<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">AS</journal-id><journal-title-group><journal-title>Agricultural Sciences</journal-title></journal-title-group><issn pub-type="epub">2156-8553</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/as.2015.612142</article-id><article-id pub-id-type="publisher-id">AS-62176</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biomedical&amp;Life Sciences</subject><subject> Earth&amp;Environmental Sciences</subject></subj-group></article-categories><title-group><article-title>
 
 
  &lt;i&gt;Phaseolus&lt;/i&gt; Genetic Resources Grown in Campeche, Mexico
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>ermín</surname><given-names>Orona-Castro</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Rogelio</surname><given-names>Lépiz-Ildelfonso</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Juan</surname><given-names>Medina-Mendez</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Mónica</surname><given-names>Guadalupe Lozano-Contreras</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Mirna</surname><given-names>Hernández Pérez</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib></contrib-group><aff id="aff2"><addr-line>Universidad de Guadalajara, Guadalajara, México</addr-line></aff><aff id="aff3"><addr-line>Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Mocochá, Km 25 Carretera Mérida-Motul, Yucatán, México</addr-line></aff><aff id="aff1"><addr-line>Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Edzná, Km 15.5 Carretera Campeche-Tixmucuy, Campeche, México</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>orona.fermin@inifap.gob.mx(EO)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>10</day><month>12</month><year>2015</year></pub-date><volume>06</volume><issue>12</issue><fpage>1464</fpage><lpage>1471</lpage><history><date date-type="received"><day>18</day>	<month>November</month>	<year>2015</year></date><date date-type="rev-recd"><day>accepted</day>	<month>21</month>	<year>December</year>	</date><date date-type="accepted"><day>24</day>	<month>December</month>	<year>2015</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
  The protein intake of the population in the Yucatan Peninsula is based upon the different forms of beans naturally developed. The different ecotypes of creole beans that are grown traditionally have been displaced by improved varieties, thereby turning into a risk of losing a valuable source of germ-plasma. The present study is aimed to identify, collect and preserve several bean species genetic resources grown in Campeche, Mexico, in addition to the development of an identification card with the main phenotypic characteristics and yield components. A total of 33 different samples were collected from nine different municipalities statewide; out of those, 15 were 
  <em>Phaseolus vulgaris</em> and 18 
  <em>Phaseolus lunatus</em>. The collected samples diversity is reflected in the range of growth habits from the bush to the indeterminate climbing species, being these the most representative with about 29 samples, many of those because of the way the planting is carried out, which is traditionally performed in association with the cultivation of corn. Early samples were identified in this diversity with their cycle ranging from 60 to 120 days after emergence. Samples of 
  <em>Phaseolus lunatus</em> with 1.35 to 2.86 t/ha yield potential were also obtained.
 
</p></abstract><kwd-group><kwd>Creole Beans</kwd><kwd> Collect</kwd><kwd> Preserv</kwd><kwd> Genetic Variability</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>The bean has had a rather long traditional consumption dating all the way back to pre-Hispanic times, presenting a wide diversity for both wild and cultivated varieties [<xref ref-type="bibr" rid="scirp.62176-ref1">1</xref>] . Along the Yucatan peninsula, protein diet is based on beans naturally grown since the pre-Columbian era, differing in size (medium, small and large), shape (flat, round and kidney-shaped), plus color (white, black, brown, yellow, cream, purple, etc.). They represent valuable genetic resources with a high potential for the security of the regional food intake, providing a relevant contribution to reduce poverty due to their nutritional resource availability in underserved rural areas [<xref ref-type="bibr" rid="scirp.62176-ref2">2</xref>] [<xref ref-type="bibr" rid="scirp.62176-ref3">3</xref>] .</p><p>Different bean varieties are cultivated along the state of Campeche; however, it is threatened the conservation of seeds. Due to changes in land use by livestock, use of agrochemicals, genetic erosion, population explosion, disaster, fire and release of improved varieties have resulted in the reduction of the genetic base and the gradual disappearance of native crops or wild [<xref ref-type="bibr" rid="scirp.62176-ref4">4</xref>] .</p><p>When we lose the genetic diversity of a species and only a few copies of collection, the work of thousands of years of evolution is lost [<xref ref-type="bibr" rid="scirp.62176-ref5">5</xref>] , as in the case of populations of wild beans (Phaseolus vulgaris L.) which are used by incipiently humans, nowadays. Many of those are tolerant to stress-inducing environments; moreover, their nutritional characteristics and quality may be exceptional, representing an untapped resource, thus [<xref ref-type="bibr" rid="scirp.62176-ref6">6</xref>] .</p><p>The project “Food legumes inventory and conservation grown in the state of Campeche” was carried out with looking forward to maintaining the different bean varieties grown along the state of Campeche, throughout a plan aimed to encourage both the collecting and preservation of different edible bean variants, in several diverse species that are quite relevant for human nutrition. Consequently, the study was aimed to 1) characterize the germ-plasm richness, abundance and distribution of the Phaseolus wild and cultivated bean species; 2) develop passport identification with phenotypic characteristics and yield components; 3) preserve the genetic resources of several bean species grown in Campeche, Mexico.</p></sec><sec id="s2"><title>2. Materials and Methods</title><sec id="s2_1"><title>2.1. Collections</title><p>Samples were collected between 2011 (10 samples were lunatus and 8 vulgaris) and 2012 (8 samples lunatus and 7 vulgaris). The town and municipality of collection were determined based upon several interviews with producers, looking forward to identify and trace the collection route were wild beans were traditionally grown in the state of Campeche, performing collections in the nine municipalities of Hecelchac&#225;n, Hopelch&#233;n, Tenabo, Campeche, Champot&#243;n, Calakmul, Esc&#225;rcega, Candelaria and Carmen, plus along 39 towns (<xref ref-type="fig" rid="fig1">Figure 1</xref>).</p></sec><sec id="s2_2"><title>2.2. Establishment and Crop Handling</title><p>All of the collected samples were established on the field in the town of San Antonio Cayal, located in the Campeche municipality (19˚45'08.14&quot;N and 90˚09'51.84&quot;W, ASNM 31 m); these were sown in plots of six rows and six meters long; the planting distance between rows was 80 cm, plus 10 cm in between plants; a 50 kg density of seed per hectare was used. The cultivation conditions were under temporary planting, having August 24th, 2012 set as the cultivation date. The harvesting took place at the beginning of November for the early samples, coming to an end in the same year by December 29th with the late genotypes. The type of soil in this region is Luvisols known as K’ankab (classification according to the Mayan), red soils with high clay content but with permeable drainage [<xref ref-type="bibr" rid="scirp.62176-ref7">7</xref>] .</p><p>The agricultural practice consisted on preparing the cultivation soil throughout two dragging phases, performing the second by cross; the trenching was then carried out, followed by the drawing of the plot size; the planting was done, afterwards. A basal fertilizer application was performed using ammonium phosphate in dosages of 80 kg per hectare, plus 30 kg of nitrogen per hectare. Both manual and chemical weed control, as well as pest control was performed; furthermore, tutors were also set on site because most of the Phaseolus collected were samples sown associated with corn and classified as type IV growth.</p><p>The data was recorded in order to differentiate the samples as flowering days, maturity days, grain color, flower color, grains number per pod, grain yield and growth habit; the bean evaluation manual was used for data evaluation [<xref ref-type="bibr" rid="scirp.62176-ref8">8</xref>] .</p></sec></sec><sec id="s3"><title>3. Results and Discussion</title><p>A total of 33 Phaseolus samples were collected; 15 samples were from the vulgaris species and 18 were lunatus</p><fig id="fig1"  position="float"><label><xref ref-type="fig" rid="fig1">Figure 1</xref></label><caption><title> Routes traveled for the collection of wild Phaseolus species in the state of Campeche</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/6-3001305x6.png"/></fig><p>(<xref ref-type="table" rid="table1">Table 1</xref>). Four variants of the genotype locally known as the T’zam&#225;a were identified in the sampling collection from the Hecelchacan, Hopelchen and Champot&#243;n municipalities (red sheath, purple short sheath and the last being long marbled sheath); the first two plus the last belong to the type IV or beans that require a tutor guide for their proper development (<xref ref-type="fig" rid="fig2">Figure 2</xref>); two Mejen buul variants were also found at the towns of Chencoh and Iturbide, in the Hopelchen municipality, one of short sheath and another of long. An early lunatus (60 days maturity) was found at the town of Dzitnup, in the Hecelchac&#225;n municipality; moreover, late lunatus samples (132 days maturity) were also found at the town of San Antonio Cayal, in the Campeche municipality.</p><sec id="s3_1"><title>3.1. Genotype Geographical Distribution</title><p>The largest number of Phaseolus genotypes were located in the Campeche municipality (21 genotypes), followed by the Champot&#243;n and Hecelchac&#225;n with 4 genotypes, respectively. The lowest number of genotypes was located in the municipalities of Hopelch&#233;n (2), Tenabo (1) and Esc&#225;rcega (1) (<xref ref-type="fig" rid="fig3">Figure 3</xref>). It is noteworthy that in the municipalities that were not reported in <xref ref-type="fig" rid="fig3">Figure 3</xref> (Tenabo, Calakmul, Candelaria and Carmen), the Phaseolus collection was performed; nevertheless, similarities among the Phaseolus species collected per town were determined during the evaluation of the field. The evaluation of the collected genetic variability was performed based upon the expression of the different morphological characteristics in each accession related to the plant, flower, sheath and grain vegetative growth. These characteristics were repeated in several accessions.</p><p>The lunatus species is recognized by the Mayan farmers as ib, ibes or lime bean; the cultivation of this bean in the region has played a key role in the diet of the Mayan culture, being the fourth most important crop for the traditional Mayan agriculture in the Yucatan peninsula [<xref ref-type="bibr" rid="scirp.62176-ref9">9</xref>] -[<xref ref-type="bibr" rid="scirp.62176-ref12">12</xref>] . Consequently, it is understood that many of the collected samples during harvesting belong to the lunatus species (<xref ref-type="fig" rid="fig4">Figure 4</xref>), being the Campeche municipality where the largest sample of this species were found (12 collections), compared to the vulgaris species (9 collections).</p><table-wrap id="table1" ><label><xref ref-type="table" rid="table1">Table 1</xref></label><caption><title> Origin, common name and characteristics of 33 bean populations collected in the state of Campeche during 2011 and 2012</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Col.</th><th align="center" valign="middle" >Located</th><th align="center" valign="middle" >Common name</th><th align="center" valign="middle" >Beans color</th><th align="center" valign="middle" >Days to flowering</th><th align="center" valign="middle" >Days to maturity</th><th align="center" valign="middle" >Weight of 500 seeds (g)</th><th align="center" valign="middle" >Production cycle</th></tr></thead><tr><td align="center" valign="middle" >C1</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >T’zam&#225;a red sheath</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >40</td><td align="center" valign="middle" >113</td><td align="center" valign="middle" >98.94</td><td align="center" valign="middle" >*Intermediate</td></tr><tr><td align="center" valign="middle" >C2</td><td align="center" valign="middle" >Hopelch&#233;n</td><td align="center" valign="middle" >Cole buul</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >38</td><td align="center" valign="middle" >115</td><td align="center" valign="middle" >103.38</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C3</td><td align="center" valign="middle" >Hecelchac&#225;n</td><td align="center" valign="middle" >Mejen ib</td><td align="center" valign="middle" >Red</td><td align="center" valign="middle" >30</td><td align="center" valign="middle" >70</td><td align="center" valign="middle" >127.44</td><td align="center" valign="middle" >Short</td></tr><tr><td align="center" valign="middle" >C4</td><td align="center" valign="middle" >Hecelchac&#225;n</td><td align="center" valign="middle" >Ibes white</td><td align="center" valign="middle" >White</td><td align="center" valign="middle" >50</td><td align="center" valign="middle" >132</td><td align="center" valign="middle" >132.22</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C5</td><td align="center" valign="middle" >Hopelch&#233;n</td><td align="center" valign="middle" >Ibes munition</td><td align="center" valign="middle" >White</td><td align="center" valign="middle" >42</td><td align="center" valign="middle" >112</td><td align="center" valign="middle" >142.62</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C6</td><td align="center" valign="middle" >Hecelchac&#225;n</td><td align="center" valign="middle" >Zot chet buul</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >35</td><td align="center" valign="middle" >80</td><td align="center" valign="middle" >112.5</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C7</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Bush black beans</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >42</td><td align="center" valign="middle" >118</td><td align="center" valign="middle" >75.03</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C8</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Colored green beans</td><td align="center" valign="middle" >Red</td><td align="center" valign="middle" >49</td><td align="center" valign="middle" >132</td><td align="center" valign="middle" >89.04</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C9</td><td align="center" valign="middle" >Champot&#243;n</td><td align="center" valign="middle" >Red creole beans</td><td align="center" valign="middle" >Red</td><td align="center" valign="middle" >47</td><td align="center" valign="middle" >129</td><td align="center" valign="middle" >110.47</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C10</td><td align="center" valign="middle" >Champot&#243;n</td><td align="center" valign="middle" >Mejen buul</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >32</td><td align="center" valign="middle" >73</td><td align="center" valign="middle" >145.16</td><td align="center" valign="middle" >Short</td></tr><tr><td align="center" valign="middle" >C11</td><td align="center" valign="middle" >Champot&#243;n</td><td align="center" valign="middle" >Rod bean red sheath</td><td align="center" valign="middle" >Red</td><td align="center" valign="middle" >43</td><td align="center" valign="middle" >120</td><td align="center" valign="middle" >129.95</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C12</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >T’zam&#225;a green sheath</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >41</td><td align="center" valign="middle" >113</td><td align="center" valign="middle" >141.32</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C13</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >T’zam&#225;a Bush long sheath</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >35</td><td align="center" valign="middle" >85</td><td align="center" valign="middle" >131.5</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C14</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >T’zam&#225;a marbled sheath</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >42</td><td align="center" valign="middle" >115</td><td align="center" valign="middle" >120.89</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C15</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes pinto creole</td><td align="center" valign="middle" >Pinto</td><td align="center" valign="middle" >45</td><td align="center" valign="middle" >110</td><td align="center" valign="middle" >193.31</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C16</td><td align="center" valign="middle" >Hecelchac&#225;n</td><td align="center" valign="middle" >Ibes white plane</td><td align="center" valign="middle" >White</td><td align="center" valign="middle" >45</td><td align="center" valign="middle" >115</td><td align="center" valign="middle" >139.31</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C17</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Charro beans</td><td align="center" valign="middle" >Red</td><td align="center" valign="middle" >35</td><td align="center" valign="middle" >102</td><td align="center" valign="middle" >282.17</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C18</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes cream plane</td><td align="center" valign="middle" >Cream</td><td align="center" valign="middle" >43</td><td align="center" valign="middle" >113</td><td align="center" valign="middle" >136.97</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C19</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes dark purple</td><td align="center" valign="middle" >Dark purple</td><td align="center" valign="middle" >41</td><td align="center" valign="middle" >119</td><td align="center" valign="middle" >175.99</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C20</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes light red</td><td align="center" valign="middle" >Red</td><td align="center" valign="middle" >35</td><td align="center" valign="middle" >95</td><td align="center" valign="middle" >136.76</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C21</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes light purple</td><td align="center" valign="middle" >Light purple</td><td align="center" valign="middle" >35</td><td align="center" valign="middle" >95</td><td align="center" valign="middle" >167.21</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C22</td><td align="center" valign="middle" >Esc&#225;rcega</td><td align="center" valign="middle" >Ibes black plane</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >42</td><td align="center" valign="middle" >112</td><td align="center" valign="middle" >177.67</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C23</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Creole Bush beans</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >30</td><td align="center" valign="middle" >73</td><td align="center" valign="middle" >87.07</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C24</td><td align="center" valign="middle" >Tenabo</td><td align="center" valign="middle" >Ibes pink</td><td align="center" valign="middle" >Pink</td><td align="center" valign="middle" >30</td><td align="center" valign="middle" >90</td><td align="center" valign="middle" >132.62</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C25</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes light pink</td><td align="center" valign="middle" >Pink</td><td align="center" valign="middle" >45</td><td align="center" valign="middle" >115</td><td align="center" valign="middle" >140.21</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C26</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes cream-black pinto</td><td align="center" valign="middle" >Cream-Black Pinto</td><td align="center" valign="middle" >45</td><td align="center" valign="middle" >115</td><td align="center" valign="middle" >149.87</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C27</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes white-black pinto</td><td align="center" valign="middle" >White-Black Pinto</td><td align="center" valign="middle" >42</td><td align="center" valign="middle" >112</td><td align="center" valign="middle" >170.0</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C28</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes black</td><td align="center" valign="middle" >Black</td><td align="center" valign="middle" >42</td><td align="center" valign="middle" >112</td><td align="center" valign="middle" >159.86</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C29</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes fucsia</td><td align="center" valign="middle" >Fuscia</td><td align="center" valign="middle" >30</td><td align="center" valign="middle" >90</td><td align="center" valign="middle" >133.86</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C30</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes cream-black marbled</td><td align="center" valign="middle" >Cream-Black marbled</td><td align="center" valign="middle" >43</td><td align="center" valign="middle" >113</td><td align="center" valign="middle" >165.28</td><td align="center" valign="middle" >Intermediate</td></tr><tr><td align="center" valign="middle" >C31</td><td align="center" valign="middle" >Kest&#233;</td><td align="center" valign="middle" >Bean pigeon paw</td><td align="center" valign="middle" >Red</td><td align="center" valign="middle" >48</td><td align="center" valign="middle" >131</td><td align="center" valign="middle" >45.18</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C32</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Ibes cream-pink pinto</td><td align="center" valign="middle" >Cream-Pink Pinto</td><td align="center" valign="middle" >45</td><td align="center" valign="middle" >118</td><td align="center" valign="middle" >141.45</td><td align="center" valign="middle" >Long</td></tr><tr><td align="center" valign="middle" >C33</td><td align="center" valign="middle" >Campeche</td><td align="center" valign="middle" >Red beans</td><td align="center" valign="middle" >Red</td><td align="center" valign="middle" >45</td><td align="center" valign="middle" >118</td><td align="center" valign="middle" >85.38</td><td align="center" valign="middle" >Intermediate</td></tr></tbody></table></table-wrap><p>*Production cycle times: Short (60 - 90 days), Intermediate (91 - 120 days), Long (121 - 150 days)</p><fig-group id="fig2"><label><xref ref-type="fig" rid="fig2">Figure 2</xref></label><caption><title> Grain and bean plants native Phaseolus vulgaris variety T'zam&#225;a (red sheath) collected in the municipality Campeche.</title></caption><fig id ="fig2_1"><label></label><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/6-3001305x7.png"/></fig><fig id ="fig2_2"><label></label><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/6-3001305x8.png"/></fig></fig-group><fig id="fig3"  position="float"><label><xref ref-type="fig" rid="fig3">Figure 3</xref></label><caption><title> List of collections per municipality in the state of Campeche, Mexico</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/6-3001305x9.png"/></fig><fig id="fig4"  position="float"><label><xref ref-type="fig" rid="fig4">Figure 4</xref></label><caption><title> Phaseolus species collected by municipalities</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/6-3001305x10.png"/></fig><p>A pretty good idea related with the relevance of genetic resources is provided by such bean diversity among regional farmers, as well as the in situ germ-plasma preservation, which keeps being used year after year as part of the family heritage [<xref ref-type="bibr" rid="scirp.62176-ref13">13</xref>] . Features such as flavor, cooking quality, yield stability rather than local adaptability and performance are important attributes that lead farmers to preserve their “wild” varieties, keeping the genetic wealth of their communities [<xref ref-type="bibr" rid="scirp.62176-ref14">14</xref>] .</p></sec><sec id="s3_2"><title>3.2. Collected Genetic Variability Preservation and Usage</title><p>An objective of this sample collection was to have the genetic variation revealed in the traditional ecotypes for their future preservation and subsequent improvement. The collected and evaluated samples were sent to the germ-plasma bank based at the National Plant Genetic Resources for Food and Agriculture (SINAREFI), located at the University of Guadalajara in the municipality of Zapopan, Jalisco.</p></sec><sec id="s3_3"><title>3.3. Yield</title><p>The yield achieved by the collections that were carried out is shown in <xref ref-type="table" rid="table2">Table 2</xref>, which revealed higher yields than those 615 kg/ha [<xref ref-type="bibr" rid="scirp.62176-ref15">15</xref>] reported in the state of Campeche, having five lunatus and three vulgaris species standing out. The highest yield took place in the mejen ib, the big flat black ibes and the big white ibes, with 2867, 1327 and 1282 kg/ha, respectively.</p><p>The wild varieties potential versus the commercial varieties has been reported by several studies in such regard, mainly concerning yield performance, physical quality and protein content [<xref ref-type="bibr" rid="scirp.62176-ref16">16</xref>] [<xref ref-type="bibr" rid="scirp.62176-ref17">17</xref>] . Additionally, the bean that is both produced and consumed in Mexico comes mainly from native genotypes in lower levels of improved varieties, which have agronomic and quality advantages [<xref ref-type="bibr" rid="scirp.62176-ref18">18</xref>] .</p><p>The precipitation that took place from the samples planting date (<xref ref-type="fig" rid="fig5">Figure 5</xref>), to the harvest for seed increase, was 464 mm, if taking into account that the bean water necessity ranges from 600 to 700 mm in 3 or 4 months</p><table-wrap id="table2" ><label><xref ref-type="table" rid="table2">Table 2</xref></label><caption><title> Yield collections of wild beans in the state of Campeche</title></caption><table><tbody><thead><tr><th align="center" valign="middle" >Species</th><th align="center" valign="middle" >Common name</th><th align="center" valign="middle" >Yield kg/ha</th></tr></thead><tr><td align="center" valign="middle"  rowspan="5"  >lunatus</td><td align="center" valign="middle" >Mejen Ib</td><td align="center" valign="middle" >2867</td></tr><tr><td align="center" valign="middle" >Ibes black plane</td><td align="center" valign="middle" >1327</td></tr><tr><td align="center" valign="middle" >Ibes white</td><td align="center" valign="middle" >1282</td></tr><tr><td align="center" valign="middle" >Ibes munition</td><td align="center" valign="middle" >1104</td></tr><tr><td align="center" valign="middle" >Ibes pinto creole</td><td align="center" valign="middle" >788</td></tr><tr><td align="center" valign="middle"  rowspan="4"  >vulgaris</td><td align="center" valign="middle" >T’zam&#225;a red sheath</td><td align="center" valign="middle" >953</td></tr><tr><td align="center" valign="middle" >T’zam&#225;a green sheath</td><td align="center" valign="middle" >755</td></tr><tr><td align="center" valign="middle" >T’zam&#225;a marbled sheath</td><td align="center" valign="middle" >943</td></tr><tr><td align="center" valign="middle" >Mejen buul</td><td align="center" valign="middle" >633</td></tr></tbody></table></table-wrap><fig id="fig5"  position="float"><label><xref ref-type="fig" rid="fig5">Figure 5</xref></label><caption><title> Annual precipitation in the town of Cayal in 2012. The arrow indicates the date of planting material collected for seed increase</title></caption><graphic mimetype="image"   position="float"  xlink:type="simple"  xlink:href="http://html.scirp.org/file/6-3001305x11.png"/></fig><p>[<xref ref-type="bibr" rid="scirp.62176-ref19">19</xref>] , having only 66% of the cultivation requirements covered; nonetheless, nine of the collected samples exceeded the 615 kg/ha yield average statewide (<xref ref-type="table" rid="table2">Table 2</xref>), proving their tolerance ability to extreme weather conditions. As reported by several studies, wild beans are naturally adapted to survive harsh climatic fluctuations until their final germination, under favorable conditions of subsequent cycles, occurs [<xref ref-type="bibr" rid="scirp.62176-ref20">20</xref>] -[<xref ref-type="bibr" rid="scirp.62176-ref22">22</xref>] as observed in the study hereby. The average temperature recorded during the evaluation was 26˚C, maximum was 36˚C and minimum of 18˚C, recorded at the end of the crop cycle.</p></sec></sec><sec id="s4"><title>4. Conclusion</title><p>A total of 33 different types of Phaseolus species were identified and collected in the state of Campeche, out of which 15 belonged to the P. vulgaris species and 18 to P. lunatus. The diversity of the collected samples was reflected in the range of growth habits from the bush to the indeterminate climbing species, being the latter the most representative, with about 29 samples, many of those due because of how they were planted, which was a process traditionally performed in association with the cultivation of corn. Early samples were identified in this diversity with a 60 to 120 days fluctuating cycle after emergence; samples with a 1.35 to 2.86 t/ha yield potential were obtained in two Phaseolus lunatus samples.</p></sec><sec id="s5"><title>Cite this paper</title><p>Ferm&#237;nOrona-Castro,RogelioL&#233;piz-Ildelfonso,JuanMedina-Mendez,M&#243;nica GuadalupeLozano-Contreras,MirnaHern&#225;ndez P&#233;rez, (2015) Phaseolus Genetic Resources Grown in Campeche, Mexico. Agricultural Sciences,06,1464-1471. doi: 10.4236/as.2015.612142</p></sec></body><back><ref-list><title>References</title><ref id="scirp.62176-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">Espinosa-Pérez, E.N., Ramírez-Vallejo, P., Crosby-Galván, M.M., Estrada-Gómez, J.A., Lucas-Florentino, B. and Chávez-Servia, J.L. (2015) Clasificación de poblaciones nativas de frijol común del centro-sur de México por morfología de semilla. Revista Fitotecnia Mexicana, 38, 29-38.  
http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0187-73802015000100005&amp;lng=pt&amp;tlng=es.</mixed-citation></ref><ref id="scirp.62176-ref2"><label>2</label><mixed-citation publication-type="other" xlink:type="simple">Ballesteros, P.G., Torres, G.A. and Barrera, M. (2000) Reincorporación del frijol carauta (Phaseolus lunatus L.) a la agricultura tradicional en el resguardo indígena de San Andrés de Sotavento (Córdoba, Colombia). Plant Genetic Resources Newsletter, No. 123, 23-27.  
http://www.bioversityinternational.org/fileadmin/user_upload/online_library/publications/pdfs/Plant_Genetic_Resources_Newsletter_No_123.pdf</mixed-citation></ref><ref id="scirp.62176-ref3"><label>3</label><mixed-citation publication-type="other" xlink:type="simple">Hernández-López, V.M., Vargas-Vázquez, M.L.P., Muruaga-Martínez, J.S., Hernández-Delgado, S. and Mayek-Pérez, N. (2013) Origen, domesticación y diversificación del frijol común: Avances y perspectivas. Revista fitotecnia mexicana, 36, 95-104. http://www.revistafitotecniamexicana.org/documentos/36-2/1a.pdf</mixed-citation></ref><ref id="scirp.62176-ref4"><label>4</label><mixed-citation publication-type="other" xlink:type="simple">López Soto, J.L., Ruiz Corral, J.A., Sánchez González, J.D.J. and Lépiz Ildefonso, R. (2005) Adaptación climática de 25 especies de frijol silvestre (Phaseolus spp) en la república mexicana. Revista Fitotecnia Mexicana, 28, 221-230.  
http://www.redalyc.org/articulo.oa?id=61028306</mixed-citation></ref><ref id="scirp.62176-ref5"><label>5</label><mixed-citation publication-type="book" xlink:type="simple">Rivas, M. (2001) Conservación in situ de los recursos fitogenéticos. In: Estrategia en recursos fitogenéticos para los países del Cono Sur. Ed., Programa Cooperativo para el Desarrollo Tecnológico Agroalimentario y Agroindustrial del Cono Sur, Montevideo, 63-76. http://repiica.iica.int/DOCS/B0630E/B0630E.PDF</mixed-citation></ref><ref id="scirp.62176-ref6"><label>6</label><mixed-citation publication-type="other" xlink:type="simple">Pe&amp;ntildea-Valdivia, C.B., Trejo, C., Celis-Velazquez, R. and López Ordáz, A. (2013) Reacción del frijol silvestre (Phaseolus vulgaris L.) a la profundidad de siembra. Revista mexicana de ciencias agrícolas, 4, 89-102.  
http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2007-09342013000100007&amp;lng=es&amp;tlng=es.</mixed-citation></ref><ref id="scirp.62176-ref7"><label>7</label><mixed-citation publication-type="other" xlink:type="simple">Estrada-Medina, H., Bautista, F., Jiménez-Osornio, J.J.M., González-Iturbe, J.A. and Aguilar Cordero, W.J. (2013) Maya and WRB Soil Classification in Yucatan, Mexico: Differences and Similarities. International Scholarly Research Notices Soil Science, 2013, Article ID: 634260. http://dx.doi.org/10.1155/2013/634260</mixed-citation></ref><ref id="scirp.62176-ref8"><label>8</label><mixed-citation publication-type="other" xlink:type="simple">Schoonhoven, A.V. and Pastor-Corrales, M.A. (1987) Sistema estándar para la evaluación de germoplasma de frijol. Centro Internacional de Agricultura Tropical, Cali, 56.</mixed-citation></ref><ref id="scirp.62176-ref9"><label>9</label><mixed-citation publication-type="book" xlink:type="simple">Martínez-Castillo, J., May-Pa, F., Zizumbo-Villareal, D. and García-Marín, P.C. (2004) Diversidad intraespecífica del ib (Phaseolus lunatus L.) en la agricultura tradicional de la Península de Yucatán, México. In: Chávez-Servia, J.L., Tuxill, J. and Jarvis, D.I., Eds., Manejo de la diversidad de los cultivos en los agrosistemas tradicionales, Instituto internacional de recursos fitogenéticos, Cali, 26-35.  
https://books.google.com.mx/books?hl=es&amp;lr=&amp;id=6ptxbNgluVkC&amp;oi=fnd&amp;pg=PA26&amp;dq=colecta+de+frijol+cicy&amp;ots=U5kD4ILlGm&amp;sig=xQXtjbuJ-g2ORQlA3xq846ey63o#v=onepage&amp;q&amp;f=false</mixed-citation></ref><ref id="scirp.62176-ref10"><label>10</label><mixed-citation publication-type="other" xlink:type="simple">Wicab-Cámara, G.N. and Martínez-Castillo, J. (2011) El frijol maya del siglo XXI. Revista de divulgación científica y tecnológica de la Universidad Veracruzana., 24, 7.  
http://www.uv.mx/cienciahombre/revistae/vol24num2/articulos/frijol/</mixed-citation></ref><ref id="scirp.62176-ref11"><label>11</label><mixed-citation publication-type="other" xlink:type="simple">Félix, D.-T., Coello-Coello, J. and Martínez-Castillo, J. (2014) Wild to Crop Introgression and Genetic Diversity in Lima Bean (Phaseolus lunatus L.) in Traditional Mayan Milpas from Mexico. Conservation Genetics, 15, 1315-1328. 
http://dx.doi.org/10.1007/s10592-014-0619-7</mixed-citation></ref><ref id="scirp.62176-ref12"><label>12</label><mixed-citation publication-type="other" xlink:type="simple">Martínez-Castillo, J., Camacho-Pérez, L., Coello-Coello, J. and Andueza-Noh, R. (2012) Wholesale Replacement of Lima Bean (Phaseolus lunatus L.) Landraces over the Last 30 Years in Northeastern Campeche, Mexico. Genetic Resources and Crop Evolution, 59, 191-204. http://dx.doi.org/10.1007/s10722-011-9675-8</mixed-citation></ref><ref id="scirp.62176-ref13"><label>13</label><mixed-citation publication-type="other" xlink:type="simple">Lagunes-Espinoza, L.del.C., Gallardo-López, F., Becerril-Hernández, H. and Bola&amp;ntildeos-Aguilar, E.D. (2008) Diversidad cultivada y sistema de manejo de Phaseolus vulgaris y Vigna unguiculata en la región de la Chontalpa, Tabasco. Revista Chapingo. Serie horticultura, 14, 13-21. 
http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1027-152X2008000100003&amp;lng=es&amp;tlng=es</mixed-citation></ref><ref id="scirp.62176-ref14"><label>14</label><mixed-citation publication-type="other" xlink:type="simple">Arunachalam, M. (2004) Participatory Conservation: A Means of Encouraging Community Biodiversity. PGR Newsletter, No. 122, 1-6. http://www.bioversityinternational.org/fileadmin/PGR/article-issue_122-art_1-lang_en.html</mixed-citation></ref><ref id="scirp.62176-ref15"><label>15</label><mixed-citation publication-type="other" xlink:type="simple">Servicio de Información y Estadística Agroalimentaria y Pesquera (SIAP) (2015) Sistema de Información Agropecuarias de Consulta (SIACON). Secretaria de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación. Versión 1.1, México, D.F. http://www.siap.gob.mx</mixed-citation></ref><ref id="scirp.62176-ref16"><label>16</label><mixed-citation publication-type="other" xlink:type="simple">Ayala-Garay, O.J., Pichardo-González, J.M., Estrada-Gómez, J.A., Carrillo-Salazar, J.A. and Hernández-Livera, A. (2006) Rendimiento y calidad de semilla del frijol ayocote en el Valle de México. Agricultura técnica en México, 32, 313-321. http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0568-25172006000300007&amp;lng=es&amp;tlng=pt</mixed-citation></ref><ref id="scirp.62176-ref17"><label>17</label><mixed-citation publication-type="other" xlink:type="simple">Ramírez-Pérez, A.R., Díaz-Ruiz, R., Jacinto-Hernández, C., Paredes-Sánchez, J.A. and Garza García, R. (2012) Diversidad de frijoles nativos de diferentes regiones del estado de Puebla. Revista mexicana de ciencias agrícolas, 3, 467-480. http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2007-09342012000300005&amp;lng=es&amp;tlng=es</mixed-citation></ref><ref id="scirp.62176-ref18"><label>18</label><mixed-citation publication-type="other" xlink:type="simple">Mu&amp;ntildeoz, V.E.E., Rubio, H.D., Bernal, L.I., Garza, G.R. and Jacinto, H.C. (2009) Caracterización de genotipos nativos de frijol del estado de hidalgo, con base a calidad del grano. Agricultura Técnica en México, 35, 426-435. 
http://www.scielo.org.mx/pdf/agritm/v35n4/v35n4a8.pdf</mixed-citation></ref><ref id="scirp.62176-ref19"><label>19</label><mixed-citation publication-type="other" xlink:type="simple">Villanueva, D.J., Loredo, O.C. and Hernández, R.A. (2001) Requerimientos hídricos de especies anuales y perennes en las zonas media y altiplano de San Luis Potosí. INIFAP. Campo Experimental Palma de la Cruz. Folleto Técnico, 12, 25-26.</mixed-citation></ref><ref id="scirp.62176-ref20"><label>20</label><mixed-citation publication-type="other" xlink:type="simple">Vi&amp;ntildeals, M.E., Ortiz, R., Ponce, M. and Ríos, H. (2002) Análisis de la diversidad fenotípica de variedades de frijol (P. vulgaris L.) utilizadas por los campesinos en la comunidad La Palma en Pinar del Río. Cultivos Tropicales, 23, 15-19. 
http://www.redalyc.org/articulo.oa?id=193218105002</mixed-citation></ref><ref id="scirp.62176-ref21"><label>21</label><mixed-citation publication-type="other" xlink:type="simple">Pe&amp;ntildea-Valdivia, C.B., Hernández, G.E.del.R., Bernal-Lugo, I. and Aguirre, R.J.R. (1999) Seed Quality of Wild and Domesticated Common Bean (Phaseolus vulgaris L.) after Storage. Interciencia, 24, 8-13. 
http://www.interciencia.org/v24_01/pena.pdf</mixed-citation></ref><ref id="scirp.62176-ref22"><label>22</label><mixed-citation publication-type="other" xlink:type="simple">Sánchez Urdaneta, A.B., Pe&amp;ntildea Valdivia, C.B., Trejo, C., Aguirre, R.J.R., Cárdenas, E. and Galicia Jiménez, A.B. (2003) Permeabilidad de las membranas radicales de plántulas de frijol (Phaseolus vulgaris L.) silvestre y domesticado bajo déficit de humedad. Interciencia, 28, 597-603. http://uaeh.redalyc.org/articulo.oa?id=33908507</mixed-citation></ref></ref-list></back></article>