<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">JACEN</journal-id><journal-title-group><journal-title>Journal of Agricultural Chemistry and Environment</journal-title></journal-title-group><issn pub-type="epub">2325-7458</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/jacen.2016.52007</article-id><article-id pub-id-type="publisher-id">JACEN-66240</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Chemistry&amp;Materials Science</subject><subject> Earth&amp;Environmental Sciences</subject></subj-group></article-categories><title-group><article-title>
 
 
  Influence of the Storage Duration at Different Temperatures on the Concentrations of Extractable Inorganic Soil Nutrients
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>amal</surname><given-names>T. Elfaki</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Ahmed</surname><given-names>M. Nour</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Magboul</surname><given-names>M. Sulieman</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Mushtaha</surname><given-names>E. Ali</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib></contrib-group><aff id="aff2"><addr-line>Agricultural Research Corporation, Wad Medani, Sudan</addr-line></aff><aff id="aff3"><addr-line>Department of Soil and Environment Sciences, University of Khartoum, Khartoum, Sudan</addr-line></aff><aff id="aff4"><addr-line>General Administration of Natural Resource and Sustainable Development, Khartoum, Sudan</addr-line></aff><aff id="aff1"><addr-line>Faculty of Agriculture, Nile Valley University, Atbara, Sudan</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>magboul@uofk.edu(MMS)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>29</day><month>04</month><year>2016</year></pub-date><volume>05</volume><issue>02</issue><fpage>66</fpage><lpage>72</lpage><history><date date-type="received"><day>18</day>	<month>February</month>	<year>2016</year></date><date date-type="rev-recd"><day>accepted</day>	<month>2</month>	<year>May</year>	</date><date date-type="accepted"><day>5</day>	<month>May</month>	<year>2016</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
  The influence of the short storage periods at different temperatures on the concentrations of extractable soil cations (Ca
  <sup>2+</sup>, Mg
  <sup>2+</sup>, Na
  <sup>+</sup> and K
  <sup>+</sup>) and anions (Cl
  <sup>-</sup>, SO
  <sub>4</sub>
  <sup style="margin-left:-6px;">-</sup> and PO
  <sub>4</sub>
  <sup style="margin-left:-6px;">-</sup> ) has been investigated in nine soil samples from Nile river terraces at River Nile State, North of Sudan (17.82289 to 17.82389N and 33.99974 to 34.02127E). Each soil extract is divided into three treatments: i) control (immediately analyzed); ii) storage for 10 days and; iii) storage for 30 days. Each treatment is replicated three times: i) storage at 10&#176;C; ii) storage at ambient laboratory temperature (25&#176;C) and; iii) storage at 45&#176;C in incubator. Statistical analysis of results reveals that significant difference are found at level (P &lt; 0.05) for K
  <sup>+</sup>, Mg2
  <sup>+</sup>, Ca2
  <sup>+</sup>, Cl
  <sup>-</sup>, SO
  <sub>4</sub>
  <sup style="margin-left:-6px;">-</sup> and PO
  <sub>4</sub>
  <sup style="margin-left:-6px;">-</sup> (0.043, 0.002, 0.001, 0.021, 0.004 and 0.001) respectively at 25&#176;C and 45&#176;C and storage periods of 10 and 30 days. In contrast, significant difference is also found at level (P &lt; 0.001) for soluble calcium, magnesium and sulphate. In addition, results also reveal that SO
  <sub>4</sub>
  <sup style="margin-left:-6px;">-</sup> concentrations are significantly decreased when the storage period exceeds 10 days and temperature more than 25&#176;C. Depending upon our study results; we conclude that, all extractable inorganic nutrients are clearly affected by storage periods at various temperatures, exception of Na
  <sup>+</sup>. Most cations and anions are increased significantly with increased of storage period and temperatures. We therefore highly recommend that the extractable inorganic soil nutrients should be rapidly analyzed in order to obtain accurate results; otherwise, the time between extraction and analysis should be carefully recorded which may help considerably interpreting data from various studies.
 
</p></abstract><kwd-group><kwd>River Nile State</kwd><kwd> Extractable Soil Cations</kwd><kwd> Storage Period</kwd><kwd> Inorganic Nutrients</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>Soil chemistry changes through the time, as biological and chemical processes break down or combine compounds through the time. These processes change once the soil is removed from its natural ecosystem (flora and fauna that penetrate the sampled area) and environment (temperature, moisture, and solar light/radiation cycles). As a result, the chemical composition analysis accuracy can be improved if the soil is analyzed soon after its extraction [<xref ref-type="bibr" rid="scirp.66240-ref1">1</xref>] .</p><p>The soil-fresh sample received in the laboratory should be analyzed directly after sampling for determination of nitrate, nitrite and ammonium. The chemical changes in the soil can be slowed during storage and transportation by freezing it. Air-drying can also preserve the soil sample for many months. Lag time between field sampling and analysis must be minimized. Otherwise, storage time will inevitably introduce an additional factor influencing analysis results [<xref ref-type="bibr" rid="scirp.66240-ref2">2</xref>] .</p><p>Soil samples are often store before starting the physiochemical properties analysis. This can affect subsequent concentrations of extractable inorganic nutrients, including <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x12.png" xlink:type="simple"/></inline-formula> and <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x13.png" xlink:type="simple"/></inline-formula> [<xref ref-type="bibr" rid="scirp.66240-ref3">3</xref>] , K<sup>+</sup> [<xref ref-type="bibr" rid="scirp.66240-ref4">4</xref>] and <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x14.png" xlink:type="simple"/></inline-formula> [<xref ref-type="bibr" rid="scirp.66240-ref5">5</xref>] . Moreover, recent study reports that, at 10˚C the values of soluble potassium (K<sup>+</sup>), calcium (Ca<sup>2+</sup>), and magnesium (Mg<sup>2+</sup>) are increased to 10% [<xref ref-type="bibr" rid="scirp.66240-ref6">6</xref>] .</p><p>Sample handling and storage are more important aspects of soil analysis than generally recognized. [<xref ref-type="bibr" rid="scirp.66240-ref7">7</xref>] presents data indicating problems associated with drying and storing soil samples and changes that occur under various sample and storage conditions.</p><p>In this study, we assessed the influence of short storage periods at different temperatures on the concentrations of extractable soil cations (Ca<sup>2+</sup>, Mg<sup>2+</sup>, Na<sup>+</sup> and K<sup>+</sup>) and anions (Cl<sup>−</sup>, <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x15.png" xlink:type="simple"/></inline-formula> and<inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x16.png" xlink:type="simple"/></inline-formula>) in soils of the Nile river terraces at River Nile State, North of Sudan. We tested the hypotheses that i) extractable inorganic nutrients concentrations would be significantly change during different storage periods as well as different temperatures; ii) extractable inorganic nutrients concentrations would be positively correlated with soil formed at different time but derived from similar parent materials; iii) chloride (Cl<sup>−</sup>) and sulphate (<inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x17.png" xlink:type="simple"/></inline-formula>) concentrations would be most changed during storage periods at different temperatures.</p></sec><sec id="s2"><title>2. Materials and Methods</title><sec id="s2_1"><title>2.1. Site Description</title><p>The study area is located in North of Atbara city, River Nile State, Sudan, with an altitude of (336 - 358 meters) above sea level. The study area covered about 8000 ha and is located where the coordinate is at 17.82289 to 17.82389N and 33.99974 to 34.02127E. According to [<xref ref-type="bibr" rid="scirp.66240-ref8">8</xref>] , the study area falls within the arid climatic zone. The average annual rainfall varies from 0 to &lt; 100 mm. Mean maximum temperature of the hottest months (May and June) is 43˚C. Mean minimum temperature of the coldest month (January) is less than 13˚C. The mean annual relative humidity ranges between 15% to 21% (January to February), less than 15% (March to June). The predominant natural vegetation in the study area consists of the following species: Tundub (Capparis decidua), Seyal (Acacia tortilis), Usher, Musket (Prosopis chilensis), Heglig (Balanites aegypiaca) and Seder (Zizyphus spina-christi). The calculated soil temperature regime is hyperthermic and soil moisture regime is arridic. According [<xref ref-type="bibr" rid="scirp.66240-ref9">9</xref>] , the soils of the study area belong within Entisols and Aridisols orders.</p></sec><sec id="s2_2"><title>2.2. Soil Sampling and Preparation</title><p>Nine soil samples were collected from different Nile river terraces at River Nile State, North of Sudan (<xref ref-type="table" rid="table1">Table 1</xref>). At each site, approximately 5 kg of soil sample was collected from the depth of 0 - 30 cm using an auger and kept in a plastic bag. In the laboratory, soil samples were air-dried and passed through a 2 mm mesh sieve to obtain the fine earth fraction. To estimate the influence of storage period at different temperatures on the concentrations of soluble cations and anions, a 1:5 soil suspension was shaken for 30 minutes, and then extracted</p><table-wrap id="table1" ><label><xref ref-type="table" rid="table1">Table 1</xref></label><caption><title> Description of samples sites in the study area and some physical and chemical properties</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Site No.</th><th align="center" valign="middle"  rowspan="2"  >Description</th><th align="center" valign="middle"  colspan="2"  >Location</th><th align="center" valign="middle"  rowspan="2"  >Elevation (m-a.s.l)</th><th align="center" valign="middle"  colspan="3"  >Partial Size Distribution%</th><th align="center" valign="middle"  rowspan="2"  >Texture Class</th><th align="center" valign="middle"  rowspan="2"  >CaCO<sub>3</sub><sub> </sub> (%)</th></tr></thead><tr><td align="center" valign="middle" >N</td><td align="center" valign="middle" >E</td><td align="center" valign="middle" >Sand</td><td align="center" valign="middle" >Silt</td><td align="center" valign="middle" >Clay</td></tr><tr><td align="center" valign="middle" >1</td><td align="center" valign="middle" >First Terrace</td><td align="center" valign="middle" >17.81779</td><td align="center" valign="middle" >33.99229</td><td align="center" valign="middle" >349</td><td align="center" valign="middle" >48.38</td><td align="center" valign="middle" >47.77</td><td align="center" valign="middle" >3.85</td><td align="center" valign="middle" >Sandy Loam</td><td align="center" valign="middle" >5.50</td></tr><tr><td align="center" valign="middle" >2</td><td align="center" valign="middle" >Second Terrace (Farm of Agric. College)</td><td align="center" valign="middle" >17.82389</td><td align="center" valign="middle" >33.99974</td><td align="center" valign="middle" >353</td><td align="center" valign="middle" >86.12</td><td align="center" valign="middle" >12.13</td><td align="center" valign="middle" >1.75</td><td align="center" valign="middle" >Sand</td><td align="center" valign="middle" >2.58</td></tr><tr><td align="center" valign="middle" >3</td><td align="center" valign="middle" >Second Terrace (Instructional Farm)</td><td align="center" valign="middle" >17.82289</td><td align="center" valign="middle" >34.02127</td><td align="center" valign="middle" >358</td><td align="center" valign="middle" >32.61</td><td align="center" valign="middle" >61.14</td><td align="center" valign="middle" >6.25</td><td align="center" valign="middle" >Silt Loam</td><td align="center" valign="middle" >7.40</td></tr></tbody></table></table-wrap><p>with filter paper (Whatman No. 42). Each soil extract then divided into three treatments: i) control (time zero) or immediately analyzed); ii) storage for 10 days and; iii) storage for 30 days. Each treatment was replicated three times: i) storage at 10˚C; ii) storage at ambient laboratory temperature (25˚C) and; iii) storage at 45˚C in incubator.</p></sec><sec id="s2_3"><title>2.3. Estimation of Soil Properties</title><p>The particle size distribution of the subsamples was determined using Particle size analyzer model (Mastersizer 2000, Malvern) and the soil texture class was determined using USDA Textural triangle [<xref ref-type="bibr" rid="scirp.66240-ref10">10</xref>] . Soil pH was measured in 1:5 soil suspensions using a digital pH meter Model (3510, Jenway) and the results were compared according to the classification of [<xref ref-type="bibr" rid="scirp.66240-ref11">11</xref>] . The electrical conductivity (EC dS/m at 25˚C) was determined in 1:5 soil extract using a conductivity meter Model (4510, Jenway) [<xref ref-type="bibr" rid="scirp.66240-ref12">12</xref>] and the results were compared according to the classification of [<xref ref-type="bibr" rid="scirp.66240-ref13">13</xref>] . Percent calcium carbonate (%CaCO<sub>3</sub>) was estimated by Calcimeter. The samples were treated with 0.1N HCL; the volume of CO<sub>2</sub> from pure calcium carbonate and samples were recorded. The percent calcium carbonate was then calculated according to [<xref ref-type="bibr" rid="scirp.66240-ref14">14</xref>] . Extractable cations (Ca<sup>2+</sup>, Mg<sup>2+</sup>, Na<sup>+</sup> and K<sup>+</sup>) and anions (Cl<sup>−</sup>, <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x18.png" xlink:type="simple"/></inline-formula> and<inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x19.png" xlink:type="simple"/></inline-formula>) were determined in 1:5 soil extraction.</p></sec><sec id="s2_4"><title>2.4. Statistical Analysis</title><p>Changes during storage period at 10 and 30 days were determined by repeated measures analysis of variance using storage period and treatment (10˚C, 25˚C and 40˚C) as factors. Values for fresh extracts (control) were compared with those from extracts stored for 10 and 30 days by one-way analysis of variance with Turkey’s significant difference test for mean separation (P &lt; 0.05). All statistical analysis was performed using SPSS software version 16.0 (SPSS Inc, USA).</p></sec></sec><sec id="s3"><title>3. Results and Discussion</title><sec id="s3_1"><title>3.1. Selected Chemical and Physical Properties</title><p>The description of the samples sites in the study area and some of the physical and chemical properties are presented in <xref ref-type="table" rid="table1">Table 1</xref> and <xref ref-type="table" rid="table2">Table 2</xref> respectively. The soil texture ranged from sandy loam in the first terrace, silt loam in the second terrace (Instructional farm). While the soil texture in the Agricultural College farm was sand. This could be due to the fact that these soils falls in the mining area which are truncated soils. The soils of the study area were moderately calcareous and the %CaCO<sub>3</sub> ranged from 2.58% to 7.40%. The soil reaction (Soil pH) varied from alkaline to strongly alkaline with a pH value ranged from 7.46 to 8.80 [<xref ref-type="bibr" rid="scirp.66240-ref11">11</xref>] . According to [<xref ref-type="bibr" rid="scirp.66240-ref13">13</xref>] , the soils of the study area were non-saline and the electrical conductivity (EC<sub>e</sub>) ranged from 0.22 to 0.57 dS/m. The predominant soluble cations and anions in the study area were (Na<sup>+</sup> and Ca<sup>++</sup>), (<inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x20.png" xlink:type="simple"/></inline-formula>and Cl<sup>−</sup>) respectively.</p></sec><sec id="s3_2"><title>3.2. Influence of the Storage Period on the Concentrations of Extractable Nutrients</title><p>Comparing first and second storage period there were significant difference at P &lt; 0.05 found in all estimated cations (<xref ref-type="table" rid="table3">Table 3</xref> and <xref ref-type="fig" rid="fig1">Figure 1</xref>) respectively, except for Na<sup>+</sup> and K<sup>+</sup>. The Ca<sup>2+</sup> and Mg<sup>2+</sup> concentrations were significantly increased from11.22 &#177; 1.978<sup>a</sup> to 17.503 &#177; 4.047<sup>b</sup> and from 3.790 &#177; 2.794<sup>a</sup> - 5.568 &#177; 3.722<sup>b</sup> respectively. Similar trend were found when comparing 10 days with 30 days storage period, Ca<sup>2+</sup> and Mg<sup>2+</sup> concentrations were significantly increased from 17.503 &#177; 4.047<sup>b</sup> to 23.505 &#177; 5.998<sup>c</sup> in addition to K<sup>+</sup> concentration</p><fig id="fig1"  position="float"><label><xref ref-type="fig" rid="fig1">Figure 1</xref></label><caption><title> Change in soluble soil cations concentrations at different storage periods</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/2-2750177x21.png"/></fig><table-wrap id="table2" ><label><xref ref-type="table" rid="table2">Table 2</xref></label><caption><title> Selected soil chemical properties of soils of the study area</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Site No.</th><th align="center" valign="middle"  rowspan="2"  >pH (1:5)</th><th align="center" valign="middle"  rowspan="2"  >EC (dS/m)</th><th align="center" valign="middle"  colspan="5"  >Cations (meq/L)</th><th align="center" valign="middle"  colspan="4"  >Anaions (meq/L)</th></tr></thead><tr><td align="center" valign="middle" >Na<sup>+</sup></td><td align="center" valign="middle" >K<sup>+</sup></td><td align="center" valign="middle" >Mg<sup>++</sup></td><td align="center" valign="middle" >Ca<sup>++</sup></td><td align="center" valign="middle" ><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x22.png" xlink:type="simple"/></inline-formula></td><td align="center" valign="middle" >Cl<sup>−</sup></td><td align="center" valign="middle" ><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x23.png" xlink:type="simple"/></inline-formula></td><td align="center" valign="middle" ><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x24.png" xlink:type="simple"/></inline-formula></td><td align="center" valign="middle" ><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x25.png" xlink:type="simple"/></inline-formula></td></tr><tr><td align="center" valign="middle" >1</td><td align="center" valign="middle" >8.73</td><td align="center" valign="middle" >0.219</td><td align="center" valign="middle" >1.699</td><td align="center" valign="middle" >0.547</td><td align="center" valign="middle" >0.196</td><td align="center" valign="middle" >0.558</td><td align="center" valign="middle" >0.050</td><td align="center" valign="middle" >0.538</td><td align="center" valign="middle" >0.090</td><td align="center" valign="middle" >0.290</td><td align="center" valign="middle" >2.193</td></tr><tr><td align="center" valign="middle" >2</td><td align="center" valign="middle" >8.46</td><td align="center" valign="middle" >0.250</td><td align="center" valign="middle" >1.106</td><td align="center" valign="middle" >0.281</td><td align="center" valign="middle" >0.503</td><td align="center" valign="middle" >0.640</td><td align="center" valign="middle" >0.063</td><td align="center" valign="middle" >0.575</td><td align="center" valign="middle" >0.460</td><td align="center" valign="middle" >0.186</td><td align="center" valign="middle" >1.377</td></tr><tr><td align="center" valign="middle" >3</td><td align="center" valign="middle" >8.80</td><td align="center" valign="middle" >0.570</td><td align="center" valign="middle" >2.811</td><td align="center" valign="middle" >0.248</td><td align="center" valign="middle" >0.172</td><td align="center" valign="middle" >0.352</td><td align="center" valign="middle" >0.057</td><td align="center" valign="middle" >0.522</td><td align="center" valign="middle" >0.081</td><td align="center" valign="middle" >0.643</td><td align="center" valign="middle" >2.424</td></tr></tbody></table></table-wrap><table-wrap id="table3" ><label><xref ref-type="table" rid="table3">Table 3</xref></label><caption><title> Influence of storage period on the concentrations of soluble soil cations</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Cations</th><th align="center" valign="middle"  colspan="3"  >Treatments (storage period)</th></tr></thead><tr><td align="center" valign="middle" >Time zero</td><td align="center" valign="middle" >10 days</td><td align="center" valign="middle" >30 days</td></tr><tr><td align="center" valign="middle" >Na<sup>+</sup></td><td align="center" valign="middle" >3.984 &#177; 5.675<sup>a</sup></td><td align="center" valign="middle" >3.881 &#177; 5.6636<sup>a</sup></td><td align="center" valign="middle" >3.943 &#177; 5.733<sup>a</sup></td></tr><tr><td align="center" valign="middle" >K<sup>+</sup></td><td align="center" valign="middle" >3.279 &#177; 1.243<sup>a</sup></td><td align="center" valign="middle" >3.582 &#177; 1.306<sup>a</sup></td><td align="center" valign="middle" >3.968 &#177; 1.430<sup>b</sup></td></tr><tr><td align="center" valign="middle" >Mg<sup>2+</sup></td><td align="center" valign="middle" >3.790 &#177; 2.794<sup>a</sup></td><td align="center" valign="middle" >5.568 &#177; 3.722<sup>b</sup></td><td align="center" valign="middle" >7.334 &#177; 4.739<sup>c</sup></td></tr><tr><td align="center" valign="middle" >Ca<sup>2+</sup></td><td align="center" valign="middle" >11.22 &#177; 1.978<sup>a</sup></td><td align="center" valign="middle" >17.503 &#177; 4.047<sup>b</sup></td><td align="center" valign="middle" >23.505 &#177; 5.998<sup>c</sup></td></tr></tbody></table></table-wrap><p>Means in each row with different letters are significantly different at P &lt; 0.05.</p><p>which increased from 3.582 &#177; 1.306<sup>a</sup> to 3.968 &#177; 1.430<sup>b</sup>. These obtained results were expected specially increasing the storage period logically content in soluble solution. When we compared first and third storage period there were significant difference found in K<sup>+</sup> and highly significant difference observed in Mg<sup>2+</sup> (from 3.790 &#177; 2.794<sup>a</sup> to 7.334 &#177; 4.739<sup>c</sup>) and Ca<sup>++</sup> (from 11.22 &#177; 1.978<sup>a</sup> to 23.505 &#177; 5.998<sup>c</sup>) at P &lt; 0.05 level. [<xref ref-type="bibr" rid="scirp.66240-ref15">15</xref>] reported that extractable Ca<sup>2+</sup>, K<sup>+</sup>, and Mg<sup>2+</sup> were less influenced by storage period, although the effects varied among soil types.</p><p>In anions part (<xref ref-type="table" rid="table4">Table 4</xref> and <xref ref-type="fig" rid="fig2">Figure 2</xref>) respectively, while comparing first and second period results shown that Cl<sup>−</sup> and <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x26.png" xlink:type="simple"/></inline-formula> concentrations were significantly increased from0.318 &#177; 3.553<sup>a</sup> to 0.749 &#177; 1.498<sup>b</sup> and 0.808 &#177; 3.550<sup>a</sup> to 0.885 &#177; 5.710<sup>b</sup> respectively. However, <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x26.png" xlink:type="simple"/></inline-formula><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x27.png" xlink:type="simple"/></inline-formula>concentrations<sup> </sup>were highly significant decreased (from 12.67 &#177; 2.158<sup>a</sup> to 3.577 &#177; 3.186<sup>c</sup>) with increased of storage period. Similar results were obtained by [<xref ref-type="bibr" rid="scirp.66240-ref15">15</xref>] . Contrasting, [<xref ref-type="bibr" rid="scirp.66240-ref16">16</xref>] reported that soil storage before separation of the soil solution by centrifugation caused a marked decline in P concentrations.</p></sec><sec id="s3_3"><title>3.3. Influence of Temperatures on the Concentrations of Extractable Nutrients</title><p>Comparing first and second temperature (10˚C and 25˚C), significant different were found in Mg<sup>2+</sup> and Ca<sup>2+</sup></p><fig id="fig2"  position="float"><label><xref ref-type="fig" rid="fig2">Figure 2</xref></label><caption><title> Change in soluble soil anions concentrations at different storage periods</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/2-2750177x28.png"/></fig><fig id="fig3"  position="float"><label><xref ref-type="fig" rid="fig3">Figure 3</xref></label><caption><title> Change in soluble anions concentrations at different temperatures</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/2-2750177x29.png"/></fig><table-wrap id="table4" ><label><xref ref-type="table" rid="table4">Table 4</xref></label><caption><title> Influence of storage period on the concentrations of soluble soil anions</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Anions</th><th align="center" valign="middle"  colspan="3"  >Treatments (storage period)</th></tr></thead><tr><td align="center" valign="middle" >Time zero</td><td align="center" valign="middle" >10 days</td><td align="center" valign="middle" >30 days</td></tr><tr><td align="center" valign="middle" >Cl<sup>−</sup></td><td align="center" valign="middle" >0.318 &#177; 3.553<sup>a</sup></td><td align="center" valign="middle" >0.749 &#177; 1.498<sup>b</sup></td><td align="center" valign="middle" >0.664 &#177; 1.631<sup>b</sup></td></tr><tr><td align="center" valign="middle" ><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x30.png" xlink:type="simple"/></inline-formula></td><td align="center" valign="middle" >12.67 &#177; 2.158<sup>a</sup></td><td align="center" valign="middle" >12.063 &#177; 2.120<sup>a</sup></td><td align="center" valign="middle" >3.577 &#177; 3.186<sup>c</sup></td></tr><tr><td align="center" valign="middle" ><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x31.png" xlink:type="simple"/></inline-formula></td><td align="center" valign="middle" >0.808 &#177; 3.550<sup>a</sup></td><td align="center" valign="middle" >0.885 &#177; 5.710<sup>b</sup></td><td align="center" valign="middle" >1.143 &#177; 5.586<sup>b</sup></td></tr></tbody></table></table-wrap><p>*Means in each row with different letters are significantly different at P &lt; 0.05.</p><p>from 3.790 &#177; 1.452<sup>a</sup> to 5.568 &#177; 1.934<sup>b</sup> and 11.22 &#177; 1.028<sup>a</sup> to 17.503 &#177; 2.103<sup>b</sup> respectively. Similarly significant different are found while comparing K<sup>+</sup> in second and third temperature (25˚C and 45˚C), and highly significant different were found while comparing second and third temperature in Mg<sup>2+</sup> and Ca<sup>2+</sup> respectively (<xref ref-type="table" rid="table5">Table 5</xref> and <xref ref-type="fig" rid="fig3">Figure 3</xref>). Similar results were obtained by [<xref ref-type="bibr" rid="scirp.66240-ref15">15</xref>] . Additionally, change in K<sup>+</sup> and Ca<sup>2+</sup> concentrations during low temperature storage were observed to be less significant and associated with soil types [<xref ref-type="bibr" rid="scirp.66240-ref17">17</xref>] .</p><p>Comparing first and second temperature (10˚C and 25˚C), significant different were found in Cl<sup>−</sup> and <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x32.png" xlink:type="simple"/></inline-formula><sub> </sub>from 0.318 &#177; 0.185<sup>a</sup> to 0.749 &#177; 0.779<sup>b</sup> and 0.808 &#177; 1.844<sup>a</sup> to 0.885 &#177; 2.967<sup>b</sup> respectively. [<xref ref-type="bibr" rid="scirp.66240-ref14">14</xref>] , in their study reported that, extractable PO<sub>4</sub> of Refrigerated samples always contained lower values than counterparts stored at</p><p>room temperature. They also pointed out that, extractable <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x33.png" xlink:type="simple"/></inline-formula> were significantly differences among the soil types. Moreover, the amounts of extractable <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x33.png" xlink:type="simple"/></inline-formula><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x34.png" xlink:type="simple"/></inline-formula> were significantly increased during low temperature storage [<xref ref-type="bibr" rid="scirp.66240-ref15">15</xref>] . In contrast, [<xref ref-type="bibr" rid="scirp.66240-ref17">17</xref>] , reported that soil storage before separation of the soil solution by centrifugation caused a marked decline in P concentrations, including those of molybdate-reactive P and dissolved organic P, within a few days. However, <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x33.png" xlink:type="simple"/></inline-formula><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x34.png" xlink:type="simple"/></inline-formula><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x35.png" xlink:type="simple"/></inline-formula>concentrations were significantly influenced by increased temperature (<xref ref-type="table" rid="table6">Table 6</xref> and <xref ref-type="fig" rid="fig4">Figure 4</xref>).</p></sec></sec><sec id="s4"><title>4. Conclusion</title><p>Depending on our results, all investigated cations and anions were clearly affected by storage periods at various temperatures, exception of Na<sup>+</sup>. Most cations and anions were increased significantly with increased of storage period and temperatures, while some anions (such as <inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x36.png" xlink:type="simple"/></inline-formula> and Cl<sup>−</sup>) were dramatically decreased with increased of storage period and temperatures. Despite the simplicity of this research and some limitations such as design which was performed, but the obtained results confirmed that the storage periods and temperatures were influenced markedly on the concentrations of different extractable inorganic soil nutrients. We therefore highly</p><fig id="fig4"  position="float"><label><xref ref-type="fig" rid="fig4">Figure 4</xref></label><caption><title> Change in soluble anions concentrations at different temperatures</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/2-2750177x37.png"/></fig><table-wrap id="table5" ><label><xref ref-type="table" rid="table5">Table 5</xref></label><caption><title> Influence of storage period on the concentrations of soluble soil anions</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Cations</th><th align="center" valign="middle"  colspan="3"  >Treatments (storage period)</th></tr></thead><tr><td align="center" valign="middle" >10˚C</td><td align="center" valign="middle" >25˚C</td><td align="center" valign="middle" >45˚C</td></tr><tr><td align="center" valign="middle" >Na<sup>+</sup></td><td align="center" valign="middle" >3.984 &#177; 29.49<sup>a</sup></td><td align="center" valign="middle" >3.881 &#177; 29.426<sup>a</sup></td><td align="center" valign="middle" >3.943 &#177; 29.794<sup>a</sup></td></tr><tr><td align="center" valign="middle" >K<sup>+</sup></td><td align="center" valign="middle" >3.279 &#177; 0.645<sup>a</sup></td><td align="center" valign="middle" >3.582 &#177; 0.679<sup>a</sup></td><td align="center" valign="middle" >3.968 &#177; 0.743<sup>b</sup></td></tr><tr><td align="center" valign="middle" >Mg<sup>++</sup></td><td align="center" valign="middle" >3.790 &#177; 1.452<sup>a</sup></td><td align="center" valign="middle" >5.568 &#177; 1.934<sup>b</sup></td><td align="center" valign="middle" >7.334 &#177; 2.463<sup>c</sup></td></tr><tr><td align="center" valign="middle" >Ca<sup>++</sup></td><td align="center" valign="middle" >11.22 &#177; 1.028<sup>a</sup></td><td align="center" valign="middle" >17.503 &#177; 2.103<sup>b</sup></td><td align="center" valign="middle" >23.505 &#177; 3.117<sup>c</sup></td></tr></tbody></table></table-wrap><p><sup>*</sup>Means in each row with different letters are significantly different at P &lt; 0.05.</p><table-wrap id="table6" ><label><xref ref-type="table" rid="table6">Table 6</xref></label><caption><title> Influence of temperature on different concentrations of soluble anions</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Anions</th><th align="center" valign="middle"  colspan="3"  >Treatments (temperature)</th></tr></thead><tr><td align="center" valign="middle" >10˚C</td><td align="center" valign="middle" >25˚C</td><td align="center" valign="middle" >45˚C</td></tr><tr><td align="center" valign="middle" >Cl<sup>−</sup></td><td align="center" valign="middle" >0.318 &#177; 0.185<sup>a</sup></td><td align="center" valign="middle" >0.749 &#177; 0.779<sup>b</sup></td><td align="center" valign="middle" >0.664 &#177; 0.848<sup>b</sup></td></tr><tr><td align="center" valign="middle" ><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x38.png" xlink:type="simple"/></inline-formula></td><td align="center" valign="middle" >12.67 &#177; 11.21<sup>a</sup></td><td align="center" valign="middle" >12.063 &#177; 11.02<sup>a</sup></td><td align="center" valign="middle" >3.577 &#177; 1.314<sup>c</sup></td></tr><tr><td align="center" valign="middle" ><inline-formula><inline-graphic xlink:href="http://html.scirp.org/file/2-2750177x39.png" xlink:type="simple"/></inline-formula></td><td align="center" valign="middle" >0.808 &#177; 1.844<sup>a</sup></td><td align="center" valign="middle" >0.885 &#177; 2.967<sup>b</sup></td><td align="center" valign="middle" >1.143 &#177; 2.902<sup>b</sup></td></tr></tbody></table></table-wrap><p><sup>*</sup>Means in each row with different letters are significantly different at P &lt; 0.05.</p><p><sup>*</sup>Corresponding author.</p></sec><sec id="s5"><title>Acknowledgements</title><p>The authors are greatly indebted to dean of Agriculture College and general manager of Educational Farm, Nile Valley University.</p></sec><sec id="s6"><title>Cite this paper</title><p>Jamal T. Elfaki,Ahmed M. Nour,Magboul M. Sulieman,Mushtaha E. Ali, (2016) Influence of the Storage Duration at Different Temperatures on the Concentrations of Extractable Inorganic Soil Nutrients. Journal of Agricultural Chemistry and Environment,05,66-72. doi: 10.4236/jacen.2016.52007</p></sec><sec id="s7"><title>NOTES</title></sec></body><back><ref-list><title>References</title><ref id="scirp.66240-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">Boczulak, S.A., Hawkins, B.J. and Roy, R. (2014) Temperature Effects on Nitrogen Form Uptake by Seedling Roots of Three Contrasting Conifers. 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