The study area is situated in Maojiashan (formerly konwn as Liyuan Village, loccated at 104˚26'N, 30˚38'E), within Shanquan Town, Longquanyi District, Chengdu City. It is nestled in the core of the Longquan Mountain range. The climate in this region is temperate, featuring four distinct seasons, and falls under the category of a subtropical monsoon humid climate. Annual rainfall varies between 800 to 1100 mm, with the rainy season predominantly occurring from June to September, contributing to 85% of the annual rainfall. January and December constitute the dry seasons, characterized by lower precipitation, dry winters, and spring droughts. The frost-free period lasts spans 280 days. The soil comprises neutral purple soil derived from slate and shale, possessing a depth exceeding 60 cm.

The newly established peach orchard was previously a forested area. In the middle and late January of 2022, soil improvement measures were taken in the newly established peach orchard by means of deep soil tillage and the application of bio-organic fertilizer at a rate of 45 tons per hectare. Golden Honey Peach No. 1, provided by Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, was selected as the experimental material, and fruit seedlings were cultivated in late February. In February 2023, six fertilization treatments were established in the peach orchard, including 1) conventional management with bio-organic fertilizer (CK) from Sichuan Tianbao Biotechnology Co., Ltd, China, 2) Application of rapeseed meal cake fertilizer (RMC) with an organic content of 80% (9375 kg·ha⁻¹). 3) Planting glabrous hairy vetch (Viciavillosa var. glabrescens) as green manure (GM) at s sowing rate of 45 kg·ha⁻¹. 4) Spraying selenium fertilizer (SF) provided by Agricultural Resources and Environment Research Institute, Guangxi Academy of Agricultural Sciences. The selenium-rich biological nano-grade amino acid-based nutrient solution containing 0.2% selenium was mixed with 70-100 times of water and sprayed through the spraying system 1 - 2 times during the young fruit stage, with a 5 - 7 day interval if sprayed twice [30]. 5) A combination of planting glabrous hairy vetch and rapeseed meal cake fertilizer (GM + RMC), wit half the application amounts of GM and RMC compared to their individual treatments. 6) A combination of selenium fertilizer and rapeseed meal cake fertilizer (SF + RMC), with half the application amounts of SF and RMC compared to their individual treatments. Each treatment has three replicates. The fertilization and management methods were as follows: conventional management with bio-organic fertilizer at 500 kg·ha⁻¹ served as the control (CK). For the GM + RMC and SF + RMC treatments, the application rates of RMC, GM, and SF were reduced to the half of those used in their respective single treatments. All other management and site conditions were consistent across treatments.

In mid-June 2023, soil samples were collected using a soil auger with a 5 cm diameter, reaching a depth of 15 cm. An S-shaped sampling pattern, encompassing 8 - 10 points, was employed, and the collected soil samples were subsequently combined into a single composite sample. Each treatment involved 3 replicates. To prepare the samples for analysis, they were then sieved through a 10-mesh sieve to eliminate roots and stones. One portion of the sieved soil was transported back to the laboratory in an ice box for soil nematode testing, while another portion was retained for measuring the soil’s chemical properties. The soil bulk density was determined using the cutting ring method, and soil moisture content (%) at the sampling points was measured using a soil parameter meter (TDR200). The assessment of soil chemical properties includes the determination of soil pH, soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), total potassium (TK), available phosphorus (AP), available potassium (AK), NH 4 + -N , and NO 3 − -N . The specific methodologies employed for these measurements are referenced in Bao [31].

A total of 100 g fresh soil underwent processing via the Baermann funnel method [32], and after 48 hours, a separation experiment was conducted to isolate soil nematodes. Approximately 10 ml nematode solution was collected, and the total DNA of the nematode community was extracted following the instructions provided by the soil DNA kit manufactured by Omega Bio-tek (Norcross, GA, USA). The quality of the extracted DNA was verified using 1% agarose gel electrophoresis, while its concentration and purity of the DNA were measured using a NanoDrop 2000 spectrophotometer. Subsequently, the soil DNA extract was preamplified using primers NeMF (5’-GGGGAAGTATGGTT GCAAA-3’) and 18Sr2b (5’-TACAAAGGGCAGGGACGTAAT-3’). This was followed by PCR amplification of the nematode 18S rDNA sequence using primers NF1 F (5’-GGTGGTGCATGGCCGTTC TTATT-3’) and 18Sr2bR (5’-TACAAAGGGCAG-GGACGTAAT-3’) [33]. Sequencing was carried out using the Miseq PE300 platform by Shanghai Majorbio Pharmaceutical Technology Co., Ltd. The raw sequencing data underwent quality control using fastp software version 0.20.0 [34], and assembly was performed using FLASH software version 1.2.7 [35]. These sequences were then clustered into Operational Taxonomic Units (OTUs) based on 97% similarity using UPARSE software version 7.1 [36]. After quality control and assembly, the optimized sequences were further processed using the Delber plugin in the QIIME II pipeline [37] to remove sequences annotated to chloroplasts and mitochondria. To reduce the influence of sequencing depth on subsequent diversity analysis, the sequence count in all samples was normalized to the minimum sample sequence count. Taxonomic information was obtained by referencing the PR² sequence database.

The Reaction Mixture consisted of 2X ChamQ SYBR Color qPCR Master Mix (5 µl) from Vazyme Biotech Co., Ltd. (Nanjing, China), 0.4 µl of each primer (5uM), 0.2 µl of 50 X ROX Reference Dye, 1 µl of template DNA, and ddH₂O to adjust the total volume to 10 µl. The PCR program was set as follows: an initial denaturation step at 95˚C for 3 minutes, followed by cycles of denaturation at 95˚C for 5 seconds, annealing at 58˚C for 30 seconds, and extension at 72˚C for 1 minute. After completing these steps, the prepared samples in a 96-well plate were placed in an ABI 7300 Real-Time Fluorescent Quantitative PCR Instrument (Applied Biosystems, USA) to initiate the reaction. Once the constructed plasmid was confirmed to be correct through sequencing, the OD260 value of the plasmid was measured using an ultraviolet spectrophotometer (NanoDrop2000, Thermo Fisher Scientific, USA), and the copy number (copies/μl) was subsequently calculated employing a designated formula. Based on the genera from OTU taxon, combined with the databse http://Nemaplex.ucdavis.edu, a total of 15 nematodes were identified at the genus level in the peach orchards (TableS1). The fresh weight of these nematodes was estimated by multiplying their respective OTU abundances, determined through qPCR, by their fresh body mass. The body mass data were sourced from Zhao et al. [38] and a publicly accessible database located at http://Nemaplex.ucdavis.edu.

The Shannon-Wiener (H’) index was calculated as described in Gao et al. [39] to assess nematode diversity. Furthermore, based on the feeding habits, nematodes are categorized into four trophic groups: bacterivores, fungivores, omnivores-predators, and plant parasites [40]. Additionally, they were grouped into five c-p categories, spanning from r-strategists to k-strategists, labeled c-p1 through c-p5 [41]. In the study, key ecological nematode indices, including the nematode maturity index (MI), plant parasite index (PPI), enrichment index (EI), structural index (SI), basic index (BI), and channel index (CI), were calculated [41]-[44]. The nematode maturity index (MI) and plant parasite index (PPI) are used to effectively gauge environmental perturbations and are calculated as follows:

where v(i) is the cp value of taxon i, and f(i) is the frequency of each taxon and f(i') is the frequency of plant parasites [41][42]. The EI measures how opportunistic bacterial feeders (Ba1 and Ba2) and fungal feeders (Fu2) react to changes in food availability and nutrient enrichment [43]. The SI offers a way to assess the complexity of the soil food web, with higher values indicating a well-structured and stable ecosystem [43]. The BI, which focuses on the abundance of nematodes that feed on bacteria and fungi within the cp grouping, can indicate poor soil health when values are high [43][45]. Finally, the CI provides insight into the decomposition pathways within the soil food web, with high values suggesting that fungal-feeding nematodes are the primary drivers of organic matter decomposition, while low values indicate a greater dominance of bacterial-driven decomposition [43]. The four indices were calculated as follows [43]:

where e represents the weighted frequencies of enrichment component, specifically Ba1 and Fu2, bdenotes the weighted frequencies of basal component, comprising Ba2 and Fu2, and scorresponds to the weighted frequencies of structural component, which encompass Ba₃-Ba₄, Fu₃-Fu₄, Om₃-Om₅, and Pr₂-Pr₅ nematodes. The trophic groups are labeled as Ba for bacterivores, Fu for fungivores, Pr for predators, and Om for omnivores, with each group further differentiated by specific numbers indicating their respective cp values [44].

The nematode metabolic footprint (NMF) analysis served as a tool to assess the nematode-meidated carbon and energy flows, ultimately evaluating the nematode community based on the combined area of the enrichment and structural footprints [44]. The NMF results were presented through rhombuses, specifically at the following coordinates: (SI − 0.5*Fs/k, EI), (SI, EI + 0.5*Fe/k), (SI + 0.5*Fs/k, EI), and (SI, EI − 0.5*Fe/k) [44]. In this context, Fs and Fe represent the structure and enrichment footprints, respectively, with k being a constant value of 3 [43]. The Fs, Fe, and other nematode indices were computed using the NINJA website (https://sieriebriennikov.shinyapps.io/ninja/) [46]. Furthermore, faunal analysis entails constructing a four-quadrant diagram (illustrated in Figure 3) by plotting the enrichment index (EI) against the structural index (SI). This diagram visually represents key attributes that reflect the stability of the soil food web and the availability of resources [44].

Before conducting the statistical analysis, all data were tested for normality and homogeneity of variances, and those that did not meet the criteria underwent natural logarithmic transformation, square root transformation, or rank transformation. The effects of different fertilization treatments on soil physico-chemical properties, nematode abundance, diversity, and ecological indices were analyzed using a one-way ANOVA. To determine significant differences among variables across various treatments, the Least Significant Different (LSD) test was employed, with a significance level set at 0.05. To further explore relationships, Canonical Correspondence Analysis (CCA) was conducted to evaluate the associations between the proportion of each genus-level taxon and environmental variables. Additionally, Redundancy Analysis (RDA) was employed to examine the correlations between the abundance of functional groups and environmental factors. The ANOVA was carried out using SPSS 20.0, while the CCA and RDA analyses were conducted with Canoco 5.0.

Fertilization measures have diverse effects on soil properties (Table 1). Specifically, soil bulk density was notably lower in the green manure (GM) treatment compared to the other five treatments. Furthermore, the available phosphorus (AP) content in GM was significantly higher than in other treatments. Soil pH values were significantly elevated in the rapeseed meal cake fertilizer (RMC) and selenium fertilizer + rapeseed meal cake fertilizer (SF + RMC) treatments than compared to the other four treatments. The total nitrogen (TK) content in the control (CK) and RMC treatments was significantly higher than in the SF + RMC treatment. Ammonium nitrogen ( NH 4 + -N ) levels in CK were significantly higher than in the other five treatments, whereas nitrate nitrogen ( NO 3 − -N ) levels in selenium fertilizer (SF) were significantly lower than in the other five treatments. Available potassium (AK) in CK, RMC, GM, and SF was significantly higher than in the GM + RMC and SF + RMC treatments. However, soil organic carbon content under different fertilization treatments did not reach a significant level (p > 0.05).

Table1. Soil properties under different fertilization measures in the peach orchard (mean ± SE).

Note: CK, control; RMC, rapeseed meal cake fertilization; GM, interplanting a green manure Viciavillosa; SF, selenium fertilization; GM + RMC, a measure combined with green manure and rapeseed meal cake fertilizer; SF + RMC, a measure combined selenium fertilizer and rapeseed meal cake fertilizer. Bulk density, soil bulk density; SOC, soil organic matter; TN, total nitrogen; TP, total phosphorus; TK, total potassium; AP, available phosphorus; AK, available potassium; NH 4 + -N , ammonium nitrogen; NO 3 − -N , nitrate nitrogen. Different letters in the same row indicate significant differences between treatments at levels of p < 0.05.

A total of 15 genera were recorded across all the peach orchard (Table S1), with Acrobeloides and Aphelenchus being the dominant genera. The abundance of soil nematode varied considerably among the different fertilization treatments (Table S1). Specifically, the highest total nematode abundance was observed in the GM + RMC treatment, reaching 3.58 × 10⁸ copies per 100 g of dry soil. Compared to the CK treatment, the total nematode abundance in the GM + RMC treatment increased by 399.11%, while it was 1.74% - 58.91% lower in the other four treatments (Figure 1(a)). In terms of soil nematode diversity, the SF treatment exhibited a significantly higher diversity compared to other fertilization measures (p < 0.05, Figure 1(b)). The abundance of trophic groups also varied across the peach orchards, with bacterivores ranging from 1.45 × 10⁶ to 2.62 × 10⁸ copies, fungivores from 1.97 × 10⁶ to 6.35 × 10⁷ copies, plant parasites from 0 to 3.24 × 10⁷ copies, and omnivores-predators from 0 to 1.71 × 10⁷ copies per 100 g of dry soil (Table 2). Furthermore, the GM + RMC treatment showed a notably greater abundance of bacterivores compared to the other five treatments (p < 0.05). However, no significant differences in the abundance of fungivores, herbivores and omnivores-predators were observed among these fertilization treatments (Table 2).

Figure 1. Total nematode abundance (copies 100 g⁻¹ dry soil) (a) and diversity (b) among different fertilization measures in peach orchards. The same letter indicates no significant differences between the treatments at p > 0.05.

Table2. The abundance of trophic groups of soil nematodes (copies 100 g⁻¹ dry soil) among different fertilization measures (mean ± SE).

Note: CK, control; RMC, rapeseed meal cake fertilization; GM, a green manure Viciavillosa; SF, selenium fertilization; GM + RMC, a measure combined with green manure and rapeseed meal cake fertilizer; SF + RMC, a measure combined selenium fertilizer and rapeseed meal cake fertilizer. Different letters indicate significant differences among fertilization measures based on the LSD’s test (p < 0.05).

The SI index in the SF treatment was significantly higher than in the CK treatment (p < 0.05, Table 3). Additionally, the CI index in both the GM and SF treatments was significantly higher than in the SF + RMC treatment (p < 0.05, Table 3). When comparing the NMF values, the soil bacterivorous nematode groups showed a significant increase in the GM + RMC treatment compared to the SF and SF + RMC treatments (p < 0.05, Table 4). Conversely, the NMF values of soil fungivorous nematode groups were notably higher in the SF treatment compared to the GM and GM + RMC treatments (p < 0.05, Table 4). The total soil nematodes metabolic footprint was significantly elevated in the treatment that combined green manure and organic fertilizer (GM + RMC), as compared to the high-carbon control (CK) and selenium fertilization (SF) treatments (p< 0.05, Figure 2). Furthermore, the metabolic footprint of total soil nematodes was also notably higher in the organic fertilizer treatment (RMC) compared to SF (p< 0.05, Figure 2). However, no significant differences were observed among the six fertilization treatments in the peach orchard for the other nematode indices (Table3), the NMF values of plant-parasitic and, predatory nematodes, as well as the enrichment

Table3. Soil nematode indices under different fertilization measures (mean ± SE).

Note: CK, control; RMC, rapeseed meal cake fertilization; GM, a green manure Viciavillosa; SF, selenium fertilization; GM + RMC, a measure combined with green manure and rapeseed meal cake fertilizer; SF + RMC, a measure combined selenium fertilizer and rapeseed meal cake fertilizer. Different letters indicate significant differences among fertilization measures based on the LSD’s test (p< 0.05).

Table4. Soil nematode metabolic footprints (NMF) values under different fertilization measures (mean ± SE).

Note: CK, control; RMC, rapeseed meal cake fertilization; GM, a green manure Viciavillosa; SF, selenium fertilization; GM + RMC, a measure combined with green manure and rapeseed meal cake fertilizer; SF + RMC, a measure combined selenium fertilizer and rapeseed meal cake fertilizer. Different letters indicate significant differences among fertilization measures based on the LSD’s test (p< 0.05).

Figure2. Total soil nematode metabolic footprint under different fertilization measures.

nematode footprint (Fe) and structural nematode footprint (Fs) (Table 4). Furthermore, the metabolic footprints under the six fertilization measures are concentrated in the region where the enrichment index is less than 50 and the structure index is also less than 50 (i.e., Quadrant D, Figure 3). Only the functional footprint of selenium fertilization treatment (SF) lies in the region where the enrichment index is less than 50 and the functional index is greater than 50 (i.e., Quadrant C, Figure 3).

Figure 3. Metabolic footprint of soil nematodes under different fertilization measures in the peach orchards. A, Quadrant A; B, Quadrant B; C, Quadrant C; D, Quadrant D.

The CCA results showed that the first axis and the second axis explained 15.40% and 7.78% of the community variation at the genus level of soil nematodes, respectively. Among the environmental variables, soil total nitrogen (TN) and total potassium (TK) were the significant factors influencing the community variation of soil nematode genera in the peach orchard (Figure 4(a)). The results of redundancy analysis (RDA) revealed that soil organic carbon, total nitrogen (TN), and total phosphorus (TP), were the most factors influencing the variation of soil nematode functional groups (Figure 4(b)).

Figure4. Relationships between edaphic factors and soil nematodes communities at genus level (a), and individual abundance of each functional group (b). BD, soil bulk density; pH, soil pH value; SOC, soil organic matter; TN, total nitrogen; AP, available phosphorus; AK, available potassium; NH 4 + -N , Ammonium nitrogen; NO 3 − -N , nitrate nitrogen. Ba, bacterivores; Fu, fungivores; He, herbivores; Pr, omnivores-predators. The red arrow represents environmental factors, while the blue arrow stands for the species number of soil nematodes and the abundance of each functional group. The smaller the angle between the two colored arrows is, the greater the mutual influence is.

In our study, a combination of green manure and rapeseed meal cake fertilizer (GM + RMC) significantly increased the total abundance of soil nematodes in the peach orchard (Figure 1(a)), indicating both of green manure and input of organic fertilize (e.g., rapeseed meal cake fertilizer) could significantly increase the number and diversity of nematodes [47][48], through green manure improving soil structure (lower soil bulk density, Table 1) and rapeseed meal cake fertilizer increasing nutrient content [8], such as total nitrogen and available potassium (Table 1). Selenium fertilizer treatment (SF) notably elevated the diversity of soil nematodes compared to other treatments (Figure 1(b)). This result does not support the first hypothesis, which predicted that a combination of two fertilizers would have better effects on soil nematode abundance and diversity than a single fertilizer, indicating that fertilizer measures have complex effects on soil nematode communities. This phenomenon may be caused by two reasons. On one hand, selenium fertilizer can enhance the antioxidant enzyme activity and photosynthesis of crops, thereby promoting their growth [24][30]. On the other hand, we applied selenium through foliar fertilization, which minimizes disturbance and impacts on the soil’s structure and inherent properties. Consequently, this approach may have provided nematodes with a more conducive environment (e.g., higher total potassium content, as shown in Table 1), allowing for greater restoration of their populations over time and space [7].

Notable exceptions were observed in the higher structure index (SI) of the SF treatment compared to the CK (Table 4), indicating minimal disturbance to the soil [49] and the maintenance of soil environment stability [50]. This finding contradicts the first hypothesis, which suggested that a combination of two fertilizers would enhance both the structural index and maturity index. The magnitude of the structure index reflects the connectivity of the soil food web, with higher values indicating higher relative connectivity and longer food chains [51]. The index is primarily influenced by omnivorous/predatory nematodes, which are more sensitive to soil disturbances and require longer recovery times [51]. No significant differences in SI were observed among the other five fertilization treatments (Table 4), implying that restoring nematode community structure and maturity indices in peach orchards may require a prolonged period, potentially influenced by soil organic carbon content [52][53]. Higher levels of soil organic carbon facilitate faster nematode settlement and maturity [52][53]. However, our study found no significant changes in soil organic carbon content across the applied fertilization treatments (Table 1), which explains the lack of profound effects on nematode structural and ecological indices (Table 4). This result may also be attributed to the short duration of fertilization. Therefore, this underscores the importance of long-term monitoring to comprehensively assess the impacts of fertilization on soil properties and nematode communities.

The application of green manure treatment significantly boosted the metabolic activity of bacterivorous nematodes compared to selenium fertilization treatments (namely, SF and SF + RMC treatments). Conversely, selenium fertilization significantly enhanced the metabolic footprint of fungivorous nematodes when compared to green manure measures (specifically, GM and GM + RMC treatments) (Table 3). The stimulatory effect of green manure on the microbial system is primarily attributed to the introduction of fresh organic material or easily assimilable rhizodeposits above- or below-ground [23], which also helps buffer microclimatic conditions [54]. This, in turn, boosts microbial populations and positively influences bacterivorous nematodes [55]. On the other hand, selenium fertilization causes less disturbances to the soil and introduces organic matter into the soil in a way that may promote the fungal community compared to other fertilization measures. This, in turn, exerts a significant bottom-up control over the nematode community within the soil microbial food web, leading to an increase in the abundance of fungivorous nematodes in the soil [51][56].

The functional footprint of nematodes, which reflects the ability of soil food webs to regulate and maintain metabolic balance, is the combined area enclosed by the enrichment footprint and structural footprint [43][57]. Precipitation has been proven to be the main regulator for soil nematode community and footprint among the large gradient of agricultural ecosystems [45]. Our study revealed that the total soil nematodes metabolic footprint was significantly elevated in treatments combining green manure and rapeseed meal cake fertilizer (GM + RMC) and the rapeseed meal cake fertilizer treatment (RMC), compared to the control (CK) and selenium fertilization (SF) treatments, respectively (Figure 2). These changes in nematode community composition and stability ultimately affect the functioning and nutrient cycling of soil ecosystems through biological interactions within the soil food web [58]. The enrichment index (EI) and structural index (SI) can be illustrated on a two-dimensional graph (i.e., faunal analysis), providing an indication of the status of the soil food web [51]. Notably, the metabolic footprints observed under selenium fertilization treatment (SF) fell within Quadrant C (SI > 50, EI < 50) (Figure 3), suggesting a state of undisturbed recycling of endogenous resources indicative of favorable environmental conditions [43][57]. Conversely, metabolic footprints associated with other fertilization treatments predominantly clustered in Quadrant D (SI < 50, EI < 50) (Figure 3), highlighting resource-constrained systems operating under stressful environmental conditions [43][57].

Based on numerous fertilization experiments, a decline in soil pH has been shown to significantly reduce the diversity of soil bacteria and fungi, subsequently impacting the abundance and diversity of soil nematodes [59]. However, in our study, soil pH was not a significant factor influencing soil nematodes abundance (Figure 4), possibly due to the relative minor pH variances (ranging from 0.01 to 0.31) observed among these fertilization treatments (Table 1). Soil organic matter serves as an energy and carbon source for soil organisms, influencing the abundance of soil nematodes [52][53]. For instance, as soil organic matter increases, the overall abundance of soil nematodes and the number of nematodes functional groups also significantly rise [60]. Nevertheless, in our study, soil organic carbon was not a crucial factor affecting soil nematode communities (Figure 4), likely because the convergence of soil organic carbon levels under all the fertilization measures (Table 1) resulted in similar soil nematode community [61]. However, green manure stood out as an exception, significantly reducing soil bulk density compared to other treatments (p < 0.05, Table 1). This underscores the advantages of incorporating green manure into orchard management. Green manure plants, through their root systems growth, promote soil loosening, enhancing porosity, and consequently lower soil bulk density [62]. This improvement in soil structure facilitates better root penetration, water infiltration, and air circulation, all essential for healthy plant growth [63]. Furthermore, our results showed a significantly higher level of available phosphorus (AP, Table 1) in the soil under green manure fertilization. This suggests that planting green manure in orchards does not compete with fruit trees for nutrients but rather contributes to increasing soil AP availability, benefiting fruit tree growth. The decomposition of green manure releases nutrients, including phosphorus, into the soil, enriching it and creating a more conducive environment for plant growth and root development [64]. Our study emphasizes the importance of adopting optimized fertilization practices, particularly the use of green manure, to achieve sustainable agricultural production while safeguarding soil health and promoting ecosystem services. It is worth noting that the effect of organic fertilizer, such as rapeseed meal cake fertilizer treatment (RMC), may not be fully evident within a short duration of just 1.5 years. In the early stages of orchard development, green manure exerts beneficial effects not only through its roots on soil physical properties but also through the favored microclimate created by its coverage of the orchard surface.

Among the environmental variables examined, soil total nitrogen (TN) and total potassium (TK) emerge as significant factors influencing the variability of soil nematode genera in peach orchards (Figure 4(a)). Meanwhile, soil organic carbon (SOC), total nitrogen (TN), and total phosphorus (TP) were identified as the primary drivers influencing the variation of soil nematode functional groups (Figure 4(b)). This underscores the importance of soil nutritional status, particularly the levels of key nutrients like carbon, nitrogen, and phosphorus, in determining the structure and functional diversity of soil nematode communities [65]. Specifically, soil total nitrogen content, which is influenced by fertilization measures, was found to be the most influential factor driving both the soil nematode community and its functional groups. This aligns with our second hypothesis and the research conducted by Zhang et al. [66]. Additionally, these findings emphasize the importance of considering the intricate interplay among multiple environmental factors when studying soil nematode communities and their implications for soil ecosystem functioning and nutrient cycling. Future research could delve deeper into the mechanisms linking soil nematode community changes to ecosystem functions and explore the potential impacts of other environmental variables that may have been overlooked in this study.