alkaline N, soil available P, soil available K was measured based on the method [12] .

The data in the article were analyzed through Excel statistical software and SPSS 17.0 statistical software package.

The root activity of Malus hupehensis. var. pingyiensis became larger with the increase of lignin concentration, regardless of 10% or 40% organic fertilizer content. The root activity of the species in 2.5 g・kg⁻¹ lignin treatment became bigger by 89.5% compared with that of the CK (Figure 1(a)). Under the condition of 40% organic fertilizer, the root activity of the species in 1.5 g・kg⁻¹ lignin treatment was no significant difference compared with that in the 2.5 g・kg⁻¹ lignin treatment, and that of the species in 2.5 g・kg⁻¹ lignin treatment was higher 55.0% than that in the CK2 (Figure 1(b)).

Soil nutrients differently varied in those lignin treatments (Figure 2). Soil available N and organic matter became gradually larger with the increase of lignin used. Among those treatments, that of soil available N and organic matter were largest in 2.5 g・kg⁻¹ lignin treatment, they raised by 27.3, 68.4% compared with that of CK1 under 10% organic fertilizer, respectively, and increased by 42.8%, 15.2% compared with that of CK2 under 40% organic fertilizer, respectively.

Under 10% organic fertilizer, the content of soil available P and organic matter of 1.5 g・kg⁻¹ lignin treatment became larger by 5.6%, 49.1%, respectively, soil alkaline N of 1.5 g・kg⁻¹ lignin treatment had an decrease compared with that of CK1. The 1.5 g・kg⁻¹ lignin treatment elevated the content soil available K by 16.0% compared with that of CK1.

Under 40% organic fertilizer, the two concentrations of lignin treatment elevated the content of soil available P, but had no significant difference compared with that of CK2. Different concentrations of lignin treatments decreased the content of rhizosphere soil available potassium, and that of rhizosphere soil available K in 2.5 g・kg⁻¹ lignin treatment lowered 8.5% compared with that of CK2 (Figure 2).

Soil fertility mainly depends on the amount of soil nutrients. The content of soil nutrients significantly raised after soil was applied with organic compound fertilizer. Soil organic matter correlated positively with soil organic carbon (SOC). Straw, lignin and biochar obviously increased the content of SOC [13] . Lignin could reduced the Fixation of soil P by metal ions, insoluble P could be rapidly resolved, which promoted the plant growth [14] . After used the lignin slow-release fertilizer in soil, the content of effective K raised, the K requirement for plant had an increase with its growth, which resulted in the steady declination of soil available K content [15] .

Under the application of organic fertilizer, lignin increased the content of alkaline N and organic matter. In the condition of 10% organic fertilizer, the amount of soil available P and K became larger, the result was coincided with the informed report [16] , the reason lied in that lignin enlarged the effectivity of soil P and N, promoted the accumulation of soil available K [15] . However, the content of soil alkaline N in 1.5 g・kg⁻¹ lignin treatment had an decrease, the reason might be that the thriving trees in 1.5 g・kg⁻¹ lignin treatment had assimilated much more soil alkaline N. In the condition of 40% organic fertilizer, the amount of soil available K became much smaller with the concentration of lignin among the treatments, which might be that lignin promoted the Malus hupehensis. var. pingyiensis growth, this tree absorbed more soil K, the study was similar to the reported research [16] . The amount of soil available P was lower in the 2.5 g・kg⁻¹ lignin treatment than that in the 1.5 g・kg⁻¹ lignin treatment, it might be that the trees of 2.5 g・kg⁻¹ lignin treatment were higher among the treatments and they consumed more soil P.

Root activity is an important index reflecting the ability of root to absorb soil nutrients and water and directly affecting the growth of plant. Root activity was affected by organic matter, fertilizer, plant cultivars, microbial number and other environmental factors [16] [17] . The application of lignin increased root activity of Malus hupehensis. var. pingyiensis, the reason may be that lignin improved the content of soil nutrients (Figure 2), promoted the absorption of root to soil nutrients.