jbm Journal of Biosciences and Medicines 2327-509X 2327-5081 Scientific Research Publishing 10.4236/jbm.2025.1312012 jbm-147860 Article Biomedical Life Sciences Involvement of Polymorphisms of the Interleukin 17A (rs2275913) and 17F (rs763780) Genes in the Development of Cervical Cancer and HPV Infection in Two West African Countries 0000-0001-5464-4571 Traoré Mah Alima Esther 1 2 Bambara Asseitou 1 0000-0001-5527-4648 Douamba Zoénabo 1 2 Zabré Pierre 1 Sétor Marius Ayaovi 1 0000-0002-9100-0692 Zohoncon Théodora Mahoukèdè 1 3 0000-0002-8814-4433 Sorgho Abel Pegdwendé 3 4 0000-0002-3121-8761 Ouédraogo Teega-Wendé Clarisse 1 0000-0002-4317-5930 Bado Prosper 1 0000-0002-9767-0646 Ouattara Abdoul Karim 1 0000-0003-0471-5057 Yonli Albert Théophane 1 4 0000-0002-6172-7487 Karou Simplice Damintoti 5 0000-0001-6957-3307 Ouédraogo Charlemagne 1 0000-0002-6895-6725 Djigma Florencia Wendkuuni 1 4 0000-0002-0415-9161 Simporé Jacques 1 4 Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, Ouagadougou, Burkina Faso National Center for Scientific and Technological Research (CNRST), Ouagadougou, Burkina Faso Faculty of Medicine, University Saint Thomas d’Aquin, Ouagadougou, Burkina Faso Pietro Annigoni Biomolecular Research Centre (CERBA), Ouagadougou, Burkina Faso Regional Center of Excellence in Poultry Science, University of Lomé, Lomé, Togo

The authors declare that there is no conflict of interest regarding the publication of this article.

 02 12 2025 12 2025 13 12 150 165 02 12 2025 © 2025 by the authors and Scientific Research Publishing Inc. 2025 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/ ). https://doi.org/10.4236/jbm.2025.1312012

Background: Interleukin-17 (IL-17) is a pro-inflammatory cytokine associated with inflammation, autoimmune disorders, and even tumors. Several studies have revealed abnormally high expression of IL-17 in human malignant tumors. The main objective of this study was to analyze the association between Single Nucleotide Polymorphisms (SNPs) of IL-17A (rs 2275913) and IL-17F (rs 763780) and their involvement in the development of cervical cancer in Burkina Faso and Togo, as well as their association with HPV. Methodology: In the present study, two SNPs in IL-17A (rs 2275913) and IL-17F (rs 763780) were analyzed in 44 patients with Cervical Cancer (CC) and 93 women with no history of cervical cancer from the city of Ouagadougou and 114 women with no history of cervical cancer from the city of Kara. Nucleic acids were extracted using the R-Biopharm kit. Polymorphism genotyping was performed by real-time PCR using TaqMan® tests. Results: Our results indicated that the G and A alleles were not associated with the different characteristics studied in the two cities. The frequency of genotypes of the rs2275913 polymorphism of the 1L-17A gene was 91.4%, 7.5%, and 1.1% for the GG, GA, and AA genotypes in Ouagadougou, 91.23%, 7.90%, and 3.23% for the TT, TC, and CC genotypes in Ouagadougou, and 89.50%, 10.50% and 0.00% respectively in Lomé in the city of Kara. The mutation rates of rs 2275913 and rs 763780 polymorphisms were 4.84% and 12.90% respectively for Ouagadougou, compared to 4.83% and 5.26% for Kara. There was no statistically significant difference between the genotypes of IL-17A (rs 2275913) and IL-17F (rs763780) polymorphisms in cervical cancer cases, and controls in Ouagadougou. Certain characteristics such as age at first sexual intercourse and parity were associated with IL-17A (rs 2275913) genotypes in Ouagadougou and age at first sexual intercourse in Kara. Conclusion: The results indicate that IL-17A and IL-17F polymorphisms are influenced by certain characteristics of women, but are not involved in the development of cervical cancer.

 Cervical Cancer HPV IL-17 Polymorphisms Burkina Faso Togo 1. Introduction

Cervical cancer is a common malignant tumor worldwide, with nearly 660,000 new cases and 350,000 deaths in 2022. Approximately 90% of cases occur in developing countries [1]. Cervical cancer, the second most common cancer among women worldwide, is one of the leading causes of cancer death [1], with Human Papillomavirus (HPV) infections identified as the main risk factor in its development [2]. The progression of HPV infection in the host can be multifactorial, including infection with the HPV type with the highest oncogenic risk [3]. Sociodemographic factors and the state of the host’s immune system, including genetic polymorphisms, play a role in the immune response [3][4]. Numerous studies have reported that polymorphisms in a series of genes, including those of interleukin-17 (IL-17), are associated with susceptibility to cervical cancer [5][6]. IL-17 is a pro-inflammatory cytokine secreted by various cells, including T helper cells, CD8+ T cells, CD4+ T cells, and natural killer cells in the tumor microenvironment [7]. This cytokine is involved in multiple functions, including host defense and the pathology of autoimmune diseases, infectious diseases, chronic inflammatory diseases, and cancers [7]. IL-17-producing cells may play a role in antitumor immunity. IL-17 is a family of cytokines composed of six members (IL-17A to IL-17F) and five receptors (IL-17RA to IL-17RD and SEF) [8]. Among the IL-17 genes, the best known are IL-17A (rs2275913) and IL-17F (rs7637780), which share the greatest homology between them and encode two common receptors: IL-17RA and IL-17RC [9]. More recent meta-analyses tend to confirm that these polymorphisms are significant risk factors for several cancers [10]. These cells and their cytokines have been implicated in both pro-tumor and anti-tumor processes. They can also promote and exacerbate diseases caused by viruses, as they induce inflammation that affects the microenvironment around tumors, which involves the proliferation, migration, and survival of cancer cells [5]. Although previous studies have evaluated the correlation between the rs3748067 polymorphism of the IL-17A gene and susceptibility to breast and cervical cancer [11][12], the results were inconsistent [5][13]. Furthermore, no studies have been conducted among Burkinabe patients with cervical cancer to evaluate the association with Interleukin-17 polymorphism. The main objective of this study was to analyze the association between IL-17A (rs 2275913) and IL-17F (rs 763780) SNPs and their involvement in the development of cervical cancer in Burkina Faso, as well as their association with HPV in Togo.

 2. Methodology 2.1. Subjects

This is a case-control study conducted from October 2020 to March 2021. The study population in the city of Ouagadougou consisted of 137 sexually active women, including 44 cases of cervical cancer and 93 without a history of cervical cancer. The study population in the city of Kara consisted of 114 sexually active women with no history of cervical cancer. These two populations from two neighboring West African countries have a similarity in HPV genotype mapping. These samples had already been genotyped for HPV [14][15].

 2.2. Nucleic Acid Extraction

Human DNA was extracted using the “R-Biopharm” kit, which allows simultaneous extraction of DNA and RNA, in accordance with the protocols provided by the manufacturers. The nucleic acid extracts obtained were stored at −80˚C for later use.

 2.3. TaqMan Probe Real-Time PCR

Genotyping of the two IL-17 polymorphisms was performed using TaqMan SNP (Applied Biosystems, ABI, Foster City, CA, USA). We used an optimized protocol employed by Yi Quan et al. [5]. For IL-17A rs2275913 genotyping, real-time PCR was performed in a total volume of 10 μL reaction mixture containing 2.5 μL TaqMan (2X) Universal PCR Master Mix, 1 μL of SNP Genotyping Assay (2X), 1.5 μL of sterile DNase-free water, and 5 μL (10 ng) of genomic DNA. The thermal conditions for real-time PCR were 95˚C for 10 min, followed by 49 cycles of 95˚C for 15 s and 60˚C for 1 min. For IL-17F rs763780, real-time PCR with TaqMan probe was performed in a total volume of 10 μL reaction mixture containing 2.5 μL TaqMan PCR Universal Master Mix (2X), 1 μL of SNP genotyping assay (2X), 1.5 μL of sterile DNase-free water, and 5 μL (10 ng) of genomic DNA. The thermal conditions for real-time PCR were 95˚C for 10 minutes, followed by 59 cycles at 95˚C for 15 seconds and 60˚C for 1 minute.

 2.4. Data Analysis

Allele discrimination, allele frequency, and genotype calculations were performed using TaqMan Genotyper® 1.6.0 software. 34. Data were analyzed using Statistical Package for Social Sciences (SPSS) 26.0 and Epi Info version 7.2 software. The chi-square test was used to compare frequencies. Odd ratios and 95% confidence intervals were calculated to assess risk. Results are considered statistically significant for p < 0.05.

 3. Results 3.1. Sociodemographic Characteristics According to HPV Infection in Ouagadougou

Table 1 presents the socio-demographic, behavioral, and sexual characteristics of the 93 sexually active women in Ouagadougou with no history of cervical cancer, according to their HPV status. None of the women smoked. The average age of the participants was 33.91 ± 8.52 years, with ages ranging from 18 to 57 years. There were fewer university graduates and more married women. HPV status was positive in 51 (54.8%) women and negative in 42 (45.2%) women. The number of abortions was statistically associated with infection in Ouagadougou (p-value = 0.007).

Table 1. Sociodemographic, behavioral, and sexual characteristics according to HPV infection among women in Ouagadougou.

Behavioral and sexual characteristics Frequency HPV p-value
HPV − (n) HPV + (n)
Age 0.37
Under 20 years old 1 1 0
20 - 39 70 30 40
40 - 60 22 11 11
Age at first sexual intercourse 0.06
Under 20 years old 62 32 30
Over 20 years 31 10 21
Marital status 0.30
Singles 13 4 9
Brides 78 36 42
Widows 2 2 0
Contraceptive usage 0.94
Yes 35 17 21
No 58 25 30
Gestation 0.41
1 - 5 72 34 45
More than 5 21 08 06
Parity 0.43
0 - 5 80 39 46
More than 5 13 3 5
Number of abortions 0.007
0 64 21 21
1 - 3 29 43 08
Level of education 0.63
Illiterate 27 14 13
Primary school 25 12 13
Secondary school 28 12 16
University 13 4 9
3.2. Sociodemographic Characteristics According to HPV Infection in Kara

Table 2 presents the socio-demographic, behavioral, and sexual characteristics of the 114 participants from the city of Kara according to their HPV status. As in the city of Ouagadougou, none of the women smoked. The average age of the participants was 33.87 ± 9.58 years, with ages ranging from 17 to 61 years. There were fewer university graduates and more married women. The HPV status was 64 (56.14%) women positive for HPV and 42 (43.86%) women negative for HPV. The age of first sexual intercourse was statistically associated with HPV infection in Kara.

Table 2. Sociodemographic, behavioral, and sexual characteristics according to HPV infection in women in Kara.

Behavioral and sexual characteristics Frequency HPV Total p-value
HPV − N (%) HPV + N (%)
Age 114 0.19
Under 20 years old 3 3 0
20 - 39 81 40 41
40 - 60 26 6 20
Over 60 years old 4 1 3
Age at first sexual intercourse 0.048
Under 20 years old 77 30 47 114
Over 20 years 37 20 17
Marital status 0.08
Singles 27 8 19 114
Brides 80 41 39
Widows 7 1 6
Contraceptive usage 0.25
Yes 91 38 53 114
No 23 12 11
Gestation 0.08
1 - 5 104 44 60 114
More than 5 10 6 4
Parity 0.16
0 - 5 109 46 63 114
More than 5 5 4 1
Number of abortions 0.44
0 71 28 43 114
1 - 3 43 22 21
Level of education 0.33
Illiterate 17 10 7 114
Primary school 30 14 16
Secondary school 44 19 25
University 23 7 16
3.3. Distribution of IL-17A Gene Polymorphisms (rs 2275913) According to Socio-Demographic and Sexual Characteristics in Ouagadougou and Kara

Table 3 shows the distribution of IL-17A gene polymorphisms (rs 2275913) in the two cities. Certain characteristics, such as age at first sexual intercourse and parity, were associated with IL-17A (rs 2275913) genotypes in Ouagadougou, and age at first sexual intercourse in Kara. Alleles G and A were not associated with the various characteristics studied in either city.

 3.4. Distribution of Polymorphisms of 1L-17F (rs 763780) and 1L-17A (rs 2275913) Genes in Ouagadougou and Kara

The frequency of genotypes of the rs2275913 polymorphism of the 1L-17A gene was 91.4%, 7.5%, and 1.1% in Ouagadougou, and 91.23%, 7.90%, and 3.23% in Kara, respectively, for the GG, GA, and AA genotypes. Similarly, the frequency of

Table 3. Distribution of polymorphisms of the 1L-17A gene (rs 2275913) in Ouagadougou and Kara.

Characteristics Genotypes p-value Allele p-value
GG AG AA G A
1L-17A (rs 2275913)-Ouagadougou
Age Under 40 years old 66 5 0 0.4 137 5 0.13
More than 40 years 19 2 1 40 4
HPV status Positive 48 3 0 0.42 99 3 0.18
Negative 37 4 1 78 6
Age at first sexual intercourse Under 20 years old 55 5 1 0.005 115 7 0.45
More than 20 years old 29 2 0 60 2
Gestation 0 - 4 66 5 1 0.042 137 7 0.97
More than 4 19 2 0 40 2
Parity 0 - 4 73 5 1 0.07 151 7 0.47
More than 4 11 2 0 24 2
1L-17A (rs 2275913)-Kara
Age 0 - 39 years old 77 6 1 0.98 160 8 0.94
More than 40 years old 27 3 0 57 3
HPV Status Positive 58 6 0 0.42 122 6 0.93
Negative 47 3 1 97 5
Age at first sexual intercourse 0 - 19 years old 70 7 0 0 . 016 147 7 0.77
More than 20 34 2 1 70 4
Gestation 0 - 4 82 8 1 0.98 172 10 0.34
More than 4 22 1 0 45 1
Parity 0 - 4 91 8 1 0.92 190 10 0.74
More than 4 13 1 0 27 1

genotypes of the rs763780 polymorphism of the 1L-17F gene was 74.42%, 19.35% and 3.23% in Ouagadougou and 89.50%, 10.50% and 0.00% in Kara, respectively for the TT, TC and CC genotypes.

The mutation rates of rs 2275913 and rs 763780 polymorphisms were 4.84% and 12.90% for Ouagadougou, respectively, compared to 4.83% and 5.26% for Kara (Table 4).

 3.5. Comparison of Polymorphisms of 1L-17A (rs 2275913) and 1L-17F (rs763780) between CC and Controls in Ouagadougou

Table 5 compares the polymorphisms of 1L-17A (rs 2275913) and 1L-17F (rs763780) between CC patients and controls with no history of CC in Ouagadougou. There is no statistically significant difference between the genotypes of the polymorphisms of 1L-17A (rs 2275913) and 1L-17F (rs763780), cases of cervical cancer, and controls in Ouagadougou in this study.

Table 4. Distribution of polymorphisms of genes 1L-17F (rs 763780) and 1L-17A (rs 2275913) in Ouagadougou and Kara.

Genotypes Effective Frequency (%) Alleles Frequency (%)
Ouagadougou
1L-17A (rs 2275913) GG 85 91.4 G 95.16
GA 7 7.5
AA 1 1.1 A 4.84
Total 93 100
HWE (p-value) 0.79
1L-17F (rs 763780) TT 72 74.42 T 87.10
TC 18 19.35
CC 3 3.23 C 12.9
Total 93 100
HWE (p-value) 0.73
Kara
1L-17A (rs 2275913) GG 104 91.23 G 95.17
GA 9 7.90
AA 1 0.87 A 4.83
Total 114 100
HWE (p-value) 0.81
1L-17F (rs 763780) TT 102 89.5 T 94.74
TC 12 10.5
CC 00 00 C 5.26
Total 114 100
HWE (p-value) 1

Table 5. Comparison of polymorphisms of 1L-17A (rs 2275913) and 1L-17F (rs763780) between CC and controls in Ouagadougou.

Genotypes/ Allele Cervical cancer (%) N = 44 Control (%) N = 93 P -value OR (IC 95%)
rs 2275913
GG 37 (84.10) 85 (91.40) Ref. ---
GA 7 (15.90) 7 (7.52) 0.13 2.29 (0.7 - 7.0)
AA 0 (00) 1 (1.08) NA NA
GA/AA 7 (15.90) 8 (8.60) 0.07 2.62 (0.8 - 7.7)
Allele
G 81 (92.04) 177 (95.16) Ref.
A 7 (8.96) 9 (4.84) 0.30 0.58 (0.2 - 1.6)
rs 763780
TT 34 (77.27) 72 (77.42) Ref.
TC 7 (15.91) 18 (19.35) 0.69 1.21 (0.4 - 3.1)
CC 3 (6.82) 3 (3.23) 0.36 0.47 (0.09 - 2.46)
CT/CC 10 (22.73) 21 (22.58) 0.98 0.99 (0.4 - 2.3)
Allele
T 75 (85.23) 162 (87.10) Ref .
C 13 (14.77) 24 (12.90) 0.67 0.85 (0.4 - 1.7)
3.6. Distribution of Polymorphisms of the 1L-17F Gene (rs763780) According to Characteristics in Ouagadougou and Kara

In the city of Kara, IL-17F (rs763780) genotypes are influenced by characteristics such as gestation and number of abortions. Gestation was associated with the allele type of the IL-17F (rs763780) gene in Ouagadougou. Multi-infection with high-risk oncogenic HPV genotypes, HPV status, and HPV 30S were correlated with IL-17F (rs763780) polymorphism genotypes in Kara. In addition, HPV status significantly affected IL-17F (rs763780) alleles in Kara (Table 6).

Table 6. Distribution of IL-17F gene polymorphisms (rs763780) according to characteristics in Ouagadougou and Kara.

Characteristics Genotypes p-value Allele p-value
TT TC CC T C
IL-17F (rs763780) — Ouagadougou
Age Under 40 years old 55 13 3 0.13 123 19 0.72
More than 40 years old 17 5 0 39 5
HPV Status Positive 37 13 1 0.21 87 15 0.42
Negative 35 5 2 75 9
Age of first sexual intercourse Under 20 years old 46 14 1 0.6 106 16 0.96
More than 20 years old 25 4 2 54 8
Gestation 0 - 4 58 12 2 0.001 128 14 0.02
More than 4 14 6 1 34 10
Parity 0 - 4 64 13 2 0.10 141 17 0.07
More than 4 8 4 1 20 6
Number of abortions Yes 50 12 2 0.05 112 16 0.5
No 22 6 1 30 8
IL-17F (rs763780) — Kara
HPV Genotypes Multi-infection 13 4 0 0.022 30 4 0.31
Single-infection 41 6 0 88 6
HPV Status Positive 54 10 0 0.041 118 10 0.047
Negative 49 2 0 100 2
HPV 30S Yes 13 5 0 0.009 31 5 0.11
No 41 5 0 87 5
Gestation 0 - 4 79 12 0 0.55 170 12 0.07
More than 4 23 0 0 46 0
Parity 0 - 4 88 12 0 0.31 188 12 0.18
More than 4 14 0 0 28 00
3.7. Genotypes and Alleles Frequencies According to the HPV Status in Kara

Table 7 shows the genotype and allele frequencies of the two polymorphisms rs 2275913 and rs763780 in sexually active women in the city of Kara according to HPV status. The rs763780 genotypes were associated with HPV status. The C allele and CT/CC genotypes were favorable to infection with high-risk oncogenic HPV.

Table 7. Genotypic and allelic frequency of rs 2275913 and rs763780 according to HPV status in Kara.

Genotypes/ Allele HPV + (%) N = 64 HPV − (%) N = 51 P -value OR (IC 95%)
rs 2275913
GG 58 (84.10) 47 (91.40) Ref . Ref .
GA 6 (15.90) 3 (7.52) 0,5 0.60 (0.1 - 2.5)
AA 0 (00) 1 (1.08) NA NA
GA/AA 6 (15.90) 4 (8.60) 0.77 0.82 (0.2 - 3.0)
Allele
G 122 (95.31) 97 (95.10) Ref . Ref .
A 6 (4.69) 5 (4.90) 0.93 1.04 (0.3 - 3.5)
rs763780
TT 54 (84.37) 49 (96.08) Ref . Ref .
TC 10 (15.63) 2 (3.92) 0.041 0.22 (0.04 - 1.05)
CC 0 (00) 0 (3.23) NA NA
CT/CC 10 (15.63) 2 (3.92) 0.041 0.22 (0.04 - 1.05)
Allele
T 118 (92.19) 100 (98.04) Ref . Ref .
C 10 (7.81) 2 (1.96) 0.047 0.23 (0.05 - 1.10)

Ref =reference.

 4. Discussion

Cancer is a major global public health issue. In recent years, genetic susceptibility to cancer has attracted increasing attention in the study of genetic polymorphisms in tumorigenesis [16]. The association between inflammation and cytokines plays a key role in the transformation of the epithelium of a precancerous lesion into cervical cancer. Chronic inflammation is a necessary step in maintaining and promoting cancer progression, such as tumor tissue reconstruction, angiogenesis, metastasis, and suppression of the innate anti-cancer immune response [17]. Genetic and epigenetic mutations associated with HPV infection could trigger and maintain cell transformation and autonomous proliferation of transformed cervical cells, leading to cancerous proliferation. Numerous studies conducted in Burkina Faso have shown a high prevalence of HR-HPV likely to progress to cancer, and most of the genotypes found are not covered by the available vaccines [18]-[28]. Furthermore, susceptibility to HPV could be affected by polymorphisms in genes involved in the immune response, which could prevent the progression of cervical lesions, particularly NK cells.

The main objective of this case-control study was to analyze the association between IL-17A (rs 2275913) and IL-17F (rs 763780) SNPs and their involvement in the development of cervical cancer in Burkina Faso, as well as their association with HPV infection in Togo. The results reveal that certain behavioral characteristics of women are involved in the manifestation of IL-17A (rs 2275913) and IL-17F (rs 763780) polymorphisms and HPV infection. Indeed, the age of first sexual intercourse was statistically associated with HPV infection in Kara. This result confirms the study in Burkina Faso, which found a statistically significant association between age at first sexual intercourse and HPV infection [29]. As HPV is mainly transmitted through sexual intercourse, it is important to be aware that early sexual intercourse can be a risk factor for HPV infection [30]. Studies have suggested age as a risk factor for HPV infection, but the age of first sexual intercourse remains a risk factor to be confirmed [26]. Certain characteristics, such as age at first sexual intercourse and pregnancy, were associated with IL-17A genotypes (rs 2275913) in Ouagadougou and age at first sexual intercourse in Kara. Gestation was associated with the allele type of the IL-17F gene (rs 763780) in Ouagadougou. These characteristics are much more strongly associated with HPV infection in Burkina Faso. Unfortunately, several studies on IL-17 in patients with cervical cancer have not reported an association between IL-17 polymorphisms and characteristics such as age at first sexual intercourse, parity, and number of births [13][31][32]. Nevertheless, our results corroborate those of Cong et al., who also found an association between age and primiparity [16]. These results provide a probable indication of the long-term involvement of IL-17 polymorphisms in the progression of cervical cancer.

The G and A alleles were not associated with the various characteristics studied in the two cities. The frequency of genotypes of the rs 2275913 polymorphism of the IL-17A gene was 91.4%, 7.5%, and 1.1% for the GG, GA, and AA genotypes in Ouagadougou, and 91.23%, 7.90%, and 3.23% in Kara, respectively. Similarly, the frequency of genotypes of the rs 763780 polymorphism of the IL-17F gene was 74.42%, 19.35% and 3.23% for the TT, TC and CC genotypes in Ouagadougou and 89.50%, 10.50% and 0.00% respectively in Kara. There was an almost homogeneous distribution of the two IL-17 polymorphisms studied in Ouagadougou and Kara. Elsissy et al. found in a case-control study of an Egyptian population with acute myeloid leukemia proportions of 64%, 22.5% and 13.5% for the TT, TC and CC genotypes for IL-17F respectively, and 30.5%, 16.5%, and 53% for the AA, AG, and GG genotypes of IL-17A, respectively [33]. Although the authors indicated an association between IL-17A and IL-17F polymorphisms and acute myeloid leukemia, these results once again highlight the genetic diversity in Africa. In addition, Quan et al. reported that in a Chinese population with cervical cancer, they found frequencies of 17.3%, 46.4%, and 36.3% for the AA, AG, and GG genotypes of IL-17A, respectively, and 71.4%, 27.3% and 1.3% for the TT, TC and CC genotypes for IL-17F, respectively [5]. The mutation rates for polymorphisms rs 2275913 and rs 763780 were 4.84% and 12. 90% for Ouagadougou, compared to 4.83% and 5.26% for Kara. These mutation rates, which are almost identical in Burkina Faso and Togo, differ from the results of Quan et al. The ethnic mixing, lifestyle, and almost identical climate of the two countries could explain these results.

In our study, there was no statistically significant difference between the genotypes of IL-17A (rs 2275913) and IL-17F (rs 763780) polymorphisms, cervical cancer cases, and controls in Ouagadougou. Quan et al. reported the same results for IL-17F (rs 763780). However, they found a statistically significant association for IL-17A (rs 2275913). Dai et al. found the same results as Quan et al. in a population with cervical cancer [34]. This difference with our results could be explained by the size of our samples and, above all, the number of cancer cases.

Multi-infection with high-risk oncogenic HPV genotypes, HPV status, and HPV 30S were correlated with IL-17F (rs 763780) polymorphism genotypes in Kara. These risk factors contribute significantly to the development of cervical cancer. In addition, HPV status significantly affected IL-17F (rs 763780) alleles in Kara. This variant might influence the immune response to HPV infection.

The rs 763780 genotypes were associated with HPV status, and the C allele and CT/CC genotypes were associated with persistent infection with high-risk oncogenic HPV. Persistent HPV infection is the main cause of cervical cancer. Lv et al. reported results that were partially similar to ours. They suggested that IL-17 was associated with high-risk HPV infection, particularly HPV 16 and HPV 18 [35].

 5. Conclusion

The results of this study indicate that IL-17A and IL-17F polymorphisms are not associated with susceptibility to cervical cancer in our study population. The pathophysiological characteristics of cervical cancer are not affected by the polymorphisms. Certain patient characteristics have an influence on HPV infection and on IL-17A and IL-17F polymorphisms. It would be interesting to continue this study, taking into account certain shortcomings such as the low number of cancer cases and the relatively small size of the study population. These results suggest future research directions for genetic susceptibility to cervical cancer in this population, given that these particular SNPs do not appear to be major risk factors.

 Acknowledgements

We thank the “International Center for Genetic Engineering and Biotechnology (ICGEB)” for funding this research work through the project: “Implication of the host genetic factor in Human Papillomavirus Infection and its associated Cervical Lesions and cancer in West African Women.” Ref. No. CRP/BFA17-01. We also thank the UNESCO chair in “Génie génétique et Biologie Moléculaire” for the technical support.

 Author Contributions

This work was carried out in collaboration among all authors. All authors contributed equally to conception and design of this study. All authors read and approved the final manuscript.

 Ethical Consideration

This study was approved by the National Health Research Ethics Committee of Burkina Faso under No. 2018-01-012.

 References Filho, A.M., Laversanne, M., Ferlay, J., Colombet, M., Piñeros, M., Znaor, A., et al. (2024) The GLOBOCAN 2022 Cancer Estimates: Data Sources, Methods, and a Snapshot of the Cancer Burden Worldwide. InternationalJournalofCancer, 156, 1336-1346. https://doi.org/10.1002/ijc.35278 10.1002/ijc.3527839688499https://doi.org/10.1002/ijc.35278 Filho, A.M. Laversanne, M. Ferlay, J. Colombet, M. Znaor, A. Sources, M 2024 The GLOBOCAN 2022 Cancer Estimates: Data Sources, Methods, and a Snapshot of the Cancer Burden Worldwide International Journal of Cancer 156 10.1002/ijc.35278 39688499 Schlecht, N.F., Kulaga, S., Robitaille, J., Ferreira, S., Santos, M., et al. (2001) Persistent Human Papillomavirus Infection as a Predictor of Cervical Intraepithelial Neoplasia. Journal of theAmericanMedicalAssociation, 286, 3106-3114. https://doi.org/10.1001/jama.286.24.3106 10.1001/jama.286.24.310611754676https://doi.org/10.1001/jama.286.24.3106 Schlecht, N.F. Kulaga, S. Robitaille, J. Ferreira, S. Santos, M. 2001 Persistent Human Papillomavirus Infection as a Predictor of Cervical Intraepithelial Neoplasia Journal of the American Medical Association 286 10.1001/jama.286.24.3106 11754676 Duport, N. (2008) Données épidémiologiques sur le cancer du col de l’utérus—État des connaissances-Actualisation 2008. Institut de Veille Sanitaire, France. Duport, N. Sanitaire, F 2008 Données épidémiologiques sur le cancer du col de l’utérus—État des connaissances-Actualisation 2008 Institut de Veille Sanitaire Yang, J., Wang, Y., Zhang, S., Li, Y., Li, C., Liu, W., et al. (2022) The Association of TNF- α Promoter Polymorphisms with Genetic Susceptibility to Cervical Cancer in a Chinese Han Population. International Journal of General Medicine, 15, 417-427. https://doi.org/10.2147/ijgm.s350263 10.2147/ijgm.s35026335046703https://doi.org/10.2147/ijgm.s350263 Yang, J. Wang, Y. Zhang, S. Li, Y. Li, C. Liu, W. 2022 The Association of TNF-α Promoter Polymorphisms with Genetic Susceptibility to Cervical Cancer in a Chinese Han Population International Journal of General Medicine 15 10.2147/ijgm.s350263 35046703 Quan, Y., Zhou, B., Wang, Y., Duan, R., Wang, K., Gao, Q., et al. (2012) Association between IL17 Polymorphisms and Risk of Cervical Cancer in Chinese Women. Clinical and Developmental Immunology, 2012, 1-6. https://doi.org/10.1155/2012/258293 10.1155/2012/25829323049595https://doi.org/10.1155/2012/258293 Quan, Y. Zhou, B. Wang, Y. Duan, R. Wang, K. Gao, Q. 2012 Association between IL17 Polymorphisms and Risk of Cervical Cancer in Chinese Women Clinical and Developmental Immunology 2012 10.1155/2012/258293 23049595 Yang, S., Li, C., Li, X., Huang, X., Zhao, Q., Liu, D., et al. (2017) Relationship of IL-17A and IL-17F Genetic Variations to Cervical Cancer Risk: A Meta-Analysis. Biomarkers in Medicine, 11, 459-471. https://doi.org/10.2217/bmm-2016-0315 10.2217/bmm-2016-031528621613https://doi.org/10.2217/bmm-2016-0315 Yang, S. Li, C. Li, X. Huang, X. Zhao, Q. Liu, D. 2017 Relationship of IL-17A and IL-17F Genetic Variations to Cervical Cancer Risk: A Meta-Analysis Biomarkers in Medicine 11 10.2217/bmm-2016-0315 28621613 Iwakura, Y., Ishigame, H., Saijo, S. and Nakae, S. (2011) Functional Specialization of Interleukin-17 Family Members. Immunity, 34, 149-162. https://doi.org/10.1016/j.immuni.2011.02.012 10.1016/j.immuni.2011.02.01221349428https://doi.org/10.1016/j.immuni.2011.02.012 Iwakura, Y. Ishigame, H. Saijo, S. Nakae, S. 2011 Functional Specialization of Interleukin-17 Family Members Immunity 34 10.1016/j.immuni.2011.02.012 21349428 Kawaguchi, M., Adachi, M., Oda, N., Kokubu, F. and Huang, S. (2004) IL-17 Cytokine Family. JournalofAllergyandClinicalImmunology, 114, 1265-1273. https://doi.org/10.1016/j.jaci.2004.10.019 10.1016/j.jaci.2004.10.01915577820https://doi.org/10.1016/j.jaci.2004.10.019 Kawaguchi, M. Adachi, M. Oda, N. Kokubu, F. Huang, S. 2004 IL-17 Cytokine Family Journal of Allergy and Clinical Immunology 114 10.1016/j.jaci.2004.10.019 15577820 Niu, A.Q., Cao, Y.H., Wang, H., Zhang, X., Zhu, B. and Li, Z.H. (2017) Role of IL17A rs2275913 and rs3748067 Polymorphisms in the Risk Cervical Cancer. Genetics and Molecular Research, 16, 1-9. Niu, A.Q. Cao, Y.H. Wang, H. Zhang, X. Zhu, B. Li, Z.H. 2017 Role of IL17A rs2275913 and rs3748067 Polymorphisms in the Risk Cervical Cancer Genetics and Molecular Research 16 Liu, T., Yang, L., Lv, X., Zuo, C., Jia, C., Yang, Z., et al. (2022) Cumulative Evidence for Associations between Genetic Variants in Interleukin 17 Family Gene and Risk of Human Diseases. Frontiers in Immunology, 13, Article 1008184. https://doi.org/10.3389/fimmu.2022.1008184 10.3389/fimmu.2022.100818436300118https://doi.org/10.3389/fimmu.2022.1008184 Liu, T. Yang, L. Lv, X. Zuo, C. Jia, C. Yang, Z. 2022 Cumulative Evidence for Associations between Genetic Variants in Interleukin 17 Family Gene and Risk of Human Diseases Frontiers in Immunology 13 1008184 10.3389/fimmu.2022.1008184 36300118 Aziz, M.A., Chowdhury, S., Jafrin, S., Barek, M.A., Uddin, M.S., Millat, M.S., et al. (2024) Genetic Association of Interleukin-17A Polymorphism in Bangladeshi Patients with Breast and Cervical Cancer: A Case-Control Study with Functional Analysis. BMC Cancer, 24, Article No. 660. https://doi.org/10.1186/s12885-024-12352-0 10.1186/s12885-024-12352-038816694https://doi.org/10.1186/s12885-024-12352-0 Aziz, M.A. Chowdhury, S. Jafrin, S. Barek, M.A. Uddin, M.S. Millat, M.S. 2024 Genetic Association of Interleukin-17A Polymorphism in Bangladeshi Patients with Breast and Cervical Cancer: A Case-Control Study with Functional Analysis BMC Cancer 24 No 10.1186/s12885-024-12352-0 38816694 Hu, Y., Xu, D., Xia, H., Zhang, M. and Liang, C. (2021) Associations of IL-17A-197G/A and IL-17F 7488T/C Polymorphisms with Cancer Risk in Asians: An Updated Meta-Analysis from 43 Studies. Gene, 804, Article 145901. https://doi.org/10.1016/j.gene.2021.145901 10.1016/j.gene.2021.14590134403774https://doi.org/10.1016/j.gene.2021.145901 Hu, Y. Xu, D. Xia, H. Zhang, M. Liang, C. 2021 Associations of IL-17A-197G/A and IL-17F 7488T/C Polymorphisms with Cancer Risk in Asians: An Updated Meta-Analysis from 43 Studies Gene 804 145901 10.1016/j.gene.2021.145901 34403774 Li, L., Tian, Y.L., Lv, X.M., Yu, H.F., Xie, Y.Y., Wang, J.D., et al. (2015) Association Analysis of IL-17A and IL-17F Polymorphisms in Chinese Women with Cervical Cancer. Genetics and Molecular Research, 14, 12178-12183. https://doi.org/10.4238/2015.october.9.6 10.4238/2015.october.9.626505366https://doi.org/10.4238/2015.october.9.6 Li, L. Tian, Y.L. Lv, X.M. Yu, H.F. Xie, Y.Y. Wang, J.D. 2015 Association Analysis of IL-17A and IL-17F Polymorphisms in Chinese Women with Cervical Cancer Genetics and Molecular Research 14 10.4238/2015.october.9.6 26505366 Dolou, E.D., Kuassi-Kpede, A., M Zohoncon, T., Marie Traore, I., Katawa, G., R Ouedraogo, A., et al. (2021) Molecular Characterization of High-Risk Humanpapillomavirus Genotypes in Women with or without Cervical Lesions at VIA/VILI in Kara, Togo. African Health Sciences, 21, 1715-1721. https://doi.org/10.4314/ahs.v21i4.27 10.4314/ahs.v21i4.2735283946https://doi.org/10.4314/ahs.v21i4.27 Dolou, E.D. Kuassi-Kpede, A. Zohoncon, T. Traore, I. Katawa, G. Ouedraogo, A. Kara, T 2021 Molecular Characterization of High-Risk Humanpapillomavirus Genotypes in Women with or without Cervical Lesions at VIA/VILI in Kara, Togo African Health Sciences 21 10.4314/ahs.v21i4.27 35283946 Zohoncon, T.M., Djigma, W.F., Ouattara, A.K., Traore, I.M.A., et al. (2020) Mapping of Fourteen High-Risk Human Papillomavirus Genotypes by Molecular Detection in Sexually Active Women in the West African Sub-Region. International Journal of Genetics and Molecular Biology, 12, 11-21. Zohoncon, T.M. Djigma, W.F. Ouattara, A.K. Traore, I.M.A. 2020 Mapping of Fourteen High-Risk Human Papillomavirus Genotypes by Molecular Detection in Sexually Active Women in the West African Sub-Region International Journal of Genetics and Molecular Biology 12 Cong, J., Liu, R., Wang, X., Sheng, L., et al. (2015) Association between Interluekin-17 Gene Polymorphisms and the Risk of Cervical Cancer in a Chinese Population. International Journal of Clinical and Experimental Pathology, 8, 9567-73. https://pmc.ncbi.nlm.nih.gov/articles/PMC4583952/ Cong, J. Liu, R. Wang, X. Sheng, L. 2015 Association between Interluekin-17 Gene Polymorphisms and the Risk of Cervical Cancer in a Chinese Population International Journal of Clinical and Experimental Pathology 8 Chechlinska, M., Kowalewska, M. and Nowak, R. (2010) Systemic Inflammation as a Confounding Factor in Cancer Biomarker Discovery and Validation. Nature Reviews Cancer, 10, 2-3. https://doi.org/10.1038/nrc2782 10.1038/nrc278220050335https://doi.org/10.1038/nrc2782 Chechlinska, M. Kowalewska, M. Nowak, R. 2010 Systemic Inflammation as a Confounding Factor in Cancer Biomarker Discovery and Validation Nature Reviews Cancer 10 10.1038/nrc2782 20050335 Djigma, F.W., Ouédraogo, C., Karou, D.S., Sagna, T., Bisseye, C., Zeba, M., et al. (2011) Prevalence and Genotype Characterization of Human Papillomaviruses among HIV-Seropositive in Ouagadougou, Burkina Faso. Acta Tropica, 117, 202-206. https://doi.org/10.1016/j.actatropica.2010.12.007 10.1016/j.actatropica.2010.12.00721167118https://doi.org/10.1016/j.actatropica.2010.12.007 Djigma, F.W. Karou, D.S. Sagna, T. Bisseye, C. Zeba, M. Ouagadougou, B 2011 Prevalence and Genotype Characterization of Human Papillomaviruses among HIV-Seropositive in Ouagadougou, Burkina Faso Acta Tropica 117 10.1016/j.actatropica.2010.12.007 21167118 Kabre, K.M., Ouermi, D., Zohoncon, T.M., Traore, F.P.W., Gnoumou, O.P.D.P., Ouedraogo, R.A., et al. (2022) Molecular Epidemiology of Human Papillomavirus in Pregnant Women in Burkina Faso. Biomolecular Concepts, 13, 334-340. https://doi.org/10.1515/bmc-2022-0026 10.1515/bmc-2022-002636727673https://doi.org/10.1515/bmc-2022-0026 Kabre, K.M. Ouermi, D. Zohoncon, T.M. Traore, F.P.W. Gnoumou, O.P.D.P. Ouedraogo, R.A. 2022 Molecular Epidemiology of Human Papillomavirus in Pregnant Women in Burkina Faso Biomolecular Concepts 13 10.1515/bmc-2022-0026 36727673 Ouedraogo, C.M., Rahimy, R.M., Zohoncon, T.M., et al. (2015) Epidemiology and Characterization of High-Risk Genotypes of Human Papillomavirus in a Population of Sexually Active Adolescents in Ouagadougou. Journal de gynécologie, obstétrique et biologie de la reproduction, 44, 715-722. Ouedraogo, C.M. Rahimy, R.M. Zohoncon, T.M. 2015 Epidemiology and Characterization of High-Risk Genotypes of Human Papillomavirus in a Population of Sexually Active Adolescents in Ouagadougou Journal de gynécologie 44 Ouedraogo, R.A., Zohoncon, T.M., Traore, I.M.A., Ouattara, A.K., Guigma, S.P., Djigma, F.W., et al. (2020) Genotypic Distribution of Human Oncogenic Papillomaviruses in Sexually Active Women in Burkina Faso: Central, Central-Eastern and Hauts-Bassins Regions. Biomolecular Concepts, 11, 125-136. https://doi.org/10.1515/bmc-2020-0011 10.1515/bmc-2020-001132417758https://doi.org/10.1515/bmc-2020-0011 Ouedraogo, R.A. Zohoncon, T.M. Traore, I.M.A. Ouattara, A.K. Guigma, S.P. Djigma, F.W. Central, C 2020 Genotypic Distribution of Human Oncogenic Papillomaviruses in Sexually Active Women in Burkina Faso: Central, Central-Eastern and Hauts-Bassins Regions Biomolecular Concepts 11 10.1515/bmc-2020-0011 32417758 Sagna, T., Djigma, F., Zeba, M., Bisseye, C., Karou, S.D., Ouermi, D., et al. (2010) Human Papillomaviruses Prevalence and Genital Co-Infections in HIV-Seropositive Women in Ouagadougou (Burkina Faso). Pakistan Journal of Biological Sciences, 13, 951-955. https://doi.org/10.3923/pjbs.2010.951.955 10.3923/pjbs.2010.951.95521313918https://doi.org/10.3923/pjbs.2010.951.955 Sagna, T. Djigma, F. Zeba, M. Bisseye, C. Karou, S.D. Ouermi, D. 2010 Human Papillomaviruses Prevalence and Genital Co-Infections in HIV-Seropositive Women in Ouagadougou (Burkina Faso) Pakistan Journal of Biological Sciences 13 10.3923/pjbs.2010.951.955 21313918 Salambanga, C., Zohoncon, T.M., Traore, I.M.A., et al. (2019) High Prevalence of High-Risk Human Papillomavirus (HPV) Infection among Sexually Active Women in Ouagadougou. Médecine et Santé Tropicales, 29, 302-305. Salambanga, C. Zohoncon, T.M. Traore, I.M.A. 2019 High Prevalence of High-Risk Human Papillomavirus (HPV) Infection among Sexually Active Women in Ouagadougou Médecine et Santé Tropicales 29 Traore, I.M.A., Zohoncon, T.M., Dembele, A., Djigma, F.W., Obiri-Yeboah, D., Traore, G., et al. (2016) Molecular Characterization of High-Risk Human Papillomavirus in Women in Bobo-Dioulasso, Burkina Faso. BioMedResearchInternational, 2016, 1-6. https://doi.org/10.1155/2016/7092583 10.1155/2016/709258327525275https://doi.org/10.1155/2016/7092583 Traore, I.M.A. Zohoncon, T.M. Dembele, A. Djigma, F.W. Obiri-Yeboah, D. Traore, G. Bobo-Dioulasso, B 2016 Molecular Characterization of High-Risk Human Papillomavirus in Women in Bobo-Dioulasso, Burkina Faso BioMed Research International 2016 10.1155/2016/7092583 27525275 Trore, M.A.E., Djigma, W.F., Setor, A.M., Zohoncon, T.M., et al. (2019) MSPI Polymorphisms (Rs4646903) of the Cyp1a1 Gene in Women Infected with Human Papillomavirus in West Africa. International Journal of Current Research, 11, 8390-8396. Trore, M.A.E. Djigma, W.F. Setor, A.M. Zohoncon, T.M. 2019 MSPI Polymorphisms (Rs4646903) of the Cyp1a1 Gene in Women Infected with Human Papillomavirus in West Africa International Journal of Current Research 11 Zabre, P., Sagna, T., Ouedraogo, R. and Simpore, J. (2024) Epidemiological Profile of Human Papillomavirus Infections and Cervical Cancer Prevention among Sexually Active Women in Burkina Faso: Literature Review. MedicalResearchArchives, 12, 1-17. https://doi.org/10.18103/mra.v12i11.5883 10.18103/mra.v12i11.5883https://doi.org/10.18103/mra.v12i11.5883 Zabre, P. Sagna, T. Ouedraogo, R. Simpore, J. 2024 Epidemiological Profile of Human Papillomavirus Infections and Cervical Cancer Prevention among Sexually Active Women in Burkina Faso: Literature Review Medical Research Archives 12 10.18103/mra.v12i11.5883 Zohoncon, T.M., Bisseye, C., Djigma, F.W., Yonli, A.T., et al. (2013) Prevalence of HPV High-Risk Genotypes in Three Cohorts of Women in Ouagadougou (Burkina Faso). Mediterranean Journal of Hematology and Infectious Diseases, 5, e2013059. Zohoncon, T.M. Bisseye, C. Djigma, F.W. Yonli, A.T. 2013 Prevalence of HPV High-Risk Genotypes in Three Cohorts of Women in Ouagadougou (Burkina Faso) Mediterranean Journal of Hematology and Infectious Diseases 5 Zohoncon, T.M., Bado, P. Ouermi, D., et al. (2016) Molecular Characterization of High-Risk Human Papillomavirus Genotypes Involved in Invasive Cervical Cancer from Formalin-Fixed, Paraffin-Embedded Tissues in Ouagadougou, Burkina Faso. International Journal of Current Research, 8, 39314-39318. https://www.journalcra.com/sites/default/files/issue-pdf/17856.pdf Zohoncon, T.M. Bado, P. Ouermi, D. Formalin-Fixed, P Ouagadougou, B 2016 Molecular Characterization of High-Risk Human Papillomavirus Genotypes Involved in Invasive Cervical Cancer from Formalin-Fixed, Paraffin-Embedded Tissues in Ouagadougou, Burkina Faso International Journal of Current Research 8 Maria, H., Dana, H., Françoise, M., Michael, P. and Jürgen, W. (2018) Human Papillomaviruses in Western Africa: Prevalences and Risk Factors in Burkina Faso. ArchivesofGynecologyandObstetrics, 298, 789-796. https://doi.org/10.1007/s00404-018-4860-z 10.1007/s00404-018-4860-z30120545https://doi.org/10.1007/s00404-018-4860-z Maria, H. Dana, H. Michael, P. 2018 Human Papillomaviruses in Western Africa: Prevalences and Risk Factors in Burkina Faso Archives of Gynecology and Obstetrics 298 10.1007/s00404-018-4860-z 30120545 Gavillon, N., Vervaet, H., Derniaux, E., et al. (2010) How Did I Contract Human Papillomavirus (HPV)? Gynecologie, obstetrique & fertilite, 38, 199-204. Gavillon, N. Vervaet, H. Derniaux, E. 2010 How Did I Contract Human Papillomavirus (HPV)? Gynecologie, obstetrique & fertilite, 38, 199-204 Punt, S., Fleuren, G.J., Kritikou, E., Lubberts, E., Trimbos, J.B., Jordanova, E.S., et al. (2015) Angels and Demons: Th17 Cells Represent a Beneficial Response, While Neutrophil IL-17 Is Associated with Poor Prognosis in Squamous Cervical Cancer. OncoImmunology, 4, e984539. https://doi.org/10.4161/2162402x.2014.984539 10.4161/2162402x.2014.98453925949866https://doi.org/10.4161/2162402x.2014.984539 Punt, S. Fleuren, G.J. Kritikou, E. Lubberts, E. Trimbos, J.B. Jordanova, E.S. Response, W 2015 Angels and Demons: Th17 Cells Represent a Beneficial Response, While Neutrophil IL-17 Is Associated with Poor Prognosis in Squamous Cervical Cancer OncoImmunology 4 10.4161/2162402x.2014.984539 25949866 Sun, L.X., Wang, X.B. and Huang, X.J. (2015) Association Analysis of rs2275913G>A and rs763780T>C Interleukin 17 Polymorphisms in Chinese Women with Cervical Cancer. Genetics and Molecular Research, 14, 13612-13617. Sun, L.X. Wang, X.B. Huang, X.J. 2015 Association Analysis of rs2275913G>A and rs763780T>C Interleukin 17 Polymorphisms in Chinese Women with Cervical Cancer Genetics and Molecular Research 14 Elsissy, M., Abdelhafez, A., Elmasry, M. and Salah, D. (2019) Interleukin-17 Gene Polymorphism Is Protective against the Susceptibility to Adult Acute Myeloid Leukaemia in Egypt: A Case-Control Study. OpenAccessMacedonianJournalofMedicalSciences, 7, 1425-1429. https://doi.org/10.3889/oamjms.2019.306 10.3889/oamjms.2019.30631198448https://doi.org/10.3889/oamjms.2019.306 Elsissy, M. Abdelhafez, A. Elmasry, M. Salah, D. 2019 Interleukin-17 Gene Polymorphism Is Protective against the Susceptibility to Adult Acute Myeloid Leukaemia in Egypt: A Case-Control Study Open Access Macedonian Journal of Medical Sciences 7 10.3889/oamjms.2019.306 31198448 Dai, Z.M., Zhang, T.S., Lin, S., Zhang, W.G., et al. (2016) Role of IL-17A rs2275913 and IL-17F rs763780 Polymorphisms in Risk of Cancer Development: An Updated Meta-Analysis. ScientificReports, 6, Article No. 20439. https://doi.org/10.1038/srep20439 10.1038/srep2043926843459https://doi.org/10.1038/srep20439 Dai, Z.M. Zhang, T.S. Lin, S. Zhang, W.G. 2016 Role of IL-17A rs2275913 and IL-17F rs763780 Polymorphisms in Risk of Cancer Development: An Updated Meta-Analysis Scientific Reports 6 No 10.1038/srep20439 26843459 Lv, Q., Zhu, D., Zhang, J., Yi, Y., Yang, S. and Zhang, W. (2015) Association between Six Genetic Variants of IL-17A and IL-17F and Cervical Cancer Risk: A Case-Control Study. TumorBiology, 36, 3979-3984. https://doi.org/10.1007/s13277-015-3041-y 10.1007/s13277-015-3041-y25596084https://doi.org/10.1007/s13277-015-3041-y Lv, Q. Zhu, D. Zhang, J. Yi, Y. Yang, S. Zhang, W. 2015 Association between Six Genetic Variants of IL-17A and IL-17F and Cervical Cancer Risk: A Case-Control Study Tumor Biology 36 10.1007/s13277-015-3041-y 25596084