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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article">
  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">OJE</journal-id>
      <journal-title-group>
        <journal-title>Open Journal of Ecology</journal-title>
      </journal-title-group>
      <issn pub-type="epub">2162-1985</issn>
      <publisher>
        <publisher-name>Scientific Research Publishing</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.4236/oje.2024.144016</article-id>
      <article-id pub-id-type="publisher-id">OJE-132379</article-id>
      <article-categories>
        <subj-group subj-group-type="heading">
          <subject>Articles</subject>
        </subj-group>
        <subj-group subj-group-type="Discipline-v2">
          <subject>Earth&amp;Environmental Sciences</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>


          Growth, Population Parameters and Stock Status of &lt;i&gt;Sarotherodon galilaeus&lt;/i&gt; in Samandeni Reservoir, Burkina Faso

        </article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author" xlink:type="simple">
          <name name-style="western">
            <surname>Nomwine</surname>
            <given-names>Da</given-names>
          </name>
          <xref ref-type="aff" rid="aff1">
            <sup>1</sup>
          </xref>
          <xref ref-type="corresp" rid="cor1">
            <sup>*</sup>
          </xref>
        </contrib>
        <contrib contrib-type="author" xlink:type="simple">
          <name name-style="western">
            <surname>Raymond</surname>
            <given-names>Ouedraogo</given-names>
          </name>
          <xref ref-type="aff" rid="aff1">
            <sup>1</sup>
          </xref>
        </contrib>
        <contrib contrib-type="author" xlink:type="simple">
          <name name-style="western">
            <surname>Mahamoudou</surname>
            <given-names>Minoungou</given-names>
          </name>
          <xref ref-type="aff" rid="aff2">
            <sup>2</sup>
          </xref>
        </contrib>
        <contrib contrib-type="author" xlink:type="simple">
          <name name-style="western">
            <surname>Adama</surname>
            <given-names>Oueda</given-names>
          </name>
          <xref ref-type="aff" rid="aff2">
            <sup>2</sup>
          </xref>
        </contrib>
      </contrib-group>
      <aff id="aff2">
        <addr-line>Animal Biology and Ecology Laboratory, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso</addr-line>
      </aff>
      <aff id="aff1">
        <addr-line>Institute for the Environment and Agricultural Research, National Center for Scientific and Technological Research, Ouagadougou, Burkina Faso</addr-line>
      </aff>
      <pub-date pub-type="epub">
        <day>11</day>
        <month>04</month>
        <year>2024</year>
      </pub-date>
      <volume>14</volume>
      <issue>04</issue>
      <fpage>257</fpage>
      <lpage>273</lpage>
      <history>
        <date date-type="received">
          <day>23,</day>
          <month>February</month>
          <year>2024</year>
        </date>
        <date date-type="rev-recd">
          <day>8,</day>
          <month>April</month>
          <year>2024</year>
        </date>
        <date date-type="accepted">
          <day>11,</day>
          <month>April</month>
          <year>2024</year>
        </date>
      </history>
      <permissions>
        <copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement>
        <copyright-year>2014</copyright-year>
        <license>
          <license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p>
        </license>
      </permissions>
      <abstract>
        <p>


          Mango tilapia, &amp;lt;i&amp;gt;Sarotherodon galilaeus&amp;lt;/i&amp;gt; is one of the most caught fish species in the Samandeni multi-species fishing sites of which, few data on its biology and exploitation are available. The study aimed to Assess the stock status of &amp;lt;i&amp;gt;S&amp;lt;/i&amp;gt;.&amp;lt;i&amp;gt; galilaeus&amp;lt;/i&amp;gt;. Sampling was conducted from March, 2021 to February 2022 based on commercial fish catches to analyze growth parameters, first sexual maturity size and harvest status of the stock. A total of 572 specimens including 297 females and 275 males were examined. The stock assessment was performed by using the Length based Bayesian method of Biomass (LBB) and that of growth by the ELEFAN method. The growth parameters showed a seasonality of growth and females appeared to grow faster than males. On the other hand, males had a greater asymptotic length than females. Results on the estimated length of fish at first maturity showed that females firstly reached the maturity compared to males. The relative biomass (B/B&amp;lt;sub&amp;gt;0&amp;lt;/sub&amp;gt;) estimated for the stock was higher than the relative biomass that produces maximum sustainable yield (B&amp;lt;sub&amp;gt;MSY&amp;lt;/sub&amp;gt;/B&amp;lt;sub&amp;gt;0&amp;lt;/sub&amp;gt;) indicating healthy biomass. In addition, the length at first sexual maturity was less than the length at the first catch, indicating the absence of overfishing of growth. In addition, extending the study to the various stocks of the reservoir would be important for the sustainable management of the Samandeni high economic fishing area.

        </p>
      </abstract>
      <kwd-group>
        <kwd>Growth</kwd>
        <kwd> Stock Status</kwd>
        <kwd> &lt;i&gt;Sarotherodon galilaeus&lt;/i&gt;</kwd>
        <kwd> Samandeni Reservoir</kwd>
        <kwd> Maturity</kwd>
      </kwd-group>
    </article-meta>
  </front>
  <body>
    <sec id="s1">
      <title>1. Introduction</title>
      <p>
        The Samandeni reservoir is a hydroelectric reservoir located on the Volta River in the Upper Basins region of Burkina Faso. It offers opportunities in various sectors, including agriculture, fishing and fish farming. Since its opening for fishing in 2019, Samandeni reservoir has been one of the main supports of the fisheries production in Burkina Faso. However, the ever-increasing demand for fish and fish products increases the fishing pressure on natural fish population, which sometimes leads to a significant depletion of the natural fish stock. In this context, in order to alleviate fishing pressure and to ensure its sustainability, it is essential to assess the key biological parameters and the status of fish stocks in this ecosystem. In addition, management plans must be established based on the stock assessment information [<xref ref-type="bibr" rid="scirp.132379-ref1">1</xref>] and scientific approaches [<xref ref-type="bibr" rid="scirp.132379-ref2">2</xref>] . Many commercially exploited stocks and particularly fish stocks in inland fisheries also lack quantitative assessments and reliable estimates of the fish stock status [<xref ref-type="bibr" rid="scirp.132379-ref1">1</xref>] [<xref ref-type="bibr" rid="scirp.132379-ref3">3</xref>] [<xref ref-type="bibr" rid="scirp.132379-ref4">4</xref>] . Furthermore, fishery landings in Samandeni Reservoir are primarily in the form of a multi-species complex, which poses a challenge to record species-specific catch and effort details, yet are required for regular stock assessment using conventional stock assessment models. Therefore, assessment of life history characteristics, such as growth, population demographics, and reproductive characteristics of the most abundant fish species, is of critical importance for sustainable fisheries management. In the Samandeni reservoir, Sarotherodon galilaeus is the most commercially important species. Indeed, it is an abundant fish in the reservoir where it is part of the main commercial catch [<xref ref-type="bibr" rid="scirp.132379-ref5">5</xref>] . In addition, a study conducted before the reservoir was opened to fishing showed S. galilaeus to be one of the most abundant fish species in this ecosystem [<xref ref-type="bibr" rid="scirp.132379-ref6">6</xref>] . Despite its considerable economic value, only one study was conducted on the biology of this species in Samandeni Reservoir prior to its exploitation [<xref ref-type="bibr" rid="scirp.132379-ref7">7</xref>] . Other studies were also conducted on the biology of this species in some water bodies in Burkina [<xref ref-type="bibr" rid="scirp.132379-ref8">8</xref>] . Although, data available from other water bodies can be used for fisheries management of S. galilaeus, the importance of demographic parameters specific to Samandeni Reservoir cannot be minimized. Therefore, estimates of life history characteristics and population demographics of the most abundant fish species in Samandeni Reservoir are warranted. Moreover, ecological changes observed in this reservoir over the years, as well as intensive fishing pressures, have influenced the population parameters of various fish species. Thus, this study was undertaken to determine the growth and population parameters of S. galilaeus in Samandeni Reservoir using length-frequency data. We therefore analyzed the population dynamics of S. galilaeus based on 12 months period of catch data collected from fishermen by estimating growth, mortalities, some reproductive characteristics and exploitation status of the stock and formulated recommendations to ensure the sustainable management of the fish species in the reservoir.
      </p>
    </sec>
    <sec id="s2">
      <title>2. Material and Methods</title>
      <sec id="s2_1">
        <title>2.1. Study Site and Fish Sampling</title>
        <p>
          The newly created reservoir of Samandeni is located in the Volta catchment of Burkina Faso between latitudes 11˚23' and 11˚19' North and longitudes 4˚34' and 4˚46' West (<xref ref-type="fig" rid="fig1">Figure 1</xref>). This waterbody got the status of Ramsar site in October 2020. It has 153 km<sup>2</sup> wide with a storing water capacity of more than one billion of m<sup>3</sup>. The fish specimens were collected from the catch of local fishers on a monthly basis from March 2021 to February 2022 by considering nine sampling sites as shown in <xref ref-type="fig" rid="fig1">Figure 1</xref>. Fish species were identified by using the standard identification key given by [<xref ref-type="bibr" rid="scirp.132379-ref9">9</xref>] . In the Samandeni reservoir, the fishermen are known to use a variety of fishing methods such as gillnets with a mesh size of 10 to 40 mm, longlines, traps and cast net.
        </p>
      </sec>
      <sec id="s2_2">
        <title>2.2. Sample Processing and Measurements</title>
        <p>
          Fish were randomly sampled from several fishermen to ensure a good representation of classes size of the samples. Each specimen was examined on the same day they were collected to avoid error in the morphometric measurements posed by fixation. The total length (TL in cm) of each fish was measured by using empirical approach to calculate length frequency (LF) distributions according to [<xref ref-type="bibr" rid="scirp.132379-ref10">10</xref>] and to estimate growth parameters. The body weight (W) of each specimen was also measured (0.1 g) using an electronic balance.
        </p>
      </sec>
      <sec id="s2_3">
        <title>2.3. Growth Parameters Estimation</title>
        <p>
          A monthly collected value of TL, grouped into class intervals of 2 cm according to [<xref ref-type="bibr" rid="scirp.132379-ref10">10</xref>] empirical approach, was used to estimate the fish population demographic parameters. The asymptotic length (L<sub>inf</sub>), the coefficient of growth (K), the amplitude of the growth oscillations (C), and the fraction of a year from which the sine wave oscillation begins (t<sub>s</sub>) were estimated using TropFishR, an R package ver. 3.5.1 for tropical fisheries analysis [<xref ref-type="bibr" rid="scirp.132379-ref11">11</xref>] . Since we collected length-frequency (LF) data, the electronic length frequency analysis method (ELEFAN) was employed to estimate the population demographic parameters [<xref ref-type="bibr" rid="scirp.132379-ref12">12</xref>] . A bootstrapped ELEFAN with genetic algorithm optimization function developed by [<xref ref-type="bibr" rid="scirp.132379-ref13">13</xref>] was applied to analyze LF data, allowing the assessment of the uncertainties around the growth parameters estimate. Growth was modeled based on Von Bertalanffy growth function (VBGF) and a seasonally oscillating VBGF was used to assess the growth parameters, which are attributed to changes in water temperature, precipitation and to the availability of food [<xref ref-type="bibr" rid="scirp.132379-ref14">14</xref>] . The seasonally oscillating VBGF [<xref ref-type="bibr" rid="scirp.132379-ref15">15</xref>] used is given as:
        </p>
        <p>L t = L i n f ( 1 − e − ( k ( t − t 0 ) + S ( t ) − S ( t 0 ) ) ) (1)</p>
        <p>
          With S ( t ) = ( C K / 2 π ) sin 2 π ( t − t s ) , S ( t 0 ) = ( C K / 2 π ) sin 2 π ( t 0 − t s ) , and L<sub>t</sub> is the length -at -age t, L<sub>inf</sub> is the asymptotic length, K is the von Bertalanffy growth constant, and t<sub>0</sub> is the theoretical age when length equals zero, C is a constant indicating the amplitude of the oscillation, typically ranging from 0 to 1, and t<sub>s</sub> is the fraction of a year where the sine wave oscillation begins (turns positive). A value of C = 1, growth stops completely once a year at a point in the annual cycle while values of C ranging between 0 and 1 results in slowed but not stopped, growth [<xref ref-type="bibr" rid="scirp.132379-ref16">16</xref>] .
        </p>
      </sec>
      <sec id="s2_4">
        <title>2.4. Estimation of Mortalities Coefficient</title>
        <p>
          Total mortality coefficient (Z) is estimated using the length-converted catch curve method [<xref ref-type="bibr" rid="scirp.132379-ref17">17</xref>] from the pooled length frequency data for the study period. The slope (b) of the curve with sign change gives the total mortality. The natural mortality rate (M) was calculated using [<xref ref-type="bibr" rid="scirp.132379-ref18">18</xref>] equation as follows:
        </p>
        <p>M = 4.118 K 0.73 L i n f 0.33 (2)</p>
        <p>Fishing mortality coefficient (F) was estimated by subtracting the value of natural mortality coefficient (M) from the value of total mortality coefficient (Z) as follow:</p>
        <p>F = Z − M (3)</p>
      </sec>
      <sec id="s2_5">
        <title>
          2.5. Length at First Capture (L<sub>c</sub>)
        </title>
        <p>
          The body length at first capture (L<sub>c</sub>) is the average selection length and was estimated by interpolation of the cumulative catch curve. The probability of capture for each length class was obtained from the backward extrapolation of the linearized capture curve, according to [<xref ref-type="bibr" rid="scirp.132379-ref17">17</xref>] . The extrapolated points from the length-converted catch curve were used to estimate the catch probability using the moving average method to estimate the length at first capture (L<sub>c</sub>) by linear interpolation.
        </p>
        <p>
          The capture probability was estimated by backward extrapolation, of the descending limb of the length-converted catch curve. A selectivity curve was generated using linear regression fitted to the ascending data points from a plot of the probability of capture against length, which was used to derive values of the lengths at capture at probabilities at 50%, 75% and 95% [<xref ref-type="bibr" rid="scirp.132379-ref12">12</xref>] .
        </p>
      </sec>
      <sec id="s2_6">
        <title>2.6. Length at First Sexual Maturity</title>
        <p>
          The body length at which 50% of the individuals were mature (L<sub>m</sub>) was estimated by fitting the logistic function of a non-linear regression relating the proportions of mature individuals (P) and total length (LT) of fish. The gonads maturity stages were determined by macroscopic observation of gonads according to [<xref ref-type="bibr" rid="scirp.132379-ref19">19</xref>] . Specimens falling within Stage II and above gonad maturity were used for L<sub>m</sub> determination. The percentage of mature individuals in each length class was calculated based on the total number of individuals in each length class. The sigmoid curve was fitted using a standard logistic regression model as follows:
        </p>
        <p>P = 1 1 + exp − ( α L T + β ) (4)</p>
        <p>
          where α and β are the model parameters. Following a logarithmic transformation of the previous formula and, by substituting P = 50% in the equation, L<sub>m</sub> is obtained by:
        </p>
        <p>L m = − α β (5)</p>
      </sec>
      <sec id="s2_7">
        <title>2.7. Biological Reference Points and Stock Status</title>
        <p>
          The stock status was estimated using the LBB method that is a recent approach developed by [<xref ref-type="bibr" rid="scirp.132379-ref20">20</xref>] to access the stock, which uses LF data as input and applies a Bayesian Monte Carlo Markov Chain (MCMC) method to estimate indicators of stock status. The LBB model is suitable for species that grow throughout their life, such as most economic fish and invertebrates [<xref ref-type="bibr" rid="scirp.132379-ref20">20</xref>] . It estimates the asymptotic body length (L<sub>inf</sub>), the first catch body length (L<sub>c</sub>), the relative natural mortality (M/K) and the relative fishing mortality (F/M) in the age range represented by LF samples. With these parameters as input, standard fisheries equation can be used to estimate the depletion or the current exploited biomass with regards to unexploited biomass (B/B<sub>0</sub>). These parameters also allow the estimation of the length at first capture that would maximize catch and biomass for the given fishing effort (L<sub>c</sub><sub>_opt</sub>), and the estimation of a proxy for the relative biomass capable of producing maximum sustainable yields (B<sub>MSY</sub>/B<sub>0</sub>). The method uses a pre-specified set of priors, but users can manually incorporate priors for asymptotic length (L<sub>inf</sub>), length at first capture (L<sub>c</sub>), and relative natural mortality (M/K) from local studies. The L<sub>inf</sub> priors were estimated from the median maximum length rather than the absolute maximum length. The M/K prior is set as 1.5, and L<sub>c</sub> is estimated from the catch curve generated from the input LF data. We used a first maturity body length (L<sub>m</sub>) estimates by this study and computed the numeric percentage of specimen in the catches larger than L<sub>m</sub>. In the present study the basic equations are given below, however, for more details on the LBB method see [<xref ref-type="bibr" rid="scirp.132379-ref20">20</xref>] . <xref ref-type="table" rid="table1">Table 1</xref> stands for the basic information and priors (L<sub>inf</sub>, L<sub>c</sub>, Z/K, M/K, F/K, and α) for the LBB analysis.
        </p>
        <p>The analysis presented was done using R code (LBB_33a. R), downloaded from http://oceanrep.geomar.de/44832/ following the guidelines provided in the new user guide of the code.</p>
        <p>First of all, a major assumption of the LBB method is that growth in length follows VBGF:</p>
        <p>L t = L i n f [ 1 − e − K ( t − t 0 ) ] (6)</p>
        <p>
          where L<sub>t</sub> is the length at age t, L<sub>inf</sub> is the asymptotic length, K is the growth rate, t<sub>0</sub> is theorical age at length null.
        </p>
        <p>The function of the selectivity ogive (here assumed trawl like) is given by the following equation:</p>
        <p>S L = 1 1 + e − α ( L − L c ) (7)</p>
        <p>
          where S<sub>L</sub> is the fraction of individuals that are retained by the gear at length L, and α describes the steepness of the ogive and L<sub>c</sub> the body length at first capture.
        </p>
        <p>
          To estimate L<sub>inf</sub>, L<sub>c</sub>, α, M/K and F/K simultaneously the following equation fitting the whole fishing quantity curve are use:
        </p>
        <p>N L i = N L i − 1 ( L i n f − L i L i n f − L i − 1 ) M K + F K S L i (8)</p>
        <p>C L i = N L i S L i (9)</p>
        <p>
          where L<sub>i</sub> is the number of individuals at length i, L<sub>i</sub><sub>−1</sub> is the number at the previous length, where C refers to the number of individuals vulnerable to the gear and all other parameters are as described above.
        </p>
      </sec>
    </sec>
    <sec id="s3">
      <title>3. Results</title>
      <sec id="s3_1">
        <title>3.1. Length Frequency Distribution</title>
        <p>
          A total of 2844 fish specimen were sampled from March 2021 to February 2022. As shown in <xref ref-type="fig" rid="fig2">Figure 2</xref>, the length distribution was unimodal, and ranged from
        </p>
        <table-wrap id="table1" >
          <label>
            <xref ref-type="table" rid="table1">Table 1</xref>
          </label>
          <caption>
            <title> Basic information of S. galilaeus and prior for the LBB method</title>
          </caption>
          <table>
            <tbody>
              <thead>
                <tr>
                  <th align="center" valign="middle"  colspan="3"  >Length</th>
                  <th align="center" valign="middle"  colspan="6"  >Prior</th>
                </tr>
              </thead>
              <tr>
                <td align="center" valign="middle" >Min</td>
                <td align="center" valign="middle" >Mean</td>
                <td align="center" valign="middle" >Max</td>
                <td align="center" valign="middle" >
                  L<sub>inf</sub>
                </td>
                <td align="center" valign="middle" >Z/K</td>
                <td align="center" valign="middle" >M/K</td>
                <td align="center" valign="middle" >F/K</td>
                <td align="center" valign="middle" >
                  L<sub>c</sub> (cm)
                </td>
                <td align="center" valign="middle" >alpha</td>
              </tr>
              <tr>
                <td align="center" valign="middle" >5.5</td>
                <td align="center" valign="middle" >18.2</td>
                <td align="center" valign="middle" >30.9</td>
                <td align="center" valign="middle" >32.3</td>
                <td align="center" valign="middle" >2.6</td>
                <td align="center" valign="middle" >1.5</td>
                <td align="center" valign="middle" >1.11</td>
                <td align="center" valign="middle" >12.8</td>
                <td align="center" valign="middle" >14.6</td>
              </tr>
            </tbody>
          </table>
        </table-wrap>
        <p>7.8 to 27.4 cm. Analysis shows that the mean and median are practically confused for male and female, indicating that the length frequencies are normally distributed in these samples. The mean length is 16.3 and 16.9 cm respectively for male and female, showing that male is longer than female on average.</p>
      </sec>
      <sec id="s3_2">
        <title>3.2. Asymptotic Length and Growth Rate</title>
        <p>
          The parameters of growth as estimated from the maximum density shown in <xref ref-type="table" rid="table2">Table 2</xref> and illustrated by <xref ref-type="fig" rid="fig3">Figure 3</xref> and <xref ref-type="fig" rid="fig4">Figure 4</xref>. The growth rate (K) was 0.94 yrs<sup>−1</sup> for male fish while that of females were 0.68 yrs<sup>−1</sup>. The asymptotic length (L<sub>inf</sub>) was 29.4 and 27.4 cm respectively for males and females. <xref ref-type="fig" rid="fig4">Figure 4</xref> shows the reconstructed length distribution frequency diagram of the assessed fish species. The value of growth oscillation intensity (C) suggests seasonality in the growth of the Mango tilapia and the summer point (t<sub>s</sub>) was estimated as 0.58 (July) and 0.41 (June) as shown in <xref ref-type="fig" rid="fig3">Figure 3</xref>. The estimated K values were similar between male and female fish: 0.72 and 0.61/yrs for female and male respectively (<xref ref-type="fig" rid="fig4">Figure 4</xref>).
        </p>
        <p>The maximum density estimates of the parameter t anchor was 0.57, and 0.64, representing the months of September, where yearly repeating growth curves cross length equal to zero for female and male of S. galilaeus respectively.</p>
      </sec>
      <sec id="s3_3">
        <title>3.3. Mortality Estimates</title>
        <p>
          The total rates of mortality (Z) obtained through the length-converted catch curves were 2.64 yrs<sup>−1</sup>. The parameters of natural mortality rate (M) estimated by Then (2015) empirical equation were 1.33 yrs<sup>−1</sup>. For Samandeni reservoir, the fishing mortality rates (F) were 1.27 yrs<sup>−1</sup>.
        </p>
        <table-wrap id="table2" >
          <label>
            <xref ref-type="table" rid="table2">Table 2</xref>
          </label>
          <caption>
            <title>
              Life history parameters of Sarotherodon galilaeus collected from commercial fishermen during March 2021-Febraury 2022 from Samandeni reservoir. L<sub>inf</sub> (cm): Asymptotic length; K (yr∙s<sup>−1</sup>): Growth rate; t_anchor: Fraction of the year where the length is zero; C: Intensity of the oscillation; t<sub>s</sub>: Fraction of the year where growth is positive
            </title>
          </caption>
          <table>
            <tbody>
              <thead>
                <tr>
                  <th align="center" valign="middle" >Parameters</th>
                  <th align="center" valign="middle" >Male</th>
                  <th align="center" valign="middle" >Female</th>
                </tr>
              </thead>
              <tr>
                <td align="center" valign="middle" >
                  L<sub>inf</sub> (cm)
                </td>
                <td align="center" valign="middle" >29.4</td>
                <td align="center" valign="middle" >27.4</td>
              </tr>
              <tr>
                <td align="center" valign="middle" >
                  K (yrs<sup>−1</sup>)
                </td>
                <td align="center" valign="middle" >0.61</td>
                <td align="center" valign="middle" >0.72</td>
              </tr>
              <tr>
                <td align="center" valign="middle" >t_anchor</td>
                <td align="center" valign="middle" >0.64</td>
                <td align="center" valign="middle" >0.57</td>
              </tr>
              <tr>
                <td align="center" valign="middle" >C</td>
                <td align="center" valign="middle" >0.47</td>
                <td align="center" valign="middle" >0.62</td>
              </tr>
              <tr>
                <td align="center" valign="middle" >
                  t<sub>s</sub>
                </td>
                <td align="center" valign="middle" >0.58</td>
                <td align="center" valign="middle" >0.41</td>
              </tr>
            </tbody>
          </table>
        </table-wrap>
      </sec>
      <sec id="s3_4">
        <title>3.4. The Body Length at First Capture</title>
        <p>
          The length at first capture (L<sub>c</sub>) was 17 cm. <xref ref-type="fig" rid="fig5">Figure 5</xref> shows the probability of capture in terms of age.
        </p>
      </sec>
      <sec id="s3_5">
        <title>3.5. Body Length at First Sexual Maturity</title>
        <p>
          The body length at first sexual maturity of the Mango tilapia, S. galilaeus was 12.5, 12.1 and 13.4 cm for both sexes, male and female, respectively (<xref ref-type="fig" rid="fig6">Figure 6</xref>). The least matured male and female in the population were 8.3 and 7.8 cm TL, respectively. The difference is not statistically significant although females appear to reach sexual maturity early.
        </p>
      </sec>
      <sec id="s3_6">
        <title>3.6. Stock Status</title>
        <p>
          Results for specific evaluation of LBB method are shown in <xref ref-type="fig" rid="fig7">Figure 7</xref>. The blue line in the figure represents the fit of the data and the LBB estimation, and the red line is the evaluation from the LBB method of population resources. The parameters L<sub>mean</sub>/L<sub>opt</sub>, L<sub>c</sub>/L<sub>c</sub><sub>_opt</sub>, L<sub>95th</sub>/L<sub>inf</sub>, B/B<sub>0</sub>, B/B<sub>MSY</sub>, B<sub>MSY</sub>/B<sub>0</sub> and F/M were obtained (<xref ref-type="table" rid="table3">Table 3</xref>). The estimated B/B<sub>MSY</sub> was 1.2, which confirmed the healthy condition of S. galilaeus stock and indicated the safe biomass level capable of producing the MSY. The calculated B/B<sub>0</sub> (0.41) was near to the reference limit for S galilaeus stock biomass and denoted that the biomass was in the safe condition, whereby 59% of the wild stock of this species were harvested. The assessed B/B<sub>0</sub> was larger than B<sub>MSY</sub>/B<sub>0</sub> (0.34), and F/M was 0.81, which indicated an underfishing condition. The output result for L<sub>c</sub>/L<sub>c</sub><sub>_opt</sub> and L<sub>mean</sub>/L<sub>opt</sub> was 1, showing the healthy stock with the fishing of prominent individuals. The output result of L<sub>95th</sub>/L<sub>inf</sub> (=0.91) is close to one, indicating the presence of the significant number of individuals in the stock. In addition, a smaller estimation was observed for L<sub>c</sub> (17) than L<sub>c</sub><sub>_opt</sub> (20), indicating that overfishing increased and recommended that the first fish catch was more extensive. Furthermore, the B/B<sub>0</sub> &gt; B<sub>MSY</sub>/B<sub>0</sub> and L<sub>c</sub> &lt; L<sub>c</sub><sub>_opt</sub> suggested that the existing fishing pressure and mesh sizes may influence the biomass. However, it is recommended that slightly increasing the mesh size for fishing may be beneficial for the sustainability of the S. galilaeus population in the Samandeni.
        </p>
      </sec>
    </sec>
    <sec id="s4">
      <title>4. Discussion</title>
      <p>
        The growth curve in males is characterized by the following von Bertalanffy parameters: L<sub>∞</sub> = 29.4 cm and K = 0.6 yrs<sup>−1</sup>. For females, the same values were 27.4 and 0.7 respectively. In terms of growth, the results of the present study indicated that there were differences in the growth curves of S. galilaeus by sex and that these differences are mainly reflected in the mean asymptotic total length (L<sub>∞</sub>) since the growth constants (K) were almost the same. A comparative analysis of the fish population parameter estimates for different settings is provided in
      </p>
      <table-wrap id="table3" >
        <label>
          <xref ref-type="table" rid="table3">Table 3</xref>
        </label>
        <caption>
          <title> Estimated LBB for S. galilaeus using length frequency (LF)</title>
        </caption>
        <table>
          <tbody>
            <thead>
              <tr>
                <th align="center" valign="middle" >
                  L<sub>c</sub> (cm)
                </th>
                <th align="center" valign="middle" >
                  L<sub>c_opt</sub> (cm)
                </th>
                <th align="center" valign="middle" >
                  L<sub>mean</sub>/L<sub>opt</sub>
                </th>
                <th align="center" valign="middle" >
                  L<sub>c</sub>/L<sub>c_opt</sub>
                </th>
                <th align="center" valign="middle" >
                  L<sub>95th</sub>/L<sub>inf</sub>
                </th>
                <th align="center" valign="middle" >
                  B/B<sub>0</sub>
                </th>
                <th align="center" valign="middle" >
                  B<sub>MSY</sub>/B<sub>0</sub>
                </th>
                <th align="center" valign="middle" >
                  B/B<sub>MSY</sub>
                </th>
                <th align="center" valign="middle" >F/M</th>
              </tr>
            </thead>
            <tr>
              <td align="center" valign="middle" >17</td>
              <td align="center" valign="middle" >17</td>
              <td align="center" valign="middle" >1</td>
              <td align="center" valign="middle" >1</td>
              <td align="center" valign="middle" >0.91</td>
              <td align="center" valign="middle" >0.41</td>
              <td align="center" valign="middle" >0.34</td>
              <td align="center" valign="middle" >1.2</td>
              <td align="center" valign="middle" >0.81</td>
            </tr>
          </tbody>
        </table>
      </table-wrap>
      </sec> </body>
      <back>
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        </ref-list>
      </back>
</article>