<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">OALibJ</journal-id><journal-title-group><journal-title>Open Access Library Journal</journal-title></journal-title-group><issn pub-type="epub">2333-9705</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/oalib.1103467</article-id><article-id pub-id-type="publisher-id">OALibJ-75149</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biomedical&amp;Life Sciences</subject><subject> Business&amp;Economics</subject><subject> Chemistry&amp;Materials Science</subject><subject> Computer Science&amp;Communications</subject><subject> Earth&amp;Environmental Sciences</subject><subject> Engineering</subject><subject> Medicine&amp;Healthcare</subject><subject> Physics&amp;Mathematics</subject><subject> Social Sciences&amp;Humanities</subject></subj-group></article-categories><title-group><article-title>
 
 
  Pollen Evidence of a Humid Mid-Holocene Climate from Bahar Nuur, Inner Mongolia
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Changqing</surname><given-names>Huang</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Lanlan</surname><given-names>Guo</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib></contrib-group><aff id="aff1"><addr-line>Department of Geography and Tourism, Tongren University, Tongren, China</addr-line></aff><aff id="aff2"><addr-line>Key Laboratory of Environmental Change and Natural Disaster, Ministry of Education &amp;amp; State Key Laboratory of Earth Sur-face Processes and Resource Ecology, Beijing Normal University, Beijing, China</addr-line></aff><pub-date pub-type="epub"><day>02</day><month>03</month><year>2017</year></pub-date><volume>04</volume><issue>03</issue><fpage>1</fpage><lpage>16</lpage><history><date date-type="received"><day>February</day>	<month>17,</month>	<year>2017</year></date><date date-type="rev-recd"><day>Accepted:</day>	<month>March</month>	<year>28,</year>	</date><date date-type="accepted"><day>March</day>	<month>31,</month>	<year>2017</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
   
   A high-resolution pollen-based bioclimatic reconstruction from Bahar Nuur Lake in Inner Mongolia, China, supported by 15 AMS dates, is compared with other records in the Ordos Plateau and stalagmite record from Dongge cave in order to better understand the Holocene climate history in semi-arid East Asian monsoon margin area better. A humid climate prevailed in the Ordos Plateau during 6.3-4.4 ka BP, as indicated by 
   high broadleaf-tree pollen percentages and pollen concentrations. Due to a strong Asian monsoon, the mid-Holocene climatic optimum exhibited a delayed vegetative response on the Ordos Plateau. 
  
 
</p></abstract><kwd-group><kwd>Lake Sediment</kwd><kwd> Pollen Record</kwd><kwd> Climate Change</kwd><kwd> Ordos Plateau</kwd><kwd> Mid-Holocene</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>Holocene climate change has been a focus of global change studies because understanding the climatic patterns, processes and forcing mechanisms during this period can shed light upon future climate change and its associated regional eco- logical responses [<xref ref-type="bibr" rid="scirp.75149-ref1">1</xref>] . To this end, the reconstruction of the temporal patterns of change in climatically sensitive regions is of great importance [<xref ref-type="bibr" rid="scirp.75149-ref2">2</xref>] . The semi-arid East Asian monsoon margin area is one of the regions, most sensitive to large- scale climatic changes. However, our knowledge regarding the recent climate history in this area is limited. A warm and wet mid-Holocene climate has been widely reported in the East Asian Monsoon-influenced semiarid areas of northern China [<xref ref-type="bibr" rid="scirp.75149-ref3">3</xref>] [<xref ref-type="bibr" rid="scirp.75149-ref4">4</xref>] . However, other researchers suggest a dry mid-Holocene in the south Ordos Plateau based on multi-proxy data [<xref ref-type="bibr" rid="scirp.75149-ref5">5</xref>] [<xref ref-type="bibr" rid="scirp.75149-ref6">6</xref>] [<xref ref-type="bibr" rid="scirp.75149-ref7">7</xref>] , while another study from the northwestern edge of the Ordos Plateau suggests extremely dry conditions during 8 - 4 ka BP [<xref ref-type="bibr" rid="scirp.75149-ref8">8</xref>] . More high-resolution records are needed to clarify the Holocene climate history for this important region. In this paper, we shed light on regional changes in climate and vegetation by presenting a new pollen record from the Ordos Plateau.</p></sec><sec id="s2"><title>2. Study Site</title><p>Our high-resolution pollen record comes from Bahar Nuur (39˚19'N, 109˚16'E, 1278 m a.s.l.), a seasonally dry lake in the middle of the Maowusu Desert on the arid steppe-dominated Ordos Plateau in Inner Mongolia, China. The site is located on the northwestern margin of land affected by the East Asian monsoon and near the outer limit of the summer monsoon (<xref ref-type="fig" rid="fig1">Figure 1</xref>). Although cold, dry air masses from the north and west dominate this area for most of the year, the East Asian summer monsoon is bringing abundant precipitation to the area during the summer. The mean annual temperature ranges from 7˚C to 9˚C and mean annual precipitation ranges from 200 to 300 mm [<xref ref-type="bibr" rid="scirp.75149-ref9">9</xref>] . Many lakes occupy wind-deflation or river-eroded depressions formed during the late Quaternary [<xref ref-type="bibr" rid="scirp.75149-ref10">10</xref>] . Bahar Nuur Lake formed along an abandoned river channel that was later modified by sand-dune activities. The dry lakebed occupies an area of 26.50 kmP<sup>2</sup>, although presently the lake only contains water seasonally.</p><p>Following the northwest to the southeast precipitation gradient, vegetation on the Ordos Plateau changes from desert steppe, steppe, to forest steppe from the northwest to the southeast (<xref ref-type="fig" rid="fig1">Figure 1</xref>). Specifically, temperate desert, dominated by Chenopodiaceae, occurs in the northwestern most section, whereas temperate Stipa-dominated steppes occur in the southeastern part. The central and southern Ordos Plateau is occupied by temperate Caragana shrubs. Artemisia arenaria and Stipa-dominated steppes are widely distributed throughout the Plateau. Xerophytic plant communities dominated by Agriophyllum squarrosum, Corispermum spp and Salsola spp occupy sandy areas. The modern zonal vegetation around Bahar Nuur is Artemisia arenaria and Stipa-dominated steppes, which also contain Thymus mongolicus, Salsola spp, Astragalus adsurgens, and Ephedra spp [<xref ref-type="bibr" rid="scirp.75149-ref11">11</xref>] .</p><p>While trees are generally rare in the Bahar Nuur area, conifer and broadleaf mixed forests occur in the mountains to the south, west, and north of the study site. Spruce and pine forests are present on the Helan and Daluo Mountains to the west, and the Ziwu and Liupan Mountains to the south, while pine forest occurs on the Yinshan Mountain to the north. All five mountain ranges are located &lt;500 km from the study site; thus the alpine forests may be a source of long-distance-transported pollen to the Bahar Nuur pollen record.</p><p>Both Pinus bungenna (Haploxylon type Pinus) and Pinus tabalaeformit (Diploxylon type Pinus) are present in those mountains near Ordos Plateau. Pinus</p><fig id="fig1"  position="float"><label><xref ref-type="fig" rid="fig1">Figure 1</xref></label><caption><title> Regional vegetation around the Ordos Plateau showing location of the study sites, surface pollen samples and its relative location within China</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/75149x2.png"/></fig><p>bungenna typically grow in cold, arid environment at higher elevations, whereas Pinus tabalaeformit tends to occur in mild, wet environment at lower elevations [<xref ref-type="bibr" rid="scirp.75149-ref12">12</xref>] .</p></sec><sec id="s3"><title>3. Methods</title><p>A 530-cm long core was drilled with a gravitational piston corer in 2003 in the central part of Bahar Nuur. Fossil pollen samples were collected at intervals of ~5 cm and 100 samples were analyzed. Samples for other analyses (grain size, total organic carbon [TOC], and δ<sup>13</sup>C of organic matter) were collected at 2 cm intervals. Ten surface soil samples were collected from some 5 lake shores in the Ordos Plateau to provide modern pollen data in order to improve the interpretation of the fossil pollen spectra regarding pollen sources, transport processes, and bioclimatic representation.</p><p>We take 20 - 30 gram soil for each sample. Following standard palynological procedures [<xref ref-type="bibr" rid="scirp.75149-ref13">13</xref>] , pollen samples were treated with potassium hydroxide, hydro- chloric acid, and hydrofluoric acid to remove organic matter, carbonates, and silicates, respectively. One Lycopodium tablet (Lund University, Batch No. 938934) was added per sample in order to calculate pollen concentrations.</p><p>Pollen types were identified with the aid of the Moore et al. [<xref ref-type="bibr" rid="scirp.75149-ref14">14</xref>] and Wang et al. [<xref ref-type="bibr" rid="scirp.75149-ref15">15</xref>] identification keys. A total of 48 families or genera were identified. More than 300 pollen grains (not including spores) were counted for each sample and pollen percentages were calculated based on the sum of all terrestrial pollen. A pollen percentage diagram including total pollen concentrations was plotted against depth using Grapher 2.0. Cluster analysis (CONISS) was conducted to delineate pollen zones [<xref ref-type="bibr" rid="scirp.75149-ref16">16</xref>] .</p><p>The particle size of bulk samples was measured using a Malvern Co. Ltd. Mastersizer 2000 laser diffraction particle size analyzer. Pretreatment of the samples for particle size analysis included adding (1) H<sub>2</sub>O<sub>2</sub> to remove organic matter, (2) diluted 6N HCl to remove salts, and (3) sodium-hexametaphosphate to disperse the aggregates. The carbonate content was measured with the modified gas evolution method using a Bascomb Calcimeter and total organic matter content was measured with titration method. For organic carbon isotopic analysis (δ<sup>13</sup>C<sub>org</sub>), dried sediment samples were first sieved through meshes of 0.105 mm to remove undecomposed roots. The sieved fine portion was de-carbonated for 24 hours by adding 10% HCl and by repeatedly rinsing with distilled water (until pH ≈ 7). The samples were dried at 50˚C for isotopic analyses. The prepared samples were combusted to produce CO<sub>2</sub>. The combustion-produced CO<sub>2</sub> was trapped in a cold finger of liquid N<sub>2</sub> and then bathed in a trap of −75˚C to gather pure CO<sub>2</sub> [<xref ref-type="bibr" rid="scirp.75149-ref17">17</xref>] .</p><p>Fifteen bulk sediment samples were submitted to Beta Analytic Inc. and the NSF AMS Facility at the University of Arizona for AMS <sup>14</sup>C dating.</p></sec><sec id="s4"><title>4. Results</title><sec id="s4_1"><title>4.1. Modern Pollen Data</title><p>Tree pollen includes Pinus, Quercus, Cupressus, Ulmus, Betula, and others. Mesophilous herb pollen includes Compositate, Artemisia, Gramineae, Urticaceae, Polygonum, Liliaceae, Leguminosae, and others. Xeromorphic herb pollen includes Nitraria, Zygophyllaceae, Ephedra, Chenopodiaceae and others. Wetland plant pollen includes Typhaceae, Cyperaceae, Plantago and Sparganiaceae. Shrub pollen includes Elaeagnaceae and Rosaceae (<xref ref-type="fig" rid="fig2">Figure 2</xref>).</p><p>All modern pollen samples contain relatively high percentages of Haploxylon type Pinus pollen and Diploxylon type Pinus pollen, ranging between ~10% and ~40% (<xref ref-type="fig" rid="fig3">Figure 3</xref>), although, except for a few pine trees planted in this area, there are no pine forests or pine-associated forest-steppes occurring around the lake or in the watershed. The Pinus pollen has most likley probably been transported by wind from the pine-dominated forest-steppes. It is apparent that the Pinus pollen is over-represented in the pollen spectra, confirming the widely-reported over-representation of Pinus pollen in the semi-arid and arid regions of China and Mongolia [<xref ref-type="bibr" rid="scirp.75149-ref18">18</xref>] .</p><p>Despite the dominance of grasses in the steppes and desert steppes in the Ordos Plateau, Gramineae pollen percentages are rather low, being below 10% in seven of the ten surface samples, confirming the under-representation of Gramineae pollen previously reported for the Mongolian steppes [<xref ref-type="bibr" rid="scirp.75149-ref18">18</xref>] . Chenopodiaceae and Artemisia pollen percentages are relatively high. Although there are only scattered Quercus trees but no Quercus forest in the Ordos Plateau, Quercus accounts for most of the broadleaf tree pollen.</p></sec><sec id="s4_2"><title>4.2. Lithology and Chronology</title><p>The basal section (530 - 435 cm) of the core is slightly dark sand. It is overlain by lacustrine sediments consisting of a dark clay (435 - 350 cm), a slightly dark silty-clay (350 - 300 cm), la Carbonate-enriched and clayed varves (300 - 230 cm), and a reddish-brown silty-clay (230 - 80 cm). The top 80 cm is grayish sand (<xref ref-type="fig" rid="fig3">Figure 3</xref>).</p><p>Fifteen AMS <sup>14</sup>C dates were obtained (<xref ref-type="table" rid="table1">Table 1</xref>). All material is bulk. The basal sand (435 - 530 cm) was dated at three depths with <sup>14</sup>C ages from 13,910 &#177; 70 to</p><fig id="fig2"  position="float"><label><xref ref-type="fig" rid="fig2">Figure 2</xref></label><caption><title> Pollen percentage diagram of 10 surface samples collected from lake shores in 0 - 5 m depth</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/75149x3.png"/></fig><fig id="fig3"  position="float"><label><xref ref-type="fig" rid="fig3">Figure 3</xref></label><caption><title> Lithology and Depth-Age model</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/75149x4.png"/></fig><table-wrap id="table1" ><label><xref ref-type="table" rid="table1">Table 1</xref></label><caption><title> AMS dates and the relevant information (AA samples were dated by the NSF AMS Facility at the University of Arizona; Beta samples were dated by Beta Analytic, Inc., USA)</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Lab No.</th><th align="center" valign="middle" >Field No.</th><th align="center" valign="middle" >Depth (cm)</th><th align="center" valign="middle" ><sup>13</sup>C/<sup>12</sup>C (‰)</th><th align="center" valign="middle" >AMS dates</th><th align="center" valign="middle" >Cal. Age B.P.</th><th align="center" valign="middle" >subtract 1950 yr (True age)</th></tr></thead><tr><td align="center" valign="middle" >Beta-181620</td><td align="center" valign="middle" >BN16</td><td align="center" valign="middle" >81</td><td align="center" valign="middle" >−23.8</td><td align="center" valign="middle" >5440 &#177; 40</td><td align="center" valign="middle" >6251 &#177; 34</td><td align="center" valign="middle" >4301 &#177; 34</td></tr><tr><td align="center" valign="middle" >AA56715</td><td align="center" valign="middle" >BN15</td><td align="center" valign="middle" >103</td><td align="center" valign="middle" >−8.5</td><td align="center" valign="middle" >5549 &#177; 42</td><td align="center" valign="middle" >6352 &#177; 38</td><td align="center" valign="middle" >4402 &#177; 38</td></tr><tr><td align="center" valign="middle" >AA56714</td><td align="center" valign="middle" >BN14</td><td align="center" valign="middle" >133</td><td align="center" valign="middle" >−12</td><td align="center" valign="middle" >6538 &#177; 44</td><td align="center" valign="middle" >7461 &#177; 29</td><td align="center" valign="middle" >5511 &#177; 29</td></tr><tr><td align="center" valign="middle" >Beta-181620</td><td align="center" valign="middle" >BN13</td><td align="center" valign="middle" >167</td><td align="center" valign="middle" >−24.2</td><td align="center" valign="middle" >6340 &#177; 40</td><td align="center" valign="middle" >7268 &#177; 48</td><td align="center" valign="middle" >5318 &#177; 48</td></tr><tr><td align="center" valign="middle" >Beta-181619</td><td align="center" valign="middle" >BN11</td><td align="center" valign="middle" >227</td><td align="center" valign="middle" >−24.2</td><td align="center" valign="middle" >7500 &#177; 40</td><td align="center" valign="middle" >8306 &#177; 63</td><td align="center" valign="middle" >6356 &#177; 63</td></tr><tr><td align="center" valign="middle" >Beta-171831</td><td align="center" valign="middle" >BN10</td><td align="center" valign="middle" >250</td><td align="center" valign="middle" >−25.7</td><td align="center" valign="middle" >8130 &#177; 50</td><td align="center" valign="middle" >9090 &#177; 61</td><td align="center" valign="middle" >7140 &#177; 61</td></tr><tr><td align="center" valign="middle" >AA51969</td><td align="center" valign="middle" >BN9</td><td align="center" valign="middle" >283</td><td align="center" valign="middle" >−13.1</td><td align="center" valign="middle" >8631 &#177; 52</td><td align="center" valign="middle" >9608 &#177; 53</td><td align="center" valign="middle" >7658 &#177; 53</td></tr><tr><td align="center" valign="middle" >Beta-171830</td><td align="center" valign="middle" >BN8</td><td align="center" valign="middle" >327</td><td align="center" valign="middle" >−24.9</td><td align="center" valign="middle" >7960 &#177; 40</td><td align="center" valign="middle" >8839 &#177; 106</td><td align="center" valign="middle" >6889 &#177; 106</td></tr><tr><td align="center" valign="middle" >AA51968</td><td align="center" valign="middle" >BN7</td><td align="center" valign="middle" >345</td><td align="center" valign="middle" >−12.4</td><td align="center" valign="middle" >9038 &#177; 57</td><td align="center" valign="middle" >10,208 &#177; 33</td><td align="center" valign="middle" >8258 &#177; 33</td></tr><tr><td align="center" valign="middle" >Beta-171829</td><td align="center" valign="middle" >BN6</td><td align="center" valign="middle" >385</td><td align="center" valign="middle" >−24.8</td><td align="center" valign="middle" >8850 &#177; 40</td><td align="center" valign="middle" >9973 &#177; 133</td><td align="center" valign="middle" >8023 &#177; 133</td></tr><tr><td align="center" valign="middle" >AA51967</td><td align="center" valign="middle" >BN5</td><td align="center" valign="middle" >415</td><td align="center" valign="middle" >−11</td><td align="center" valign="middle" >9303 &#177; 64</td><td align="center" valign="middle" >10,489 &#177; 95</td><td align="center" valign="middle" >8539 &#177; 95</td></tr><tr><td align="center" valign="middle" >AA51966</td><td align="center" valign="middle" >BN4</td><td align="center" valign="middle" >431</td><td align="center" valign="middle" >−10.2</td><td align="center" valign="middle" >9338 &#177; 57</td><td align="center" valign="middle" >10,553 &#177; 86</td><td align="center" valign="middle" >8603 &#177; 86</td></tr><tr><td align="center" valign="middle" >Beta-171828</td><td align="center" valign="middle" >BN3</td><td align="center" valign="middle" >487</td><td align="center" valign="middle" >−23.8</td><td align="center" valign="middle" >13,910 &#177; 70</td><td align="center" valign="middle" >17,162 &#177; 208</td><td align="center" valign="middle" >15,212 &#177; 208</td></tr><tr><td align="center" valign="middle" >AA51965</td><td align="center" valign="middle" >BN2</td><td align="center" valign="middle" >506</td><td align="center" valign="middle" >−3.2</td><td align="center" valign="middle" >27,850 &#177; 280</td><td align="center" valign="middle" >32,405 &#177; 340</td><td align="center" valign="middle" >30,455 &#177; 340</td></tr><tr><td align="center" valign="middle" >AA51964</td><td align="center" valign="middle" >BN1</td><td align="center" valign="middle" >527</td><td align="center" valign="middle" >−6.5</td><td align="center" valign="middle" >24,290 &#177; 537</td><td align="center" valign="middle" >29,133 &#177; 648</td><td align="center" valign="middle" >27,183 &#177; 648</td></tr></tbody></table></table-wrap><p>27,850 &#177; 280 <sup>14</sup>C yr BP. The large age difference between 487 cm (13,910 &#177; 70 <sup>14</sup>C yr BP) and 506 cm (27,850 &#177; 280 <sup>14</sup>C yr BP) perhaps results from a depositional hiatus or an erosion event during the last glacial maximum. The age reversal between 506 cm (27,850 &#177; 280 <sup>14</sup>C yr BP) and 527 cm (24,290 &#177; 537 <sup>14</sup>C yr BP) may be a result of the upward movement of sediments, either by sand reactivation or bioturbation. Three slight age reversals occur at 133 cm, 327 cm, and 385 cm.</p><p>A carbon reservoir age could not be directly calculated. Because the top 80 cm of the core consists of aeolian transported sand, the common linear regression method could not be used, while the complete lack of terrestrial material eliminated the possibility of employing the comparison method. Therefore, the AMS dates were corrected by applying previously published regional data, which show that the radiocarbon reservoir in the arid-semiarid western China is generally high, though variable [<xref ref-type="bibr" rid="scirp.75149-ref19">19</xref>] [<xref ref-type="bibr" rid="scirp.75149-ref20">20</xref>] [<xref ref-type="bibr" rid="scirp.75149-ref21">21</xref>] , with values ranging from 1000 - 2000 yr in the Ordos plateau [<xref ref-type="bibr" rid="scirp.75149-ref19">19</xref>] and values ranging from 2590 to 4340 yr in the Qaidam Basin of the Tibetan Plateau [<xref ref-type="bibr" rid="scirp.75149-ref22">22</xref>] . Closer to the site, we recently obtained a radiocarbon reservoir of 1950 years from Qigai Nuur, only 1 km from Bahar Nuur. This is consistent with reservoir values of 2000 yr from Daihai [<xref ref-type="bibr" rid="scirp.75149-ref23">23</xref>] about 200 km to the north, and 1935 yr from Lake Gouchi about 100 km to the west [<xref ref-type="bibr" rid="scirp.75149-ref24">24</xref>] . Accodingly, assuming a reservoir effect of 1950 yr for the Bahar Nuur, we subtracted 1950 yr from all the AMS dates obtained from the core. All corrected AMS dates were converted into calibrated ages using the INTCAL program [<xref ref-type="bibr" rid="scirp.75149-ref25">25</xref>] , and a linear regression, best-fit calibrated age-depth model was constructed after eliminating the three reversed age data. A linear relationship (R<sup>2</sup> = 0.9521) exists among the 9 AMS dates within the lacustrine section of the core (435 - 80 cm), suggesting that the age model adequately captures the chronology. The linear relationship is also supported by the relatively uniform particle size distribution within the section of lacustrine sediments. The average sediment rate for this section is 0.081 cm/yr.</p></sec><sec id="s4_3"><title>4.3. Pollen Stratigraphy and Associated Proxy Data</title><p>Because pollen is absent or rare in the sand intervals at the bottom and at the top of the core, fossil pollen data are only presented for the lacustrine section from 435 - 80 cm. Seven pollen assemblage zones, including the top and bottom non- polleniferous zones, are recognized for the core, based on visual inspection and CONISS results (<xref ref-type="fig" rid="fig4">Figure 4</xref>).</p><p>Zone 1 (530 - 435 cm; before 8.6 ka BP), corresponding with the basal sand, contains little or no pollen. Here the TOC is less than 2% and the δ<sup>13</sup>C of organic matter is relatively low (mostly −24‰ to −26‰). Carbonate values are &lt;8%.</p><p>Zone 2 (435 - 350 cm; 8.6 - 8.2 ka BP), which roughly corresponds with the clay unit, is characterized by low pollen percentages for Pinus pollen and high percentages of Gramineae. Pinus pollen are &lt;80%. Quercus is &lt;8%. Chenopodiaceae is high with average value 5%. Ephdra fluctuate within 1% - 5%. Gramineae pollen percentage peaks in this zone. Pollen concentration is less than 20,000</p><fig id="fig4"  position="float"><label><xref ref-type="fig" rid="fig4">Figure 4</xref></label><caption><title> (a) Pollen percentage diagram from Bahar Nuur. (b) Curves for other proxy parameters from the Bahar Nuur core (data from [<xref ref-type="bibr" rid="scirp.75149-ref17">17</xref>] )</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/75149x5.png"/></fig><p>grains/g. TOC increases abruptly to 4% - 6% and remains high throughout the lacustrine section. Mean grain size is uniformly low (&lt;50 μm) in this clay unit, whereas δ<sup>13</sup>C<sub>org</sub> is generally high (−22‰ to −24‰). Carbonate increases sharply to 20% - 30%.</p><p>Zone 3 (350 - 300 cm; 8.2 - 7.8 ka BP), corresponding with the silty-clay unit, is marked by a distinct increase in Quercus pollen at the base. The pollen percentages of Ephedra remain high, while the pollen percentage of Pinus increases slightly. Quercus fluctuate within 2% - 10%. Chenopodiaceae is around 3% - 4%. Ephdra is about 2% - 3%, Gramineae pollen percentage &lt;10%. Pollen concentration fluctuates within 8000 - 22,000 grains/g. Mean grain size increases slightly and Carbonate remains high.</p><p>Zone 4 (350 - 220 cm; 7.8 - 6.3 ka BP), which roughly corresponds with the laminated calcareous clay, is marked by stable pollen percentages. Pinus pollen values are around 75% - 80%. Quercus fluctuate within 2% - 10%. Chenopodiaceae and Ephdra reach their highest values, while Gramineae values decline slightly. Pollen concentration fluctuates within 8000 - 18,000 grains/g. Mean grain size is very small and carbonate is high. Charcoal concentration is around 4000 - 6000 grains/g. δ<sup>13</sup>C<sub>org</sub> values fluctuate in this zone but on the whole decline slightly.</p><p>Zone 5 (220 - 100 cm; 6.3 - 4.4 ka BP), corresponding with the silty-clay, is characterized by increasing pollen percentages of Pinus, while Quercus pollen reaches its maximum abundance. Rosaceae increases, reaching 2%. The steppe component (Chenopodiaceae, Ephdra, Atermisia and Gramineae) pollen percentage decline while wetland taxa (i.e. Spargania) increase. Pollen concentration values are highest in this zone. Charcoal concentration fluctuates within 4000 - 10,000 grains/g. Mean grain size is slightly larger than in the previous zone but is variable. δ<sup>13</sup>C<sub>org</sub> drops sharply near the top of this zone while TOC increases. Carbonate fluctuates in this zone but remains relatively high.</p><p>Zone 6 (100 - 80 cm; 4.4 - 4.3 ka BP) roughly corresponds with the silty-clay. Pinus pollen increases to maximum percentages while almost all other pollen taxa abruptly decrease or disappear, with only Gramineae remaining stable. Pollen concentrations also decline sharply in this zone. Mean grain size increases dramatically while TOC decreases sharply. Charcoal concentration declines. δ<sup>13</sup>C<sub>org</sub> recovers from the crash but remains low and Carbonate is low.</p><p>Zone 7 (80 - 0 cm; after 4.3 ka BP), corresponds with the top eolian sand unit. Pollen grains were not plotted due to their scarcity.</p></sec></sec><sec id="s5"><title>5. Regional Comparison</title><sec id="s5_1"><title>5.1. Early-Holocene</title><p>The non-polleniferous sand deposited before 8.6 ka BP suggests that no lake existed and that the vegetation was locally very sparse before 8.6 ka BP. The climate was probably dry, or the dry lakebed was not conducive to pollen deposition and preservation. Although an early Holocene climate optimum was documented for Midiwan [<xref ref-type="bibr" rid="scirp.75149-ref6">6</xref>] during 11.5 - 8.3 ka BP following a humid phase from 16.3 and 8.87 ka BP in Yanhaizhi [<xref ref-type="bibr" rid="scirp.75149-ref8">8</xref>] , a number of studies have shown that along the northern margin of the East Asian monsoon precipitation was low in the early Holocene, increasing after 8 ka BP [<xref ref-type="bibr" rid="scirp.75149-ref4">4</xref>] [<xref ref-type="bibr" rid="scirp.75149-ref26">26</xref>] .</p><p>At ~8.6 ka BP, the core lithology changes from sand to clay, and pollen becomes much more abundant. Charcoal concentrations also increase significantly. The abrupt onset of lacustrine sedimentation suggests that the lake formed rather quickly. This indicates a rapid climatic change towards wetter conditions, and denser vegetation. This is supported by dramatic increases in TOC and a sharp decrease in mean grain size.</p></sec><sec id="s5_2"><title>5.2. Middle Holocene</title><p>The presence of a lake from ~8.6 to 4.3 ka BP, as indicated by contonious lacustrine sedimentation, implies that the mid-Holocene was a more humid than the present. Between ~8.6 and ~7.8 ka BP (Zone 2 and Zone 3), during the early phase of this wet period the vegetation around Bahar Nuur was a grass dominated steppe with some xerophytic herbs and shrubs such as Chenopodiaceae and Ephedra.</p><p>From ~7.8 to ~6.3 ka BP (Zone 4), although steppe vegetation still dominates, many other types of plants appear and persist. This sediment consists of alternating varves, composed of black (organic matter-rich) and white (carbonate) couplets. The formation of varves requires strikingly different seasonal conditions and lake depth deep enough to permit stable bottom water [<xref ref-type="bibr" rid="scirp.75149-ref27">27</xref>] . The white couplet suggests that the summer temperatures were high enough to drive evaporation-generated carbonate deposition. The fine particle size, indicating high lake levels, suggests that warm and humid conditions prevailed during this period.</p><p>Quercus (Quercus liaotungensis) populations reach their greatest abundance between ~6.3 and ~4.4 ka BP (Zone 5), indicating humid conditions. It is more humid than zone 4. The slight but sustained increase in Rosaceae pollen indicats that shrubs such as Caragana also increased in abundance. The increase in trees and shrubs occurred at the expense of the xerophytic plants, as indicated by a concomitant decrease in the pollen percentages of Gramineae, Chenopodiaceae, and Ephedra. The general decrease in δ<sup>13</sup>C<sub>org</sub> values from zone 2 to zone 5 also supports this scenario, as C3 plants (including most trees) have much lower (i.e., more negative) δ<sup>13</sup>C values than C4 plants (including many desert grasses and herbs). As a result, the vegetation reached its highest density during this period, as evidenced by maximum pollen concentration values for this zone. High charcoal concentrations in this zone are also consistent with maximum biomass, which provide more fuels for fires. The increase in wetland plants such as Spargania also indicate humid environment. The pollen evidence indicates that the Holocene Optimum occurred in this area during Zone5 (~6.3 to ~4.4 ka BP).</p></sec><sec id="s5_3"><title>5.3. Late Holocene</title><p>Quercus pollen declined abruptly after ~4.4 ka BP, as did charcoal concentrations and pollen for most other taxa. These changes signify a return to harsher climate conditions, leading to a more open vegetative landscape. The change from lacustrine sedimentation to deposition of eolian sand after ~4.3 ka BP indicates that the lake either dried up or became ephemeral during the late Holocene, thereby ending the pollen record. Most records from area dominated by the Asian monsoon show markedly lower effective moisture in late Holocene due to the weak summer monsoon [<xref ref-type="bibr" rid="scirp.75149-ref28">28</xref>] .</p></sec><sec id="s5_4"><title>5.4. Regional Comparison of Mid-Holocene Climate Changes</title><p>To put the reconstructed vegetation history of Bahar Nuur in a regional perspective, we reviewed other records in or near the Ordos Plateau. At the Dadiwan and Sujiawan sections in the western part of the Chinese Loess Plateau, deciduous forest of high density and diversity dominated by oaks and birch that existed from 6.56 to 5.79 ka <sup>14</sup>C yr BP were replaced by pine-dominated forest-steppe during 5.79 - 4.95 ka BP (<sup>14</sup>C age), then degraded into elm-dominated sparse forest-steppe during 4.954 ka BP (<sup>14</sup>C age). The vegetation and climate conditions deteriorated after 4 ka<sup> </sup>BP (<sup>14</sup>C age), as forest was replaced by steppe. Thus, the period of maximum warmth and humidity prevailed between 6.56 and 4.95 ka BP (<sup>14</sup>C age) in the western Loess Plateau [<xref ref-type="bibr" rid="scirp.75149-ref29">29</xref>] . However, the pollen- based proxy records from Midiwan [<xref ref-type="bibr" rid="scirp.75149-ref5">5</xref>] [<xref ref-type="bibr" rid="scirp.75149-ref6">6</xref>] suggest that a desert-grassland interval occurred between 7.5 and 4.5 ka (<sup>14</sup>C age) with a relatively warm climate in Ordos Plateau. Corresponding lithology is silty peat with a large amount of plant residues and freshwater fossil mollusks [<xref ref-type="bibr" rid="scirp.75149-ref6">6</xref>] which indicates humid environment. Furthermore, organic carbon content is relatively high compared with next zone. Although pollen concentration is low, Chenopodiaceae and Ephedra pollens are much less than Artemsia and Composite pollens. At the TYG section to the southeast of Bahar Nuur, a warm and humid Holocene Optimum occurred during between ~7.7 and ~5.6 ka BP, as inferred from dune building as well as pollen assemblages [<xref ref-type="bibr" rid="scirp.75149-ref30">30</xref>] . Our data from Bahar Nuur show lush mid-Holocene vegetation in the central Ordos Plateau, with the most mesic conditions occurring around 6.3 ~ 4.4 ka BP. To the west, records from Lake Yanhaizi suggest that the mid-Holocene (8.0 ~ 4.3 ka BP) was arid in Inner Mongolia, with low TOC and high sand sediment [<xref ref-type="bibr" rid="scirp.75149-ref9">9</xref>] . Further to the northeast, pollen records and pollen- based paleoclimatic reconstruction from Daihai in Inner Mongolia suggest that the mean July temperature was 2˚C ~ 3˚C higher and the precipitation was 100 ~ 180 mm higher than present from ~7.9 ~ 4.45 ka BP [<xref ref-type="bibr" rid="scirp.75149-ref26">26</xref>] . At Diajiaohaizi in Daiqingshan, pollen records suggest a generally warm and humid mid-Holo- cene. Temperature was 1˚C - 2˚C higher than today and precipitation was 150 - 200 mm more than today during 6.9 ~ 6.3 ka BP [<xref ref-type="bibr" rid="scirp.75149-ref31">31</xref>] .</p><p>To date, only the Yanhaizi site clearly documents a dry mid-Holocene, contra- dicting records from most other sites in the Ordos Plateau and Chinese Loess Plateau. Since lakes Yanhaizi, Barhar Nuur and Diahai are located in the northwestern margin of Asian monsoon, precipitation levels in this area are closely related to variations in the strength of Asian summer monsoon. We compare our records with Northern Hemisphere insolation and the δ<sup>18</sup>O record from Dongge cave, which is an excellent proxy for Asian monsoon strength.</p><p>The Asian monsoon is strong before ~4.4 ka BP and weak after ~4 ka BP as indicated by the Dongge cave δ<sup>18</sup>O proxy record, corresponding to lush vegetation from 6.3 - 4.4 ka BP and sparse vegetation after ~4.2 ka BP at both Barhar Nuur and Daihai. Corresponding to the dry event that occurred at ~4.2 ka BP, vegetation deteriorated sharply in both Barhar Nuur and Daihai while a weak Asian monsoon event occurred in Dongge cave. This severe climatic deterioration was synchronous with a climatic event during the late third millennium BC identified in many Northern Hemisphere sites [<xref ref-type="bibr" rid="scirp.75149-ref32">32</xref>] , marking the ending of Holocene Optimum. Wu and Liu [<xref ref-type="bibr" rid="scirp.75149-ref32">32</xref>] suggest that this climatic change may have played a very important role in the collapse of Neolithic cultures around the Central Plain of China. A humid mid-Holocene can be inferred from <xref ref-type="fig" rid="fig5">Figure 5</xref>, as the Asian monsoon was relatively strong, and broadleaves tree pollen and pollen concentration are high in both Barhar Nuur and Daihai during 6.3 ~ 4.4 ka BP, reflecting denser vegetation and a humid mid-Holocene. This humid mid-Holocene is also echoed in Qinghai Lake which is also located in north margin of Asian monsoon. According to multi-proxy analysis, Shen presents a warm and wet period between 14.1 ka BP and 4.5 ka BP in Qinghai Lake area. After that, the climate gradually became colder and drier [<xref ref-type="bibr" rid="scirp.75149-ref20">20</xref>] .</p></sec><sec id="s5_5"><title>5.5. Discussion on Forcing Mechanisms</title><p>It has been suggested that variation in the strength of the East Asian monsoon is controlled by changes in summer solar radiation in the Northern Hemisphere on glacial-interglacial timescales [<xref ref-type="bibr" rid="scirp.75149-ref33">33</xref>] . Holocene solar insolation peaked at ~10 ka BP in northern semi-sphere [<xref ref-type="bibr" rid="scirp.75149-ref34">34</xref>] . However, the existence of substantial ice</p><fig id="fig5"  position="float"><label><xref ref-type="fig" rid="fig5">Figure 5</xref></label><caption><title> Holocene climate comparison. A: lithology and pollen data in Barhar Nuur; B: Solar insolation at 40˚N (Berger and Loutre, 1991) [<xref ref-type="bibr" rid="scirp.75149-ref34">34</xref>] ; C: Dongge cave speleothem (Wang et al., 2005) [<xref ref-type="bibr" rid="scirp.75149-ref35">35</xref>] ; D: Daihai trees pollen [<xref ref-type="bibr" rid="scirp.75149-ref26">26</xref>] </title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/75149x6.png"/></fig><p>masses in the northern high latitudes and remnant ice sheets may have hampered the Asian continent from warming up during the early Holocene, resulting in a cold, dry early Holocene in the Ordos Plateau. Oceans need much more energy to warm up than the land. Tropical Holocene SST increased steadily from ~10 ka to ~6 ka BP [<xref ref-type="bibr" rid="scirp.75149-ref36">36</xref>] . According to the δ<sup>18</sup>O proxy record from Dongge cave, the Asian monsoon which was relatively strong until about 4.4 ka BP, would have kept monsoon precipitation levels high during mid-Holocene, particularly given the enhanced evaporation on west Pasific which effectively injected more water vapor into the East Asian monsoon system during ~10 ka to ~6 ka BP [<xref ref-type="bibr" rid="scirp.75149-ref3">3</xref>] . The warmer and wetter climate associated with the Holocene Climatic Optimum lead to denser vegetation cover, which might have further promoted the Optimum through the feedback mechanism related to the air-vegetation-soil coupling under the denser vegetated wetter conditions [<xref ref-type="bibr" rid="scirp.75149-ref3">3</xref>] . It is plausible that the humid mid-Holocene is a lagged response to Asian monsoon and peak summer insolation in the Northern Hemisphere. When summer insolation largely decreased after 6 ka BP and Asian monsoon was weak in late Holocene, it results in a dry late Holocene in the Ordos Plateau.</p><p>The dry mid-Holocene record from Yanhaizi Lake is inconsistent with the wet mid-Holocene extensively documented in other regional sites. The effective soil moisture during the mid-Holocene was probably low in Yanhaizi area where the increase in evaporation resulting from rising temperatures might have exceeded the overall increase in precipitation, leading to an increase in climatic aridity and a decrease in effective soil moisture. However, we find no evidence for a dry mid-Holocene in the Ordos Plateau. More work is required to understand these regional differences.</p></sec></sec><sec id="s6"><title>6. Conclusion</title><p>We analyze 100 pollen samples from Bahar Nuur Lake in order to reconstruct the history of Holocene environment change in the Ordos Plateau, with a chronology based on 15 AMS dates. Before 8.6 ka BP, the climate was probably dry resulting in a sparsely vegetated environment. From 8.6 - 7.8 ka BP, the site was a grass-dominated arid steppe. From 7.8 - 6.3 ka BP, the climate was warm and humid resulting in a steppe. From 6.3 - 4.4 ka BP, climate was probably warm and humid, with a sharp increase in forest taxa, identifying this interval as the Holocene Climatic Optimum. From 4.4 - 4.3 ka BP, the climate dried significantly, with the site becoming an arid steppe and after ~4.3 ka BP, climate became much drier, with the lake transforming into desert steppe. Regional records generally support the view of a humid mid-Holocene prevailed in the Ordos Plateau. We suggest that the mid-Holocene Climatic Optimum occurred in the Ordos Plateau as a delayed response to high insolation in the Northern Hemisphere, as transferred by the Asian monsoon.</p></sec><sec id="s7"><title>Acknowledgements</title><p>The authors thank: Chinese Nature Science Foundation for the grant (No.403- 31012); US. National Science Foundation grants (ATM-0402475); Guizhou Provincial Science and Technology for the grant (2013-2273); Guizhou Provincial Education Department for excellent talent foundation (2013-151). Thanks to Prof. Kam-biu Liu, Prof. Zhao-dong Feng, Prof. Xinqing Lee<sup> </sup>P, Prof. Yu-zhen Ma. They gave much instruction.</p></sec><sec id="s8"><title>Cite this paper</title><p>Huang, C.Q. and Guo, L.L. (2017) Pollen Evidence of a Hu- mid Mid-Holocene Climate from Bahar Nuur, Inner Mongolia. 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