<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">OJAS</journal-id><journal-title-group><journal-title>Open Journal of Animal Sciences</journal-title></journal-title-group><issn pub-type="epub">2161-7597</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/ojas.2024.141001</article-id><article-id pub-id-type="publisher-id">OJAS-129631</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biomedical&amp;Life Sciences</subject></subj-group></article-categories><title-group><article-title>
 
 
  Technical-Economic Performance, Carcass and Meat Quality of COBB500 Broilers Fed Rations Incorporating Mango By-Product Feeds in Ivory Coast (West Africa)
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Timbilfou</surname><given-names>Kiendrébéogo</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Alidjata</surname><given-names>Traoré</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Etienne</surname><given-names>Sodre</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Drissa</surname><given-names>Barry</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Isidor</surname><given-names>Ouedraogo</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Marcelle</surname><given-names>Grace Koidja</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Vianney</surname><given-names>Tarpaga</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib></contrib-group><aff id="aff5"><addr-line>Centre National de la Recherche Scientifique et Technologique, Institut de l’Environnement et de Recherches Agricoles, Laboratoire Central d’Horticulture (LCH), Bobo-Dioulasso, Burkina Faso</addr-line></aff><aff id="aff2"><addr-line>Université Catholique de l’Afrique de l’Ouest (UCAO) Section du Burkina Faso, Bobo-Dioulasso, Burkina Faso</addr-line></aff><aff id="aff4"><addr-line>Direction Régionale de l’Agriculture, des Ressources Animales et Halieutiques de l’Est, Fada, Burkina Faso</addr-line></aff><aff id="aff3"><addr-line>Centre National de la Recherche Scientifique et Technologique, Institut de l’Environnement et de Recherches Agricoles, Laboratoire de Recherches en Production et Santé Animales (LAREPSA), Bobo-Dioulasso, Burkina Faso</addr-line></aff><aff id="aff1"><addr-line>Laboratoire d’Etude des Ressources Naturelles et des Sciences de l’Environnement (LERNSE), Ecole Doctorale Sciences Naturelles et Agronomie (ED-SNA), Université Nazi BONI, Bobo-Dioulasso, Burkina Faso</addr-line></aff><pub-date pub-type="epub"><day>05</day><month>12</month><year>2023</year></pub-date><volume>14</volume><issue>01</issue><fpage>1</fpage><lpage>13</lpage><history><date date-type="received"><day>26,</day>	<month>October</month>	<year>2023</year></date><date date-type="rev-recd"><day>3,</day>	<month>December</month>	<year>2023</year>	</date><date date-type="accepted"><day>6,</day>	<month>December</month>	<year>2023</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
  The study evaluated the growth performance and carcass quality of COBB500 broilers fed two experimental rations incorporating feed and mango fine ED<sub>10</sub> for a batch of 83 birds, ED<sub>20</sub> (batch of 83 birds) in comparison with a commercial control ration CCD (84 birds). For this purpose, 250 day-old chicks of this breed were purchased in Korhogo and transported to Tengr&#233;la in Ivory Coast for rearing. The results show that substituting mango provender ans and mango seed flour for maize resulted in rations with costs per kgMS of 215.35FCFA, 2016.93FCFA and 320FCFA for the ED<sub>10</sub>, ED<sub>20</sub> and CCD rations respectively. The production costs per kg of chicken (FCPkgLW) of the CCD ration were significantly higher than those of the ED10 and ED<sub>20</sub> (p
   
  &lt;
   
  0
  .
  05). ADGs were significantly different and decreasing from CCD, ED<sub>20</sub> to ED<sub>10</sub> (p
   
  &lt;
   
  0.05). Average weights at 42
  nd
   day for ED<sub>20</sub> and CCD were homogeneous (p
   &gt; 
  0.05) and significantly higher than for ED
  <sub>10</sub>
   (p
   
  &lt;
   
  0
  .
  05). By day 49th the average weight of ED<sub>20</sub> had reached and exceeded that of CCD by day 42
  nd
  . PSLW, HCW
   
  and CCW were significantly different and decreasing from CCW, ED<sub>20</sub> to ED<sub>10</sub> (p
   
  &lt;
   
  0.05). Average hot (HCY) and cooled (CCY) carcass yields of ED<sub>20</sub> and CCD rations were comparable (
  p
   &gt; 
  0.05) and significantly higher than 
  those of ED<sub>10</sub>
   
  ration (p
   
  =
   
  0.009) and 0.003). Average drumstick and neck weights were significantly higher and lower for CCD, ED<sub>20</sub> and ED<sub>10</sub> (p
   
  &lt;
   
  0.05). For all other parts of the cut, the average weights of the CCD ration were significantly higher (p
   
  &lt;
   
  0.05) than those of the ED<sub>20</sub> and ED<sub>10</sub> rations, which were homogeneous (p
   
  &lt;
   
  0.05). The incorporation of mango provender and mango seed flour in broiler rations offers the prospect of reduced production costs, less competition between man and animals over cereals, and lean meat production that is more appreciated than that of chickens fed conventional feed. As it stands, the ED<sub>20</sub> ration can be recommended as a growth diet for broilers. It would be necessary to determine the levels of anti-nutritional substances in mango feeds and to assess the in vivo digestibility of mango ingredients and the diets incorporating them.
 
</p></abstract><kwd-group><kwd>Broiler</kwd><kwd> COOB 500 Chickens</kwd><kwd> Mango Feed</kwd><kwd> Carcass Characteristics</kwd><kwd> Tengrela</kwd><kwd> Ivory Coast</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>In African countries south of the Sahara, the need to substantially increase production in order to satisfy an ever-increasing demand for food is becoming an imperative. This need fits in well with the prospects of a 14% increase in global livestock production between 2020 and 2029 [<xref ref-type="bibr" rid="scirp.129631-ref1">1</xref>] . Within this dynamic, poultry remains the fastest-growing meat type, accounting for around half of the projected increase in total meat production. Many authors recognize that feed is the main constraint on livestock production in West Africa. In monogastric livestock production, particularly pigs and poultry, the food constraint is exacerbated by competition between animal species for the most widely used agricultural products (maize, millet, etc.) [<xref ref-type="bibr" rid="scirp.129631-ref2">2</xref>] . The cost of feed represents 55% - 60% of the production cost of poultry products, and the energy fraction represents 70% of the cost of feed [<xref ref-type="bibr" rid="scirp.129631-ref3">3</xref>] . Food savings can therefore make a substantial contribution to production efficiency. That’s why it is so important to use the good feed, at the lowest possible cost and in the right quantities, in the poultry ration. It is also important to know what feeds are available and how they should be used in poultry diets [<xref ref-type="bibr" rid="scirp.129631-ref1">1</xref>] . Also, non-conventional feeds are increasingly being used to reduce the financial burden of feed in production. Their use as partial or total substitutes for cereals reduces the competition between humans and animals for these conventional food sources. The possibility of producing feeds-based mango by-products [<xref ref-type="bibr" rid="scirp.129631-ref4">4</xref>] and use them in the rationing of monogastric pigs undergoing finishing growth [<xref ref-type="bibr" rid="scirp.129631-ref5">5</xref>] , laying chickens [<xref ref-type="bibr" rid="scirp.129631-ref6">6</xref>] and “bicycle” chickens [<xref ref-type="bibr" rid="scirp.129631-ref7">7</xref>] mixed-breed “Poulet du Faso” broiler chicken [<xref ref-type="bibr" rid="scirp.129631-ref8">8</xref>] has been successfully proven in Burkina Faso. On the strength of these results, a technology transfer agreement for the production of feed based on mango residues in Ivory Coast was draw up between the Fond Interprofessionnel pour la Recherche et le Conseil Agricoles (FIRCA) and the Institut de l’Environnement et de Recherche Agricole (INERA) in Burkina Faso. This is in view of the development and, above all, the processing of mangoes in Ivory Coast which are potential sources of production and processing residues that can be used as animal feed, including poultry feed. Indeed, despite the importance of the poultry sector, with its 44% contribution to meat production in Ivory Coast, this activity encounters nutritional and technical constraints, as seen from the angle of the frequent shortage of raw materials (maize, fish meal, cotton cake) and the poor quality of available feed [<xref ref-type="bibr" rid="scirp.129631-ref9">9</xref>] . The present study was carried out on the occasion of the transfer of mango by-product-based feed production technology. It also evaluated, for demonstration purposes, the effects of mango feed-based rations on growth parameters, carcass quality and organoleptic quality of COBB500 broiler meat in Ivory Coast.</p></sec><sec id="s2"><title>2. Materials and Methods</title><sec id="s2_1"><title>2.1. Study Site</title><p>The study was carried out on 2020 at Tengr&#233;la in Ivory Coast. Tengr&#233;la is a town located in the Savannas region in the extreme north of the country. Tengr&#233;la has a very dry, hot Sudanese climate. The vegetation is dominated by wooded savannah. The area produces mangoes and is ideal for livestock farming [<xref ref-type="bibr" rid="scirp.129631-ref10">10</xref>] .</p></sec><sec id="s2_2"><title>2.2. The Broiler Housing</title><p>The building used for the trial consists of a hangar topped with sheet metal and open on the west side. The other three sides are built of fixed masonry. It is subdivided into 3 pens of 10 m<sup>2</sup> each, each large enough to house a batch of 85 chickens from chick to sale. The screened enclosure provides good natural ventilation. It should also be noted that an electrical system was installed in the building to regulate temperature during the cool season. The flour of the henhouse was covered with litter at the recommended rate of 4.5 to 7.2 kg/m<sup>2</sup> depending on the raw material used [<xref ref-type="bibr" rid="scirp.129631-ref11">11</xref>] . The litter was changed every two weeks.</p></sec><sec id="s2_3"><title>2.3. Animal Materials</title><p>The animal material consisted of 255 COBB500 broiler chickens purchased at 01 day of age in Korhogo from Abidjan and transported to Tengr&#233;la. On arrival, the chickens were fed sugar water. A commercial starter feed was fed to all chicks without distinction for 01 week, before being replaced by the experimental rations once the batches had been formed. Three batches of 83, 83 and 84 chicks were formed, each receiving 01 control ration (CCD), an experimental ration with 10% mango seed floor (ED<sub>10</sub>) and a second experimental ration incorporating 20% mango fines (ED<sub>20</sub>).</p></sec><sec id="s2_4"><title>2.4. Formulation of Distributed Rations</title><p>Three rations, including two experimental ones (ED<sub>10</sub> and ED<sub>20</sub>), were formulated to be fed chickens per batch. These rations were formulated to reduce the incorporation rate of maize, the main source of energy in intensive and/or industrial rearing, by incorporating feed and mango seed floor. Their centesimal compositions and nutritional contributions are given in <xref ref-type="table" rid="table1">Table 1</xref>. The two experimental rations were compared with a control ration, which is a commercial broiler growth feed usually sold on the Ivorian market. Feeding was carried out in 2 phases. A start-up phase (from 1st to 10th day) during which the chicks were fed a starter diet purchased on the market, and a growth-finishing phase during which the chickens were fed ED<sub>10</sub>, ED<sub>20</sub> and RTC rations.</p></sec><sec id="s2_5"><title>2.5. Data Collection and Calculation of Analysis Parameters</title><p>➢ Zootechnical parameters: the zootechnical parameters studied concerned:</p><p>&#173; Weight evolution was assessed by weekly weighing of the chickens in the morning from 6 hours before the ration was served;</p><p>&#173; Growth rate was measured by Average Daily Gain (ADG) calculated according to the formula: ADG (g) = FW − IW where PF (g) = final weight and PI (g) = initial weight;</p><p>&#173; Daily food consumption (DFC) is calculated using the formula DFC (g) = (QFD − RF) where QDF (g) = amount of feed distributed the previous day and RF (g) = feed refusal measured the following day;</p><p>&#173; The consumption index (CI) was calculated using the formula: CI = DFC (g)/(ADG) (g)</p><p>➢ Carcass parameters: To assess carcass characteristics, chickens were first fasted for 24 hours and weighed before slaughter. The chickens were slaughtered and the carcasses cut according to the methods recommended by [<xref ref-type="bibr" rid="scirp.129631-ref12">12</xref>] . After plucking, warm carcasses were weighed and then chilled at 5˚C for 24 hours. The chilled carcasses were then weighed. Each piece of the cut and each organ were weighed. The data collected from the weighting’s were used to calculate:</p><table-wrap id="table1" ><label><xref ref-type="table" rid="table1">Table 1</xref></label><caption><title> Centesimal composition of the distributed diets</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Ingredients</th><th align="center" valign="middle"  colspan="3"  >Distributed diets</th></tr></thead><tr><td align="center" valign="middle" >ED<sub>10</sub></td><td align="center" valign="middle" >ED<sub>20</sub></td><td align="center" valign="middle" >CCD</td></tr><tr><td align="center" valign="middle" >Corn</td><td align="center" valign="middle" >35</td><td align="center" valign="middle" >33</td><td align="center" valign="middle" >-</td></tr><tr><td align="center" valign="middle" >Mango food</td><td align="center" valign="middle" >26.75</td><td align="center" valign="middle" >25</td><td align="center" valign="middle" >0</td></tr><tr><td align="center" valign="middle" >Mango almond</td><td align="center" valign="middle" >10</td><td align="center" valign="middle" >20</td><td align="center" valign="middle" >0</td></tr><tr><td align="center" valign="middle" >Vegetable oil</td><td align="center" valign="middle" >2</td><td align="center" valign="middle" >0</td><td align="center" valign="middle" >-</td></tr><tr><td align="center" valign="middle" >Fish</td><td align="center" valign="middle" >7</td><td align="center" valign="middle" >2.75</td><td align="center" valign="middle" >-</td></tr><tr><td align="center" valign="middle" >KLC</td><td align="center" valign="middle" >19.25</td><td align="center" valign="middle" >19.25</td><td align="center" valign="middle" >-</td></tr><tr><td align="center" valign="middle" >Total</td><td align="center" valign="middle" >100</td><td align="center" valign="middle" >100</td><td align="center" valign="middle" >-</td></tr></tbody></table></table-wrap><p>ED<sub>10</sub> = experimental ration incorporating mango by-products including 10% mango seed flour; ED<sub>20</sub> = experimental Ration incorporating mango by-products including 20% mango seed floor; CCD = commercial control ration without mango by-products.</p><p>&#173; Hot carcass yield (HCY) according to the formula: HCY (%) = (HCW/PSLW) &#215; 100 were HCW = hot carcass weight and PSLW = pre-slaughter live weight;</p><p>&#173; Cold carcass yield (CCY) according to the formula: CCY (%) = (CCW/PSLW) &#215; 100 were CCW = cold carcass weight and PSLW = pre-slaughter live weight;</p><p>&#173; Abdominal fat rate (AF) according to the formula: AF (%) = (AFW/CCW) &#215; 100 were AFW = total weight of abdominal fat and CCW = cold carcass weight;</p><p>➢ Organoleptic quality of the meat: organoleptic qualities were determined by evaluating the tenderness and succulence of the meat, by tasting the salted meat fried in oil. A panel of twenty-five (25) people (men and women) carried out the tasting. The meat was served in two dishes, one identified by pink wallpaper and the other blue, containing respectively meat from the CCD ration and meat from the ED<sub>10</sub> and ED<sub>20</sub> rations. It should be noted that only the organizers could differentiate between the meats served. The carcasses were cut to ensure uniformity in the size of the pieces of meat served. After tasting, we recorded each participant’s appreciation in an event notebook for analysis.</p><p>The data collected were entered using Excel 2016 software, and the analysis was carried out with XLSTAT 2016 software using an ANOVA for the separation of means at the 5% threshold by Fisher LSD test.</p></sec></sec><sec id="s3"><title>3. Results</title><sec id="s3_1"><title>3.1. Influence of Mango Feed Incorporation on Ration Characteristics and Costs</title><p>The energy and nutritional composition of the rations is shown in <xref ref-type="table" rid="table2">Table 2</xref>. The levels of incorporation of mango seed floor in ED<sub>10</sub> (10%) and ED<sub>20</sub> (20%) rations resulted in a higher level of fish incorporation in the experimental diets ED<sub>10</sub> which were higher than those ED<sub>20</sub>. The ED<sub>10</sub> and ED<sub>20</sub> rations were iso-energetic and iso-proteinic. The Crude Fiber (CF) contents of the RD<sub>10</sub> and ED<sub>20</sub> rations were virtually equal and higher than those of the commercial CD ration. The cost per kg of the ED<sub>10</sub> and ED<sub>20</sub> rations was lower than that of the CD ration.</p></sec><sec id="s3_2"><title>3.2. Weight Trends during the Growth Phase</title><p><xref ref-type="fig" rid="fig1">Figure 1</xref> shows that growth was continuous for all three rations from D1 to D35. The results in <xref ref-type="table" rid="table3">Table 3</xref> show that, apart from the weights at D1 and D7, all other average weekly weights were significantly different (p &lt; 0.05). From D7 to D35, the CCD ration was on average significantly higher than the ED<sub>10</sub> ration, which was in turn significantly higher than ED<sub>20</sub> (p = 0.000). At the seventh weighing (D49), the average weight of ED<sub>20</sub> was significantly higher (p = 0.000) than those of CCD and ED<sub>10</sub>, which were homogeneous (p = 0.000).</p><table-wrap id="table2" ><label><xref ref-type="table" rid="table2">Table 2</xref></label><caption><title> Feed values of rations distributed</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Ingredients (%)</th><th align="center" valign="middle"  colspan="3"  >Rations distributed</th></tr></thead><tr><td align="center" valign="middle" >ED<sub>10</sub></td><td align="center" valign="middle" >ED<sub>20</sub></td><td align="center" valign="middle" >CCD</td></tr><tr><td align="center" valign="middle" >ED (kcal/kg)</td><td align="center" valign="middle" >2906</td><td align="center" valign="middle" >2925</td><td align="center" valign="middle" >3146</td></tr><tr><td align="center" valign="middle" >Protein (%)</td><td align="center" valign="middle" >17.13</td><td align="center" valign="middle" >17.26</td><td align="center" valign="middle" >18.99</td></tr><tr><td align="center" valign="middle" >Lysine (%)</td><td align="center" valign="middle" >1.41</td><td align="center" valign="middle" >1.51</td><td align="center" valign="middle" >-</td></tr><tr><td align="center" valign="middle" >Methionine (%)</td><td align="center" valign="middle" >0.67</td><td align="center" valign="middle" >0.75</td><td align="center" valign="middle" >-</td></tr><tr><td align="center" valign="middle" >Calcium (%)</td><td align="center" valign="middle" >1.5</td><td align="center" valign="middle" >2.67</td><td align="center" valign="middle" >8.3</td></tr><tr><td align="center" valign="middle" >Phosphorus (%)</td><td align="center" valign="middle" >0.37</td><td align="center" valign="middle" >0.27</td><td align="center" valign="middle" >6.25</td></tr><tr><td align="center" valign="middle" >CB (%)</td><td align="center" valign="middle" >10.57</td><td align="center" valign="middle" >10.06</td><td align="center" valign="middle" >5.12</td></tr><tr><td align="center" valign="middle" >Costs (FCFA/kgMS)</td><td align="center" valign="middle" >215.35</td><td align="center" valign="middle" >216.93</td><td align="center" valign="middle" >320</td></tr></tbody></table></table-wrap><p>ED<sub>10</sub> = experimental ration 1 incorporating mango by-products including 10% mango kernel; ED<sub>20</sub> = experimental; Ration incorporating mango by-products including 20% mango kernel; CCD = commercial control ration without mango by-products.</p><table-wrap id="table3" ><label><xref ref-type="table" rid="table3">Table 3</xref></label><caption><title> Average weekly chicken weights according to rations</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Period</th><th align="center" valign="middle"  colspan="3"  >Diets distributed</th><th align="center" valign="middle"  rowspan="2"  >Pr ˃ F</th><th align="center" valign="middle"  rowspan="2"  >Sig.</th></tr></thead><tr><td align="center" valign="middle" >ED<sub>10</sub></td><td align="center" valign="middle" >ED<sub>20</sub></td><td align="center" valign="middle" >CCD</td></tr><tr><td align="center" valign="middle" >W0</td><td align="center" valign="middle" >148 &#177; 30<sup>a</sup></td><td align="center" valign="middle" >150 &#177; 35<sup>a</sup></td><td align="center" valign="middle" >152 &#177; 29<sup>a</sup></td><td align="center" valign="middle" >0.806</td><td align="center" valign="middle" >No</td></tr><tr><td align="center" valign="middle" >W1</td><td align="center" valign="middle" >211 &#177; 45<sup>a</sup></td><td align="center" valign="middle" >231 &#177; 54<sup>a</sup></td><td align="center" valign="middle" >223 &#177; 51<sup>a</sup></td><td align="center" valign="middle" >0.183</td><td align="center" valign="middle" >No</td></tr><tr><td align="center" valign="middle" >W2</td><td align="center" valign="middle" >282 &#177; 51<sup>a</sup></td><td align="center" valign="middle" >327 &#177; 70<sup>b</sup></td><td align="center" valign="middle" >445 &#177; 111<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >W3</td><td align="center" valign="middle" >351 &#177; 62<sup>a</sup></td><td align="center" valign="middle" >390 &#177; 76<sup>b</sup></td><td align="center" valign="middle" >618 &#177; 150<sup>b</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >W4</td><td align="center" valign="middle" >476 &#177; 72<sup>a</sup></td><td align="center" valign="middle" >582 &#177; 130<sup>b</sup></td><td align="center" valign="middle" >929 &#177; 206<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >W5</td><td align="center" valign="middle" >646 &#177; 103<sup>a</sup></td><td align="center" valign="middle" >781 &#177; 191<sup>b</sup></td><td align="center" valign="middle" >1308 &#177; 251<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >W6</td><td align="center" valign="middle" >1081 &#177; 163<sup>a</sup></td><td align="center" valign="middle" >1080 &#177; 189<sup>b</sup></td><td align="center" valign="middle" >1308 &#177; 251<sup>b</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >W7</td><td align="center" valign="middle" >1261 &#177; 140<sup>b</sup></td><td align="center" valign="middle" >1510 &#177; 180<sup>a</sup></td><td align="center" valign="middle" >-</td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr></tbody></table></table-wrap><p>ED<sub>10</sub> = experimental ration incorporating 10% of mango seed flour; ED<sub>20</sub>: experimental ration incorporating 20% of mango seed floor; CCD = commercial control ration without mango. Averages on the same line bearing the same letter are not significantly different at the significance levels indicated.</p></sec><sec id="s3_3"><title>3.3. Growth, Conversion and Production Costs for Live Chickens</title><p>The results in <xref ref-type="table" rid="table4">Table 4</xref> shows that ADG were significantly different and decreasing from CCD, ED<sub>20</sub> to ED<sub>10</sub> (p = 0.000). No significant difference was observed in average daily feed intake between diets. The CI for the ED<sub>10</sub> ration was significantly higher than that for CCD (p = 0.039). The average FCkgLW of the ED<sub>10</sub> and ED<sub>20</sub> experimental rations was significantly lower than that of the CCD ration (p = 0.027).</p></sec><sec id="s3_4"><title>3.4. Characteristics of Broiler Carcasses</title><p>The results in <xref ref-type="table" rid="table5">Table 5</xref> shows that average pre-slaughter live weights (PSLW), hot carcass weights (HCW) and cooled carcass weights (CCW) were significantly higher and decreased from CCD, ED<sub>20</sub> to ED<sub>10</sub> rations (p = 0.000). The average</p><table-wrap id="table4" ><label><xref ref-type="table" rid="table4">Table 4</xref></label><caption><title> Growth (ADG) and conversion index (CI) parameters and feed cost of 01 kg of live weight of chicken (FCkgLW)</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Parameters</th><th align="center" valign="middle"  colspan="3"  >Diets distributed</th><th align="center" valign="middle"  rowspan="2"  >Pr ˃ F</th><th align="center" valign="middle"  rowspan="2"  >Sig.</th></tr></thead><tr><td align="center" valign="middle" >ED<sub>10</sub> (n = 43)</td><td align="center" valign="middle" >ED<sub>20</sub> (n = 43)</td><td align="center" valign="middle" >CCD (n = 84)</td></tr><tr><td align="center" valign="middle" >DFC</td><td align="center" valign="middle" >71.07 &#177; 17.57</td><td align="center" valign="middle" >71.95 &#177; 19.11</td><td align="center" valign="middle" >71.47 &#177; 19.03</td><td align="center" valign="middle" >0.976</td><td align="center" valign="middle" >No</td></tr><tr><td align="center" valign="middle" >ADG</td><td align="center" valign="middle" >22.72 &#177; 2.96<sup>c</sup></td><td align="center" valign="middle" >27.75 &#177; 3.69<sup>b</sup></td><td align="center" valign="middle" >33.03 &#177; 6.95<sup>a</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >CI</td><td align="center" valign="middle" >3.44.56<sup>a</sup></td><td align="center" valign="middle" >2.82 &#177; 0.54<sup>ab</sup></td><td align="center" valign="middle" >2.69 &#177; 2.14<sup>b</sup></td><td align="center" valign="middle" >0.039</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >FCPkgLW</td><td align="center" valign="middle" >739 &#177; 121<sup>ab</sup></td><td align="center" valign="middle" >612 &#177; 117<sup>b</sup></td><td align="center" valign="middle" >859 &#177; 686<sup>a</sup></td><td align="center" valign="middle" >0.027</td><td align="center" valign="middle" >Yes</td></tr></tbody></table></table-wrap><p>ADG = average daily gain; CI = conversion index; FCPkgLW = feed cost of 01 kg chicken live weight production; DFC = daily feed consumption per subject; ED<sub>10</sub> = experimental ration with 10% mango seed flour; ED<sub>20</sub>: experimental ration with 20% mango seed floor; CCD = commercial control ration without mango. Average means in the same line bearing the same letter are not significantly different at the significance levels in the table.</p><table-wrap id="table5" ><label><xref ref-type="table" rid="table5">Table 5</xref></label><caption><title> Comparative average characteristics of broiler carcasses</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Parameters</th><th align="center" valign="middle"  colspan="3"  >Diets distributed</th><th align="center" valign="middle"  rowspan="2"  >Pr ˃ F</th><th align="center" valign="middle"  rowspan="2"  >Sig.</th></tr></thead><tr><td align="center" valign="middle" >ED<sub>10</sub> (n = 43)</td><td align="center" valign="middle" >ED<sub>20</sub> (n = 43)</td><td align="center" valign="middle" >CCD (n = 84)</td></tr><tr><td align="center" valign="middle" >PSLW (g)</td><td align="center" valign="middle" >1013 &#177; 255<sup>a</sup></td><td align="center" valign="middle" >1206 &#177; 116<sup>b</sup></td><td align="center" valign="middle" >1707 &#177; 159<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >HCW (g)</td><td align="center" valign="middle" >712 &#177; 194<sup>a</sup></td><td align="center" valign="middle" >921 &#177; 105<sup>b</sup></td><td align="center" valign="middle" >1286 &#177; 127<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >CCW (g)</td><td align="center" valign="middle" >616 &#177; 178<sup>a</sup></td><td align="center" valign="middle" >829 &#177; 129<sup>b</sup></td><td align="center" valign="middle" >1176 &#177; 78<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >HCY (%)</td><td align="center" valign="middle" >70 &#177; 4<sup>a</sup></td><td align="center" valign="middle" >76 &#177; 6<sup>b</sup></td><td align="center" valign="middle" >75 &#177; 3<sup>b</sup></td><td align="center" valign="middle" >0.009</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >CCY (%)</td><td align="center" valign="middle" >60 &#177; 4<sup>a</sup></td><td align="center" valign="middle" >69 &#177; 7<sup>b</sup></td><td align="center" valign="middle" >69 &#177; 6<sup>b</sup></td><td align="center" valign="middle" >0.003</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >AF (%)</td><td align="center" valign="middle" >3 &#177; 1<sup>a</sup></td><td align="center" valign="middle" >2 &#177; 1<sup>b</sup></td><td align="center" valign="middle" >2 &#177; 0.4<sup>b</sup></td><td align="center" valign="middle" >0.004</td><td align="center" valign="middle" >Yes</td></tr></tbody></table></table-wrap><p>ED<sub>10</sub> = experimental ration with 10% mango seed flour; ED<sub>20</sub>: experimental ration with 20% mango seed floor; CCD = commercial control ration without mango. PSLW = pre-slaughter live weight of chicken, HCW = hot carcass weight, CCW = weight of chicken after slaughter, HCY = hot carcass yield, CCY = cold carcass yield. Average means in the same line bearing the same letter are not significantly different at the significance levels in the table.</p><p>hot carcass yield (HCY) and cold carcass yields (CCY) of rations ED<sub>20</sub> and CCD were comparable (p &gt; 0.05) and significantly higher than those of ration ED<sub>10</sub> (p = 0.009 and p = 0.003). Conversely, the mean abdominal fat content (AF) of ED<sub>10</sub> was significantly higher than that of rations ED<sub>20</sub> and CCD (p = 0.004), which were comparable with each other (p &gt; 0.05).</p></sec><sec id="s3_5"><title>3.5. Characteristics of Carcass Cuts</title><p>Our results for carcass cuts (<xref ref-type="table" rid="table6">Table 6</xref>) show that:</p><p>- The average weights of the whole thigh, wings, chest and back of the CCD were significantly higher than those of the ED<sub>10</sub> and ED<sub>20</sub> rations (p = 0.000), which were comparable to each other;</p><p>- Mean drumstick and neck weights were significantly different and decreased from CCD, ED<sub>20</sub> to ED<sub>10</sub> (p = 0.000).</p></sec><sec id="s3_6"><title>3.6. Organs’ Characteristics</title><p>The results presented in <xref ref-type="table" rid="table7">Table 7</xref> shows the offal and organ weights of COOB 500 broilers after slaughter, according to ration. Average total offal and gizzard weights of the RT ration were significantly higher than those of the ED<sub>10</sub> (p = 0.000) and ED<sub>20</sub> (p = 0.001) rations, which were homogeneous between them. Average gutted gizzard weights of RT and ED<sub>20</sub> were comparable and significantly higher than those of the ED<sub>10</sub> ration (p = 0.004). Average leg and head weights were significantly different and decreased from RT and ED<sub>20</sub> to ED<sub>10</sub> (p = 0.000).</p></sec><sec id="s3_7"><title>3.7. Organoleptic Quality of Broiler Meat</title><p>The results of the taste test showed that the tenderness of the meat from chickens on the mango diet was tenderer for 60% of consumers, compared with</p><table-wrap id="table6" ><label><xref ref-type="table" rid="table6">Table 6</xref></label><caption><title> Characteristics of carcass cuts</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Parameters</th><th align="center" valign="middle"  colspan="3"  >Diets distributed</th><th align="center" valign="middle"  rowspan="2"  >Pr ˃ F</th><th align="center" valign="middle"  rowspan="2"  >Sig.</th></tr></thead><tr><td align="center" valign="middle" >ED<sub>10</sub> (n = 43)</td><td align="center" valign="middle" >ED<sub>20</sub> (n = 43)</td><td align="center" valign="middle" >CCD (n = 84)</td></tr><tr><td align="center" valign="middle" >Whole legs</td><td align="center" valign="middle" >361.5<sup>a</sup></td><td align="center" valign="middle" >254.5<sup>b</sup></td><td align="center" valign="middle" >206.5<sup>b</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Pestle (g)</td><td align="center" valign="middle" >163.5<sup>a</sup></td><td align="center" valign="middle" >117.5<sup>b</sup></td><td align="center" valign="middle" >90.5<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Thigh (g)</td><td align="center" valign="middle" >196.5<sup>a</sup></td><td align="center" valign="middle" >134<sup>b</sup></td><td align="center" valign="middle" >114<sup>b</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Wing (g)</td><td align="center" valign="middle" >146<sup>a</sup></td><td align="center" valign="middle" >99<sup>b</sup></td><td align="center" valign="middle" >80<sup>b</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Neck (g)</td><td align="center" valign="middle" >60<sup>a</sup></td><td align="center" valign="middle" >50<sup>b</sup></td><td align="center" valign="middle" >39.5<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Breast (g)</td><td align="center" valign="middle" >331<sup>a</sup></td><td align="center" valign="middle" >245<sup>b</sup></td><td align="center" valign="middle" >183.5<sup>b</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Dos (g)</td><td align="center" valign="middle" >222.5<sup>a</sup></td><td align="center" valign="middle" >159<sup>b</sup></td><td align="center" valign="middle" >134.5<sup>b</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr></tbody></table></table-wrap><p>ED<sub>10</sub> = experimental ration with 10% mango seed floor; ED<sub>20</sub>: experimental ration with 20% mango seed floor; CCD = commercial control ration without mango; Average means in the same line bearing the same letter are not significantly different at the significance levels in the table.</p><table-wrap id="table7" ><label><xref ref-type="table" rid="table7">Table 7</xref></label><caption><title> Offal and organ weights for COBB500 broilers</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Parameters</th><th align="center" valign="middle"  colspan="3"  >Distributed diets</th><th align="center" valign="middle"  rowspan="2"  >Pr ˃ F</th><th align="center" valign="middle"  rowspan="2"  >Sig.</th></tr></thead><tr><td align="center" valign="middle" >ED<sub>10</sub> (n = 43)</td><td align="center" valign="middle" >ED<sub>20</sub> (n = 43)</td><td align="center" valign="middle" >CCD (n = 84)</td></tr><tr><td align="center" valign="middle" >Total offal (g)</td><td align="center" valign="middle" >93.5<sup>a</sup></td><td align="center" valign="middle" >73<sup>b</sup></td><td align="center" valign="middle" >67.5<sup>b</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Full gizzard (g)</td><td align="center" valign="middle" >56<sup>a</sup></td><td align="center" valign="middle" >41.5<sup>b</sup></td><td align="center" valign="middle" >39<sup>b</sup></td><td align="center" valign="middle" >0.001</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Empty gizzard (g)</td><td align="center" valign="middle" >32<sup>a</sup></td><td align="center" valign="middle" >29.5<sup>a</sup></td><td align="center" valign="middle" >23<sup>b</sup></td><td align="center" valign="middle" >0.004</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Liver (g)</td><td align="center" valign="middle" >26.5<sup>a</sup></td><td align="center" valign="middle" >25<sup>a</sup></td><td align="center" valign="middle" >22.5<sup>a</sup></td><td align="center" valign="middle" >0.275</td><td align="center" valign="middle" >No</td></tr><tr><td align="center" valign="middle" >Heart (g)</td><td align="center" valign="middle" >8<sup>a</sup></td><td align="center" valign="middle" >7.5<sup>a</sup></td><td align="center" valign="middle" >6.5<sup>a</sup></td><td align="center" valign="middle" >0.418</td><td align="center" valign="middle" >No</td></tr><tr><td align="center" valign="middle" >Leg (g)</td><td align="center" valign="middle" >77.5<sup>a</sup></td><td align="center" valign="middle" >60<sup>b</sup></td><td align="center" valign="middle" >40.5<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr><tr><td align="center" valign="middle" >Head (g)</td><td align="center" valign="middle" >49.5<sup>a</sup></td><td align="center" valign="middle" >40.5<sup>b</sup></td><td align="center" valign="middle" >34.5<sup>c</sup></td><td align="center" valign="middle" >0.000</td><td align="center" valign="middle" >Yes</td></tr></tbody></table></table-wrap><p>ED<sub>10</sub> = experimental ration with 10% of mango seed flour; ED<sub>20</sub>: experimental ration with 20% of mango seed flour; CCD = commercial control ration without mango; Average means in the same line bearing the same letter are not significantly different at the significance levels in the table.</p><p>40% on the CCD diet. The succulence of meat from diets incorporating mango was also judged more succulent by 76% of consumers, compared with 24% for the CCD diet.</p></sec></sec><sec id="s4"><title>4. Discussion</title><sec id="s4_1"><title>4.1. Effects of Rations Incorporating Feed and Mango Fines on the Technical and Economic Growth Performance of Broilers</title><p>Our results on broiler weight development showed continuous growth from the beginning to the end of the trial. However, the growth rate of the commercial control ration (CCD) was higher than that of the ED<sub>10</sub> and ED<sub>20</sub> experimental rations. These results confirm our expectations on these two parameters. They corroborate results reported by other authors such as [<xref ref-type="bibr" rid="scirp.129631-ref6">6</xref>] and [<xref ref-type="bibr" rid="scirp.129631-ref7">7</xref>] who tested rations based on mango by-products respectively in the feed of growing Isa Brown layers and growing-finishing local breed chickens respectively. Since mango by-products are rich in fiber, they have certainly had an upward influence on the fiber content of rations, which is not conducive to good digestibility and therefore good availability of nutrients, especially energy. The consequence of this situation is that after 42 days of rearing, the CCD subjects had reached an average weight (1308 &#177; 251 g) acceptable for sale. On the other hand, ED<sub>10</sub> and ED<sub>20</sub> subjects waited a week longer to reach average weights of over 1200 g. At 42 days of age, the average weights of all our ED<sub>10</sub> (1081 &#177; 16 g), ED<sub>20</sub> (1080 &#177; 19 g) and CCD (1308 &#177; 25 g) rations are lower than those reported by [<xref ref-type="bibr" rid="scirp.129631-ref13">13</xref>] for 2 experimental rations based on maize espoir variety (1743.8 &#177; 248.0 g) and SR21 (1721.3 &#177; 250.2 g) compared with a commercial ration (1610.3 &#177; 244.9 g). In addition, the CI of the ED<sub>20</sub> ration was significantly comparable to that of the CCD, meaning that these two rations were the most highly valued compared with the experimental ED<sub>10</sub> ration. These results show that the mango by-product feeds (mango meal and mango seed flour) used in the ED<sub>10 </sub>(36.75%) and ED<sub>20</sub> (45%) rations can be a good substitute for maize, which is recognized as a major source of energy. The effects of rationing trials based on maize (60.23%) as the main source of energy and groundnut meal (25.49%) as the main source of protein [<xref ref-type="bibr" rid="scirp.129631-ref14">14</xref>] showed the same growth rate for control rations in both cases, and double that of our experimental rations. However, the conversion levels (CI) measured weekly in the trial were close to those of our ED<sub>20</sub> ration. This performance of the ED<sub>20</sub>, ration, which also doubled its weight gain compared with the ED<sub>10</sub> ration between W6 and W7, could be explained by the influence of the additional rate (10%) of incorporation of mango seed flour Contrary to weight performance, feed production costs of per kg live chicken (FCPkgLW) were lower for the ED<sub>10</sub> and ED<sub>20</sub> rations than for the CCD ration.</p></sec><sec id="s4_2"><title>4.2. Influence of Rations Incorporating Mango Provender and Mango Seed Flour on Carcass Characteristics and Organoleptic Quality of Meat</title><p>Our results on the average characteristics of broiler carcasses (<xref ref-type="table" rid="table5">Table 5</xref>) show the same levels of abdominal fat rate (AF) between the ED<sub>20</sub> and CCD rations, which are significantly lower than that of ED<sub>10</sub>. The opposite is true for carcass yields (HCY and CCY), which are comparable for the ED<sub>20</sub> and CCD rations, but significantly higher than that of ED<sub>10</sub>. These results run counter to the average of PSLW, HCW and CCW, which are significantly higher and decreasing for CCD, ED<sub>20</sub> and ED<sub>10</sub> rations. This demonstrates the ability of the ED<sub>20</sub> ration to produce more meat than the ED<sub>10</sub> ration and as much lean meat as the CCD ration. In monogastric animals, fibrous feeds favor the production of lean meat, which in principle weighs more than fatty meat. The evaluation of mango-fed pig carcasses by [<xref ref-type="bibr" rid="scirp.129631-ref15">15</xref>] and [<xref ref-type="bibr" rid="scirp.129631-ref5">5</xref>] demonstrated this assertion. Note that according to [<xref ref-type="bibr" rid="scirp.129631-ref16">16</xref>] mango kernels have antioxidant properties that reduce fat formation in carcasses. Our results obtained for the CCD and ED<sub>20</sub> are very close to the abdominal fat levels reported by [<xref ref-type="bibr" rid="scirp.129631-ref16">16</xref>] for broilers fed increasing levels of caterpillar meal and fish meal, for which it had been concluded that they were capable of producing lean meat. The significant difference in live weight at slaughter (LWS) depends on the final weights, which differed between the 3 rations. However, we note that the ED<sub>20</sub> has the same carcass yield as the CCD. As the aim of rearing chickens is to gain meat, the almost equal yields between the CCD and the ED<sub>20</sub> show that the latter, based on mango by-products, makes up for the chickens’ growth deficit through meat yield. This may be explained by the higher fat content of CCD carcasses compared with experimental rations. If we add to these good yield performances the lower production feed cost of the ED<sub>20</sub> ration than that of the CCD, we can conclude that it is technically and economically competitive with the control ration.</p><p>Meat from rations incorporating mango feed received the highest ratings for both tenderness and succulence, compared with that from the CCD mango-free control diet. This shows that, in addition to good carcass yield, the mango-based ration provides lean, tender and succulent meat, qualities that most consumers are looking for. If we add to these good organoleptic performances, good yields and low production costs, we can encourage the use of feed based on eating byproducts in broiler diets.</p></sec></sec><sec id="s5"><title>5. Conclusion</title><p>At the end of this study, it emerged that the incorporation of feed and mango kernels in broiler rations offers an interesting prospect in terms of production feed costs and carcass quality. The results of the experiment show that good carcass performance and appreciable organoleptic qualities make it technically and economically possible to make up for the growth lag experienced in the ED<sub>20</sub> experimental ration. These results show that feed and mango kernel can partially replace cereals and other ingredients in broiler rations. This not only limits competition between man and beast in the use of cereals, but also makes it possible to use large quantities of mango by-products, which used to be bulky and thrown away in nature. As it stands, the ED<sub>20</sub> ration can be recommended as a growth diet for broilers. In the future, it will be necessary to determine the exact composition of mango kernels for better use in modern poultry feed, and also for other animal species. Also, to encourage the transfer of technology to other mango-producing countries, and to promote the technology among mango processors and breeders in order to increase their income.</p></sec><sec id="s6"><title>Acknowledgements</title><p>We would like to thank the Fond Interprofessionnel pour la Recherche et le Conseils Agricoles (FIRCA) for funding the technology transfer to C&#244;te d’Ivoire, and the Institut de l’Environnement et de Recherches Agricoles (INERA) of CNRST (Burkina Faso) for facilitating it by sending a team of experts for the successful implementation of the activities.</p></sec><sec id="s7"><title>Conflicts of Interest</title><p>The authors declare no conflicts of interest regarding the publication of this paper.</p></sec><sec id="s8"><title>Cite this paper</title><p>Kiendr&#233;b&#233;ogo, T., Traor&#233;, A., Sodre, E., Barry, D., Ouedraogo, I., Koidja, M.G. and Tarpaga, V. (2024) Technical-Economic Performance, Carcass and Meat Quality of COBB500 Broilers Fed Rations Incorporating Mango By-Product Feeds in Ivory Coast (West Africa). Open Journal of Animal Sciences, 14, 1-13. https://doi.org/10.4236/ojas.2024.141001</p></sec></body><back><ref-list><title>References</title><ref id="scirp.129631-ref1"><label>1</label><mixed-citation publication-type="other" xlink:type="simple">FAO (2020) Rising Uncertainties from COVID-19 Cloud Medium-Term Agricultural Prospects. OECD-FAO Agricultural Outlook 2020-2029. https://www.fao.org/news/story/en/item/1298333/icode/</mixed-citation></ref><ref id="scirp.129631-ref2"><label>2</label><mixed-citation publication-type="other" xlink:type="simple">Zagr&amp;#233, H., Kiendr&amp;#233b&amp;#233ogo, T., Ou&amp;#233draogo, S., Barry, D., Ou&amp;#233draogo, I.Z., Mopat&amp;#233, Y.L. and Kabor&amp;#233 Zoungrana, C.-Y. 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