This was a single-arm, prospective, interventional study conducted from July 1, 2024, to July 31, 2025 (13 months) at the CNRSD in Togo, located in Lomé. This facility served as the site for recruitment, anthropometric measurements, bioimpedance analysis, and follow-up of malnourished patients with sickle cell disease who came to pick up their flour kits. The Togolese Institute of Agricultural Research (ITRA) produced the batches of flour needed for ongoing distribution. The National Institute of Hygiene and Public Health (INH) served as the site for conducting biochemical tests.

The weight of children and adults with sickle cell disease was measured using an M321600 electronic scale (ADE Germany, China) with a digital display capable of weighing up to 250 kg in 0.1-kg increments. Height was measured using a Shorr height rod with 0.1-cm increments. Arm circumference was measured using a Shakir tape-. Harpenden calipers were used for triceps skinfold thickness, and the Z-Metrix (Bioparhom, France) was used for body composition analysis. The Cobas c311 and e411 biochemical and immunochemical analyzers (Roche Diagnostics, Switzerland) were used for the various biochemical assays. The Minicap Flex Piercing (SEBIA, France) was used to perform protein electrophoresis. Data were collected during face-to-face interviews using a pre-established questionnaire. The questionnaire was pre-tested, including reproducibility exercises for the use of the Harpenden caliper. At the end of each day, the completed forms were reviewed and corrected as necessary to ensure the completeness and accuracy of the data.

2.3.1. Study Population

Based on the 10% attendance rate of individuals with sickle cell disease seen at the CNRSD representing 343 subjects expected for the assessment of protein-energy nutritional status a study population size consistent with the prevalence of sickle cell disease was determined. Taking into account the estimated prevalence of major sickle cell disease of between 3% and 5% [16] and using Cochran’s formula, a sample size of 73 patients was determined.

2.3.2. Inclusion Criteria

The study population consisted of HbSS and HbSC sickle cell patients. The inclusion criteria were: a confirmed diagnosis of sickle cell disease by hemoglobin electrophoresis; the presence of malnutrition diagnosed according to at least one validated WHO criterion, primarily through anthropometric measurements (weight, height, BMI, upper arm circumference, and triceps skinfold thickness) and secondarily through body composition assessment and measurement of certain biochemical parameters. Age over 5 years, and informed consent from the patient or their legal guardian.

2.3.3. Exclusion Criteria

Patients with severe uncontrolled comorbidities, those on immunosuppressive therapy, or those who had received a transfusion within the 3 months prior to enrollment were excluded.

2.3.4. Follow-Up Method

Selected patients with sickle cell disease underwent assessment of sociodemographic information, dietary habits, and anthropometric measurements (weight, height, BMI, upper arm circumference, and triceps skinfold thickness) to diagnose malnutrition. Once consent was obtained for a 6-month follow-up, a blood sample was collected and body composition was measured using bioelectrical impedance analysis for each subject at the beginning and end of the 6-month follow-up period. Kits containing 100 g of enriched flour with anti-inflammatory and antioxidant properties, produced by [15], were provided at a rate of 2 kits per day for adolescents and children under 18 years of age and 3 kits per day for adults over 19 years of age during the 6-month follow-up period. Biochemical testing methods were used to assess malnutrition and inflammatory processes.

2.3.5. Vaso-Occlusive Crises (VOCs)

Vaso-occlusive crises (VOC) were defined as any painful episode of sufficient intensity to require analgesic treatment and/or a medical consultation or hospitalization, lasting at least two hours, with no other identifiable cause [17]. The baseline VOC rate (prior to intervention) was determined through review of the medical records of participants followed at the CNRSD, as well as through structured interviews conducted at enrollment, during which patients reported the number of VOC episodes occurring in the six months preceding their inclusion. Following enrollment, participants were monitored over a six-month follow-up period through scheduled visits (T1 to T5) for flour stock renewal and verification of any use of transfusion or hydroxyurea, supplemented by regular telephone contacts between visits and a follow-up booklet provided to each patient. Any VOC episode occurring during the follow-up period, including those managed outside the study center, was systematically recorded through these various means.

2.4.1. Anthropometric Measurements

Measurements were performed according to WHO procedures [18]. Malnutrition was assessed using a three-tier classification (severe, moderate, normal) based on five indicators: weight-for-length z-score (12 - 59 months), height-for-age z-score (5 - 18 years), body mass index (BMI) (≥ 19 years), upper arm circumference (UAC), and triceps skinfold thickness (TSFT). The TSC thresholds were based on WHO growth charts for children under 5 years of age, the tables by Soylu et al. (2021) for 6 - 18-year-olds [19], and the reference values from Hasselmann & Alix (2003) for adults aged 19 and older, defining depletion of fat reserves as a value below 50% of the mean reference value for the respective sex (i.e., <6 mm in men and <11.5 mm in women) [20][21]. A patient was classified as malnourished if they exhibited severe or moderate malnutrition on at least one indicator.

2.4.2. Body Composition Measurements: Bioimpedance Analysis

Impedance measurement is a method of measuring resistance to the flow of an electric current. This test relies on the electrical properties of biological tissue and involves passing a harmless, low-intensity electric current (approximately 70 mA) through the body via electrodes in contact with the skin, and measuring its conduction within the body. The impedance measurement was performed using electrodes placed precisely on the limbs, with the subject standing with legs slightly apart. Electrode placement: four electrodes labeled 1, 2, 5, and 6 are placed on the skin. Electrodes 1 and 2 (higher up) are placed on the hand, and electrodes 5 and 6 (lower down) are placed on the ankle. Formulas are then used to convert the impedance, which measures body water into an estimate of the fat mass. The Metabolic Activity Index (MAI) indicates healthy ion channels, the ability to restore osmotic pressure, and proper transfer of ions and proteins. MAI < 4.63 (abnormal metabolic activity) MAI 4.63 - 7.63 (reference range).

Muscle Mass (MM) or Muscle Mass Index (MMI) measures the total weight of the cells that make up the muscles and is a good indicator of lean body mass. Muscle Mass Index (MMI):

M < 7.0 and F < 5.7

Non-fat mass represents lean body mass (LBM), which accounts for visceral protein mass, intracellular and extracellular water, and bone mass. It includes muscles, bones, organs, water, and blood, and is used to assess malnutrition in obese individuals. Non-fat mass index: M < 17, F < 15 kg/m².

Fat Mass (FM) or Fat Mass Index (FMI) is the amount of fat in the body; it is a better indicator of body composition than BMI, since BMI does not distinguish between muscle mass and fat mass. A decrease in FM is the best indicator of weight loss.

2.4.3. Evaluation Using Biochemical Measures

The following tests were performed on the blood samples collected, taking into account the standard reference values [22][23].

Spectrophotometry: albumin, calcium, magnesium, phosphorus,Particle-based immuno-turbidimetry: CRP and ferritin;Indirect potentiometric ISE: sodium, potassium, and chloride;Capillary technique using Minicap Flex Piercing: protein electrophoresis. The relevant fractions analyzed were Alpha 1 and Alpha 2 for monitoring the inflammatory status.

1)Albumin

Principle: At a pH of 4.1, albumin is sufficiently cationic to combine with bromocresol green (BCG) in the form of an anion to form a blue-green complex [24]. The intensity of the blue-green color developed is directly proportional to the albumin concentration and is measured by photometry. Wavelengths (sec/primary) 505/570 nm.

Reference range: Adults 35 - 52 g/L (532 - 790 μmol/L).

Interpretation: Moderate malnutrition 30 - 35 g/L, Severe malnutrition 25 - 30 g/L.

2)Calcium

Principle: Calcium ions react with 5-nitro-5'-methyl-BAPTA (NMBAPTA) in an alkaline medium to form a complex. Subsequently, this complex reacts in the presence of EDTA. The intensity of the color developed by the complex is directly proportional to the calcium concentration and is measured by photometry. Wavelengths (absorbance/reference) 376/340 nm.

Reference range depend on age.

0 - 10 days 76 - 104 mg/L

10 days - 2 years 90 - 110 mg/L

2 - 12 years 88 - 108 mg/L

12 - 18 years 84 - 102 mg/L

18 - 60 years 86 - 100 mg/L

60 - 90 years 88 - 102 mg/L

>90 years 82 - 96 mg/L

Interpretation: Hypocalcemia below reference values

Hypercalcemia above reference values

3)Magnesium

Principle: Magnesium ions form a purple complex in an alkaline medium with xylidyl blue (diazonium salt). Magnesium concentration is measured photometrically by the decrease in the absorbance of xylidyl blue [25]. Wavelength (sec/princ) 505/600 nm.

Reference range depend on age.

5 months - 6 years 15 - 22 mg/L

6 - 12 years 17 - 23 mg/L

12 - 20 years 17 - 21 mg/L

20 - 60 years 17 - 22 mg/L

60 - 90 years 1 - 24 mg/L

>90 years 17 - 23 mg/L

Interpretation: Hypomagnesemia below reference values

Hypermagnesemia above reference values

4)Phosphorus

Principle: In the presence of sulfuric acid, inorganic phosphate reacts with ammonium molybdate to form an ammonium phosphomolybdate complex with the formula (NH₄)₃[PO₄(MoO₃)₁₂]. The concentration of phosphomolybdate formed is directly proportional to the concentration of inorganic phosphate and is measured by UV photometry [26]. Wavelength (sec/princ) 700/340 nm.

Reference range depend on age.

Infants: 50 - 70 mg/L

Children: 32 - 57 mg/L

Adults: 25 - 45 mg/L

Interpretation: Hypophosphatemia below reference values

Hyperphosphatemia above reference values

5)C-Reactive Protein (CRP)

Principle: Immunoturbidimetric assay using on latex particles. Human CRP binds on latex particles coated with anti-CRP monoclonal antibodies. The agglomerates of particle are measured by turbidimetry [27][28]. Wavelength (sec/princ): 800/570 nm.

Reference range for adults: <6 mg/L (<57.12 nmol/L)

Interpretation: Acute inflammation, CRP > 6 mg/L

6)Ferritin

Principle: Immunoturbidimetric assay using latex particles. Human ferritin binds to latex particles coated with anti-ferritin antibodies. The precipitate is measured by turbidimetry at 570/800 nm [29].

Reference rang

Men (20 to 60 years): 30 - 400 μg/L (67 - 899 pmol/L, 30 - 400 ng/mL)

Women (17 to 60 years): 15 - 150 μg/L (34 - 337 pmol/L, 15 - 150 ng/mL)

Interpretation: Hypoferritinemia below reference values

Hyperferritinemia above reference values

7)Sodium Na⁺, Potassium K⁺, Chloride Cl⁻

Principle: Ion-selective electrode, using automatically diluted serum/plasma or urine samples. The sodium, potassium, and chloride electrodes are based on ion-exchange systems [30]-[33].

Calculation: The equation below is used for calculating sample and/or quality control (QC) results:

where:

CS: Concentration of the ion in the sample

CIS: Concentration of the ion in the ISE (Internal Standard Electrode) solution

ES: Electromotive Force (EMF) of the sample

EIS: EMF of the Internal Standard Electrode (ISE) solution

S: Slope of electrode

Log: decimal logarithm

The complete measurement system for a specific ion consists of the ISE, a reference electrode, and electronic circuits that measure and process the EMF to determine the concentration of the measured ion (K⁺, Cl⁻, Na⁺).

Reference rang

Sodium

Normal 136 - 145 mmol/L

Interpretation: Hyponatremia below reference values

Hypernatremia above reference values

Potassium

Normal 136 - 145 mmol/L

Interpretation: Hypokalemia below reference values

Hyperkalemia above reference values

Chloride

Adult 98 - 107 mmol/L

>90 years 98 - 111 mmol/L

Interpretation: Hypochloremia below reference values

Hyperchloremia above reference values

2.4.4. Protein Electrophoresis

The basic principles of separation were:

Electric field: Proteins migrate through a capillary filled with electrolyte under the influence of a high voltage.Electrophoretic mobility: The migration rate depends on the charge and size of each protein.Electroosmotic Flow (EOF): EOF, a fluid flow caused by charges on the capillary wall, accelerates or decelerates the molecules, enabling the detection of all fractions.Detection: A UV detector at the capillary outlet measures the absorbance of the separated proteins, generating an electropherogram.

The presence of an inflammatory state by protein electrophoresis was assessed as follows:

Moderate inflammatory syndrome: alpha-1 > 4 g/L and < 6 g/L and/or alpha-2 > 9 g/L and < 12 g/LSevere inflammatory syndrome: alpha-1 > 6 g/L and/or alpha-2 > 12 g/LInflammatory state due to C3 hypercomplementemia: 5 g/L < Beta2 < 8 g/LPrevalence of hypoalbuminemia: Moderate (albumin < 35 g/L and >30 g/L), Severe (albumin < 30 g/L),hypergammaglobulinemia: moderate (gamma > 15 g/L < 20 g/L), severe (>20 g/L).

The enriched flour was formulated using six local ingredients, selected for their complementary nutritional, anti-inflammatory, and antioxidant properties, which have been documented in the scientific literature. The detailed composition is presented in Table 1.

Table 1. Composition of 100 g of enriched flour and profile of active compounds [15].

The data were entered and analyzed using appropriate statistical software. Quantitative variables are expressed as mean ± standard deviation (x̄ ± SD). Before-and-after comparisons were performed using the Wilcoxon test for quantitative variables and the McNemar test for paired categorical variables. Mated odds ratios (OR) were calculated as the ratio of discordant pairs (b/c), with 95% confidence intervals (95% CI) obtained using the Wald method on a logarithmic scale. The rate of change (RC) was defined as ( y ¯ − x ¯ ) / x ¯ . The threshold for statistical significance was set at p < 0.05.

A total of 233 patients with sickle cell disease participated in the study, including 119 (51.07%) males and 114 (48.93%) females, for a sex ratio of 1.04 males to 1 female.

Among the 233 participants, the prevalence of malnutrition based on at least one diagnostic criterion was 66.09% (n = 154). Of the 154 malnourished patients, 75 (48.7%) agreed to undergo laboratory testing and body composition analysis using bioimpedance analysis. Of these, 57 (76%) attended the 6-month follow-up appointment, while 18 participants were lost to follow-up. Figure 1 shows the flowchart of participants.

The distribution of the 57 and 75 patients by age group and genotype is presented in Table 2.

Figure 1.Participant flowchart.

Table 2. Distribution of patients by age group, genotype, and sex (75 = enrollment; 57 = 6-month follow-up).

Initial biochemical evaluation of the 75 undernourished patients revealed significant abnormalities:

Electrolyte imbalances: hyperkalemia in 28%, hyponatremia in 32%, hypochloremia in 29%, hypocalcemia in 9.33%, and hypercalcemia in 8%.Acute inflammatory syndrome: 46.67% of patients had a CRP > 6 mg/L, 42.66% had elevated alpha-1 and/or alpha-2 glycoproteins, and 68% had hypercomplementemia.Chronic inflammatory syndrome: 72% had hypergammaglobulinemia.Bioelectrical impedance analysis: At least one tissue, fluid, or metabolic index was abnormal in 96% of undernourished patients.

After 6 months of consuming the enriched flour, a significant improvement was observed in all inflammatory markers among the 57 subjects who returned for the final follow-up. Table 3 presents the results, showing the decrease in the proportion of subjects exhibiting an inflammatory (PEI) state after consuming the flour.

A total of 13,180 kits were used out of the 25,200 planned for the initial cohort of 75 patients over a 6-month period, representing an overall utilization rate of 52.30%. Table 4 presents the comparative results before and after the intervention

Table 3. Impact of consuming enriched flour on inflammatory markers.

PEI: pathological inflammatory element. a, b, c, d: cells in the 2 × 2 paired contingency table (before × after). The paired OR is calculated as b/c (discordant pairs), with a 95% CI obtained using the Wald method on a logarithmic scale. *Exact McNemar’s test. ^†McNemar’s χ² test with continuity correction. a: PEI → PEI, b: PEI → Normal, c: Normal → PEI, d: Normal → Normal

Table 4. Impact of enriched flour on biochemical and anthropometric parameters.

X ¯ : average of the values before adding the flour; Y ¯ : mean of values after consuming the flour.

for the main biochemical and anthropometric parameters, as well as the frequency of vaso-occlusive crises (n = 57).

The bioelectrical impedance analysis at 6 months revealed significant improvements in body composition (Table 5).

Table 5. Impact of enriched flour on body composition parameters (N = 57).

x: number of patients in a normal state before consuming the flour kits. y: number of patients in normal condition after taking the flour kits.

The prevalence of malnutrition observed in our cohort (66.09%) is consistent with data from the African literature. A systematic review conducted according to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) criteria, covering the period 1995-2020, reported that African studies consistently described malnutrition and stunted growth among pediatric and adolescent patients with sickle cell disease, though no robust interventional studies were identified [9]. In Nigeria, an analysis of data from the Demographic and Health Survey which included neonatal screening for sickle cell disease for the first time showed that the prevalence of stunting and underweight was 55.4% and 38.9%, respectively, among children with sickle cell disease, with a 2.39-fold higher risk of stunting (adjusted OR: 2.39; 95% CI: 1.26 - 4.54) than among children without sickle cell disease [10]. The prevalence found in the present study exceeds these figures, which can be explained by the inclusion of adults in the cohort and the use of sensitive diagnostic criteria (TSFT, PB, BMI-for-age, weight-for-height z-score) in accordance with WHO recommendations [9].

The predominance of TSFT as the most sensitive criterion (58.80%) was notable. This result suggests that malnutrition in our population is primarily of the marastic type, characterized by loss of subcutaneous adipose tissue, which can be explained by chronic hypermetabolism associated with hemolysis and repeated episodes of infection. The imbalance between nutritional intake and requirements in sickle cell disease is well documented, resulting from a combination of increased energy expenditure (hemolysis: 20% - 40% of basal metabolism), reduced food intake during crises, and malabsorption associated with episodes of intestinal ischemia [8][11].

The initial inflammatory profile of our patients (CRP > 6 mg/L: 46.67% of subjects; elevated alpha-glycoproteins: 42.66%; hypercomplementemia: 68%) is characteristic of the acute multifactorial inflammation associated with sickle cell disease. The pathophysiology of this inflammation is based on a vicious cycle involving: the polymerization of HbS leading to erythrocyte sickling, the adhesion of sickled erythrocytes to the vascular endothelium mediated by P-selectin, VCAM-1 (Vascular Cell Adhesion Molecule-1), and integrins, the activation of neutrophils and platelets, followed by the release of pro-inflammatory mediators and free radicals [5][6].

The hypercomplementemia observed in 68% of patients is explained by the fact that free hemin released during hemolysis interacts with complement molecules (C1q, CRP, immunoglobulins), notably activating the classical pathway and leading to increased cleavage of C4 and C3 [7]. This complement activation sustains endothelial inflammation and contributes to recurrent vascular occlusion.

CRP, synthesized by the liver under the influence of interleukin-6 (IL-6) and interleukin-1β (IL-1β), is a sensitive marker of systemic inflammation. In this study, 49.12% of malnourished patients had pathologically elevated CRP at enrollment, with a mean quantitative value of 12.77 (±2.35) mg/L. This level is consistent with a moderate chronic inflammatory state, which is consistent with the presence of recurrent vaso-occlusive episodes and frequent subclinical infections in this population [5][7]. Immune responses to these infections lead to hypergammaglobulinemia. Hypergammaglobulinemia (72%) reflects chronic stimulation of the adaptive immune system, secondary to repeated infections and autoimmunization linked to the release of erythrocyte antigens during chronic hemolysis [7].

Paired analysis using the McNemar test confirmed a significant improvement in CRP levels, with 22 patients moving from pathological to normal levels compared to only 3 in the opposite direction (p = 0.00016; paired OR = 7.33, 95% CI: 2.20 - 24.50), which is consistent with the expected anti-inflammatory effect of the intervention. In contrast, no significant change was observed for alpha-1-glycoprotein (p = 1.000; matched OR = 1.08, 95% CI: 0.51 - 2.29), with an almost equal number of patients showing improvement (n = 14) and worsening (n = 13).

Serum albumin showed the most marked improvement among all the protein parameters studied: 14 patients who were hypoalbuminemic at enrollment returned to normal levels at 6 months, compared with only 2 who showed the opposite trend (p = 0.00418; matched OR = 7.00, 95% CI: 1.59 - 30.80), reflecting a significant restoration of visceral protein status. It should be noted that albumin is not solely a nutritional marker: it is also a negative acute-phase protein, whose hepatic synthesis decreases under the influence of IL-6 in favor of positive acute-phase proteins such as CRP, and whose level can also be lowered by increased vascular permeability in an inflammatory context [49]. In sickle cell disease, where chronic undernutrition and systemic inflammation coexist and are mutually reinforcing, the concurrent improvement in CRP and albumin strengthens the biological coherence of the enriched flour’s combined anti-inflammatory and nutritional effect. Beta-2-glycoprotein showed a comparable favorable trend (27 patients normalized versus 5 who worsened), though it did not reach the threshold for statistical significance (p = 0.143; matched OR = 2.40, 95% CI: 0.85 - 6.81), which may reflect a lack of statistical power rather than an actual absence of effect.

This discrepancy among markers suggests that acute-phase proteins and visceral proteins do not respond uniformly or synchronously to the nutritional intervention, with CRP and albumin appearing to be more sensitive indicators of short-term changes in this population than alpha-1- and beta-2-glycoprotein. The significant reduction in inflammatory marker s after 6 months of intervention can be explained by the combined and synergistic effects of the bioactive compounds in the flour on the molecular pathways of inflammation.

Curcumin from Curcuma longais the most well-documented anti-inflammatory compound in the formulation. It exerts its effects by inhibiting the degradation of cytoplasmic Inhibitor of Nuclear Factor kappa-B alpha (IκBα) and by reducing the phosphorylation of the transcription factor nuclear factor kappa-B (p65/NF-κB), thereby blocking the transcription of numerous pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) [50]. At the same time, curcumin inhibits the Mitogen-Activated Protein Kinase (MAPK) pathways Extracellular Signal-Regulated Kinase 1 and 2 (ERK1/2), c-Jun N-terminal Kinase (JNK), and 38 kDa protein (p38), which are involved in the propagation of the inflammatory signal, and activates the Keap1-Nrf2/ARE pathway (Kelch-like ECH-Associated Protein 1/Nuclear factor Erythroid 2-related factor 2/Antioxidant Response Element), which strengthens endogenous antioxidant defenses (superoxide dismutase (SOD), catalase, glutathione) [50][51]. In mouse models of sickle cell disease, curcumin has been shown to significantly reduce hyperalgesia, hemolysis, oxidative stress, and inflammation (decreased levels of IL-2, IL-4, IL-6, and monocyte chemoattractant protein-1 (MCP-1)), as well as an improvement in hematological parameters, suggesting a disease-modifying effect [51].

Moringa oleiferaalso contributes to reducing inflammation by inhibiting the nuclear translocation of NF-κB and upregulating Nrf2, leading to a reduction in the expression of pro-inflammatory genes and an increase in the production of anti-inflammatory cytokines [52]. An umbrella review of 26 meta-analyses confirmed these effects, attributing moringa’s anti-inflammatory and antioxidant activity to its glucosinolates, flavonoids, and phenolic acids [53]. In in vitro models, Moringa oleiferaextracts at 4 mg/mL inhibited HbS sickling by 95.6% (±2.47%), with iron-chelating activity capable of reducing erythrocyte oxidative stress [54]. Moringa’s high content of antisickling amino acids (phenylalanine, lysine, arginine, histidine, and tryptophan) provides a complementary mechanism of action against HbS polymerization [54].

The baobab (Adansonia digitata) is an exceptional source of vitamin C (200 - 500 mg/100g, or 7 to 10 times the content of oranges), polyphenols (proanthocyanidins, flavonoids, catechins, tannins), and minerals (calcium, potassium, iron, magnesium, phosphorus, sodium, zinc, and manganese). Among these minerals, zinc is particularly relevant in sickle cell disease due to its documented antisickling activity and its role as a cofactor for superoxide dismutase (SOD), while iron helps correct the iron-deficiency anemia frequently associated with this condition [55][56]. These compounds exhibit antioxidant properties (neutralization of reactive oxygen species or free radicals, ROS), anti-inflammatory properties (inhibition of the lipoxygenase and xanthine oxidase pathways), antibacterial properties (bactericidal or bacteriostatic), and analgesic properties (pain-relieving) [55]. The high vitamin C content of baobab is particularly relevant in sickle cell disease, where erythrocyte oxidative stress is intense and reserves of water-soluble antioxidants are frequently insufficient [8].

The soy isoflavones (genistein, daidzein, glycitein) present in the soy component of the formulation are phytoestrogens with well-documented anti-inflammatory and antioxidant properties. They inhibit LPS-induced nitric oxide production (by inhibiting inducible nitric oxide synthase, or iNOS) and reduce pro-inflammatory cytokines (IL-6, IL-8, TNF-α, IL-12) in various types of immune cells [57][58]. Genistein, which has the highest antioxidant activity among isoflavones, outperforms ascorbate and alpha-tocopherol in protecting cells against oxidative stress. It also inhibits the MAPK/NF-κB pathways and activates the NRF2/HO-1 pathway [59].

Sesame contributes through its lignans (sesamin, sesamolin), which exert antioxidant effects by inhibiting lipid peroxidation and modulating antioxidant enzymes.

This synergistic combination of antioxidant and anti-inflammatory effects among the compounds in the enriched flour explains the extent of the reduction in inflammatory metabolites: pathological quantitative CRP levels decreased from 49.12% to 15.79% of patients (p = 0.0001), quantitative CRP levels decreased from 12.77 to 4.56 mg/L (64%).

The 86% reduction in VOCs is the most clinically significant result of this study. This association between improved inflammatory status and reduced VOCs is mechanistically consistent.

By reducing systemic inflammation (CRP, complement), the enriched flour attenuates endothelial activation and the overexpression of adhesion molecules. Furthermore, the reduction in erythrocyte oxidative stress (vitamins C and E from baobab and moringa, polyphenols, curcumin) improves erythrocyte deformability and reduces their propensity for sickling. This dual anti-inflammatory and antioxidant action is the most likely mechanism to explain the observed reduction in CVOs [5][60]. A study conducted in the Democratic Republic of the Congo involving 838 patients documented the relationship between inflammatory status and the frequency of CVOs in an African context, highlighting the importance of integrated care [11].

The improvement in normal muscle mass (+21%, from 45.61% to 57.89% of patients) and normal fat mass (+14%) reflects the anabolic effect of soy protein intake on muscle synthesis, combined with the energy provided by corn and the reduction in inflammatory hypercatabolism. These results are particularly significant in sickle cell disease, where muscle wasting is common and contributes to chronic fatigue and low exercise tolerance [8].

The normalization of bone quality (BMD) in 100% of patients at 6 months is a remarkable result, likely explained by the combined intake of calcium (baobab: 250 - 655 mg/100g), vitamin D, and protein (soy), as well as by the reduction of chronic inflammation, which promotes osteolysis via osteoclast activation mediated by the Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL) [8]. Soy isoflavones (genistein, daidzein), due to their estrogenic activity, may also contribute to the inhibition of bone resorption, as demonstrated in other contexts [59].

The increase in serum phosphorus levels (+11%) is consistent with improved intestinal absorption linked to overall nutritional status and a reduction in ischemic episodes in the intestine that contribute to malabsorption. Electrolyte levels (sodium, potassium, chloride) remained relatively stable, suggesting that the enriched flour did not disrupt the patients’ fluid and electrolyte balance.

The strengths of this study include: its prospective design with pre- and post-intervention assessments, the multimodal nature of the diagnostic tools used (anthropometry, laboratory tests, bioimpedance analysis), the use of a reproducible and accessible locally developed formulation, and the reasonable sample size for an African population with sickle cell disease.

The main limitations are the absence of a randomized control group (which does not allow for the formal exclusion of a seasonal effect or the impact of standard care on certain parameters). The interpretation of TSFT must be qualified due to the documented fat redistribution in patients with sickle cell disease [61][62] and the limitations of predictive equations when applied to general populations [63]. Furthermore, the lack of specific pro-inflammatory cytokine assays (TNF-α, IL-6, IL-1β) prevents a precise characterization of the predominant molecular mechanism of action of the flour. The kit utilization rate 43.43% for the initial cohort (n = 75) and 52.30% for the 57 patients who completed follow-up reflects moderate adherence, and the gap between the two groups suggests a selection bias favoring more adherent patients. This adherence level is likely explained by logistical constraints (distance to the center, patient availability), the daily burden of twice- or thrice-daily intake over six months, and progressive sensory fatigue: the initial hedonic evaluation had already shown only a moderate score (6.22 ± 1.55) [63]">[15]; and patients reported a decline in taste, smell, and color appreciation of the porridge by the end of follow-up. This low adherence represents a major limitation, likely to attenuate the true magnitude of the intervention’s effects, and calls for improvement strategies (closer support, organoleptic reformulation) in future similar interventions.