Food waste was collected from the Kramer Dining Center, Kansas State University, for 6 consecutive weeks resulting in 30 daily samples. Food waste was initially identified and split into two categories or streams: vegetable preparation (VP) from the kitchen and main (MS) from the serving line. The VP consisted of any food scraps leftover from preparing meals or fruit and vegetables that may have gone bad such as onion peels, pepper stems, whole apples, grapes, or celery stalks. This also consisted of seasonal foods such as melon rinds and pineapple tops. The MS consisted of finished foods that had been out on the buffet line for students and either could not be reheated, repurposed, or donated to a local food pantry. This also consisted of food that could have been burned during the cooking process or the recipe was not followed correctly. Some examples of MS food are combinations of pizza, pasta, rice, scrambled eggs, salad bar fixings, and various desserts. During the collection period, waste was kept separate at the dining center according to streams VP and MS. Each day, food waste was brought back to the lab where it was weighed and ground to a homogenous particle size using either a food processor for VP or a meat grinder (LEM #8, 0.5 HP) for MS. The VP was immediately dried in a 13˚C oven to a constant weight and ground (Bliss Hammermill, Ponca City, OK, 6 mm screen) for a more uniform particle size. Subsamples were taken from MS, dried in a 13˚C oven, and ground (Hamilton Beach Coffee Grinder). Daily VP and MS samples were analyzed for dry matter (DM, AOAC 930.15), acid detergent insoluble crude protein (ADICP, ANKOM Technology Method 14 and LecoTruMac N Macro), neutral detergent insoluble crude protein (NDICP, ANKOM Technology Method 15 and LecoTruMac N Macro), neutral detergent fiber (NDF, ANKOM Technology Method 15), lignin (ANKOM Technology Method 9), and crude fat (EE, AOCS AM 5-04) at Dairy One Forage Testing Laboratory (Ithaca, NY). Ash (AOAC 942.05), crude protein (CP, AOAC 990.03) using a nitrogen analyzer (FP928, LECO Corporation, St. Joseph, MI), and gross energy (GE, Parr 6200 Calorimeter, Parr Instrument Company, Moline, IL) were analyzed at Kansas State University. Nutrient concentrations from VP and MS were used to calculate a weighted value for a hypothetical mixed food waste stream (MX) as follows:

MX ( Znutrient ) = ( WeightofMS Totaldailyweight ) ∗ % ofMSZnutrient + ( WeightofVP Totaldailyweight ) ∗ % ofVPZnutrient

Nitrogen-corrected neutral detergent fiber (NDFn), total digestible nutrients (TDN), digestible energy (DE), and metabolizable energy (ME) were calculated using the following equations:

N D F n = N D F − N D I C P [12]

T D N = 0.98 ∗ ( 100 − N D F n − C P − A s h − E E ) + e ( − 0.012 ∗ A D I C P ) ∗ C P + 2.25 ∗ ( E E − 1 ) + 0.75 * ( N D F n − L i g n i n ) ∗ ( 1 − ( L i g n i n N D F ) 0.667 ) − 7 [12]

D E = 4168 − 9.1 ∗ A s h + 1.9 ∗ C P + 3.9 ∗ E E − 3.6 ∗ N D F [13]

M E = 4194 − ( 9.2 ∗ A s h ) + ( 1.0 ∗ C P ) + ( 4.1 ∗ E E ) − ( 3.5 ∗ N D F ) [13]

In order to compare food waste streams to common ruminant and monogastric feedstuffs, nutrient composition of 29 commonly used feedstuffs were sourced from the Nutrient Requirements of Beef Cattle: Eighth Revised Edition [14] and the Nutrient Requirements of Swine: Eleventh Revised Edition [13] . Nutrient concentrations (CP, ash, EE, lignin, NDF, TDN (cattle), and ME (swine)) for each feedstuff were weighted to each food waste stream’s respective nutrient resulting in the absolute relative difference between each nutrient in the feedstuff and food waste stream then summed to give an aggregated relative difference for each food waste stream by feedstuff. The feedstuff with the aggregated absolute relative difference closest to zero was considered the most comparable to the food waste stream. The following equation was used to determine the absolute relative difference of each nutrient then summed together to create an aggregate relative difference for each feedstuff.

Relativedifference ( z ) = | Foodwaste ( z ) − Feedstuff ( z ) | Foodwaste ( z )

All data were analyzed using R statistical software (version 4.2.2). Descriptive statistics were computed using the describe function of the psych package. The levene Test function of the car package was used to test for homogeneity of variance and a pairwise comparison of variances was performed using the pairwise.var.test in the RVAidMemoire package. The gls function of the nlme package was used to compare nutrient concentrations of food waste streams. Due to unequal variances, the varIdent function was used to generate variance coefficients which were used to describe the within-group heteroscedasticity structure in the gls model. The joint_tests and emmeans function of the emmeans package were used to generate an ANOVA table from the gls model object and calculate the least square means using a Tukey adjustment and the Kenward-Roger approximation. Means and variances were considered significant at P ≤ 0.05.

Food waste was separated into three streams for two initial reasons. First, the Food and Drug Administration passed the Modernization Act of 1997 which prohibits the use of mammalian protein products being fed back to ruminant animals to reduce the risk of bovine spongiform encephalopathy (BSE) infection of additional animals [15] . There are exemptions, however, which include meat products that have been cooked for the use of human consumption [16] . This is because the meat products that were intended for human consumption have been inspected, cooked, and further processed and are considered to pose little risk. So, while food waste is considered exempt from this law, there is no way to guarantee that the food did not come into contact with other meat products or trimmings that were not cooked. Additionally, producers understandably might not want to take that risk for fear of infection and public opinion even if it could be guaranteed that it was all cooked thoroughly. And secondly, separating the streams improves processing efficiency. For example, the VP stream was greater in NDF content than the MS source (P < 0.05) and therefore waste could not be ground in a meat grinder as was done with MS. The two streams required different equipment that could handle the two extremes of fiber content.

Table 1 illustrates each of the three waste streams broken down by their average nutrient content, standard deviation, and minimum and maximum value on a dry matter basis. Nutrient values reported for this study were slightly increased from previously conducted research [7] . However, a similar study collecting cafeteria food waste at the State University of New Jersey found very similar nutrient composition to the reported values in Table 1 showing that location or palate variability could be a potential factor when considering its usefulness in a livestock diet [17] . Moisture content and EE had wide ranges of reported concentrations that could cause concern. All three waste streams contained high moisture that ranged considerably between the minimum and maximum. For example, main stream fluctuated from 44.4% to 93.9% moisture while VP only spanned from 81.0% to 96.0% moisture. Salmonella and other bacteria rapidly multiply in feed that has a water activity level of 40% or greater [18] . This immediately causes concern for rapid spoilage and the need for prompt processing methods. There are multiple processes that could be used to remove excess water in a timely fashion to avoid spoilage such as dry extrusion, spray drying, and dehydration. However, more research would be needed to determine the most

¹Neutral detergent insoluble crude protein (NDICP); ²Neutral detergent fiber (NDF); ³Nitrogen free neutral detergent fiber (NDFn); ⁴Acid detergent insoluble crude protein (ADICP); ⁵Total digestible nutrients (TDN); ⁶Gross energy (GE); ⁷Digestible energy (DE); ⁸Metabolizable energy (ME).

effective method while maintaining the nutrient value. Another nutrient worth noting is the amount of EE in the streams, specifically the MS, which ranged 5.9% - 68.8%. While EE is a valuable and expensive component to formulating diets, if it is consistently high it may limit the potential use of food waste. Increasing supplemental fat in feedlot finishing diets to 8% has been shown to decrease ruminal and total tract digestion of organic matter, ADF, and fat which in turn can reduce feed intake due to increased amounts of ruminal solid retention [19] . Thus, finishing diets are commonly formulated at or below 6% total fat. Another factor to consider when potentially feeding high levels of fat is the type of fat. Pigs fed beef tallow have firmer bellies than pigs fed soybean oil, and unsaturated fats, which also, in turn, impacts the marketability of the product [20] . Most dishes are prepared with vegetable oils that have relatively high concentrations of polyunsaturated fat, and thus, is a concern when using food waste in swine diets. There are certain fat capturing processing methods that are used by biodiesel industries which mainly source soybeans for their oil. These methods could potentially be implemented with food waste to capture value for other markets; however, an economic analysis would be needed to determine if a fat expelling step would be beneficial or detrimental to the overall value of the food waste as an energy source. Another limitation of the ability to feed food waste is the high fiber content VP, specifically NDF and NDFn. Lignin, however, was not different (P > 0.05) among the three waste streams. High fiber feedstuffs and diets for swine are known to lower energy density and digestibility, decrease protein digestibility, and increase the passage rate resulting in less time for digestion and absorption of nutrients [21] [22] [23] [24] .

Table 2 compares the nutrient concentrations of the food waste streams. Mainstream and MX had greater (P < 0.05) concentrations of DM, EE, NDICP, and CP than VP. Meanwhile, VP had a greater (P < 0.05) concentration of ash than MS and MX, and greater (P < 0.05) amounts of NDF and NDFn than MX which also had a greater (P < 0.05) amount than MS. In both equations by Noblet and Perez (1993) that were used to determine digestible and metabolizable energy, ash and NDF, which were greater (P < 0.05) in VP, were subtracted from the total energy value while CP and EE, which were greater in MS and MX, were added to the total energy value. Not surprisingly, MS had greater (P < 0.05) energy (GE, DE, and ME) and TDN concentrations than MX, and MX had greater (P < 0.05) energy and TDN than VP. There was no difference between the three streams for ADICP (P = 0.641), but MS had less (P < 0.05) lignin than VP while MX was intermediate.

Table 3 compares the variability in nutrient content of food waste streams. The MS had greater (P < 0.05) standard deviation compared to VP, and MX and MX being increased compared to VP for both DM and EE. The VP had the lowest standard deviation compared to MS and MX for NDICP, TDN, and GE (P < 0.05). However, the standard deviation for NDF, NDFn, CP, ash, lignin, ADICP, DE, and ME were not different (P > 0.05).

To further test compatibility of feedstuffs that are commonly used to the food waste streams, 29 feedstuffs were identified, and their aggregated absolute relative difference was calculated and ranked (Table 4). It is important to note that each feedstuff evaluation had a different amount of submitted samples thus creating slight variability between sources. This led to the decision to keep feedstuffs and sources separate by species to keep relevancy. The lowest aggregated absolute relative difference was considered the most similar to the respective food waste stream while the highest difference was considered the least similar to the respective food waste stream. The vegetable preparation stream was most similar to citrus pulp for both cattle and swine (aggregated relative difference of 1.40 and 2.04, respectively). More specifically, citrus pulp was most common to VP because of its ash (0.21 [cattle] and 0.10 [swine] relative difference), NDF (0.14 [cattle] and 0.11 [swine] relative difference), lignin (0.09 relative difference for cattle), TDN (0.07 relative difference for cattle), and ME (0.04 relative difference for swine) concentrations. CP (0.55 and 0.52 relative difference for cattle and swine, respectively) and EE (0.35 and 0.27 relative difference for cattle and swine, respectively) were the least compatible nutrients.

The most compatible cattle feedstuff for MS and MX was bakery by-product (aggregated relative differences of 2.54 and 1.84, respectively). Of the nutrients,

^abcMeans without a common superscript within a row differ at P-value < 0.05; ¹Neutral detergent insoluble crude protein (NDICP); ²Neutral detergent fiber (NDF); ³Nitrogen free neutral detergent fiber (NDFn); ⁴Acid detergent insoluble crude protein (ADICP); ⁵Total digestible nutrients (TDN); ⁶Gross energy (GE); ⁷Digestible energy (DE); ⁸Metabolizable energy (ME).

^abcStandard deviations without a common superscript within a row differ at P-value < 0.05; ¹Main food waste stream; ²Vegetable preparation food waste stream; ³Mixed food waste stream; ⁴Neutral detergent insoluble crude protein (NDICP); ⁵Neutral detergent fiber (NDF); ⁶Nitrogen free neutral detergent fiber (NDFn); ⁷Acid detergent insoluble crude protein (ADICP); ⁸Total digestible nutrients (TDN); ⁹Gross energy (GE); ¹⁰Digestible energy (DE); ¹¹Metabolizable energy (ME).

Common feedstuffs values for cattle were sourced from the Nutrient Requirements of Beef Cattle: Eighth Revised Edition (National Academies of Science, Engineering, and Medicine, 2016). Common feedstuffs values for swine were sourced from the Nutrient Requirements of Swine: Eleventh Revised Edition (NRC, 2012). ^1,2,3,4,5The absolute relative difference between CP, ash, EE, NDF, lignin, TDN (cattle), and ME (swine) of the feedstuff and food waste stream were summed to give an aggregated relative difference for each food waste stream. Feedstuffs that are most similar to the respective food waste stream have the lowest value, feedstuffs that are the least similar have the highest value. Highest and lowest values per stream are in bold.

lignin (0.03 [MS] and 0.07 [MX] relative difference) and TDN (0.18 [MS] and 0.11 [MX] relative difference) were the two most compatible nutrients followed by ash (0.38 [MS] and 0.46 [MX] relative difference) and CP (0.39 [MS] and 0.36 [MX] relative difference). Ether extract of bakery by-product was only 0.60 relative difference for MS and 0.50 relative difference for MX. However, NDF relative difference was much greater in the bakery by-product than the MS and MX (0.95 and 0.35, respectively). On the other hand, the least compatible cattle feedstuff when compared to MS, VP, and MX waste streams was cottonseed hulls (aggregated relative difference of 21.39, 10.93, and 17.56, respectively). The two main factors contributing to the difference with cottonseed hulls were the NDF and lignin content. The NDF concentration of cottonseed hulls is 11, 3, and 7 times that of the MS, VP, and MX streams, respectively. Additionally, lignin was 9, 7, and 9 times greater than the MS, VP, and MX streams, respectively. Meanwhile, nutrients such as CP, TDN, EE, and ash were all less than 50% of their respective waste stream nutrient concentration with three exceptions: the ash concentration being 55% of the MS ash content, the TDN concentration being 56% of the VP TDN content, and the EE concentration being 72% of the VP EE content.

Other cattle feedstuffs that came comparatively close (less than +2 points from the lowest value mentioned above) for all three food waste streams were grain sorghum, hominy feed, steam-flaked corn, and wheat. Other feedstuffs that were comparable to VP but not MS or MX were corn gluten feed, corn silage, oats, and wheat bran and middlings, again, all less than +2 points from the value of citrus pulp. These feedstuffs had similar relative differences in TDN and EE concentrations to VP, with the exception of EE in oats.

The most compatible swine feedstuff for MS and MX streams was wheat (aggregated relative difference of 3.25 and 2.66, respectively). Of the nutrients, CP (0.25 [MS] and 0.20 [MX] relative difference) and ME (0.28 [MS] and 0.21 [MX] relative difference) were the two most compatible nutrients, followed by lignin (0.47 [MS] and 0.49 [MX] relative difference) and NDF (0.68 [MS] and 0.16 [MX] relative difference) which exceeded the MS and MX NDF content. The two least compatible nutrients of wheat were ash (0.66 [MS] and 0.70 [MX] relative difference) and EE (0.92 [MS] and 0.90 [MX] relative difference). On the other hand, the least compatible swine feedstuff was whole cottonseed for all three waste streams (aggregated relative difference of 12.50, 10.02, and 9.82 for MS, VP, and MX, respectively). The NDF content of whole cottonseed is 7, 2, and 5 times that of the NDF content of MS, VP, and MX waste streams, respectively. Additionally, lignin content of wheat is 5, 4, and 5 times that of lignin in the MS, VP, and MX waste streams, respectively. Crude protein of wheat exceeded all three waste streams (0.19 [MS], 0.69 [VP], and 0.25 [MX] relative difference). Ash (0.34 [MS], 0.54 [VP], and 0.42 [MX] relative difference), EE (0.30 [MS], 3.73 [VP], and 0.12 [MX] relative difference), and ME (0.30 [MS], 0.11 [VP], and 0.23 [MX] relative difference) content were all less than their respective food waste streams nutrient content with the exception of EE which was 4 times that of VP EE content and ME of VP (3207 vs. 2895 kcal/kg).

Additional swine feedstuffs worth noting that are similar, but not the closest, to all three waste streams, are grain sorghum, ground corn, and oat groats, all less than +1.5 from citrus pulp and wheat, as mentioned earlier. Soybean meal was a close comparison to the MS and MX streams, but not VP. Meanwhile, wheat, while not the most comparable to VP, was less than +0.5 from citrus pulp. Other feedstuffs that came very close in comparison to VP were wheat bran, wheat middlings, oats, and corn gluten feed, notably for the similarities in CP, EE, and ME.