From this study, none of the recorded anti-insect plant species identified is useable against lice species. However, the population of our study area recognizes the existence of this type of bloodsucking insect and its associated diseases.

Epidemic typhus is known as a severe acute disease with high mortality if not treated. Wartime would be the most catastrophic moment regarding lice-borne pathogens and diseases, as was the case for Burundi during the civil war of 1993 - 2002. In 1997, Burundi faced the largest epidemic of typhus associated with trench fever after its resurgence in 1995 and 12 years of absence in the country [41] . This disease, whose pathogen agent (bacteria of the Rickettsia genus) was transmitted to humans by Pediculus humanus corporis [42] , affected more than a hundred people in the country, particularly in 5 provinces, including Kayanza and Muranvya that are concerned in this study [43] . Russia alone recorded more than 25 million cases and more than three million deaths during the Revolution. Different regions, including North Africa, southern Italy, and Eastern and Central Europe, were also affected during and after World War I and II [44] . Sporadic cases were later observed across the world (in North Africa, North America, Russia, China, etc.) and, recently, lice-borne Rickettsia prowazekii exhibited a minimum prevalence of 7.3%, a maximum prevalence of 21.2% and an overall prevalence of 5.57% in refugee camps and detention centers in the east of 176 Africa, Burundi and Rwanda [44] [45] .

Our respondents recognized the relationship between the lack of hygienic conditions and the spread of lice-borne pathogens and diseases. They confirmed that improving hygiene conditions, i.e., shaving hair and heating clothes with hot water or removing clothing invaded by lice, effectively helps control lice and prevent lice-associated diseases. Such hygienic measures would not only be effective against typhus but also against other lice-borne diseases such as relapsing fever, trench fever, and plague [12] [41] [44] .

Nicotiana tabacum was the only reported plant species with anti-bedbug properties. However, its use for insecticidal purposes remains limited to isolated cases particularly due to ignorance or adoption of pesticides. This justifies the related low consensus between respondents (ICs = 0.02). Despite the diversity of this arthropod group, no distinction in specific sensitivity was reported by respondents.

Bedbugs remain of great public health and socioeconomic concern. The genus of Cimex comprises different species (i.e., C. lectularius, C. hemipterus, C. columbarius, C. pipistrelli, C. dissimilis, and Oeciacus hirundinis) that most feed on humans and animals, including domestic animals, birds, and bats. Their bites are generally associated with cutaneous and allergic reactions (ex. their allergens can trigger asthmatic reactions [41] ), anemia, stress, anxiety, sleep disturbance, secondary infections, and mental health impacts. Cimex lectularius and Cimex hemipterus have recently become a common societal pest [41] and have given rise to high clinical interest [1] [12] . From the majority of our respondents (more than 65%) and the literature [41] , bedbugs are typically prevalent in the most densely populated or frequented places (school dormitories, homes, hotels, detention centers, health care environments, etc.).

Bedbugs population is rapidly increasing (100 - 500% increase annually) [46] ) and spreading worldwide [41] [47] , while fighting against them is becoming financially and ecologically more challenging [12] . People generally resort to conventional methods to clean up infested places, objects, or multiplication sites (clothing, mattresses, sheets, etc.). However, using pesticides in schools may lead to acute illness among school employees and students, with an incidence rate of 35% to 65% [12] [48] . It was also estimated that the cost of disinfecting a house using standard insecticides and replacing infested belongings was estimated at nearly $2500 - $3000 (USD) per infestation [47] , whereas dealing with cleaning invasions of commercial and industrial workplaces may cost millions of dollars [49] . Very few Burundians, particularly those in the study area, would be able to afford these costs.

Thus, to cope with bedbugs, it is mandatory to set up a fully integrative approach [12] , taking into account cost-effectiveness, health, and ecological efficiency. In this regard, the application of Nicotiana tabacum, as a natural alternative to synthetized chemicals meets all of these expectations. Despite being non-edible, N. tabacum economic importance makes it one of the most extensively cultivated plants worldwide mainly for the cigarette industry and for human consumption needs. Due to its adaptability to both tropical and temperate conditions, Burundi offers a well-indicated cultivation terrain.

Besides its nicotine, the principal chemical components, N. tabacum contains in its leaves, stems, roots, and floors more than 4000 chemicals or secondary metabolites compounds, including phenolic compounds, nicotphenols A - C [50] , cembranoids, flavonoids alkaloids, and terpenoids [51] . The bioactivity of N. tabacum compounds leads it to be efficiently used against various parasites, bacteria (i.e., Escherchia coli [52] , Klebsiella pneumonia, Listeria monocytogenes, Viridans streptococci, Mycobacterium phlei [53] , Mycobacterium tuberculosis [54] , Staphylococcus aureus [52] [55] ), virus [51] [55] [56] , HIV (Human Immunodeficiency Virus) [50] [56] , Cryptococcus neoformans [57] , Streptococcus pyogenes [57] , Bacillus subtilis [52] , fungi such as Candida albicans [53] [55] [57] , etc. Research continues to prove the bioactivity of N. tabacum components against many bedbugs associated pests or pathogens [12] , including Bartonella quintana, Burkholderia multivorans, Trypanosoma cruzi, Rickettsia parkeri, hepatitis B virus, hepatitis C virus, methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus, Penicillium chrysogenum, Stenotrophomonas maltophilia, Enterobacter hormaechei, Bacillus licheniformis, and Staphylococcus saprophyticus [46] [47] [58] , etc. The latter may be responsible for more than 40 human communicable diseases, although no evidence has proved their transmission by bedbugs [12] [58] [59] , even for the viruses that they may harbor, such as hepatitis B virus [60] and HIV [1] .

It should also be noted that the use of N. tabacum is extended to managing many other human and veterinary ailments such as ulcers, respiratory tract infections, cancer, cough, snake bite [61] , rheumatic swelling, skin diseases, painful piles, and stings [62] . It is also used against termites [51] [55] [56] and as a vermifuge [61] [63] [64] [65] .

Based on these examples, it is clear that N. tabacum presents various tangible opportunities. Therefore, it deserves to be valued, developed, and preserved. However, many challenges need to be considered and addressed. N. tabacum cultivation development is influenced by several factors, including soil conditions (fertility and physicochemical properties), environmental conditions, cultural practices, economic and health considerations, and farming environmental impact.

Compared to other crops, tobacco is extremely demanding in terms of growing space and essential soil nutrients (nitrogen, phosphorus, potassium, magnesium, calcium, etc.), fertilizers, agrochemicals, and growth regulators [66] [67] . This is likely to lead to the quick loss of soil fertility [68] , soil erosion, and land degradation, deforestation (to find new appropriate soil) than many other crops.

In Burundi, tobacco cultivation is declining in favor of other crops, less nutrient demanding, more economically profitable, sources of food for humans and domestic animals, and less or not dangerous to health and the environment. According to our survey, the scarcity of arable land and the increasing lack of fertilizers are the main factors. Furthermore, awareness of the health impact of smoking and relative prevention measures, especially by avoiding smoking, has reduced the number of people interested in this crop. Among the people interviewed, no one thinks of making this plant a crop of choice for family economic development. Furthermore, apart from its use in cigarettes and as an anti-bedbug, more than 80% of our respondents were unaware of the other benefits of N. tabacum, including its antimicrobial role.

Four plant species, namely Eucalyptus globules subsp. maidenii, Cedrela odorata, Cymbopogon citratus, and Tetradenia riparia, are cited as mosquito repellent.

a. Eucalyptus globulus sub sp. Maidenii

Eucalyptus globulus subsp. Maidenii is recognized as the most appropriate mosquito repellent and the most abundant in the study area. Of the Mosquitoes genera, Anopheles, Aedes, and Culex are the most involved in transferring pathogens responsible for various diseases, including malaria, dengue fever, lymphatic filariasis, chikungunya, Zika virus disease, West Nile fever, yellow fever, and Japanese encephalitis [10] . In Burundi, malaria, caused by Plasmodium species, remains the most prevalent disease and the leading cause of mortality and morbidity [69] [70] [71] , followed by diarrheal diseases, acute respiratory infections (ARI), malnutrition, and AIDS [7] . Malaria, plasmodium, and mosquitoes are all endemic across the whole country. In 2009, malaria was responsible for more than 48% of deaths in children under five years of age and more than 50% of reasons for consultation in hospitals and health centers in the country. The malaria epidemic raged in 2019, reaching 843,000 cases per million inhabitants [69] and more than 2.600 deaths, whereas 8,571,897 malaria cases and 3170 deaths were recorded in early 2020 [72] against 5,957 deaths in 2021 [73] .

Essential oil from Eucalyptus globulus subsp. maidenii exhibits strong mosquito-killing and repellent activity [74] which may be due to the phenolic acids released by its leaves. Eucalyptus globulus essential oil contains in high concentration 1,8-cineole, a substance that is also contained in the leaves of Salvia and Artemisia, and is endowed with several healing properties [75] . However, it was reported that Eucalyptus citriodora would be most effective [76] against mosquitoes and flies.

Apart from the anti-mosquitoes activity, Eucalyptus globulus extracts, particularly its essential oil have a broad spectrum of biological activity that can attract much scientific, medicinal, pharmacological, and industrial interest. As shown hereafter, they include anti-bacterial, anti-fungal, insecticidal/insect repellent, anti-nematodes, herbicidal, anti-mites, and acaricidal [76] .

- Antibacterial activity (i.e., bioactivity against Listeria monocytogenes, Bacilus subtilis, Staphylococcus aureus, Enterococcus spp, Shigella flexneri, Klebsiella pneumoniae, Salmonella choleraesuis, Salmonella enteritidis, Proteus mirabilis, Xanthomonas vesicatoria, Enterobacter aerogenes. Such an antibacterial property makes 1, 8-cineole capable of healing wounds and increasing the permeability of the skin. It is also effective in treating blisters, burns, cuts, and sores [77] . However, it may not be active on some other microorganisms, including Escherichia Coli or Pseudomonas aeruginosa [78] .

- Antifungal activity: Essential oil from Eucalyptus globulus is capable of inhibiting the activity of various fungi species such as Aspergillus flavus [79] , Aspergillus parasiticus [79] [80] , Alternaria solani, Fusarium oxysporum f.sp. niverum, Usarium graminearum, Rhizoctonia solani, and Venturia pirina, all of them exhibiting the inhibiting activity of globulol [81] , Fusarium solani, Sclerotinia sclerotiorum, Rhizoctonia solani [82] , Fusarium moniliforme [80] , Trichophyton mentagrophytes, Microsporum gypsum (filamentous forms) [83] , and Candida albicans [83] [84] [85] (the three last ones exhibiting more activity of Eucalyptus citriodora oil than E. globulus), Saccharomyces cerevisiae [84] [85] , Candida parapsilosis [85] , etc. Owing to this antifungal activity, together with anti-bacterial, anti-insect, and anti-oxidant properties, extracts and essential oil from Eucalyptus globulus constitute potential natural food preservatives, a valuable component for food stock management. To support the safety of such use, eucalyptus oil is on the list of Generally Regarded as Safe established by the Food and Drug Authority of USA and classified as non-toxic; it was approved as a flavoring agent in foods, candies, and confectionery items. At low concentrations, it is extensively used in soaps, detergents, and perfumes [76] .

- Anti-inflammatory and analgesic effects: Due to its 1,8-cineole content, essential oil from E. globulus exerts an anti-inflammatory and analgesic activity [86] associated with anti-oxidant properties [75] [87] . It may inhibit the formation of prostaglandins and cytokines by monocytes, hence the anti-inflammatory effect, and thus, can be used as a painkiller, particularly in muscular pain and rheumatic diseases [75] .

- Anti-cancer and hypoglycemic effect: Ongoing researches have not yet proven tangible results.

- Anti-cancer and hypoglycemic effect: Ongoing research has not yet proven tangible results.

- Antiviral, expectorant, and mucolytic activity (direct stimulation of the secretory cells of the bronchial mucosa, helps thin bronchial secretions to expel easily, relaxing the smooth muscles of the airways) [88] . Eucalyptus essential oil exerts spasmolytic effects in the attenuation of inflammatory responses caused by viruses, in particular respiratory diseases [89] .

It is used to treat headaches, sinusitis, asthma, sore throats, nerve pain, skin infections, urinary tract infections, ear infections, bronchitis [90] , and Herpes Simplex Virus (HSV1 and HSV2) [91] [92] . Using eucalyptus essential oils is one of the alternative antiviral solutions to the absence of an adequate therapeutic remedy for treating human infectious diseases of viral origin, such as influenza, SARS-COV-2, and recently COVID-19. These essential oils serve as complements for the treatment of symptoms or to exert effects on possible pharmacological targets of viruses [89] .

In Burundi, eucalyptus has long been used in the form of drinkable solutions or inhaled vapors to relieve respiratory problems, chest pain, headaches and the flu. The recent COVID-19 pandemic has caused a widespread rush to use eucalyptus extract to combat this pandemic. However, since it is combined with other solutions, including pharmaceutical products, it becomes difficult to estimate its effectiveness level. Other uses include being a source of notable essential oils, distillates, tannins, nectar, pollen, shade, honey flora, etc.

The first Eucalyptus plantations began in Burundi in 1931 [93] [94] . Since then, it has been widely adopted and cultivated. The planted areas increased continuously, reaching 18.000 ha in 1973, 25.000 ha in 1976 [93] , and more in the following years. Its expansion across the country results from many reasons. The first reasons are the higher wood and charcoal quality and its multiple uses. It is the most preferred wood energy source in Burundi, where wood is the principal source of domestic energy and accounts for 96.6% of total energy use, 77% of all charcoal being used in cities [95] . It is also extensively used as a construction material, wood industry (raw material and energy sources), and many other composite products. The second reason is the Government’s efforts. Besides the national reforestation programs, landowners (i.e., religious missions, public and private companies, households, and individuals) are constantly encouraged to plant more trees on their properties. Seeds and plants have long been distributed to the population freely.

The third reason is its high tolerance and adaptability to severe ecological conditions (desiccation, low soil fertility, degraded land, fire) that facilitate its adaptation and growth in different types of soil, climate, and topography of Burundi. The unpalatability of its leaves makes it non-palatable for insects and herbivorous animals [76] . Its high regeneration capacity, fast growth, short rotation, high yield per unit area and year coppicing ability, and easy silvicultural management make it an important cash crop [96] for many Burundians.

Despite its versatile importance, Eucalyptus presents some significant ecological challenges, despite the existing controversial views. Eucalyptus species are alleged to contribute to increasing the drying up of water sources and marshes, and that leads the Government to impose its uprooting and the cessation of its cultivation near rivers, water sources, and marshes. Its suspected contribution to soil desiccation, soil acidification, and allelopathic effects that are considered responsible for the reduction of biodiversity and productivity in planted environments arouses the distrust of agri-breeders. However, Burundians agree on the difficulty of finding a natural alternative to this Australian native plant species. By combining better silvicultural and energy yield, good quality wood, adaptability, and a socio-economic contribution, eucalyptus’s versatile importance seems to mask criticism of its disadvantages. Accordingly, and from a broad scientific conviction [93] [94] [95] , it is possible to predict its long-term existence and availability in Burundi and many other countries with similar ecological and socio-economic conditions. Our respondents acknowledge that these plant species is incorporated into the lifestyle of many people. They are committed to preserving it to allow it to be permanently available in sufficient quantities.

b. Cymbopogon citratus.

Cymbopogon citratus, also known as Citronella or lemongrass, is known as a mosquito-repellent plant species. Its use is becoming more popular in Burundi, particularly in urban and semi-urban areas where it is much more cultivated in home yards. Its leaves are currently sold in many markets in the capital of Bujumbura. C. citrates is a perennial, fast-growing, and aromatic grass. Originating in tropical countries of Asia (Indochina, Indonesia, and Malaysia), Africa, and the Americas, it is widely cultivated worldwide, particularly in tropical regions [97] . Its most reported chemical components include terpenes, alcohols, ketones, aldehyde and esters, flavonoids, and phenolic compounds [98] . Its essential oil contains various bioactive constituents, including aromatic compounds (myrcene and limonene), antimicrobial compounds (i.e., citral), insecticidal compounds (i.e., geraniol), citronellol, geranyl acetate, neral, and nerol [99] , Terpinolene, Terpinol Methylheptenone [98] .

Citronnela insecticidal activity is not limited to controlling mosquitoes. Studies reported the same activity against houseflies, Musca domestica, Phemacoccus solenopsis (a damaging insect for cotton crops, vegetables, and fruits), house dust mites, Dermatophagoides farinae and Dermatophagoides pteronyssinus, with more efficiency than Azadirachta indica (neem) leaf extract [100] .

Citronella extracts mainly the essential oil, can be used to counteract harmful microorganisms, including bacteria (i.e. Pseudomonas fluorescens, Salmonella enteritidis, Micrococcus luteus, Serratia marcescens [101] , Staphylococcus aureus, Salmonella typhi, Bacillus aureus, Escherichia coli [102] , Enterobacter cloacae and E. aerogenes [103] , Shigella flexneri, Yeast [99] and fungi [98] [99] [104] , i.e., Candida krusei, Candida albicans, Penicillium aurantiogriseum, Penicillium expansum, Penicillium chrysogenum, and Penicillium italicum [101] , Aspergillus species (i.e., Aspergillus ocraceus, A.orizae, A. fumigatus and A. parasiticus) [105] , Aspergillus niger, A. terreus, A. flavus and Penicilium citrinum [106] , Scropulariopsis brevicaulis, Fusarium poae, F. verticillioides, Trichophyton mentagrophytes, T. rubrum, Epidermophyton floccosum and Microsporum gypseum [107] , Microsporum canis [108] , Malassezia furfur [109] , Candida oleophila, Hansenula anomala, Saccharomyces cerevisiae, S. uvarum, Schizosaccharomyces pombe, and Metschnikowia fructicola [110] , Alternaria alternata, Fusarium oxysporum and Penicillium roquefortii [111] , etc. Their impressive anti-amoebic, anti-diarrheal, anti-filarial, and anti-inflammatory [97] [98] [104] activities have attracted more scientific and public attention. C. citratus is involved in improving gut/colon function and boosting the immune system.

Due to its anti-microbial activities, including the anti-food spoiling microorganisms activity, lemongrass essential oil can efficiently serve as a natural preservative of food [112] , i.e., bakery products, vegetables [110] , and yogurt [99] , etc., that represents a great opportunity in food technology. Thus, it constitutes a promising alternative to synthetic microbicides and other sanitation techniques in food storage and packaging. However, their effectiveness is microbial and dose-dependent [99] .

Lemongrass is also used to treat coughs, elephantiasis, influenza, headache, arthritis, leprosy, and malaria [113] . In-depth studies are conducted to confirm and divulge their various other effects such as anti-diabetic [104] , anti-cancer [114] , anti-obesity, anti-nociceptive, free radical scavengers, and anti-oxidant activities [97] [110] , anti-mutagenicity, hypoglycemic, neurobehavioral effect [98] [115] , anti-VIH (anti oral trash due to candida albicans), anti-hypertension, anti-tumor, anti-amebic, anti-ascaris, hypocholesterolemic, hypolipidemic, and larvicidal activities.

As reviewed by Shah and colleagues [98] , the chemical composition of an essential oil varies depending mostly on the nature and age of the plant and the geographical origin. Therefore, as the bioactivity of essential oil depends on its phytochemical constituents, studies should be conducted to confirm the reported differences in the ethnopharmacology of C. citratus between countries. For example, it was shown that C. citratus was applied to treat sore throat, empacho and as an emetic in Argentina, as antispasmodic, analgesic anti-inflammatory, antipyretic, diuretic, and as a sedative in Brazil, as a hypotensive for catarrh and rheumatism in Cuba, as renal antispasmodic and diuretic in Egypt. In India, C. citrates is used for the treatment of gastric troubles, cholera, severe headache and fever and as a sedative for the central nervous system. It also serves as an emmenagogue in Indonesia and Malaysia, as a condiment, as stomachic and anti-diabetes in Thailand, and for healing bone fractures and wounds in Minnesota (USA).

Due to its volatility and its typical lemony aroma, Cymbopogon citrates oil is used as a deodorant in cosmetics, toiletries, insecticides (bio-pesticides), and spend grass in the agricultural. It plays an essential role in the pharmaceutical and chemical industry where it is incorporated in the manufacture of various products, including cosmetics, perfumes, fragrances, detergents, soaps, and aftershaves, and the food industry where it serves as a culinary flavoring [97] .

More importantly, C. citratus application is of great opportunity in plant protection (as a repellent, bio-fungicide, bio-insecticide, and bio-herbicide, etc.) [103] and in the livestock sector (feed additives, feed conservation, veterinary medicine purposes). Feed supplementation with lemon grass leaves allows for improving daily animal growing rate [116] [117] and health [117] . In fact, regarding nutritional value, C. citratus contains various nutritional elements, including vitamins A and C, folic acid, iron, copper, magnesium, potassium, manganese, calcium, and zinc [118] which are essential for both human and animal nutrition. In vitro experiments have shown that lemongrass oil may efficiently help modulate rumen fermentation. The latter consists of reducing methanogenesis and ammonia nitrogen production irrespective of dietary substance [119] that contribute to reducing greenhouse gas production.

In Burundi, tea made from Citronella leaves, alone or in combination with extracts of Zingiber officinalis rhizome serves to treat flu, colds, and headaches. Very recently, citronella leaves were combined with eucalyptus and Ginger and taken orally (as liquor extracts) or by inhaling vapors as a potential remedy for Coronavirus 2019. Citronella is also used as an ornamental plant and for agricultural purposes (i.e., as spent grass in coffee plantations). From our survey, the essentiality of Cymbopogon citratus, including its uses as an antibiotic, food additive, food preservation, bioherbicide, etc., is very ignored in the study area.

From the survey and literature, C. citrates is not only associated with broad health-beneficial effects, but it is appropriate to point out some of its negative impacts. Prolonged use of extracts from this plant may lead to some side effects, including nephrotoxicity effects, particularly due to ethanolic extract [120] , and derma-toxicity effects associated with its skin irritant compounds [121] . The plant is also associated with allelopathic effects. Plant species such as Bidens pilosa and B. subalternans [122] [123] , Lactuca sativa [124] , Echinochloa crus-galli [123] [125] , Paniccum virgatum, Chlorus barbata, Euphorbia hirta, Stachytarpheta indica [126] are some of the reported affected. Inhibition of seed germination and seedling growth, cytotoxicity, and genotoxicity are among these allelopathic effects. The latter can serve as a basis for using Citronella extracts as bio-herbicides. Conversely, they can lead to an affliction on local biodiversity by reducing sensitive species abundance.

c. Tetradenia riparia

Tetradenia riparia is well-known in our study area as a mosquito-repellent plant. It is a deciduous shrub with intensely aromatic leafage and reaching 2 to 5 meters in height. It is widespread and well-adapted in Burundi, where it is grown in various places, mainly as fences for houses, home gardens, crop fields, fallows, roadsides, and paths. Tetradenia riparia possesses several chemical compounds. Its crude extracts and essential oil from the leaves stems, and flower buds contain mainly tannins, terpenoids, steroids, phenols, phenolic acids, flavonoids, saponins, anthocyanins, and alkaloids [127] . Numerous remarkable bioactive phytoconstituents have been well-documented [128] . These are potentially active against various pests, including blood-sucking insects, human and animal pathogens, and foodborne disease microorganisms.

Regarding the anti-insect properties, our respondents revealed that T. riparia is repellent for mosquitoes, flies, and fire ants while being an insecticide for jiggers. Tetradenia riparia is bioactive against Anopheles gambiae, Aedes aegypti, Leishmania species, Trypanosoma species, and Rhipicephalus sanguineus [129] [130] . The most reported sensitive bacteria [127] [131] include Bacillus cereus, Serratia liquefactions, Serratia marcescens, Salmonella typhi, Escherichia coli, Proteus vulgaris, Staphylococcus epidermidis, Bacillus subtilis, Enterococcus faecalis, Klebsiella pneumoniae, Lactobacillus casei, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mitis, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus sobrinus, Listeria monocytogenes, and Micrococcus kristinae, etc. Its antifungal activities are known for Candida albicans, Aspergillus versicolor, Penicillium ochrochloron, Listeria monocytogenes [131] , Mycobacterium smegmatis, Microsporum canis, Trichophyton mentagrophytes, etc. In addition, T. riparia is traditionally applied for its anti-viral activity, such as the potential anti-VIH/AIDs activity [128] . Due to its anti-microbial activity, T. riparia may be used against food-spoiling organisms, i.e., as a protective agent in cereals, legumes, or beans in warehouses.

Owing to the immense diversity and efficacy of its bioactive phytochemical constituents, T. riparia is found in different countries, mainly in Africa (i.e., in Burundi, Ouganda, Kenya, Rwanda, Tanzania, and South Africa) and Brazil. In these countries, it is recognized as an efficient natural alternative to the synthetic chemical used for the fight against blood-sucking insects and pathogen vectors and for the treatment of a wide range of diseases. The latter include those originating from bacterial, fungal, and viral infections, parasites (i.e., tick, Leishmania, and helminths), such as respiratory problems, diabetes [128] , cough, diarrhea, fever, malaria, dengue [132] , mycoses, boils, abscesses. In the field of traditional medicine, it is used in healing hemorrhoids, ulcers, skin diseases like itching, scabies, psychotic, dyspepsia, excitement, blocked fallopian tubes, emetic, bilharzia, body rushes, and cleaning wounds [128] , as well as analgesic, anti-tumor, antioxidant, anti-cariogenic, anti-spasmodic, anti-pyretic, anti-allergic and anti-inflammatory, vermifuge, tennifuge, anti-amoeba, and Diuretic.

All these uses are not to be generalized for all countries or regions. In Burundi, Tetradenia riparia is mostly used to treat candidiasis, toothache, fever, diarrhea, inflammatory diseases [133] , flu, bronchitis, sore throat, cough, migraine, and headache.

During the recent COVID-19 pandemic, some Burundians tried to use it alone or in combination with Eucalyptus globulus, and Tetradenia urticifolia, as a natural remedy against this pandemic virus. The emerging use of natural product extracts from various medicinal plants as mitigation measures against the COVID-19 pandemic was adopted in different developing countries. However, a scientific basis is still required to evaluate its safety and efficacy [128] .

In other countries, T. riparia serves for the treatment of ear infections, eye infections, stomach, weakness in pregnancy [134] and febrile convulsions [135] in Uganda, pneumonia in the Democratic Republic of Congo, respiratory infections, chest pain, influenza, chronic cough, shortness of breath and a runny or blocked nose, headache, chills, cough, tiredness, sleep disorders or sleepless nights in South Africa [136] , parasitic worms, stomach-ache, fungal infections, chest rheumatism in Democratic Republic of Congo (DRC) [137] , wounds, sores and scabies in Tanzania [138] and DRC [137] , etc. Variation of their local uses depends on various factors, including the originality of people, culture, and beliefs [128] , the collection time, soil, plant growing location, climate, seasonal variation, altitude [132] [139] , etc.

In addition, considering the range of its medicinal virtues, T. riparia is less exploited in Burundi and other countries. From this study, three main reasons may justify the low adoption of this medicinal plant as a remedy for various medical conditions. Firstly, the level of knowledge of T. riparia bioactivity remains low, generally based on ancestral knowledge and oral transmission in general. Secondly, there is little interest among younger generations due to the widespread adoption of synthetic products that are easy to use. The third reason is the insufficient scientific basis for determining effectiveness, dosage, contra-indication, and the side-effects of each treatment.

Unlike certain plants with various allelopathic effects, T. riparia is well-appreciated in agroecology and agroforestry. It can serve as fodder, melliferous, anti-erosion plant, fertilizer, and shady, ornamental (due to its flowers) and for Crop protection against pests.

Owing to its various bio-active properties, together with its easy multiplication and adaptation to different habitats, its strong capacity for regeneration after cutting, and its agroforestry and agro-ecological interest, this plant should be recommended for domestication and multiplication projects with a view to continuous exploitation and in sufficient quantity.

d. Cedrela Odorata

Cedrela odorata, also named Spanish cedar, is identified as a mosquito-repellent plant in this study. It is a medium to large tree reaching more than 30m in height. It is naturally found in humid ecosystems, specifically in tropical forests and mesophilic mountain forests, with a large distribution in American tropical forests [140] , and a concerning spreading trend in West African natural forests [141] .

From physicochemical analyses [142] [143] [144] , C. odorata essential oil and extracts contain different chemical compounds, mainly sesquiterpene hydrocarbons (the most dominant [143] [144] ) and oxygenated sesquiterpenes. However, their phytochemical composition, quality, and quantity vary depending on the plant (sample) origin, the climate, and the extraction methods [142] [144] . Due to the bioactivity of some of its phytochemical components, C. odorata essential oil and extracts are of medical and ecological interest.

From our survey, C. odorata is acknowledged as a mosquito-repellent plant species despite the low consensus between respondents (ICs = 0.01). Recent studies have proven the effectiveness of using C. odorata extracts as a biological insecticide against termites (Reticulitermes sp.) [140] that are capable of devouring almost any type of wood and destroying habitat, wooden structures such as frames, floors and beams, furniture, fence posts, clothes, books, paper, racks, etc.

Volatile oil from the stem bark of C. odorata is an efficient protectant against Sitophilus zeamais (Coleoptera) [145] [146] , a cosmopolitan pest that seriously damages stored cereal grains (up to 30% yield loss) [146] . C. odorata extracts exhibited an anti-feedant activity and toxicity on the Ostrinia nubilalis Hubner, and Callosobruchus maculatus [145] , Anthonomus eugenii (a pepper weevil causing an annual economic loss estimated at 70 to 80 million dollars in Mexico) [147] .

Regarding the antimicrobial activity, sensitivity tests revealed C. odorata’s potential antibacterial and anti-fungal activities. Sensitive bacteria include Bacillus cereus [143] [148] , Staphylococcus aureus, Escherichia coli, Enterococcoccus faecalis [148] , Pseudomonas aeruginosa, Bacilus subtilis [149] , etc., and various and anti-fungal activity including for dermatophytes, candida species, Scytalidium dimidiatum, Malassezia species, and Pneumocystis carinii [150] , Aspergillus niger, Penicillium notatum, Mucor mucedo and Candida albicans [149] .

C. odorata is also involved in various other medical conditions. Extracts from its leaves and stems are used to relieve vomiting and indigestion and for hemorrhage control. The leaf infusion is a tooth and ear pain reliever, while the stem is an antipyretic and abortifacient [140] . It may also be involved in healing malaria, intestinal infection, intoxication, poor digestion, and kidney diseases [151] . In vitro and in vivo tests on rats showed the potentiality of using C. odorata as an anti-hyperglycemic, anti-diabetic, and anti-oxidant [152] .

Concerning the socio-economic role, C. odorata has great economic value. This is due to its attractive wood quality which makes it a commercially important tree species in many producing countries, including Latin America and the Caribbean. Due to its lightweight (specific gravity of 0.47), high strength, easy workability, low and even shrinkage [153] , rot- and termite-resistance, and insect-repellent smell, timber from C. odorata is suitable for many uses. C. odorata wood is used for light construction, furniture, ship construction, musical instruments (ex., guitar), plywood, interior design, veneer, cigar boxes, wardrobes, etc. C. odorata can also serve as a source of firewood and charcoal.

Ecologically, this plant, native to tropical America, has been introduced in other tropical regions worldwide, including Africa, Asia, and the Pacific Islands, mainly as a timber tree and a promising forest restoration species [154] . Thus, it is integrated into national reforestation programs or cultivated on private properties. Due to its numerous seeds and their easy dispersal, it can grow naturally.

C. odorata is on the IUCN (International Union for Conservation of Nature) Red List of Threatened Species in different parts of the Americas. However, it paradoxically exhibits invasive behavior in various countries [154] , with a remarkable impediment to natural forests, plantation, and the biodiversity of the environments where it develops [141] [154] [155] .

In Burundi, all agroforestry and silvicultural tree species are quasi-exotic. C. odorata is grown alongside other most disseminated tree species, including Grevillea robusta, Maesopsis eminii, and Alnus acuminate. All of them are often planted in association with shrubs such as Calliandra calothyrsus, Leucena lecocephala, etc., to contribute to wood production, non-wood forest products, agroforestry systems with the aim of water and soil conservation, increasing soil fertility and adapting to climate change.

Regarding the flies repellent plant species, the consensus indices of the informants vary from 0.01 to 0.302 (Table 1), the higher consensus indices being for Eucalyptus globules subsp. maidenii (CIs = 0.302) and Acanthus polystachyus var. polystachyus (CIs = 0.086). For this category of insect, only Aloe sp. has been cited as an insecticide. These plant species may indistinctively act against different fly species, including Musca domestica, Glossina spp, Stomoxys calcitrans, Haematobia irritans, and Tabanus sp., which are among the main vectors of pathogens [156] . Musca domestica alone is a vector of more than 100 different pathogens [156] [157] , including various foodborne pathogens. The latter comprise parasites such as Entamoeba, Ascaris, Enterobius, Strongyloides, Ancylostoma, Necator, Trichuris, Metastrongylus, Haematopinus, Cryptosporidium, Giardia, Taenia, Hymenolepis [158] and Habronema [159] . Conversely, Glossina spp. can transmit to humans and animals different Trypanosma species, including T. vivax, T. congolense, T. simiae, T. brucei, T. gambiense, and T. rhodesiens.

For plant species used as flea repellent, two insect repellent species, Cupressus sp. And Eleusine coralana, were cited, with respective consensus indices of 0.032 and 0.005 (Table 1). Cupressus sempervirens leaf extracts are very effective in controlling houseflies, while the smoke produced by burning the whole Cupressus lusitanica plant is used in Ethiopia to repel mosquitoes and houseflies [37] .

All the 15 identified anti-jigger plants are insecticides (Table 1). The highest consensus indices are associated with Solanum aculeastrum (CIs = 0.288) and Tephrosia vogelii (CIs = 0.072), whereas Momordica foetida exhibited the lowest consensus index (0.005). The following examples illustrate the multipurpose use of anti-jigger plants.

a. Solanum aculeastrum

Phytochemical analysis revealed that Solanum aculeastrum contains steroidal alkaloids, including β-solamarine, solamargine, solasonine, solasodine, solaculine, tomatine, and tomatidine [160] . Their associated bioactivity renders S. aculeastrum extracts efficient in fighting against not only insects but also parasites and pathogens, including bacteria species (i.e., Bacillus cereus, Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus pyogenes, Micrococcus kristinae) and fungal species (i.e., Aspergillus flavus, Penicillium notatum) [161] , protozoan species such as Leishmania, transmitted by sandflies of the genus Phlebotomus or Lutzomyia [11] . S. aculeastrum extracts are also reported as molluscides [160] [162] and antioxidants. S. aculeastrum berries have long been used as an alternative treatment to cancer [163] . However, its anticancer activity is still scientifically controversial [163] [164] . Fighting against jigger fleas helps prevent typhus and trench caused by infection by Rickettsia species transmitted to humans by insects.

Handling and using S. aculeastrum fruit extracts requires caution because they can cause health and ecotoxicological effects. Toxicity tests on male Wistar rats have revealed potential immunodeficiency and hypersensitivity to the aqueous extract of the fresh, unripe berries of S. aculeastrum [165] . It was reported acute toxicity symptoms, including hypoactivity, respiratory distress, and epistaxis. S. aculeastrum fruit extracts adversely affected the normal functioning of the blood, liver, and kidney. S. aculeastrum is one of the most common fish-poisoning plants. Its use, as a simple method of fishing, should be strictly avoided [166] . Its non-selective toxicity on aquatic organisms (i.e., insects, larvae, fishes, etc.) adversely affects the food chain and ecosystem functioning.

b. Tephrosia vogelii

Phytochemical analysis revealed the presence of various components such as retinoids, steroids, glucosides, tannins, rotenones isoflavones, chacones, flavanones, flavanols, prenylated flavonoids, alkaloids, saponins, balsam, cardiac glycosides, phenol and volatile oil on which relies its bioactive properties, i.e. Retinoid based entomotoxic properties, anti-bacterial, anthelminthic, and fish poison properties [167] . According to the World Agroforestry Centre [168] , rotenone was classified by the World Health Organization as a moderately hazardous or Class II pesticide. Thus, T. vogelii was widely applied to pest control before the invention of DDT (Dichlorodiphenyltrichloroethane) and is still usable in controlling pests in the field and storage or on domestic animals. Using Tephrosia vogelii crude leaf extracts to control jiggers and worms in livestock production systems has been rated equal to synthetic insecticide [169] . Compared to many synthetic pesticides, the most known advantage is its rapid breakdown (within 3 - 5 days after application [168] . Laboratory tests on mice exhibited acute toxicity of its crude methanolic leaf extract with similar effects to rotenone poisoning in mammals. Neurological disorders and hepatic, renal, and cardiac necrotic changes are among the most predominant effects [170] . Acute toxicity of extracts from T. vogelii was also observed in relevant species in aquaculture ponds, such as Rotifers, Cyclops, mosquito larvae [167] , water fleas (i.e., Daphnia magna, Crustacea), adult fishes, and fish larvae [167] [171] . From these few examples, Tephrosia vogelii and Solanum species should be carefully handled, for example, by preventing skin contact.

c. Tithonia diversifolia

In the study zone, Tithonia diversifolia is known and used as a jigger killer. Our informants are not well-informed on its other potentially beneficial properties, including the bioactivity against mosquito species (i.e., Anopheles gambiae, Aedes aegypti, and Culex quinquefasciatus), the antimalarial activity [172] , the bioactivity against root-knot nematodes (i.e., Meloidogyne incognita, M. arenaria, M. hapla, M. javanica, M. enterolobii) [173] and against ants (i.e., workers of Atta cephalotes L.) [174] . T. divesifolia is also used for its anti-inflammatory and analgesic activities compost [173] . Extracts from aerial parts of T. diversifolia contain terpenoids and flavonoids components [175] [176] [177] on which depends its traditional use for the treatment of malaria, diabetes, menstrual pain, diarrhea, hepatomas, hepatitis, hematomas, and wound healing [175] [178] . Owing to its free radical scavenger capacity and also by induction of protective cellular systems involved in cellular stress defenses and in adipogenesis of mesenchymal cells, T. diversifolia, exhibited health-promoting properties [175] . However, the use of T. diversifolia extracts for disease treatment may raise concerns over its safety [179] . Laboratory tests have shown that prolonged exposure to higher doses of T. diversifolia extracts adversely affects the kidneys and liver [179] [180] .

Ecologically, Tithonia diversifolia, considered an ornamental plant, has exhibited invasive behavior, particularly due to its rapid growth rates, the easy and massive seed dispersion by wind, animals, and water, high adaptation ability in various habitats (disturbed sites, fallows, and ruderal areas, along roadsides, watercourses, wastewater evacuation gutters, and waste disposal sites) and different field soil and moisture conditions. T. diversifolia is used as green manure for Nitrogen sources and is recommended as a key component of compos [173] .

Water extract from leaves, stems and roots of Tithonia diversifolia exhibited allelopathic effects on shoot growth, root growth, and seedling growth of various plants such as rice [181] [182] , maize [183] , cabbage, cucumber, lettuce, mung bean, tomato, oat, sorghum, wheat, crabgrass, slender amaranth, jointvetch, rice flatsedge, onion, radish, and itchgrass [182] .

Plants used as fire ant repellents have a consensus index between 0.01 and 0.712. The most cited plants within this category include Tetradenia riparia (ICs = 0.712), Musa sp. (CIs = 0.081), Tagetes minuta (CIs = 0.05), Solanecio mannii (CIs = 0.041). Other plant species have consensus indices of less than 0.05. Fumigation of the whole Tagetes minuta plant, in a mixture with Eucalyptus globulus leaves, keeps insects away from attacking living spaces [37] . Concerning Solanecio mannii, its leaves contain volatile compounds that have repellent properties that help control subterranean termites, Macrotermes natalensis [184]