Malaria is a febrile erythrocytopathy caused by a haematozoan parasite of the genus Plasmodium. It is transmitted by the infecting bite of a mosquito called the female Anopheles. There are two forms of malaria: uncomplicated malaria, which generally progresses well under treatment, and severe malaria, which is fatal if not treated early and appropriately [1] . Malaria is a public health priority. It is the most widespread transmissible infectious disease in the world. It is responsible for very high morbidity and mortality, especially in low- and middle-income countries, notably in Sub-Saharan Africa, South-East Asia and South America. According to the WHO 2022 malaria report, the number of cases worldwide rose between 2020 and 2021. It was estimated at 247 million in 2021, compared with 245 million in 2020. The number of deaths rose from 625,000 in 2020 to 619,000 in 2021 [2] . The WHO African Region continues to bear a large and disproportionate share of the global burden of this disease. In 2021, 95% of malaria cases and 96% of malaria deaths were recorded in this Region. Children under 5 accounted for 80% of all deaths due to the disease. In Senegal, the year 2021 saw a rise in malaria cases in the country, from 445,313 cases in 2020 to 536,850 in 2021 [3] . Malaria is one of the leading causes of death in children. The severe form is fatal if not treated early and appropriately. Several factors are thought to be associated with these deaths, including bacterial superinfection, severe and poorly tolerated anemia, hypoglycemia, respiratory distress and coma. Since 2015, the national malaria control program has recommended the use of artesunate as a first-line molecule in the etiological treatment of malaria, in line with WHO recommendations. This study will assess the impact of artesunate treatment on mortality, as well as changes in factors associated with death, in comparison with previous studies, in a context of improving the quality of emergency care. In this context that we conducted this descriptive and analytical study over a 6-month period from July 1 to December 31, 2022, in the emergency department of the Centre Hospitalier National d’Enfants Albert Royer, the main pediatric referral facility for severe forms of malaria in Dakar.

The aim of our study was to investigate the clinical and biological factors associated with mortality during severe childhood malaria in the emergency department of the Albert Royer National Children’s Hospital in Dakar, Senegal. Multivariate

RDT = Rapid Diagnostic Test, CRP = C-Reactive Protein, MCV = Mean Corpuscular Volume, CSF = cerebro-spinal fluid, UCBE = Urine Cyto-Bacteriological Examination.

analysis showed 3 clinical factors strongly associated with death in children hospitalized for severe malaria in the emergency department. These were disorders of consciousness, respiratory distress and renal failure. There was no significant association between death and the other clinical and biological items, according to the results of the statistical analysis. Impaired consciousness (coma) was statistically associated with the risk of death in our study (OR = 2.28 95% CI [1.31 - 3.96]. This result is in line with the literature [4] . Coma results from sequestration of red blood cells in the cerebral circulation, with disruption of cerebral perfusion and oxygen supply [5] . This leads to neuronal damage, with cerebral edema and intracranial hypertension, which can result in brain stem compression and cardiorespiratory arrest. These phenomena justify the higher risk of death in children with these disorders of consciousness, as opposed to those without. In addition the risk of hypoglycemia, which make worse brain damage, and the risk of intracranial hypertension. Hypoglycemia in malaria results from 2 mechanisms: the use of quinine as an antimalarial drug induces insulin production and hypoglycemia, and the disruption of hepatic gluconeogenesis due to rheological disorders [6] . Other factors contributing to hypoglycemia include digestive disorders (diarrhea, vomiting, refusal to eat, anorexia) and dehydration. Respiratory distress (RD) was also identified as a factor associated with death during severe malaria in children hospitalized in emergency departments (OR = 2.18 95% CI [0.40 - 11.91]). In severe malaria, it generally results from an acute respiratory distress syndrome of lesional origin, leading to acute pulmonary edema with hypoxemia [7] . This picture of hypoxemic lesional edema often requires respiratory assistance, which is not always the case in our resource-constrained countries. Other factors increase the risk of death: refractory hypoxemia, in particular severe malarial anemia, and septic shock. Acute renal failure was the 3rd factor identified as associated with a risk of death among our patients in our study (OR = 4.5 95% CI [1.53 - 13.10]). Renal damage in malaria results from the phenomena of globular sequestration, but is favored by the occurrence of dehydration and hemodynamic disorders. The risk associated with renal damage is the occurrence of ionic complications such as hyponatremia and hyperkalemia. These ionic disorders can be complicated by intracranial hypertension and cardiac arrhythmia, leading to cardiorespiratory arrest and death [8] . These results have been reported by authors in Africa, notably Senegal, with young age (P = 0.025), coma (P = 0.007), respiratory distress (P = 0.04) or hypoglycemia (P = 0.001) as risk factors for death [7] . Other clinical and laboratory signs of severity were not associated with a higher risk of death, according to our results. It would be desirable to carry out a multicenter study over a longer period with a more representative sample in order to be able to draw conclusions. In our study, the most frequent signs of neurological severity were prostration, observed in 32 of our patients (41.03%). This result was similar to those reported by Senegalese authors [9] [10] . Other neurological signs were less frequent, including seizures and coma (10.26% and 7.69% respectively). Jaundice was found in 33.33% of our patients. Clinical anemia was noted in 57 patients (73.08%). The frequency of anemia in malaria can be explained by the importance of hemolysis due to high parasite density, the association with martial deficiency in Africa due to malnutrition. Circulatory collapse was found in 6.41% of our patients, a higher rate than the 1.8% reported by other authors [9] . Macroscopic hemoglobinuria was found in 2.56% of cases, a lower rate than that reported by some authors, 10.18% and 6.9% [9] [11] . Biologically, microscopy was the reference test for the biological diagnosis of malaria according to WHO recommendations. It was positive in all patients, with a mean parasite density of 7462 parasites per micro-liter of blood (720 - 110,000 parasites/microliter). These results are in line with the literature [12] . In addition to microscopy, RDT was also routinely performed on all children admitted with fever. It is less sensitive than microscopy, but is recommended as first-line treatment by Senegal’s national malaria control program [3] for all children admitted to emergency departments with fever. It was positive in 87.18% of children, while 10 children had a negative RDT (12.82%). These negative RDT results against a positive microscopy may suggest the occurrence of an HRP2 gene mutation, which is not yet the case in Senegal, although it has been described in some African countries [13] [14] . However, prevalence studies are needed to confirm or refute this hypothesis. Ionic disorders were observed in some children: hyponatremia (39.74%), hypernatremia (1.28%), hypokalemia (8.97%), hyperkalemia (2.56%). These ionic disorders are frequently described as complications of severe malaria [8] [15] . In terms of etiological treatment, almost all patients were treated with injectable artesunate in 93.59% of cases (73/78), while 6.41% (5 patients) were treated with artemether. As recommended by the WHO, the first-line treatment of severe malaria is based on the use of injectable artesunate [12] [15] . The majority (92.30%) had a favorable outcome. The mortality rate was 7.69% for patients admitted late, or with comorbidity or bacterial superinfection. However, our study has some limits. These include the small sample size (78 patients), the monocentric nature of the study, and the fact that the study period only covered the second half of the year (6 months). A prospective multi-center study with a more representative sample would enable us to confirm these findings through statistical analysis.

Severe malaria is still fatal in our health facilities. Some factors have been identified as being associated with this mortality. A prospective multicenter study with a more representative sample will confirm these results.

The authors declare no conflicts of interest regarding the publication of this paper.

Thiongane, A., Ba, A., Ly, F., Ndongo, A.A., Boiro, D., Kéita, Y., Basse, I., Niang, B., Ly, I.D., Dieng, Y.J., Cissé, D.F., Seck, N., Thiam, L., Faye, P.M., Fall, A.L., Sylla, A. and Ndiaye, O. (2023) Descriptive and Analytical Study of Factors Associated with Mortality in Severe Malaria among Children in Dakar Emergency Departments from July to December 2022. Open Journal of Pediatrics, 13, 866-878. https://doi.org/10.4236/ojped.2023.136095