JBMJournal of Biosciences and Medicines2327-5081Scientific Research Publishing10.4236/jbm.2023.1111009JBM-128990ArticlesBiomedical&Life Sciences Relationship between the Polymorphism of the <i>GSTP1</i> (rs1695) Gene and Chronic Hepatitis B Infection in Ouagadougou, Burkina Faso TilateLare1LassinaTraore1MarieSimone Traore2SidnoomaVéronique Zongo1PierreZabre1MoussoSavadogo1FortuneD. Salah3HermanKarim Sombie4PegdwendéAbel Sorgho4TégwindéRebeca Compaore5TaniSagna5IssoufouTao6FlorenciaWendkuuni Djigma1*DorcasObiri-Yeboah7DamintotiSimplice Karou8RogomenomaAlice Ouedraogo9Teega-WendeClarisse Ouedraogo1ProsperBado4AlbertThéophane Yonli4JacquesSimpore4Centre Universitaire de Gaoua, Université Nazi Boni, Bobo-Dioulasso, Burkina FasoInstitut des Sciences et de Technologies/Ecole Normale Supérieure, Koudougou, Burkina FasoCentre National de la Recherche Scientifique et Technologique, Institut de Recherche en Sciences de la Santé, Ouagadougou, Burkina FasoLaboratoire de Biologie Moléculaire-Virologie, Institut National d’Hygiène, Lomé, TogoCentre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina FasoLaboratoire de Biologie Moléculaire et de Génétique, Université Joseph KI-ZERBO, Ouagadougou, Burkina FasoDepartment of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, PMB, Cape Coast, Ghanaécole Supérieure des Techniques Biologiques et Alimentaires, Université de Lomé (ESTBA-UL), Lomé, TogoEcole Normale Supérieure, Ouagadougou, Burkina Faso0111202311119410711, September 20237, November 2023 10, November 2023© Copyright 2014 by authors and Scientific Research Publishing Inc. 2014This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/

Introduction: Genetic polymorphisms of some Glutathione S-Transferase (GST) which encode the enzyme responsible for the biotransformation of drugs and xenobiotics, have been associated with the risk of several pathologies that can progress to cancer such as Hepatitis B. This study aims to characterize the impact of the rs1695 polymorphism of GSTP1 gene among people with chronic Hepatitis B infection in Burkina Faso. Methods: rs1695 polymorphisms of GSTP1 gene genotyping was performed for 50 people infected with chronic Hepatitis B virus and 124 healthy people with the PCR-RFLP method. Conventional PCR was used for DNA amplification and Alw26I enzyme was used for enzymatic digestion. Results: The results show that the frequencies of AA, AG and GG genotypes are respectively 31.00%, 36.80% and 32.20% in general the study population with a mutation rate of 50.57%. However, the incidence of the AA, AG and GG genotypes are respectively 30.64%, 38.71% and 30.64% among people with chronic Hepatitis B virus infection and 32.00%, 32.00% and 36.00% among healthy people. In cases, the frequencies of the A and G alleles are 48.00% and 52.00% respectively, and in controls 50.00% each. No statistical difference was found by comparing genotypic and allelic frequencies between cases and controls (p > 0.05). Conclusion: Our study allowed us to determine the rate of GSTP1 rs1695 genotypes in the study population, cases and controls. From our analyses, GSTP1 rs1695 is not associated to chronic Hepatitis B virus infection in Ouagadougou.

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