Schistosomiasis japonica, the causative organism for schistosomiasis, is solely prevalent in Southeast Asia where an estimated two million people are infected and 69 - 75 million individuals are at risk of infection. It is unique among the major schistosomes infecting humans, as zoonotic transmission is important, with domesticated and wild animals serving as reservoir hosts of the parasite, and an amphibious snail as intermediate host. Although human infection and disease caused by S. japonicum have been reduced in China and Philippines, further reductions may be difficult because of the continual transmission from infected animals.

Normally, humans become infected with Schistosoma japonicum when cercarial forms of the parasite―re- leased by freshwater snails―penetrate the skin during contact with infested water. When active or acute infections coincide with pregnancy, the conditions also exist for the possible transplacental passage of the parasite (schistosomulae) to the developing fetus. This mode of schistosome transmission is known as vertical transmission that leads to congenital infection of the fetus and has been reported by many researchers in a wide range of animals such as mice, rabbits, guinea pigs, dogs, goats, water buffaloes and cattle [1] - [6] .

Immune relations between mothers and children have been demonstrated or detected in many diseases found in human. Many studies on different human parasitic diseases hypothesized that infection status of the mother may determine whether or not individuals who later become infected develop severe disease. A child born of a microfilaremic mother is 2.9 times more likely to become microfilaremic than a child born to an amicrofilaremic mother [7] . The same pattern is found in malaria-endemic areas where non-endemic individuals react differently from indigenous people [8] .

Studies of animal models infected with schistosome showed that their offspring have altered immunity to subsequent infection [9] . Epidemiological evidences, combined with animal experiments, indicate that the pattern of disease and pathology in human schistosomiasis relates to previous exposure and immunologic experience to the parasite. Thus, most children born in areas endemic for schistosomiasis may be born of infected mothers. Individuals who move from non-endemic to endemic areas often experience more severe acute disease and subsequent pathology than indigenous populations [10] [11] .

Intensity and duration of the infection are major determinants, but other factors are also involved. These include genetic background of the host, nutritional status, parasite strain differences, and frequency of infection. Maternal infection status has been proposed by many authors in order to explain the individual differences [12] [13] .

The aim of the present study was to assess the effect of a congenital S. japonicum infection on the resistance of rabbit kittens to a postnatal challenge infection. We assessed several immunological parameters in kittens born of Schistosoma-infected mothers in comparison to those born of non-infected ones.

Rabbits with an S. japonicum congenital infection 16 weeks previously showed a 54.66% resistant against the challenge infection. This is in keeping with the previous conclusion that the challenge infection of pigs did not result in higher worm burden and higher tissue egg counts compared to the primary control group or the challenge control group [15] . Adult worm count was significantly reduced in the offspring of Schistosoma-infected

mothers compared to mice born to non-infected ones, but no explanation was offered [16] . One possible reason for the presence or absence of severe pathology is prenatal exposure to schistosomiasis that modulates the future immune response later in life [17] . This notion has been suggested by many authors and has been explored in experimental models [18] - [20] . This could be explained by the concept of development of concomitant immunity [21] in which continuous antigenic stimulation (by the presence of some adult worms) stimulates protective immune response against further infections. Early stimulation of the immune system by in utero exposure to schistosomiasis may, therefore, provide quite strong protective immunity against initial postnatal infections. In sum, congenital exposure to Schistosoma infection seems to reduce the intensity of infection in subsequent postnatal exposure.

The congenitally-infected group also had a lower proportion of tissue eggs suggests that the previous infection status are able to destroy liver deposited eggs fairly rapidly. As considered by earlier this could be due to the host’s immunological response interfering with the uptake of essential nutrients by the parasite [22] .

The present results show that the challenge infection reduces the magnitude of granulomatous responses of a postnatal challenge infection. The same observation had been made by many researchers [16] [19] [23] - [25] . The reduced size of granulomas observed by many authors and in our experiment in congenitally exposed kittens may be attributed to attenuated Th2 response. The latter may be due to, at least in part, an increase in counter-regulatory cytokines such as IL-12 and TGF-β or could be explained by an induction of immunological tolerance [26] - [28] .

The present study shows that the congenital infection increases the IgM and IgG antibody titers compared to the exposed kittens. Modification of the immune response to schistosomal antigens, which may constitute the basis for these differences in this experiment, has been demonstrated in children born to S. mansoni-infected mothers. It has been found that Schistosoma-specific IgG levels were lower in prenatally exposed mice compared to control [16] . This in contrast to the finding that a highly significant increase in total IgG in offspring born to S. mansoni-infected mothers compared to offspring born to non-infected ones [28] . Because it was not clear what kind of antibody and how antibody is needed for protection, immunological analysis of its protection by the congenital infection would be necessary for future study.

To our knowledge, this is the first report to investigate the S. japonicum/rabbit model showing the effect of congenital infection on these variables. The findings from the present study might have wide implications for the regulatory response to infection and the associated clinical and pathological consequences of S. japonicum infection and it may assist in further exploration of mechanisms and elucidation of causative role of S. japonicum infection in the mammalian host.

These results can be relevant to humans since many children are indeed born to infected mothers in endemic areas who are rarely manifest acute manifestations such as acute dermatitis or katayama fever [29] . This will add to the complexity of the situation, and might provide a stronger background for immunomodulation in subsequent infections.

In conclusion, congenital exposure to S. japonicum infection affects the immune response and the disease outcome in the future postnatal infections; the intensity of the infection as well as the immune-pathological changes is moderated in congenitally exposed kittens. Finally, these results may give us insight for prevention and control strategies in schistosomiasis and perhaps in other chronic infectious diseases as well.