Oil pollution is a global challenge. It adversely affects fertile land and marine ecosystems [1] [2] and ultimately the health of people around the contaminated site [3] . Pollutants are introduced into the ecosystem as a result of human activities. Used oil is disposed indiscriminately after engine services in some countries both by mechanics and individuals. These pollutants which seep into water bodies and farmland are hazardous to living things. [4] reported that large amount of hydrocarbons were found in used oil including the highly toxic Polycyclic Aromatic Hydrocarbon (PAH). The physical, chemical and microbiological contents of soil were changed and negatively affected where these were found [5] . Consequently, these polluted sites require some remedial measures for clean-up. One of such measures is bioremediation. Several studies have been conducted on the use of edible fungi in bioremediation. Among these are Pleurotus osteatus [6] [7] ; Pleurotus pulmonarius, Pleurotus squarrosulus, Pleurotus tuber regium [8] [9] ; Pleurotus tuber-regium, Lentinus subnudus [10] and Pleurotus eryngii [11] .

[8] reported the use of mushrooms in bioaugmentation in a polluted site where cowpea was planted. However, reports are limited on use of sporophores after bioremediation processes especially in relation to possible human consumption. Is oyster mushroom used for bioremediation fit for human consumption? Is it also safe to pick mushrooms from oil polluted soil if found? If the mushroom is assessed safe for consumption, it could both supplement protein intake and income of people where oil pollution is a great challenge to food security. This study was designed to evaluate the remnant hydrocarbon in the fruiting body of Pleurotus pulmonarius and to assess its safety for human consumption.

A simulation experiment was carried out in which samples were treated according to the method of [12] modified as follows: Briefly, one kilogram of sandy loam soil was weighed into transparent polythene bags. The samples were treated with 10% (v/w) Spent Engine Oil. 10% (w/w) waste cotton were added to the polluted samples (as substrate for the mushroom) and thoroughly mixed with it. The samples were sterilized in an autoclave at 121˚C for 15 minutes and inoculated with 10% Pleurotus pulmonarius. Control experiment was not treated with SEO. All the treatments were incubated at room temperature (28˚C ± 2˚C) for four weeks.

Fruiting bodies of P. pulmonarius were found growing on the polluted soil after four weeks. The total hydrocarbon contents of mushrooms harvested from the soil polluted with SEO are presented in Figure 1 and Figure 2. The total remnant Aliphatic Hydrocarbon (AH) was 23 mg/kg. The Aliphatic Hydrocabon profile indicated n- Octadecane (0.34 mg/kg), n-Phytane (0.30 mg/kg), n-Nonadecane (0.28 mg/kg), n-Eicosane (0.74 mg/kg), n- Heneicosane (1.47 mg/kg), n-Docosane (1.76 mg/kg), n-Heptacosane (1.98 mg/kg), n-Nonacosane (1.53 mg/kg), n-Tricontane (1.57 mg/kg), n-Hentriacontane (1.30 mg/kg), n-Octacosane (2.01 mg/kg), n-Hexacosane (2.19 mg/kg), n-Tricosane (2.35 mg/kg), n-Pentacosane (2.36 mg/kg) and n-Tetracosane (2.72 mg/kg) ranged between 0.28 and 2.72 mg/kg (Figure 1). Similarly, total remnant PAH was 10mg/kg. From the results of the PAH profile Naphthalene, Acenaphthene, Fluorene, Anthracene, Dibenzo (a, h) anthracene, Benzo (g, h, i) perylene and Indeno (1, 2, 3-d) pyrene were not detected (Table 1). However, Fluoranthene (0.06 mg/kg), Acenapthylene (0.22 mg/kg), 2-Methylnaphtalene (0.26 mg/kg), Phenanthrene (0.32 mg/kg), Benzo (k) fluoranthene (0.37 mg/kg), Benzo (a) anthracene (0.42 mg/kg), Benzo (b) fluoranthene (0.46 mg/kg), Benzo (a) pyrene (0.54 mg/kg) and chrysene (5.30 mg/kg) indicated a range between 0.42 and 5.30 mg/kg (Table 2).

The fruiting bodies of P. pulmonarius growing on polluted soil suggested that the fungus, with the aid of hydro-

lytic enzymes, produced, degraded the SEO thereby proliferating its own cells. [14] reported that the degradation ability of mushrooms was dependent on the enzymes they produced. Other researchers who have reported the ability of mushrooms to clean up toxic substances include [15] - [17] . The AH profile ranged from 0.28 - 1.98 mg/kg and the PAH profile ranged from 0.00 - 0.06 mg/kg were within the non-cancer dose while those with higher values were outside the non-cancer reference dose [18] .

Similar reference doses were recorded by Canadian Council of the Ministers of the Environment (CCME) [19] as the soil quality guideline for portable water. According to CCME document, PAHs are a group of complex hydrocarbons made up of two or more benzene rings fused in a linear, angular or cluster arrangement and some are carcinogenic while others are not. With the presence of remnant hydrocarbons in the analyzed mushrooms, they may not be recommended for human consumption. However, possibilities of consuming mushrooms used for bioremediation may not be impossible as reported by [15] .

The detection of some hydrocarbon profiles above the non-cancer daily reference dose makes the tested mushroom used for bioremediation not safe for human consumption. This underscores the need for caution in consuming mushrooms found in oil polluted environment. Further research is suggested probably with a longer period of incubation, use of other species of mushroom or other petroleum products.

The author wishes to appreciate the immense contributions and constructive criticisms of Dr. Kolawole Falade and Prof. O. Akaranta.