Banana (Musa spp.) is a plant native to the hot, humid tropical jungles of Southeast Asia, a geographical area that includes India, Papua New Guinea and the Pacific Islands. Malaysia or Indonesia is being said to be the centre of diversity of these cultures. However, these crops are grown in more than 120 countries in the tropical and subtropical regions of the world. These crops would have been transported from Indonesia to Madagascar then to East Africa, to Zaire and then to West Africa [28] .

Humans migration and the exchange of plant material have allowed the introduction of banana into different ecological zones [29] . In Africa, banana production was introduced by the Arabs and the Portuguese along the east coast of Africa from where they passed through Central Africa countries to West Africa. Desert bananas grown in the highland East Africa are classified as AAA, while cooking bananas (plantain) are AAA, AAB and ABB. These cultivars are widely distributed around the world, particularly in Latin and Central America, the Caribbean, South East Asia and Africa [28] .

Bananas belong to the family of Musaceae, class Liliopsida, and the order Zingiberales. There are perennial plants that reach the height of about 3 meters. Cultivated edible bananas and plantains have evolved from intra- or interspecific crosses between two diploid progenitor species, Musa accuminata (“AA”), and Musa balbisiana (“BB”). However, various important combinations such as AB, AAB, AABB and ABBB also occur as a result of hybridization. M. balbisiana genes are said to induce stronger disease resistance, higher nutritional value with increased starch content. Moreover, they provide hybrids suitable for cooking compared to the pure M. accuminata cultivars mainly suitable for desert use [30] .

Banana and plantain plants are mostly grown in the tropical and subtropical regions, which means they need hot, and humid conditions to thrive well. The growing areas are geographically located at the equator between 20° N latitude and 20° S latitude. However, in subtropics, they are located between 20° and 30° North or South of the equator [31] . The average air temperature required by these crops should be around 30˚C [28] . Moreover, an essential climatic condition that determines where banana and plantain could grow apart temperature is rainfall. Areas that cannot have a well-distributed average annual rainfall of 2000 - 2500 mm require an external source of water supply through irrigation. Banana and plantain need deep, well-drained soils that are very fertile and high in organic matter [30] . Furthermore, they need soils without compaction and excess clay and with a pH between of 4.5 to 8.5. The mineral elements indispensable for the development of banana and plantain are nitrogen, phosphorus, potassium, calcium and magnesium [28] .

The production of banana seedlings in Africa faces the main constraint of unavailability of seedlings in quantity and quality essential to promote the creation of new plantations [2] . Moreover, traditionally a banana plant is obtained from a sucker of another banana plant, and is usually disseminated with the soil of farmland which often contains pathogenic microorganisms. In order to improve the production of banana seedlings, many propagation techniques have been implemented. Currently, propagation of edible plantains and bananas are carried out by micropropagation and macropropagation techniques.

Banana seedlings production can be done by many in vitro techniques, but the most suitable are tissue culture and cell culture leading to the production of vitroplants. Tissue culture is a laboratory multiplication technique. It offers the dual benefit of high propagation rates and elimination of pest and diseases [32] . The technique as applied to banana is also called short tip cultivation or micropropagation due to the small size of the starting material (2 - 20 mm). This technique involves isolating a growing tip from a sucker or male bud and disinfecting it to kill surface organisms. The cleaned tissue is planted in a container with sterile nutrient culture medium. Growth regulators are then added to induce bud proliferation and root development [33] . Growth is achieved under control light, temperature and humidity conditions. Additionally, meticulously clean environment is maintained to keep away microbes that can outgrow and kill propagated plants. The periodic subdivisions and the transfer of the culture to fresh medium lead to exponential multiplication of the tissues. A record of up to 10,000 seedlings from a single sucker in eight months has been reported. Tissue-grown seedlings flower earlier than suckers, give a uniform harvest and have a 20% - 50% advantage in fruit yield [34] . Furthermore, the system has a higher propensity to produce types due to the strong auxins used to induce callus.

Different techniques are employed with regards to the macropropagation method. Prominent among them are split corm and decorticated techniques. The techniques for splitting and decorticating the bulbs are confronted with the problem of contamination by weevils and nematodes. In an attempt to solve this problem and revive banana production, an improved method of macropropagation (PIF technique) was discovered by the African Centre for Research on Banana and plantain, namely CARBAP located in the locality of Njombé in Cameroon, gradually adopted by the farmers and used for the dissemination of new banana and plantain cultivars [35] [36] . The PIF technique is a horticultural propagation method that allows mass production of banana vivoplants in just two to three months in sanitized environment [2] [23] - [26] . Using the PIF technique for vivoplants production in opposition to vitroplants, the following summary steps can be identified ( Figure 1 ).

Step 1: Selection of suckers.

Step 2: Preparation (cleaning) of suckers.

Step 3: Trimming of discard to white.

Step 4: Shelling of suckers.

Step 5: Disinfection of the explants.

Step 6: Drying of the explants in the shade.

Step 7: Incision. If the incision is not well made to destroy all the buds, a single bud will have preference and this action will prevent germination.

Step 8: Introduction and management of the propagator. At the age of two (02) open leaves and three (03) to four rootlets, the seedlings are weaned.

Step 9: Weaning and monitoring under shade. They are further transferred to the shade house for acclimatization and growth.

Banana production can be improved by timely access and use of clean planting material of desirable varieties in adequate quantities. The propagation method has applications at different levels of production. Traditional field grown suckers have the lowest multiplication rates and pre-planting treatment is inadequate to eliminate some pests and diseases. Among improved propagation methods, tissue culture remains superior to others as it enables the elimination of systemic pathogens and allows for faster subsequent multiplication of clean material [37] . Tissue culture plants, however, are still tender and require more care for the first two months after planting. Once established the tissue-cultured plants have a greater ability to produce suckers.

Bananas play a key role in the economies of many developing countries where there are produced. In terms of gross value of production, bananas are the fourth most important food crop in the world after rice, wheat and maize [1] . Bananas are the third tropical fruit crop, with over 145 million tons produced globally in more than 150 countries [3] . As staple food, bananas contribute to the food security of millions of people in much of the developing world and, when traded in local markets, they provide income and employment for the rural population. As an export commodity, they are key contributors to the economy of many low-income food deficit countries, including Cameroon. Most people in the urban communities are notably known for making banana fibre. These fibres are being processed into mats, baskets, ropes, etc., which constitutes a great economic activity. The average world production in tons from 1998 to 2000 was estimated at around 99 million in 2001 [38] . Report indicates that almost 85% of the approximately 145 million tons of world annual harvest of banana and plantain comes from plots and backyard gardens which are mainly situated in the developing world [39] . Plantain banana contains a good amount of daily vitamin C recommended for humans. This vitamin acts as an antioxidant that can boost the immune system. Moreover, it also protects the body against free radical damage associated with ageing, heart diseases, and even certain types of cancer. A high amount of potassium present in plantain bananas is essential for maintaining cellular and body fluids that control the heart rate and blood pressure.

Banana and plantain production are affected by various pests and diseases, including nematodes, weevils, fusarium wilt and Sigatoka diseases [40] . Additionally, we have banana bunchy top virus, steak virus and cucumber mosaic virus [41] . Amongst these diseases ( Table 1 ), the most prominent in bananas is the Sigatoka disease, a fungal disease caused by fungi of the class Ascomycetes and of the genus Mycosphaerella. This disease causes 11% - 80% yield lost in banana plants by reducing the photosynthetic tissues through necrotic leaf lesions [42] . There are two types of banana Sigatoka diseases: yellow Sigatoka and black Sigatoka. Yellow sigatoka disease caused by Mycosphaerella musicola while black Sigatoka disease caused by Mycosphaerella fijiensis is considered one of the most economically important phytosanitary problems [4] .

Table 1. Major diseases affecting banana plants.

1) Origin and distribution

Black Sigatoka disease (BSD) originated from Southeast Asia around Malaysia, the Philippines, Indonesia and Papua New Guinea. It is the main banana disease in banana producing countries, caused by the fungus Mycosphaerella fijiensis. BSD is economically the most destructive leaf disease of banana and plantain worldwide. It induces leaf streaks which significantly reduces the photosynthetically active leaf area. Estimates of losses to black Sigatoka disease for dessert banana and plantains are in the range of 20% - 80% in the absence of fungicides [43] .

2) Symptoms

A first symptom of the disease typically occurs between 7 and 14 days after contamination depending on the local environmental conditions. Following fungal penetration of leaf stomata, colonization of intercellular spaces and the resulting necrotic damage decrease the photosynthetic capacity of the plant, reducing quantity and quality of fruits [44] . Black Sigatoka disease symptoms are recognized in six distinct stages ( Figure 2 ). These stages include:

Stage 1: Faint, minute, reddish-brown specks on the lower surface of the leaf.

Stage 2: Specks elongate, becoming slightly wider to form narrow reddish-brown streaks.

Stage 3: Streaks change color from reddish brown to dark brown or black, sometimes with a purplish tint tinge, clearly visible at the upper surface of the leaf.

Stage 4: The streaks broaden and become more or less fusiform or elliptical in outline, and a water-soaked border appears around each lesion.

Stage 5: The dark brown or black center of each lesion becomes slightly depressed and the water-soaked border becomes more pronounced.

Stage 6: The centers of the lesions dry out turning light grey, with a bright yellow band forming between them and the normal green color of the leaf. Lesions remain visible after leaf necrosis due to their light-colored center and dark border.

3) Control strategies of black Sigatoka disease

The control of black Sigatoka disease is a major challenge for world banana production. Different methods such as cultural control, chemical control, genetic control and biological control have been adopted by farmers to control this disease.

Cultural control generally aims to reduce the level of inoculum and relative humidity in the plantations. To reduce the rate of inoculum, necrotic areas of the leaves or the completely necrotic leaves are excised and then placed on the ground to accelerate their decomposition. The reduction in relative humidity is achieved through an efficient drainage system aimed at preventing water from infiltrating the plots. The higher the fertility, the lower the severity of black Sigatoka. This suggests that good management of organic matter is essential for the sustainable banana production, helping to minimize the severity of black Sigatoka disease [8] . It was confirmed in Cameroon with morphological differences that were observed among fruits of different groups of BSD severity levels. Indeed, fruits with a lower disease severity index (DSI) at flowering had bigger grades than those with high DSI [4] .

Chemical control consists of using pesticides to control and delay the development of M. fijiensis. A pesticide (fungicide, bactericide and nematicide) refers to any synthetic chemical substance used in agriculture to control, destroy, repel or reduce the effect of any living organism harmful to plants. Fungus-specific fungicides can be systemic (benzimidazoles, triazoles, morpholines and strobilurins) or contact (mancozeb or chlorothalonil). The massive and quasi-systemic spraying of fungicides alone or in combination causes epidemics of persistent diseases. However, this method has many disadvantages such as the need for frequent application, the high cost of fungicides, pollution, toxicity and the accumulation of these products [45] .

Generally, the term biological control has been applied to the use of microorganisms and/or natural products extracted or fermented from various sources, and relies to plant bioprotection through some potential bioinsecticidal, biofungicidal, biobactericidal and bionematicidal effects. These natural products are called biopesticides and have been used recently to control black Sigatoka disease caused by Mycosphaerella fijiensis with a significant biofungicidal effect on the resistance of the Big-Ebanga banana-plantain variety in the nursery [2] , as well as with the liquid extract in the field [26] .

Tithonia diversifolia also known as Mexican sunflower is a plant native to Mexico and Central America, but is naturalized in Africa, Australia, Brazil and Asia, where it is considered as an invasive plant or aggressive invader [17] [47] [48] . It grows spontaneously around houses and roads and is considered an annual, biannual or even perennial plant.

Tithonia diversifolia is widely distributed in countries such as India, Cameroon, Kenya, Tanzania, Uganda and Zambia where it was introduced as an ornamental plant. Indeed, Tithonia diversifolia is cultivated and planted in many countries for its beautiful, large flowers (often used for decorations). It is now widely distributed in the tropics, particularly in Africa (West Africa) and Asia where it was speeded on the basis of its multiple uses (therapeutics, fodder plant, green manure, natural insecticide). It is considered today as an invasive plant with a high reproductive capacity, partly thanks to its great capacity to adapt to different ecological zones [49] .

T. diversifolia is a plant found along roadsides and in disturbed areas ( Figure 3 ).

It easily propagated by cuttings or seed, and seed dispersal is by vectors such as humans, livestock and water currents. The seeds of T. diversifolia allow this species to quickly invade disturbed habitats by forming dense stands that prevent the growth of young native plants due to their allelopathic effect [48] . It tolerates heat and drought well and can quickly form large herbaceous bushes.

Tithonia diversifolia is a perennial plant belonging to the kingdom Plantae, the class of Dicotyledonae, the order of Asterales and the family of Asteraceae ( Table 2 ). The genus Tithonia is made up of 11 species with the specific name “diversifolia” means “separated leaves” from the Latin “diversus” [47] .

Tithonia diversifolia is a woody herb or succulent shrub that grows to a height of two to three meters tall in regions of 550 - 1950 meters altitude with mean annual temperature of 15˚C~31˚C and mean annual rainfall of 100 - 200 mm. This plant flowering varies depending on the period, but usually flowers in October and produces about 80,000 to 160,000 seeds annually, with germination rates ranging from 18 to 56% at 25˚C. Its reproduction can be carried out by seeds or more simply by cuttings and layering [47] .

It is having alternate leaves with ten to fifteen centimeters long, blade with three to five lobes. T. diversifolia flower are yellow to orange-yellow and the number of petals variable from eight to fourteen, while the diameters is up to fifteen centimeters. The flowers of the outer ring are sterile, their function being to attract pollinating insects, like the petals of an individual flower. The flowers of the inner disc are hermaphroditic, numerous (one hundred to two hundred), with a tubular corolla of approximately eight millimeters long with five teeth [47] . Its seeds are achenes with four angles, having five millimeters long, topped with a pappus of one hundred to two hundred seeds per flower head. The germination capacity of Tithonia diversifolia is three to five years.

Tithonia diversifolia is full of numerous exploitable virtues, thanks to its composition in a wide range of compounds such as nitrogen (N), potassium (K), phosphorus (P), calcium (Ca), magnesium (Mg), terpenoids, diterpenoids, sesquiterpenoids, flavonoids, phenols, saponins, tannins which gives it biofertilizing and biofungal properties [47] [50] [51] . It has a large quantity of leaves rich in essential nutrients with the particularity of easily decomposing to release them (N, K, P, Ca, Mg) at concentrations that can be higher than those in other agroforestry species ( Table 3 ) and, which not only improves the quality of the soil and at the same time crops production [50] . The decomposition of its leaves constitutes a natural fertilizer for other plants and also a natural amendment for the soil. Furthermore, some studies have highlighted their presence in leaves and stems but also in roots [51] .

In addition, the aqueous and ethanoic extracts of the parts of T. diversifolia are rich in phytochemicals such as alkaloids, flavonoids, phenols, saponins, tannins and terpenoids [52] [53] . However, these phytochemical compounds ( Table 4 ) are more predominant in the leaves followed by the roots and stem; except the phenols which are predominant and distributed in the roots.

In addition, its composition of secondary metabolites such as terpenoids which have antimalarial, antibacterial, antiviral and antifungal pharmacological activities have made T. diversifolia a plant with great exploitable pharmacological potential [54] [55] . Recently, the use of its extracts in fighting cancer disease was efficiently reported [56] . Tithonia diversifolia is a plant that has beneficial effects in various areas, notably in medicine and agriculture. Indeed, since time immemorial, it has been traditionally used to relieve many ailments.

The importance of Tithonia diversifolia in agriculture has been demonstrated in many scientific reports. Indeed, numerous studies have highlighted the application of Tithonia diversifolia during crops cultivation and have proven its effectiveness in organic farming. Due to the ability of its biomass to decompose rapidly and release nitrogen (N), phosphorous (P) and potassium (K) into the soil, it has been extensively used to improve soil [57] . Indeed, fresh biomass of T. diversifolia improves soil fertility and significantly increases soybean yields [57] . Moreover, the shoot biomass of T. diversifolia has been peddled as a potential source of nutrients for lowland rice in Asia and more importantly for maize and vegetables in eastern, southern and central Africa [47] . Its fresh biomass, whether or not combined with inorganic fertilizers, significantly improves the development and yield of cassava [58] . The various uses of T. diversifolia have been shown to be effective in improving the growth and yield of several crops in Africa such as maize [21] [59] , cassava [58] and diverse other crops through its nutrients source potential. Moreover, it has been reported that non-nitrogen-fixing species such as T. diversifolia and Chromolaena odorata can be used for improving soil fertility, furthermore, significantly increasing crop yields from one growing season to the next other [16] .

Similarly, farmers use Tithonia diversifolia as a biopesticide plant in several ways, either to fight against certain pests that attack their crops, or to conserve their crops after harvest.

Tithonia diversifolia is used for bioprotection as a pesticide thanks to its composition of sesquiterpenes, lactones and diterpenoids, which have biological activity against insects and is used as a biocontrol agent notably as biofungicide for crops [60] [61] . Tithonia diversifolia in addition to being a biofertilizer and rich in secondary metabolites (flavonoids, tannins, alkaloids, phenols, and others), has the capacity to induce biostimulating mechanism in the plant though the synthesis of growth promoting macromolecules, as well as defense metabolites such as pathogenesis related (PR) proteins in plants to cope with biotic and abiotic stresses [16] [23] - [27] .

Recent studies carried out in Cameroon on Tithonia diversifolia-based products have shown a stimulating mechanism in banana plant notably their beneficial effects on promoting plantain vivoplants (PIF) growth as well as their biofungicidal effects in vitro and in vivo, especially on M. fijiensis, the fungus responsible for black Sigatoka disease of bananas [2] [16] [23] - [27] . These products obtained from T. diversifolia dried leaves and stems were used alone or combined in the form of flakes, powder and liquid, applied as substrate amendment, mulches or foliar spray. T. diversifolia effect as biofertilizer and biocontrol agent was well highlighted as well as the induction of resistance in vivoplants of plantains treated. Indeed, it was demonstrated that Tithonia diversifolia act through a mechanism of biostimulation of the plantain vivoplants in the nursery and induces a less susceptibility to Mycosphaerella fijiensis through the accumulation before and after inoculation of constitutive and the novo synthesis biomarkers such as proteins, phenols, chitinase, glucanase, peroxidases, polyphenol oxidase compared to the control vivoplants [16] [23] - [27] .

In one hand, the Tithonia diversifolia stimulatory effect has permitted the growth biopromotion and thus to obtain vigorous vivoplants through the improvement of germination rate (pre-emergence and emergence stages) and agromorphological parameters of the vegetative stages such as the length, diameter of the pseudo stems, number of leaves and photosynthetic leaf area. Moreover, the accumulation in treated vivoplants of growth biomarkers such as chlorophylls, amino acids, proteins, phenols and sugars was significantly important [2] [16] [23] - [27] .

In the other hand, the Tithonia diversifolia stimulatory effect has permitted the vivoplants bioprotection through induction of resistance well demonstrated by the less development of necrosis after M. fijiensis inoculation compare to the control ones, as well as the in vitro inhibition of phytopathogens growth in the presence of Tithonia diversifolia-based products. In addition, the accumulation in treated vivoplants of defense biomarkers such as de novo proteins, phenols and defense-related enzymes (phenylalanine ammonia lyase, peroxidase, polyphenol oxidase, chitinase and glucanase) was significantly important. Indeed, the treated vivoplants showed maximum bioprotection against black Sigatoka disease of up to 87% in the nursery compared to controls [16] . However, in the field stage with the mature plantain tree, T. diversifolia liquid extract has shown its bioefficacy with biopesticide potential to reduce the severity of BSD over time, and confirmed by the very low diluted concentration of 25% [26] . T. diversifolia-based products have good growth biopromoting effects, directly on the physiology of plantain vivoplants and indirect on substrate in nursery [27] . Indeed, this organic amendment has modified soil physical properties such as stability of aggregates and porosity that can improve the roots growth, rhizosphere and stimulate plant growth. It shows the potential stimulatory role of T. diversifolia-based products to increases the assimilates availability during growth.

Tithonia diversifolia was also associated with clam shells in the cultivation of banana vivoplants in nurseries and allowed a significant increase of performance in the growth of these plants as well as the induction of less resistance to black Sigatoka disease [2] . Indeed, clam shells are aquatic mollusks of the gastropod class, which consist of calcium carbonate (more than 60%), proteins (5%) and minerals (phosphorus, manganese, zinc, potassium, aluminum, silicon) as well as polysaccharides like chitin [62] . Chitin is a polysaccharide of high molecular weight but low solubility, formed of a chain of N-acetylglucosamines linked together by a glycosidic bond (1 - 4) [64] . Shells in general have shown a biostimulating effect by promoting plant growth and reducing sensitivity to pathogens by highlighting their antifungal properties, growth stimulators and plant defense systems [2] [63] .