The prevalence of fungi associated with seeds of some selected local flowers namely cosmos, marigold, calendula
,
and periwinkle collected from three different location
s
(Arboriculture Garden, Ramna Park Garden
,
and Horticulture Garden)
in
Dhaka district and four hybrid flower seeds (African marigold, cockscomb, petunia
,
and Portulaca) collected from Momin Beej Ghar, Dhaka district were recorded. The results showed that the highest percent germination (91.38%) of cosmos
was
obtained from Horticulture garden’s seed, marigold (95.25%) obtained from Arboriculture garden’s seed
,
and calendula (65.88%) periwinkle (79.38%) obtained from Ramna park garden’s seeds. But in
the
case of hybrid seeds, African marigold showed the highest percent seed germination (98.25%). The Horticulture garden’s seed was better than the others considering
the
incidence of fungi in all local flower seed
s
tested. But periwinkle was the lowest affected seed (12.58%,
12.20%, 8.07% incidence) and marigold was the highest affected seed (40.31%,
35.33%,
31.33% incidence) collected from Arboriculture Garden, Ramna Park
,
and Horticulture Garden, respectively. But
in
hybrid flower seeds, the lowest fungal infection (3.14%) was recorded in Portulaca and petunia seeds where
as
the highest (6.20%) was in African marigold seeds.
Flower Seed Seed-Borne Fungi Dry Inspection Blotter Agar Plate1. Introduction
Seed is the most important input for crop production. In modern agriculture, seed health is a well-recognized factor for increased production. Pathogen-free healthy seeds are considered a vital input for desired plant populations and a good harvest. Seed health testing is done to determine microbial infection or contamination for quarantine purposes. It identifies the cause of seed infection that affects the planting value of seed lots for seed certification by seed growers to supply seed to farmers. Many plant pathogens are seed-borne, which can cause enormous crop losses. Out of 16%, annual crop losses due to plant diseases, at least 10% loss is incurred due to seed-borne diseases [1]. Coincidentally important or devastating crop diseases are seed-borne and caused by fungi. It has also been demonstrated that seed-borne fungi are responsible for the poor health of seeds in many crops [2].
Flowers are important for their aesthetic and economic value. Flowers are extensively cultivated in advanced as well as in many developing countries. Floriculture plays a significant role in the economy of these countries. In Bangladesh, the cultivation of flowers is still limited. Although flowers are grown in the home gardens, community centers, schools, and office premises for beautification to a considerable extent in the city areas, its cultivation is not popular yet in rural areas. However, nowadays, commercial cultivation of flowers has increased significantly in the country. Since its inception of more than a decade, commercial floriculture in Bangladesh is about to ensure its contribution to the national economy. Now, the flower is a symbol of not only purity and beauty but also a profitable business. Many unemployed youths can enter the job market through commercial flower cultivation as the flower has a huge demand both at home and abroad. Approximately, 10,000 hectare of land is now under flower cultivation in Bangladesh. At present, there are 2000 flower shops in the country, and flowers worth Tk.100 crores are sold annually [3]. According to an article in Team India, Flower farming is nearly 5% more profitable than rice cultivation, and 2% more profitable than growing vegetables. Due to the enhanced profitability of flower production over existing crop production, farmers are showing interest in the flower industry. By exporting flowers, our country can also earn a good amount of foreign exchange. Thus, there is a great prospect for flower cultivation in Bangladesh. The common flowers grown in Bangladesh are Rose (Rosa chinensis), Tube rose (Polianthestuberose), Zinnia (Zinnia elegans), Calendula (Calendula officinalis), Marigold (Tagetesspp.), Cosmos (Cosmos bipinnatus), Balsam (Impatiens balsamina), Sunflower (Helianthus annuus), Dahlia (Dahlia sp.), Chrysanthemum (Chrysanthemum sinense), etc. Among the various flower plants, propagated by true local seed are Calendula, Marigold, Cosmos, Periwinkle and hybrid seed are African marigold, Celosia, Petunia, and Portulaca commonly cultivated in the country.
Flowers plants suffer from different diseases like other crop plants. More than 100 diseases in flower plants have been listed by the U.S. Department of Agriculture [4]. Of these, 12 diseases were caused by 20 fungal pathogens, recorded on the eight selected flower plants, Cosmos, Periwinkle, Marigold and calendula are known to be local seed-born [5]. Sultana [6] recorded five seed-borne fungi on calendula seeds and six seed-borne fungi on cosmos seeds. The prevalence of all fungi recorded varied significantly with respect to flower species and seed sources. They also found seed germination varied significantly depending on the seed sources and flower species. All these seed-borne pathogens are responsible for causing diseases in the respective flower plants mentioned and incur losses to flower production. Little work has been done on diseases of flower plants in Bangladesh. As such no literature on seed-borne diseases or seed-borne pathogens of flower plants in the country is available. Talukder [7] recorded only a few diseases on some flower plants. Consequently, there is scanty published information on seed-borne diseases or seed health of flower plants propagated by true seeds in Bangladesh. In view of the above facts, the present study has been undertaken to study germination and the prevalence of fungi in seed of some selected local and hybrid flower plants.
2. Materials and Methods2.1. Collection of Seed Samples
Seeds of eight selected flower plant species were collected from Dhaka districts. Two types of seeds were included in this study such as 1) Hybrid seeds and 2) Local seeds (Table 1). Local seeds were collected from 3 different sources viz. Arboriculture Garden, Ramna Park Garden, Horticulture Garden and hybrid seeds were collected from one source, Momin Beej Ghar, Siddique bazaar. For local seeds, four (4) seed sample were obtained from each of the three (3) seed source. Thus, 12 seed samples obtained for local seeds. For seed samples from hybrid seeds, each sample representing one seed source. Thus, altogether 16 seed samples were collected from eight selected flower plants representing 4 different seed sources; for the present study. After collection, the seeds were kept in polythene bags and brought directly to the Seed Health Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka. Seeds are stored in plastic or glass container, for both local and hybrid seeds.
Local and hybrid flower plants species included for the study
Local Name
English Name
Scientific Name
Family
Local flowers
Calendula
Calendula
Calendula officienalis
Compositae
Cosmos
Cosmos
Cosmos bipinnatus
Compositae
Ganda
Marigold
Tagetes erecta
Compositae
Periwinkle
Periwinkle
Vinca rosea
Apocynacae
Hybrid flowers
Gada
African Marigold
Tagetes erecta
Compositae
Celosia
Cockscomb
Celosia plumosa
Amaranthaceae
Petunia
Petunia
Petunia ibrida
Miscuglio
Timeful
Portulaca
Portulaca grandiflora
Portulacaceae
2.2. Inspection of Dry Seeds
In this method, only local seeds were taken for inspection. Inspection of dry local seeds was done according to the International Rules of ISTA [8]. In this method, 100 g seeds were taken from local seeds and usually inspected and graded into three categories; 1) Pure seeds 2) Seeds of other flowers 3) Inert matter. The seeds under each category were weighted and calculated the percentages of each group. After recording the data of dry inspection, rest of seeds were kept for blotter test.
2.3. Detection of Seed-Borne Fungi (Blotter Method)
Health of all the seed samples used were analyzed for detection of fungi by the Blotter Method following the International Rules for Seed Testing Association [8]. Three pieces of whatman no. 1 filter paper were soaked in sterilized water and placed at the bottom of a 9 cm plastic Petridish. The seeds were planted on the wet filter paper in the Petridish. Two hundred seeds from each sample were taken randomly and placed on the moist filter paper in 8 replicate petridishes at the rate of 25 seeds per plate. The Petridish with seeds were then incubated at 22˚C ± 2˚C under 12\12 hours alternating cycle Near Ultra-Violet (NUV) light and dark in the incubation room of the Seed Health Laboratory, SAU, Dhaka for seven days. After incubation, the plates were examined under stereo-microscope for detection of pathogens. Germination % of the seeds was also recorded. The fungi were identified up to the species following the keys of Mathur and Kongsdal [9].
2.4. Agar Plate Technique
In the agar plate method, generally surface-disinfected (0.05% Hgcl2 for 30 sec) seeds were planted on an agar medium and the plated seeds were usually incubated for 5 days at 22˚C - 25˚C under 12 h alternating cycles of light and darkness. At the end of incubation period, fungi growing out from seeds on the agar medium were examined and identified. In this method, Potato Dextrose Agar (PDA) (Potato 200 g, dextrose 20 g, Agar 15 g and H2O-1000 ml) having pH 6.5 was used. About 15 ml of the media were poured in each sterilized glass Petridish.
2.5. Statistical Analysis
The data were analysis following the Completely Randomized Design (CRD). The mean differences for efficiency of the treatments were judged by least significant difference (LSD) test.
3. Results
The results obtained from present study for dry inspection, different germination rate, seed-borne infection and other analyses have been presented and discussed in this chapter.
3.1. Dry Inspection of Local Seed
The results of dry inspection of seeds of four local flowers collected from three different locations (Arboriculture Garden, Ramna Park Garden and Horticultural Garden) of Dhaka district were presented in Table 2. It was observed that three categories of inspections viz. 1) pure seed, 2) inert matter and 3) seeds of other crops were done in respect of individual flower according to the location (Figure 1). The percent of pure seeds, inert matter and seeds of other crops respectively were measured from all the samples ranged between 90% - 98.5%, 1.5% - 5.5% and 1% - 3.5% respectively. The highest pure seeds were achieved from Ramna Park Garden for Cosmos (95.5%), Calendula (93%) and Periwinkle (96%) but from Horticultural Garden for Marigold (98.5%). In case of inert matter, the lowest percentage was obtained from Horticultural Garden for Marigold (1.5%) where the highest (5.5%) was also in Calendula from Horticultural Garden. The lowest contamination (1%) of other seeds with the targeted seeds was in Marigold collected from Horticultural Garden but the highest was observed in Calendula collected from Arboriculture Garden, Asadgate.
Dry inspection of selected local seed samples of three locations
Name of the flower plants
Name of the location
Pure seed (%)
Inert matter (%)
Seeds of other crops (%)
Cosmos
Arboriculture garden
94.50
3.00
2.50
Ramna park garden
95.50
3.00
1.50
Horticulture garden
93.00
3.50
3.50
Marigold
Arboriculture garden
97.00
2.00
1.00
Ramna park garden
96.50
2.50
1.00
Horticulture garden
97.50
1.50
1.00
Calendula
Arboriculture garden
92.00
4.50
3.50
Ramna park garden
93.00
3.50
3.50
Horticulture garden
91.00
5.50
3.50
Periwinkle
Arboriculture garden
95.50
2.50
2.00
Ramna park garden
96.00
2.00
2.00
Horticulture garden
95.00
2.50
2.50
3.2. Germination of Local Flower and Hybrid SeedsBlotter Method
1) Cosmos
Percent (%) seed germination of cosmos flower seeds collected from different location such as Arboriculture, Ramna Park and Horticultural Garden in Dhaka district varied significantly at different days after sowing (7 and 14 DAS) (Table 3). The germination percentage of this flower seeds collected from three locations was ranged between 88.60% - 91.50% at 7 DAS and 88.25% - 91.38% at 14 DAS. It was observed that the highest germination percentage; 91.50% and 91.38% respectively was in Horticultural garden’s seeds at 7 DAS and 14 DAS respectively. On the other hand, the lowest germination percentage; 88.60% and 88.25% respectively was in Arboriculture garden’s seeds at 7 DAS and 14 DAS respectively. The results obtained from Ramna park garden’s seeds showed intermediate result compared to the germination percentage of Arboriculture and Horticultural garden’s seeds.
2) Marigold
Percent (%) seed germination of marigold flower seeds collected from different location such as Arboriculture Garden, Ramna Park and Horticultural Garden in Dhaka district varied significantly at 14 DAS but there was no significant difference at 7 DAS (Table 3). The germination percentage of this flower seeds collected from three locations was ranged between 84.15% - 95.25% at 14 DAS.
Germination of local and hybrid flower seeds collected from three different locations (blotter method)
Treatments/Locations
% Seed germination
7 DAS
14 DAS
Cosmos
Arboriculture garden
88.25 c
88.60 c
Ramna park
90.00 b
90.00 b
Horticultural garden
91.38 a
91.50 a
Marigold
Arboriculture garden
95.50 a
95.50
Ramna park
88.88 b
95.38
Horticultural garden
84.15 c
96.25
Calendula
Arboriculture garden
64.75 a
74.25 b
Ramna park
65.88 a
75.88 a
Horticultural garden
57.50 b
63.63 c
Periwinkle
Arboriculture garden
77.75
84.25 b
Ramna park
79.38
86.50 a
Horticultural garden
76.50
85.63 a
Momin Beej Ghar
African marigold
96.00 a
98.25 a
Celosia
95.70 a
96.00 b
Petunia
91.25 b
91.38 c
Portulaca
95.13 ab
95.13 b
It was observed that the highest germination percentage (95.25%) was in Arboriculture garden’s seeds where the lowest (84.15%) was in Horticultural garden’s seeds at 14 DAS respectively. The results obtained from Ramna park garden’s seeds showed intermediate result compared to the germination percentage of Arboriculture and Horticultural garden’s seeds.
3) Calendula
Percent (%) seed germination of calendula flower seeds collected from different location such as Arboriculture Garden, Ramna Park and Horticultural Garden in Dhaka district varied significantly at different days after sowing (7 and 14 DAS) (Table 3). The germination percentage of this flower seeds collected from three locations was ranged between 63.63% - 75.88% at 7 DAS and 57.5% - 65.88% at 14 DAS. It was observed that at 7 DAS, the highest germination percentage (75.88%) was in Ramna park garden’s seeds where the lowest (63.63%) was in Horticultural garden’s seeds. Again, at 14 DAS the highest germination percentage (65.88%) was in Ramna park garden’s seeds which were statistically identical with Arboriculture garden’s seeds but the lowest was obtained from Horticultural garden’s seeds.
4) Periwinkle
Percent (%) seed germination of Periwinkle flower seeds collected from different location such as Arboriculture Garden, Ramna Park and Horticultural Garden in Dhaka district varied significantly at different days after sowing (7 and 14 DAS) (Table 3). The germination percentage of this flower seeds collected from three locations was ranged between 84.25% - 86.5% at 7 DAS and 76.5% - 79.38% at 14 DAS. It was observed that the highest germination percentage at 7 DAS (86.5%) was in Ramna park garden’s seeds which were statistically identical with Horticultural garden’s seeds but the lowest was obtained from Arboriculture garden’s seeds. It was also evident that at 14 DAS there was no significant effect on percentage of Periwinkle seeds germination collected from Arboriculture Garden, Ramna Park and Horticultural Garden.
5) Hybrid flower
Percent (%) seed germination of four hybrid flower seeds such as African marigold, Celosia, Petunia and Portulaca varied significantly at different days after sowing (7 and 14 DAS) (Table 3). These four hybrid flower seeds were collected from Momin Beej Ghar. The germination percentage of these four hybrid flower seeds was ranged between 91.25% - 96% at 7 DAS and 91.38% - 98.25% at 14 DAS. It was observed that the highest germination percentage (96%) was in African marigold and Celosia seeds where the lowest (91.25%) was in Petunia seeds at 7 DAS. On the other hand, at 14 DAS the germination percentage (98.25%) was in African marigold seeds but the lowest (95.13%) was in Portulaca seeds.
3.3. Seed-Borne Fungi Recorded on Local and Hybrid Flower SeedsBlotter Method
1) Cosmos
The most predominant fungus of cosmos was Alternariaalternata. The prevalence of Alternariaalternata ranged between 6.5% - 24.25% (Table 4). The incidence of individual fungi recorded on cosmos seeds varied significantly with respect to sources of seeds collection. The maximum (24.25%) incidence of Alternariaalternata was recorded in seeds collected from Arboriculture Garden, Dhaka; while the minimum (6.5%) was found at Horticultural Garden, Dhaka (Figure 2). The highest occurrence of Aspergillusflavus (3.88%) and Aspergillusniger (3.75%) were recorded at Horticulture Garden, under Dhaka district and the lowest (0.88%) and (1.75%) were founded at Ramna Park Garden and Arboriculture Garden respectively. The highest count of Fusariumsemitectum was observed at Ramna Park Garden; while the lowest incidence of the fungus (0.88%) was encountered at Horticultural Garden under Dhaka district. The maximum (2.5%) prevalence of Penicilliumsp.was recorded at Horticultural Garden; while the minimum (0.25%) was found at Ramna Park Garden. In case of Rhizopussp.,there was no significant variations were found.
Prevalence of seed-borne fungi of Cosmos seeds collected from three different sources (blotter method)
Treatments
% Seed-borne infection
Alternaria alternata
Penicillium sp.
Fusarium semitectum
A. niger
A. flavus
Rhizopus sp.
Cosmos
Arboriculture garden
24.25 a
0.38 b
2.00 b
1.75 b
1.38 b
0.88
Ramna park
17.13 b
0.25 b
6.25 a
3.25 ab
0.88 b
0.88
Horticultural garden
6.500 c
2.50 a
0.88 c
3.75 a
3.88 a
0.88
LSD (P = 0.05)
2.594
0.902
0.446
1.801
0.996
NS
CV (%)
2.22
3.43
4.42
2.51
2.44
3.76
2) Marigold
Marigold seeds were tested in blotter method (Figure 3) and the most predominant fungus of marigold within all the pathogens under the experiment was Alternariaalternata. Among the pathogens, incidence of Alternariaalternata was ranged between 35.88% - 40.97% (Table 5). The incidence of individual fungi recorded on marigold seeds varied significantly with respect to sources of seeds collection. The maximum (40.97% and 2.87% respectively) incidence of Alternariaalternata and Penicilliumsp.respectively was recorded in seeds collected from Arboriculture Garden and Ramna Park Garden respectively under Dhaka which was statistically identical with seeds collected from Horticultural Garden, Dhaka in case of the both pathogens; while the minimum (35.88% and 1.25% respectively) was found at Ramna Park Garden, Arboriculture Garden respectively. The highest occurrence of Fusariumsemitectum (2.75%), Aspergillusniger (2.50%) and Rhizopussp.(1.38%) were recorded from Arboriculture Garden’s seeds, Dhaka where the lowest incidence was found in Horticultural Garden’s seeds which was statistically identical with seeds collected from Ramna Park, Dhaka. The highest count of Aspergillusflavus (7%) was observed in Horticultural Garden’s seed; while the lowest was (2%) in Arboriculture garden’s seed, Dhaka.
Prevalence of seed-borne fungi of Marigold seeds collected from three different sources (blotter method)
Treatment
% Seed-borne infection
Alternari a alternata
Penicilliu m sp.
Fusarium semitectum
A. niger
A. flavus
Rhizopu s sp.
Marigol d
Arboriculture garden
40.97 a
1.25 b
2.75 a
2.50 a
2.00 c
1.38 a
Ramna park
35.88 b
2.88 a
1.25 b
0.88 b
4.75 b
0.88 b
Horticultural garden
40.25 a
2.50 a
1.00 b
0.88 b
7.00 a
0.88 b
LSD (P = 0.05)
2.594
0.902
1.412
1.090
1.746
0.357
CV (%)
2.14
2.53
3.87
3.22
3.35
3.99
3) Calendula
Considerable influence was caused by six pathogens under the experiment. The most predominant fungus of calendula was Fusariumsemitectum (Figure 4). The occurrence of Fusariumsemitectum ranged between 10% - 22.75% (Table 6). The incidence of individual fungi recorded on calendula seeds varied significantly with respect to sources of seeds collection. The maximum (22.75%) incidence of Fusariumsemitectum was recorded in seeds collected from Asadgate Garden, Dhaka which was statistically identical with seeds collected from Ramna Park, Dhaka; while the minimum (10%) was found at Horticultural Garden’s seed, Dhaka. Similar results were scrutinized in Ramna park garden’s seeds in case of Alternariaalternata (4.5%) and Aspergillusflavus (3.38%) and were highest occurrence but the lowest were differed between Horticultural Garden and Arboriculture garden’s seeds respectively. The fact obtained from Horticultural garden’s seeds in case of Aspergillusniger (8.25%) and Rhizopussp.(1.38%) were highest compared to Arboriculture and Ramna park garden’s seeds. But the fungal effect of Penicilliumsp.on marigold seeds collected from different location was not substantial.
Prevalence of seed-borne fungi of Calendula seeds collected from three different sources (blotter method)
Treatment
% Seed-borne infection
Alternaria alternata
Penicillium sp.
Fusarium semitectum
A. niger
A. flavus
Rhizopus sp.
Calendula
Arboriculture garden
3.50 b
0.38
22.75 a
4.38 b
0.38 c
1.00 b
Ramna park
4.50 a
0.50
21.88 a
5.38 b
3.38 a
0.63 c
Horticultural garden
1.88 c
0.88
10.00 b
8.25 a
2.25 b
1.38 a
LSD (P = 0.05)
0.746
NS
2.471
1.090
0.996
0.357
CV (%)
4.14
3.56
3.71
3.55
2.88
2.18
4) Periwinkle
Fungal effect on periwinkle seeds collected from different location of Dhaka district was literally low compared to all other flower’s seeds under the experiment (Table 7). It was evident that there was no significant effect of Alternariaalternata, Penicilliumsp.and Aspergillusniger on Periwinkle seeds collected from different location of Dhaka (Arboriculture Garden, Ramna Park and Horticultural Garden). The results obtained from Horticultural garden’s seeds in case of Fusariumsemitectum (2.88%), Aspergillusflavus (8.88%) and Rhizopussp.(1.5%) were highest occurrence compared to Arboriculture Garden and Ramna park garden’s seeds. But the lowest fungal infestation of Fusariumsemitectum (0.63%), Aspergillusflavus (7%) and Rhizopussp. (0.38%) in periwinkle seeds was found from Arboriculture garden’s seed.
5) Hybrid flower
Dominance of seed-borne fungi of some selected hybrid seeds collected from Momin Beej Ghar, Dhaka was tested on blotter (Figure 5) and six fungus infections under the experiment with the determination of pathogenic incidence by blotter method (Table 8). It is evident that the fungal infestation of Alternariaalternata and Aspergillusflavus was significant among the six pathogens. It was found that the highest incidence of Alternariaalternata (1.75%) and Aspergillusflavus (1%) was in African marigold seeds where the lowest (0.25% and 0.25% respectively) was in Portulaca seeds. The results obtained from African marigold, Celosia, Petunia and Portulaca in respect of all the pathogens under the experiment had no significant effect in case of Penicilliumsp., Fusariumsemitectum, Aspergillusniger and Rhizopussp.
Prevalence of seed-borne fungi of Periwinkle seeds collected from three different sources (blotter method)
Treatment
% Seed-borne infection
Alternaria alternata
Penicillium sp.
Fusarium semitectum
A. niger
A. flavus
Rhizopus sp.
Periwinkle
Arboriculture garden
0.38
0.75
0.63 c
1.13
7.00 c
0.38 c
Ramna park
0.75
0.75
1.63 b
1.63
7.75 b
0.75 b
Horticultural garden
0.50
1.00
2.88 a
1.38
8.88 a
1.50 a
LSD Value (P = 0.05)
NS
NS
0.884
NS
0.796
0.534
CV (%)
2.14
3.65
3.87
4.29
3.77
2.66
Prevalence of seed-borne fungi of some selected hybrid seeds collected from Momin Beej Ghar (blotter method)
Treatment
% Seed-borne infection
Alternaria alternata
Penicillium sp.
Fusarium semitectum
A. niger
A. flavus
Rhizopus sp.
Momin Beej Ghar
African marigold
1.75 a
0.63
0.25
0.50
1.00 a
0.00
Celosia
1.63 a
0.50
0.25
0.38
0.88 a
0.13
Petunia
0.88 b
0.50
0.25
0.25
0.50 b
0.13
Portulaca
0.25 c
0.25
0.25
0.25
0.25 c
0.13
LSD Value (P = 0.05)
0.826
NS
NS
NS
0.152
NS
CV (%)
3.44
4.44
2.72
5.85
4.94
5.31
3.4. Agar Plate Method3.4.1. Cosmos
The most predominant fungus of cosmos was Alternariaalternate (Figure 6). The prevalence of Alternariaalternata ranged between 15.25% - 16.75% (Table 9). The incidence of individual fungi recorded on cosmos seeds varied significantly with respect to sources of seeds collection. The maximum (16.75%) incidence of Alternariaalternata was recorded in seeds collected from Ramna park garden, Dhaka; while the minimum (15.25%) was found at Arboriculture garden’s seeds, Dhaka. The highest occurrence of Penicilliumsp.(4.5%) and Fusariumsemitectum (2.25%) were recorded at Horticulture Garden, under Dhaka district and the lowest (3.25%) and (0.5%) were found respectively at Arboriculture garden’s seed, Dhaka. On the other hand, the highest count of Aspergillusniger (7%), Aspergillusflavus (9.13%) and Rhizopussp.(2.13%) was observed at Arboriculture garden’s seed; while the lowest incidence of the fungus (4.13%), (6.63%) and (0.63%) respectively was encountered at Ramna park garden’s seeds under Dhaka district.
3.4.2. Marigold
The most predominant fungus of marigold within all the pathogens under the experiment was Fusariumsemitectum. Among the pathogens, incidence of Alternariaalternata was ranged between 18.00% - 21.63% (Table 10). The incidence of individual fungi recorded on marigold seeds varied significantly with respect to sources of seeds collection. The maximum (21.63%) incidence of Alternariaalternata was recorded in seeds collected from Ramna park garden’s seeds, Dhaka; while the minimum (18%) was found at Arboriculture garden’s seeds. Similar result was also found in case of Fusariumsemitectum where the highest (1.38%) and lowest (0.38%) respectively incidence was recorded at
Prevalence of seed-borne fungi of Cosmos seeds collected from three different sources (Agar plate method)
Treatments
% Seed-borne infection
Alternaria alternata
Penicillium sp.
Fusarium semitectum
A. niger
A. flavus
Rhizopus sp.
Cosmos
Arboriculture garden
15.25 b
3.25 c
0.50 c
7.00 a
9.13 a
2.13 a
Ramna park
16.75 a
4.00 b
1.38 b
4.13 c
6.63 b
0.63 b
Horticultural garden
16.00 ab
4.50 a
2.25 a
6.00 b
6.88 b
0.75 b
LSD (P = 0.05)
1.095
0.428
0.276
0.734
1.290
0.464
CV (%)
2.87
3.69
5.12
2.73
4.15
2.21
Prevalence of seed-borne fungi of Marigold seeds collected from three different sources (Agar plate method)
Treatment
% Seed-borne infection
Alternaria alternata
Penicillium sp.
Fusarium semitectum
A. niger
A. flavus
Rhizopus sp.
Marigold
Arboriculture garden
18.00 c
4.63 a
0.38 c
5.50 a
2.63
0.63
Ramna park
19.75 b
2.25 b
1.38 a
2.50 c
2.25
0.75
Horticultural garden
21.63 a
2.38 b
0.75 b
4.25 b
2.63
0.63
LSD (P = 0.05)
1.095
0.428
0.276
0.734
NS
NS
CV (%)
4.56
1.11
2.39
3.78
2.45
3.82
Ramna park garden and Arboriculture garden’s seeds respectively. On the other hand, the highest occurrence of Penicilliumsp. (4.63%) and Aspergillusniger (5.5%) were recorded from Arboriculture garden’s seeds, Dhaka where the lowest incidence; 2.25% and 2.5% respectively were found in Ramna park garden’s seeds, Dhaka. The result obtained from Arboriculture, Ramna Park and Horticultural garden’s seeds, Dhaka according to the incidence of Aspergillusflavus and Rhizopusspshowed statistically insignificant result.
3.4.3. Calendula
Considerable influence was caused by six pathogens under the experiment. The most predominant fungus of calendula was Fusariumsemitectum and next to Aspergillusniger. The occurrence of Fusariumsemitectum and Aspergillusniger respectively were ranged between 9.25% - 11.63% and 6.38% - 8.75% respectively (Table 11). The incidence of individual fungi recorded on calendula seeds varied significantly with respect to sources of seeds collection. The maximum; 1.25%, 11.63% and 1.75% respectively incidence of Alternariaalternata, Fusariumsemitectum and Aspergillusflavus respectively was recorded in seeds collected from Arboriculture Garden, Dhaka and the minimum incidence 0.38%, 9.25% and 0.75% respectively was recorded from Horticultural garden’s seeds, Dhaka. On the other hand, the incidence of Penicilliumsp.(2.88%), Aspergillusniger (8.75%) and Rhizopussp. (1.75%) was highest in Ramna park garden’s seeds where the lowest (0.25%, 6.38% and 0.12% respectively) was in Arboriculture garden’s seeds.
3.4.4. Periwinkle
Fungal effect on periwinkle seeds collected from different location of Dhaka district was significant under the experiment (Table 12). The most predominant fungus of Periwinkle was Fusariumsemitectum and next to Aspergillusflavus. It was evident that the highest effect of Fusariumsemitectum (6.25%) was in Ramna park garden’s seeds which was statistically identical with Horticultural garden’s seeds (6%) but the lowest (4.63%) was in Arboriculture garden’s seeds. Again, the highest occurrence of Aspergillusniger (1.88%) and Aspergillusflavus (7%) was in Arboriculture garden’s seeds but the lowest (0.38% and 4% respectively) was in Ramna park garden’s seeds. It was also observed that there was no significant effect of Alternariaalternata, Penicilliumsp.and Rhizopussp.on periwinkle seeds collected from Arboriculture Garden, Ramna Park and Horticultural Garden, Dhaka.
Prevalence of seed-borne fungi of Calendula seeds collected from three different sources (Agar plate method)
Treatment
% Seed-borne infection
Alternari a alternata
Penicilliu m sp.
Fusarium semitectu m
A. niger
A. flavus
Rhizopu s sp.
Calendul a
Arboriculture garden
1.25 a
0.38 b
11.63 a
7.25 b
1.75 a
0.25 b
Ramna park
0.63 b
2.88 a
10.13 b
8.75 a
1.25 b
1.75 a
Horticultural garden
0.38 b
0.25 b
9.250 c
6.38 c
0.75 c
0.13 b
LSD (P = 0.05)
0.413
0.2759
0.428
0.734
0.473
0.464
CV (%)
3.25
2.24
4.12
3.11
1.26
2.86
Prevalence of seed-borne fungi of Periwinkle seeds collected from three different sources (Agar plate method)
Treatment
% Seed-borne infection
Alternaria alternata
Penicillium sp.
Fusarium semitectum
A. niger
A. flavus
Rhizopus sp.
Periwinkle
Arboriculture garden
0.63
0.50
4.63 b
1.88 a
7.00 a
0.25
Ramna park
0.50
0.00
6.25 a
0.38 b
4.00 c
0.00
Horticultural garden
0.25
0.00
6.00 a
1.00 b
4.88 b
0.00
LSD Value (P = 0.05)
NS
NS
0.428
0.734
0.473
NS
CV (%)
3.73
1.34
4.85
4.05
4.61
2.78
3.4.5. Hybrid Flower Seed
Dominance of Seed-borne fungi associated with some selected hybrid seeds collected from Momin Beej Ghar, Dhaka was determined under the experiment by agar plate method (Table 13). It was evident that there was no fungal effect of Alternariaalternata, Fusariumsemitectum and Rhizopussp.in petunia and Portulaca seeds, Penicilliumsp.and Aspergillusniger in celosia seeds and Rhizopussp.in African marigold seeds. But the result revealed that the highest infestation of Alternariaalternata (3.25%), Penicilliumsp.(2.63%), Aspergillusniger (2.25%) and Aspergillusflavus (2.75%) respectively were recorded from African marigold, Portulaca, African marigold and Celosia flower’s seeds respectively. On the other hand, lowest infestation of Alternariaalternata (1.25%), Penicilliumsp.(1.75%), Aspergillusniger (0.63%) and Aspergillusflavus (0.75%) respectively were recorded from Celosia, Petunia, Portulaca and African marigold flower’s seeds respectively. It was also evident that the four hybrid flower seeds collected from Momin Beej Ghar were not significantly influenced by Fusariumsemitectum and Rhizopussp.among the six pathogens under the experiment.
3.5. Total Infection in Local Flower Seed
Total infection of fungi in different local flower seeds collected from different place in Dhaka district was significant (Table 14). It was observed that incase of pathogenic infection in Cosmos, Marigold, Calendula and Periwinkle seeds the Horticultural garden’s seed was better than the others. But according to flower’s, Periwinkle was the lowest affected seed and Marigold was the highest affected seed.
3.6. Total Infection in Hybrid Flower Seed
Significant variation was observed in case of total pathogenic infection in hybrid seeds (Table 15). It was observed that the lowest fungal infection (2.82%) was celosia and petunia seeds where the highest (5.82%) was in African marigold seeds.
Prevalence of seed-borne fungi of some selected hybrid seeds collected from Momin Beej Ghar (Agar plate method)
Treatment
% Seed-borne infection
Alternaria alternata
Penicillium sp.
Fusarium semitectum
A. niger
A. flavus
Rhizopus sp.
Momin Beej Ghar
African marigold
3.25 a
1.88 b
0.13
2.25 a
0.75 c
0.00
Celosia
1.25 b
0.00 c
0.13
0.00 d
2.75 a
0.38
Petunia
0.00 c
1.75 b
0.00
1.38 b
1.38 bc
0.00
Portulaca
0.00 c
2.63 a
0.00
0.63 c
1.63 b
0.00
LSD Value (P = 0.05)
0.565
0.677
NS
0.611
0.663
NS
CV (%)
3.43
4.30
2.94
5.64
4.29
4.00
Prevalence of percent total seed-borne infection in local flower seed collected from different locations
Treatment
% Seed-borne infection
Measured by Blotter method
Measured by agar plate method
Average
Arboriculture garden
Ramna park garden
Horticultural garden
Arboriculture garden
Ramna park garden
Horticultural garden
Arboriculture garden
Ramna park garden
Horticultural garden
Cosmos
30.64 b
28.64 c
18.39 c
37.26 a
33.52 a
36.38 a
33.95 b
31.08 b
25.33 b
Marigold
48.85 a
41.77 a
45.51 a
31.77 b
28.88 b
32.27 b
40.31 a
35.33 a
31.33 a
Calendula
32.39 b
36.27 b
24.64 b
22.51 c
25.39 c
17.14 c
27.45 c
30.83 b
18.39 c
Nayantara
10.27 c
13.26 d
16.14 c
14.89 d
11.13 d
12.13 d
12.58 d
12.20 d
8.071 d
LSD Value (P = 0.05)
4.632
4.184
3.593
3.892
3.271
3.876
3.388
3.334
4.184
CV (%)
3.362
3.111
2.292
3.674
3.542
4.141
3.582
3.452
4.124
Prevalence of percent total seed-borne infection in hybrid flower seed collected
Treatment
% Seed-borne infection
Average
Blotter method
Agar plate method
Momin Beej Ghar
African marigold
4.13 a
8.26 a
6.20 a
Celosia
3.77 b
4.51 b
4.14 b
Petunia
2.51 c
4.51 b
3.51 c
Portulaca
1.38 d
4.89 b
3.14 c
LSD Value (P = 0.05)
0.984
1.236
1.142
CV (%)
3.11
2.59
3.62
4. Discussions
The present study reveals that there were considerable effects in case of germination percentage and fungal infection occurred on seeds obtaining from dry inspection, agar plate method and blotter method.
The results obtained with dry inspection showed that the purity of different flower seeds collected from different location was acceptable because of percent purity of seeds in every case was in desired value. This was also true incasing of the presence of percent inert matter and presence of other crop seeds.
In the experiment, the germination of seeds of different local flowers collected from different location and different hybrid seeds were measured by only blotter method. Germination percentage of different local seeds and hybrid seeds differed significantly. Results showed that for local flower seeds; Cosmos collected from Horticultural Garden, Marigold collected from a sad gate garden and Calendula and Periwinkle collected from Ramna park garden represented highest germination rate compared to others. This result was occurred due to environmental factors, source of seeds, techniques of seeds growing, post-harvest operation of seed processing etc. But in case of hybrid seeds showed higher percent of germination because of generally improved techniques are used for hybrid seed processing. The results obtained by Shrotri et al. [10] determined the pathogenicity of 20 fungi in calendula seeds and resulted significant reductions in seed-borne fungi and improvements in seed germinability.
The prevalence of the total and the individual fungal infection varied with respect of flower spices and sources of seed collection. Such variation in the occurrence of seed-borne fungi has been demonstrated in a number of crops like rice, kaon, mustard, black gram, wheat, jute and chilli by different research workers [11] [12] [13].
Two methods were used for the determination of fungal infection in seeds of different local flower seeds collected from different location and in hybrid seeds. Six fungi; Alternariaalternata, Penicilliumsp., Fusariumsemitectum, Aspergillusniger, Aspergillusflavus and Rhizopussp.were tested for pathogenecity in different seeds of local and hybrid flower seeds.
Result indicated that by Blotter method, the total pathogen for Cosmos, Marigold, Calendula and Nayantar respectively ranged between 18.39% - 30.64%, 41.77% - 48.85%, 24.64 - 36.27% and 10.27% - 16.14% respectively according to different location (Asad gate garden, Ramna park garden and Horticultural Garden of Dhaka district). Again, it was also observed that fungal incidence was higher in Marigold and Periwinkle seeds compared to Cosmos and Calendula due to all three sources of seeds. The results indicated that percent of total seed-borne infection varied with different location but according to local flower seeds, Marigold was infected with highest number of pathogen and Periwinkle was the lowest. Similar phenomenon was not represented by Agar plate method. The total pathogen for Cosmos, Marigold, Calendula and Periwinkle respectively ranged between 33.52% - 37.26%, 28.88% - 32.27%, 17.14 - 25.39% and 11.13% - 14.89% respectively according to different location (Asad gate garden, Ramna park garden and Horticultural Garden of Dhaka district). But according to local flower seeds, Cosmos had the highest pathogen prevalence and Periwinkle had the lowest. It is resulted that Periwinkle showed the lowest pathogen prevalence of four local flower seeds by the both Blotter and Agar plate methods. There are many types of fungal infections occurred by different pathogen in flower seeds [5]. According to Srivastava and Gupta [14] seed-borne fungi Alternariaalternata, Alternaria zinniae, Glomerella cingulata, Cochlioblous lunata, Phoma exigua and Fusarriumsp. caused seed rot and death of flower seedlings. The findings of the present study are supported by many scientists [15] - [23].
In case of four hybrid flower seeds (African marigold, Celosia, Petunia and Portulaca), the presence of six pathogens (Alternariaalternata, Penicilliumsp., Fusariumsemitectum, Aspergillusniger, Aspergillusflavus and Rhizopussp.) were comparatively very much low. But among the four hybrid flower seeds, African marigold showed the highest incidence of fungi where Portulaca showed the lowest. These four hybrid flower seeds were collected from one location but pathogenicity varied due to different types.
5. Conclusion
From the result obtained in the experiment, it can be concluded that in case of pathogenic infection in Cosmos, Marigold, Calendula, and Periwinkle seeds, the Horticultural garden’s seed was better than the others. But according to flowers, Periwinkle was the lowest affected seed and Marigold was the highest affected seed. But in the case of hybrid flower seeds, the lowest fungal infection was in Portulaca seeds whereas the highest was in African marigold seeds. As the study was limited to one district at four locations only, further studies with more representative seed samples from different flower species, obtained from different parts of the country should be undertaken in order to portray the exact picture regarding the prevalence of fungi, especially the pathogenic ones in flower seeds.
Acknowledgements
We thank anonymous reviewers for their kind review of the manuscript. This research was financed by the general fund of the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
Authors’ Contributions
This work was carried out in collaboration among all authors. Author MK conducted the research work. Author FMA designed and supervised the study and edited the manuscript. Author NA and MHA managed the literature searches. All authors read and approved the final manuscript.
Conflicts of Interest
The authors declare no conflicts of interest regarding the publication of this paper.
Cite this paper
Kumkum, M., Aminuzzaman, F.M., Akhtar, N. and Ali, M.H. (2022) Prevalence of Seed-Borne Fungi Associated with Seeds of Some Selected Flowers. American Journal of Plant Sciences, 13, 778-796. https://doi.org/10.4236/ajps.2022.136053
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