<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article  PUBLIC "-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" "http://dtd.nlm.nih.gov/publishing/3.0/journalpublishing3.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" dtd-version="3.0" xml:lang="en" article-type="research article"><front><journal-meta><journal-id journal-id-type="publisher-id">AJPS</journal-id><journal-title-group><journal-title>American Journal of Plant Sciences</journal-title></journal-title-group><issn pub-type="epub">2158-2742</issn><publisher><publisher-name>Scientific Research Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.4236/ajps.2022.137069</article-id><article-id pub-id-type="publisher-id">AJPS-118710</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biomedical&amp;Life Sciences</subject></subj-group></article-categories><title-group><article-title>
 
 
  Effect of Maturity Indices on Growth and Quality of High Value Vegetables
 
</article-title></title-group><contrib-group><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>A.</surname><given-names>K. M. Quamruzzaman</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Ferdouse</surname><given-names>Islam</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Limu</surname><given-names>Akter</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" xlink:type="simple"><name name-style="western"><surname>Sharmilla</surname><given-names>Rani Mallick</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib></contrib-group><aff id="aff2"><addr-line>Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh</addr-line></aff><aff id="aff1"><addr-line>Olericulture Division, Horticulture Research Center, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh</addr-line></aff><pub-date pub-type="epub"><day>11</day><month>07</month><year>2022</year></pub-date><volume>13</volume><issue>07</issue><fpage>1042</fpage><lpage>1062</lpage><history><date date-type="received"><day>12,</day>	<month>May</month>	<year>2022</year></date><date date-type="rev-recd"><day>22,</day>	<month>July</month>	<year>2022</year>	</date><date date-type="accepted"><day>25,</day>	<month>July</month>	<year>2022</year></date></history><permissions><copyright-statement>&#169; Copyright  2014 by authors and Scientific Research Publishing Inc. </copyright-statement><copyright-year>2014</copyright-year><license><license-p>This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/</license-p></license></permissions><abstract><p>
 
 
  The study took place at Bangladesh Agricultural Research Institute’s Olericulture Division’s research farm from March 2021 to February 2022 (BARI). In a protected net house, we investigated the impact of five different types of vege
  tables on various maturation stages, including tomato, broccoli, sweet peppe
  r, cucumber, and netted melon. Vegetables cultivated under p
  rotected conditions in a transparent poly
  -
  film net
   
  house can improve quality, maturit
  y, fruit size, and yield. When fruits and vegetables are picked before they are fully mature, they may stay green for longer, but they may not ripen to a satisfactory color and flavor, resulting in a loss of consumer confidence. Furthermore, because fruit continues to grow until 
  the 
  harvest, immature fruit will be smaller than mature fruit, reducing harvest yield. We tried to determine the right
   maturation stages in order to avoid product loss during our investigation. The tomato was found to be an appropriate size (6.5 cm length and 6.2 cm diameter), weight (84 g), TSS (4.5 percent), pH (4.3), 
  “
  turning red”, and
   
  “tasty” at the week 5 stage, while the broccoli was found to be an appropriate size (12.0 cm length and 13.0 cm diameter), weight (360 g), and “green” color at the week 5 stage. At the week 6 stage, 
  the 
  nettled melon was found to be of appropriate size (15.2 cm length and 14.5 cm diameter), weight (800 g), TSS (10.8 percent), pH (6.3), “net fully developed” on the fruit skin and
   
  “much tasty,” while cucumber was found to be of appropriate size (8.8
   
  -
   
  10.8 cm length and 2.2
   
  -
   
  2.9 cm diameter), weight (61
   
  -
   
  88 g), TSS (3.8
   
  -
   
  4.1 percent), pH (6.3), 
  “
  less powdery
  ”.
   As a result, establishing the optimal maturity of our research will benefit both consumers and growers.
 
</p></abstract><kwd-group><kwd>Maturity Indices</kwd><kwd> Growth</kwd><kwd> Quality</kwd><kwd> High Value Vegetables</kwd><kwd> TSS</kwd><kwd> pH</kwd><kwd> Different Harvest Stages</kwd></kwd-group></article-meta></front><body><sec id="s1"><title>1. Introduction</title><p>In order to acquire high-quality produce and productivity during the off-season, vegetables must be grown in protected environments such as greenhouses or poly net homes [<xref ref-type="bibr" rid="scirp.118710-ref1">1</xref>]. Poly net buildings have the potential to be used economically to grow high-value temperature-sensitive veggies all year. Because of their better productivity and economic feasibility, poly net houses are commonly used for the cultivation of high-value vegetables. Due to the manipulation of the spectra of radiation reaching the crops, protected poly net houses allow for better utilization of sunlight, promoting physiological responses in plant and fruit development, such as leaf area index, chlorophyll and carotenoid content, tissue structure, fruit ripening, physiological disorders, nutritional quality, and so on [<xref ref-type="bibr" rid="scirp.118710-ref2">2</xref>]. Protected, naturally ventilated high-tunnel cultures are ideal for growing high-value crops in the off-season [<xref ref-type="bibr" rid="scirp.118710-ref3">3</xref>]. Plastic tunnels, both high and low, have long been a useful tool for crop diversification and season extension, allowing growers to create a microclimate more conducive to warm-season crops [<xref ref-type="bibr" rid="scirp.118710-ref4">4</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref5">5</xref>]. Because plants are planted more consistently, big gaps between plants and rows are minimized, and light interception is optimized, a higher fruit yield/unit area can be attained in a high-tunnel protected culture than in a field [<xref ref-type="bibr" rid="scirp.118710-ref6">6</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref7">7</xref>]. Poly homes have a yield potential of 1.5 times that of open fields [<xref ref-type="bibr" rid="scirp.118710-ref8">8</xref>]. When compared to the previous year, total fruit yields (t/ha) increased by 113 to 131 percent.</p><p>Identifying the correct stage of maturity and harvesting at the appropriate time are critical pre-harvest criteria for getting the most out of the covered net houses. Maturity indices are useful for determining when a certain item should be harvested in order to provide some marketing flexibility and ensure that the consumer receives appropriate eating quality. Maturity is the stage of growth that leads to a consumer’s attainment of a specific goal. Physiological maturity: When a plant or a plant part has all of the necessary characteristics to be used (Selvakumar [<xref ref-type="bibr" rid="scirp.118710-ref9">9</xref>]). In a physiological sense, maturity refers to a plant portion or the entire plant reaching the last stage of biological activity. Vegetable quality can be retained for a longer amount of time if gathered at the right time [<xref ref-type="bibr" rid="scirp.118710-ref10">10</xref>]. Harvesting of tomatoes, for example, is done at different times depending on the function of the fruit. Vegetables are typically harvested at various stages of maturity for long-distance sales, local markets, fresh consumption, or consumer preferences. For long-distance marketing, the tomatoes are collected at a ripe green to turning stage. Pink to light-red tomatoes are picked for the local market.</p><p>Determination of maturity indices: Maturity can be judged by various means, like: 1) Computational methods [a) Calendar date, b) Days after anthesis/pollination]; 2) Physical methods [a) Increase in size, b) Color development, c) Softening of tissues, d) Seediness, e) Development net-like structure, f) Yellowing and drying of foliage or top, g) Flowering and bolting]; 3) Chemical methods [a) Increase in sugar content, b) Increase in fiber content, c) Increase in sugar: acid ratio]; 4) Physiological methods [a) Respiration rate, b) Ethylene evolution rate]. Thus, farmers ought to schedule the harvesting at optimum maturity periods to ensure quality and obtain a good market price, followed by the correct handling and packing of fruit [<xref ref-type="bibr" rid="scirp.118710-ref11">11</xref>]. Despite this, most farmers, especially those operating on a small scale, face challenges, among which are the choice of the right varieties, ineffective transport to distant markets, and high perishability of products, exacerbated by harvesting at improper maturity stages due to farmers’ limited knowledge of maturity indicators [<xref ref-type="bibr" rid="scirp.118710-ref12">12</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref13">13</xref>]. Non-temperature controlled plastic tunnel net houses can be used due to their low cost and simplicity as they use natural ventilation to reduce temperature [<xref ref-type="bibr" rid="scirp.118710-ref14">14</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref15">15</xref>]. Natural ventilation, however, may not sufficiently reduce the heat load [<xref ref-type="bibr" rid="scirp.118710-ref15">15</xref>].</p><p>Determining the best maturity stage is a challenging practice due to the high variability found in crops. Based on technologies available nowadays, the more relevant maturity indices are coloration, sugars and acids content (evaluated as the ratio between both, TSS:acid ratio), and juice content (as a percentage). Maturity indices also depend on the destination markets and the growing regions, therefore, there are no universal or absolute values, and the different producer countries may apply variable maturity standards. The distance to the market and consequently, transportation time is one of the more important parameters to consider when making harvest decisions [<xref ref-type="bibr" rid="scirp.118710-ref16">16</xref>].</p><p>There is a scarcity of data on the maturation indicators of many high-value vegetables for qualitative qualities in protected habitats. Because the selected crops, such as tomato, broccoli, sweet pepper, cucumber, and netted melon, are high-value vegetables in Bangladesh, they were chosen for poly net homes to ensure high-quality production. The goal of this study is to calculate the maturity indices for quality vegetable production in Bangladesh. With this in mind, the current study was designed to determine the maturity indices for quality tomato, broccoli, sweet pepper, cucumber, and netted melon production in Bangladesh.</p></sec><sec id="s2"><title>2. Materials and Methods</title><sec id="s2_1"><title>2.1. Experimental Site</title><p>The evaluation site was the research farm of the Olericulture Division, Bangladesh Agricultural Research Institute (BARI) from Mar 2021 to Feb 2022. The field was at 23.9920˚N Latitudes and 90.4125˚E Longitudes having an elevation of 8.2 m from sea level under the agro-ecological zone (AEZ) 28. The farm was situated in the sub-tropical climatic zone and characterized by scanty rainfall during the experimental period. The soil of the experimental field was sandy clay loam in texture having a pH range of around 6.0.</p></sec><sec id="s2_2"><title>2.2. Air Temperatures and Relative Humidity of the Experimental Area</title><p>Under protected conditions, temperatures can be monitored and managed, and better plant growth could be expected. The protected net house condition influenced the air temperature and RH. Data for the temperatures and RH were measured at 12 pm daily during the experimental period. The average minimum and maximum temperatures varied between 25.8˚C to 36.8˚C, while relative humidity varied between 62.1% to 81.5% in day time (<xref ref-type="fig" rid="fig1">Figure 1</xref>).</p></sec><sec id="s2_3"><title>2.3. Plant Materials</title><p>Five types of vegetables, viz., tomato, broccoli, sweet pepper, cucumber, and netted melon, were cultivated in UV stabilized transparent polyethylene film with a 60-mesh insect net house. Different intervals of harvest were followed for different vegetables for this study. Fruit and quality related traits were visually observed and categorized. Fruits were selected randomly from tagged plants and picked to measure fruit length, diameter, average weight, TSS, and pH. Fruits were harvested on different days after flower opening.</p></sec><sec id="s2_4"><title>2.4. Flower Tagging and Fruit Harvest</title><p>Flowers were tagged at anthesis to determine the stage of fruit development, and 10 randomly selected fruits from 10 plants were harvested at specific day intervals according to the vegetable to determine their growth and maturity. At each harvesting date, 10 fruits were used to determine physicochemical properties like fruit length, diameter, weight, TSS, and pH.</p></sec><sec id="s2_5"><title>2.5. Measurement of Fruit Weight, Length, and Diameter</title><p>Fruit weight was determined using a digital electronic balance (Mettler PJ400, Switzerland) after transporting the harvested fruit in a closed plastic bag to the laboratory. Fruit length and diameter were measured with a digital vernire caliper.</p></sec><sec id="s2_6"><title>2.6. Measurements of Total Soluble Solids (TSS) and pH</title><p>The total soluble solids and pH of the juice were determined by using a hand-held Kruss refractometer (Model HR 900, SN 1200793, brix range 0% - 90% at 20˚C, Germany) and a glass electrode pH meter (Delta 320, Mettler, Shanghai), respectively.</p></sec><sec id="s2_7"><title>2.7. Evaluation of Sensory Attributes</title><p>Sensory evaluation, based on general visual appeal, colour, crispiness, flavor, and taste, was conducted. The scores were: 7 = like extremely; 6 = like very much; 5 = like moderately; 4 = neither like nor dislike; 3 = dislike moderately; 2 = dislike very much and 1 = dislike extremely. Fruit scored above 4 was considered acceptable. Sensory evaluation was performed by a panel of judges consisting of 5 scientific personnel, including both male and female members. Different preferences, as indicated by scores, were evaluated by statistical methods.</p></sec><sec id="s2_8"><title>2.8. Statistical Analysis</title><p>Using the R 3.6.3 statistical software, the data was subjected to analysis of variance (ANOVA).The results showing significant differences were then subjected to mean separation using Tukey’s Studentized Range (HSD) Test at P ≤ 0.05.</p></sec></sec><sec id="s3"><title>3. Results and Discussion</title><p>Harvesting at an optimal stage gives the productive and commercial sectors greater flexibility for their management [<xref ref-type="bibr" rid="scirp.118710-ref17">17</xref>]. Climacteric fruits develop their full characteristic flavor, taste, and color during storage if picked during an optimum period. These fruits, harvested at an early stage of maturity, are susceptible to shriveling and mechanical damage and develop poor flavor and taste, despite having a long storage life [<xref ref-type="bibr" rid="scirp.118710-ref18">18</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref19">19</xref>]. Harvesting at an advanced stage of maturity produces fruits that have good taste and flavor but have a short storage life and are not suitable for transporting long distances [<xref ref-type="bibr" rid="scirp.118710-ref20">20</xref>]. The climacteric is a stage of fruit ripening associated with increased ethylene production and a rise in cellular respiration. Alexander and Grierson [<xref ref-type="bibr" rid="scirp.118710-ref21">21</xref>] state that broccoli and netted melon are climacteric fruits, whereas eggplant, cucumber, okra, and sweet pepper are not (they ripen without ethylene and respiration bursts).</p><sec id="s3_1"><title>3.1. Tomato</title><p>Fruits measurement of tomato fruits</p><p>Fruit size is a quality parameter used to determine maturity stage at different times of harvest. Generally, at the early stage of growth development, fruits remain small and immature, and at the later stage, the fruits become bigger to attain maturity. An increase in fruit length, fruit diameter, and fruit weight was observed in parallel in this study.</p><p>The fruit development curve for tomatoes was determined (<xref ref-type="fig" rid="fig2">Figure 2</xref>). Following fruit setting, there was a slow increase in fruit diameter and length in the first week. After that, it increased in week 2. Further increases in fruit length and diameter occurred in week 3, reaching a maximum towards the end of week 5 of fruit maturation. The lowest fruit size was at the week 1 stage (1.7 cm and 1.5 cm), followed by week 2, week 3, week 4, week 5 and week 6 (2.4 and 2.1 cm; 3.8 and 3.3 cm; 5.2 and 4.9 cm; 6.5 and 6.2 cm), while the highest fruit size was 6.6 and 6.3 cm at a later stage of harvest (week 6) (<xref ref-type="fig" rid="fig2">Figure 2</xref>). But this stage is not</p><p>suitable for fresh consumption; the fresh consumption stage is week 5 (6.5 and 6.2 cm). The changes in fruit diameter and length are expected as tomatoes mature. Zhang and McCarthy [<xref ref-type="bibr" rid="scirp.118710-ref22">22</xref>] stated that tomato ripening is usually associated with a number of variations in the cellular structure and internal structure of the fruit. In addition, the most significant visual changes in the morphological characteristics of the fruit during maturation occur in the size, shape, length, and volume of the fruit as it advances in age [<xref ref-type="bibr" rid="scirp.118710-ref20">20</xref>]. This therefore means that fruit length and diameter are important indices for determining tomato maturity. The fruit weight was gradually increased with the increase of maturity. At the earliest stage (week 1), the individual fruit weight was only 15 g, while the maximum weight (91 g) was at the week 6 stage (<xref ref-type="fig" rid="fig2">Figure 2</xref>). The weight was increased by 32 g, 45 g, and 65 g at week 2, 3, and 4, respectively. The optimum weight was obtained at the week 5 stage with 84 g.</p><p>According to Wu and Kubota [<xref ref-type="bibr" rid="scirp.118710-ref23">23</xref>], tomato fruit enlarge with time after anthesis during the green stage, reach maximum size at around the end of the green stage, and hardly change in size after the breaker stage through the red stage, as demonstrated in this study. Chester [<xref ref-type="bibr" rid="scirp.118710-ref24">24</xref>] and Lovejoy [<xref ref-type="bibr" rid="scirp.118710-ref25">25</xref>] indicated that several tomato varieties are ready for harvest between 6 - 11 weeks following transplanting. This study indicates that fruit development trends are consistent with earlier findings by Robinson [<xref ref-type="bibr" rid="scirp.118710-ref26">26</xref>], Dadzie and Orchard [<xref ref-type="bibr" rid="scirp.118710-ref20">20</xref>], and Mattheis and Fellman [<xref ref-type="bibr" rid="scirp.118710-ref18">18</xref>]. Suryawanshi [<xref ref-type="bibr" rid="scirp.118710-ref27">27</xref>] indicated that tomatoes should be ready for harvest at least 8.5 weeks following transplanting. The variation in times of harvest may be explained by the differences in soil, weather, and pest and disease incidence across study sites.</p><p>The Total soluble solids index is a quality parameter used to determine the sweetness, ripeness, and marketability of tomatoes. For the commercial market, tomatoes’ TSS should be ≥4% Brix, and below that, tomatoes are not usually suitable. The results (<xref ref-type="fig" rid="fig3">Figure 3</xref>) revealed that overall mean values of TSS in different stages varied from 3.0 - 4.6 percent. At the earlier stages, viz., week 1 to week 3, they were immature stages, while the TSS was 3.0% to 3.9%, with green color, and the taste was astringent. At the week 4 stage, the TSS was 4.1% and the tomato color breaker stage with a slightly astringent taste. Maximum TSS</p><p>was obtained at week 6 (4.6%), but it was over matured at a fully red colored stage. This stage is better for fresh table purposes, but not for long distance purposes. The second highest TSS value was at the week 5 stage (4.5%), while it was the optimum matured turning red stage, and the fresh eaten taste was slightly good. This stage was better for longer distance purposes, not for fresh table purposes. The optimal harvest TSS values at breaker and red agree with Pinho et al. [<xref ref-type="bibr" rid="scirp.118710-ref28">28</xref>] that observed between 4.0 (early harvest) and 6.00 Brix (late harvest) from tomatoes. These results showed a significant difference in TSS content among all stages, which is due to the maturity stage at the time of harvest. The change in TSS with advancement in maturity in this study is attributed to the metabolism of sugar due to increased respiration. The variation in fruit soluble solid content may be due to differences in metabolism and respiration rate between maturity stages. High soluble solids contents are indicators of high sugar content [<xref ref-type="bibr" rid="scirp.118710-ref29">29</xref>]. Fruits are riper, and they contain more flavor volatiles, and these may be useful parameters to predict consumer preference [<xref ref-type="bibr" rid="scirp.118710-ref30">30</xref>]. Harvest at the proper maturity stage is crucial to good eating quality [<xref ref-type="bibr" rid="scirp.118710-ref31">31</xref>].</p><p>The pH of fruit did not change appreciably during maturity stages. However, little difference was observed among the maturity stages. The change in pH was consistent with the passage of time, but there was an increasing trend for all stages gradually. The pH values within treatments varied from 4.2 to 4.7 (<xref ref-type="fig" rid="fig3">Figure 3</xref>). These results coincide with those of Augustin et al. [<xref ref-type="bibr" rid="scirp.118710-ref32">32</xref>] and Beaulieu and Lea [<xref ref-type="bibr" rid="scirp.118710-ref33">33</xref>], who reported a similar pattern of pH values (5.25, 6.51, and 6.79) at different maturity stages. There is a slight variation in pH value of fruit harvested at an immature stage as compared to half matured and fully matured fruit that is attributed to low metabolic rate. There was significant variation among all treatments that showed a decline in pH with advancement of maturity. The TSS and pH content vary as fruit matures [<xref ref-type="bibr" rid="scirp.118710-ref34">34</xref>], making these parameters indispensable maturity indices. Therefore, the maturity indices of tomato fruit are morphological features including length, diameter, age, and color and physico-chemical parameters, notably, TSS and pH.</p><p>The fruit color of tomato was found to be “light green”, “green”, “breaker”, “turning red” and “red” colored at week 1, 2, 3, 4, 5, and 6 stages, respectively, while the organoleptic taste was found to be “astringent” at week 1 to week 4, and “slightly tasty” and “tasty” were found at week 5 and week 6 stages, respectively (<xref ref-type="table" rid="table1">Table 1</xref>).</p></sec><sec id="s3_2"><title>3.2. Broccoli</title><p>Broccoli should be harvested when the head is fully developed, but before the loose head and small yellow flower buds start to open. At harvest, the terminal head should be tight, green, and of an appropriate size. Several smaller side heads (about 1 to 3 inches across) may develop in the axils of the leaves after the central head is removed.</p><p>The broccoli head size is a quality parameter used to determine the maturity stage at different times of harvest. Generally, at the early stage of growth development, the head remains small and immature, and at the later stage, the head becomes bigger to attain maturity. An increase in head length, head diameter, and head weight was observed in parallel. The lowest head size was at the 6 days stage (6.2 cm and 6.8 cm), followed by 8 days, 10 days, 12 days and 14 days (9.2 cm and 9.2 cm; 9.8 cm and 11.0 cm; 12.0 cm and 13.0 cm; 13.0 cm and 15.0 cm), while the highest head size was 13.2 cm and 15.5 cm at the later stage of harvest (16 days) (<xref ref-type="fig" rid="fig4">Figure 4</xref>). But this stage was not suitable for fresh consumption. The fresh consumption stages were 12 days and 14 days (12.0 cm and 13.0 cm; 13.0 and 15.0 cm).</p><p>The head weight and head color were gradually increased with the increase in maturity. At the earliest stage (6 days), the head weight was only 86 g with “dark green” color, while the maximum weight was 420 g at 16 days stage and the color was “green with loose head” (<xref ref-type="fig" rid="fig4">Figure 4</xref> and <xref ref-type="table" rid="table2">Table 2</xref>). The head weight was increased by 173 g, 221 g, 320 g, and 400 g at 8 days, 10 days, 12 days and 14 days</p><table-wrap id="table1" ><label><xref ref-type="table" rid="table1">Table 1</xref></label><caption><title> Fruit color and organoleptic taste of tomato at different harvest stages</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Parameter</th><th align="center" valign="middle"  rowspan="2"  >Qualitative unit</th><th align="center" valign="middle"  colspan="6"  >Harvest stage</th></tr></thead><tr><td align="center" valign="middle" >Week 1</td><td align="center" valign="middle" >Week 2</td><td align="center" valign="middle" >Week 3</td><td align="center" valign="middle" >Week 4</td><td align="center" valign="middle" >Week 5</td><td align="center" valign="middle" >Week 6</td></tr><tr><td align="center" valign="middle" >Fruit color</td><td align="center" valign="middle" >Observed color</td><td align="center" valign="middle" >Light green</td><td align="center" valign="middle" >Green</td><td align="center" valign="middle" >Green</td><td align="center" valign="middle" >Breaker</td><td align="center" valign="middle" >Turning red</td><td align="center" valign="middle" >Red</td></tr><tr><td align="center" valign="middle" >Organoleptic taste</td><td align="center" valign="middle" >Feeling taste</td><td align="center" valign="middle" >Astringent</td><td align="center" valign="middle" >Astringent</td><td align="center" valign="middle" >Astringent</td><td align="center" valign="middle" >Astringent</td><td align="center" valign="middle" >Slightly taste</td><td align="center" valign="middle" >Tasty</td></tr></tbody></table></table-wrap><p>stages, respectively. The optimum head weight was obtained at 12 days with a 360 g “green” colored head, which was found the best quality for edible. No sprouting or looseness was found at this stage. After that, the increase in weight and size occurred in broccoli, but that was not quality due to looseness (<xref ref-type="fig" rid="fig4">Figure 4</xref>). At 14 days and 16 days stage, the head of broccoli was observed to be “green with slight looseness of head” and “green with loose of head”, respectively (<xref ref-type="table" rid="table2">Table 2</xref>).</p><p>Harvesting of broccoli heads at the proper stage is a common problem for growers. If heads are picked when immature, they may stay green longer but may not develop to an acceptable size, which may lead to a loss of consumer confidence. Moreover, since head growth continues until harvest, the size of immature heads will be smaller than more mature heads, resulting in a loss of harvest yield. Over matured broccoli heads quickly lose their attractiveness and shininess, and become slimy in texture [<xref ref-type="bibr" rid="scirp.118710-ref35">35</xref>]. Therefore, determining the optimum maturity will benefit both the consumer and the grower. Normally, broccoli head maturity is assessed on the basis of subjective evaluations of visual colour [<xref ref-type="bibr" rid="scirp.118710-ref36">36</xref>], size, and hand pressing to evaluate firmness or days from anthesis [<xref ref-type="bibr" rid="scirp.118710-ref37">37</xref>].</p></sec><sec id="s3_3"><title>3.3. Netted Melon</title><p>Netted melon (Cucumismelo L.) characteristics include external color, flesh color, firmness, seed cavity tissue, soluble solid content, flavor, aroma, size, and shape [<xref ref-type="bibr" rid="scirp.118710-ref30">30</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref38">38</xref>], unique flavor, shape, and color, melons generally command high prices. These factors determine eating quality and consumer preference [<xref ref-type="bibr" rid="scirp.118710-ref39">39</xref>], and extended seasons could increase grower income.</p><p>The melon fruit remains small and immature at an earlier stage, and at the later stage, the melon fruit becomes bigger to attain maturity. An increase in fruit length, fruit diameter, and fruit weight was observed in parallel in this study. The lowest fruit length and diameter were at the week 1 stage (3.5 cm and 3.2 cm, respectively), followed by week 2, week 3, week 4, and week 5 stages (6.2 and 6.0 cm; 8.4 and 8.0 cm; 10.6 and 10.3 cm; 12.5 and 12.7cm, respectively), while the highest fruit length, diameter were 15.2 cm and 14.5 cm, respectively, at the later stage of harvest (week 6) (<xref ref-type="fig" rid="fig5">Figure 5</xref>). This stage was the right mature stage. The average fruit weight was gradually increased with the increase in maturity. At the earliest stage (week 1). The fruit weight was only 41 g, while the maximum weight was 800 g at the week 6 stage (<xref ref-type="fig" rid="fig5">Figure 5</xref>). The fruit weight was increased by 135 g, 244 g, 397 g, and 577 g at week 2, week 3, week 4, and week 5</p><table-wrap id="table2" ><label><xref ref-type="table" rid="table2">Table 2</xref></label><caption><title> Head color of broccoli at different harvest stages</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Parameter</th><th align="center" valign="middle"  rowspan="2"  >Qualitative unit</th><th align="center" valign="middle"  colspan="6"  >Harvest stage</th></tr></thead><tr><td align="center" valign="middle" >6 days</td><td align="center" valign="middle" >8 days</td><td align="center" valign="middle" >10 days</td><td align="center" valign="middle" >12 days</td><td align="center" valign="middle" >14 days</td><td align="center" valign="middle" >16 days</td></tr><tr><td align="center" valign="middle" >Head color</td><td align="center" valign="middle" >Observed color</td><td align="center" valign="middle" >Dark green</td><td align="center" valign="middle" >Dark green</td><td align="center" valign="middle" >Dark green</td><td align="center" valign="middle" >Green</td><td align="center" valign="middle" >Green with slight loose of head</td><td align="center" valign="middle" >Green with loose of head</td></tr></tbody></table></table-wrap><p>stages, respectively. The optimum fruit weight was obtained at the week 6 stage with 800 g.</p><p>The Total soluble solids index is a quality parameter used to determine the sweetness, ripeness, and marketability of netted melon. For the commercial market, netted melon’s TSS should be ≥10% Brix and below that, netted melon is not usually suitable [<xref ref-type="bibr" rid="scirp.118710-ref40">40</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref41">41</xref>]. The findings (<xref ref-type="fig" rid="fig6">Figure 6</xref>) revealed that the overall mean value of TSS in various stages ranged from 4.5 to 10.8 percent. TSS was 4.5 to 5.3 percent at the earlier stages, namely week 1 to week 4, when the fruit was immature (<xref ref-type="fig" rid="fig5">Figure 5</xref>). Week 5 stage, the TSS started to increase by 9.2% and it was at its maximum at the week 6 stage, with 10.8%. These results showed a significant difference in TSS content among all stages, which is due to the maturity stage at the time of harvest. Beaulieu and Lea [<xref ref-type="bibr" rid="scirp.118710-ref33">33</xref>] also reported an increase in sugar of 5 - 11 percent during ripening in melon. The change in TSS with advancement in maturity in this study is attributed to the metabolism of sugar due to increased respiration. The deviation in the soluble solid content of fruit might be due to variation in metabolism and respiration rate among maturity stages. High soluble solids contents are indicators of high sugar content [<xref ref-type="bibr" rid="scirp.118710-ref29">29</xref>]. Fruits are riper, and they contain more flavor volatiles, and these may be useful parameters to predict consumer preference [<xref ref-type="bibr" rid="scirp.118710-ref30">30</xref>]. Harvesting of melon at the proper maturity stage (when TSS is about 8% - 10%) is crucial to good eating quality [<xref ref-type="bibr" rid="scirp.118710-ref31">31</xref>]. Since the amount of TSS in fruits usually increases as they mature and ripen, the soluble solids content of the fruit can be a useful index of maturity or stage of ripeness for netted melon.</p><p>The pH of fruit did not change appreciably during maturity stages. However, little difference was observed among the maturity stages. The change in pH was consistent with the passage of time, but there was an increasing trend for all stages gradually. The pH values within treatments varied from 5.0 to 6.4 (<xref ref-type="fig" rid="fig6">Figure 6</xref>). These results coincide with those of Augustin et al. [<xref ref-type="bibr" rid="scirp.118710-ref32">32</xref>] and Beaulieu and Lea [<xref ref-type="bibr" rid="scirp.118710-ref33">33</xref>], who reported a similar pattern of pH values (5.25, 6.51, and 6.79) at different maturity stages. A pH of 6.55 occurs at the fruit maturity of melon [<xref ref-type="bibr" rid="scirp.118710-ref42">42</xref>].</p><p>Cantaloupes had a higher pH than honeydew melons. Sweet melons are unique among fleshy fruits with pH values near neutral [<xref ref-type="bibr" rid="scirp.118710-ref43">43</xref>]. There is a slight variation in pH value of fruit harvested at an immature stage as compared to half matured and fully matured fruit that is attributed to low metabolic rate. There was significant variation among all treatments that showed a decline in pH with advancement of maturity. A pH of 6.5 and high soluble solids content are indicators of high sugar content [<xref ref-type="bibr" rid="scirp.118710-ref29">29</xref>]. Fruits are riper, and they contain more flavor volatiles, and these may be useful parameters to predict consumer preference [<xref ref-type="bibr" rid="scirp.118710-ref30">30</xref>]. The TSS and pH content vary as fruit matures [<xref ref-type="bibr" rid="scirp.118710-ref34">34</xref>], making these parameters indispensable maturity indices. Therefore, the maturity indices of fruit are morphological features including length, diameter, age, and color and physico-chemical parameters, notably TSS and pH.</p><p>The net development on the fruit skin of netted melon is a quality parameter used to determine maturity stage at different times of harvest. Generally, at the early stage of growth, the net does not develop well. The net development on fruit skin was gradually increased with the increase of maturity. At the earliest stages (week 1 and week 2), the fruit had a plain surface, while at the mid development stage (week 3), the fruit skin had a “rough surface”. The net development starts at week 4, while the full development of the net was observed at week 5 and week 6 stages (<xref ref-type="table" rid="table3">Table 3</xref>). The melon fruits were tasted at different stages of maturity. The fruit had an “astringent taste” during week 1 to week 3, while it was “slightly tasty”, “tasty”, and “Much tasty” during week 4, week 5, and week 6, respectively. So, from week 5 to week 6 is the appropriate time of maturity to harvest.</p><p>Precise determination of netted melon fruit maturity is difficult at harvest. Fruits are harvested at different degrees of maturity, and a proportion of fruits may be immature. Thus, harvesting of netted melon fruits of different maturities at the same time is a common problem, even though fruits may have the same skin color at harvest [<xref ref-type="bibr" rid="scirp.118710-ref44">44</xref>]. If fruits are picked immature, they may stay green longer but may not develop an acceptable colour and flavour upon ripening [<xref ref-type="bibr" rid="scirp.118710-ref45">45</xref>], which may lead to a loss of consumer confidence. Moreover, since fruit growth</p><table-wrap id="table3" ><label><xref ref-type="table" rid="table3">Table 3</xref></label><caption><title> Net development on fruit skin and organoleptic taste of netted melon at different harvest stages</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Parameter</th><th align="center" valign="middle"  rowspan="2"  >Unit</th><th align="center" valign="middle"  colspan="6"  >Harvest stage</th></tr></thead><tr><td align="center" valign="middle" >Week 1</td><td align="center" valign="middle" >Week 2</td><td align="center" valign="middle" >Week 3</td><td align="center" valign="middle" >Week 4</td><td align="center" valign="middle" >Week 5</td><td align="center" valign="middle" >Week 6</td></tr><tr><td align="center" valign="middle" >Net development on fruit skin</td><td align="center" valign="middle" >Observed color</td><td align="center" valign="middle" >Plain surface</td><td align="center" valign="middle" >Plain surface</td><td align="center" valign="middle" >Rough surface</td><td align="center" valign="middle" >Net developed</td><td align="center" valign="middle" >Net fully developed</td><td align="center" valign="middle" >Net fully developed</td></tr><tr><td align="center" valign="middle" >Organoleptic taste</td><td align="center" valign="middle" >Feeling taste</td><td align="center" valign="middle" >Astringent</td><td align="center" valign="middle" >Astringent</td><td align="center" valign="middle" >Astringent</td><td align="center" valign="middle" >Slightly tasty</td><td align="center" valign="middle" >Tasty</td><td align="center" valign="middle" >Much tasty</td></tr></tbody></table></table-wrap><p>continues until harvest, the size of immature fruit will be smaller than more mature fruit, resulting in a loss of harvest yield. On the other hand, overripe fruits lose their attractiveness and shininess and become slimy in texture within a very short time [<xref ref-type="bibr" rid="scirp.118710-ref35">35</xref>]. Therefore, determining the optimum maturity will benefit both the consumer and the grower. Normally, netted melon fruit maturity is assessed on the basis of subjective evaluations of visual colour [<xref ref-type="bibr" rid="scirp.118710-ref36">36</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref37">37</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref46">46</xref>], size, and hand pressing to evaluate firmness or days from anthesis [<xref ref-type="bibr" rid="scirp.118710-ref37">37</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref47">47</xref>].</p></sec><sec id="s3_4"><title>3.4. Cucumber</title><p>Cucumber for fresh consumption is one of the most popular vegetables worldwide and is a rich source of vitamins, minerals, and antioxidants [<xref ref-type="bibr" rid="scirp.118710-ref48">48</xref>]. It is estimated that one-third of global production is lost or wasted [<xref ref-type="bibr" rid="scirp.118710-ref49">49</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref50">50</xref>]. These losses occur throughout the value chain, from production and collection, transport and storage, to marketing and distribution to the consumer due to postharvest management, while improper maturity is one of the factors among all the steps [<xref ref-type="bibr" rid="scirp.118710-ref51">51</xref>]. To facilitate and improve this process, there are easily applicable indicators that allow the selection of proper maturity stages of cucumbers. Therefore, the objective of this study was to demonstrate the proper maturity stages, which can be effective indicators of the probability of marketability of cucumbers.</p><p>The cucumber remains smaller in size and immature at an earlier stage, and at the later stage, the cucumber becomes bigger to attain maturity. In this study, the lowest fruit length and diameter were obtained at the day 6 stage (5.2 cm and 1.3 cm, respectively), followed by day 8, day 10, day 12, day 14 stages (6.9 cm and 1.8 cm; 8.8 cm and 2.2 cm; 10.8 cm and 2.9 cm; 13.2 cm and 3.8 cm, respectively), while the highest fruit length and diameter were 14.6 cm and 4.3 cm, respectively, at the later stage of harvest (day 16) (<xref ref-type="fig" rid="fig6">Figure 6</xref>). The average fruit weight was gradually increased with the increase in maturity. At the earliest stage (day 6), the fruit weight was only 30 g, while the maximum weight was 148 g at the day 16 stage (<xref ref-type="fig" rid="fig7">Figure 7</xref>). The fruit weight was increased by 45 g, 61 g, 88 g, and 125 g at day 8, day 10, day 12, and day 14 stages, respectively. The maximum fruit weight was obtained at the day 16 stage with 148 g. Cucumber fruits are ready for harvest at the fruit maturity stage of day 10 to day 12 after fruit setting, and it is done at the immature stage of the fruit. In cucumbers, the proper stage of maturity is judged by size and not by the age of the fruit. Cucumbers should be picked when they are 9 - 11 cm long. Another marketable stage is</p><p>when the spines on fruit become soft and fall down. In general, cucumbers may be picked at any stage of fruit growth, provided yellowish has not started. The fruit should be picked at frequent intervals in order to avoid losses due to oversized or overripe fruits. They are typically picked every 2 to 3 days, depending on variety and weather.</p><p>The results (<xref ref-type="fig" rid="fig7">Figure 7</xref>) revealed that overall mean values of TSS in different stages varied from 3.1 - 4.4 percent. At the earlier stages, viz., day 6 to day 8, were immature stages, while the TSS was 3.1 to 3.4 percent (<xref ref-type="fig" rid="fig7">Figure 7</xref>). At a moderate stage of harvest, viz., day 10 to day 12 stage, the TSS was 3.8% to 4.1% and the maximum was at day 14 and day 16 stage with 4.3% to 4.4%. These results showed a significant difference in TSS content among all stages, which is due to the maturity stage at the time of harvest. Beaulieu and Lea [<xref ref-type="bibr" rid="scirp.118710-ref33">33</xref>] also reported an increase in sugar from 5 - 11 percent during ripening in melon. The change in TSS with advancement in maturity in this study is attributed to the metabolism of sugar due to increased respiration. The variation in fruit soluble solid content may be due to differences in metabolism and respiration rate between maturity stages. High soluble solids contents are indicators of high sugar content [<xref ref-type="bibr" rid="scirp.118710-ref29">29</xref>]. Fruits are riper, and they contain more flavor volatiles, and these may be useful parameters to predict consumer preference [<xref ref-type="bibr" rid="scirp.118710-ref30">30</xref>]. The TSS is an important postharvest quality attribute of cucumbers. Since the amount of TSS in fruits usually increases as they mature and ripen, the soluble solid content of the fruit can be a useful index of early maturity or stage of ripeness for cucumbers.</p><p>The powdery surface and spine development on cucumber fruit is a quality parameter used to determine maturity stage at different times of harvest. Generally, at the early stage of growth, the powdery surface and spine development are well developed. The amount of powdery surface and spine development on fruit skin was gradually decreased with the increase of maturity. At the earliest stages (day 6 and day 8), the powdery surface of fruit and spine development were well developed, while at the mid-development stage (day 10 and day 12), the powdery surface of fruit and spine development were less developed. At later development stages (day 14 and day 16), the powdery surface of the fruit was absent and spine development was very low (<xref ref-type="table" rid="table4">Table 4</xref>). The cucumber fruits were tasted at</p><table-wrap id="table4" ><label><xref ref-type="table" rid="table4">Table 4</xref></label><caption><title> Powdery surface of fruit and spine development and organoleptic taste of cucumber at different harvest stages</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Parameter</th><th align="center" valign="middle"  rowspan="2"  >Unit</th><th align="center" valign="middle"  colspan="6"  >Harvest stage</th></tr></thead><tr><td align="center" valign="middle" >Day 6</td><td align="center" valign="middle" >Day 8</td><td align="center" valign="middle" >Day 10</td><td align="center" valign="middle" >Day 12</td><td align="center" valign="middle" >Day 14</td><td align="center" valign="middle" >Day 16</td></tr><tr><td align="center" valign="middle" >Fruit skin color</td><td align="center" valign="middle" >Observed color</td><td align="center" valign="middle" >Powdery surface with spine</td><td align="center" valign="middle" >Powdery surface with spine</td><td align="center" valign="middle" >Less powdery with less spine</td><td align="center" valign="middle" >Less powdery with less spine</td><td align="center" valign="middle" >Very less powdery with less spine</td><td align="center" valign="middle" >No powdery and very less spine</td></tr><tr><td align="center" valign="middle" >Organoleptic taste</td><td align="center" valign="middle" >Feeling taste</td><td align="center" valign="middle" >Less astringent taste</td><td align="center" valign="middle" >Slightly tasty</td><td align="center" valign="middle" >Tasty</td><td align="center" valign="middle" >Tasty</td><td align="center" valign="middle" >Slightly matured</td><td align="center" valign="middle" >Over matured</td></tr></tbody></table></table-wrap><p>different stages of maturity. The fruit was “less astringent in taste” during the day 6 stage, while it was “slightly tasty” at day 8, “tasty” at day 10 and day 12, and slightly matured and over matured at day 14 and day 16 stages, respectively. So, on day 10 and day 12, the appropriate times of maturity to harvest were set.</p><p>Precise determination of cucumber fruit maturity is difficult at harvest. Fruits are harvested at different degrees of maturity, and a proportion of fruits may be immature. Thus, harvesting of cucumber fruits of different maturities at the same time is a common problem, even though fruits may have the same skin colour at harvest [<xref ref-type="bibr" rid="scirp.118710-ref44">44</xref>]. If fruits are picked immature, they may stay green longer but may not develop an acceptable colour and flavour upon ripening [<xref ref-type="bibr" rid="scirp.118710-ref45">45</xref>], which may lead to a loss of consumer confidence. Moreover, since fruit growth continues until harvest, the size of immature fruit will be smaller than more mature fruit, resulting in a loss of harvest yield. On the other hand, overripe fruits lose their attractiveness and shininess and become slimy in texture within a very short time [<xref ref-type="bibr" rid="scirp.118710-ref35">35</xref>]. Therefore, determining the optimum maturity will benefit both the consumer and the grower. Normally, cucumber fruit maturity is assessed on the basis of subjective evaluations of visual colour [<xref ref-type="bibr" rid="scirp.118710-ref36">36</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref37">37</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref46">46</xref>], size, and hand pressing to evaluate firmness or days from anthesis [<xref ref-type="bibr" rid="scirp.118710-ref37">37</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref47">47</xref>].</p></sec><sec id="s3_5"><title>3.5. Sweet Pepper</title><p>Sweet is a non-pungent fruit that is valued for its color, flavor, and nutritional attributes, including ascorbic acid, polyphenolics, and various carotenoids. It comes in a wide variety of colors (ranging from green, yellow, orange, red, and purple), shapes, and sizes, as well as because it has a high content of ascorbic acid, polyphenols, and other antioxidants. Generally, the harvest of sweet peppers is determined by the size, color, and texture of the fruit. Traditionally, the harvest of this fruit is done by reaching physiological maturity when the pericarp becomes thick and the fruit reaches its typical size. However, estimating pepper maturity at the green stage can be difficult, even for fruit with similar physical attributes [<xref ref-type="bibr" rid="scirp.118710-ref44">44</xref>]. The sweet peppers reach their optimum state of maturity for use in the kitchen when they are a solid color. Consumers prefer this fruit at its best stage of maturity, more so than its physical appearance and nutritional content [<xref ref-type="bibr" rid="scirp.118710-ref52">52</xref>]. Dutch researchers specializing in the sensory area, reported that study groups have considered that more ripe sweet peppers are sweeter and have a red pepper aroma, while those in the green stage were rated for the bitterness and aroma of herbs and cucumber [<xref ref-type="bibr" rid="scirp.118710-ref53">53</xref>]. At the mentioned stage, sweet peppers will progress through the normal ripening process to degrade chlorophyll while simultaneously synthesizing carotenoids. For determining an appropriate harvest time, different attributes like surface colour, fruit firmness, soluble solids content, acid content are used as indicators [<xref ref-type="bibr" rid="scirp.118710-ref44">44</xref>], so that products develop acceptable taste and flavour characteristics as well as maintain structural integrity during subsequent storage and/or shelf life [<xref ref-type="bibr" rid="scirp.118710-ref54">54</xref>] [<xref ref-type="bibr" rid="scirp.118710-ref55">55</xref>].</p><p>In our study, the fruit length and diameter of sweet pepper fruits significantly increased with the increase of harvesting time from anthesis (<xref ref-type="fig" rid="fig8">Figure 8</xref>). The minimum length and diameter of fruits (3.8 and 3.3 cm, respectively) were obtained from harvesting after week 3 of anthesis, followed by week 4 (5.2 and 4.9 cm, respectively), week 5 (9.2 and 6.0cm, respectively), and week 6 (9.5 and 6.2 cm, respectively), while the maximum length and diameter were obtained at week 7 (9.7 and 6.3 cm, respectively). In an experiment, Tadesse et al. [<xref ref-type="bibr" rid="scirp.118710-ref44">44</xref>] reported that sweet pepper fruit (cv. Domino) had attained almost 75% of its final length after three weeks of anthesis but continued to elongate at a slower rate until 10 weeks after anthesis.</p><p>Sweet pepper fruits under this experiment showed a gain in weight as the harvest time was extended (<xref ref-type="fig" rid="fig8">Figure 8</xref>). The lighter fruits (58 g) were harvested after week 3 of anthesis, which increased steadily until week 4 (87 g). After this period, fruit weight increased sharply with the extension of the harvesting time until week 5 (131 g). However, the maximum fruit weight of 150 g was obtained after week 7 of anthesis. Rahman et al. [<xref ref-type="bibr" rid="scirp.118710-ref56">56</xref>] and Tadesse et al. [<xref ref-type="bibr" rid="scirp.118710-ref44">44</xref>] reported that sweet pepper (cv. Domino) fruit fresh weight was linearly increased until eight weeks after anthesis.</p><p>Total soluble solids (TSS) are a classical tool to determine the maturity of fruits in the food industry, even though it is a destructive technique. The TSS content consists of 80% - 95% sugars, and the measure of TSS is associated with the dissolved sugars in the cell of juice [<xref ref-type="bibr" rid="scirp.118710-ref57">57</xref>]. The TSS content showed a constant increase as the fruit maturity increased, which could be seen with an increase in color fastness in the sample sweet peppers. The ascending behavior of TSS content is consistent with that reported in the literature [<xref ref-type="bibr" rid="scirp.118710-ref55">55</xref>] (<xref ref-type="fig" rid="fig9">Figure 9</xref>). The changes in TSS content of sweet pepper fruits with harvesting length. The TSS of fruit samples harvested after week 3 of anthesis was fairly low (3.0%), which was slowly increased with the increase of harvesting time. The TSS content reached a maximum level of 5.1% when fruits were harvested after week 7 of anthesis. The increase in TSS of sweet pepper fruit with maturity is probably a result of increased hexose sugar accumulation during fruit ripening [<xref ref-type="bibr" rid="scirp.118710-ref56">56</xref>], as there is a close positive correlation between the rise in TSS and soluble sugars [<xref ref-type="bibr" rid="scirp.118710-ref58">58</xref>]. The results of the present experiment are in agreement with Rahman et al. [<xref ref-type="bibr" rid="scirp.118710-ref56">56</xref>] and Tadesse et al. [<xref ref-type="bibr" rid="scirp.118710-ref44">44</xref>], who found that the TSS of sweet pepper increased slightly from 3 to 5% and 5% to 6% during the period 2 to 6 weeks after anthesis, respectively.</p><table-wrap id="table5" ><label><xref ref-type="table" rid="table5">Table 5</xref></label><caption><title> Fruit color and taste of sweet pepper fruits at different harvest stages</title></caption><table><tbody><thead><tr><th align="center" valign="middle"  rowspan="2"  >Parameter</th><th align="center" valign="middle"  colspan="5"  >Harvest stage</th></tr></thead><tr><td align="center" valign="middle" >Week 3</td><td align="center" valign="middle" >Week 4</td><td align="center" valign="middle" >Week 5</td><td align="center" valign="middle" >Week 6</td><td align="center" valign="middle" >Week 7</td></tr><tr><td align="center" valign="middle" >Fruit color</td><td align="center" valign="middle" >Green</td><td align="center" valign="middle" >Green</td><td align="center" valign="middle" >Shiny green</td><td align="center" valign="middle" >Shiny green</td><td align="center" valign="middle" >Less shiny green</td></tr><tr><td align="center" valign="middle" >Taste</td><td align="center" valign="middle" >Astringent taste</td><td align="center" valign="middle" >Astringent taste</td><td align="center" valign="middle" >Crispy pleasant flavored taste</td><td align="center" valign="middle" >Crispy pleasant flavored taste</td><td align="center" valign="middle" >Slightly flavored taste</td></tr></tbody></table></table-wrap><p>The changes in pH of sweet pepper fruits as a function of different harvesting times are shown in <xref ref-type="fig" rid="fig9">Figure 9</xref>. From the results, it was found that the pulp pH of sweet pepper fruit was not affected by different harvest maturity. The pH ranged from 5.40 to 5.70 was found during harvesting from week 3 to week 7, which was not significantly different among harvesting times. Similar results were also found (5.71% to 5.79%) by Rahman et al. [<xref ref-type="bibr" rid="scirp.118710-ref56">56</xref>], while Fox et al. [<xref ref-type="bibr" rid="scirp.118710-ref59">59</xref>] reported that sweet pepper pH was not affected by different harvesting times, which ranged from 4.9 to 5.1.</p><p>The maturity of the sweet pepper fruit was determined by a combination of different attributes. In this study, TSS, surface color change, and firmness were found to be good indicators of sweet pepper fruit maturity. Based on the results of the present study, sweet pepper fruits reached physiological maturity from week 5 to week 6. At this stage of maturity, the fruit attained 9.2 - 9.5 cm in length, 6.0 - 6.2 cm in diameter, and 131 g - 142 g in weight (<xref ref-type="fig" rid="fig8">Figure 8</xref>). Besides, fruits were shinier green, crispy, pleasant flavored, and tasty at this stage (<xref ref-type="table" rid="table5">Table 5</xref>).</p></sec></sec><sec id="s4"><title>4. Conclusion</title><p>At harvest season, determining the exact maturity of vegetable fruits is tricky. Fruits are collected at various stages of development, with some fruits being immature. As a result, even though fruits may have the same skin color at harvest, collecting fruits of different maturity at the same time is a regular concern. Immaturely chosen fruits may stay green for longer, but they may not ripen to an acceptable color and flavor, resulting in a loss of consumer confidence. Furthermore, because fruit growth continues until harvest, immature fruit will be smaller than mature fruit, resulting in a reduction in harvest yield. Tomatoes were found to be an appropriate size (6.5 cm length and 6.2 cm diameter), weight (84 g), TSS (4.5 percent), pH (4.3), “turning red” and “tasty” at the week 5 stage, while broccoli was found to be an appropriate size (12.0 cm length and 13.0 cm diameter), weight (360 g), “green” color at the day 12 stage in our study. At the week 6 stage, the nettled melon was found to be of appropriate size (15.2 cm length and 14.5 cm diameter), weight (800 g), TSS (10.8 percent), pH (6.3), “net fully developed” on the skin of the fruit, and “much tasty,” while cucumber was found to be of appropriate size (8.8 - 10.8 cm length and 2.2 - 2.9 cm diameter), weight (61 - 88 g), TSS (3.8 - 4.1 percent), pH (6.3), “less powdery with less spine” on fruit skin and “tasty” at day 10 - 12 stage. Sweet peppers that were found to be of appropriate size (9.2 - 9.5 cm length and 6.0 - 6.2 cm diameter), weight (131 - 142 g), TSS (4.5 - 4.8 percent), pH (5.6 - 5.65), “Shiny green” fruit color, and “Pleasant flavored taste” at the week 5 - 6 stage lost their attractiveness and shininess, and became slimy in texture within a short time. As a result, determining our study’s optimal maturity will help both the consumer and the grower.</p></sec><sec id="s5"><title>Acknowledgements</title><p>The project “Development of protected culture technology for safe and quality vegetables and fruit production,” financed by PIU-BARC, NATP-2, was imperative in completing this work.</p></sec><sec id="s6"><title>Conflicts of Interest</title><p>The authors declare no conflicts of interest regarding the publication of this paper.</p></sec><sec id="s7"><title>Cite this paper</title><p>Quamruzzaman, A.K.M., Islam, F., Akter, L. and Mallick, S.R. 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