The HPLC-grade methanol was obtained from J. T. Baker Chemical Co. (Phillipsburg, NJ, USA). Tert-Butyl hydroquinone (TBHQ) was obtained from Alfa Aesar (Heysham, Lancashire, UK) and hydrochloric acid was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Sinapine was purchased from Chem. Faces (Wuhan, China) and kaempferol was purchased from Wako, Japan. Ultra-pure water used in this study was made by PURE LAB Option-Q system (ELGA Lab Water, VWS Ltd., UK).

Two types of rapeseeds cultivated in Jeju Island (Jeju local cultivar) and Muan (“Sunmang”) were annually provided from 2011 to 2013 by the Bio-Energy Crop Center, National Institute of Food Science (Muan, Korea). The Jeju local cultivar was cultivated from October to May, whereas “Sunmang” was grown from October to June. After rapeseeds were harvested and de-oiled, the residue, rapeseed meal, were provided to chemical analyzes for phenolic compositions. In 2014, imported rapeseed meal from India was obtained from the Department of Animal Resource and Science, Dankook University.

Extraction of phenolic compositions was performed following the standard method with slight modifications [10] . Rapeseed meal (dried powder 100 mg) was mixed thoroughly with 1 mL of 10% methanol contained 0.1% phosphoric acid and vortexed for 10 min. After that, the extract solution was incubated for 3 h at 37˚C with vortexing every hour. Subsequently, it was centrifuged at 12,000 rpm at 4˚C for 10 min. The supernatant was filtered through PTFE hydrophilic syringe filter (0.45 µm pore size, 13 mm diameter) prior to the quantification in HPLC.

Each peak of rapeseed meal separated using HPLC (Perkin Elmer Flexar, USA) for accurate LC-MS analysis was analyzed by 4000 Qtrap LC-MS/MS system. Mass analysis was carried out with 1.0 mL/min flow rate and positive ion mode. The UV spectrum was performed at 280 and 350 nm. The mobile phase consisted of (A) MeOH: water: acetic acid [5:92.5:2.5, (v/v/v)] + 0.01% TFA and (B) MeOH: water: acetic acid [95:2.5:2.5, (v/v/v)] + 0.01% TFA. The binary gradient used was as follows: 5% - 100% (0 - 13 min). The binary gradients were used as follows: 0 - 10 min, 0% - 20% B; 10 - 15 min, 20% - 40% B; 15 - 20 min, 40% B; 20 - 25 min, 40% - 50% B; 25 - 28 min, 50% B; 28 - 30 min, 50% - 0% B; 30 - 40 min, 0% B.

Phenolic compounds in rapeseed meal were analyzed by Perkin Elmer Flexar (USA). Samples were separated on a Capcell PAK C18 column (4.6 × 250 mm, 5 µm particle size; Shiseido, Tokyo, Japan) maintained at 40˚C. The flow rate was 1.0 mL/min, and the injection volume was 10 μL. The mobile phase consisted of (A) MeOH: water: acetic acid (5:92.5:2.5, v/v/v) and (B): water: acetic acid (95:2.5:2.5, v/v/v). Additionally, tri-floro acetic acid (TFA) was added in order to improve the peak separations. These gradients were used as follows: 0-10 min, 0% - 20% B; 10 - 15 min, 20% - 40% B; 15 - 20 min, 40% B; 20 - 25 min, 40% - 50% B; 25 - 28 min, 50% B; 28 - 30 min, 50% - 0% B; 30 - 40 min, 0% B. The absorbance was measured at 280 and 350 nm. Identification in the peaks of HPLC chromatogram of the phenolic compounds was based on spectra, standards, and literature data. External standards were used for identification and quantification, and results are presented as milligram per gram of dry weight [10] .

Acid hydrolysis procedures were done to indentify the unknown phenolic compounds. Phenolic compounds usually conjugated with sugars and organic acids were extracted by acid hydrolysis through two extraction methods (Extraction I and II) in order to eliminate the glycoside. The difference between extraction I and II was the time of adding acid into the extractions. In the Extraction I, 10% methanol contained 0.1% phosphoric acid, TBHQ (2 g/L), and 0.25 ml of 6 M hydrochloric acid was orderly added into 2.0 ml Eppendorf-tube containing rapeseed meal (100 mg), and then the mixture was extracted with vortexing for 10 min [11] . On the other hand, in the Extraction II, rapeseed meal was extracted with adding of 10% methanol contained 0.1% phosphoric acid together with TBHQ. And hydrochloric acid was then added with different ratios (1:1, 1:2, 1:3, v/v; labeled as EII-A, EII-B, EII-C, respectively) [12] . After each extraction process, the mixtures were centrifuged at 12,000 rpm at 4˚C for 15 min. Finally, the extracts were passed through a 0.45 µm PTFE ACRODISC CR13 hydrophilic syringe filter before HPLC analysis.

Phenolic compounds were identified with range of 8.69 - 19.84 retention times (RT) in total ion chromatogram, TIC (Figure 1). The RT is not matched exactly with Figure 2 and Figure 3 because HPLC analysis was per- formed with different company of analysis instrument. In addition, LC-MS was the first step in this research. The analytical method, especially entire time of the analysis, was modified reasonably after LC-MS process. The identified phenolic compounds including systematic and common names and the principal ions are listed in Table 1. Twelve phenolic and two unknown compounds were detected in all rapeseed meal extracts. Each com- pounds was identified from their protonated molecular ions [M+H]⁺ and one main productions, corresponding to the adducts [M+Na]⁺, compared to the literature information. The phenolic compounds namely [sinapine, sina- pine (4-O-8')guaiacyl, feruloyl choline(4-O-8')guaiacyl, kaempferol-3-O-sinapoyl-sophoroside-7-O-glucoside, kaempferol-3-O-β-sophoroside, unknown-1, cyclic spermidine-alkaloid, feruloyl choline(5-8')guaiacyl, kaemp-

ferol-sinapoyl-trihexoside, 1,2-di-O-sinapoylgentiobiose, 1,2-di-O-sinapoylglucose, feruloyl choline(4-O-8') guaiacyl-di-sinapoyl, unknown-2, and sinapine(4-O-8')guaiacyl-di-sinapoyl] were identified based on fragmen- tation patterns of MS spectra and quantified based on the peak areas of HPLC chromatogram (Figure 2 and Figure 3). MS data revealed that the main phenolic compounds (sinapine, kaempferol, feruloyl choline and cyc- lic spermidine-alkaloid) were conjugated with sugar such as glucose, sophorside, tri-hexoside, and guaiacyl. The HPLC peaks 1, 2, 4, 9, 10, 11, 12 and 14 have sinapoyl moiety derived from sinapine (sinapoyl choline; [M+H]⁺, m/z 310), whereas, peaks 4, 5, and 9 have kaempferol ([M+H]⁺, m/z 287). Peak 4 and 5 were identified as kaempferol-3-O-sinapoyl-sopohoroside-7-O-glucoside and kaempferol-3-O-β-sophoroside. Even though the two compounds were similar in structure, the mass spectra represented the difference in clear fragments. Their mass values (m/z) were detected at 979 in peak 4 and at 611 in peak 5 [13] , respectively. On the other hand, feruloyl choline and cyclic spermidine-alkaloid need to acid hydrolysis because of their molecular weights were not in- cluded in MS spectra library. Peaks 3, 8 and 12 were confirmed as feruloyl-choline and peak 7 has cyclic spermidine-alkaloid respectively. MS spectra of unknown-1 and 2 (peak 6 and 13) were similar to kaempferol compounds

^aNo., the elution order of flavonoid HPLC analysis in Figure 2; ^bonly detected at 280 nm, but the others detected both at 280 and 350 nm.

(especially peak 9) but kaempferol molecular weight was not detected in them. Thus the analysis using acid hydrolysis treatment was needed in order to confirm the unknown compounds.

The kaempferol and sinapine was used for quantification of phenolic compounds as external standard in rapeseed meal. The contents of phenolic compounds in rapeseed meal are shown in Table 2. Total phenolic compound content of 2014 Jeju local cultivar was the highest (63.95) and 2012 “Sunmang” was the lowest (45.78 mg/g dry weight, DW) but there is little difference of contents in each sample.

Sinapine and 1,2-di-O-sinapoyl-gentiobiose was predominant from the quantified compounds. Sinapine, one of main phenolic compounds was contained wide-ranging amounts in rapeseed meal, with levels in the following order: 2014 Jeju local cultivar (52.24) > 2014 Import (46.65) > 2011 Jeju local cultivar (41.43) > 2011 “Sunmang” (36.48) > 2012 Jeju local cultivar (34.59) > 2013 “Sunmang” (32.79) > 2012 “Sunmang” (29.74 mg/g DW). Except 2014 samples revealed highest contents, Jeju local cultivar has more amount of sinapine than “Sunmang” in each year. However total kaempferol compounds, sum of kaempferol-3-O-sinapoyl-sophoroside- 7-O-glucoside, kaempferol-3-O-β-sophoroside, and kaempferol-sinapoyl-tri-hexoside was not detected with the same tendency. Total kaempferol content was higher in “Sunmang”. On the other hand, the contents of feruloyl choline and cyclic spermidine-alkaloid were so ambiguous that there were did not reveal any tendency, whereas Oskoueian et al. (2011) reported gallic and syringic acids as phenolic acids and apigenin, kaempferol and naringenin as flavonoids in the rapeseed meal [8] .

[17] [18] reported that sinapine has anti-nutrition effects such as bitter, sour, astringency for non-ruminant animals. However [19] reported that, sinapine has beneficial effects because of the chemo-preventive, anti-tumor, anti-oxidant, and anti-mutagenic effects. Concretely, sinapine can be used as an active compound to enhance the

^aNo., the elution order of phenolics by HPLC analysis; ^bND, not detected; ^*quantified with kaempferol standard, but the others with sinapine standard. Note: In 2013, Jeju local cultivar is not cultivated and imported sample was cultivated in India.

^*Peak 1 and 2 were identified by LC-MS analysis.

^*Peak 1 and 2 were identified by LC-MS analysis.

Figure 4. The comparison of HPLC chromatogram of EII-B (sample:acid = 1:2) in 2011 Jeju mixed meal; A: before acid treatment at 280 nm, B: at350 nm, A': after acid treatment at 280 nm, B': at 350 nm.

effects of doxorubicin used for chemotherapy on cancer. Therefore, sinapine has been attracted researchers by its applications. Kaempferol is one of the flavonol belonged to flavonoids. Flavonoid compounds also have pharmacological properties such as anti-oxidative, anti-allergic, anti-inflammatory, anti-diabetic, hepato- and gastro- protective, anti-viral, and anti-neoplastic activities [11] .

Acid hydrolysis treatment was done to identify the unknown phenolic compounds present in the rapeseed meal. To confirm the unknown compounds (peak 6 and 13) acid hydrolysis treatment was conducted by various ex- traction methods. Extraction II was selected for acid hydrolysis because the peaks pattern of the extraction II was simpler and separated more clearly and the differences between extraction I and non-acid hydrolysis treat- ment samples were not significant. LC-MS analysis was performed to confirm what the peaks of HPLC chro- matogram were exactly using EII-B that presented most clear peaks among Extraction II. Sinapine (310/251/175) and feruloyl choline(4-O-8)guaiacyl (mass spectra; 476/417/221/145) were detected by LC-MS analysis. How- ever the mass spectra of other peaks were detected with different aspects from non-acid hydrolysis treatment samples, and it was hard to determine that they were certain compounds (Figure 4). That reasons were able to find from analysis data of sinapine. The content of sinapine was expected to increase after acid treatments be- cause many compounds including sinapine have sinapoyl moiety in their structure but the content were rather decreased in fact. Sinapine seems to be decomposed by high concentration of acid. Thus proper ratio of the samples to the acid will be needed for the accurate results of phenolic compound aglycone in further research.