<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">OJEpi</journal-id><journal-title-group><journal-title>Open Journal of Epidemiology</journal-title></journal-title-group><issn pub-type="epub">2165-7459</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/ojepi.2013.34030</article-id><article-id pub-id-type="publisher-id">OJEpi-39858</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine&amp;Healthcare</subject></subj-group></article-categories><title-group><article-title>
 
 
  Assessment of serum calcium in relation to dietary calcium intake in a rural population in Cameroon
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>iba</surname><given-names>Rawlings Ngwa</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Akwa</surname><given-names>Emmanuela Ambe</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib></contrib-group><aff id="aff1"><addr-line>HAMANI Foundation Training School of Health, Santa, Cameroon</addr-line></aff><author-notes><corresp id="cor1">* E-mail:<email>nbngwa@gmail.com(IRN)</email>;</corresp></author-notes><pub-date pub-type="epub"><day>15</day><month>11</month><year>2013</year></pub-date><volume>03</volume><issue>04</issue><fpage>209</fpage><lpage>212</lpage><history><date date-type="received"><day>19</day>	<month>September</month>	<year>2013</year></date><date date-type="rev-recd"><day>19</day>	<month>October</month>	<year>2013</year>	</date><date date-type="accepted"><day>26</day>	<month>October</month>	<year>2013</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
  The serum calcium levels of patients were assessed as they attended a district hospital in a rural population for a period of one month. A majority of the patients were between the age group of 38-77 years. 55 cases were studied. The ratio of males to females was 3:8. The results show an increase in abnormal serum calcium level with age. The abnormality in serum calcium was higher in females than in males. The demonstration of poor nutritional intake and lack of other calcium supplements was a predisposing factor to the abnormal calcium levels. It was concluded that the abnormal levels of calcium were as a result of lack of calcium diet.
 
</p></abstract><kwd-group><kwd>Calcium; Dietary Intake; Ratio; Electrolyte; Osteoporosis</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. INTRODUCTION</title><p>Calcium is one of the main electrolytes in body fluids. In the body, 99% of calcium is found in bones for the mineralization of bones [<xref ref-type="bibr" rid="scirp.39858-ref1">1</xref>]. The remaining 1% is found in body fluids where it is important for neuromuscular excitability, cardiac activity, blood coagulation and membrane permeability [2,3]. Calcium circulates in plasma in two forms: calcium bound to albumin and free ionized calcium. Serum calcium ranges from 8.8 to 10.4 mg/dl (2.2 to 2.6 mM) in healthy subjects. It comprises free ions (51%), protein-bound complexes (40%), and ionic complexes (9%) [<xref ref-type="bibr" rid="scirp.39858-ref4">4</xref>]. Ionized calcium has a normal value of 4.5 - 5.5 mg/dl (1.1 - 1.4 mmol/L). This value is usually affected by a number of factors including parathyroid hormone and calcitonin secretion, dietary intake and conditions like osteoporosis. Bone mineral content increases throughout childhood [<xref ref-type="bibr" rid="scirp.39858-ref5">5</xref>], peaks in adolescence [<xref ref-type="bibr" rid="scirp.39858-ref6">6</xref>], remains relatively constant in early/late adulthood</p><p>[<xref ref-type="bibr" rid="scirp.39858-ref7">7</xref>], and declines in old age. Dietary intake and absorption are essential to provide sufficient calcium to maintain healthy body stores. Approximately 30% of dietary calcium ingested in a healthy adult is absorbed by the small intestine. Calcium absorption is a function of active transport that is controlled by 1,25(OH)2D, which is particularly important at low calcium intakes, and passive diffusion, which dominates at high calcium intakes. Typically, at normal calcium intake, 1,25(OH)2D-dependent transport accounts for the majority of absorption, whereas as little as 8% to 23% of overall calcium absorption is caused by passive diffusion [<xref ref-type="bibr" rid="scirp.39858-ref8">8</xref>]. Because almost all dietary calcium intake is absorbed from the upper intestine, frequent meals or oral supplements promote net calcium absorption. The bioavailability of dietary calcium can be enhanced. Aluminum hydroxide, which binds dietary phosphate [<xref ref-type="bibr" rid="scirp.39858-ref9">9</xref>], when taken in excess leads to hypercalciuria from increased calcium absorption [<xref ref-type="bibr" rid="scirp.39858-ref10">10</xref>].</p><p>The people of the rural population under study live on yams as their main source of food. The population of this area is made up of elderly people due to rural-urban migration of the young and middle age adults in search for greener pastures. These people frequent the hospital due to pains in their muscles and bones.</p></sec><sec id="s2"><title>2. MATERIALS AND METHODS</title><sec id="s2_1"><title>2.1. Sample Size</title><p>A study of 55 cases with age range from 38 - 77 years and belonging to both genders was conducted on “serum calcium in relation to their diet” at a district hospital. The study was carried out from April to May 2013.</p></sec><sec id="s2_2"><title>2.2. Data Collection</title><p>Patients who were registered to the laboratory for serum calcium test responded to questionnaires from the researcher. The questionnaires were filled face to face with the respondents by the researcher and one assistant. It included information on usual diet intake and physical wellbeing of the respondents.</p><p>For the question on usual diet, the responses were categorized into 1) yams only 2) yams with meat or fish 3) yams, meat or fish and other milk products.</p><p>For questions on the physical wellbeing, responses were categorized into 1) physically fine 2) mild body pains 3) serious body pains.</p></sec><sec id="s2_3"><title>2.3. Statistical Analysis</title><p>Statistical analysis of data generated was done with statistical package for social sciences (SPSS). The data was analyzed using Analysis of Variance (ANOVA). P &lt; 0.05 is considered significant.</p></sec><sec id="s2_4"><title>2.4. Estimation of Serum Calcium</title><p>Venous blood was collected in plain vacutainers and serum was immediately separated by centrifugation. The serum samples were then stored at 4˚C and analyzed within 24 hours.</p></sec></sec><sec id="s3"><title>3. RESULTS</title><p>Over a period of one month, 55 cases were analyzed for serum calcium levels. The age group of the cases ranged from 38 - 77 years with a males-to-females ratio of 3:8. There was an increase in abnormality of calcium levels with age shown on <xref ref-type="table" rid="table1">Table 1</xref>. From the ratio of males to females as shown on <xref ref-type="table" rid="table2">Table 2</xref>, serum calcium abnormality is higher in females than in males. Osteoporosis was the most common comorbid condition followed by muscle spasm and tetany as seen on <xref ref-type="table" rid="table3">Table 3</xref>. From the responses shown on <xref ref-type="table" rid="table4">Table 4</xref> the people of this area live sorely on yam produce and rarely on meat, fish and other milk products. <xref ref-type="table" rid="table5">Table 5</xref> shows the recommended calcium level for various age groups. From the responses of the people in this area as seen on <xref ref-type="table" rid="table4">Table 4</xref>, there is no doubt that their calcium is not within the recommended levels since they don’t get dietary supplements.</p></sec><sec id="s4"><title>4. DISCUSSION</title><p><xref ref-type="fig" rid="fig1">Figure 1</xref> shows that abnormality in serum calcium increases with age. This confirms the fact that elderly peo-</p></sec></body><back><ref-list><title>References</title><ref id="scirp.39858-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">Wang, L., Nancollas, G.H. and Henneman, Z.J. (2006) Nanosized particles in bone and dissolution insensitivity of bone mineral. Biointerphases, 1, 106-111. 
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