<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd">
<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article">
 <front>
  <journal-meta>
   <journal-id journal-id-type="publisher-id">
    jacen
   </journal-id>
   <journal-title-group>
    <journal-title>
     Journal of Agricultural Chemistry and Environment
    </journal-title>
   </journal-title-group>
   <issn pub-type="epub">
    2325-7458
   </issn>
   <issn publication-format="print">
    2325-744X
   </issn>
   <publisher>
    <publisher-name>
     Scientific Research Publishing
    </publisher-name>
   </publisher>
  </journal-meta>
  <article-meta>
   <article-id pub-id-type="doi">
    10.4236/jacen.2024.133020
   </article-id>
   <article-id pub-id-type="publisher-id">
    jacen-135458
   </article-id>
   <article-categories>
    <subj-group subj-group-type="heading">
     <subject>
      Articles
     </subject>
    </subj-group>
    <subj-group subj-group-type="Discipline-v2">
     <subject>
      Chemistry 
     </subject>
     <subject>
       Materials Science, Earth 
     </subject>
     <subject>
       Environmental Sciences
     </subject>
    </subj-group>
   </article-categories>
   <title-group>
    Determination of Metal Content in Soil and Wheat Plant by Inductively Coupled Plasma Mass Spectrometry
   </title-group>
   <contrib-group>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       Iubu (Badea)
      </surname>
      <given-names>
       Anişoara
      </given-names>
     </name> 
     <xref ref-type="aff" rid="aff1"> 
      <sup>1</sup>
     </xref>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       Ion V.
      </surname>
      <given-names>
       Popescu
      </given-names>
     </name> 
     <xref ref-type="aff" rid="aff1"> 
      <sup>1</sup>
     </xref> 
     <xref ref-type="aff" rid="aff2"> 
      <sup>2</sup>
     </xref> 
     <xref ref-type="aff" rid="aff3"> 
      <sup>3</sup>
     </xref>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       Claudia
      </surname>
      <given-names>
       Stihi
      </given-names>
     </name> 
     <xref ref-type="aff" rid="aff2"> 
      <sup>2</sup>
     </xref> 
     <xref ref-type="aff" rid="aff3"> 
      <sup>3</sup>
     </xref>
    </contrib>
    <contrib contrib-type="author" xlink:type="simple">
     <name name-style="western">
      <surname>
       Ioana Daniela
      </surname>
      <given-names>
       Dulama
      </given-names>
     </name> 
     <xref ref-type="aff" rid="aff4"> 
      <sup>4</sup>
     </xref>
    </contrib>
   </contrib-group> 
   <aff id="aff1">
    <addr-line>
     aDoctoral School of Physics, University of Bucharest, Magurele, Romania
    </addr-line> 
   </aff> 
   <aff id="aff2">
    <addr-line>
     aFaculty of Science and Arts, Valahia University of Targoviste, Targoviste, Romania
    </addr-line> 
   </aff> 
   <aff id="aff3">
    <addr-line>
     aAcademy of Romanian Scientists, Bucharest, Romania
    </addr-line> 
   </aff> 
   <aff id="aff4">
    <addr-line>
     aInstitute of Multidisciplinary Research for Science and Technology, Valahia University of Targoviste, Targoviste, Romania
    </addr-line> 
   </aff> 
   <pub-date pub-type="epub">
    <day>
     28
    </day> 
    <month>
     06
    </month>
    <year>
     2024
    </year>
   </pub-date> 
   <volume>
    13
   </volume> 
   <issue>
    03
   </issue>
   <fpage>
    300
   </fpage>
   <lpage>
    311
   </lpage>
   <history>
    <date date-type="received">
     <day>
      21,
     </day>
     <month>
      June
     </month>
     <year>
      2024
     </year>
    </date>
    <date date-type="published">
     <day>
      20,
     </day>
     <month>
      June
     </month>
     <year>
      2024
     </year> 
    </date> 
    <date date-type="accepted">
     <day>
      20,
     </day>
     <month>
      August
     </month>
     <year>
      2024
     </year> 
    </date>
   </history>
   <permissions>
    <copyright-statement>
     © Copyright 2014 by authors and Scientific Research Publishing Inc. 
    </copyright-statement>
    <copyright-year>
     2014
    </copyright-year>
    <license>
     <license-p>
      This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/
     </license-p>
    </license>
   </permissions>
   <abstract>
    This study aims to investigate the level of soil pollution and the grade of accumulation of metals and heavy metals by wheat plants from the soil in different parts of the crop: root, stem, leaf, spike and grain. Sampling campaigns took place in February, April and July when wheat plants were at different growth stages. A number of eight soil samples and eight wheat plant samples were collected. The sampled wheat plant was taken at the same time and from the same place as the soil. Concentrations of Al (aluminium), Cr (chromium), Mn (manganese), Fe (iron), Ni (nickel), Co (cobalt), Cu (copper), Zn (zinc), Sr (strontium), Cd (cadmium) and Pb (lead) were determined by inductively coupled plasma mass spectrometry. Bioconcentration and translocation factors were calculated for the samples analysed.
   </abstract>
   <kwd-group> 
    <kwd>
     Soil
    </kwd> 
    <kwd>
      Wheat Plant
    </kwd> 
    <kwd>
      Heavy Metals
    </kwd> 
    <kwd>
      Translocation Factor
    </kwd>
   </kwd-group>
  </article-meta>
 </front>
 <body>
  <sec id="s1">
   <title>1. Introduction</title>
   <p>
    <xref ref-type="bibr" rid="scirp.135458-"></xref>Soil is the vehicle for plant growth and provides crops with the nutrients needed for vital activities <xref ref-type="bibr" rid="scirp.135458-1">
     [1]
    </xref>.</p>
   <p>
    <xref ref-type="bibr" rid="scirp.135458-"></xref>However, intensive agriculture, unregulated industrial farming, irrational use of fertilizers and urbanization often result in increased plant nutrients, heavy metals and pollutants that cause environmental pollution which affects crop yield and quality <xref ref-type="bibr" rid="scirp.135458-2">
     [2]
    </xref>. Farmers routinely apply excess manure, synthetic fertilizers, and other agrochemicals to improve crop yields without considering the recommended dosages. This can lead to offsite transport and leaching of nutrients, heavy metals, and other pollutants, which can contaminate soils and surface, subsurface, and ground waters <xref ref-type="bibr" rid="scirp.135458-3">
     [3]
    </xref>.</p>
   <p>Heavy metals are a major environmental concern due to their toxicity and detrimental effects on living organisms and ecosystems <xref ref-type="bibr" rid="scirp.135458-4">
     [4]
    </xref>. Heavy metal contamination of soils is one of the main factors contributing to soil quality decline and loss of biodiversity, which is also associated with plant contamination, as metals accumulate in the surface layer of soils and then enter the trophic chain <xref ref-type="bibr" rid="scirp.135458-5">
     [5]
    </xref>. Metals contamination of soils is one of the main factors contributing to soil quality decline and loss of biodiversity as metals accumulate in the surface layer of soils and then enter the trophic chain <xref ref-type="bibr" rid="scirp.135458-6">
     [6]
    </xref>. It is also worth mentioning that metal contamination of soils is rarely evident in the short term, but is characterised by dangerous ecological effects delayed in time <xref ref-type="bibr" rid="scirp.135458-7">
     [7]
    </xref>. Studying the mobility process of metals in soil is one of the most important ways to identify the ability of these contaminants to move between the solid and liquid phases in soil and later into the trophic chain <xref ref-type="bibr" rid="scirp.135458-7">
     [7]
    </xref>. Studying the mobility process of metals in soil is an important way to identify the ability of these contaminants to move between the solid and liquid phases in soil and later into the trophic chain <xref ref-type="bibr" rid="scirp.135458-7">
     [7]
    </xref>. The mobility of metals depends on the chemical form in which they are present in soil, as well as on the physical and chemical properties of the soil (pH, redox condition, electric conductivity, content of the organic matter, granulometric composition) <xref ref-type="bibr" rid="scirp.135458-6">
     [6]
    </xref> <xref ref-type="bibr" rid="scirp.135458-8">
     [8]
    </xref>. The most mobile elements are those of anthropogenic origin occurring in ionic and carbonate form. In contrast, metals associated with silicates and primary minerals have significantly reduced mobility <xref ref-type="bibr" rid="scirp.135458-7">
     [7]
    </xref>. The analysis of the ecological risks associated with metal contamination of soils has received much attention in the literature to date <xref ref-type="bibr" rid="scirp.135458-7">
     [7]
    </xref> <xref ref-type="bibr" rid="scirp.135458-9">
     [9]
    </xref> <xref ref-type="bibr" rid="scirp.135458-10">
     [10]
    </xref>. Soil has a significant impact on human health and quality of life <xref ref-type="bibr" rid="scirp.135458-11">
     [11]
    </xref>. Soil pollution can adversely affect human health.</p>
   <p>Plants have varying abilities to accumulate elements, and their characteristics depend on factors such as plant species, growth stage, and environmental conditions. When we use these types of plants, we will introduce a number of elements into the body that include both essential nutrients and potentially toxic metals <xref ref-type="bibr" rid="scirp.135458-12">
     [12]
    </xref>. Bioconcentration factor is an important parameter in phytoremediation determining information on the metal uptake, its mobilization into the plant tissues, and storage in the shoot parts <xref ref-type="bibr" rid="scirp.135458-13">
     [13]
    </xref>.</p>
   <p>
    <xref ref-type="bibr" rid="scirp.135458-"></xref>Bioconcentration factor (BCF) was calculated using the ratio of metals content in roots (mg∙kg<sup>−</sup><sup>1</sup> dry weight) to its content in the rhizosphere soil (mg∙kg<sup>−1</sup> dry weight) and provides information on the plant species’ ability to uptake and concentrate heavy metals from the soil <xref ref-type="bibr" rid="scirp.135458-14">
     [14]
    </xref>. A value of BCF greater than 1 indicates accumulation in roots biomass, while a BCF lower than 1 indicates no accumulation. The translocation factor (TF) was calculated as the ratio of metal content in leaves (mg∙kg<sup>−1</sup> dry weight) to that in roots (mg∙kg<sup>−1</sup> dry weight). This factor indicates the plant’s capacity to transport heavy metals from roots to leaves, stems or flowers <xref ref-type="bibr" rid="scirp.135458-15">
     [15]
    </xref>. A value of TF greater than 1 indicates that metals are translocated from the roots to the aerial part of the plant <xref ref-type="bibr" rid="scirp.135458-16">
     [16]
    </xref>.</p>
   <p>Cereal product composition has a key role in the human diet. Cereals, especially wheat, may have a high nutritional value for human life, accounting for roughly 1/4 of annual cereal production, wheat supplying 70% of the world’s food. Wheat is a principle food of the human daily die <xref ref-type="bibr" rid="scirp.135458-17">
     [17]
    </xref>. Wheat is an important source of dietary protein and has the potential to be exploited to replace animal protein-based products <xref ref-type="bibr" rid="scirp.135458-18">
     [18]
    </xref>.</p>
   <p>Because wheat is a staple food of the human daily diet <xref ref-type="bibr" rid="scirp.135458-17">
     [17]
    </xref>, this led to the determination of the metal content in wheat plant.</p>
   <p>The locality of Dragomiresti has 68% of the surface is arable soil and it is very important to calculate the content of metals in the soil. The soil and the wheat plant of the study area were used to determine the content of metals in the soil and in different parts of the wheat. The purpose of this study was to: (1) determine the content of heavy metals in farmland soil and wheat; (2) assess the degree of heavy metal pollution in farmland soil; (3) explore the accumulation and migration of metals in wheat.</p>
  </sec><sec id="s2">
   <title>2. Materials and Methods</title>
   <p>The samples analysed in the study come from Geangoești, Dragomirești Jud. Dâmbovița.</p>
   <p>Dragomirești area is located in the south-west of Targoviste Municipality at a distance of 10 km from two old capitals of the Romanian Country, namely Targoviste and Câmpulung, and they are situated at an altitude of 300 meters above sea level.</p>
   <p>The village is crossed from west to east by the Dâmbovița River, and the component villages are on either side of the river. On the right side as the river flows, we have the villages of Decideni, Râncaciov and Ungureni, and on the left Dragomirești, Geangoești and Mogoșești. This locality is located on the border between hills and plains in this area, the relief is mixed, here we find both plain and hilly landforms, but in a smaller proportion.</p>
   <p>In terms of its position to the major landforms, Dragomirești village can be placed on the line separating the hills from the plains, namely Ploiești-Târgoviște-Pitești. The settlement is crossed from east to west by two hills running parallel to the Dâmbovița River flowing between them. At certain points, the hills also form the boundary of the locality, and their heights descend visibly in this geographical setting.</p>
   <p>Sampling was done by actually walking the wheat plot in rows and taking soil and plant samples with a distance of 2 meters between each sample. Soil and plant samples were collected at the same time and from the same place. Sampling was carried out in February and April and wheat sampling in July.</p>
   <p>The metal content of the samples was determined using inductively coupled plasma mass spectrometry (ICP-MS).</p>
   <p>The soil sample (0.4 g) was placed in the digestion vessel, followed by 2.5 mL of 67% high purity nitric acid and 7.5 mL of 37% high purity hydrochloric acid. After shaking (20 min) and digestion (25 min and 1800˚C), the vessels were cooled to room temperature, and then each solution was transferred to volumetric flasks (50 mL).</p>
   <p>The location on the map of the lot from which the samples were collected is shown in <xref ref-type="fig" rid="fig1">
     Figure 1
    </xref>.</p>
   <fig id="fig1" position="float">
    <label>Figure 1</label>
    <caption>
     <title>Figure 1. Location of the lot in Dragomirește (Source: ancpi.geoportal.ro).</title>
    </caption>
    <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2750695-rId13.jpeg?20240823023741" />
   </fig>
  </sec><sec id="s3">
   <title>3. Results and Discussion</title>
   <sec id="s3_1">
    <title>3.1. Determination of Metals in the Crop Soil</title>
    <p>Six soil samples were analysed at the first collection and another six samples at the second collection. The values of the metal concentrations obtained are shown in <xref ref-type="table" rid="tableTables 1-2">
      Tables 1-2
     </xref>. The highest concentration was obtained for aluminium and the lowest was for cadmium</p>
    <table-wrap id="table1">
     <label>
      <xref ref-type="table" rid="table1">
       Table 1
      </xref></label>
     <caption>
      <title>
       <xref ref-type="bibr" rid="scirp.135458-"></xref>Table 1. Descriptive statistics of metals concentration in Geangoesti area (mg/kg). First sampling.</title>
     </caption>
     <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
      <tr> 
       <td class="custom-bottom-td acenter" width="11.40%">Metal<sup>a</sup><sup>)</sup><p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="13.30%">Min<sup>b</sup><sup>)</sup><p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="13.70%">Max<sup>c</sup><sup>)</sup><p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.09%">Mean<sup>d</sup><sup>)</sup><p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.11%">Median<sup>e</sup><sup>)</sup><p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.11%">Std. dev.<sup>f</sup><sup>)</sup><p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="19.29%">RSD<sup>g</sup><sup>)</sup><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-top-td acenter" width="11.40%">Al<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="13.30%">15111.18<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="13.70%">24130.06<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.09%">19335.16<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.11%">18800.42<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.11%">2817.91<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="19.29%">0.074 - 0.106<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Cr<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">43.82<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">61.77<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">51.39<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">51.24<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">5.49<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.072 - 0.106<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Mn<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">905.60<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">1154.12<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">1007.37<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">999.77<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">85.72<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.087 - 0.107<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Fe<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">9078.60<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">11913.95<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">11012.87<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">11279.03<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">922.27<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.069 - 0.101<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Ni<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">379.27<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">475.71<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">419.14<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">416.65<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">33.40<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.078 - 0.107<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Co<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">15.34<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">18.96<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">17.18<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">16.98<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">1.51<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.072 - 0.105<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Cu<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">34.96<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">47.97<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">41.47<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">41.92<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">5.06<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.072 - 0.106<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Zn<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">112.34<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">158.35<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">135.75<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">139.00<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">17.19<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.086 - 0.108<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Sr<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">32.10<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">57.94<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">44.25<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">43.83<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">8.42<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.083 - 0.104<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Cd<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">0.86<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">1.09<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">1.01<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">1.03<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">0.08<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.068 - 0.078<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.40%">Pb<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.30%">23.61<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.70%">32.93<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.09%">29.20<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">29.81<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">3.38<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.29%">0.066 - 0.100<p style="text-align:center"></p></td> 
      </tr> 
     </table>
    </table-wrap>
    <p>where: <sup>a)</sup>metals: Al-aluminiu, Cr-crom, Mn-mangan, Fe-fier, Ni-nichel, Co-cobalt, Cu-cupru, Zn-zinc, Sr-strontiu, Cd-cadmiu, Pb-plumb; <sup>b)</sup>Min: the lowest value obtained; <sup>c)</sup>Max: The highest value obtained; <sup>d)</sup>Mean: is determined by summing a set of numbers and then dividing the total by the count of those numbers; <sup>e)</sup>Median: be defined as the middle number of a group of numbers; <sup>f)</sup>Std. dev: The function estimates the standard deviation based on a sample; <sup>g)</sup>RSD: The Relative Standard Deviation is a measure of the standard deviation of the sample from the sample mean for a given data set.</p>
    <p>Analysing the values in <xref ref-type="table" rid="table1">
      Table 1
     </xref> and <xref ref-type="table" rid="table2">
      Table 2
     </xref>, it can be seen that at the second sampling, the concentration of metals decreases, except for the concentration of aluminium which increases at the second sampling. The second sampling was carried out after the agricultural plot had been fertilised.</p>
    <table-wrap id="table2">
     <label>
      <xref ref-type="table" rid="table2">
       Table 2
      </xref></label>
     <caption>
      <title>
       <xref ref-type="bibr" rid="scirp.135458-"></xref>Table 2. Descriptive statistics of metal concentration in the Geangoesti area (mg/kg). Second sampling.</title>
     </caption>
     <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
      <tr> 
       <td class="custom-bottom-td acenter" width="10.36%">Metal<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="13.41%">Min<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.12%">Max<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.11%">Mean<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.12%">Median<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.12%">Std. dev.<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="19.74%">RSD<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-top-td acenter" width="10.36%">Al<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="13.41%">14842.63<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.12%">27303.48<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.11%">19203.03<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.12%">18644.47<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.12%">3906.04<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="19.74%">0.086 - 0.142<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Cr<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">36.76<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">66.93<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">49.94<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">50.25<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">9.32<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.090 - 0.142<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Mn<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">719.48<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">1164.02<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">974.64<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">1018.77<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">156.96<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.090 - 0.144<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Fe<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">7351.70<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">11383.48<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">9616.60<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">9906.96<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">1430.28<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.088 - 0.133<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Ni<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">291.52<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">485.86<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">400.45<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">417.89<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">64.55<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.090 - 0.143<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Co<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">11.85<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">19.55<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">16.47<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">17.38<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">2.75<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.088 - 0.140<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Cu<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">25.09<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">50.16<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">39.02<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">41.85<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">8.39<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.089 - 0.140<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Zn<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">85.65<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">145.92<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">120.47<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">126.24<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">19.82<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.089 - 0.146<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Sr<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">28.63<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">75.81<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">42.46<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">42.24<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">15.12<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.090 - 0.140<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Cd<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">0.65<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">1.07<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">0.86<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">0.89<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">0.13<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.070 - 0.098<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.36%">Pb<p style="text-align:center"></p></td> 
       <td class="acenter" width="13.41%">16.54<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">32.10<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.11%">24.67<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">25.99<p style="text-align:center"></p></td> 
       <td class="acenter" width="14.12%">5.03<p style="text-align:center"></p></td> 
       <td class="acenter" width="19.74%">0.088 - 0.135<p style="text-align:center"></p></td> 
      </tr> 
     </table>
    </table-wrap>
    <p>To verify the level of heavy metals pollution of the soil, the values obtained by ICP-MS (Inductively coupled plasma mass spectrometry) were compared with the values presented in the ORDER no. 756 of 3 November 1997 <xref ref-type="bibr" rid="scirp.135458-19">
      [19]
     </xref> for the approval of the Regulation on the evaluation of environmental pollution. It was found (<xref ref-type="fig" rid="fig2">
      Figure 2
     </xref>) that the concentration of heavy metals is higher than the normal concentration presented in the order but the concentration of any metal did not exceed the alert threshold for sensitive uses.</p>
    <fig id="fig2" position="float">
     <label>Figure 2</label>
     <caption>
      <title>Figure 2. Heavy metal concentrations in soil (mg/kg).</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2750695-rId14.jpeg?20240823023741" />
    </fig>
    <p>The correlation matrix was performed and the values obtained are presented in <xref ref-type="table" rid="table3">
      Table 3
     </xref>.</p>
    <table-wrap id="table3">
     <label>
      <xref ref-type="table" rid="table3">
       Table 3
      </xref></label>
     <caption>
      <title>
       <xref ref-type="bibr" rid="scirp.135458-"></xref>Table 3. Matrix of correlation coefficients for metals.</title>
     </caption>
     <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
      <tr> 
       <td class="custom-bottom-td acenter" width="5.27%"><p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="8.48%">Al<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="8.66%">Cr<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="9.61%">Mn<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="9.23%">Fe<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="9.40%">Ni<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="8.30%">Co<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="9.04%">Cu<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="9.23%">Zn<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="8.29%">Sr<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="9.22%">Cd<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="4.80%">Pb<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-top-td acenter" width="5.27%">Al<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.48%">1<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.66%"><p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="9.61%"><p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="9.40%"><p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.30%"><p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="9.04%"><p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.29%"><p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="9.22%"><p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Cr<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.9285<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">1<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Mn<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.7633<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">0.9404<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%">1<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Fe<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.1650<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">0.4374<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%">0.6502<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">1<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Ni<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.7132<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">0.9198<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%">0.9751<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.6682<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%">1<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Co<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.5390<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">0.7934<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%">0.9063<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.7341<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%">0.9587<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%">1<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Cu<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.2862<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">0.6124<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%">0.7788<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.7988<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%">0.8646<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%">0.9224<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%">1<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Zn<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.5423<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">0.7584<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%">0.8963<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.7303<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%">0.8932<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%">0.9409<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%">0.7835<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">1<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Sr<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.7507<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">0.8931<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%">0.8557<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.4806<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%">0.9271<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%">0.8753<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%">0.7571<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.7635<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%">1<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%"><p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Cd<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.4468<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">0.6939<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%">0.8604<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.9131<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%">0.8674<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%">0.9154<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%">0.8463<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.9417<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%">0.7108<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%">1<p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%"><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="5.27%">Pb<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.48%">0.2892<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.66%">0.6010<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.61%">0.7962<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.9171<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.40%">0.8486<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.30%">0.9254<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.04%">0.9586<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.23%">0.8630<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.29%">0.6992<p style="text-align:center"></p></td> 
       <td class="acenter" width="9.22%">0.9497<p style="text-align:center"></p></td> 
       <td class="acenter" width="4.80%">1<p style="text-align:center"></p></td> 
      </tr> 
     </table>
    </table-wrap>
    <p>There is a very strong correlation between Cr-Al, Mn-Cr, Ni-Cr, Ni-Mn, Co-Mn, Cd-Fe, Pb-Fe, Co-Ni, Sr-Ni, Cu-Co, Zn-Co, Cd-Co, Pb-Co, Pb-Cu, Cd-Zn, Pb-Cd a strong correlation between Sr-Cr, Zn-Mn, Sr-Mn, Cd-Mn, Cd-Ni, Pb-Ni, Sr-Co, Cd-Cu, Pb-Zn and a moderate correlation between Mn-Al, Sr-Al, Co-Cr, Zn-Cr, Cu-Mn, Pb-Mn, Co-Fe, Cu-Fe, Zn-Fe, Zn-Cu, Sr-Cu, Sr-Zn and Cd-Sr. The lowest correlation coefficient values are obtained for Fe-Al, Cu-Al and Pb-Al.</p>
   </sec>
   <sec id="s3_2">
    <title>3.2. Bioconcentration Factor</title>
    <p>To assess the ability of wheat to accumulate metals from the soil and to translocate them from the root to the aerial part of the plant, the following coefficients were calculated: bioconcentration factor (BCF) and translocation factor (TF).</p>
    <p>If BCF &gt; 1: plant accumulates metals.</p>
    <p>If BCF &lt; 1: the plant does not absorb metals from the soil.</p>
    <p>
     <math xmlns="http://www.w3.org/1998/Math/MathML"> <mrow> 
       <mi>
         B 
       </mi> 
       <mi>
         C 
       </mi> 
       <mi>
         F 
       </mi> 
       <mo>
         = 
       </mo> 
       <mfrac> 
        <mrow> 
         <mi>
           C 
         </mi> 
         <mi>
           R 
         </mi> 
        </mrow> 
        <mrow> 
         <mi>
           G 
         </mi> 
         <mi>
           S 
         </mi> 
        </mrow> 
       </mfrac> 
      </mrow> 
     </math>(1)</p>
    <p>where</p>
    <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
     <tr> 
      <td class="aleft">BCF<p style="text-align:left"></p></td> 
      <td class="aleft">bioconcentration factor<p style="text-align:left"></p></td> 
     </tr> 
     <tr> 
      <td class="aleft">CR<p style="text-align:left"></p></td> 
      <td class="aleft">root metal concentration<p style="text-align:left"></p></td> 
     </tr> 
     <tr> 
      <td class="aleft">GS<p style="text-align:left"></p></td> 
      <td class="aleft">soil metal concentration<p style="text-align:left"></p></td> 
     </tr> 
    </table>
    <p>In February, when the first samples were collected, the plant was 20 - 25 cm tall and had 3 - 4 leaves. As the plant was small, only the soil-plant bioconcentration factor was calculated.</p>
    <table-wrap id="table4">
     <label>
      <xref ref-type="table" rid="table4">
       Table 4
      </xref></label>
     <caption>
      <title>
       <xref ref-type="bibr" rid="scirp.135458-"></xref>Table 4. Soil-plant bioconcentration factor. First sampling.</title>
     </caption>
     <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
      <tr> 
       <td rowspan="2" class="custom-bottom-td custom-top-td acenter" width="10.20%">Sample name<p style="text-align:center"></p></td> 
       <td class="acenter" width="89.80%" colspan="11">Metals<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.12%">Al<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.12%">Cr<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.14%">Mn<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.12%">Fe<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.11%">Ni<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.12%">Co<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.11%">Cu<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.11%">Zn<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.12%">Sr<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.11%">Cd<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.60%">Pb<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-top-td acenter" width="10.20%">G1/S<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.12%">0.154<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.12%">0.230<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.14%">0.406<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.12%">0.119<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.11%">0.139<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.12%">0.499<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.11%">0.682<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.11%">0.152<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.12%">1.243<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.11%">1.043<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.60%">0.564<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.20%">G4/S<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.216<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.288<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.14%">0.492<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.132<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.163<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.409<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.716<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.173<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">1.375<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.786<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.60%">0.341<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.20%">G6/S<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.370<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.448<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.14%">0.605<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.240<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.245<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.665<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.862<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.229<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">1.547<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">1.068<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.60%">0.556<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.20%">G7/S<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.006<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.021<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.14%">0.050<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.004<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.007<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.180<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.103<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.064<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.347<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.542<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.60%">0.270<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.20%">G10/S<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.250<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.300<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.14%">0.444<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.132<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.165<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.543<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.659<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.177<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">1.896<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.985<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.60%">0.631<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.20%">G11/S<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.212<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.214<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.14%">0.478<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.154<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.163<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.310<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.571<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.135<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.920<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.540<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.60%">0.303<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.20%">G13/S<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.079<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.119<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.14%">0.341<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.060<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.088<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.151<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.539<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.179<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">1.287<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.590<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.60%">0.205<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="10.20%">G16/S<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.261<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.343<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.14%">0.517<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.176<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.198<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">0.367<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.787<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.218<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.12%">1.204<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.11%">0.783<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.60%">0.345<p style="text-align:center"></p></td> 
      </tr> 
     </table>
    </table-wrap>
    <p>Analysing the values of metal concentrations in <xref ref-type="table" rid="table4">
      Table 4
     </xref>, it can be seen that sample G1/S (BCF = 1.043) and sample G6/S (BCF = 1.068) accumulate cadmium. Strontium is assimilated by samples G1/S, G4/S, G6/S, G10/S, G13/S end G16/S. For the other metals, the bioconcentration factor value is less than 1, which means that the plant does not accumulate heavy metals.</p>
    <p>The second sampling was done in April, when the plant was developed and the spike was formed. The following were calculated for these samples: root-soil bioconcentration factor and translocation factor in the soil-plant system.</p>
    <p>Soil-root bioconcentration factor was calculated at the second sampling, the results are presented in <xref ref-type="table" rid="table5">
      Table 5
     </xref>.</p>
    <table-wrap id="table5">
     <label>
      <xref ref-type="table" rid="table5">
       Table 5
      </xref></label>
     <caption>
      <title>
       <xref ref-type="bibr" rid="scirp.135458-"></xref>Table 5. Soil-root bioconcentration factor. Second sampling.</title>
     </caption>
     <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
      <tr> 
       <td rowspan="2" class="custom-bottom-td custom-top-td acenter" width="11.57%">Sample name<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="88.43%" colspan="11">Metals<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.02%">Al<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.05%">Cr<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.03%">Mn<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.05%">Fe<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.05%">Ni<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.03%">Co<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.05%">Cu<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.05%">Zn<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.03%">Sr<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.05%">Cd<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.01%">Pb<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-top-td acenter" width="11.57%">R1/S1<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.02%">1.926<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.05%">1.753<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.03%">2.782<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.05%">1.347<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.05%">1.451<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.03%">2.600<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.05%">2.230<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.05%">0.601<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.03%">3.106<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.05%">2.959<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.01%">2.745<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.57%">R4/S4<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.02%">1.143<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.973<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">1.605<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.710<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.751<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">1.686<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.832<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.511<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">2.254<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">3.036<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.01%">1.773<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.57%">R6/S6<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.02%">1.781<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.415<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">2.009<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.074<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.085<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">2.099<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.904<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.465<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">2.428<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">2.323<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.01%">2.102<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.57%">G10/S10<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.02%">1.493<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.242<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">1.761<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.924<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.921<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">1.650<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.327<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.408<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">1.963<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">2.002<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.01%">1.615<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.57%">G11/S11<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.02%">1.568<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.361<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">2.300<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.121<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.215<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">1.938<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.680<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.511<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">2.448<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">2.414<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.01%">1.971<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="acenter" width="11.57%">G13/S13<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.02%">1.143<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.760<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">2.338<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.083<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.260<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">2.055<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">1.462<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">0.511<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.03%">2.842<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.05%">2.233<p style="text-align:center"></p></td> 
       <td class="acenter" width="8.01%">1.873<p style="text-align:center"></p></td> 
      </tr> 
     </table>
    </table-wrap>
    <p>The calculated bioconcentration factor for Al (aluminium), Cr (chromium), Mn (manganese), Fe (iron), Ni (nickel), Co (cobalt), Cu (copper), Sr (strontium), Cd (cadmium), Pb (lead), Zn (zinc) are greater than 1, which means that the plant accumulates the above-named metals.</p>
    <p>The highest value of the bioconcentration factor was recorded for Sr (3.106) and the lowest value for Zn (0.408).</p>
   </sec>
   <sec id="s3_3">
    <title>3.3. Translocation Factor (Tf) of Metals</title>
    <p>Metals concentrations detected in the root and in different parts of the wheat were used to calculate the translocation factor, the translocation factor was calculated as the ratio of the total metal concentration in the stem, leaves, spike and the metal content in the roots.</p>
    <p>
     <math xmlns="http://www.w3.org/1998/Math/MathML"> <mrow> 
       <mtext>
         Tf r-t 
       </mtext> 
       <mo>
         = 
       </mo> 
       <mfrac> 
        <mrow> 
         <mi>
           C 
         </mi> 
         <mi>
           t 
         </mi> 
        </mrow> 
        <mrow> 
         <mi>
           C 
         </mi> 
         <mi>
           r 
         </mi> 
        </mrow> 
       </mfrac> 
      </mrow> 
     </math>(2)</p>
    <p>where</p>
    <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
     <tr> 
      <td class="aleft">Tf<p style="text-align:left"></p></td> 
      <td class="aleft">the root-stem translocation factor<p style="text-align:left"></p></td> 
     </tr> 
     <tr> 
      <td class="aleft">Ct<p style="text-align:left"></p></td> 
      <td class="aleft">the metal concentration in the stem<p style="text-align:left"></p></td> 
     </tr> 
     <tr> 
      <td class="aleft">Cr<p style="text-align:left"></p></td> 
      <td class="aleft">the metal concentration in the root<p style="text-align:left"></p></td> 
     </tr> 
    </table>
    <p>
     <math xmlns="http://www.w3.org/1998/Math/MathML"> <mrow> 
       <mtext>
         Tf t-f 
       </mtext> 
       <mo>
         = 
       </mo> 
       <mfrac> 
        <mrow> 
         <mi>
           C 
         </mi> 
         <mi>
           f 
         </mi> 
        </mrow> 
        <mrow> 
         <mi>
           C 
         </mi> 
         <mi>
           t 
         </mi> 
        </mrow> 
       </mfrac> 
      </mrow> 
     </math>(3)</p>
    <p>where</p>
    <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
     <tr> 
      <td class="aleft">Tf t-f <p style="text-align:left"></p></td> 
      <td class="aleft">the stem-to-root translocation factor<p style="text-align:left"></p></td> 
     </tr> 
     <tr> 
      <td class="aleft">Cf<p style="text-align:left"></p></td> 
      <td class="aleft">the metal concentration in leaves<p style="text-align:left"></p></td> 
     </tr> 
     <tr> 
      <td class="aleft">Cr<p style="text-align:left"></p></td> 
      <td class="aleft">the metal concentration in the stem<p style="text-align:left"></p></td> 
     </tr> 
    </table>
    <p>
     <xref ref-type="bibr" rid="scirp.135458-"></xref> 
     <math xmlns="http://www.w3.org/1998/Math/MathML"> <mrow> 
       <mtext>
         Tf t-s 
       </mtext> 
       <mo>
         = 
       </mo> 
       <mfrac> 
        <mrow> 
         <mi>
           C 
         </mi> 
         <mi>
           s 
         </mi> 
        </mrow> 
        <mrow> 
         <mi>
           C 
         </mi> 
         <mi>
           t 
         </mi> 
        </mrow> 
       </mfrac> 
      </mrow> 
     </math>(4)</p>
    <p>where</p>
    <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
     <tr> 
      <td class="aleft">Tf t-s<p style="text-align:left"></p></td> 
      <td class="aleft">the stem-spike translocation factor<p style="text-align:left"></p></td> 
     </tr> 
     <tr> 
      <td class="aleft">Cs<p style="text-align:left"></p></td> 
      <td class="aleft">the metal concentration in the spike<p style="text-align:left"></p></td> 
     </tr> 
     <tr> 
      <td class="aleft">Ct<p style="text-align:left"></p></td> 
      <td class="aleft">the metal concentration in the stem<p style="text-align:left"></p></td> 
     </tr> 
    </table>
    <p>From <xref ref-type="fig" rid="fig3">
      Figure 3
     </xref> it can be noticed that the plant does not translocate metals to the stem, the TF value is less than 1. The highest TF values were recorded for Sr and Cd.</p>
    <fig id="fig3" position="float">
     <label>Figure 3</label>
     <caption>
      <title>Where G1T/R; G4T/R; G6T/R; G7T/R; G10T/R; G11T/R; G13T/R is the name of the samplesFigure 3. Stem-to-root translocation factor.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2750695-rId23.jpeg?20240823023742" />
    </fig>
    <fig id="fig4" position="float">
     <label>Figure 4</label>
     <caption>
      <title>Where G1 F/T; G4 F/T; G6 F/T; G7 F/T; G10 F/T; G11 F/T; G13 F/T; G16 F/T is the name of the samplesFigure 4. Strain-flaxen translocation factor.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2750695-rId24.jpeg?20240823023742" />
    </fig>
    <p>
     <xref ref-type="fig" rid="fig4">
      Figure 4
     </xref> shows that the plant translocates aluminum, chromium, manganese, iron, nickel, cobalt, copper, zinc, strontium, cadmium and lead from stem to leaf. The calculated transfer factor values indicate easier translocation for aluminium, chromium, iron and nickel and heavier for cobalt, copper, zinc strontium cadmium and lead.</p>
    <p>
     <xref ref-type="fig" rid="fig5">
      Figure 5
     </xref> shows that the metals aluminum, iron, nickel and strontium are not translocated to the spike.</p>
    <p>While chromium is translocated only by sample G13S/T; manganese is translocated by sample G13S/T; zinc is translocated by samples G1 S/T, G7 S/T, G13S/T; cadmium is translocated by sample G1 S/T and lead is translocated by sample G1 S/T.</p>
    <fig id="fig5" position="float">
     <label>Figure 5</label>
     <caption>
      <title>Where G1 S/T; G7 S/T; G13 S/T; G16 S/T is the name of the samples.Figure 5. Strain-spike translocation factor.</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2750695-rId25.jpeg?20240823023742" />
    </fig>
   </sec>
   <sec id="s3_4">
    <title>3.4. Evaluation of Metals in Wheat Grains</title>
    <p>
     <xref ref-type="fig" rid="fig6">
      Figure 6
     </xref> shows the concentrations of metals found in wheat grains. The order of the values of the metal concentrations in the grain wheat is as follows: Mn &gt; Al &gt; Pb &gt; Fe &gt; Zn &gt; Cu &gt; Sr &gt; Ni &gt; Co &gt; Cr &gt; Cd.</p>
    <fig id="fig6" position="float">
     <label>Figure 6</label>
     <caption>
      <title>Figure 6. Metals in wheat grains (mg/kg).</title>
     </caption>
     <graphic mimetype="image" position="float" xlink:type="simple" xlink:href="https://html.scirp.org/file/2750695-rId26.jpeg?20240823023742" />
    </fig>
    <table-wrap id="table6">
     <label>
      <xref ref-type="table" rid="table6">
       Table 6
      </xref></label>
     <caption>
      <title>
       <xref ref-type="bibr" rid="scirp.135458-"></xref>Table 6. Arsenic and heavy metals in wheat grains.</title>
     </caption>
     <table class="MsoTableGrid custom-table" border="0" cellspacing="0" cellpadding="0"> 
      <tr> 
       <td rowspan="2" class="custom-top-td acenter" width="29.38%">mg/kg<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="70.62%" colspan="6">Metals<sup>a</sup><sup>)</sup><p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-bottom-td custom-top-td acenter" width="9.53%">Cu<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="8.37%">Zn<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="9.23%">Pb<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="14.31%">As<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="14.50%">Cd<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td custom-top-td acenter" width="14.68%">Hg<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-top-td acenter" width="29.38%">sample of the wheat<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="9.53%">34.400<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="8.37%">38.483<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="9.23%">83.159<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.31%">not detected<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.50%">not detected<p style="text-align:center"></p></td> 
       <td class="custom-top-td acenter" width="14.68%">not detected<p style="text-align:center"></p></td> 
      </tr> 
      <tr> 
       <td class="custom-bottom-td acenter" width="29.38%">Order No 975<p style="text-align:center"></p>Maximum limits for arsenic and heavy metals in wheat<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="9.53%">5<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="8.37%">15<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="9.23%">1<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.31%">0.2<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.50%">0.2<p style="text-align:center"></p></td> 
       <td class="custom-bottom-td acenter" width="14.68%">0.05<p style="text-align:center"></p></td> 
      </tr> 
     </table>
    </table-wrap>
    <p><sup>a</sup><sup>)</sup>Cu-copper, Zn-zinc, Pb-lead, As-arsen, Cd-cadmium, Hg-mercury.</p>
    <p>Analysing the values in <xref ref-type="table" rid="table6">
      Table 6
     </xref> it can be seen that the concentration of Cu, Zn and Pb is higher than the maximum limit presented in Order No 975. The metals As (arsenic), Cd (cadmium), Hg (mercury) were not detected in the analysed wheat sample.</p>
   </sec>
  </sec><sec id="s4">
   <title>4. Conclusion</title>
   <p>Analysis of the 16 soil samples showed that the heavy metal concentrations did not exceed the alert threshold for the sensitive use type. The calculated transfer coefficients for the metals aluminium, chromium, manganese, iron, nickel, cobalt, copper, strontium, cadmium, lead, and zinc are greater than 1, which means that the plant accumulates these metals. The highest value of the bioconcentration factor was recorded for Sr (3.106) and the lowest value for Zn (0.408). Calculating the stem-root translocation factor, it was observed that its value is less than 1, which means that the plant does not translocate metals to the stem. The plant translocates aluminum, chromium, manganese, iron, nickel, cobalt, copper, zinc, strontium, cadmium and lead from stem to leaf. The calculated transfer factor values indicate easier translocation for aluminum, chromium, iron and nickel and harder for cobalt, copper, zinc strontium cadmium and lead. The metals aluminum, iron, nickel and strontium were found not to translocate to the spic. Eleven metals were detected in wheat grains. The order of metal concentrations is as follows: Mn &gt; Al &gt; Pb &gt; Fe &gt; Zn &gt; Cu &gt; Sr &gt; Ni &gt; Co &gt; Cr &gt; Cd. In the wheat flour obtained from the analyzed batch, it was found that the concentration of Cu, Zn and Pb exceeded the maximum permissible limit.</p>
  </sec>
 </body><back>
  <ref-list>
   <title>References</title>
   <ref id="scirp.135458-ref1">
    <label>1</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Arce-López, B., Coton, M., Coton, E. and Hymery, N. (2024) Occurrence of the Two Major Regulated Mycotoxins, Ochratoxin A and Fumonisin B1, in Cereal and Cereal-Based Products in Europe and Toxicological Effects: A Review. Environmental Toxicology and Pharmacology, 109, Article 104489. &gt;https://doi.org/10.1016/j.etap.2024.104489 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref2">
    <label>2</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Ruas, R.d.B., Costa, L.M.S. and Bered, F. (2022) Urbanization Driving Changes in Plant Species and Communities—A Global View. Global Ecology and Conservation, 38, e02243. &gt;https://doi.org/10.1016/j.gecco.2022.e02243 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref3">
    <label>3</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Jaja, N., Codling, E.E., Timlin, D., Rutto, L.K. and Reddy, V.R. (2023) Phytoremediation Efficacy of Native Vegetation for Nutrients and Heavy Metals on Soils Amended with Poultry Litter and Fertilizer. International Journal of Phytoremediation, 25, 1423-1434. &gt;https://doi.org/10.1080/15226514.2022.2161466 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref4">
    <label>4</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Abd Elnabi, M.K., Elkaliny, N.E., Elyazied, M.M., Azab, S.H., Elkhalifa, S.A., Elmasry, S., et al. (2023) Toxicity of Heavy Metals and Recent Advances in Their Removal: A Review. Toxics, 11, Article 580. &gt;https://doi.org/10.3390/toxics11070580 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref5">
    <label>5</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Wieczorek, J., Baran, A. and Bubak, A. (2023) Mobility, Bioaccumulation in Plants, and Risk Assessment of Metals in Soils. Science of the Total Environment, 882, Article 163574. &gt;https://doi.org/10.1016/j.scitotenv.2023.163574 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref6">
    <label>6</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Li, Q., Wang, Y., Li, Y., Li, L., Tang, M., Hu, W., et al. (2022) Speciation of Heavy Metals in Soils and Their Immobilization at Micro-Scale Interfaces among Diverse Soil Components. Science of the Total Environment, 825, Article 153862. &gt;https://doi.org/10.1016/j.scitotenv.2022.153862 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref7">
    <label>7</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Wang, C., Zhang, Q., Kang, S., Li, M., Zhang, M., Xu, W., et al. (2023) Heavy Metal(loid)s in Agricultural Soil from Main Grain Production Regions of China: Bioaccessibility and Health Risks to Humans. Science of the Total Environment, 858, Article 159819. &gt;https://doi.org/10.1016/j.scitotenv.2022.159819 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref8">
    <label>8</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Kicińska, A., Pomykała, R. and Izquierdo-Diaz, M. (2021) Changes in Soil pH and Mobility of Heavy Metals in Contaminated Soils. European Journal of Soil Science, 73, 1-14. &gt;https://doi.org/10.1111/ejss.13203 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref9">
    <label>9</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Wieczorek, J. and Baran, A. (2021) Pollution Indices and Biotests as Useful Tools for the Evaluation of the Degree of Soil Contamination by Trace Elements. Journal of Soils and Sediments, 22, 559-576. &gt;https://doi.org/10.1007/s11368-021-03091-x 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref10">
    <label>10</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Kijowska-Strugała, M., Agnieszka, B., Magdalena, S.-B., Łukasz, W. and Paweł, P. (2022) Soil Quality under Different Agricultural Land Uses as Evaluated by Chemical, Geochemical and Ecological Indicators in Mountains with High Rainfall (Darjeeling Himalayas, India). Journal of Soils and Sediments, 22, 3041-3058.
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref11">
    <label>11</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Tran, H.M., Tsai, F., Lee, Y., Chang, J., Chang, L., Chang, T., et al. (2023) The Impact of Air Pollution on Respiratory Diseases in an Era of Climate Change: A Review of the Current Evidence. Science of the Total Environment, 898, Article 166340. &gt;https://doi.org/10.1016/j.scitotenv.2023.166340 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref12">
    <label>12</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Ngoc Ba, V., Thien, B.N., Phuong, H.T., Thi Hong Loan, T. and Anh, T.T. (2024) Bioconcentration and Translocation of Elements from Soil to Vegetables and Associated Health Risk. Journal of Food Composition and Analysis, 132, Article 106296. &gt;https://doi.org/10.1016/j.jfca.2024.106296 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref13">
    <label>13</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Sharma, P., Singh, S.P. and Tong, Y.W. (2022) Phytoremediation of Metals: Bioconcentration and Translocation Factors. In: Sharma, P., Pandey, A., et al., Eds., Current Developments in Biotechnology and Bioengineering, Elsevier, 19-37. &gt;https://doi.org/10.1016/b978-0-323-99907-6.00002-5 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref14">
    <label>14</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Forsyth, K., Dia, A., Marques, R., Prudêncio, M.I., Diamantino, C., Carvalho, E., et al. (2023) Bioconcentration and Translocation of Rare Earth Elements in Plants Collected from Three Legacy Mine Sites in Portugal. Frontiers in Environmental Science, 11, 1-18. &gt;https://doi.org/10.3389/fenvs.2023.1191909 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref15">
    <label>15</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Maiti, K.S., Dipita, G. and Deep, R. (2022) Phytoremediation of Fly Ash: Bioaccumulation and Translocation of Metals in Natural Colonizing Vegetation on Fly Ash Lagoons. Handbook of Fly Ash, 2022, 501-523.
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref16">
    <label>16</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Chitimus, D., Nedeff, V., Mosnegutu, E., Barsan, N., Irimia, O. and Nedeff, F. (2023) Studies on the Accumulation, Translocation, and Enrichment Capacity of Soils and the Plant Species Phragmites Australis (Common Reed) with Heavy Metals. Sustainability, 15, Article 8729. &gt;https://doi.org/10.3390/su15118729 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref17">
    <label>17</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Mohadeseh, P., Nabi, S., Mahmoud, B. and Aliasghar, M. (2022) Heavy Metals in Wheat Grain and Its Impact on Human Health: A Mini-Review. Journal of Chemical Health Risks, 12, 421-426. &gt;https://jchr.org/index.php/JCHR/article/view/298 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref18">
    <label>18</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     Shewry, P.R. (2024) Can We Increase the Use of Wheat and Other Cereals as Sources of Protein? Journal of Cereal Science, 117, Article 103899. &gt;https://doi.org/10.1016/j.jcs.2024.103899 
    </mixed-citation>
   </ref>
   <ref id="scirp.135458-ref19">
    <label>19</label>
    <mixed-citation publication-type="other" xlink:type="simple">
     (2011) ORDIN nr. 756 din 3 noiembrie 1997. &gt;https://legislatie.just.ro/Public/DetaliiDocument/13572
    </mixed-citation>
   </ref>
  </ref-list>
 </back>
</article>