The genus Solenopsis Westwood 1840 is known in Brazil as formiga-lava-pé, or simply as lava-pés or formiga-de-fogo (fire ant). This genus includes approximately 216 species, of which 108 occur in the New World [1] [2] . The species are omnivorous and opportunistic, feeding on insects and other alive or dead small invertebrates, as well as on plant matter [3] .

Native from South America, Solenopsis invicta Buren (1972) was accidentally introduced in many countries and has become a great public concern, causing serious damage to crops, livestock, native biodiversity, and human health [4] -[7] . The potential global range expansion of S. invicta has been correlated with temperature and precipitation, and abrupt variations of these factors may limit the success of the expansion [4] .

There are two known forms of social organization in S. invicta: monogyne and polygyne. Monogyne social form is headed by single egg-laying queens, whereas polygyne social form contains multiple egg-laying queens [8] . Polygyne colonies have a greater impact than the monogyne colonies because they have high population densities and consume a large amount of organic matter [9] .

Analyzing the genetic profile of native or introduced populations may assist in the understanding and planning of more effective control strategies. Introduced populations of potentially invasive ants show marked differences in their biology compared with native populations, such as changes in behavior, social organization, reproductive biology, and genetic profile [10] .

The intracolony genetic profile is seldom explored because genetic analyses of microsatellite markers in the genus Solenopsis using few individuals (between 1 and 10) per colony [11] -[13] may hide internal diversity and influence conclusions regarding the species’ social and reproductive dynamics. In many cases, these studies require individual analyses of worker ants from the same colony [14] .

The objective of this study was to determine the intracolony allele diversity of S. invicta by analyzing eight microsatellite loci. The bioecology of this species is well studied, but genetic information is still scarce in the areas of natural occurrence.

The S. invicta colony was collected in the central region of the city of São Paulo, São Paulo state, Brazil (23˚33'37.18"S; 46˚42'48.66"W), and the worker ants were subjected to DNA barcoding [15] -[17] . Social condition was investigated using two sets of primers described by Valles and Porter [18] -26BS (5’- CTCGCCGATTCTAACGAAGGA-3’), 16BAS (5’-ATGTATACTTTAAAGCATTCCTAATATTTTGTC-3’), 24bS (5’-TGGAGCTGATTATGATGAAGAGAAAATA-3’) and 25bAS (5’- GCTGTTTTTAATTGCATTTCTTATGCAG-3’); and Ross et al. [12] -Gp-9.for (5’- GGAGCTGATTATGATGAAGAGAAAAT-3’), Gp-9.rev (5’-AGCACAGCTTCAGCTGCTAAGA-3’), all_b.rev (5’-AGCACAGCTTCAGCTGCTAAGAT-3’) and all_b.for (5’-GGAGCTGATTATGATGAAGAGAAAATA- 3’). Eight microsatellite loci were analysed in 30 ants using the Multiplex system with primers described by Krieger and Keller [19] . Fluorescence labeling was manufactured by Applied Biosystems TM. Samples were genotyped by capillary electrophoresis in an ABI 3130 automatic sequencer (Applied Biosystems TM) and analyzed using the Genemapper^® application (Applied Biosystems TM). The alleles of the most polymorphic locus were cloned into competent bacteria and subsequently sequenced for sequence confirmation and stutter occurrence verification.

Sequencing showed that the mtDNA of ten analyzed worker ants showed 99% similarity with S. invicta haplotype 41 deposited in GenBank (access number AY950736.1). Amplification with primers for social condition showed that the colony is monogyne and that the number of microsatellite alleles varied from one to seven for the eight loci (Table 1).

Alleles in the most polymorphic locus (Sol 55) were sequenced and compared with the sequence deposited in GenBank (AF002236.1) (Figure 1). The genotyping error was plus or minus one base pair. The sequences and respective alleles, together with the GenBank accession number, are as follows: sequence 146-genotyped as allele 147 (KP734174); sequence 150-genotyped as allele 149 (KP734172); sequence 152-genotyped as allele 153 (KP734171); sequence 154-genotyped as allele 155 (KP734173), sequence 158-genotyped as allele 157 (KP734169); sequence 160-genotyped as allele 161 (KP734168); and sequence 164-genotyped as allele 163 (KP734170). An additional allele was recorded during genotyping; this allele has the same primer sequences but

a higher number of dinucleotide repeats (TC). This sequence, 166 (KP734175), is highlighted in red in Figure 1.

The number of alleles described in six of the eight loci analyzed was higher than expected for a haplodiploid crossing in a monogyne nest. The high number of alleles in the loci may be explained by a crossing between a queen ant with several males [20] . This situation has been observed only in introduced populations, and the first report was in South America, a region of natural occurrence. However, there are other possibilities: 1) the primers used were not efficient in detecting social condition, see [8] ; 2) the colony likely has more than one queen ant; or 3) the only mtDNA lineage found among the worker ants investigated may be evidence of a young nest founded by pleometrosis, with the queen ants still cooperating [21] .

Allele 166 may have been considered a stutter, and therefore, it does not appear in genotyping. Alleles 164 and 166 differ by only two bases (C and T); it is possible that the source of allele 166 is associated with a replication error or other genetic mechanisms such as an insertion [22] . Consequently, the frequency determined was low, which hampered correct identification through fluorescence labeling.

The results of this study show that thorough colony analyses may reveal characteristics not explored in population analyses. The S. invicta populations reported in Brazil are considered to be evolutionarily and regionally independent and, possibly, many social behavior characteristics of native populations are unknown [23] . Increasing our knowledge of the diversity of the colony’s social and genetic mechanisms may contribute to the development of control plans for this and other potentially invasive species. Moreover, these results may function as a warning or may alert researchers to the use of higher numbers of specimens in new studies of social insects to determine actual diversity.

Rodrigo Fernando deSouza,Fernando KamimuraCocchi,CíntiaMartins,Maria Santina de CastroMorini,Odair CorreaBueno, (2016) Characterization of Allele Diversity in a Microsatellite Locus: A Registry for Solenopsis invicta. Advances in Entomology,04,32-36. doi: 10.4236/ae.2016.41005