AJMBAmerican Journal of Molecular Biology2161-6620Scientific Research Publishing10.4236/ajmb.2012.23025AJMB-20969ArticlesBiomedical&Life Sciences The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in <i>Arabidopsis thaliana</i> aríaI. Vaquero-Sedas1MiguelA. Vega-Palas1*Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Seville, Spain* E-mail:vega-palas@ibvf.csic.es(MAV);27072012020324224419 April 20123 May 2012 24 May 2012© Copyright 2014 by authors and Scientific Research Publishing Inc. 2014This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/

We have previously described a simple method to analyze the chromatin structure of Arabidopsis telomeres independently of that of Interstitial Telomeric Sequences (ITSs). By using this method, we found that, whereas ITSs are heterochromatic, Arabidopsis telomeres exhibit euchromatic features [1]. Some concerns have been recently raised about the accuracy of this procedure [2]. Here, we summarize these concerns and justify our experimental approaches and interpretation of results.

<i>Arabidopsis thaliana</i>; telomeres; ITSs; euchromatin; heterochromatinACKNOWLEDGEMENTS

This research was supported by the Spanish Ministry of Science and Innovation grant BIO2011-24794 and by FEDER funds.

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