In the face of the current domestic family planning policy of China’s comprehensive opening, the number of pregnant people has increased significantly. Infertility is a relatively common disease of the reproductive system, which tends to occur in couples of childbearing age. In recent years, the incidence of this disease has gradually increased due to the influence of lifestyle, living environment and eating habits [1]. Infertility is related to many factors, such as ovulation disorder, fallopian tube obstruction and low sperm quality, which seriously affect the quality of life and family happiness. At the same time, it will also bring different degrees of negative impact on the population growth of China. Therefore, early diagnosis and timely treatment has become the focus of research [2]. In this study, 60 infertile couples who came to our hospital for diagnosis and treatment from June 2021 to June 2022 were selected for analysis, and the application value of the combined detection method of sperm quality, sex hormone and ovulation was discussed. The details are as follows.

Research shows that [4,5], couples of childbearing age who do not take contraceptive measures have an average 25% chance of pregnancy in each menstrual cycle, 50% should be able to conceive within 3 months after marriage, 72% should be able to conceive within 6 months after marriage, 80% - 85% should be able to conceive within 12 months after marriage, and infertility may affect about 10% of couples of childbearing age. Therefore, if you have regular sex for more than one year after marriage without taking any contraceptive measures, and your spouse is unable to conceive or can conceive but fails to conceive and deliver, you should consider the possibility of infertility, which is collectively referred to as infertility in medicine, including male infertility and female infertility. Affected by living conditions, work pressure, personal bad living habits and other factors, the incidence of infertility is gradually on the rise. With the proposal of national policies, eugenic birth and eugenic rearing, it is necessary to strengthen the examination of infertility in couples of childbearing age and determine the causes of infertility [6,7].

Male infertility is mostly related to sperm quality decline, testicular damage, varicocele, reproductive tract infection, etc. Sperm quality includes quantity, activity and morphology [8]. According to the new WHO semen testing manual, the sperm concentration should be at least 15 million/ml, the semen volume should be ≥1.5 ml, and the forward motile sperm should be ≥32%. In terms of sperm morphology, the healthy sperm should be ≥4%, and the malformation rate should be <96%. In daily life, smoking, drug taking, alcoholism, drug abuse, extramarital sexual behavior and other bad habits will affect the secretion of male hormones, harm the gonads and reproductive tract, induce erectile dysfunction or loss of libido, sperm malformation, and eventually cause male infertility [9,10]. Female infertility is mainly caused by the lesions of reproductive system related tissues or diseases of uterus and ovary. In addition, ovulation dysfunction and endocrine diseases related to sex hormone abnormalities are also the main problems of clinical female infertility [11]. Studies have shown that [12] ovulation dysfunction occurs in 15 percent of all pregnant couples, and up to 40 percent of female infertility can lead to significant menstrual disturbances (infrequent menstruation or amenorrhea), and may also reduce symptoms. The most common causes include polycystic ovary complex (PCOS), obesity, weight gain or loss, strenuous exercise, thyroid dysfunction, hyperprolactinemia, etc. Therefore, it is necessary to combine clinical experience and theoretical knowledge to select appropriate examination methods to diagnose infertility, help improve the patient’s pregnancy function and meet their fertility requirements [13]. According to the research results, the data show that the low sperm activity, low concentration, low survival rate and high abnormal sperm index of infertile men will affect the fertility quality. In the sex hormone examination indicators, it can be found that there are abnormalities in the sex hormone indicators in the observation group. FSH and LH are related to the control of reproductive activities; LH has the role of ovulation promotion; Together with FSH, it acts on the corpus luteum and secretes progesterone; The abnormally high level of FSH indicates premature ovarian failure; The low level of LH indicates that there is dysfunction in the pituitary or hypothalamus, which leads to infertility [14,15]. Serum E2 is an important indicator for monitoring menstrual abnormalities. By observing serum sex hormone indicators, we can understand the functional status of hypothalamus and ovary, predict ovulation time and evaluate ovarian function, and also help clinicians understand the changes and progress of female infertility [16,17]. Anti-mullerian hormone (AMH) is a dimeric glyconeptide, also known as Mullerian duct inhibitor (MIS), which is mainly produced by granulosa cells and has the highest expression in secondary follicles, presinus follicles and small sinus follicles. The level of AMH is relatively stable within and during the menstrual cycle and is an excellent indicator for assessing ovarian reserve. In view of ovulation, vaginal Doppler ultrasound technology is used to understand the size of the ovary and the number of follicles with the help of high-frequency probes. By monitoring the development of follicles, we can observe whether they are factors affecting female infertility, and also observe whether there are abnormalities in the structure of the uterus and ovary. If the ovary is small and the number of ovulations is small, it indicates that abnormal ovulation will lead to decreased fertility. In addition, vaginal ultrasound can also monitor ovarian reflux; It plays an important role in predicting ovulation and evaluating ovarian function [18,19]. The detection methods of three groups have relatively accurate diagnosis results, but there are also subjectivity, misdiagnosis, missed diagnosis and other problems. The research results show that, compared with the single indicators of three groups, the detection rate of joint testing for 60 cases of couples is higher (P < 0.05), indicating that in order to better improve the diagnosis of infertility and help patients develop more reasonable and effective treatment methods, multiple joint testing can provide more diagnostic basis for patients’ follow-up treatment, and help to meet the pregnancy needs of patients [20,21].

To sum up, for the diagnosis of infertility, the combined detection method of sperm quality, sex hormone and ovulation can effectively identify the problems of both couples, significantly improve the detection rate of patients’ causes, which can be further promoted and applied in clinical practice.

The main business of the Reproductive Health Research Center is to carry out work around human assisted reproductive technology. Most of the selected research objects are infertile people, with a narrow regional scope. The research results obtained do not represent the conventional level of normal and healthy semen of men who have given birth, but also have certain limitations. It is necessary to expand the scope of research, or select men who have naturally had a first child as the research object, which is more representative.

During the process of this topic research, we got much help from many departments and individuals, and other personnel not involved in this project research. All of them offered a great support and help in this research. Now here, all of members in this research show our deepest appreciation to them, and wish them good health and everything goes well.

The project funded by the Administration of Traditional Chinese Medicine of Guangxi Zhuang Autonomous Region in 2021 (No.: GXZYZ20210050).

The authors declare no conflicts of interest regarding the publication of this paper.