When life first originated on the Earth, the first forms of life appeared in water and thus did not need to adapt to dehydration, gravity, UV or NIR as the surrounding water served as a barrier against these obstacles. The need to overcome these obstacles arose for the first time when primitive life developed on land. Every species of terrestrial life has acquired fibrous structural proteins rich in hydrogen bonds and alpha helices: cellulose for plants, fibrous chitin and proteins for insects, and keratin for reptiles, birds, amphibians, and mammals. These components are biologically-economical, practical and beneficial to overcome the obstacles.

The fibrous structural proteins rich in hydrogen bonds and alpha helices are particularly convenient for NIR blocking because hydrogen bonds and alpha helices efficiently absorb NIR. For instance, sheep prefer the sun to the shade, and even though the animals remain in sunny environments without much perspiration, their body temperature does not become elevated, as sheep wool consists of alpha helical structures that efficiently block and absorb NIR.

Humans have acquired protective mechanisms against NIR on multiple levels, including perspiration, vasodilation, hair, skin, adipose tissue, and wearing cotton or wool, which are all rich in hydrogen bonds and alpha helices [Figure 1].

NIR induces perspiration to increase water retention superficially and decrease superficial temperature, and subsequently induces vasodilation to increase water and hemoglobin retention as a NIR absorbent. In the dermis,

NIR induces accumulation of collagen, elastin and waterbinding proteins, which possess helical structures rich in hydrogen bonds [1,2,4]. Over the long-term, NIR induces subcutaneous adipocytes located above superficial muscles, which are effective for NIR blocking, because adipose tissue can scatter NIR optically [11].

The effects of NIR are independent of generated heat [12]. NIR non-thermally induces degeneration of myoglobin, resulting in apoptosis of vascular smooth muscle cells and long-lasting vasodilation [Tanaka]. Our collagen, elastin, and cancer studies suggest that NIR may mainly resonate hydrogen bonds, helical structures, alpha helices, and DNA [1,2,4]. Alpha helices are thought to be resonated by NIR [34]. Both hemoglobin and myoglobin are oxygen-carrying proteins with many hydrogen bonds and alpha helices. It is possible that NIR induces resonance of helical structures in the oxygen-carrying proteins and degenerates proteins containing hydrogen bonds and helical structures, which results in damage to the storage and transport of oxygen. This could be one of the mechanisms of apoptosis [1,2].

In addition to the skin, the optic nerve, which is the only exposed component of the central nervous system, also has protective mechanisms against NIR on multiple levels. These mechanisms include rich blood flow in the retina, hyaluronic acid in the lens, tears in sclera, and adipose tissue in the eyelid. These are effective protective mechanisms, as water, hemoglobin, and fatty acid are all ideal materials rich in hydrogen bonds and alpha helices for blocking NIR exposure [2].

NIR exhibits both wave and particle properties and appears to resonate hydrogen bonds and alpha helices [Figure 1], and therefore can affect the deeper tissues and induce various biological effects. The NIR spectrum is a result of the overtones and combination of bond stretching vibrations from O-H, C-H, and N-H groups [35].

Humans have biological protective mechanisms against NIR that absorb NIR by induction of substances rich in hydrogen bonds and alpha helices, or reflect NIR by induction of fatty acid to protect the subcutaneous tissues against NIR [1,2]. NIR is absorbed by water in perspiration and blisters, hemoglobin and water in vasodilation, keratin in hair and the horny layer of the skin, and water-binding proteins and water in the dermis, and is reflected by adipose tissue [Figure 1].

The cell nucleus possesses a nuclear lamina underneath the inner nuclear membrane. The nuclear lamina contains lamins, which have a conserved alpha helical central rod domain and variable head and tail domains [36-38], that form a stress-resistant elastic network [36]. Lamins play important roles in DNA replication, chromatin organization, adult stem cell differentiation, aging, and tumorigenesis. In addition, mutations in lamin lead to laminopathic diseases [36]. Nuclei assembled in the absence of lamins are more prone to breakage than nuclei assembled in the presence of a full complement of lamins [39,40]. Disruption of lamins results in abnormal mitosis, chromosomal segregation, and cell death [41]. Thus, lamins absorb NIR and protect DNA in the nucleus from NIR [1] [Figure 2].

During mitosis, lamin molecules are transiently disassembled into monomers [42,43]. NIR induces non-thermal DNA damage of mitotic cells in prophase, metaphase, and anaphase due to the absence of nuclear lamin protection against NIR, which results in apoptotic cell death [1]. This could be one of the mechanisms of apoptosis in cancer [1].

Further studies are required to fully investigate the biological responses to NIR in humans.

NIR induces dermal heating and induces collagen and elastin stimulation, resulting in skin tightening [10,19, 44,45]. NIR induces high collagen density in the dermis, leading to epidermal smoothness without scar formation, which provides safe, consistent, and long-term effects of skin rejuvenation [20,21]. Pre-exposure of NIR prevents UV-induced toxicity [30,46,47], and this effect is independent of heat shock protein induction and cell division [47]. These findings support the hypothesis that NIR prepares skin to better resist the subsequent damage from UV or NIR [1].

NIR can treat musculoskeletal disorders and healing of indolent wounds [48,49]. NIR induces long-lasting vasodilation for an increase in blood circulation by causing apoptosis of vascular smooth muscle cells, which may prevent vasospasm and may be beneficial for ischemic disorders [2]. NIR activates mitochondrial metabolism [50-53], assists wound healing, and promotes angiogenesis in skin [54], bone [55], nerve [56], and skeletal muscle [57,58]. NIR also relaxes and weakens dystonic or hypertrophic muscles to reduce wrinkles and myalgia.

Thus, the simple technique of using NIR may offer an alternative method to relax superficial muscles [7].

We previously reported that NIR increased subcutaneous adipocytes on the superficial muscles as well as CD34-positive stem cells surrounding the subcutaneous adipocytes, which may have an application for volume augmentation [11]. Further, NIR increased subcutaneous and bone marrow adipocytes and CD34-positive hematopoietic stem cells in bone marrow, and cortical bone mass [11]. Stimulation of stem cells by NIR might be beneficial for applications in regenerative medicine [2].

NIR is also an essential tool in cancer detection and imaging, as actively proliferating cells show increased sensitivity to NIR [59,60].

The most common anti-tumor therapy using NIR is photodynamic therapy (PDT) based on the accumulation of a photosensitizer in tumors, which is effective for some forms of cancer [61]. PDT uses wavelengths near 800 nm as a photoactivating wavelength to achieve maximum penetration depth [62], but this wavelength also has high melanin absorption, limiting the ability to deliver light to highly pigmented tumors [63]. Shorter wavelengths are the standard activators for PDT but other wavelengths have shown treatment promises. NIR at a wavelength of 904 nm has anti-tumor activity and increases cytomorphological changes by inducing apoptosis in neoplastic cells [64]. In addition, actively proliferating cells show increased sensitivity to NIR [59,60]. NIR induces DNA strand breaks and cell death by apoptosis [65], eliciting photodisruptive destruction of tumor tissue [66]. Unlike wavelengths beyond 1100 nm where melanin absorption is negligible [8], absorption at 904 nm is significant. This may limit possible uses of the 904 nm wavelength for certain body areas in races with skin rich in melanin [4,22,23].

NIR from 1100 to 1800 nm together with a water-filter that excludes wavelengths between 1400 and 1500 nm induces non-thermal DNA damage of mitotic cells particularly in prophase, metaphase, and anaphase due to the absence of nuclear lamin protection [1], and suppresses the proliferation of various kinds of malignant cells [22, 23]. While NIR damages tumor tissue, it is also shown to reduce cellular protein damage produced by biological oxidants in normal cells [67].

NIR may be beneficial in multiple fields of medicine, as the schedule reduces discomfort and side effects, deep subcutaneous tissues are accessible, and the approach enables repeated irradiations [2].

As NIR has a very wide range of wavelengths, the wavelength or fluence should be investigated, in order to induce beneficial effects without deleterious effects. Further, conditions and parameters should be also evaluated, as higher output, increased frequency of treatments, and/ or longer periods of irradiation may induce deleterious effects.

These findings indicate that NIR has a wide range of biological effects, and future studies are warranted to develop these findings into beneficial techniques and applications.

NIR performs as an aging factor, where biological NIR protection is not complete. NIR induces photoaging similar to that observed in solar elastosis, and enhances UVinduced dermal damage [32]. NIR activates mitogen-activated protein kinases and induces gene transcription, and appears to increase collagen degradation [24,25,68].

Long-term exposure to sources of heat and NIR, such as fires and stoves, results in erythema abigne [69], which is characterized by a reticular hyperpigmentation and telangiectasia accompanied by epidermal atrophy, vasodilation, dermal melanin and hemosiderin deposits [1]. Longterm exposure of NIR from various heat sources is thought to induce reticulated erythema and result in histopathological changes similar to those seen in solar-damaged skin [70]. The occurrence of telangiectasia appeared to increase with age, increased sunbathing, and poor pigmentation ability [71].

Similar to UV, NIR induces photoaging and potentially photocarcinogenesis [24]. In addition, skin tumors appeared faster after irradiation with the full lamp spectrum containing UV, visible, and NIR compared to irradiation with UV alone [72]. NIR induces non-thermal DNA damage and drastic cell death of mitotic cancer cells, and cancer stem cells might be activated to maintain homeostasis. 

NIR is attenuated by thick water-containing dermis. Thus, skin with a thin dermis allows NIR to penetrate deeper into tissue than skin with a thick dermis [1]. The mean area of the facial surface covered with wrinkles is significantly larger in Caucasians than in African Americans, and characteristics of age-related periorbital changes seem to occur at a more accelerated rate in Caucasians [73]. In addition, fair skin is more sensitive to skin aging [74,75]. These findings support the observation that fair skin tends to wrinkle and sag earlier in life [76,77], as fair skin is thinner and more susceptible to NIR damage to the underlying superficial muscles than dark skin [1].

Continual long-term exposure to solar NIR causes superficial muscle thinning, which ultimately leads to skin ptosis [6]. Although further studies are required to confirm our results, these results may have major implications in superficial tissue aging and skin ptosis.

NIR produces clouding of the cornea and cataract, as NIR is easily absorbed by the anterior segment, such as the cornea, aqueous, and lens [78-80].

As over half of the solar energy is NIR and biological effects of NIR are significant, NIR protection should be performed on multiple levels, including perspiration, vasodilation, hair, skin, adipose tissue, sunblock, sunglasses, films, and clothing.

Hints of effective NIR blocking are hidden in the evolutionary history of life. Every species of terrestrial life has acquired fibrous structural proteins, such as cellulose, chitin, and keratin during evolution. These substances rich in helical structures and hydrogen bonds are biologically-economical, practical and, beneficial to block NIR.

Humans have been naturally protected from NIR by perspiration, keratin, hemoglobin, water-binding proteins, such as collagen, and elastin, and by using clothing made from biological NIR blocking materials, such as cellulose and keratin. Removing hair and the horny layer of the skin decreases protection abilities against NIR.

Other than the biological materials for NIR block, metal oxides such as titanium oxide, zinc oxide, compounds containing silicon, and organic compounds are common in the industrial field. They can block both thermal and non-thermal effects of NIR. NIR is also emitted from monitors, screens, and electrical appliances, so materials for NIR block often require transparency.

Demand for transparent materials for NIR blocking is and will be increasing as the biological effects of NIR become widely known. However, supply of these materials may not be sufficient. A common transparent material for blocking NIR is compounds containing indium, though its supply is limited due to various factors, such as scarcity and environmental constraint. Alternatives to indium should be developed and are urgently required.

A variety of light and heat blocking materials, such as sunblock, sunglasses, films, and fibers, should equip protection ability against not only UV but also NIR, and should be widely prevalent for prevention against aging and carcinogenesis.