Background: This paper describes and displays the spinal radiological anatomy and associated pathology in a
Macaca fascicularis and compares it to the spinal anatomy of humans. Animal models are commonly used in research. As compared to
Macaca mulatta, the anatomy of
M. fascicularis is less well described in the literature. Materials and methods: The authors anatomically reconstructed and reviewed the defleshed spine of a single adult
M. fascicularis visually, radiographically, and with high resolution CT. Results: 7 cervical, 12 thoracic, 6 lumbar, 3 sacral, and 16 caudal vertebrae were identified. Similarities in the spine to humans were seen as well as differences such as the beaked anterior arch of C1, the anterior pointed lower lumbar vertebrae, the upward curved transverse processes, and presence of three sacral segments. Degenerative changes were seen at multiple locations similar to humans though most pronounced at T3-4. Conclusions: This paper addresses the normal spinal anatomy and degenerative changes in an adult
M. fascicularis and compares it to humans.
Animal models advance the understanding of human pathology, physiology, behavior, and development. These models have an important role in advancing medical knowledge in diverse fields such as veterinary and human imaging, organ and tissue transplant, vaccine development and medical devices [1]. The choice of animal is based on similarity of physiology, neuroanatomy, reproduction, development, cognition and social complexity [2].
Of the vertebrate animal models used in research, 90% are mice, rats and or other rodents [2]. Non-human primates (NHP) are less commonly used, but have an important role. This is due to the genetic, immunologic, physiologic, and endocrine similarities between this species and human beings. Macaca mulatta and Macaca fascicularis are commonly used in scientific research. M. fascicularis, or cynomolgus macaque [3] is the most commonly used species for testing the safety of drugs [1].
M. fascicularis and humans are genetically similar. Genetic mapping studies have demonstrated a 92.83% sequence similarity when comparing the M. fascicularis and human genomes [4]. Gene mapping has found 11,446 one-to-one orthologous genes between M. fascicularis and humans [5]. Genes corresponding to Alzheimer’s disease, epilepsy, schizophrenia, HIV infection and multiple tumors were found to be orthologous [5]. The close genetic relationship between M. fascicularis and human beings is indicative of the common ancestral history between the two species. M. fascicularis have been extensively used in research in pharmacology, development, physiology, oncology, skeletal pathology, neurology, ophthalmology, plastic surgery, as well as, gene expression [4] - [10].
While the anatomy of the M. mulatta has been well described in the literature [11] [12] [13] [14], the anatomy of M. fascicularis has received less attention and published information regarding M. fascicularis particularly on radiography and CT is lacking. Given the important role that M. fascicularis play in biomedical research, this paper aims to fill this gap by describing and displaying the spinal anatomy and associated pathology in a M. fascicularis individual and compare it to human spinal anatomy and relevant pathology.
2. Materials and Methods
A cadaveric defleshed and disinfected skeleton of an adult M. fascicularis, born in captivity, was obtained from the New England Regional Primate Research Center. The cadaveric skeleton was derived from an animal that was euthanized as a component of a previous scientific protocol. At Beaumont Hospital, Royal Oak, the authors anatomically reconstructed the various portions of the skeleton using principles, similar to the human skeleton. In concert with a radiology physicist, the reconstructed skeleton was then evaluated in a number of ways including visually (Figure 1), radiographically (Figure 2), and finally utilizing high resolution multidetector Computed Tomography (CT) (Figures 3-15). CT, imaging, as a model, enhances the understanding of living tissues in humans and
animals as well as cadaveric tissues. CT scans were taken using an ultra high resolution (UHR) protocol on a Siemens Definition Flash scanner (Erlangen, Germany). The UHR images were acquired using a 16 × 0.3 mm beam, 120 kVp, 225 effective mAs, and a pitch = 1. Images were reconstructed with a thickness of 2 mm using the U75u algorithm and a 140 mm field of view (300 mm scan field of view). Digital radiographs were taken on a GE Definium 8000 (General Electric Healthcare, Chicago, IL). Images were acquired with a 50 kVp and 2 mAs setting, using the small focal spot. Images were acquired using the “Hand” protocol for basic bone enhancement. Since there was no soft tissue, the technique was lowered. CT images were reviewed on a PACS (picture archiving and communication system) station. As the specimen lacked soft tissue (such as intervertebral discs) to closely align the vertebrae, additional reconstructions of select vertebrae was performed in order to mimic the alignment of vertebrae in the spine. This was accomplished using Balsa wood and tape (chosen to reduce CT artifacts but supportive of the vertebrae). These vertebrae were also scanned on the CT scanner, and axial, coronal, sagittal, and select 3D reconstructions were obtained to better facilitate assessment.
3. Results3.1. Overview and Cervical Spine
The M. fascicularis skeleton depicts many similarities to the human skeleton. The spine of the M. fascicularis in particular shares many commonalities with the human spine. The M. fascicularis spine has seven cervical vertebrae (Figures 6-9). The vertebrae include spinous processes, transverse processes and bodies similar to human vertebrae. Additionally, the vertebrae also depict vertebral arches created by laminas and pedicles as found in humans. The C1 vertebra lacks a vertebral body, similar to the human C1, while the other cervical vertebrae have small bodies. Grooves for the vertebral arteries were seen over the superior surface of the C1 vertebra posteriorly (Figure 6). The anterior arch of C1 in our specimen demonstrated in contrast to humans a protuberance (Figure 6, Figure 9) that has a beaklike appearance (triangular on the transverse images) with a flattened inferior surface on the sagittal images. The C2 vertebra demonstrates a structure similar to the odontoid process found in humans with a similar articulation with C1. Some arthritic changes were seen near the median C1-2 articulation (Figure 8). The transverse processes of the C2 and C3 vertebrae were well defined, longer than in humans, but similar to humans angled caudally. Foramen transversarium for the vertebral arteries were found in the transverse processes of the M. fascicularis C1-C6 vertebrae as is seen in the human body. Of slight difference from human cervical vertebrae, the C6 vertebra lacks a bifid spinous process. The C7 vertebra, in our specimen, demonstrated the longest spinous process, but only slightly greater than C6, in contrast to the typically prominent spinous process of C7 in humans known as vertebra prominens.
3.2. Thoracic Spine
Our specimen depicts twelve rib bearing thoracic vertebrae. Of note, 12 ribs were seen on the right side and 10 were seen on the left side; this may be due to anatomical asymmetry of the number of ribs and/or loss during processing. The upper thoracic vertebrae demonstrate somewhat small and horizontal transverse processes. The lower thoracic vertebrae, for example T11 (Figure 10), demonstrate somewhat slender and inferiorly directed transverse processes. The vertebral body is also more rounded with a circular spinal canal. The first thoracic vertebra has a long spinous process. Additionally, the spinous processes of the thoracic vertebrae are long and slope postero-inferiorly. These properties of the M. fascicularis thoracic vertebrae are similar to characteristics found in the human thoracic vertebrae. The last two thoracic vertebrae were the largest.
3.3. Lumbar Spine
This specimen depicted six non rib bearing lumbar vertebrae (Figures 11-13). These vertebrae depict larger bodies as compared with the thoracic and cervical vertebrae. The upper lumbar vertebrae show some features similar to the lower thoracic vertebrae though with small transversely oriented transverse processes. Progressively, the transverse processes in the lumbar vertebrae become longer and curve superiorly, particularly prominent at L4 through L6 in our specimen. This appearance, to us, mimics the horns of a “Viking Helmet” (Figure 13). Similar to the lumbar vertebrae found in humans, the M. fascicularis lumbar vertebrae have a large spinal canal, and short spinous processes. Additionally while the upper lumbar vertebrae have a relatively reniform vertebral body, the lower lumbar vertebrae have a more beak like appearance anteriorly. This is distinct and different from those in humans.
3.4. Sacrum and Caudal Segments
The sacrum depicts structures analogous to the sacral promontory, and median sacral crest found in the human sacrum. As opposed to the human sacrum, which is composed of five fused sacral segments and 4 sacral foramina, the M. fascicularis specimen depicts three fused sacral segments and two sacral foramina (Figure 5). We identified a total of 16 caudal vertebrae and a few tiny ossific fragments, which we believe make up the tail of the M. fascicularis. Caudal vertebrae in primates are described as being made of three parts, the proximal, transition, and distal. The first 4 segments constitute the proximal caudal vertebrae. Of these, the first three demonstrate a neural arch, with downsloping and well developed transverse processes and superior and inferior facet joints. The transition vertebra which is part of the proximal demonstrates an arch, but has facet joints only superiorly. The next two are the transitional vertebra that lack a neural arch but have articulations. The second of these is the longest vertebra. The remaining vertebrae progressively decrease in size and are the distal caudal segments of which we were able to identify 10. These distal caudal segments on cross sectional imaging, depicted a groove at one end and 4 ridge like protuberances, probably representing the two small transverse processes and the bifid spinous process.
3.5. Pathological Findings
The vertebrae of this specimen also demonstrates degenerative or senescent changes similar to those seen in humans. Some of the vertebral bodies depict loss of height, endplate sclerosis, and osteophytosis. In our sample, degenerative changes were best seen at the C1-2, C7, T2, T3, T4, T8, and L4 levels with most pronounced findings at T3-T4 (Figure 14, Figure 15). Sclerosis was seen along the adjoining endplates of T4 and T3. A focus of subchondral cyst formation along the inferior endplate of T3 was also seen along with the osteophytes. These changes are reminiscent of osteoarthritic changes noted in humans with spinal disk degeneration. The disks could not be evaluated as we reviewed only the osseous elements.
4. Discussion
The M. fascicularis specimen described had seven cervical vertebrae, twelve rib-bearing thoracic vertebrae, and six lumbar vertebrae. Human beings typically have 5 lumbar vertebrae, although this is subject to some anatomical variation. In humans, numbering of vertebrae is essential before surgery to perform procedural treatment at the correct level (for example in spinal fusion with instrumentation, facet joint injections, etc.) [15]. Often, whole spine radiographs or in the case of MRI, cervico-thoracic localizers are used to accurately number the spine from C2 caudally. The first 7 vertebrae are labeled as cervical, as the cervical spine demonstrates morphological stasis [16] [17]. Typically, radiologists number the next rib bearing vertebrae as thoracic; this is typically 12, but there is some variability, with some as many as 13, based on the presence of the extra singular or paired rib(s). Of note, humans also occasionally demonstrate a pair of cervical ribs but these are not usually confused given their location articulating with the C7 vertebrae.
Following the thoracic vertebrae, the next non-rib bearing vertebrae, by convention, are referred to as lumbar vertebrae. In this M. fascicularis specimen, we identified twelve thoracic and six lumbar vertebrae. In related research (personal communication by author SN), we have noted that some M. fascicularis subjects have 7 lumbar vertebrae.
While human beings typically have five lumbar vertebrae, numerical variants, as well as, lumbosacral transitional vertebrae (LSTV) are common. The prevalence of LSTV is approximately 30% of the general population; this is often associated with numerical variants in the number of lumbar vertebrae [18]. An understanding of the evolution and presence of LTSV and numerical variants in humans through research in NHP would be illuminating; these anatomical variations, in human medicine, have been contributors to errors in procedural and surgical treatments [16]. Transitional vertebrae have also been associated with early degeneration of intervertebral discs above the transitional vertebrae. This phenomenon is associated with altered biomechanics [19] and low back pain (Bertolotti syndrome). This is a disorder that affects young people and is refractory to medications and as well interventional pain treatment [20] [21].
Additionally, numerical variants and LSTV have been found to be associated with dermatomal variation and an alteration in nerve function [4].
Osteoarthritic changes commonly seen in the human spine on imaging were also observed in our specimen. As has been described in the literature [22], disc pathology is different according to the site in spine. It is well described in humans in the cervical spine with endplate sclerosis, and osteophyte formation [22] [23]. Thoracic disease is less common than cervical or lumbar disease [22]. Rhesus macaques show more prominent age related kyphosis in the lower thoracic spine with osteophyte formation especially along the anterior aspect of the vertebrae, in contrast to humans [24] [25]. The M. fascicularis is interesting in that it sometimes assumes an erect position similar to humans [22]. In our specimen, the most pronounced degenerative changes were at T3-4. This is also the level that is believed to have the smallest disc space [22]. This osteoarthritic process lends NHP models to research, addressing interventions for degenerative disease. Current treatment in humans with degenerative spine conditions diseases consist primarily of combination of lifestyle changes, physical interventions, pharmacologic pain relief, as well as procedural and surgical treatment; the long term efficacy of these treatments is subject to significant controversy. While these interventions provide some relief to patients, they do not completely address the underlying pathophysiology.
The M. fascicularis is a particularly appropriate candidate for studies in degenerative bone diseases not only due to similar anatomy, as noted in this study, but also due to similarity in bone structure itself. Among various animals including rabbits, dog, and non human primates studied by microcomputed tomography and radiology, M. fascicularis bone structure, density and microanatomy were most similar to that of humans, making them the most desirable species to conduct preclinical research [26]. This is also helpful in orthopedic research in the spine and knee. Importantly, following open knee surgeries and knee joint cartilage sampling, M. fascicularis have been demonstrated to be appropriate models for age-related knee osteoarthritis [6]. Anatomical findings that made M. fascicularis an appropriate model for knee osteoarthritis may also make it a fit for studies into degenerative diseases overall.
The tail of the M. fascicularis also presents further opportunities for discovery. This species has a particularly long tail with twenty-six vertebrae with a variation of three to six vertebrae [27]. Consistent with descriptions in the literature [27], the first 4 had a neural arch and are referred to as proximal, including the transition vertebra, followed by the two transitional and long vertebrae and finally the distal caudal vertebrae. It is possible that in processing a few caudal fragments were lost as we identified a total of 16 caudal segments in our specimen. Alternatively, there may be biological variability accounting for the difference between our specimen and literature. The tail is supplied by 4 long nerves that have motor neuron pools in the spinal cord. The length of the tail can yield information regarding peripheral nerve pathology and possible treatments [28] [29]. Tail length variations amongst macaque species, have an evolutionary basis; it is considered an adaptive mechanism for thermoregulation and balance during locomotion [27]. These longer tail lengths lend themselves to the need for a more developed peripheral nervous system, with the existence of longer neurons for control of the tail. In turn, these longer neurons of the long tailed M. fascicularis as compared to the short tailed M. mulatta can better facilitate nerve conduction and similar studies in research.
5. Limitations
This study is based on a single skeleton. There is biological heterogeneity related to the anatomic structures in animals as with humans. However, to our knowledge, this is the only CT assessment of the skeleton of the M. fascicularis, which we hope will stimulate better understanding of these animals.
6. Conclusion
This paper addressed the skeletal structure of the spine, of a M. fascicularis specimen with an effort to describe both normal anatomy, and degenerative changes. These findings can be extrapolated to other parts of the skeletal system to facilitate further orthopedic, skeletal and neurology research. The similarities in the skeletal structure and genetic identities of M. fascicularis and humans suggests that using this NHP species as a model can provide invaluable information regarding human pathologies and possible treatments. This paper has the limitation of analyzing a single individual M. fascicularis. Without other specimens, it is difficult to draw conclusions regarding the skeletal structures of M. fascicularis as a species. Despite these limitations, this description provides insight into the skeletal structure of the spine of M. fascicularis and suggests future directions of research.
Location of Study
Anatomical reconstruction of skeleton bones and scanning was done at Beaumont Hospital, Royal Oak (described also in materials and methods).
Conflicts of Interest
The authors declare no conflicts of interest regarding the publication of this paper.
Cite this paper
Krishnan, A., Kaleka, G., Emerson, S., Sovak, G., Simmons, H., Brunner, K., Schalk, D., Sledge, J., Hoggatt, A. and Nesathurai, S. (2022) Anatomical and Radiological Description of the Macaca fascicularis Spine in Comparison with the Human Spine. Open Journal of Veterinary Medicine, 12, 171-186. https://doi.org/10.4236/ojvm.2022.1212014
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