IJOCInternational Journal of Organic Chemistry2161-4687Scientific Research Publishing10.4236/ijoc.2016.61007IJOC-65203ArticlesBiomedical&Life Sciences Chemistry&Materials Science Synthesis, Reactions and Characterization of 1,1’-(1,4-Phenylenebis(3-amino-6-methyl-1H-pyrazolo[3,4-b]pyridine-4,5-diyl))bis(ethan-1-one) hmedA. M. Elreedy1HameedM. Alkubaisi2FawzyA. Attaby3*Chemistry Department, Faculty of Science, Cairo University, Giza, EgyptCollege of Science for Women, Baghdad University, Baghdad, IraqBasic-Applied Science Department, Faculty of Oral and Dental Medicine, Nahda University, Beni-Suef, Egypt* E-mail:fattaby@gmail.com(FAA);040320160601657615 January 2016accepted 27 March 30 March 2016© Copyright 2014 by authors and Scientific Research Publishing Inc. 2014This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/

Reaction of 4,4’-(1,4-phenylene)bis(5-acetyl-6-methyl-2-thioxo-1,2-dihydropyridine-3-carbonitrile) (1) with methyl iodide afforded the 4,4’-(1,4-phenylene)bis(5-acetyl-6-methyl-2-(methylthio)nicotinonitrile) (2). The reaction of 2 with hydrazine hydrate followed by diazotization reaction af-forded the 1,1’-(1,4-phenylenebis(3-amino-6-methyl-1H-pyrazolo[3,4-b]pyridine-4,5-diyl))bis(e-than-1-one) (3) and 1,1’-(1,4-phenylenebis(3-(chlorodiazenyl)-6-methyl-1H-pyrazolo[3,4-b]-pyridine-4,5-diyl))bis(ethan-1-one) (4) respectively. On the other hand, reaction of 4 with malononitrile, 2-cyanoethanethioamide, ethyl acetoacetate, acetyl acetone, ethyl benzoylacetate, diethylmalonate, ethyl cyanoacetate and phenacylbromide aiming to build up pyrazolotriazine or pyrazole ring on the ring system of 4. Structures of all newly synthesized heterocyclic compounds in the present study were confirmed by considering the data of IR, 1H NMR, mass spectra as well as that of elemental analyses.

Bis-12-dihydropyridine-3-carbonitrile Bis-Nicotinonitrile 11’-(14-Phenyl-enebis(3-(chlorodiazenyl)-6-methyl-1H-pyrazolo[34-b]pyri-dine-45-diyl))bis(ethan-1-one) Bis-dihydropyrido[2’3’:34]pyrazolo[51-c][124]triazine-3-carboxylate Bis-1H-pyrazolo[34-b]pyridine1. Introduction

The chemistry of heterocyclic compounds has attracted attention in recent times due to its increasing importance in the field of pharmaceuticals and industries. In fact, the synthesis of bis-heterocyclic compounds exhibits various biological activities [1] - [8] including antibacterial, fungicidal, tuberculostatic, antiamoebic, and plant growth regulative properties. Also, bis-compounds have received great attention [9] [10] not only for being model compounds for main chain polymers [11] - [16] but also because many biologically active natural and synthetic products have molecular symmetry [17] . The biological importance of both bis-compounds [18] - [21] and 2-thioxopyridine-3-carbonitriles [22] - [24] as well as the conjunction to our previous work [25] - [33] stimulated our interest to synthesize several derivatives of these ring systems that are required for several chemical transformations and medicinal chemistry programs.

2. Experimental

All melting points were uncorrected. I. R. (KBrdiscs) spectra were recorded on a Shimadzu FTIR-8201PC Spectrophotometer. 1HNMR spectra were recorded on a Varian Mercury 300 MHz., and a Varian Gemini 200 MHz. Spectrometers using TMS as an internal standard and CDCl3, DMSO-d6, and (CD3)2CO as solvents. Chemical shifts were expressed as δ(ppm) units. Mass spectra were recorded on Agilent LC 1200/MS Ion Trap 6320 using APCI ionization source and the spectra was enhanced using acidified water/acetonitrile mobile phase and measured in the positive mode of the ion trap (Molecular weights of most compounds were protonated ([M+H]+).

2.1. Synthesis of 2

Methyl iodide (3.6 g, 50 mmole) was added to a solution of 1 (22.9 g, 50 mmole) in methanol (100 mL) containing potassium hydroxide (2.8 g, 50 mmole) with stirring. The reaction mixture stir for 1 h, then the solid filtered and recrystallized from the dioxane to afford 2.

4,4’-(1,4-Phenylene)bis(5-acetyl-6-methyl-2-(methylthio)nicotinonitrile) (2): as orange crystals, yield 56%, mp > 330˚C, IR (ν∙cm−1): 3164.1 (Pyridine-NH), 3058.8 (aromatic-CH) ), 2948.2, 2855.1 (aliphatic-CH), 2228.4 (CN), and 1694.2 (Acetyl-CO); 1HNMR (dppm): 2.43 (s, 6H, two SCH3), 2.53 (s, 6H, two CH3), 2.78 (s, 6H, two CH3CO), 7.1 - 7.57 (m, 4H, phenyl H’s) MS: 487 ([M+H]+, 1.5%, corresponding to the molecular weight of the molecular formula C26H22N4O2S2 of the assigned structure), 471 (486-CH3, 12.6%), 456 (486-2CH3, 10.9%), 439 (486-SCH3, 23.5%), 392 (486-2SCH3, 5.7%), 349 (486-2SCH3, COCH3, 0.5%), 306 (486-2SCH3, 2COCH3, 100%); Anal. for C26H22N4O2S2 (486.6) Calcd./Found (%): C (64.17/64.19%) H (4.56/4.59%) N (11.51/11.55%) S (13.18/13.21%).

2.2. Synthesis of 3

A solution of 2 (4.86 g, 10 mmole) in hydrazine hydrate (10 ml), ethanol (20 mL) and pyridine (10 mL) was heated under reflux until the odor of methyl mercaptan ceased. The excess solvent was evaporated, and the solids so formed after cooling were collected by filtration, and recrystallized from ethanol to give the corresponding 3.

1,1’-(1,4-Phenylenebis(3-amino-6-methyl-1H-pyrazolo[3,4-b]pyrid-ine-4,5-diyl))bis(ethan-1-one) (3): as yellow crystals, yield 52%, mp 222˚C, IR (ν∙cm−1): 3385, 3333, 3185 (NH2, NH), 3055 (aromatic-CH), 2956, 2876 (aliphatic-CH) and 1697 (Acetyl-CO); 1HNMR (dppm): 2.52 (s, 6H, two CH3), 2.78 (s, 6H, two CH3CO), 4.3 (s, br., 4H, two NH2), 7.0 - 7.55 (m, 4H, phenyl H’s); 12.3 (s, br., 2H, two NH); MS: 455 ([M+H]+, 2.5%, corresponding to the molecular weight of the molecular formula C24H22N8O2 of the assigned structure), 439 (454-CH3, 11.2%), 424 (454-2CH3, 18.1%), 411 (454-COCH3, 2.6%), 368 (454-2COCH3, 100%); Anal. for C24H22N8O2 (454) Calcd./Found (%): C (63.43/63.40%) H (4.88/4.89%) N (24.66/24.65%).

2.3. Synthesis of 4

A solution of 3 (4.54 g, 10 mmole) in concentrated hydrochloric acid (10 mL) and glacial acetic acid (20 mL) was stirred in an ice bath. Sodium nitrite solution was added (4 g, dissolved in 20 ml cold water) drop wise with stirring for 50 minutes. The solids so formed were collected by filtration, washed with water and dried to give the corresponding 4.

1,1’-(1,4-Phenylenebis(3-(chlorodiazenyl)-6-methyl-1H-pyrazolo[3,4-b]-pyridine-4,5-diyl))bis(ethan-1-one) (4):as pale yellow crystals, yield 44%, mp 98˚C, IR (ν∙cm−1): 3184 (NH), 3066 (aromatic-CH) ), 2952, 2878 (aliphatic-CH) and 1687 (Acetyl-CO); 1HNMR (dppm): 2.42(s, 6H, two CH3), 2.82 (s, 6H, two CH3CO), 7.1 - 7.78 (m, 4H, phenyl H’s); 11.7 (s, br., 2H, two NH); MS: 550 ([M+H]+, 2.5%, corresponding to the molecular weight of the molecular formula C24H18Cl2N10O2 of the assigned structure), 534 (549-CH3, 9.2%), 519 (549- 2CH3, 22.3%), 506 (549-COCH3, 4.1%), 463 (549-2COCH3, 34.2%), 427 (549-twoN2Cl, 100%); Anal. for C24H18Cl2N10O2 (549) Calcd./Found (%): C (52.47/52.50) H (3.30/3.33) N (25.50/25.53), Cl 12.91/12.89

2.4. Synthesis of 5, 6, 7, 8, 9, 10 and 12

A solution of 4 (1.64 g, 3 mmole) in ethanol (30 mL) containing 2.0 g sodium acetate and malononitrile, cyanothioacetamide, ethyl acetoacetate, acetylacetone, ethyl benzoylacetate, diethylmalonate and ethyl cyano-acetate (0.30 g, 0.25 g, 0.38 g, 0.30 g, 0.57 g, 0.48 g and 0.33 g 3 mmole) was stirred at room temperature for one hour. The reaction mixture was then poured onto ice-cold water formed precipitate were collected by filtration, washed with water and recrystallized from the proper solvent to afford 5, 6, 7, 8, 9, 10 and 12 respectively.

10,10’-(1,4-Phenylene)bis(9-acetyl-4-amino-8-methyl-6,10b-dihydro-pyrido[2’,3’-:3,4]pyrazolo[5,1-c][1,2,4]triazine-3-carbonitrile) (5): as pale yellow crystals, yield 44%, mp > 301˚C, IR (ν∙cm−1): 3288, 3279, 3184 (NH2, NH), 3066 (aromatic-CH) ), 2952, 2878 (aliphatic-CH), 2218 (CN) and 1687 (Acetyl-CO); 1HNMR (dppm): 2.42 (s, 6H, two CH3), 2.82 (s, 6H, two CH3CO), 2.91 (s, br., 4H, 2NH2), 7.1 - 7.78 (m, 4H, phenyl H’s); 11.7 (s, br., 2H, two NH); MS: 613 ([M+H]+, 2.5%, corresponding to the molecular weight of the molecular formula C30H24N14O2 of the assigned structure), 611 (612-H, 65.3%), 610 (612-2H, 23.1%), 599 (612-CH3, 19.5%), 584 (612-2CH3, 15.8%), 569 (612-COCH3, 14.7%), 526 (612-2COCH3, 23.5%); Anal. for C30H24N14O2 (612.6) Calcd./Found (%): C (58.82/58.85) H (3.95/3.93) N (32.01/32.03).

10,10’-(1,4-Phenylene)bis(9-acetyl-4-amino-8-methyl-6,10b-dihydro-pyrido[2’,3’-:3,4]pyrazolo[5,1-c][1,2,4]triazine-3-carbothioamide) (6): as yellow crystals, yield 44%, mp > 300˚C, IR (ν∙cm−1): 3288, 3279, 3184 (NH2, NH), 3061 (aromatic-CH) ), 2959, 2879 (aliphatic-CH) and 1679 (Acetyl-CO); 1HNMR (dppm): 2.23 (s, 6H, two CH3), 2.54 (s, 6H, two CH3CO), 2.59 (s, br., 8H, four NH2), 3.6 (s, 2H, triazine H-3), 4.7(s, br., 2H, two NH), 7.1 - 7.78 (m, 4H, phenyl H’s); MS: 613 ([M+H]+, 2.5%, corresponding to the molecular weight of the molecular formula C30H28N14O2S2 of the assigned structure), 611 (612-H, 65.3%), 610 (612-2H, 23.1%), 599 (612-CH3, 19.5%), 584 (612-2CH3, 15.8%), 569 (612-COCH3, 14.7%), 526 (612-2COCH3, 23.5%); Anal. for C30H24N14O2 (612.6) Calcd./Found (%): C (58.82/58.85%) H (3.95/3.93%) N (32.01/32.03%).

10,10’-(1,4-Phenylene)bis(9-acetyl-4-amino-8-methyl-6,10b-dihydropyrido[2’,3’:3,4]-pyrazolo[5,1-c][1,2, 4]triazine-3-carbothioamide) (7): as yellow crystals, yield 44%, mp 249˚C, IR (ν∙cm−1): 3184 (NH), 3079 (aromatic-CH)), 2978, 2859 (aliphatic-CH) and 1719 (CO ester), 1689 (Acetyl-CO); 1HNMR (dppm):1.21 (t, 6H, two CH3CH2-), 1.7 (s, 6H, two CH3), 2.35 (s, 6H, two CH3), 2.63 (s, 6H, two CH3CO), 3.5 (s, 2H, triazine H-3), 4.3 (q, 4H, two CH3CH2-), 5.4 (s, br., 2H, two NH), 7.2 - 7.88 (m, 4H, phenyl H’s); MS: 705 ([M+H]+, 4.1%, corresponding to the molecular weight of the molecular formula C36H36N10O6 of the assigned structure), 611 (612-H, 65.3%), 610 (612-2H, 23.1%), 599( 612-CH3, 19.5%), 584 (612-2CH3, 15.8%), 569 (612-COCH3, 14.7%), 526 (612-2COCH3, 23.5%); Anal. for C36H36N10O6 (704.7) Calcd./Found (%): C (61.35/61.35%) H (5.15/5.13%) N (19.88/19.87%).

1,1’-(10,10’-(1,4-Phenylene)bis(4,9-dimethyl)-6,10b-dihydropyrido[2’,3’:3,4]pyrazo-lo[5,1-c][1,2,-4]triazine-10,9-diyl)-diethanone (8): as yellow crystals, yield 44%, mp > 330˚C, IR (ν∙cm−1): 3184 (NH), 3073(aro- matic-CH)), 2974, 2851 (aliphatic-CH) and 1689 (Acetyl-CO); 1HNMR (dppm): 1.3 (s, 6H, two CH3 at triazine), 2.11 (s, 6H, 2CH3 at pyridine), 2.58 (s, 6H, two CH3CO at triazine), 2.69 (s, 6H, two CH3CO at pyridine), 3.8 (s, 2H, triazine H-3), 5.1 (s, br., 2H, two NH), 7.1 - 8.01 (m, 4H, phenyl H’s); MS: 645 ([M+H]+, 3.5%, corresponding to the molecular weight of the molecular formula C34H32N10O4 of the assigned structure), 644 (643-H, 15.1%), 642 (644-2H, 12.3%), 629 (644-CH3, 22.3%), 601 (644-COCH3, 31.6%), 558 (644-2COCH3, 100%); Anal. for C36H36N10O6 (644.7) Calcd./Found (%): C (63.34/63.35%) H (5.00/5.03%) N (21.73/21.77%).

1,1’-(10,10’-(1,4-Phenylene)bis(3-benzoyl-4-hydroxy-8-methyl-6,10b-dihydropyrido-[2’,3’:3,4]pyrazolo[5,1-c][1,2,4]triazine-10,9-diyl))dieth-anone (9): as yellow crystals, yield 44%, mp > 312˚C, IR (ν∙cm−1): 2559 - 3321 (broad band OH), 3058 (aromatic-CH)), 2979, 2889 (aliphatic-CH) and 1689 (Benzoyl-CO); 1HNMR (dppm): 1.8 (s, 6H, two CH3 at pyridine), 2.65 (s, 6H, two CH3CO at pyridine), 3.8 (s, 2H, triazine H-3), 4.4 (s, br., 2H, two NH), 7.3 - 8.4 (m, 14H, phenyl H’s), 11.3 (s, br., 2H, two OH); MS: 773 ([M+H]+, 0.5%, corresponding to the molecular weight of the molecular formula C42H32N10O6 of the assigned structure), 771 (643-H, 15.1%), 770 (772-2H, 9.1%), 757 (772-CH3, 12.5%), 729 (772-COCH3, 42.1%), 686 (772-2COCH3, 11.0%), 667 (772-PhCO, 51.1%), 562 (772-2PhCO, 62.2%), 476 (772-2PhCO, 2CH3CO, 100%); Anal. for C42H32N10O6 (772.7) Calcd./Found (%): C (65.28/65.30%) H (4.17/4.20%) N (18.13/18.17%).

Tetraethyl 2,2’-(2,2’-(4,4’-(1,4-phenylene)bis(5-acetyl-6-methyl-2,3-di-hydro-1H-pyrazolo[3,4-b]pyridine-4, 3-diyl))bis(hydrazin-2-yl-1-ylidene))dimalonate (10): as yellow crystals, yield 44%, mp 243˚C, IR (ν∙cm−1): 3188, 3166 (NH), 2877-2965 (C-H aliphatic), 3072 (aromatic-CH)), 1723 (Ester-CO) and 1689 (CO-Acetyl); 1HNMR (dppm): 1.09 (t, 8H, 4CH3CH2), 1.5 (s, 6H, two CH3 at pyridine), 2.34 (q, 12H, 4CH3CH2), 2.62 (s, 6H, 2CH3CO), 3.9 (s, 2H, triazine H-3), 4.1 (s, br., 4H, 2NH), 6.8 (s, br., 2H, 2NH), 7.2 - 8.1 (m, 4H, phenyl H’s); MS: 801 ([M+H]+, 3.1%, corresponding to the molecular weight of the molecular formula C38H44N10O10 of the assigned structure), 799 (800-H, 4.6%), 798 (800-2H, 12.4%), 785 (800-CH3, 22.1%), 757 (800-COCH3, 14.5%), 714 (800-2COCH3, 32.1%), 710 (800-2EtO, 5.3%), 620 (800-2EtO, 12.5%), 508 (800-4COOEt, 30.4%), 456 (800-2 N=C (COOEt)2, 100%); Anal. for C38H44N10O10 (800.8) Calcd./Found (%): C (56.99/57.00%) H (5.54/ 5.53%) N (17.49/17.50%).

Diethyl 2,2’-(2,2’-(4,4'-(1,4-phenylene)bis(5-acetyl-6-methyl-2,3-di-hydro-1H-pyrazolo[3,4-b]pyridine-4, 3-diyl))bis(hydrazin-2-yl-1-ylidene))bis(3-cyanopropano-ate) (12): as yellow crystals, yield 44%, mp > 300˚C, IR (ν∙cm−1): 3180, 3166 (NH), 2879 - 2966 (C-H aliphatic), 3078 (aromatic-CH)), 2219 (CN), 1722 (Ester-CO) and 1688 (CO-Acetyl); 1HNMR (dppm): 1.11 (t, 4H, 2CH3CH2), 1.62 (s, 6H, two CH3 at pyridine), 2.1 (s, br., 2H, 2NH-pyrazolidine), 2.45 (s, 6H, 2CH3CO), 2.61 (s, 4H, 2CH2CN), 3.7(s, 2H, triazine H-3), 4.4(s, br., 2H, 2NH-pyrazolidine), 4.69 (q, 6H, 2CH3CH2), 6.8 (s, br., 2H, 2NH-hydrazidic), 7.0 - 8.3 (m, 4H, phenyl H’s); MS:735 ([M+H]+, 1.9%, corresponding to the molecular weight of the molecular formula C36H38N12O6 of the assigned structure), 733 (734-H, 1.6%), 732 (734-2H, 2.4%), 718 (734-CH3, 12.3%), 690 (734-COCH3, 11.9%), 647 (734-2COCH3, 32.5%), 603(734-EtO,2COCH3, 17.8%), 575 (734-EtOOC, 2COCH3, 32.1%), 502 (734 - 2COOEt, 2COCH3, 63.5%), 480 (734-2COOEt, 2CH2CN, 2N, 100%); Anal. for C36H38N12O6 (734.7) Calcd./ Found (%): C (58.85/58.90%) H (5.21/5.23%) N (22.88/22.90%).

2.5. Synthesis of 11 and 13

A solution of 4 (1.64 g, 3 mmole) in ethanol (30 ml) diethylmalonate and ethyl cyanoacetate (0.48 g and 0.33 g 3 mmole) in presence of piperidine drops, was heated under reflux for 4 hours. The excess solvent was evaporated. The reaction mixture was then cooled and the solids were collected by filtration, dried, and crystallized to give 11 and 13 respectively.

Diethyl 10,10’-(1,4-phenylene)bis(9-acetyl-4-hydroxy-8-methyl-6,10b-dihydropy-rido[2’,3’:3,4]pyrazolo [5,1-c][1,2,4]triazine-3-carboxylate) (11): as yellow crystals, yield 44%, mp 300˚C, IR (ν∙cm−1): 2566 - 3288 (broad band, OH), 2879 - 2982 (C-H aliphatic), 3079 (aromatic-CH)), 1722 (Ester-CO) and 1679 (CO-Acetyl); 1HNMR (dppm): 1.31 (t, 6H, 2CH3CH2), 1.82 (s, 6H, two CH3 at pyridine), 2.67 (s, 6H, 2CH3CO), 3.62 (s, 2H, triazine H-3), 4.1 (s, br., 2H, 2NH), 4.51 (q, 4H, 2CH2CH3), 7.2 - 8.1 (m, 4H, phenyl H’s), 13.4 (s, br., 2H, 2OH); MS:709 ([M+H]+, 6.0%, corresponding to the molecular weight of the molecular formula C34H32N10O8 of the assigned structure), 691 (708-OH, 12.3%), 674 (708-2OH, 24.7%), 663 (708-OCH2CH3, 7.9%), 635 (708- EtOOC, 23.8%), 590 (708-EtO, COOEt, 7.1%), 562 (708-4COOEt, 42.1%), 519 (708-2(COOEt)2, COCH3, 61.3%), 476 (800-2(COOEt)2, 2COCH3, 100%); Anal. for C34H32N10O8 (708.7) Calcd./Found (%): C (57.62/ 57.70%) H (4.55/4.53%) N (19.76/19.72%).

Diethyl 10,10’-(1,4-phenylene)bis(9-acetyl-4-amino-8-methyl-6,10b-dihydropyr-ido[2’,3’:3,4]pyrazolo [5,1- c][1,2,4]triazine-3-carboxylate) (13): as yellow crystals, yield 44%, mp 243˚C, IR (ν∙cm−1): 3358, 3256, 3187 (NH2, NH), 2877 - 2989 (C-H aliphatic), 3082 (aromatic-CH)), 1721 (Ester-CO) and 1679 (CO-Acetyl); 1HNMR (dppm): 1.30 (t, 6H, 2CH3CH2), 1.87 (s, 6H, two CH3 at pyridine), 2.68 (s, 6H, 2CH3CO), 2.91 (s, br., 4H, 2NH2), 3.87 (s, 2H, triazine H-3), 4.1 (s, br., 2H, 2NH), 4.51 (q, 4H, 2CH2CH3), 7.2 - 8.1 (m, 4H, phenyl H's); MS: 709 ([M+H]+, 6.0%, corresponding to the molecular weight of the molecular formula C34H34N12O6 of the assigned structure), 691 (708-OH, 12.3%), 674 (708-2OH, 24.7%), 663 (708-OCH2CH3, 7.9%), 635 (708- EtOOC, 23.8%), 590 (708-EtO, COOEt, 7.1%), 562 (708-4COOEt, 42.1%), 519 (708-2(COOEt)2, COCH3, 61.3%), 476 (800-2(COOEt)2, 2COCH3, 100%); Anal. for C34H32N10O8 (708.7) Calcd./Found (%): C (57.62/ 57.70%) H (4.55/4.53%) N (19.76/19.72%).

2.6. Synthesis of 14 and 15

A solution of 4 (1.64 g, 3 mmole) and 2-bromo-1-phenyl-ethanone, 2-bromo-1-p-chlorophenylethanone (0.59 g or 0.69 g, 3 mmole) in dimethyl-formamide (10 ml) containing KOH (0.16 g, 3 mmole) was stirred at room temperature for 2 hours. The formed precipitates were collected by filtration, washed with water and crystallized to give 14 and 15.

2,2’-(4,4’-(1,4-Phenylene)bis(5-acetyl-3-amino-6-methyl-1H-pyrazolo-[3,4-b]pyridine-4,1-diyl))bis(1-phenylethanone) (14): as yellow crystals, yield 44%, mp > 300˚C, IR (ν∙cm−1): 3354, 3262 (NH2), 2873 - 2987 (C-H aliphatic), 3070 (aromatic-CH)), 1705 (CO-benzoyl) and 1668 (CO-Acetyl); 1HNMR (dppm): 1.72 (s, 6H, two CH3 at pyridine), 2.55 (s, 6H, 2CH3CO), 4.01 (s, br., 4H, 2NH2), 5.01 (s, 4H, 2CH2CO), 7.1 - 8.3 (m, 14H, phenylH’s); MS: 691 ([M+H]+, 0.6%, corresponding to the molecular weight of the molecular formula C40H34N8O4 of the assigned structure), 689 (690-H, 10.8%), 688 (690-2H, 12.1%), 663 (708-OCH2CH3, 7.9%), 635 (708- EtOOC, 23.8%), 590 (708-EtO, COOEt, 7.1%), 562 (708-4COOEt, 42.1%), 519 (708-2(COOEt)2, COCH3, 61.3%), 476 (800-2(COOEt)2, 2COCH3, 100%); Anal. for C40H34N8O4 (690.7) Calcd./Found (%): C (69.55/ 69.57%) H (4.96/4.94%) N (16.22/16.24%).

2,2’-(4,4’-(1,4-Phenylene)bis(5-acetyl-3-amino-6-methyl-1H-pyrazolo[3,4-b]pyrid-ine-4,1-diyl))bis(1-(4-chlorophenyl)ethanone) (15): as yellow crystals, yield 44%, mp > 300˚C, IR (ν∙cm−1): 3347, 3253 (NH2), 2878 - 2967 (C-H aliphatic), 3075 (aromatic-CH)), 1700 (CO-benzoyl) and 1672 (CO-Acetyl); 1HNMR (dppm): 1.67 (s, 6H, two CH3 at pyridine), 2.59 (s, 6H, 2CH3CO), 4.05 (s, br., 4H, 2NH2), 5.12 (s, 4H, 2CH2CO), 7.0 - 8.1 (m, 12H, phenyl H’s); MS: 760 ([M+H]+, 2.1%, corresponding to the molecular weight of the molecular formula C40H32Cl2N8O4 of the assigned structure), 761 (M+ +2689 (690-H, 10.8%), 688 (690-2H, 12.1%), 663 (708- OCH2CH3, 7.9%), 635 (708-EtOOC, 23.8%), 590 (708-EtO, COOEt, 7.1%), 562 (708-4COOEt, 42.1%), 519 (708-2(COOEt)2, COCH3, 61.3%), 476 (800-2(COOEt)2, 2COCH3, 100%); Anal. for C40H32Cl2N8O4 (759.6) Calcd./Found (%): C (63.24/63.27%) H (4.25/4.24%) Cl (9.33/9.34) N (14.75/14.74%).

3. Results and Discussion

An unequivocal support for the structure of compound 1 [20] came from the series of reactions concerning the presence and synthetic potentiality of NH, C=S and CN functional groups in 4,4’-(1,4-phenylene)bis(5-acetyl- 6-methyl-2-thioxo-1,2-dihydro-pyridine-3-carbonitrile) 1 and this stimulated our interest to use it as the good starting material for the present study aiming for further chemical transformation required for our medicinal chemistry programs. Thus, it has been found that 1 reacted with methyl iodide in sodium methoxide under stirring at room temperature for 15 minutes to give the corresponding 2-methylthio derivative 2. The IR (cm−1) and 1HNMR (dppm) of 2 showed neither bands nor signals for NH groups and instead that of SCH3 groups were detected. Moreover, its mass spectrum gave the parent peak at m/z = 486, which corresponding to the molecular weight of the molecular formula C26H22N4O2S2 of the assigned structure 2 (cf. Experimental Part). The neighboring and the chemical reactivity of both SCH3 and CN functions in 2 were investigated via its reaction with hydrazine hydrate under reflux till the odor of methyl mercaptan ceased. The reaction seemed to proceeded via the cleavage of SCH3 groups simultaneously with the ring closure of 2-hydrazino derivatives to afford the corresponding 1,1’-(4,4’-(1,4-phenylene)bis(3-amino-6-methyl-1H-pyrazolo[3,4-b]pyridine-5,4-diyl))diethanone 3. Authentically, compound 3 obtained via the reaction of 1 with hydrazine hydrate under reflux through the removing of H2S molecule. The IR (cm−1) of 3 showed no absorption bands of CN function and instead the newly formed NH2 function was detected. The elemental analyses and 1H NMR spectral data used to elucidate the structures of this reaction product (cf. Experimental Part). Moreover, its mass spectrum gave the parent peak (M+) at m/z = 454.19% which corresponding to the molecular weight of the molecular formula C24H22N8O2 of the assigned structure 3. In addition to the parent peaks (M+), other peaks at m/z = (M+-H), (M+-2H) and (M+- NH2) which gave the further confirmation of the 3 structure (cf. Experimental Part and Scheme 1).

The chemical reactivity of NH2 in 3 was investigated via its reaction with nitrous acid under stirring at room temperature to give the corresponding diazonium salt 4 which in turn, used as the good coupling reagents to synthesize several pyridopyrazolo-triazines (cf. Experimental Part). Thus, compound 4 was reacted with malononitrile and 2-cyanoethane-thioamide in ethanol (30 mL) containing sodium acetate (2 g) under stirring at room temperature for 1h to give the corresponding 10,10’-(1,4-phenylene)bis(9-acetyl-4-amino-8-methyl-6,10b-di- hydropyrido[2’,3’:3,4]-pyrazolo[5,1-c][1,2,4]triazine-3-carbonitrile) 5 and 10,10’-(1,4-phenyl-ene)bis(9-acetyl- 4-amino-8-methyl-6,10b-dihydropyrido[2’,3’:3,4]pyrazolo-[5,1-c][1,2,4]triazine-3-carbothioamide) 6 respectively.

Scheme 1. Synthesis of compound 2-4.

The IR (cm−1) spectrum of 5 showed the bands of CN and NH2 functions while that of 6 showed no absorption bands of CN function and instead CSNH2 functions were detected. Also, the 1H NMR (δppm) spectra of each of 5 and 6 revealed the signals of NH2, pyridine and aromatic protons. Compound 6 further confirmed via the presence of S by the element test. In addition to the elemental analyses data, the mass spectra of each of 5 and 6 gave the parent peaks (M+) at m/z = 612.22 and 680.20 which corresponding to the molecular weights of the molecular formulas C30H24N14O2 and C30H28N14O2S2 of the assigned structures 5,6 respectively and such results used as good evidence for further confirmation of their structures (cf. Scheme 2 and Experimental Parts).

In a similar behavior, compound 4 was reacted with ethyl 3-oxobutanoate to give ethyl 10,10’-(1,4-pheny- lene)bis(9-acetyl-4,8-dimethyl-6,10b-di-hydropyrido[2’,3’:3,-4]pyrazolo[5,1-c][1,2,4]triazine-3-carboxylate)7. The mass spectra of this reaction product gave the parent peak (M+) at 704.28% which corresponding to the molecular weight of the molecular formula C36H36N10O6 of the assigned structure 7. Moreover, the 1HNMR (δppm) spectral data of this reaction product revealed the signals of -COOCH2CH3 protons (cf. Scheme 2 and Experimental Part). Similarly, compound 4 was reacted with pentan-2,4-dione in ethanol (30 mL) containing sodium acetate (2 gm) under stirring at room temperature for 1h to give the reaction product 8. The IR (cm−1) of this reaction product showed the absorption band of CH3CO groups. Moreover, its mass spectrum gave the parent peak (M+) at m/z = 644 which corresponding to the molecular weight of the molecular formula C34H32N10O4 for the assigned structure 8. Also, several peaks at m/z = 629 (M+-CH3) and 601 (M+-COCH3). Considering the above mentioned compound 8 formulated as 1,1’-(10,10’-(1,4-phenylene)bis(3,8-dimethyl)-6,10b-dihydropyri- do[2’,3’:3,4]pyrazolo[5,1-c][1,2,-4]triazine-10,9-diyl)-diethanone (cf. Experimental Part Scheme 3). Also, com- pound 4 reacted with ethyl 3-oxo-3-phenylpropanoate to give the reaction product 10,10’-(1,4-phenylene) bis(3-benzoyl-4-hydroxy-8-methyl-6,10b-dihydropyrido[2’,3’:3,4]-pyrazolo[5,1-c][1,2,4]triazine-10,9-diyl))diethanone 9. The1H NMR spectra revealed the signal of OH and phenyl protons. Also, the mass spectra of this

Scheme 2. Synthesis of compound 5-7.

reaction product gave the parent peak at m/z = 772.25 which corresponding to the molecular weight of the molecular formula C42H32N10O6 of the assigned structure 9 (cf. Scheme 3 and Experimental Part).

Moreover, compound 4 reacted with diethylmalonate in ethanol (30 mL) containing sodium acetate (2 g) under stirring at room temperature for 1h to give the corresponding tetraethyl 2,2’-(2,2’-(4,4’-(1,4-pheny- lene)bis(5-acetyl-6-methyl-2,3-dihydro-1H-pyrazolo[3,4-b]pyridine-4,3-diyl))bis-(hydrazin-2-yl-1-ylidene))dimalonate 10. The IR of this reaction product showed the absorption bands of ester CO function and 1HNMR spectra revealed the signals of COOCH2CH3Moreover, the mass spectrum gave the parent peak at m/z = 800.32% which corresponding to the molecular weight of the molecular formula C38H44N10O10 of the assigned structure 10 in addition to several peaks that gave further confirmation of this structure (cf. Scheme 4 and Experimental Part). Thus, it has been found that compound 4 reacted with diethylmalonate in ethanol (30 mL) containing the catalytic amount of piperidine under reflux to give a new triazine ring corresponding to diethyl 10,10’-(1, 4-phenylene)bis(9-acetyl-4-hydroxy-8-methyl-6,10b-dihydropyrido[2’,3’:3,4]pyrazolo[5,1-c][1,2,-4]triazine-3-c

Scheme 3. Synthesis of compound 8 and 9.

Scheme 4. Synthesis of compound 10 and 11.

arboxylate) 11. The1H NMR spectra revealed the new signal of OH in addition to signals of COOCH2CH3 protons. Also, the mass spectrum of this reaction product gave the parent peak at m/z = 708.24% which corresponding to the molecular weight of the molecular formula C34H32N10O8 of the assigned structure 11 (cf. Scheme 4 and Experimental Part). In a similar behavior, ethyl cyanoacetate in ethanol (30mL) containing sodium acetate (2g) under stirring at room temperature for 1 h to afford the corresponding reaction product diethyl 2,2’- (2,2’-(4,4’-(1,4-phenyl-ene)bis(5-acetyl-6-methyl-2,3-dihydro-1H-pyrazolo[3,4-b]pyrid-ine-4,3-diyl))bis-(hydrazin-2-yl-1-ylidene))bis(3-cyanopropanoate) 12. The IR (cm−1) of this reaction product showed the absorption band of each of ester carbonyl and CN and on the other hand, its 1HNMR (δppm) spectrum revealed the signals of -COOCH2CH3 protons. Moreover, the mass spectrum gave the parent peak at m/z = 734.30 which corresponding to the molecular weight of the molecular formula C36H38N12O6 of the assigned structure 12. (cf. Scheme 5 and Experimental Part). Thus, it has been found that compound 4 reacted with ethyl cyanoacetate in ethanol (30 mL) containing the catalytic amount of piperidine under reflux to give the corresponding reaction product diethyl 10,10’-(1,4-phenylene)bis(9-acetyl-4-amino-8-methyl-6,10b-dihydropyrido[2’,3’-:3,4]pyrazolo [5,1-c][1,2, 4]triazine-3-carboxylate) 13.

Scheme 5. Synthesis of compound 12 and 13.

Scheme 6. Synthesis of compound 14 and 15.

The IR (cm−1) of this reaction product showed the absorption band of each of ester carbonyl and NH2 and on the other hand, its 1HNMR (δppm) spectrum revealed the signals of -COOCH2CH3 and NH2 protons and this confirmed that the cyclization proceeded via addition on the CN function. Also, the mass spectrum of this reaction product gave the parent peak at m/z = 706.27% which corresponding to the molecular weight of the molecular formula C34H34N12O6 of the assigned structure 13 (cf. Scheme 5 and Experimental Part).

The chemical reactivity and synthetic potential of 4 were investigated via its chemical reaction with 2-bro- mo-1-phenylethanone and 2-bromo-1-(4-chlorophenyl)ethanone in dimethylformamide (10 mL) containing potassium hydroxide (1mmole) under stirring at room temperature for 1 h to afford the corresponding reaction product 2,2’-(4,4’-(1,4-phenylene)-bis(5-acetyl-3-amino-6-methyl-1H-pyrazolo[3,4-b]pyridine-4,1-diyl))bis-(1- phenylethanone) and 2,2’-(4,4’-(1,4-phenylene)bis(5-acetyl-3-amino-6-methyl-1H-pyrazolo[3,4-b]pyridine-4,1- diyl))bis(1-(4-chlorophenyl)ethanone) 14 and 15 respect-ively. The IR (cm−1) of this reaction product showed the absorption band of NH2 function as well as its 1HNMR (δppm) spectrum revealed the signals of -NH2 protons. Moreover, their mass spectra gave the parent peaks at m/z = 690.27% and 758.19% in respective manner which corresponding to their molecular weights of the molecular formulas C40H34N8O4 and C40H32Cl2N8O4 of the assigned structures 14 and 15 (cf. Scheme 6 and Experimental Part).

Cite this paper

Ahmed A. M. Elreedy,Hameed M. Alkubaisi,Fawzy A. Attaby, (2016) Synthesis, Reactions and Characterization of 1,1’-(1,4-Phenylenebis(3-amino-6-methyl-1H-pyrazolo[3,4-b]pyridine-4,5-diyl))bis(ethan-1-one). International Journal of Organic Chemistry,06,65-76. doi: 10.4236/ijoc.2016.61007

NOTESReferencesSingh, H., Yadav, L.D.S. and Bhattacharya, B.K. (1979) Synthesis of Some New Bis(1,2,4-triazol-3-yl)disulfides, Sulfides and Sulfones as Potential Pesticides. J. Indian. Chem. Soc., 56, 1013.Desai, N.C. (1993) Synthesis and Antimicrobial Activity of Some Dithiocarbamates, 2-Arylamino-4-oxothiazoles and Their 5-Arylidine Derivatives. Indian Journal of Chemistry—Section B, 32, 343.Yahia, N.M., Assem, B., Abdullah, M., Saeed, A., Sammer, Y. and Choudhary, M. (2013) Synthesis, Reactions and Biological Activity of Some New Bis-Heterocyclic Ring Compounds Containing Sulphur Atom. Chemistry Central Journal, 7, 112.Upadhyay, P.S., Vansdadia, R.N. and Baxi, A.J. (1990) Studies on Sulphone Derivatives: Preparation and Antimicrobial Activity of Thiosemicarbazides, Thiazolidones, Triazoles, Oxadiazoles and Thiadiazoles. Indian Journal of Chemistry—Section B, 29, 793.Zhang, Z.Y., Chen, X., Wei, L.L. and Ma, Z.L. (1991) Studies on Condensed Heterocyclic Compounds (Ⅲ) Synthesis and Antibacterial Activity of 3-(4-Pyridyl)-6-Alkyl/Aromatic Heterocyclyl-s-Tri-azolo[3,4-b]-1,3,4-Thiadiazoles and 1,4-Bis [6-(4-Pyridyl)-s-Triazolo[3,4-b]-1, 3,4-Thiadiazol-3-yl]-n-Bu. Chem. Res. Chin. Univ., 7, 129.Bhat, A.R., Athar, F. and Azam, A. (2009) Bis-Pyrazolines: Synthesis, Characterization and Antiamoebic Activity as Inhibitors of Growth of Entamoeba histolytica. European Journal of Medicinal Chemistry, 44, 426-431. http://dx.doi.org/10.1016/j.ejmech.2007.11.005Kerru, N., Nallapaneni, H., Kasturi, V. and Rao, C.V. (2015) Synthesis and Biological Activity of Novel Bis and Mono Heterocycles of Thienopyrimidine Derivatives. Indo American Journal of Pharmaceutical Research, 5, 1604.Iqbal, P.F., Parveen, H., Bhat, A.R., Hayat, F. and Azam, A. (2009) Synthesis, Characterization, Antiamoebic Activity and Toxicity of Novel Bisdioxazole Derivatives. European Journal of Medicinal Chemistry, 44, 4747-4751. http://dx.doi.org/10.1016/j.ejmech.2009.06.016Dawoud, N.T.A. (2011) Synthesis, Reactions and Antimicrobial Activity of Some Substituted 4,6-Diphenyl Pyridine 2-Thione Derivatives. Nature and Science, 9, 202.Madkour, H.M.F., Salem, M.A.L., Marzouk, M.L., Azab, M.E. and Mahmoud, N.F.H. (2007) Utility of Cyanothioacetamide in the Synthesis of Pyrazolo[4,3-c], Isoxazolo[4,5-c], Thieno[2,3-b] and Furo[2,3-c] of Thioxopyridine Derivatives and their Antibacterial Activities. American-Eurasian Journal of Scientific Research, 2, 161-169.Paronikyan, E.G., Akopyan, Sh.F., Noravyan, A.S., Dzhagatspanyan, L.A., Nazaryan, L.M. and Akopyan, A.G. (2010) Synthesis and Neurotropic Activity of 6-Thio-Substituted Pyrano[3,4-c]pyridine and 1-Aminopyrano[4,3-d]-thieno [2,3-b]pyridine Derivatives and 9-Substituted Pyrido[2,3-b]thieno[3,2-d]pyrimidines. Pharmaceutical Chemistry Journal, 44, 183-185. http://dx.doi.org/10.1007/s11094-010-0426-5Abbas, A.A., Elneairy, M.A.A. and Mabkhot, Y.N. (2001) Versatile Starting Materials for Novel 1,ω-Bis(pyridin-4-ylphenoxy)alkanes, and Their Corresponding Bis-(thieno[2,3-b]pyridin-4-ylphenoxy) Derivatives. Journal of Chemical Research, 4, 124-126. http://dx.doi.org/10.3184/030823401103169388Bhat, A.R., Ather, F. and Azam, A. (2009) Bis-Pyrazolines: Synthesis, Characterization and Antiamoebic Activity as Inhibitors of Growth of Entamoeba histolytica. European Journal of Medicinal Chemistry, 44, 426-431. http://dx.doi.org/10.1016/j.ejmech.2007.11.005Griffin, A.C. and Britt, T.R. (1981) Effect of Molecular Structure on Mesomorphism. 12. Flexible-Center Siamese- Twin Liqiud Crystalline Diesters-a “Prepolymer” Model. Journal of the American Chemical Society, 103, 4957-4959. http://dx.doi.org/10.1021/ja00406a056Galli, G., Laus, M. and Angeloni, A.S. (1986) Synthesis and Thermotropic Properties of New Mesogenic Diacrylate Monomers. Die Makromolekulare Chemie, 187, 289-296. http://dx.doi.org/10.1002/macp.1986.021870206Ringsdorf, H., Schlarb, B. and Venzmer, J. (1988) Molecular Architecture and Function of Polymeric Oriented Systems: Models for the Study of Organization, Surface Recognition, and Dynamics of Biomembranes. Angewandte Chemie International Edition in English, 27, 113-158. http://dx.doi.org/10.1002/anie.198801131Finkelman, H. (1987) Liquid Crystalline Polymers. Angewandte Chemie International Edition in English, 26, 816-824. http://dx.doi.org/10.1002/anie.198708161Aguilera, C., Parra, M. and Fuentes, G.Z. (1998) Synthesis and Mesomorphic Properties of Polymethylene-bis[2-thio- 5-(4’,4’’n-alcoxybenzoyloxy)phenyl]-1,3,4-oxadiazole. Zeitschrift für Naturforschung, 53b, 367-370.Braun, D. and Langendorf, R. (1999) Vitrigens. I. Synthesis and Characterization of Low Molecular Weight Organic Glasses. Journal für Praktische Chemie, 341, 128-137. http://dx.doi.org/10.1002/(SICI)1521-3897(199902)341:2<128::AID-PRAC128>3.0.CO;2-MAbdel-Fattah, A.M. and Elsayed, M.M. (2009) Novel Synthesis, Reactions and Anti-Cancer Activities of 4,4’-Ben- zene-1,4-diylbis(6-phenyl-2-thioxo-1,2-dihydro-pyridine-3-carbonitrile). Current Organic Chemistry, 13, 1751-1757. http://dx.doi.org/10.2174/138527209789578036Shestopalov, A.M., Nikishin, K.G., Gromova, A.V. and Rodinovskaya, L.A. (2003) One-Pot Synthesis of 4,6-Diaryl-3-cyanopyridine-2(1H)-thiones and Their Transform-Ation to Substituted Thieno[2,3-b;4,5-b]dipyridines and Pyrido[3’’,2’’:4,5]thieno-[3,2-d]pyrimidines. Russian Chemical Bulletin, 52, 2203-2206.Prachayasittikul, S., Treeratanapiboon, L., Ruchirawat, S. and Prachayasittikul, V. (2009) Novel Activities of 1-Ada-mantylthiopyridines as Antibacterials, Antimalarials and Anticancers. EXCLI Journal, 8, 121-129.Al-Abdullah, E.S. (2011) Synthesis and Anticancer Activity of Some Novel Tetralin-6-yl-pyrazoline, 2-Thioxopyrimi- dine, 2-Oxopyridine, 2-Thioxopyridine and 2-Iminopyridine Derivatives. Molecules, 16, 3410.Attaby, F.A. and Alkubaisi, H.M. (2015) Synthesis, Reactions and Characterization of 6-Thioxo-1,6-dihydro-2,3’-bi- pyridine-5-carbonitrile. Journal of Purity, Utility Reaction and Environment, 4, 1-15.Attaby, F.A., Abdel-Fattah, A.M., Shaif, L.M. and Elsayed, M.M. (2010) Anti-Alzheimer and Anti-COX-2 Activities of the Newly Synthesized 2,3’-Bipyridine Derivatives (II). Phosphorus, Sulfur and Silicon and the Related Elements, 185, 668-679. http://dx.doi.org/10.1080/10426500902917644Attaby, F.A., Abdel-Fattah, A.M., Shaif, L.M. and Elsayed, M.M. (2010) Anti-Alzheimer and Anti-COX-2 Activities of the Newly Synthesized 2,3’-Bipyridine Derivatives (I). Phosphorus, Sulfur and Silicon and the Related Elements, 185, 129-139. http://dx.doi.org/10.1080/10426500902717333Attaby, F.A., Elghandour, A.H., Ali, M.A. and Ibrahem, Y.M. (2007) Synthesis, Characterization and Antiviral Activities of Pyridopyrazolotriazines. Phosphorus, Sulfur and Silicon and the Related Elements, 182, 133-149. http://dx.doi.org/10.1080/10426500600887313Attaby, F.A., Elghandour, A.H.H., Ali, M.A. and Ibrahem, Y.M. (2007) Synthesis, Reactions and Antiviral Activity of 6’-Amino-2’-thioxo-1’,2’-dihydro-3,4’-bipyridine-3’,5’-dicarbonitrile. Phosphorus, Sulfur and Silicon and the Related Elements, 182, 695-709. http://dx.doi.org/10.1080/10426500601087277Attaby, F.A., Ramla, M.M. and Gouda, E.M. (2007) Synthesis, Reactions, and Biological Activity of 4(1H-Indol-3-yl)-2-Thioxopyridine Derivatives. Phosphorus, Sulfur and Silicon and the Related Elements, 182, 517-528. http://dx.doi.org/10.1080/10426500601013216Attaby, F.A., Abdel-Fattah, A.M., Shaif, L.M. and Elsayed, M.M. (2009) Anti-Alzheimer and Anti-COX-2 Activities of the Newly 2-Substituted thienopyridines. Current Organic Chemistry, 14, 1654-1663. http://dx.doi.org/10.2174/138527209789578135Attaby, F.A., Abdel-Fattah, A.M., Shaif, L.M. and Elsayed, M.M. (2010) Reactions, Anti-Alzheimer and Anti COX-2 Activities of 6-Pyridin-3-yl-1H-pyrazolo[3,4-b]pyridin-3-amines. Current Organic Chemistry, 14, 2522-2530. http://dx.doi.org/10.2174/138527210793358303Attaby, F.A., Said, M.M. and Bakr, S.A.M. (2013) Synthesis, Reactions and Characterization of 4,4’-Benzene-1,4-diylbis(5-Acetyl-6-methyl-2-thioxo-1,2-dihydropyridine-3-carbonitrile). Egyptian Journal of Chemistry, 56, 127-140.Attaby, F.A. and Elreedy, A.A.M. (2014) Synthesis, Reactions and Characterization of Bisthieno[2,3-b]pyridine-2- carbohydrazide Derivative. International Journal of Scientific & Engineering Research, 5, 662-671.