In the present investigation, soybean seeds from ten most used varieties with the greatest affinity by producers in the Canindeyú and Alto Paraná region were chosen.

Among them M5947 IPRO, M5705IPRO and M64I61 IPRO characterized by their high productive potential, early stability, aggressive root system and good health.

NS6483 and NS6906IPRO; both with high productive potential at early sowing dates, in addition to having optimal branching power, stable and rustic in lower fertility environments, resistant to diseases such as stem canker; while the NA5909 RR, characterized by its precocity, with high productivity and the possibility of staggering sowing, high stability in different environments, in addition to being resistant to diseases such as frog’s eye spot, macrophhomina and phytophthora.

DM62R63; good stability and excellent yield potential, strong behavior against overturning since it has good vegetative bearing and adapts to different environments.

SOJAPAR R49, highly productive in areas where there is not good fertility, resistant to stem canker and Asian rust.

The 59I60 RSF IPRO - BMX DELTA IPRO characterized by its high performance, branching, precocity and health.

HO Pirapó (64HO114 IPRO), which stands out for having the highest performance potential within the product portfolio on the market. It has an adaptation for all regions of the country and is more compact, with short internodes; That is, it has no problems with tipping over.

The pathogen Colletotrichum truncatum was isolated from soybean seeds with signs of anthracnose and they were inoculated in potato dextrose agar (PDA) culture medium for multiplication, developing in approximately 5 to 7 days. Isolation was carried out by streaking the surface of Petri dishes, incubated at a temperature of 28˚C. The spores of the fungus were taken from the culture medium and mixed and homogenized in 15 ml of sterile distilled water, and then filtered through cheesecloth to eliminate the agar or remains of mycelium and brought to a final volume of 100 ml. Several counts were carried out in a Neubauer chamber by taking a drop with a Pasteur pipette, releasing an average of 3 × 10⁴ spores per ml. The spore suspension obtained from the pure culture was adjusted to a concentration of 1 × 10⁴ spores ml⁻¹, the concentration used for the inoculation of the experiments; using the formula C₁ × V₁ = C₂ × V₂, considering C₁ as the initial concentration (known in the count) V₁ = initial volume (established arbitrarily when preparing the inoculum) C₂ = desired final concentration V₂ = final volume (unknown).

Soybean seeds intended for inoculation and control were surface sterilized with 10% chlorine for 3 min. They were subsequently rinsed three times with sterile distilled water and allowed to dry for 1 h under a laminar flow chamber.

The seeds intended for infection were immersed for 1 h in a spore suspension (1:2 w/v) of Colletotrichum truncatum, followed by drying for 3 hours.

The experiment was constituted by a factorial arrangement A × B (Table 1) where A indicates with and without artificial inoculation of Colletotrichum truncatum of soybeans and B ten varieties of soybeans arranged in a completely randomized design, with twenty treatments and four repetitions, totaling 80 units experimental, distributed completely randomly. Each experimental unit consisted of 100 seeds, which were sown on germitest paper, measuring 28 × 38, left in Germination ovens with 80% humidity at 25˚C - 26˚C, suitable for good

development of a seedling, in accordance with the established by ISTA (International Seed Testing Association) rules.

The physiological quality for both the controls and the inoculated ones was recorded 6 to 8 days after being placed in the oven on germitest paper. Therefore, the inoculated seeds were incubated for 6 - 8 days after artificial inoculation by C. truncatum following the above procedure and considered as infected seeds for this study.

To carry out the germination test, the methodology and criteria indicated by ISTA (2016) were used.

The substrates (germitest paper) containing the seeds were kept inside closed boxes, wrapped in plastic bags or placed directly on trays in the germination chamber, taking care that the relative humidity of the site could be maintained very close to saturation. During the permanence of the substrates, the necessary care was taken to ensure that they will not dry out and that they contain sufficient moisture for the entire analysis period.

Once the stipulated time (8 days) for the analysis arrived, the paper rolls were removed from the germinator for reading and evaluation, where the seedlings were classified into; normal seedlings (PN), abnormal seedlings (PA), hard seeds (SD), dead seeds (SM), fresh seeds (SF). The results were recorded in the Internal Germination Bulletin, expressed as a percentage, considering that the sum of the percentages of PN and PA and SD, SF and SM must give 100%.

For this evaluation, the Accelerated Aging (EA) methodology indicated by [9] was followed for a period of 48 hours. This laboratory technique is composed of 2 well-defined stages where the first step was to subject the seed to a period of stress at high temperature and relative humidity and a second period of germination under optimal conditions. The test lasts 7 days for EA (accelerated aging).

For the stress period, 11.0 × 11.0 × 3.5 cm gerbox boxes were used that were clean and disinfected with alcohol. Inside each box, 40 ml ± 1.0 ml of water was placed, then the grids were placed inside and the soybeans on top of them, where they were distributed in such a way that they did not overlap. To finish the process, the boxes were covered and transferred to the Biochemical Oxygen Demand (BOD) at a temperature of 41˚C ± 0.3˚C for 48 h ± 15 minutes.

During the period that the boxes were in the BOD, care was taken to ensure that the seeds did not come into contact with water, and that there was a space of approximately 2.5 cm between the plates to guarantee uniformity in temperature distribution.

After 48 hours, sowing was carried out on germitest paper, where the methodology is the same as that used in the PG evaluation, leaving the germitest rolls inside the germinator for 5 days until the evaluation. During the time that the substrates were in the germination chamber, the temperature control of the germination chambers was carried out, recorded for the control bulletin. The evaluation of the seedlings, the calculations and the issuance of results were carried out following the criteria established for the Glycine max germination test.

This variable was evaluated using the Tetrazolium salt test (TZ), which basically consists of hydrating the seed to generate its physiological activity (respiration). The function of the tetrazolium salt will allow living cells to stain a reddish hue, which will indicate potential germination capacity. So, dead cells will not be stained.

In order to expose the embryo to the direct action of tetrazolium, the seed was subjected to a longitudinal incision, with subsequent staining of these, which were immersed in a solution of 2,3,5-triphenyltetrazolium chloride (tetrazolium) concentration. 0.75%, for which 75 ml of stock solution is diluted in 925 ml of water.

The samples were packed in previously moistened germination paper and pre-conditioned for 16 hours at 25˚C in the germinator, so that they can absorb water. After the immersion time had elapsed, the seeds were separated from the solution using a small strainer and washed previously with distilled water to remove excess dye [10] .

For the evaluation, a cut was made in the soybean seed longitudinally crossing the embryonic axis to expose the central cylinder, removing the integument and observing the external and internal surface of the cotyledons to determine possible damage to the seeds that may be from mechanical damage, moisture damage and bed bug damage. These damages are represented by the type and level of staining which are classified into eight levels. From level five onwards, the seed will be considered unviable; in the event that there is an absence of red color, this will indicate lower viability or death of the embryo.

The samples of inoculated and non-inoculated seeds were ground to obtain fine flour, and then sent to the Inpasa S.A laboratory, to carry out the oil extraction using the distillation method with petroleum ether. This methodology consisted of extracting three grams of each sample, and transferring them to a 10 × 10 cm qualitative paper for distillation in the distiller with petroleum ether, a process that lasts 6 hours until obtaining 100% pure oil. Once the oil was extracted from the sample, the analysis was carried out by gas chromatography for the evaluation of esters, where the percentage of saturated and unsaturated fatty acids and the influence of the pathogen C. truncatum on the chemical composition of the seed were observed.

The data obtained were statistically analyzed with the analysis of variance (ANAVA) for which the use of the SAS statistical software was used [11] . The comparison of means was carried out using the Tukey test at 5% error.

The results of the percentage of germination of soybean seeds are seen in Table 2, which shows that there was a highly significant difference according to the analysis of variance between the treatments in relation to this variable.

In (factor A) it was observed that the seeds without inoculation presented a higher percentage of vigor of 55% and there was a significant difference in relation to the seeds that were inoculated, with a difference of 14.4% at less vigor.

In relation to inoculation (factor B), a significant difference has also been presented. The SOJAPAR R49, NS 6483 and NS6906 IPRO varieties obtained better performance compared to the other varieties.

The results of the percentage of viability of the soybean seed varieties are seen in Table 3. It is observed that there was no significant statistical difference according to the analysis of variance between the treatments in relation to this variable; however, in factor B it was observed that some varieties obtained a low percentage of viability.

Values of the means followed by the same letters do not differ statistically from each other according to the 5% Tukey test.

Values of the means followed by the same letters do not differ statistically from each other according to the 5% Tukey test.

The results of the evaluation of esters in the soybean seed are shown in Table 4. It is observed that there was a significant difference for both Factor A and Factor B according to the analysis of variance between the treatments in relation to this variable.