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  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">ns</journal-id>
      <journal-title-group>
        <journal-title>Natural Science</journal-title>
      </journal-title-group>
      <issn pub-type="epub">2150-4105</issn>
      <issn pub-type="ppub">2150-4091</issn>
      <publisher>
        <publisher-name>Scientific Research Publishing</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.4236/ns.2026.185006</article-id>
      <article-id pub-id-type="publisher-id">ns-151486</article-id>
      <article-categories>
        <subj-group>
          <subject>Article</subject>
        </subj-group>
        <subj-group>
          <subject>Biomedical</subject>
          <subject>Life Sciences</subject>
          <subject>Chemistry</subject>
          <subject>Materials Science</subject>
          <subject>Earth</subject>
          <subject>Environmental Sciences</subject>
          <subject>Medicine</subject>
          <subject>Healthcare</subject>
          <subject>Physics</subject>
          <subject>Mathematics</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>Giant Honeybee, Apis dorsata (Hymenoptera: Apidae) Colony Population during Summer Season amidst Urban Conditions of Mysore, Karnataka, India</article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" corresp="yes">
          <name name-style="western">
            <surname>Sekarappa</surname>
            <given-names>Basavarajappa</given-names>
          </name>
          <xref ref-type="aff" rid="aff1">1</xref>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Yaligar</surname>
            <given-names>Chetankumar</given-names>
          </name>
          <xref ref-type="aff" rid="aff1">1</xref>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Ramesh</surname>
            <given-names>Tarun Shrikaklum</given-names>
          </name>
          <xref ref-type="aff" rid="aff1">1</xref>
        </contrib>
        <contrib contrib-type="author">
          <name name-style="western">
            <surname>Anand</surname>
            <given-names>Suhasini Mysore</given-names>
          </name>
          <xref ref-type="aff" rid="aff1">1</xref>
        </contrib>
      </contrib-group>
      <aff id="aff1"><label>1</label> DOS in Zoology, University of Mysore, Mysore, India </aff>
      <author-notes>
        <fn fn-type="conflict" id="fn-conflict">
          <p>The authors declare no conflicts of interest regarding the publication of this paper.</p>
        </fn>
      </author-notes>
      <pub-date pub-type="epub">
        <day>22</day>
        <month>05</month>
        <year>2026</year>
      </pub-date>
      <pub-date pub-type="collection">
        <month>05</month>
        <year>2026</year>
      </pub-date>
      <volume>18</volume>
      <issue>05</issue>
      <fpage>73</fpage>
      <lpage>89</lpage>
      <history>
        <date date-type="received">
          <day>
          </day>
          <month>
          </month>
          <year>
          </year>
        </date>
        <date date-type="accepted">
          <day>
          </day>
          <month>
          </month>
          <year>
          </year>
        </date>
        <date date-type="published">
          <day>22</day>
          <month>05</month>
          <year>2026</year>
        </date>
      </history>
      <permissions>
        <copyright-statement>© 2026 by the authors and Scientific Research Publishing Inc.</copyright-statement>
        <copyright-year>2026</copyright-year>
        <license license-type="open-access">
          <license-p> This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">https://creativecommons.org/licenses/by/4.0/</ext-link> ). </license-p>
        </license>
      </permissions>
      <self-uri content-type="doi" xlink:href="https://doi.org/10.4236/ns.2026.185006">https://doi.org/10.4236/ns.2026.185006</self-uri>
      <abstract>
        <p>The Mysore (12.29′58˚N, 76.63′94˚E) lies at 770 meters above sea level at the base of Chamundi Hills and has several natural lakes such as Kukkarahalli, Karanji, Lingambudhi and known for salubrious climate with good vegetation. All these features have attracted many migratory animals including honeybee, <italic>Apis dorsata</italic> (Hymenoptera: Apidae) during different seasons. Systematic observations were made during summer season to record <italic>A. dorsata</italic> colony population by following random sampling method at diversified habitats from April to June, 2024. Total 164 nest sites were used by <italic>A. dorsata</italic> to establish colonies at man-made structures and tree species. Altogether, 829 <italic>A. dorsata</italic> colonies were recorded at different nesting sites, of which, 604 colonies were recorded on various man-made structures with 13 different coloured surfaces such as white, brown, yellow and grey colours along with glass surface. Total 225 colonies were recorded on different tree species at various heights ranged in between 10 to 110 ft and above the ground level. The colonies were grouped into small, medium, big size and had four shapes namely “U”, “V”, “irregular” and “dome” shape with different orientations. In Mysore, the prevailed conditions along with foraging source at the vicinity of <italic>A. dorsata</italic> colonies during summer season were good. Of all, Fabaceae family members were predominantly supplied floral source (both nectar and pollen) along with good supply of water from different source. Besides locally prevailed good conditions helped <italic>A. dorsata</italic>to have normal survival during summer season despite experiencing several biological constraints. Being an open nester, <italic>A. dorsata</italic>experiencing different levels of human interference, killing, burning, spraying pesticides on the colonies, hunting and pre-monsoon rainfall was observed during summer season. Despite various interferences, <italic>A. dorsata</italic> showed nest site fidelity to its previously nested site during summer in Mysore. <italic>A. dorsata</italic> is one of the potential pollinators, its hive products are economically important and <italic>A. dorsata</italic>playing a pivotal role in pollination and poverty alleviation as well. Hence, its investigation during different seasons helps reveal the status under natural conditions that could be used for local biodiversity conservation.</p>
      </abstract>
      <kwd-group kwd-group-type="author-generated" xml:lang="en">
        <kwd>Giant Honeybee</kwd>
        <kwd>&lt;i&gt;Apis&lt;/i&gt; &lt;i&gt;dorsata&lt;/i&gt;</kwd>
        <kwd>Colony Population</kwd>
        <kwd>Summer</kwd>
        <kwd>Mysore</kwd>
      </kwd-group>
    </article-meta>
  </front>
  <body>
    <sec id="sec1">
      <title>1. Introduction</title>
      <p>Human association existed with honeybees since pre-historic times and it was evidenced by observing the beekeeping activities in ancient Egyptian, Greek and Mesoamerican civilizations [[<xref ref-type="bibr" rid="B1">1</xref>]]. This long-standing relationship underscores the importance of honeybees not only as economic resources [[<xref ref-type="bibr" rid="B2">2</xref>]], but also as cultural, ecological and symbolic organisms. Of all the existing honeybee species, wild honeybee, <italic>Apis dorsata</italic>(Hymenoptera: Apidae) is one of the major pollinator species, and its hive products have good medicinal properties and are useful to mankind across the world [[<xref ref-type="bibr" rid="B3">3</xref>]]. Being a social insect [[<xref ref-type="bibr" rid="B4">4</xref>], [<xref ref-type="bibr" rid="B5">5</xref>]], it is an open nester [[<xref ref-type="bibr" rid="B6">6</xref>]-[<xref ref-type="bibr" rid="B8">8</xref>]], building nests on multistoried man-made establishments [[<xref ref-type="bibr" rid="B9">9</xref>], [<xref ref-type="bibr" rid="B10">10</xref>]], tall trees [[<xref ref-type="bibr" rid="B9">9</xref>], [<xref ref-type="bibr" rid="B11">11</xref>], [<xref ref-type="bibr" rid="B12">12</xref>]] and rock cliffs [[<xref ref-type="bibr" rid="B13">13</xref>]-[<xref ref-type="bibr" rid="B16">16</xref>]] to have safe survival, colony stability and help achieve good colony growth, seasonal swarming cycle and migration [[<xref ref-type="bibr" rid="B8">8</xref>], [<xref ref-type="bibr" rid="B17">17</xref>]]. Thus, <italic>A. dorsata</italic> inhabits not only in forested area and rural regions, but also was found abundantly in urban and semi-urban regions [[<xref ref-type="bibr" rid="B18">18</xref>], [<xref ref-type="bibr" rid="B19">19</xref>]]. Numerous <italic>A. dorsata</italic> colonies are observed in urban areas due to abundantly occurring man-made structures and tall avenue trees [[<xref ref-type="bibr" rid="B10">10</xref>], [<xref ref-type="bibr" rid="B15">15</xref>], [<xref ref-type="bibr" rid="B20">20</xref>], [<xref ref-type="bibr" rid="B21">21</xref>]]. In metropolitan city areas, nests are more likely to be found on buildings due to the increased protection from the sun, wind and rain [[<xref ref-type="bibr" rid="B22">22</xref>]]. However, <italic>A. dorsata</italic>colony population showed seasonal fluctuations, with the greater number of colonies found in winter followed by summer seasons. <bold>Table 1</bold> shows few published reports on <italic>A. dorsata</italic> at different parts of the world. Various researchers have recorded the nesting behavior of <italic>A. dorsata</italic>at different parts of India and other parts of the world including Karnataka [[<xref ref-type="bibr" rid="B23">23</xref>], [<xref ref-type="bibr" rid="B24">24</xref>]]. Sixty colonies of <italic>A. dorsata</italic> at south Andaman Island, India was recorded by [[<xref ref-type="bibr" rid="B25">25</xref>]]. <italic>A. dorsata</italic> colonies on 15 trees in Subah, Borneo, Indonesia were recorded by [[<xref ref-type="bibr" rid="B26">26</xref>]]. <italic>A. dorsata</italic> nests elevation at 51 nesting sites by studying 1,434 nests in and around Bangalore, Karnataka was measured by [[<xref ref-type="bibr" rid="B27">27</xref>]]. Few nesting parameters of <italic>A. dorsata</italic> in Raichur, Karnataka were recorded by [[<xref ref-type="bibr" rid="B28">28</xref>]]. Nests distributions of <italic>A. dorsata</italic>at 11 sites in and around Bangalore were reported by [[<xref ref-type="bibr" rid="B13">13</xref>]]. Seasonal migration of <italic>A. dorsata</italic> in protected habitat of Bogor Botanical Garden, West Jawa, Indonesia by monitoring the arrivals and departures of colonies was observed by [[<xref ref-type="bibr" rid="B29">29</xref>]]. Further, migratory behavior of <italic>A. dorsata</italic>was studied by [[<xref ref-type="bibr" rid="B30">30</xref>]]. Nesting behaviour and conservation status of <italic>A. dorsata</italic>, in southern Deccan Peninsula, Karnataka was reported by [[<xref ref-type="bibr" rid="B31">31</xref>], [<xref ref-type="bibr" rid="B32">32</xref>]]. Furthermore, nesting behavior, elevation and orientation of<italic>A. dorsata</italic>from 197 colonies at CCS Haryana Agricultural University, Hisar, Haryana was studied by [[<xref ref-type="bibr" rid="B33">33</xref>]]. Similarly, [[<xref ref-type="bibr" rid="B21">21</xref>], [<xref ref-type="bibr" rid="B22">22</xref>]] have studied the nesting behaviour of <italic>A. dorsata</italic> in Mysore. [[<xref ref-type="bibr" rid="B20">20</xref>]] has studied the colony density variation in <italic>A. dorsata</italic> during different seasons. [[<xref ref-type="bibr" rid="B10">10</xref>]] has studied biological constraints affecting <italic>A. dorsata</italic> in southern Karnataka. [[<xref ref-type="bibr" rid="B8">8</xref>]] has studied 587<italic>A. dorsata</italic> colonies from 23 nesting sites in Nepal, India and Bhutan. [[<xref ref-type="bibr" rid="B18">18</xref>]] has recorded 2,407 live colonies of <italic>A. dorsata</italic>on trees, rock cliffs and man-made structures in southern Karnataka. [[<xref ref-type="bibr" rid="B34">34</xref>]] has recorded the nest orientation of <italic>A. dorsata</italic> colonies on different objects at plains of south Karnataka. [[<xref ref-type="bibr" rid="B16">16</xref>]] has recorded more number of <italic>A. dorsata</italic> colonies on man-made buildings and trees in urban regions of Bengaluru, Karnataka. </p>
      <p>However, in recent past, honeybee population is seriously threatened by deforestation, agricultural intensification, monoculture farming, extensive pesticide use, destructive honey hunting activities, forest fires, prolonged droughts, increasing temperature and regional/local climate change [[<xref ref-type="bibr" rid="B35">35</xref>], [<xref ref-type="bibr" rid="B36">36</xref>]] at different parts of the world. Hence, honeybee population is facing unprecedented threats due to various reasons. Since, honeybees represent a biologically diverse and ecologically indispensable group of insects, whose survival is tightly linked to global food security, biodiversity [[<xref ref-type="bibr" rid="B2">2</xref>], [<xref ref-type="bibr" rid="B37">37</xref>]] and ecosystem resilience. Moreover, honeybees (e.g. <italic>A</italic><italic>. dorsata</italic>) are potential pollinators, recording that their colony population requires continuous monitoring during different seasons to monitor local/regional biodiversity. However, published reports on <italic>A. dorsata</italic> during summer season amidst urban conditions are incomplete. Therefore, understanding their distribution, colony population and foraging sources during different seasons is essential for developing effective conservation [[<xref ref-type="bibr" rid="B24">24</xref>], [<xref ref-type="bibr" rid="B38">38</xref>], [<xref ref-type="bibr" rid="B39">39</xref>]] and management strategies. Hence, present study was undertaken during </p>
      <p><bold>Table 1.</bold><bold>Few published reports on giant honeybee,</bold><italic><bold>Apis dorsata</bold></italic><bold>in India and other parts of the world</bold><bold>.</bold></p>
      <table-wrap id="tbl1">
        <label>Table 1</label>
        <table>
          <tbody>
            <tr>
              <td>Sl.No.</td>
              <td>Researched on</td>
              <td>Important findings</td>
              <td>Place</td>
              <td>Source</td>
            </tr>
            <tr>
              <td>1.</td>
              <td>Nesting behaviour of Indian honeybee</td>
              <td>Recorded different nest sites of honeybees</td>
              <td>India</td>
              <td>
                Deodikar
                <italic>et al.</italic>
                (1977)
              </td>
            </tr>
            <tr>
              <td>2.</td>
              <td>Retrieval of visuo-spatial memories by honeybees</td>
              <td>Identified the selection of nest sites</td>
              <td>-</td>
              <td>Collette and Kelber (1988)</td>
            </tr>
            <tr>
              <td>3.</td>
              <td>
                Height-dependent nest site selection in
                <italic>Apis dorsata</italic>
              </td>
              <td>Recorded the height-dependent nesting activities</td>
              <td>Bangalore, India</td>
              <td>Reddy and Reddy (1989)</td>
            </tr>
            <tr>
              <td>4.</td>
              <td>
                Colony migration in the tropical honeybee,
                <italic>Apis dorsata</italic>
              </td>
              <td>Recorded migratory behaviour</td>
              <td>India</td>
              <td>Dyer and Seeley (1994)</td>
            </tr>
            <tr>
              <td>5.</td>
              <td>Arboreal bee forage plants</td>
              <td>Identified epiphytes floral source to honeybees</td>
              <td>India</td>
              <td>
                Joshi
                <italic>et al.</italic>
                (1998)
              </td>
            </tr>
            <tr>
              <td>6.</td>
              <td>
                Status of natural colonies of
                <italic>Apis dorsata</italic>
              </td>
              <td>Recorded the status</td>
              <td>Karnataka, India</td>
              <td>Basavarajappa (1998)</td>
            </tr>
            <tr>
              <td>7.</td>
              <td>Honeybee memory: Navigation by associative grouping and recall of visual stimuli.</td>
              <td>Observed the associative grouping and recall of visual stimuli by honeybees</td>
              <td>-</td>
              <td>
                Zhang
                <italic>et al.</italic>
                (1999)
              </td>
            </tr>
            <tr>
              <td>8.</td>
              <td>
                Nesting site recognition by
                <italic>A. dorsata</italic>
              </td>
              <td>Identified different nest sites</td>
              <td>China</td>
              <td>
                Liu
                <italic>et al</italic>
                . (2000)
              </td>
            </tr>
            <tr>
              <td>9.</td>
              <td>
                Defence strategies in
                <italic>A. dorsata</italic>
                under natural conditions
              </td>
              <td>Recorded different defence strategies against pests and predators</td>
              <td>-</td>
              <td>
                Kastberger
                <italic>et al.</italic>
                (2001)
              </td>
            </tr>
            <tr>
              <td>10.</td>
              <td>Memory use in insect visual navigation</td>
              <td>Recorded how insects use navigation by visual cues</td>
              <td>-</td>
              <td>Collette and Collette (2002)</td>
            </tr>
            <tr>
              <td>11.</td>
              <td>Home-site fidelity in migratory honeybees</td>
              <td>Recorded the home-site fidelity in honeybees</td>
              <td>-</td>
              <td>
                Neumann
                <italic>et al.</italic>
                (2000)
              </td>
            </tr>
            <tr>
              <td>12.</td>
              <td>Giant honeybees return to their nest sites</td>
              <td>Observed the return of honeybees to their previously nested sites</td>
              <td>-</td>
              <td>
                Paar
                <italic>et al</italic>
                . (2000)
              </td>
            </tr>
            <tr>
              <td>13.</td>
              <td>Grouping of visual objects of honeybees</td>
              <td>Recorded the objects which are used for nesting</td>
              <td>-</td>
              <td>
                Zang
                <italic>et al.</italic>
                (2004)
              </td>
            </tr>
            <tr>
              <td>14.</td>
              <td>
                Status of
                <italic>Apis dorsata</italic>
              </td>
              <td>Recorded the natural status</td>
              <td>Karnataka, India</td>
              <td>Basavarajappa (2004)</td>
            </tr>
            <tr>
              <td>15.</td>
              <td>Group decision making in nest-site selection by honeybees</td>
              <td>Recorded the group decision making in nest-site selection</td>
              <td>-</td>
              <td>Seeley and Visscher (2004)</td>
            </tr>
            <tr>
              <td>16.</td>
              <td>Honeybee memory recorded experimentally</td>
              <td>Recorded the honeybee decision making abilities</td>
              <td>China</td>
              <td>
                Zang
                <italic>et al</italic>
                . (2006)
              </td>
            </tr>
            <tr>
              <td>17.</td>
              <td>
                Nesting behaviour of
                <italic>Apis dorsata</italic>
              </td>
              <td>Identified different nest sites</td>
              <td>North India</td>
              <td>
                Khan
                <italic>et al.</italic>
                (2007)
              </td>
            </tr>
            <tr>
              <td>18.</td>
              <td>
                Old comb for nesting site recognition by
                <italic>Apis dorsata</italic>
                ?
              </td>
              <td>
                Recorded how
                <italic>A. dorsata</italic>
                identify the previously nested sites
              </td>
              <td>China</td>
              <td>
                Liu
                <italic>et al</italic>
                . (2007)
              </td>
            </tr>
            <tr>
              <td>19.</td>
              <td>
                Nesting behaviour of
                <italic>A. dorsata</italic>
              </td>
              <td>Recorded nesting behaviour</td>
              <td>Mysore, India</td>
              <td>Manjunath (2008)</td>
            </tr>
            <tr>
              <td>20.</td>
              <td>
                Pests of
                <italic>Apis dorsata</italic>
              </td>
              <td>Identified the pests</td>
              <td>Karnataka</td>
              <td>Basavarajappa (2009)</td>
            </tr>
            <tr>
              <td>21.</td>
              <td>
                Characteristics of trees used as nest sites by
                <italic>Apis dorsata</italic>
              </td>
              <td>
                Recorded trees as nest sites of
                <italic>Apis dorsata</italic>
              </td>
              <td>Nilgiri Biosphere Reserve, India</td>
              <td>
                Thomas
                <italic>et al</italic>
                . (2009)
              </td>
            </tr>
            <tr>
              <td>22.</td>
              <td>Bee tree of Sahyadri</td>
              <td>
                Recorded 75
                <italic>A. dorsata</italic>
                colonies
              </td>
              <td>Shimogga, India</td>
              <td>Basavarajappa (2009)</td>
            </tr>
            <tr>
              <td>23.</td>
              <td>Honeybees and the uses to mankind</td>
              <td>Revealed usefulness of honeybees to ecosystem</td>
              <td>India</td>
              <td>Basavarajappa (2010)</td>
            </tr>
            <tr>
              <td>24.</td>
              <td>Anthropogenic interferences on wild honeybees</td>
              <td>Recorded various man-made activities impact on wild honeybees</td>
              <td>Mysore, India</td>
              <td>Basavarajappa (2010)</td>
            </tr>
            <tr>
              <td>25.</td>
              <td>
                Nest host trees of
                <italic>Apis dorsata</italic>
              </td>
              <td>Identified the bee trees in southern Karnataka</td>
              <td>India</td>
              <td>Basavarajappa (2009)</td>
            </tr>
            <tr>
              <td>26.</td>
              <td>Conservation of Asian honeybees</td>
              <td>Suggested the conservation of honeybees</td>
              <td>-</td>
              <td>Oldroyd and Nanork (2009)</td>
            </tr>
            <tr>
              <td>27.</td>
              <td>
                Colony status of
                <italic>Apis dorsata</italic>
              </td>
              <td>Identified the colony status</td>
              <td>Southern Karnataka</td>
              <td>Basavarajappa and Raghunandan (2013)</td>
            </tr>
            <tr>
              <td>28.</td>
              <td>
                Greater wax moth
                <italic>, Galleria mellonella</italic>
                infestation on
                <italic>Apis dorsata</italic>
              </td>
              <td>
                Recorded
                <italic>G. mellonella</italic>
                infestation
              </td>
              <td>South-western Karnataka, India</td>
              <td>Raghunandan and Basavarajappa (2014)</td>
            </tr>
            <tr>
              <td>29.</td>
              <td>
                Floral hosts of
                <italic>Apis dorsata</italic>
              </td>
              <td>
                Recorded various flowering plants which provide floral source to
                <italic>A. dorsata</italic>
              </td>
              <td>Southern Karnataka, India</td>
              <td>Raghunandan and Basavarajappa (2014)</td>
            </tr>
            <tr>
              <td>30.</td>
              <td>
                Bio-ecology of
                <italic>Apis dorsata</italic>
              </td>
              <td>
                Recorded the influence of biological and ecological factors on the survival of
                <italic>A. dorsata</italic>
              </td>
              <td>Southern Karnataka, India</td>
              <td>Raghunandan and Basavarajappa (2014)</td>
            </tr>
            <tr>
              <td>31.</td>
              <td>Honeybee diversity is precious</td>
              <td>Essential to preserve local biodiversity</td>
              <td>India</td>
              <td>Basavarajappa (2018)</td>
            </tr>
            <tr>
              <td>32.</td>
              <td>Conservation of honeybees</td>
              <td>Essential for human survival is highlighted</td>
              <td>India</td>
              <td>Basavarajappa (2020)</td>
            </tr>
            <tr>
              <td>33.</td>
              <td>Nesting ecology of honeybees under managed and natural landscapes</td>
              <td>Highlighted the need for conservation of honeybees</td>
              <td>India</td>
              <td>Basavarajappa (2022)</td>
            </tr>
          </tbody>
        </table>
      </table-wrap>
      <p>summer season at Mysore city, India.</p>
    </sec>
    <sec id="sec2">
      <title>2. Materials and methods</title>
      <p><bold>Study</bold><bold>area:</bold>Mysore city is situated in the southern Deccan Plateau, lies at 12.29′58˚N, 76.63′94˚E with an elevation 770 meters above sea level. The city lies at the base of Chamundi Hills and have several natural lakes like Kukkarahalli, Karanji and Lingambudhi [[<xref ref-type="bibr" rid="B40">40</xref>]]. The city covers an area 155 km<sup>2</sup> to 286 km<sup>2</sup> with fertile landscape and possesses a salubrious climate with temperatures ranging from 15˚C (winter) to 35˚C (summer), supporting scrub jungle vegetation in uncultivated areas. March to end of May month’s experience summer season and it is followed by the south-west monsoon lasting up to September end. The October and November months are experiencing retreating monsoon. Moreover, the vegetation is thorn-scrubby type along with tropical forest of deciduous type and evergreen type is also noticed in the eastern portion of the district. All these prevailed conditions amidst Mysore district have provided suitable habitat for many migratory insects including different species of honeybees.</p>
      <p><bold>Methodology:</bold> Observation was made at randomly selected 40 study sites in Mysore city to record the <italic>A.</italic><italic>dorsata</italic> colony population during summer season as per [[<xref ref-type="bibr" rid="B15">15</xref>]]. Study sites represented the recreation parks, residential areas, school-college zones, office/administrative areas, commercial areas, religious places, hospital/health care centers, un-used barren fields, protected areas and at these sites, local people were met personally and collected information on <italic>A.</italic><italic>dorsata</italic> using pre-tested questionnaire that included more than 15 parameters. Further, study sites were earmarked with identification tags and visited once in a week from morning 8.00 AM to 12.00 Noon and evening 4.00 PM to 7.00 PM for a period of three months <italic>i.e.</italic>, from April to June, 2024 continuously. <italic>A.</italic><italic>dorsata</italic> colonies were observed by following an all-out search method, where every spot is critically surveyed and checked to record the <italic>A.</italic><italic>dorsata</italic> colonies at various man-made structures, on trees and on rock cliffs using binocular, digital camera and through naked eyes as per [[<xref ref-type="bibr" rid="B9">9</xref>], [<xref ref-type="bibr" rid="B12">12</xref>], [<xref ref-type="bibr" rid="B15">15</xref>]]. Live or deserted colonies, colony nested place, number of colonies, name of nest host tree, size and shape of the colony, nest orientation, colour of the nested surfaced objects and locally available resources nearby the nest, water source, human interference and nest site fidelity were recorded as per [[<xref ref-type="bibr" rid="B10">10</xref>], [<xref ref-type="bibr" rid="B41">41</xref>]-[<xref ref-type="bibr" rid="B43">43</xref>]]. Nesting architecture was studied carefully by opening deserted nests to observe brood cells, pollen chambers, honey chambers and internal nest arrangement as per [[<xref ref-type="bibr" rid="B9">9</xref>]]. Floral source was recorded as per [[<xref ref-type="bibr" rid="B44">44</xref>]]. Collected data was systematically analyzed, compiled using SPSS to find out the deviation and coefficient variation if any between the nest sites and colony parameters as per [[<xref ref-type="bibr" rid="B45">45</xref>]].</p>
    </sec>
    <sec id="sec3">
      <title>3. Results</title>
      <p><bold>Nest</bold><bold>sites:</bold> Total 164 nest sites were recorded at man-made structures and on different tree species during summer season in Mysore (<bold>Table 2</bold>). Of all, 15 sites were preferred on different tree species by <italic>A.</italic><italic>dorsata</italic> and remaining 149 sites were recorded on various man-made structures (<bold>Table 2</bold>). Amongst the man-made structures, commercial buildings were more preferred (45 sites) and it was followed by educational buildings (27 sites), residential buildings (22 sites), government offices and water tanks (15 sites each) preferred for nesting by <italic>A.</italic><italic>dorsata</italic> during summer season in Mysore. Moreover, other man-made structures included hotels, hospitals, conventional halls, religious and heritage buildings were also preferred for nest establishment by <italic>A.</italic><italic>dorsata</italic> and there existed a considerable coefficient variation (CV = 86.58) between them (<bold>Table 2</bold>).</p>
      <p><bold>No.</bold><bold>of</bold><bold>colonies:</bold> Total 829 <italic>A.</italic><italic>dorsata</italic> colonies were recorded at different nesting sites during summer season in Mysore. Of all, highest (604) colonies were recorded on various man-made structures (<bold>Table 2</bold>) and around 225 colonies were recorded on different tree species and showed considerable coefficient variation (CV = 89.32) between them during summer season. Distribution of <italic>A.</italic><italic>dorsata</italic> colonies on different man-made structures including trees are given in <bold>Table 2</bold>.</p>
      <p><bold>Nest</bold><bold>elevation:</bold><italic>A.</italic><italic>dorsata</italic> preferred to nest at various heights from the ground and it was ranged in between 10 to 110 ft and above the ground level during summer season (<bold>Table 2</bold>). Interestingly, 40 - 49 ft elevation was more preferred height, where highest (279) number of colonies were observed and it was followed by 30 - 39 and 20 - 29 feet height preferred by 242 and 212 colonies respectively (<bold>Table 2</bold>). However, at 10 - 19, 50 - 59, 60 - 69, 70 - 79, 80 - 89, 90 - 99 and 100 to 110 feet and above height was also preferred by <italic>A.</italic><italic>dorsata</italic> for their colonies establishment and exhibited considerable coefficient variation (CV = 84.45) during summer season in Mysore (<bold>Table 2</bold>).</p>
      <p><bold>Table 2.</bold><bold>Recorded few nesting parameters of giant honeybee,</bold><italic><bold>Apis dorsata</bold></italic><bold>during summer season in Mysore</bold><bold>.</bold></p>
      <table-wrap id="tbl2">
        <label>Table 2</label>
        <table>
          <tbody>
            <tr>
              <td>Sl.No.</td>
              <td>Name of nest site</td>
              <td>No. of nest sites</td>
              <td>No. of colonies</td>
              <td>Sl.No.</td>
              <td>Nest height (in ft)</td>
              <td>No. of colonies</td>
              <td>Sl.No.</td>
              <td>Nesting surface colour</td>
              <td>No. of colonies</td>
            </tr>
            <tr>
              <td>1.</td>
              <td>Educational Buildings</td>
              <td>27</td>
              <td>120</td>
              <td>1.</td>
              <td>0 - 9</td>
              <td>-</td>
              <td>1.</td>
              <td>White</td>
              <td>205</td>
            </tr>
            <tr>
              <td>2.</td>
              <td>Hotels</td>
              <td>10</td>
              <td>33</td>
              <td>2.</td>
              <td>10 - 19</td>
              <td>3</td>
              <td>2.</td>
              <td>Cream</td>
              <td>32</td>
            </tr>
            <tr>
              <td>3.</td>
              <td>Commercial Buildings</td>
              <td>45</td>
              <td>136</td>
              <td>3.</td>
              <td>20 - 29</td>
              <td>112</td>
              <td>3.</td>
              <td>Yellow</td>
              <td>73</td>
            </tr>
            <tr>
              <td>4.</td>
              <td>Government Offices</td>
              <td>15</td>
              <td>112</td>
              <td>4.</td>
              <td>30 - 39</td>
              <td>242</td>
              <td>4.</td>
              <td>Orange</td>
              <td>16</td>
            </tr>
            <tr>
              <td>5.</td>
              <td>Religious Buildings</td>
              <td>1</td>
              <td>4</td>
              <td>5.</td>
              <td>40 - 49</td>
              <td>279</td>
              <td>5.</td>
              <td>Red</td>
              <td>37</td>
            </tr>
            <tr>
              <td>6.</td>
              <td>Hospitals</td>
              <td>9</td>
              <td>74</td>
              <td>6.</td>
              <td>50 - 59</td>
              <td>80</td>
              <td>6.</td>
              <td>R-M</td>
              <td>37</td>
            </tr>
            <tr>
              <td>7.</td>
              <td>Residential Buildings</td>
              <td>22</td>
              <td>65</td>
              <td>7.</td>
              <td>60 - 69</td>
              <td>47</td>
              <td>7.</td>
              <td>Pink</td>
              <td>7</td>
            </tr>
            <tr>
              <td>8.</td>
              <td>Conventional Halls</td>
              <td>4</td>
              <td>6</td>
              <td>8.</td>
              <td>70 - 79</td>
              <td>12</td>
              <td>8.</td>
              <td>Blue</td>
              <td>7</td>
            </tr>
            <tr>
              <td>9.</td>
              <td>Heritage Buildings</td>
              <td>1</td>
              <td>6</td>
              <td>9.</td>
              <td>80 - 89</td>
              <td>10</td>
              <td>9.</td>
              <td>Green</td>
              <td>12</td>
            </tr>
            <tr>
              <td>10.</td>
              <td>Water Tanks</td>
              <td>15</td>
              <td>48</td>
              <td>10.</td>
              <td>90 - 99</td>
              <td>27</td>
              <td>10.</td>
              <td>Orange-Green</td>
              <td>2</td>
            </tr>
            <tr>
              <td rowspan="5">11.</td>
              <td rowspan="5">Trees</td>
              <td rowspan="5">15</td>
              <td rowspan="5">225</td>
              <td rowspan="5">11.</td>
              <td rowspan="5">100 - 110 &amp; above</td>
              <td rowspan="5">17</td>
              <td>11.</td>
              <td>Brown</td>
              <td>78</td>
            </tr>
            <tr>
              <td>12.</td>
              <td>Grey</td>
              <td>56</td>
            </tr>
            <tr>
              <td>13.</td>
              <td>Black</td>
              <td>22</td>
            </tr>
            <tr>
              <td>14.</td>
              <td>Silver</td>
              <td>10</td>
            </tr>
            <tr>
              <td>15.</td>
              <td>Glass</td>
              <td>10</td>
            </tr>
            <tr>
              <td colspan="2">Total ± SD</td>
              <td>164 ± 12.91</td>
              <td>829 ± 67.31</td>
              <td colspan="2">Total</td>
              <td>829 ± 98.16</td>
              <td colspan="2">Total</td>
              <td>604 ± 50.59</td>
            </tr>
            <tr>
              <td colspan="2">CV</td>
              <td>86.58</td>
              <td>89.32</td>
              <td colspan="2">-</td>
              <td>84.45</td>
              <td colspan="2">-</td>
              <td>92.13</td>
            </tr>
          </tbody>
        </table>
      </table-wrap>
      <p>Note: Each value is total of 12 observations. CV: Coefficient variability.</p>
      <p><bold>Colour</bold><bold>of</bold><bold>nested</bold><bold>surface:</bold><italic>A.</italic><italic>dorsata</italic> colonies were observed on 13 different coloured surfaces on various man-made structures (<bold>Table 2</bold>). Amongst all the colours, white colour was most commonly preferred for 205 colonies establishment during summer season by <italic>A.</italic><italic>dorsta</italic> (<bold>Table 2</bold>). The other coloured surface preferred by <italic>A.</italic><italic>dorsata</italic> were brown, yellow and grey colours where, 78, 73 and 56 colonies established respectively (<bold>Table 2</bold>). Moreover, other coloured surfaces on which <italic>A.</italic><italic>dorsata</italic> established their colonies are depicted in <bold>Table 2</bold>. Further, glass surface was also chosen by <italic>A.</italic><italic>dorsata</italic> for establishing its colonies and exhibited considerable coefficient variation (CV = 92.13) during summer season at Mysore (<bold>Table 2</bold>).</p>
      <p><bold>Colony conditions:</bold>Total 485 live colonies and 344 deserted colonies were recorded and there exhibited little coefficient variation (CV = 24.05) between them during summer season in Mysore (<bold>Table 3</bold>). The live colonies were enriched with workers, drones and queen bee. While, deserted colonies were devoid of workers, drones and queen bee. Among the deserted colonies, few were dismantled, many were torn and few were infested with wax moth. </p>
      <p><bold>Size</bold><bold>of</bold><bold>colonies</bold>: <italic>A.</italic><italic>dorsata</italic> established different sized colonies such as small, medium and big size respectively 180, 418 and 231 colonies during summer season in Mysore (<bold>Table 3</bold>). Interestingly, there was a moderate coefficient variation (CV = 45.35) existed between colony sizes during summer season.</p>
      <p><bold>Table 3.</bold><bold>Colony parameters of giant honeybee,</bold><italic><bold>Apis dorsata</bold></italic><bold>during summer season in Mysore</bold><bold>.</bold></p>
      <table-wrap id="tbl3">
        <label>Table 3</label>
        <table>
          <tbody>
            <tr>
              <td>Sl.No.</td>
              <td>Colonycondition</td>
              <td>No. ofcolonies</td>
              <td>Sl.No.</td>
              <td>Size of the colony</td>
              <td>No. of colonies</td>
              <td>Sl.No.</td>
              <td>Shape of the colony</td>
              <td>No. of colonies</td>
              <td>Sl.No.</td>
              <td>Orientation of the colony</td>
              <td>No. of colonies</td>
            </tr>
            <tr>
              <td>1.</td>
              <td>Live</td>
              <td>485</td>
              <td>1.</td>
              <td>Small</td>
              <td>180</td>
              <td>1.</td>
              <td>‘U’</td>
              <td>673</td>
              <td>1.</td>
              <td>North-south</td>
              <td>295</td>
            </tr>
            <tr>
              <td rowspan="3">2.</td>
              <td rowspan="3">Deserted</td>
              <td rowspan="3">344</td>
              <td>2.</td>
              <td>Medium</td>
              <td>418</td>
              <td>2.</td>
              <td>‘V’</td>
              <td>39</td>
              <td>2.</td>
              <td>East-west</td>
              <td>257</td>
            </tr>
            <tr>
              <td rowspan="2">3.</td>
              <td rowspan="2">Big</td>
              <td rowspan="2">231</td>
              <td>3.</td>
              <td>Irregular</td>
              <td>70</td>
              <td>3.</td>
              <td>Northeast-southwest</td>
              <td>178</td>
            </tr>
            <tr>
              <td>4.</td>
              <td>Dome</td>
              <td>47</td>
              <td>4.</td>
              <td>Northwest-southeast</td>
              <td>99</td>
            </tr>
            <tr>
              <td colspan="2">Total ± SD</td>
              <td>829 ± 99.70</td>
              <td colspan="2">Total</td>
              <td>829 ± 125.31</td>
              <td colspan="2">Total</td>
              <td>829 ±310.78</td>
              <td colspan="2">Total</td>
              <td>829 ±87.08</td>
            </tr>
            <tr>
              <td colspan="2">CV</td>
              <td>24.05</td>
              <td colspan="2">-</td>
              <td>45.35</td>
              <td colspan="2">-</td>
              <td>149.95</td>
              <td colspan="2">-</td>
              <td>42.02</td>
            </tr>
          </tbody>
        </table>
      </table-wrap>
      <p>Note: Each value is a total of 12 observations. CV: Coefficient variability.</p>
      <p><bold>Shape of colonies</bold>: During the present study, four shaped colonies were observed commonly. They were “U”, “V”, “irregular” and “dome” shaped colonies and shown highest coefficient variation (CV = 149.95) between them. Of all, “U” shaped colonies were more predominant (673 colonies) compared to others (156 colonies) and representing 81.2 and 18.8% respectively during summer season in Mysore (<bold>Table 3</bold>). </p>
      <p><bold>Orientation</bold>: <italic>A.</italic><italic>dorsata</italic> usually establish its colonies with different orientations under natural conditions. Being an open nester, perhaps to avail sunlight and to have good navigation path, <italic>A.</italic><italic>dorsata</italic> established differently oriented colonies at various man-made structures and on trees during summer season. <italic>A.</italic><italic>dorsata</italic> established north-south, east-west, northeast-southwest and northwest-south easterly oriented colonies and exhibited little coefficient variation (CV = 42.02) between the orientations (<bold>Table 3</bold>). Of all, north-southerly oriented <italic>A.</italic><italic>dorsata</italic> colonies were more (295 colonies) compared to other orientations during summer season in Mysore (<bold>Table 3</bold>).</p>
      <p>Further, <bold>Table 4</bold> shows few prevailed conditions at the vicinity of <italic>A.</italic><italic>dorsata</italic> colonies during summer season in Mysore. Around 604 <italic>A.</italic><italic>dorsata</italic> colonies were distributed on various man-made structures and exhibited considerable coefficient variation (CV = 64.65) between the man-made structures and trees. Only, 225 <italic>A. dorsata</italic> colonies were established on seven tree species. The <italic>Ceiba petandra, Ficus benghalensis</italic>, <italic>Albizia saman, Ficus religosa, Pterogyta allata, Peltophorum pterocarpum</italic> and <italic>Palm</italic> species were selected for nesting and shown highest coefficient variation (CV = 102.83) between the nested tree species. <xref ref-type="fig" rid="fig1">Figure 1</xref> shows the per cent occurrence of <italic>A.</italic><italic>dorsata</italic> colonies on different tree species. Comparatively, <italic>C.</italic><italic>petandra,</italic><italic>F.</italic><italic>benghalensis</italic> and <italic>A.</italic><italic>saman</italic> were more opted by <italic>A.</italic><italic>dorsata</italic> during summer season in Mysore. Number of <italic>A.</italic><italic>dorsata</italic> colonies established on different tree species are given in <bold>Table 4</bold>. Further, there was a good vegetation observed nearby 143 <italic>A.</italic><italic>dorsata</italic> colonies and source of water was good nearby 155 <italic>A.</italic><italic>dorsata</italic> colonies and showed less coefficient variation (CV = 5.69). <xref ref-type="fig" rid="fig2">Figure 2</xref> shows the foraging source to <italic>A.</italic><italic>dorsata</italic> during</p>
      <p><bold>Table 4.</bold><bold>Prevailed conditions at the vicinity of giant honeybee,</bold><italic><bold>Apis dorsata</bold></italic><bold>colonies during summer season in Mysore</bold><bold>.</bold></p>
      <table-wrap id="tbl4">
        <label>Table 4</label>
        <table>
          <tbody>
            <tr>
              <td>Sl.No.</td>
              <td>Nests observed on</td>
              <td>No. ofcolonies</td>
              <td>Sl.No.</td>
              <td>Nest host tree</td>
              <td>No. of colonies</td>
              <td>Sl.No.</td>
              <td>Natural resourceprevailed</td>
              <td>No. of colonies</td>
              <td>Sl.No.</td>
              <td>Source of water</td>
              <td>No. of colonies</td>
            </tr>
            <tr>
              <td>1.</td>
              <td>Man-made structures</td>
              <td>604</td>
              <td>1.</td>
              <td>
                <italic>Ceiba petandra</italic>
              </td>
              <td>78</td>
              <td>1.</td>
              <td>Vegetation</td>
              <td>143</td>
              <td>1.</td>
              <td>Tap</td>
              <td>74</td>
            </tr>
            <tr>
              <td>2.</td>
              <td>Rocks</td>
              <td>-</td>
              <td>2.</td>
              <td>
                <italic>Ficus benghalensis</italic>
              </td>
              <td>62</td>
              <td rowspan="6">2.</td>
              <td rowspan="6">Water</td>
              <td rowspan="6">155</td>
              <td>2.</td>
              <td>Water leakage from the pipe</td>
              <td>42</td>
            </tr>
            <tr>
              <td rowspan="5">3.</td>
              <td rowspan="5">Trees</td>
              <td rowspan="5">225</td>
              <td>3.</td>
              <td>
                <italic>Albizia saman</italic>
              </td>
              <td>58</td>
              <td>3.</td>
              <td>Stagnant water</td>
              <td>21</td>
            </tr>
            <tr>
              <td>4.</td>
              <td>
                <italic>Ficus religosa</italic>
              </td>
              <td>22</td>
              <td>4.</td>
              <td>Water tank</td>
              <td>17</td>
            </tr>
            <tr>
              <td>5.</td>
              <td>
                <italic>Pterogyta allata</italic>
              </td>
              <td>3</td>
              <td rowspan="3">5.</td>
              <td rowspan="3">Pond</td>
              <td rowspan="3">1</td>
            </tr>
            <tr>
              <td>6.</td>
              <td>
                <italic>Peltophorum</italic>
                <italic>pte</italic>
                <italic>roc</italic>
                <italic>arpum</italic>
              </td>
              <td>1</td>
            </tr>
            <tr>
              <td>7.</td>
              <td>
                <italic>Palm</italic>
                sp.
              </td>
              <td>1</td>
            </tr>
            <tr>
              <td colspan="2">Total ± SD</td>
              <td>829 ±267.99</td>
              <td colspan="2">Total</td>
              <td>225 ±33.05</td>
              <td colspan="2">Total</td>
              <td>298 ±8.48</td>
              <td colspan="2">Total</td>
              <td>155 ±28.13</td>
            </tr>
            <tr>
              <td colspan="2">CV</td>
              <td>64.65</td>
              <td colspan="2">-</td>
              <td>102.83</td>
              <td colspan="2">-</td>
              <td>5.69</td>
              <td colspan="2">-</td>
              <td>90.74</td>
            </tr>
          </tbody>
        </table>
      </table-wrap>
      <p>Note: Each value is a total of 12 observations. CV: Coefficient variability.</p>
      <fig id="fig1">
        <label>Figure 1</label>
        <graphic xlink:href="https://html.scirp.org/file/8303854-rId11.jpeg?20260604100838" />
      </fig>
      <p><bold>Figure 1</bold><bold>.</bold>The per cent occurrence of nest host trees offered by <italic>Apis dorsata</italic>during summer season.</p>
      <fig id="fig2">
        <label>Figure 2</label>
        <graphic xlink:href="https://html.scirp.org/file/8303854-rId12.jpeg?20260604100838" />
      </fig>
      <p><bold>Figure 2</bold><bold>.</bold>The per cent occurrence of foraging plants to <italic>A. dorsata</italic> during summer season.</p>
      <p>summer in Mysore. Comparatively, tree species contributed more foraging source to <italic>A.</italic><italic>dorsata</italic> than that of shrubs and herbs. Altogether, 236 <italic>A.</italic><italic>dorsata</italic> colonies had good floral source and water source was good at 155 <italic>A.</italic><italic>dorsata</italic> colonies during summer season (<bold>Table 4</bold>). However, for remaining 531 <italic>A.</italic><italic>dorsata</italic> colonies suffered due to poor availability of floral sources at their nesting place. <xref ref-type="fig" rid="fig3">Figure 3</xref> shows the foraging source extended to <italic>A.</italic><italic>dorsata</italic> by different plant families during summer season in Mysore. Total 27 plant families extended their floral source (pollen and nectar) to <italic>A.</italic><italic>dorsata</italic> during summer months <italic>i.e.</italic>, March to June. Of all, Fabaceae family members were predominantly supplied floral source (both nectar and pollen) and it was followed by Apocyanaceae, Asteraceae, Moraceae and Acanthaceae family members. Remaining 18 plant families supplied considerable amount of nectar and pollen source to <italic>A.</italic><italic>dorsata</italic> during summer season in Mysore (<xref ref-type="fig" rid="fig3">Figure 3</xref>). Furthermore, <italic>A.</italic><italic>dorsata</italic> depended for its water requirement from tap water, water leaked from the water supplying pipes, stagnant water, water tanks, ponds/lakes and sewage canals and exhibited considerable coefficient variation (CV = 90.74) during summer season in Mysore (<bold>Table 4</bold>).</p>
      <fig id="fig3">
        <label>Figure 3</label>
        <graphic xlink:href="https://html.scirp.org/file/8303854-rId13.jpeg?20260604100838" />
      </fig>
      <p><bold>Figure 3</bold><bold>.</bold> The plant families extended the floral source to <italic>Apis dorsata</italic> population during summer season.</p>
      <p><bold>Anthr</bold><bold>opogenic</bold><bold>interferences:</bold><italic>A.</italic><italic>dorsata</italic> experienced several biological constraints during summer season under natural conditions of Mysore. Being an open nester, it is commonly experiencing various interferences at its nesting sites. Amongst the biological constraints, man-made activities intervene with the life of <italic>A.</italic><italic>dorsata</italic> midst urban conditions during summer season. The level of human interference was low with 37 <italic>A.</italic><italic>dorsata</italic> colonies and it was followed medium level with 50 <italic>A.</italic><italic>dorsata</italic> colonies and high level with 77 colonies with a coefficient variation 37.32 (<bold>Table 5</bold>). Further, killing of <italic>A.</italic><italic>dorsata</italic> population, burning of live and deserted colonies, spraying of pesticides on the colonies and hunting of <italic>A.</italic><italic>dorsata</italic> colonies were observed at Mysore city during summer season (<bold>Table 5</bold>). Despite various interferences, <italic>A.</italic><italic>dorsta</italic> showed nest site fidelity to its previously nested site in Mysore. Total 84 <italic>A.</italic><italic>dorsata</italic> colonies did show site fidelity among 829 <italic>A.</italic><italic>dorsata</italic> colonies and that represented 10.1% nest site fidelity to its previously nested site with a coefficient variation 26.90 (<bold>Table 5</bold>). The fidelity status information was collected by personal interaction with the local residents along with regular observation of nest marks (chemical signature – wax marks of nests) (<bold>Table 5</bold>).</p>
      <p><bold>Table 5.</bold><bold>Commonly occurring human interferences at the vicinity of</bold><bold>giant honeybee,</bold><italic><bold>Apis dorsata</bold></italic><bold>colonies recorded during summer season in Mysore</bold><bold>.</bold></p>
      <table-wrap id="tbl5">
        <label>Table 5</label>
        <table>
          <tbody>
            <tr>
              <td>Sl.No.</td>
              <td>Level of human interference</td>
              <td>No. of colonies</td>
              <td>Sl.No.</td>
              <td>Nature of interference</td>
              <td>No. of incidences</td>
              <td>Sl.No.</td>
              <td>Nest site Fidelity</td>
              <td>No. of colonies</td>
            </tr>
            <tr>
              <td>1.</td>
              <td>Low</td>
              <td>37</td>
              <td>1.</td>
              <td>Killing</td>
              <td>17</td>
              <td>1.</td>
              <td>By interview</td>
              <td>34</td>
            </tr>
            <tr>
              <td>2.</td>
              <td>Medium</td>
              <td>50</td>
              <td>2.</td>
              <td>Burning</td>
              <td>7</td>
              <td rowspan="3">2.</td>
              <td rowspan="3">Nest marks</td>
              <td rowspan="3">22</td>
            </tr>
            <tr>
              <td rowspan="2">3.</td>
              <td rowspan="2">High</td>
              <td rowspan="2">77</td>
              <td>3.</td>
              <td>Pesticide spray</td>
              <td>12</td>
            </tr>
            <tr>
              <td>4.</td>
              <td>Hunting</td>
              <td>3</td>
            </tr>
            <tr>
              <td colspan="2">Total ± SD</td>
              <td>164.0 ± 20.40</td>
              <td colspan="2">Total</td>
              <td>39.0 ± 6.08</td>
              <td colspan="2">Total</td>
              <td>84.0 ± 11.30</td>
            </tr>
            <tr>
              <td colspan="2">CV</td>
              <td>37.32</td>
              <td colspan="2">-</td>
              <td>62.36</td>
              <td colspan="2">-</td>
              <td>26.90</td>
            </tr>
          </tbody>
        </table>
      </table-wrap>
      <p>Note: Each value is total of 12 observations. CV: Coefficient variability.</p>
    </sec>
    <sec id="sec4">
      <title>4. Discussion</title>
      <p>The giant honeybee, <italic>Apis</italic><italic>dorsata</italic> Fabricius (1793) (Hymenoptera: Apidae) [[<xref ref-type="bibr" rid="B46">46</xref>], [<xref ref-type="bibr" rid="B47">47</xref>]] is a non-domesticated species [[<xref ref-type="bibr" rid="B35">35</xref>], [<xref ref-type="bibr" rid="B36">36</xref>], [<xref ref-type="bibr" rid="B48">48</xref>]], live under wild conditions by establishing normally “U” shaped, big sized single comb [[<xref ref-type="bibr" rid="B5">5</xref>]] at higher elevations at the eave of multistoried man-made establishments and tall trees [[<xref ref-type="bibr" rid="B12">12</xref>], [<xref ref-type="bibr" rid="B13">13</xref>], [<xref ref-type="bibr" rid="B16">16</xref>], [<xref ref-type="bibr" rid="B18">18</xref>], [<xref ref-type="bibr" rid="B49">49</xref>]]. Being an open nester, it builds colonies based on locally available good source of nectar plants, pollen plants, water source and undisturbed conditions [[<xref ref-type="bibr" rid="B15">15</xref>], [<xref ref-type="bibr" rid="B16">16</xref>], [<xref ref-type="bibr" rid="B21">21</xref>], [<xref ref-type="bibr" rid="B41">41</xref>], [<xref ref-type="bibr" rid="B43">43</xref>], [<xref ref-type="bibr" rid="B44">44</xref>]], However, during the present investigation, 829 <italic>A. dorsata</italic> colonies were recorded during summer season in Mysore. <italic>A. dorsata</italic> preferred 164 nest sites to establish 604 colonies on man-made structures, which included Commercial buildings (45 sites), Educational buildings (27 sites), Residential buildings (22 sites), Government offices and Water Tanks (15 sites each), Hotels, Hospitals, Conventional Halls, Religious and Heritage buildings to establish colonies during summer season. Similar type of observations was made by [[<xref ref-type="bibr" rid="B10">10</xref>], [<xref ref-type="bibr" rid="B21">21</xref>]] in Mysore, [[<xref ref-type="bibr" rid="B19">19</xref>]] in Bangalore. Interestingly, during the present study, only on 15 sites, <italic>A. dorsata</italic> established 225 colonies on seven tree species namely: <italic>Ceiba petandra, Ficus benghalensis, Albizia saman, Ficus religosa, Pterogyta allata, Peltophorum pterocarpum</italic> and <italic>Palm</italic> species. Of all, <italic>C. petandra, F. benghalensis</italic> and <italic>A. saman</italic> were more preferred by <italic>A. dorsata</italic> during summer season in Mysore. Further, <italic>A.</italic><italic>dorsata</italic> preferred 10 to 110 feet elevation to establish colonies on these tree species. Perhaps, it is due to their well ramified canopy with broad surfaced tree limbs along with undisturbed navigation path during most of the day in summer season which might have encouraged <italic>A. dorsata</italic> to establish its nests more on the eave of these tree limbs [[<xref ref-type="bibr" rid="B12">12</xref>]]. Interestingly, 40 to 49 feet elevation was more preferred, where 279 <italic>A. dorsata</italic>colonies were observed. Medium height extended 51 nesting sites and hosted 1,434 nests in and around Bangalore [[<xref ref-type="bibr" rid="B27">27</xref>]]. However, 30 to 39 and 20 to 29 feet elevation was offered by <italic>A.</italic><italic>dorsata</italic> to establish 242 and 212 colonies respectively in Mysore. Moreover, <italic>A.</italic><italic>dorsata</italic> also nested at 10 to 19, 50 to 59, 60 to 69, 70 to 79, 80 to 89, 90 to 99 and 100 to 110 feet heights. The Mysore is located at an elevation 770 meters above sea level at the vicinity of Chamundi Hills and it is housed with many natural ponds/lakes [[<xref ref-type="bibr" rid="B40">40</xref>]]. Water is available at parks, neatly maintained lawns, sewage canals and other sources, which could help provide required water source to <italic>A. dorsata</italic> colony members during summer season. Moreover, the city possesses a salubrious climate with temperature ranging from 24˚C to 35˚C during summer season [[<xref ref-type="bibr" rid="B40">40</xref>]] and locally prevailed scrubby type vegetation along with tropical deciduous trees which bear abundant flowers during March to end of May months. Further, Mysore is one of the big cities in Karnataka, popularly called “City of Palaces” [[<xref ref-type="bibr" rid="B40">40</xref>]], known for tall buildings, big sized trees with ramified broad limbed long branches. The man-made structures, tall religious /heritage buildings are provided suitable nest sites to <italic>A. dorsata</italic> population [[<xref ref-type="bibr" rid="B5">5</xref>], [<xref ref-type="bibr" rid="B12">12</xref>]]. Surprisingly, these man-made structures and few tall trees are monitored and maintained under heritage tag by the local administration in Mysore [[<xref ref-type="bibr" rid="B40">40</xref>]]. As a result, no disturbance was made with many tall trees and their broad limbed branches, which are untrimmed and several heritage/religious buildings/monuments, office buildings are undisturbed. Perhaps, this could be one of the reasons for recording more <italic>A. dorsata</italic> colonies on man-made structures in Mysore during summer. Moreover, several multistoried human inhabited buildings are not accessed easily to mankind. And, these man-made tall structures and few tree species might have supported the <italic>A. dorsata</italic> colonies from the direct sunlight, wind and rainfall [[<xref ref-type="bibr" rid="B19">19</xref>]] and perhaps, reduced human interference [[<xref ref-type="bibr" rid="B50">50</xref>]]. Perhaps, all these locally prevailed conditions might have extended the increased protection to the normal survival of <italic>A. dorsata</italic> during summer season in Mysore. Similar type of observations was made by [[<xref ref-type="bibr" rid="B5">5</xref>], [<xref ref-type="bibr" rid="B8">8</xref>], [<xref ref-type="bibr" rid="B9">9</xref>], [<xref ref-type="bibr" rid="B12">12</xref>], [<xref ref-type="bibr" rid="B13">13</xref>], [<xref ref-type="bibr" rid="B16">16</xref>], [<xref ref-type="bibr" rid="B18">18</xref>], [<xref ref-type="bibr" rid="B19">19</xref>], [<xref ref-type="bibr" rid="B21">21</xref>], [<xref ref-type="bibr" rid="B34">34</xref>], [<xref ref-type="bibr" rid="B43">43</xref>]] at Bangalore district in Karnataka, in China, in Niligiri Biosphere Reserve, in southern Karnataka, in Mysore, district and in Subah, Borneo, Indonesia, in Nepal, India and Bhutan. Thus, present reports are on par with the earlier published reports.</p>
      <p>Despite, locally prevailed undisturbed conditions at many man-made structures, <italic>A. dorsata</italic>colony population showed fluctuations in its colony size. Since, <italic>A. dorsata</italic> is a migratory species [[<xref ref-type="bibr" rid="B29">29</xref>], [<xref ref-type="bibr" rid="B30">30</xref>], [<xref ref-type="bibr" rid="B51">51</xref>], [<xref ref-type="bibr" rid="B52">52</xref>]] which establish colonies seasonally [[<xref ref-type="bibr" rid="B29">29</xref>]] and migrate from one place to other to complete swarming cycle and in search of good floral source, suitable nesting conditions in the wild [[<xref ref-type="bibr" rid="B5">5</xref>], [<xref ref-type="bibr" rid="B8">8</xref>], [<xref ref-type="bibr" rid="B17">17</xref>], [<xref ref-type="bibr" rid="B44">44</xref>]]. Moreover, locally occurring biological constraints [[<xref ref-type="bibr" rid="B15">15</xref>]] during different seasons interfere with their normal activity and affect the colony survival of <italic>A. dorsata</italic>. So, overall, 485 live colonies and 344 deserted colonies of <italic>A. dorsata</italic> were recorded during summer season in Mysore. So, desertification of a colony occurs due to various man-made interferences or by natural means or due to swarming cycle [[<xref ref-type="bibr" rid="B29">29</xref>], [<xref ref-type="bibr" rid="B30">30</xref>], [<xref ref-type="bibr" rid="B51">51</xref>], [<xref ref-type="bibr" rid="B52">52</xref>]]. Thus, the colony size varied considerably and they were grouped into small (180), medium (418) and big sized (231) colonies. The colonies were “U”, “V”, “irregular” and “dome” shape in their appearance with different orientations. Interestingly, “U” shaped colonies were more predominant (673) compared to others. Thus, <italic>A. dorsata</italic> established different sized, shaped colonies with north-south, east-west, northeast-southwest and northwest-south easterly orientations on man-made structures and on trees during summer season. Of all, north-southerly oriented colonies [[<xref ref-type="bibr" rid="B53">53</xref>]] were more (295) compared to other orientations during summer season. Being an open nester, perhaps to avail sunlight and to have good navigation path, <italic>A. dorsata</italic> established differently oriented colonies with varied size and shape at various man-made structures and on trees during summer season. Thus, establishing comb with different orientations helped to achieve safety and protection from direct sunlight, wind [[<xref ref-type="bibr" rid="B23">23</xref>]], rainfall and to avail good navigation path [[<xref ref-type="bibr" rid="B54">54</xref>]] for easy movement to conduct hive-out activities by the worker bees [[<xref ref-type="bibr" rid="B19">19</xref>], [<xref ref-type="bibr" rid="B34">34</xref>]]. Although, locally prevailed conditions are suitable, <italic>A. dorsata</italic> obviously constructed uniform sized colonies, but the size and shape of many colonies were uneven, perhaps it was influenced by the locally available varied conditions at man-made establishments and on trees. Hence, to avail the good navigation path and to avoid direct sunlight, wind and rainfall on the comb [[<xref ref-type="bibr" rid="B34">34</xref>]], <italic>A.</italic><italic>dorsata</italic> established different shaped combs under arboreal conditions. Or, pests or predatory animal’s regular interferences [[<xref ref-type="bibr" rid="B5">5</xref>], [<xref ref-type="bibr" rid="B15">15</xref>]] might have discouraged to attain big size of the colonies. Or, <italic>A.</italic><italic>dorsata</italic> perhaps might have involved in defencing the colony due to various biological constraints (e.g. Wax moth infestation or predators interferences) [[<xref ref-type="bibr" rid="B15">15</xref>]] rather to expand the colony vertically or horizontally to build big sized comb with specific orientation. Or, perhaps small to medium sized colonies established recently and they were younger and not older.</p>
      <p>Floral source was good at the vicinity of 143 <italic>A.</italic><italic>dorsata</italic> colonies during summer season in Mysore. The vegetation consists of more tree species than that of shrubs and herbs which belong to 27 families [[<xref ref-type="bibr" rid="B55">55</xref>]] extended floral source to <italic>A.</italic><italic>dorsata</italic> colonies. Of all, many Fabaceae family members were more predominant during summer due to high temperature [[<xref ref-type="bibr" rid="B56">56</xref>]] that could help bloom the flowers continuously to supply floral source (both nectar and pollen) to <italic>A.</italic><italic>dorsata</italic> population [[<xref ref-type="bibr" rid="B44">44</xref>]]. The Apocyanaceae, Asteraceae, Moraceae and Acanthaceae family members also extended good source of both nectar and pollen to <italic>A.</italic><italic>dorsata</italic> population. Perhaps, this might be one of the reasons for big sized colonies due to increased foraging and brood rearing activity [[<xref ref-type="bibr" rid="B17">17</xref>]] at some places in Mysore city. The water source was good at the vicinity of 155 <italic>A. dorsata</italic> colonies and it was gathered from tap water, water from the water supplying pipes leakage, stagnant water, water tanks, ponds/lakes and sewage canals during summer season in Mysore city. Since summer is characterized by high (&lt;36˚C) temperature [[<xref ref-type="bibr" rid="B40">40</xref>]], being a poikilothermic organism [[<xref ref-type="bibr" rid="B46">46</xref>]], <italic>A.</italic><italic>dorsata</italic> require more water to cool its brood and maintain comb temperature below 36˚C. Perhaps, to protect the colony and brood from high temperature, majority of the worker bees might have engaged with water collection from faraway places rather than pollen and nectar collection to increase breeding activity and expanding the colony. Perhaps, different sized colonies occurred due to all these reasons during the present study. <italic>A.</italic><italic>dorsata</italic> preferred 13 different coloured surfaces on various man-made structures to establish its colonies during summer season. Similar type of observations was made by [[<xref ref-type="bibr" rid="B10">10</xref>], [<xref ref-type="bibr" rid="B26">26</xref>], [<xref ref-type="bibr" rid="B57">57</xref>]-[<xref ref-type="bibr" rid="B59">59</xref>]] on man-made structures at different parts of the world including India. Surprisingly, white surfaced objects were the most commonly preferred by <italic>A. dorsata</italic>and accordingly more (205) colonies were established on white surfaced objects during summer season. However, brown, yellow and grey colour surfaced objects were also preferred by <italic>A. dorsata</italic> for colony establishment during summer season. <italic>A.</italic><italic>dorsata</italic> prefer different coloured surfaces to establish its colony on man-made structures [[<xref ref-type="bibr" rid="B10">10</xref>]]. Excepting red, perhaps, many colours including white colour might help retrieve the visuo-spatial memories [[<xref ref-type="bibr" rid="B60">60</xref>]] using visual navigation [[<xref ref-type="bibr" rid="B54">54</xref>]] while establishing the new colony on previously nested sites [[<xref ref-type="bibr" rid="B10">10</xref>]]. White surfaced objects of various man-made structures have attracted more by the new <italic>A.</italic><italic>dorsata</italic> swarm [[<xref ref-type="bibr" rid="B10">10</xref>]] and perhaps that might have provided a visual cue, help recollect the memory of previously nested site [[<xref ref-type="bibr" rid="B61">61</xref>]] by worker bees [[<xref ref-type="bibr" rid="B42">42</xref>]] to decide [[<xref ref-type="bibr" rid="B41">41</xref>]] site for colony establishment. Interestingly, glass surface was also chosen by <italic>A.</italic><italic>dorsata</italic> for establishing its colonies during summer season at Mysore. Despite various interferences, <italic>A.</italic><italic>dorsta</italic> showed nest site fidelity to its previously nested site [[<xref ref-type="bibr" rid="B43">43</xref>]]. Total 84 <italic>A.</italic><italic>dorsata</italic> colonies did show site fidelity among 829 <italic>A.</italic><italic>dorsata</italic> colonies and that represented 10.1% nest site fidelity to its previously nested sites [[<xref ref-type="bibr" rid="B52">52</xref>]]. Further, <italic>A</italic>. <italic>dorsata</italic> leave chemical signature (wax marks) while departing the colony during swarming or desertification [[<xref ref-type="bibr" rid="B43">43</xref>]]. These wax marks are used as landmarks for establishing colonies at previously nested sites [[<xref ref-type="bibr" rid="B10">10</xref>], [<xref ref-type="bibr" rid="B30">30</xref>]] and thus <italic>A.</italic><italic>dorsata</italic> demonstrated the fidelity to its previous nest sites [[<xref ref-type="bibr" rid="B52">52</xref>]], this behavior varied considerably between different colour surfaced objects [[<xref ref-type="bibr" rid="B10">10</xref>]] and thus <italic>A.</italic><italic>dorsata</italic> return to its previous nest sites [[<xref ref-type="bibr" rid="B30">30</xref>]]. Thus, <italic>A.</italic><italic>dorsata</italic> exhibited elite nesting strategy [[<xref ref-type="bibr" rid="B5">5</xref>], [<xref ref-type="bibr" rid="B28">28</xref>], [<xref ref-type="bibr" rid="B62">62</xref>]-[<xref ref-type="bibr" rid="B67">67</xref>]] and revealed site fidelity. Recording nest site fidelity status is very essential to protect previously nested sites to allow new swarm to establish colony [[<xref ref-type="bibr" rid="B30">30</xref>], [<xref ref-type="bibr" rid="B43">43</xref>]], thereby it is possible to restore <italic>A.</italic><italic>dorsata</italic> population amidst their preferred habitats. Despite all the above mentioned prevailed conditions during summer season under urban ecosystems, <italic>A.</italic><italic>dorsata</italic>, being an open nester [[<xref ref-type="bibr" rid="B8">8</xref>]], experienced several biological constraints [[<xref ref-type="bibr" rid="B15">15</xref>], [<xref ref-type="bibr" rid="B16">16</xref>], [<xref ref-type="bibr" rid="B35">35</xref>], [<xref ref-type="bibr" rid="B36">36</xref>]] amidst different nest sites in Mysore. The level of human interference was low with 37 <italic>A.</italic><italic>dorsata</italic> colonies, medium level of interferences with 50 <italic>A.</italic><italic>dorsata</italic> colonies and high level of interferences with 77 <italic>A.</italic><italic>dorsata</italic> colonies. <italic>A.</italic><italic>dorsata</italic> population was killed from live colonies by hunting, burning and spraying pesticides at different nest sites during summer season. All these man-made interferences perhaps discouraged the development of colony growth [[<xref ref-type="bibr" rid="B15">15</xref>]] and resulted to occur different sized colonies during summer season at different nest sites in Mysore. This kind of man-made activities are more in recent past, as a result honeybee colony population is seriously threatened by deforestation, agricultural intensification, monoculture farming, extensive pesticide use, destructive honey hunting activities [[<xref ref-type="bibr" rid="B10">10</xref>], [<xref ref-type="bibr" rid="B68">68</xref>]], forest fires, prolonged droughts, increasing temperature and regional/local climate change [[<xref ref-type="bibr" rid="B35">35</xref>], [<xref ref-type="bibr" rid="B36">36</xref>]] at different parts of the world. Hence, honeybee population is facing unprecedented threats due to various reasons [[<xref ref-type="bibr" rid="B15">15</xref>]]. Perhaps, this might be one of the reasons for <italic>A.</italic><italic>dorsata</italic> colonies population to fluctuate during different seasons [[<xref ref-type="bibr" rid="B16">16</xref>]]. Since, <italic>A.</italic><italic>dorsata</italic> represents one of the biologically diverse and ecologically indispensable species, its survival is partly/fully linked to global food security, local/regional biodiversity and ecosystem resilience. Astonishingly, <italic>A.</italic><italic>dorsata</italic> is one of the potential pollinators [[<xref ref-type="bibr" rid="B3">3</xref>]], recording its colony population is need of the hour during different seasons to monitor local/regional biodiversity [[<xref ref-type="bibr" rid="B2">2</xref>], [<xref ref-type="bibr" rid="B5">5</xref>], [<xref ref-type="bibr" rid="B67">67</xref>]]. Surprisingly, human association with honeybee’s existed dates back <italic>i.e.</italic>, around thousands of years ago with evidence of beekeeping in ancient Egyptian, Greek and Mesoamerican civilizations [[<xref ref-type="bibr" rid="B1">1</xref>]]. This long-standing relationship underscores the importance of honeybees not only as economic resources but, it could help in poverty alleviation of many people across the world [[<xref ref-type="bibr" rid="B2">2</xref>], [<xref ref-type="bibr" rid="B69">69</xref>]]. Therefore, this kind of season specific investigations are essential for developing effective conservation-cum-management strategies [[<xref ref-type="bibr" rid="B5">5</xref>], [<xref ref-type="bibr" rid="B38">38</xref>], [<xref ref-type="bibr" rid="B70">70</xref>]] and to reap the bee products [[<xref ref-type="bibr" rid="B69">69</xref>]] and pollination role for poverty alleviation as well. Hence, present study help reveal status [[<xref ref-type="bibr" rid="B71">71</xref>], [<xref ref-type="bibr" rid="B72">72</xref>]] of wild honeybee, <italic>A.</italic><italic>dorsata</italic> population and justified the need to understand the prevailed conditions to protect locally existed pollinators population [[<xref ref-type="bibr" rid="B38">38</xref>], [<xref ref-type="bibr" rid="B70">70</xref>]] during summer season amidst urban environmental conditions.</p>
    </sec>
    <sec id="sec5">
      <title>AcknowledgementS</title>
      <p>Authors thankful to the local people of Mysore for their inputs during field investigations. Authors also thankful to the Chairman, DOS in Zoology, University of Mysore, Mysore for the facility.</p>
    </sec>
  </body>
  <back>
    <ref-list>
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