<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">FNS</journal-id><journal-title-group><journal-title>Food and Nutrition Sciences</journal-title></journal-title-group><issn pub-type="epub">2157-944X</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/fns.2016.72014</article-id><article-id pub-id-type="publisher-id">FNS-63944</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biomedical&amp;Life Sciences</subject></subj-group></article-categories><title-group><article-title>
 
 
  The Phenolic Potential of Wines from French Grape Varieties Cabernet Sauvignon, Merlot and Syrah Cultivated in the Region of Thessaloniki (Northern Greece) and Its Evolution during Aging
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>onstantina</surname><given-names>Stavridou</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Evangelos</surname><given-names>H. Soufleros</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Elisavet</surname><given-names>Bouloumpasi</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Vagia</surname><given-names>Dagkli</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib></contrib-group><aff id="aff2"><addr-line>Department of Oenology and Beverage Technology, Eastern Macedonia and Thrace Institute of Technology,
Drama, Greece</addr-line></aff><aff id="aff1"><addr-line>Department of Food Science &amp;amp; Technology, Faculty of Agronomy, Aristotle University of Drama,
Thessaloniki, Greece</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>esoufler@agro.auth.gr(EHS)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>16</day><month>02</month><year>2016</year></pub-date><volume>07</volume><issue>02</issue><fpage>122</fpage><lpage>137</lpage><history><date date-type="received"><day>12</day>	<month>December</month>	<year>2015</year></date><date date-type="rev-recd"><day>accepted</day>	<month>26</month>	<year>February</year>	</date><date date-type="accepted"><day>29</day>	<month>February</month>	<year>2016</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
   The aim of this work is mainly to determine the effect of the pedoclimatic conditions (“terroir”) on the phenolic composition of wine originated from three French red grape varieties Cabernet Sauvignon, Merlot and Syrah, cultivated in the region of Thessaloniki (Northern Greece); simultaneously a study of its evolution during aging in a stainless steel tank, in oak barrels of different origin and in bottles is conducted. Additionally, in this work, we try to compare the evolution of phenolic composition of wines during aging in French medium toast oak barrel and in American heavy toast oak barrel. For this reason, 20 red wine samples originated from this area were analyzed. The color markers were measured by UV-spectrometry, while the phenolic acids and catechin content of the wine samples and their evolution during aging were determine by UVis-High performance liquid chromatography. Significant differences detected among the three grape varieties regarding color parameters, total anthocyanins and some phenolic acids. The increase of the extraction time during vinification affected the amounts of phenolic acids, catechin, total phenolic index (TPI) and tannin content. Gallic acid and catechin were the most abundant phenolic compounds and their amounts, as well as the concentrations of several phenolic acids were significantly affected during the storage period. Finally, the phenolic composition and the amounts of phenolic acids did not appear to be affected by the origin of oak barrels. 
 
</p></abstract><kwd-group><kwd>Phenolics</kwd><kwd> Wine</kwd><kwd> Aging</kwd><kwd> French</kwd><kwd> Varieties</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>Studies have shown that the wine phenolic compounds originate mainly from grape extraction during vinification [<xref ref-type="bibr" rid="scirp.63944-ref1">1</xref>] and to a less extent from the wood barrel during maturation or even from yeast metabolism [<xref ref-type="bibr" rid="scirp.63944-ref2">2</xref>] [<xref ref-type="bibr" rid="scirp.63944-ref3">3</xref>] or other microbial sources [<xref ref-type="bibr" rid="scirp.63944-ref4">4</xref>] [<xref ref-type="bibr" rid="scirp.63944-ref5">5</xref>] .</p><p>It is well known that phenolic compounds are important ingredients of wine and affect several sensory attributes, such as color and astringency [<xref ref-type="bibr" rid="scirp.63944-ref6">6</xref>] [<xref ref-type="bibr" rid="scirp.63944-ref7">7</xref>] . With regard to the influence of phenolic compounds upon wine color, it has been shown that anthocyanins have the stronger effect on the color of young red wines. The concentration of anthocyanins in young red wines ranges between 200 and 500 mg/L, while decreases to 10 to 20 mg/L during aging [<xref ref-type="bibr" rid="scirp.63944-ref7">7</xref>] . Red wines contain 1500 to 2500 mg/L of total phenolic compounds [<xref ref-type="bibr" rid="scirp.63944-ref8">8</xref>] . In general, the color of aged red wines, as well as the chemical and sensory profile results from different combinations of the phenolic compounds [<xref ref-type="bibr" rid="scirp.63944-ref9">9</xref>] - [<xref ref-type="bibr" rid="scirp.63944-ref12">12</xref>] .</p><p>On the other hand, the development of the astringent taste of red wines is due to the concentrated tannins (catechins), which are very important for the long term stabilization of color [<xref ref-type="bibr" rid="scirp.63944-ref13">13</xref>] - [<xref ref-type="bibr" rid="scirp.63944-ref15">15</xref>] . The astringent taste of red wines results from the reaction of saliva proteins (proline rich) with the wine tannins [<xref ref-type="bibr" rid="scirp.63944-ref16">16</xref>] .</p><p>Antioxidant and antibacterial activity of phenolics has been well established [<xref ref-type="bibr" rid="scirp.63944-ref7">7</xref>] . Flavonoids act as antioxidants and protect plant DNA damage from ultraviolet irradiation. Regarding human health, it is shown that polyphenols protect from cardiovascular diseases. Polyphenols of wines are more biologically available because they exist in a diluted form whereas, in plants, they are either polymerized or insoluble or even strongly bound to other matrices. Moderate wine consumption is associated with decreased cardiovascular diseases even in nations that are well known to follow a high fat consumption diet (French paradox) [<xref ref-type="bibr" rid="scirp.63944-ref17">17</xref>] - [<xref ref-type="bibr" rid="scirp.63944-ref19">19</xref>] . On the other hand, moderate wine consumption is shown to reduce the incidents of senile dementia and Alzheimer disease [<xref ref-type="bibr" rid="scirp.63944-ref20">20</xref>] .</p><p>Phenolic acids present antibiotic and antiseptic attributes thus are used for food preservation. It is likely that these acids play some role in the microbiological stability of wine, mainly towards bacteria. Those phenolic acids that posses two phenolic hydroxyls (-OH) in ortho-position, such as gallic acid, have the attribute to easily oxidise in quinones, affecting the color of white wines [<xref ref-type="bibr" rid="scirp.63944-ref7">7</xref>] . One of the most important phenolic acids is p-coum- aric acid, provided that it is an important metabolite in the formation of phenylalanine lyase, an enzyme that is involved in the formation of flavonoids and stilbenes. It has been found that coumaric acid concentrations increase during stress periods of vine [<xref ref-type="bibr" rid="scirp.63944-ref21">21</xref>] . Furthermore, hydroxycinnamic acids, where p-coumaric acid belongs, form complexes with anthocyanins (pyranoanthocyanins), which contribute to the color stabilization of wine during aging [<xref ref-type="bibr" rid="scirp.63944-ref9">9</xref>] [<xref ref-type="bibr" rid="scirp.63944-ref22">22</xref>] .</p><p>The purpose of this study is to assess and compare several color markers and phenolic acid concentrations of wine made from different grape varieties, as well as wines vinified with different techniques or aged under diverse conditions.</p></sec><sec id="s2"><title>2. Materials and Methods</title>
<sec id="s2_1"><title>2.1. Wine Samples and Terroir</title><p>In the present work 20 wine samples were studied (<xref ref-type="table" rid="table1">Table 1</xref>). The wines were produced from the French grape varieties Cabernet Sauvignon, Merlot and Syrah, cultivated in the region of Thessaloniki (Northern Greece) and more precisely on the slopes of village Trilofos (vinification at 2004). The soil of this region is classified as CL to a depth of 30 cm, from 30 - 60 cm as SCL and from 60 - 90 cm as SL. The top soil (0 - 30 cm) is characterized as moderate alkaline, moderate in CaCO<sub>3</sub> content (9.2%) and low in organic matter (0.41%) content. The concentrations of P, Ca and Mg are elevated, while the concentrations of K, Mn and Zn very low (<xref ref-type="table" rid="table2">Table 2</xref>).</p><p>All French grape varieties were classically vinified after a six-day extraction period except of the Merlot-c wine, which was extracted for three weeks. All wines were stored initially in a stainless steel tank for 4 months, transferred in barrels for 2 months and finally were bottled in order to study the evolution of color markers during aging. The Merlot-a<sub> </sub>and Syrah-d<sub> </sub>wines (<xref ref-type="table" rid="table1">Table 1</xref>) were stored for 2 months in heavy toast American oak</p></sec></sec></body>
<back><ref-list><title>References</title><ref id="scirp.63944-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">Borbalan, A., Angeles, M., Zoro, L., Guillen, D.A. and Barroso, C.G. (2003) Study of the Polyphenol Content of Red and White Grape Varieties by Liquid Chromatography-Mass Spectometry and Its Relationship to Antioxidant Power. Science Direct—Journal of Chromatography A, 1012, 31-38. &lt;/br&gt;http://dx.doi.org/10.1016/S0021-9673(03)01187-7</mixed-citation></ref><ref id="scirp.63944-ref2"><label>2</label><mixed-citation publication-type="other" xlink:type="simple">Burns, J., Gardner, P.T., O’Neil, J., Crawford, S., Morecroft, I., McPhall Donald, B., Lister, C., Matthews, D., MacLean, M.R., Lean Michael, E.J., Duthie Garry, G. and Crozier, A. (2000) Relationship among Antioxidant Activity, Vasolidation Capacity and Phenolic Content of Red Wines. American Chemical Society. Journal of Agricultural and Food Chemistry, 48, 220-230. &lt;/br&gt;http://dx.doi.org/10.1021/jf9909757</mixed-citation></ref><ref id="scirp.63944-ref3"><label>3</label><mixed-citation publication-type="other" xlink:type="simple">Burns, J., Gardner, P.T., Matthews, D., Duthie Garry, G., Lean Michael, E.J. and Crozier, A. (2001) Extraction of Phenolics and Changes in Antioxidant Activity of Red Wines during Vinification. Journal of Agricultural and Food Chemistry, 49, 5797-5808. &lt;/br&gt;http://dx.doi.org/10.1021/jf010682p</mixed-citation></ref><ref id="scirp.63944-ref4"><label>4</label><mixed-citation publication-type="other" xlink:type="simple">Kennedy, J.A. (2008) Grape and Wine Phenolics: Observations and Recent Findings. Ciencia e Investigación Agrarian, 35, 107-120. &lt;/br&gt;http://dx.doi.org/10.4067/s0718-16202008000200001</mixed-citation></ref><ref id="scirp.63944-ref5"><label>5</label><mixed-citation publication-type="other" xlink:type="simple">Silva, L.R., Andrade, P.B., Valent&amp;atildeo, P., Seabra, R.M., Trujillo, M.E. and Velázquez, E. (2005) Analysis of Non-Coloured Phenolics in Red Wine: Effect of Dekkera Bruxellensis Yeast. Food Chemistry, 89, 185-189. &lt;/br&gt;http://dx.doi.org/10.1016/j.foodchem.2004.02.019</mixed-citation></ref><ref id="scirp.63944-ref6"><label>6</label><mixed-citation publication-type="other" xlink:type="simple">Oberholster, A. (2003) Chemical and Sensory Properties of Grape and Wine Phenolics. Part I. Wynboer—Technical Articles, 1-13.</mixed-citation></ref><ref id="scirp.63944-ref7"><label>7</label><mixed-citation publication-type="other" xlink:type="simple">Soufleros, E. (2015) Enology—Science &amp; Know-How. Thessaloniki. (In Greek)</mixed-citation></ref><ref id="scirp.63944-ref8"><label>8</label><mixed-citation publication-type="other" xlink:type="simple">Frankel, E.N., Kanner, J., German, J.B., Parks, E. and Kinsella, J.E. (1993) Inhibition of Oxidation of Human Low-Density Lipoprotein by Phenolic Substances in Red Wine. The Lancet, 341, 454-457. &lt;/br&gt;http://dx.doi.org/10.1016/0140-6736(93)90206-V</mixed-citation></ref><ref id="scirp.63944-ref9"><label>9</label><mixed-citation publication-type="other" xlink:type="simple">Schwarz, M., Hofmann, G. and Winterhalter, P. (2004) Investigations on Anthocyanins in Wines from Vitis vinifera cv. Pinotage: Factors Influencing the Formation of Pinotin A and Its Correlation with Wine Age. Journal of Agricultural and Food Chemistry, 52, 498-504. &lt;/br&gt;http://dx.doi.org/10.1021/jf035034f</mixed-citation></ref><ref id="scirp.63944-ref10"><label>10</label><mixed-citation publication-type="other" xlink:type="simple">Waterhouse, A.L. and Laurie, V.F. (2006) Oxidation of Wine Phenolics: A Critical Evaluation and Hypotheses. American Journal of Enology and Viticulture, 57, 306-313.</mixed-citation></ref><ref id="scirp.63944-ref11"><label>11</label><mixed-citation publication-type="other" xlink:type="simple">Danilewicz, J.C. and Wallbridge, P.J. (2010) Further Studies on the Mechanism of Interaction of Polyphenols, Oxygen, and Sulfite in Wine. American Journal of Enology and Viticulture, 61, 166-175.</mixed-citation></ref><ref id="scirp.63944-ref12"><label>12</label><mixed-citation publication-type="other" xlink:type="simple">Danilewicz, J.C. (2011) Mechanism of Autoxidation of Polyphenols and Participation of Sulfite in Wine: Key Role of Iron. American Journal of Enology and Viticulture, 62, 319-328. &lt;/br&gt;http://dx.doi.org/10.5344/ajev.2011.10105</mixed-citation></ref><ref id="scirp.63944-ref13"><label>13</label><mixed-citation publication-type="other" xlink:type="simple">Kennedy, J.A., Hayasaka, Y., Vidal, S., Waters, E.J. and Jones, G.P. (2001) Composition of Grape Skin Proanthocyanidins at Different Stages of Berry Development. Journal of Agricultural and Food Chemistry, 49, 5348-5355. &lt;/br&gt;http://dx.doi.org/10.1021/jf010758h</mixed-citation></ref><ref id="scirp.63944-ref14"><label>14</label><mixed-citation publication-type="other" xlink:type="simple">Souquet, J.-M., Cheynier, V., Brossaud, F. and Moutounet, M. (1996) Polymeric Proanthocyanidins from Grape Skins. Phytochemistry, 43, 509-512. &lt;/br&gt;http://dx.doi.org/10.1016/0031-9422(96)00301-9</mixed-citation></ref><ref id="scirp.63944-ref15"><label>15</label><mixed-citation publication-type="other" xlink:type="simple">Vrhovsek, U., Mattivi, F. and Waterhouse, A.L. (2001) Analysis of Red Wine Phenolics: Comparison of HPLC and Spectrophotometric Methods. Vitis, 40, 87-91.</mixed-citation></ref><ref id="scirp.63944-ref16"><label>16</label><mixed-citation publication-type="other" xlink:type="simple">Edelmann, A. and Lendl, B. (2002) Toward the Optical Tongue: Flow-Through Sensing of Tannin-Protein Interactions Based on FTIR Spectroscopy. Journal of the American Chemical Society, 124, 14741-14747. &lt;/br&gt;http://dx.doi.org/10.1021/ja026309v</mixed-citation></ref><ref id="scirp.63944-ref17"><label>17</label><mixed-citation publication-type="other" xlink:type="simple">Amico, V., Napoli, E.M., Renda, A., Ruberto, G., Spatafora, C. and Tringali, C. (2004) Constituents of Grape Pomace from Sicilian Cultivar “Nerello Mascalese”. Food Chemistry, 88, 1-13. &lt;/br&gt;http://dx.doi.org/10.1016/j.foodchem.2004.02.022</mixed-citation></ref><ref id="scirp.63944-ref18"><label>18</label><mixed-citation publication-type="other" xlink:type="simple">Gambelli, L. and Santaroni, G.P. (2004) Polyphenols Content in Some Italian Red Wines of Different Geographical Origins. Journal of Food Composition and Analysis, 17, 613-618. &lt;/br&gt;http://dx.doi.org/10.1016/j.jfca.2003.09.010</mixed-citation></ref><ref id="scirp.63944-ref19"><label>19</label><mixed-citation publication-type="other" xlink:type="simple">Sakkiadi, A.V., Stavrakakis, M.N. and Haroutoumian, S.A. (2001) Direct HPLC Assay of Five Biologically Interesting Phenolic Antioxidants in Varietal Greek Red Wines. Lebensmittel-Wissenschaft &amp; Technologie, 34, 410-413. &lt;/br&gt;http://dx.doi.org/10.1006/fstl.2001.0792</mixed-citation></ref><ref id="scirp.63944-ref20"><label>20</label><mixed-citation publication-type="other" xlink:type="simple">Dartigues, J.F., Berr, C., Helmer, C. and Letenneur, L. (2002) Epidemiologie de la maladie d’Alzheimer. Medicine/ Sciences, 18, 737-743. &lt;/br&gt;http://dx.doi.org/10.1051/medsci/20021867737</mixed-citation></ref><ref id="scirp.63944-ref21"><label>21</label><mixed-citation publication-type="other" xlink:type="simple">Kilinc, E. and Kalkan, H. (2003) High-Performance Liquid Chromatographic Determination of Some Phenolic Acids of Turkish Commercial Wines: An Electrochemical Approach. Journal of Wine Research, 14, 17-23. &lt;/br&gt;http://dx.doi.org/10.1080/0957126032000114982</mixed-citation></ref><ref id="scirp.63944-ref22"><label>22</label><mixed-citation publication-type="other" xlink:type="simple">Remy, S., Fulcrand, H., Labarbe, B., Cheynier, V. and Moutounet, M. (2000) First Confirmation in Red Wine of Products Resulting from Direct Anthocyanin-Tannin Reactions. Journal of the Science of Food and Agriculture, 80, 745-751. &lt;/br&gt;http://dx.doi.org/10.1002/(SICI)1097-0010(20000501)80:6&lt;745::AID-JSFA611&gt;3.0.CO;2-4</mixed-citation></ref><ref id="scirp.63944-ref23"><label>23</label><mixed-citation publication-type="other" xlink:type="simple">Ribéreau-Gayon, P., Glories, Y., Maujean, A. and Dubourdieu, D. (1998) Traité d’oenologie—Tome 2: Chimie du vin. Stabilisation et traitements, Dunod, Editions La Vigne.</mixed-citation></ref><ref id="scirp.63944-ref24"><label>24</label><mixed-citation publication-type="other" xlink:type="simple">Guendez, R., Kallithraka, S., Makris, D. and Kefalas, P. (2005) Determination of Low Molecular Weight Polyphenolic Constituents in Grape (Vitis vinifera sp.) Seed Extracts: Correlation with Antiradical Activity. Food Chemistry, 89, 1-9. &lt;/br&gt;http://dx.doi.org/10.1016/j.foodchem.2004.02.010</mixed-citation></ref><ref id="scirp.63944-ref25"><label>25</label><mixed-citation publication-type="other" xlink:type="simple">Soufleros, E., Stavridou, K. and Dagkli, V. (2011) The Effect of Cluster Thinning on Phenolic Maturity of Vitis vinifera cv. Xinomavro Grapes. Journal International des Sciences de la Vigne et du Vin, 45, 1-9.</mixed-citation></ref><ref id="scirp.63944-ref26"><label>26</label><mixed-citation publication-type="other" xlink:type="simple">Canas, S.M., Conceicao, L., Spranger, M.I. and Belchior, A.P. (2000) Influence of Botanical Species and Geographical Origin on the Content of Low Molecular Weight Phenolic Compounds of Woods Used in Portuguese Cooperage. Holzforschunge, 54, 255-261. &lt;/br&gt;http://dx.doi.org/10.1515/hf.2000.043</mixed-citation></ref><ref id="scirp.63944-ref27"><label>27</label><mixed-citation publication-type="other" xlink:type="simple">Matricardi, L. and Waterhouse, A.L. (1999) Influence of Toasting Technique on Color and Ellagitannins of Oak Wood in Barrels Making. American Journal of Enology and Viticulture, 50, 519-525.</mixed-citation></ref><ref id="scirp.63944-ref28"><label>28</label><mixed-citation publication-type="other" xlink:type="simple">Kourakou-Dragonas, S. (1998) Themata Oinologias. Trochalia, Athens.</mixed-citation></ref><ref id="scirp.63944-ref29"><label>29</label><mixed-citation publication-type="other" xlink:type="simple">Castellari, M., Sartini, E., Fabiani, A., Arfelli, G. and Amati, A. (2002) Analysis of Wine Phenolics by High-Performance Liquid Chromatography Using a Monolithic Type Column. Journal of Chromatography A, 973, 221-227.</mixed-citation></ref><ref id="scirp.63944-ref30"><label>30</label><mixed-citation publication-type="other" xlink:type="simple">Perez-Prieto, L.J., De La Hera-Orts, M.L., Lopez-Roca, J.M., Frnandez-Fernandez, J.I. and Gomez-Plaza, E. (2003) Oak-Matured Wines: Influence of the Characteristics of the Barrel on Wine Colour and Sensory Characteristics. Journal of the Science of Food and Agriculture, 83, 1445-1450. &lt;/br&gt;http://dx.doi.org/10.1002/jsfa.1551</mixed-citation></ref><ref id="scirp.63944-ref31"><label>31</label><mixed-citation publication-type="other" xlink:type="simple">Gómez-Cordovés, C. and Gonzalez-SanJosé, M.L. (1995) Interpretation of Color Variables during the Aging of Red Wines: Relationship with Families of Phenolic Compounds. Journal of Agricultural and Food Chemistry, 43, 557-563. &lt;/br&gt;http://dx.doi.org/10.1021/jf00051a001</mixed-citation></ref><ref id="scirp.63944-ref32"><label>32</label><mixed-citation publication-type="other" xlink:type="simple">Jindra, J.A. and Gallender, J.F. (1987) Effect of American and French Oak Barrels on the Phenolic Composition and Sensory Quality of Sevyal Blanc Wine. American Journal of Enology and Viticulture, 38, 133-138.</mixed-citation></ref><ref id="scirp.63944-ref33"><label>33</label><mixed-citation publication-type="other" xlink:type="simple">du Toit, W. (2004) Wood Maturation of Wine (Part II): The Effect of Various Wood Components on the Colour, Flavour and Taste of Wine. Wynboer Technical Articles, WineLand Publications.</mixed-citation></ref><ref id="scirp.63944-ref34"><label>34</label><mixed-citation publication-type="other" xlink:type="simple">Perez-Prieto, L.J., Lopez-Roca, J.M. and Gomez-Plaza, E. (2003) Multivariate Statistical Analysis for the Classification of Oak-Aged Wines Based on Their Chromatic Characteristics. European Food Research and Technology, 217, 512-516. &lt;/br&gt;http://dx.doi.org/10.1007/s00217-003-0780-6</mixed-citation></ref><ref id="scirp.63944-ref35"><label>35</label><mixed-citation publication-type="other" xlink:type="simple">del Alamo, M., Bernal, J.L. and Gomez-Cordoves, C. (2000) Behavior of Monosaccharides, Phenolic Compounds, and Color of Red Wines Aged in Used Oak Barrels and in the Bottle. Journal of Agricultural and Food Chemistry, 48, 4613-4618. &lt;/br&gt;http://dx.doi.org/10.1021/jf9909050</mixed-citation></ref><ref id="scirp.63944-ref36"><label>36</label><mixed-citation publication-type="other" xlink:type="simple">Atanasova, V., Fulcrand, H., Cheynier, V. and Moutounet, M. (2002) Effect of Oxygenation on Polyphenol Changes Occurring in the Course of Wine-Making. Analytica Chimica Acta, 458, 15-27. &lt;/br&gt;http://dx.doi.org/10.1016/S0003-2670(01)01617-8</mixed-citation></ref><ref id="scirp.63944-ref37"><label>37</label><mixed-citation publication-type="other" xlink:type="simple">Castellari, M., Matricardi, L., Arfelli, G., Galassi, S. and Amanti, A. (2000) Level of Single Bioactive Phenolics in Red Wine as a Function of the Oxygen Supplied during Storage. Food Chemistry, 69, 61-67. &lt;/br&gt;http://dx.doi.org/10.1016/S0308-8146(99)00240-X</mixed-citation></ref><ref id="scirp.63944-ref38"><label>38</label><mixed-citation publication-type="journal" xlink:type="simple"><name name-style="western"><surname>Jurd</surname><given-names> L. </given-names></name>,<etal>et al</etal>. (<year>1969</year>)<article-title>Review of Polyphenol Condensation Reactions and Their Possible Occurrence in the Aging of Wines</article-title><source> American Journal of Enology and Viticulture</source><volume> 20</volume>,<fpage> 191</fpage>-<lpage>195</lpage>.<pub-id pub-id-type="doi"></pub-id></mixed-citation></ref><ref id="scirp.63944-ref39"><label>39</label><mixed-citation publication-type="other" xlink:type="simple">Somers, T.C. (1971) The Polymeric Nature of Wine Pigments. Phytochemistry, 10, 2175-2186. &lt;/br&gt;http://dx.doi.org/10.1016/S0031-9422(00)97215-7</mixed-citation></ref><ref id="scirp.63944-ref40"><label>40</label><mixed-citation publication-type="other" xlink:type="simple">Timberlake, C.F. and Bridle, P. (1976) Interactions between Anthocyanins, Phenolic Compounds, and Acetaldehyde and Their Significance in Red Wines. American Journal of Enology and Viticulture, 27, 97-105.</mixed-citation></ref><ref id="scirp.63944-ref41"><label>41</label><mixed-citation publication-type="other" xlink:type="simple">Wang, H., Race, E.J. and Shrikhande, A.J. (2003) Anthocyanin Transformation in Cabernet Sauvignon Wine during Aging. Journal of Agricultural and Food Chemistry, 51, 7989-7994. &lt;/br&gt;http://dx.doi.org/10.1021/jf034501q</mixed-citation></ref><ref id="scirp.63944-ref42"><label>42</label><mixed-citation publication-type="other" xlink:type="simple">Klenar, I., Berovic, M. and Wondra, M. (2004) Phenolic Compounds from the Fermentation of Cultivars Cabernet Sauvignon and Merlot from the Slovenian Coastal Region. Food Technology and Biotechnology, 42, 11-17.</mixed-citation></ref><ref id="scirp.63944-ref43"><label>43</label><mixed-citation publication-type="other" xlink:type="simple">Lumuela-Raventos, R.M. and Waterhouse, A.L. (1994) A Direct HPLC Separation of Wine Phenolics. American Journal of Enology and Viticulture, 45, 1-5.</mixed-citation></ref><ref id="scirp.63944-ref44"><label>44</label><mixed-citation publication-type="other" xlink:type="simple">Nagel, C.W. and Wulf, L.W. (1979) Changes in the Antocyanins, Flavonoid and Hydroxycinnamic Acid Esters during Fermentation and Aging of Merlot and Cabernet Sauvignon. American Journal of Enology and Viticulture, 30, 111-116.</mixed-citation></ref><ref id="scirp.63944-ref45"><label>45</label><mixed-citation publication-type="other" xlink:type="simple">Oszmianski, J., Ramos, T. and Bourzeix, M. (1988) Fractionation of Phenolic Compounds in Red Wine. American Journal of Enology and Viticulture, 39, 259-262.</mixed-citation></ref><ref id="scirp.63944-ref46"><label>46</label><mixed-citation publication-type="other" xlink:type="simple">Salagoity-Auguste, M.H. and Bertrand, A. (1984) Wine Phenolics-Analysis of Low Molecular Weight Components by High Performance Liquid Chromatography. Journal of Agricultural and Food Chemistry, 35, 1241-1247. &lt;/br&gt;http://dx.doi.org/10.1002/jsfa.2740351116</mixed-citation></ref><ref id="scirp.63944-ref47"><label>47</label><mixed-citation publication-type="other" xlink:type="simple">Arnous, A., Makris, D.P. and Kefalas, P. (2002) Correlation of Pigment and Flavanol Content with Antioxidant Properties in Selected Aged Regional Wines from Greece. Journal of Food Composition and Analysis, 15, 655-665. &lt;/br&gt;http://dx.doi.org/10.1006/jfca.2002.1070</mixed-citation></ref><ref id="scirp.63944-ref48"><label>48</label><mixed-citation publication-type="other" xlink:type="simple">Monagas, M., Suarez, R., Gomez-Cordoves, C. and Bartolome, B. (2005) Simultaneous Determination of Nonanthocyanin Phenolic Compounds in Red Wines by HPLC-DAD/ESI-MS. American Journal of Enology and Viticulture, 56, 139-147.</mixed-citation></ref><ref id="scirp.63944-ref49"><label>49</label><mixed-citation publication-type="other" xlink:type="simple">Kallithraka, S., Tsoutsouras, E., Tzourou, E. and Lanaridis, P. (2006) Principal Phenolic Compounds in Greek Red Wines. Food Chemistry, 99, 784-793. &lt;/br&gt;http://dx.doi.org/10.1016/j.foodchem.2005.07.059</mixed-citation></ref><ref id="scirp.63944-ref50"><label>50</label><mixed-citation publication-type="other" xlink:type="simple">Simonetti, P., Bardana, C. and Pietta, P. (2001) Plasma Levels of Caffeic Acid and Antioxidant Status after Red Wine Intake. Journal of Agricultural and Food Chemistry, 49, 5964-5968. &lt;/br&gt;http://dx.doi.org/10.1021/jf010546k</mixed-citation></ref><ref id="scirp.63944-ref51"><label>51</label><mixed-citation publication-type="other" xlink:type="simple">Nikfardjam, M.S.P., Mark, L., Avar, P., Figler, M. and Ohmacht, R. (2006) Polyphenols, Anthocyanins and trans- Resveratrol in Red Wines from the Hungarian Villany Region. Food Chemistry, 98, 453-462. &lt;/br&gt;http://dx.doi.org/10.1016/j.foodchem.2005.06.014</mixed-citation></ref><ref id="scirp.63944-ref52"><label>52</label><mixed-citation publication-type="other" xlink:type="simple">Kallithraka, S., Arvanitoyannis, I.S., Kefalas, P., El-Zajouli, A., Soufleros, E. and Psarra, E. (2001) Instrumental and Sensory Analysis of Greek Wines Implementation of Principal Component Analysis (PCA) for Classification According to Geographical Origin. Food Chemistry, 73, 501-514.&lt;/br&gt;http://dx.doi.org/10.1016/S0308-8146(00)00327-7</mixed-citation></ref><ref id="scirp.63944-ref53"><label>53</label><mixed-citation publication-type="other" xlink:type="simple">Goldberg, D.M., Karumanchiri, A., Tsang, E. and Soleas, G.J. (1998) Catechin and Epicathechin Concentrations of Red Wines: Regional and Cultivar-Related Differences. American Journal of Enology and Viticulture, 49, 23-34.</mixed-citation></ref><ref id="scirp.63944-ref54"><label>54</label><mixed-citation publication-type="other" xlink:type="simple">Gutiérrez, I.H., Sanchez-Palomo, L.E. and Espinosa, A.V. (2005) Phenolic Composition and Magnitude of Copigmentation in Young and Shortly Aged Red Wines Made from the Cultivars, Cabernet Sauvignon, Cencibel, and Syrah. Food Chemistry, 92, 269-283. &lt;/br&gt;http://dx.doi.org/10.1016/j.foodchem.2004.07.023</mixed-citation></ref><ref id="scirp.63944-ref55"><label>55</label><mixed-citation publication-type="other" xlink:type="simple">Louw, A. (2001) The Occurrence of Bitterness in Wine: An Overview. Wynboer Technical Articles, Wynboer Magazine.</mixed-citation></ref><ref id="scirp.63944-ref56"><label>56</label><mixed-citation publication-type="other" xlink:type="simple">Proestos, C., Bakogiannis, A., Psarianos, K., Koutinas, A., Kanellaki, M. and Komaitis, M. (2005) High Performance Liquid Chromatography Analysis of Phenolic Substances in Greek Wines. Food Control, 16, 319-323. &lt;/br&gt;http://dx.doi.org/10.1016/j.foodcont.2004.03.011</mixed-citation></ref><ref id="scirp.63944-ref57"><label>57</label><mixed-citation publication-type="other" xlink:type="simple">Karagiannis, S., Economou, A. and Lanaridis, P. (2000) Phenolic and Volatile Composition of Wines Made from Vitis vinifera cv. Muscat Lefko from the Island of Samos. Journal of Agricultural and Food Chemistry, 48, 5369-5375. &lt;/br&gt;http://dx.doi.org/10.1021/jf000459c</mixed-citation></ref><ref id="scirp.63944-ref58"><label>58</label><mixed-citation publication-type="other" xlink:type="simple">Stockley, C.S. and Hoj, P.B. (2005) Better Wine for Better Health-Fact or Fiction? Australian Journal of Grape and Wine Research, 11, 127-138. &lt;/br&gt;http://dx.doi.org/10.1111/j.1755-0238.2005.tb00284.x</mixed-citation></ref><ref id="scirp.63944-ref59"><label>59</label><mixed-citation publication-type="other" xlink:type="simple">Ritchey, J.G. and Waterhouse, A.L. (1999) A Standard Red Wine: Monomeric Phenolic Analysis of Commercial Cabernet Sauvignon Wines. American Journal of Enology and Viticulture, 50, 91-100.</mixed-citation></ref><ref id="scirp.63944-ref60"><label>60</label><mixed-citation publication-type="other" xlink:type="simple">Ibern-Gomez, M., Andres-Lacueva, C., Lamuela-Raventos, R.M. and Waterhouse, A.L. (2002) Rapid HPLC Analysis of Phenolic Compounds in Red Wines. American Journal of Enology and Viticulture, 53, 218-221.</mixed-citation></ref><ref id="scirp.63944-ref61"><label>61</label><mixed-citation publication-type="other" xlink:type="simple">López, M., Martínez Del Valle, C., Orte, C. and Miró, M. (2001) Analysis of Phenolic Constituents of Biological Interest in Red Wines by High-Performance Liquid Chromatography. Journal of Chromatography A, 922, 359-363. &lt;/br&gt;http://dx.doi.org/10.1016/S0021-9673(01)00913-X</mixed-citation></ref><ref id="scirp.63944-ref62"><label>62</label><mixed-citation publication-type="other" xlink:type="simple">Soleas, G.J., Dam, J., Carey, M. and Goldberg, D.M. (1997) Toward the Fingerprint of Wines: Cultivar-Related Patterns of Polyphenolic Constituents in Ontario Wines. Journal of Agricultural and Food Chemistry, 45, 3871-3880. &lt;/br&gt;http://dx.doi.org/10.1021/jf970183h</mixed-citation></ref></ref-list></back></article>