The study adopted a descriptive survey research design, which involves collecting, organizing, analyzing, and interpreting data to describe the characteristics, opinions, behaviors, or attitudes of a population or phenomenon without manipulating variables. The design was chosen because it focuses on exploring participants’ views using open-ended instruments such as interviews or narrative responses. In this sense, the design remains descriptive by portraying phenomena as they naturally occur, while relying on qualitative methods to generate rich, in-depth data.

The research was carried out in six Local Government Areas (LGAs) spanning the three senatorial zones of Anambra State, Nigeria. The target population comprised 17,372 individuals, including caregivers, parents, and healthcare workers. A sample of 360 respondents was determined using the Krejcie and Morgan Table for sample size calculation. A sample size of 360 was considered appropriate because, according to the Krejcie and Morgan Table for sample size determination, it provides a statistically representative subset of a population of 17,372, ensuring adequate generalization of findings with acceptable precision and confidence.

This study was conducted using a multistage sampling technique. Initially, the state was divided into three senatorial zones—Anambra Central, Anambra North, and Anambra South—each consisting of seven LGAs. Subsequently, two LGAs were randomly selected from each zone for inclusion in the study. A multistage sampling technique was employed to enhance representativeness and manageability by selecting samples in successive stages, thereby ensuring adequate coverage of the study population while reducing cost and logistical complexity.

With a total sample size of 360, the participants were distributed as follows: 150 adolescents, 90 parents, 60 community leaders, 30 routine immunization service providers (health facility level), 24 health care providers (local government level) and 6 state-level immunization program officers to ensure adequate representation of the key stakeholder groups. The qualitative component comprised 12 Focus Group Discussions (FGDs) with adolescents, parents, health facility routine immunization service providers and community leaders while 20 Key Informant Interviews (KIIs) were conducted with state immunization program officers, local government immunization program officers and selected community representatives. This combination ensured both breadth and depth of data, with FGDs capturing shared experiences and KIIs providing expert and contextual insights.

For data collection, a structured interview guide was developed and used in the selected communities. Focus Group Discussions (FGDs) were conducted with members of the community including community leaders, parents, adolescents and healthcare providers. Key Informant Interviews (KIIs) were conducted with the state and LGA level immunization officers, health promoters and health care providers. The qualitative data generated alongside the survey were analyzed using a thematic analysis procedure following a systematic, multi-step process to ensure rigor and reproducibility. First, all interview and FGD recordings were transcribed verbatim and cross-checked against audio files for accuracy by two trained research assistants. Familiarization was achieved through repeated reading of the transcripts, after which initial open coding was conducted independently by two coders (the principal researcher and one assistant), who manually identified meaningful units of text related to knowledge, awareness, misconceptions, and information sources on HPV and the HPV vaccine. A preliminary codebook was then developed, and codes were compared, discussed, and refined through consensus meetings, where overlapping codes were merged and ambiguous ones clarified. Next, axial coding was applied to group related codes into broader categories (e.g., “awareness of HPV,” “misconceptions,” “sources of information,” and “knowledge gaps”), which were subsequently synthesized into overarching themes reflecting patterns in participants’ responses. Inter-coder discrepancies were resolved through discussion and, where necessary, consultation with a third reviewer to ensure consistency and credibility of the analysis. The final themes were reviewed against the raw data to confirm that they accurately represented participants’ views, and illustrative excerpts were selected to support each theme. The analysis was conducted manually without the use of specialized qualitative data analysis software, although Microsoft Word and Excel were used to organize transcripts, codes, and thematic matrices.

Parents exhibited moderate awareness of HPV, commonly associating it with cervical cancer but demonstrating limited understanding of the vaccine’s purpose. One parent stated, “I know it [HPV] causes cervical cancer, but I am not sure how the vaccine works.” This indicates a partial awareness of HPV-related health risks but a gap in understanding the preventive potential of vaccination. Adolescents showed mixed knowledge: while some could identify HPV, many were unclear about transmission or vaccine function. A female adolescent noted, “I heard about HPV in school, but I thought it only affects girls when they are older.” In contrast, healthcare workers exhibited high knowledge, accurately describing HPV types, transmission, and vaccination schedules. A nurse observed, “HPV is a virus that can cause cervical and other cancers, but vaccination can prevent infection if given before sexual debut.” These findings suggest that professional training enhances awareness, while public and adolescent knowledge remains limited, highlighting the need for targeted education programs.

Table 1. Schematic representation of perception of HPV and HPV vaccine in Anambra state.

Attitudes toward HPV vaccination varied significantly among groups. Parents demonstrated a dual attitude, showing support when they recognized preventive benefits but also hesitancy due to concerns about safety and cultural or religious beliefs. One parent said, “If this vaccine can protect my daughter from cancer, I will make sure she gets it,” while another expressed caution: “I am not sure about this vaccine; I don’t want my child to have side effects.” Adolescents were generally willing to vaccinate, but their decision was influenced by parents and healthcare worker advice. One adolescent noted, “If my parents or doctors say it is safe, I will take it, but I was worried at first.” Healthcare workers, however, were strongly supportive and proactive in promoting vaccination. A healthcare worker emphasized, “We always encourage parents to vaccinate their children early. Prevention is better than cure.” The findings highlight that attitudes are shaped by both knowledge and perceived benefits, while hesitancy is largely driven by safety concerns and cultural factors.

Misconceptions and cultural beliefs influenced vaccine perception. Some parents feared that vaccination might encourage early sexual activity, as reflected in the comment, “If I give my child this vaccine, does it mean they can start having sex? That worries me.” Adolescents occasionally confused vaccination with treatment for existing infections. Healthcare workers were aware of these misconceptions and advocated for culturally sensitive education: “Many parents are hesitant because of traditional beliefs. We try to explain that vaccination is about health, not morality.” This highlights that while knowledge is necessary, addressing cultural beliefs and misinformation is critical for improving vaccine acceptance.

Trust emerged as a key determinant of perception. Parents and adolescents relied heavily on healthcare workers for accurate information, often giving less credence to social media or community hearsay. Most of their reasons for rejection are as a result of misinformation from social media, some religious leaders and so on. As a healthcare worker observed, “Parents listen more when the information comes from health workers rather than the internet or hearsay.” The findings underscore the central role of healthcare providers in influencing perceptions and promoting vaccine uptake, particularly through clear and credible communication.

Overall, perception of HPV and its vaccine reflects a gradient of awareness and acceptance. Healthcare workers demonstrated high knowledge and strong advocacy, adolescents were moderately receptive but dependent on parental and professional guidance, and parents showed cautious acceptance influenced by partial knowledge, cultural beliefs, and trust. These results indicate that improving perception and vaccine uptake in Anambra State requires targeted educational interventions, culturally sensitive communication, and active engagement of healthcare professionals as trusted sources of information.

The availability of HPV vaccines in health facilities in Anambra State presented on Table 2 was explored through in-depth interviews with healthcare workers and facility managers. Analysis revealed several critical themes influencing vaccine availability, including stock management, cold chain capacity, health worker training, accessibility, and policy support.

Table 2. Schematic representation of availability of the HPV vaccines in health facilities in Anambra state.

Stock management emerged as a major challenge across facilities. Healthcare workers reported intermittent stock levels and frequent stock-outs, affecting the regular administration of HPV vaccines. One participant explained, “Sometimes we run out of the HPV vaccine for weeks because the next delivery has not arrived.” Delays in replenishment from government or partner agencies were common: “We depend on state supply, but deliveries are irregular.” Poor forecasting and ordering practices were also noted, with many facilities relying on manual stock registers that were often outdated. These findings suggest that inconsistent supply chains and inadequate inventory management significantly hinder vaccine availability.

Cold chain limitations were identified as a barrier to vaccine availability. A significant proportion of health facilities rely on Solar Direct Drive (SDD) freezers as their sole source of cold chain infrastructure for the storage of vaccines and other temperature-sensitive medical commodities. These SDD freezers are designed to operate independently of grid electricity by harnessing solar energy, making them particularly suitable for rural and hard-to-reach settings where power supply is unreliable or entirely absent. As a result, they play a critical role in sustaining immunization services and safeguarding the potency of vaccines at the point of service delivery.

However, despite their strategic importance, a notable number of these SDD freezers are currently non-functional or performing below optimal standards. The causes of non-functionality are often multifaceted, including inadequate routine maintenance, delayed repairs, lack of trained technicians, aging equipment, damaged solar panels, battery system failures, and exposure to harsh environmental conditions. In some facilities, the absence of alternative cold chain equipment or backup power sources means that when an SDD freezer breaks down, there is no immediate substitute to ensure uninterrupted cold storage.

Limited staff training in vaccine logistics further affected availability. While healthcare workers were generally capable of administering the vaccine, many lacked specific training in stock management and forecasting. As one staff member noted, “Not all staff know how to handle HPV vaccine requirements or forecasting.” Facilities often relied on personnel who could deliver vaccines but struggled to maintain adequate inventory levels. These results highlight the importance of capacity building for staff in both administration and logistics management.

Accessibility of HPV vaccines varied across urban and rural facilities. Urban health centers generally reported more consistent supply, whereas rural facilities experienced prolonged stock gaps. Outreach and community clinics often reported lower availability, with HPV sometimes excluded from immunization campaigns due to limited supply. These disparities indicate that geographical location significantly affects vaccine access and highlight the need for equitable distribution strategies.

Policy and coordination challenges were also evident. Healthcare workers reported weak communication between facilities and supply chain managers, which hindered timely planning: “We often don’t get advance notice of delivery schedules.” Additionally, HPV vaccines were perceived as low priority compared to childhood vaccines within routine immunization programs. Some staff noted that this lower prioritization contributed to stock shortages and inconsistent availability. Strengthening policy support and supply chain coordination is therefore critical to improving HPV vaccine availability.

Overall, the availability of HPV vaccines in Anambra State is constrained by multiple systemic factors. Inconsistent supply, cold chain limitations, uneven distribution, limited staff training, and low prioritization collectively reduce the reliability of vaccine provision. Urban facilities fare better than rural ones, highlighting geographic disparities. These findings suggest that improving HPV vaccine availability requires enhanced supply chain management, infrastructure investment, staff training, and prioritization of HPV vaccines within routine immunization policies.

The result presented on Table 3 explored the acceptance of the HPV vaccine among parents, adolescents, and healthcare workers in Anambra State. Data from focus group discussions with parents and adolescents, and in-depth interviews with healthcare workers, revealed patterns of willingness, barriers, influencing factors, and overall attitudes toward vaccination.

Willingness to vaccinate varied across the study population. Among parents, willingness was high when informed, as reflected in a parent’s statement: “If the vaccine protects my daughter from cancer, I will make sure she receives it.” Adolescents demonstrated moderate willingness, largely influenced by parental guidance and healthcare advice: “I would take it if my parents approve and the doctor says it is safe.” Healthcare workers exhibited very high acceptance, consistently promoting vaccination and emphasizing its preventive benefits: “We always encourage HPV vaccination; it is an essential preventive measure.” These findings indicate that willingness is closely linked to knowledge and confidence in the vaccine’s safety and efficacy.

Table 3. Schematic representation of acceptance of HPV vaccine among the study population.

Despite overall positive attitudes, several barriers were identified. Parents and adolescents expressed safety concerns and fear of side effects, such as: “I am worried the vaccine might harm my child or make her sick.” Cultural and religious objections also influenced parental decisions, with some believing vaccination is unnecessary before marriage: “Some people believe vaccines for girls are unnecessary before marriage.” Additionally, misconceptions linking the vaccine to early sexual activity persisted: “I heard giving this vaccine encourages early sexual behavior, so I am not sure.” These barriers highlight that vaccine hesitancy is influenced not only by safety concerns but also by cultural norms and misinformation.

Several factors positively influenced vaccine acceptance. Trust in healthcare workers emerged as a key determinant, with participants more willing to vaccinate when benefits and safety were clearly explained. Adolescents were influenced by peer and community opinions, while prior exposure to education campaigns through schools, media, or health programs increased receptivity. These findings emphasize that trusted information sources and prior awareness are crucial for improving acceptance.

The overall attitude toward HPV vaccination varied by group. Parents demonstrated moderate to high acceptance, conditional on assurances regarding safety and professional advice. Adolescents exhibited moderate acceptance, largely dependent on parental guidance and credible health information. Healthcare workers consistently showed very high acceptance, actively advocating for vaccination and encouraging community uptake. These patterns suggest that targeted communication and engagement strategies are necessary to address hesitancy and enhance vaccine coverage.