Diets are known to play a major role in the development of many non-communicable diseases such as colon cancer. Thus, dietary compounds are of special relevance for improving the body’s defenses against such diseases. Information about the role of diet has come from many observational studies around the world across cultures and geographical divides [1] [2] . Foods such as beans are rich in carbohydrate, low in fat and contain large amount of fibre which help attract water to the stool and enhance transit time of waste through the colon [3] [4] . This may help to combat constipation, colon cancer, and other conditions that afflict the digestive tract. Despite the strong relationship of dietary habits to risk of colorectal cancer (CRC), epidemiologic studies are generally insufficient to conclude dry beans decrease CRC risk, although there are some suggestions of a protective effect [5] - [8] .

In general, the current treatment options for colorectal cancer are surgery, chemotherapy, and biological therapies. The majority of patients with early-stage colorectal cancer will undergo surgery to remove as much of the tumor as possible [9] - [17] . Many people with colorectal cancer initially respond to chemotherapy, but unfortunately, in the majority of cases the disease eventually progresses after first-line treatment. Several types of biological therapy are available to treat metastatic colorectal cancer including anti-angiogenics and the Epidermal Growth Factor Receptor (EGFR) inhibitors. Most often they are given in combination with chemotherapy [10] . Radiotherapy is not often used to treat metastatic colorectal cancer due to side effects, although it can be used after surgery to destroy any residual cancer cells [11] .

Cost effectiveness analyses of the many interventions (primary prevention, screening or treatment) for reducing the burden of colorectal cancer have usually been restricted to developed country settings and with often considerable variation in the analytical methods used. This limits the value of the existing literature to inform colorectal cancer control policies in low to middle income country settings such as Nigeria. Assessment of costs and effects of different strategies can help guide decisions on the allocation of resources across interventions, as well as between interventions for colorectal cancer. Hence the aim of this present work is to know if the local unexploited wild beans would reverse incidence of colon cancer induced by Dextran Sodium Sulphate (DSS) in wister albino rats and compare the effect with common edible beans.

Dry, wild bean―Sphenostyles stenocarpa (Otili), Cajanus cajan (Feregede), Phaseolus lunatus (Pakala), were sourced from the bush of Ado Ekiti environ while the wild type-Mucuna cochindunum (Mucuna), and edible type bean―Phaseolus vulgaris tagged, IT845-2246-4 were brought from International Institute of Tropical Agriculture (IITA), Ibadan Nigeria. They were collected whole, dry and naked (i.e. without pods), with divers colors (off white, gold, navy blue, black and brown respectively). Authentication was carried at the herbarium of the Plant Science Department, Ekiti State University Ado Ekiti before the dried seeds were pulverised.

Sixteen male and twelve female albino rats were used in this study. Their weights ranged between 60 g to 100 grams. The rats were fed ad-libitum with pulverized bean samples and 5 mg/Kg of Dextran Sodium Sulphate (DSS) dissolved in water for seven days to induce colonic inflammation. An average of 15 mL of the DSS-water was given to the singly caged rat under group A-E daily. They are Otili, Feregede, Pakala, Mucuna and edible bean cultivar IT845-2246-4 groups respectively. However the Control Group was fed with rat chow and 15 mL of 5 mg/Kg body weight DSS treated water, while the Basal group was fed with rat chow and normal saline daily.

Experimental studies have been conducted specifically examining the relationship of dry bean consumption to chemically-induced colon cancer in rats. For instance, Hughes et al., [14] fed rats diets containing either pinto beans (59% wt/wt) or casein as the protein source. They found that feeding pinto beans inhibited colon tumor incidence by 52% (50% in casein-fed animals vs. 24% in bean-fed animals) and significantly reduced the number of tumors that developed (1.0 ± 0.0 vs. 2.5 ± 0.6 tumors/ tumor bearing animal). In a similar design, Hangen and Bennink [15] also reported a protective effect of dry beans on experimental colon cancer. They found that feeding either black beans or navy beans (75% wt/wt) inhibited colon cancer by ~57%, and similar to Hughes et al., [14] , bean-fed rats also developed fewer tumors (1.0 ± 0.17 vs. 2.2 ± 1.2 tumors/tumor bearing animal). It was on this premise that this present work was set out following the Morton and Griffiths [16] groundwork laid for developing a set of observations for assessing behavioral and physiological changes in laboratory animals.

Significant changes were observed in all physiological categories but observation recorded in day 1 was insignificant in day 1. As shown in Figure 1 responses to the amount of food consumed by each rat group were encouraging except in group fed with mucuna. The group fed with mucuna experienced a notable reduction in weight just at day 2. This reduction definitely was induced by a loss of appetite and a reduction in food and water-treatment consumed (Figure 1). While feregede group experienced a slight reduction in weight and heart beat rate although it remained active all day round. Pakala and feregede groups had similar effects with a slight decrease in weight and food consumed while group fed with common edible bean had no noticeable weight

change between day 1 and 2, but experienced a slight decrease in weight on the 5^th day of the experiment (Figure 2) At day 4 group fed with Otili experienced a sharp reduction in weight on the fourth day of the experiment this was maintained over the fifth day before decreasing to 75.7 on day 7 of the experiment. This depreciation in weight is also relative to low amount of food consumed at this period.

Macroscopic result (Table 1) before sacrifice on seventh day shows that control had the highest % tumor burden of 64.9% and Otili group had the lowest with 14.3% along with B with 15%. Pakala group had a relatively high percent of 60.3%, preceded by group fe with common edible bean with 58.4% tumor burden. Death was recorded for mucuna group before the seventh day so this measurement was not ascertained for them. Further histological assessment showed dyspepsia observed on the control group ( Picture 1 ). Though the dyspepsia was mild in the Otili group ( Picture 2 ), while that of feregede group was also moderate ( Picture 3 ), however dyspepsia in group fed with pakala ( Picture 4 ) appeared more severe compared to otili, feregede and edible bean IT845-2246-4 ( Picture 5 ). Maximum effect of the DSS was found to be in the Mucuna group with profound lesions showing on the tissues ( Picture 6 ).

Picture 1. Result of histological examination of control group wistar rats.

Picture 2. Result of histological examination of rats fed with Otili.

Picture 3. Result of histological examination of rats fed with feregede.

Picture 4. Result of histological examination of rats fed with pakala.

Picture 5. Result of histological examination of group E rats.

Picture 6. Result of histological examination ofrats fed with mucuna.

In this present study, it is justifiable to say that group of rats fed with Mucuna had the least enduring ability to the DSS treatment being induced into it. Rats fed with Otili and Feregede expressed higher resistibility ability to the proliferation of cancer cells.

The present project was partly funded by Tet Fund Individual Based Research grant 2015-2016 session.

O. A. Awoyinka,A. Ileola,C. N. Imeoria,A. E. Omonisi,F. C. Oladele,M. F. Asaolu, (2016) Comparative Study on Nigerian Wild and Edible Beans in Reversing Incidence of Colon Cancer in Albino Rats. Open Access Library Journal,03,1-7. doi: 10.4236/oalib.1102708