Fish is any food extracted from oceanic or continental waters intended primarily for human or animal consumption [1]. The fish destined for human consumption is obtained through aquaculture and fishing activities, with an annual per capita consumption of 20.5 kg worldwide [2].

Tilapia is one of the fish intended for human consumption. Tilapias are freshwater fish native to Africa, belonging to the Cichlidae family, classified into three genera: Tilapia, Oreochromis and Sarotherodon, differentiated according to parental care patterns. Species of the genus Oreochromis (O. niloticus, O. aureus, O. mossambicus) and their interspecific hybrids (red tilapia) are considered optimal culture organisms as they tolerate high densities, are fast-growing, resistant to diseases, adaptable to captivity, in addition to its meat is of high quality and affordable price [3]. Thus, tilapia for several years has become a product of the highest generation in aquaculture, for example, only in 2018, and within the group of finfish, it represented 10.2% of the total world aquaculture production, being the main providers Asian countries such as China, India, Indonesia, among others [2], and is commercialized in the market internationally mainly as a complete product or fillet [3].

Fish is considered a highly nutritious food and a fundamental part of human nutrition as it is the main source of proteins of high biological value and digestibility, vitamins, minerals, and unsaturated lipids [1] [4]. Fish and its meat, in addition to being a source of quality protein, for developing regions of the world represent an important proportion of animal protein in their diet, contribute to national economies, health, food security and improve livelihoods primarily of artisanal fishermen [5].

However, due to these nutritional properties, fish is a highly susceptible food to deterioration, loss of quality and health risk due to contamination with pathogenic bacteria throughout the food chain; the foregoing mainly because it has a pH close to neutrality, high water activity and content of nutrients easily usable by microorganisms, as well as the activity of enzymes and oxidation reactions present in fish tissues and viscera [1] [4] [6] [7].

In the fishing and aquaculture industry, the term “quality” may have different meanings, commonly associated with species of high commercial value or proportion of the piece, absence of pathogenic microorganisms or chemical compounds, with its state of freshness and appearance related to its deterioration derived from autolysis, oxidation and/or contaminating microbial activity [8].

In the period that elapses from the capture to processing and marketing, the fish can experience various changes in its sensory, physical-chemical and microbiological properties that affect its freshness, degree of conservation and shelf life, which are related to factors such as species, size, shape, fat content, feeding, capture methods, their environment, cooling conditions, storage, transportation, distribution, among others [1] [4] [6] [9].

Conservation techniques for fish or products are varied and are necessary to keep them in good condition until consumption, helping to reduce the process of deterioration or decomposition that causes the detriment of its quality. Thus, the preservation methods in foods, including fish, are applied to extend their nutritional, sensory and safety characteristics for a longer consumption time. They are based on altering the optimal conditions of biological activity (enzymatic and microbial) to water elimination, acidification and changes in salt concentrations, changes in temperatures (refrigeration, freezing or cooking), among others [5] [10]. The fillet is the edible portion of the fish most in demand by consumers [11], being in its fresh refrigerated state one of the common presentations for marketing and consumption. The fresh chilled fillet is a food product defined as the muscular portion of the fresh, healthy, clean fish, free of belly, with or without skin and/or scales, according to its presentation, obtained by cutting parallel to the spine and subjected to refrigeration where the product must be conveniently free of torn ends, tears, fins, severely discolored meat and blood clots [12]. Therefore, the objective of this study is to analyze the degree of quality of tilapia fillet (Oreochromis niloticus) subjected to conservation by refrigeration at 4˚C for twelve days.

Freshness is an attribute that varies continuously and means the fish has properties like those owned in life or after being caught or harvested. Among the main methods of evaluating the freshness or deterioration of fish, the sensory, physical, chemical, and microbiological stand out [4] [17]. Sensory evaluation is the most used method, due to its low cost, efficiency, and practicality, being commonly carried out in the fishing sector and sanitary inspection services [1].

Through sensory analysis, it is possible to determine the degree of quality of tilapia fillet for this the Mexican standards [12] and [16] describe how to carry out said procedure where the evaluation system that includes the factors in the fillet to be evaluated, and corresponding score to determine the degree of quality. The fishery products destined for human consumption by the aforementioned standards, classify the evaluated fish fillet in the three quality grades: first quality, which is when the sum of deductions assigned to the sensory characteristics ranges from 90 - 100 points, second quality of 85 - 89.5 points and out of classification < 85 points. Any fillet with a rating of less than 85 points is considered in a state of alteration and does not comply with the classification of the indicated standards.

Figure 4 presents the results of the sensory analysis of ten untrained evaluator panelists based on a point deduction system for different sensory factors of tilapia fillet (Table 1). It was determined that from day 0 to 4 the tilapia fillet had a first quality classification obtaining 98 points, on day 6 it obtained the second quality classification with 85 points and finally from day 8 it showed a category of out of classification with 53 points, points and later reaching 45 points on day 12 of refrigerated storage. The fillets from day 8 of storage presented low scores, is therefore not recommended for human consumption (Figure 4).

Figure 5 establishes the relationship between the number of days of storage elapsed and the average score from the evaluation scheme. Therefore, the higher the score obtained, the fresher the fish fillet will be, along with better quality, and the lower the score and the longer the storage time, the lower the quality and freshness. It is important to point out that the period of storage, quality and freshness, will depend on the storage conditions, where factors such as pre and postmortem handling of the fish, the speed of cooling after capture, maintenance of the cold chain, fishing gear, time of the year, fishing area or cultivation, among

others, may affect the quality of the fish [18] (Figure 6). Generic sensory evaluation methods to determine the degree of quality of fishery products such as those used in this study are useful. However, they may give rise to different opinions or judgments by not considering differences or characteristics between species of fish or products to be evaluated.

In related studies on sensory evaluation of fish fillets, Santaella et al. [19] reported that sea bream fillets (Sparus aurata L.), stored in ice-based refrigeration systems, presented sensory evaluations, outlining that after fifteen days of storage they reached rejection levels for a total storage period of twenty-two days. Meanwhile, Domínguez and Saibene [20] indicated, through sensory evaluation as well, that for shad fillet (Prochilodus lineatus) preserved in flake ice in a chamber from 0˚C to 3˚C, it had a shelf life of six days.

Tome et al. [21] in a study for tilapia (Oreochromis spp) evaluated for twenty-one days in refrigeration at 0˚C, reported through sensory evaluation a state of alteration beginning at the 10^th day of storage. Pinnacchio [22] carried out the sensory evaluation of several species of refrigerated fish (0˚C to 3˚C) such as Leporinus obtusidens, Salminus brasiliensis, Luciopimelodus patl and Prochilodus lineatus, indicating that, at the 10^th day, all the specimens studied were in a putrefaction state.

Refrigeration is a short-term food preservation method commonly used due to its ease, effectiveness, and low cost based on lowering the temperature (between 0˚C and 5˚C), slowing down enzymatic activity and developing microorganisms, extending their life useful, nutritional, and sensory properties of commercial quality [23] [24] [25]. However, refrigeration favors humidity and microbial growth, which is why it is necessary to monitor the expiration and storage time of foods under these conditions [25]. At a temperature of 4˚C, the shelf life is correlated with its pH, chemical composition, and microbiological contamination, being kept refrigerated for only a few days [24].

In fish, postmortem changes such as biochemical, physical, and microbiological are responsible for the loss of freshness and deterioration of its quality. These changes are found depending on the type of species, handling, storage, and conservation conditions [19] [26].

Fish and products, in accordance with storage conditions, are a favorable medium for microbial growth. In refrigeration this growth is of aerobic, Gram-negative bacteria and represents the main cause of deterioration in products causing some changes such as discoloration, gas production, and pH changes, among others [27].

Along with the sensory changes, aroma and flavor are the most frequent characteristics associated with the quality of the fish. The bad smell of fish is derived mainly from the microbial action of non-protein nitrogen compounds such as peptides, amino acids, nucleotides, volatile bases, and the reduction from trimethylamine oxide to trimethylamine; Although freshwater fish contain low amounts of trimethylamine oxide, unpleasant odors come from carbonyls and alcohols from polyunsaturated fatty acids [19] [26].

It has been reported that the deterioration of fish and its meat presents four phases which are: phase I, due to autolytic changes, caused by enzymes, freshly killed fish has a sweet, marine, delicate flavor. The deterioration is scarce, with a slight decrease in the characteristic aroma and flavor. Phase II, autolytic changes, caused by enzyme with significant reduction of the natural taste and smell of the fish. The meat acquires a neutral yet non-unpleasant flavor and a pleasant texture. Phase III, bacteriological changes, where signs of deterioration begin to show, there is the presence of strong unpleasant flavors, rancid and unpleasant odors; there are also significant changes in texture; the meat becomes soft and watery, or leathery and dry. And phase IV, with even more bacteriological changes, where the fish is spoiled, rotten and inedible [9].

The signs of deterioration in fish are commonly perceived through the sense organs, called sensory changes, and can be noticed at the level of the producers themselves, industrialists, health inspectors, handlers, and final consumers [19] [28]. Therefore, sensory evaluation is a tool to evaluate and determine the deterioration and useful life of fish, due to the relative ease, speed and low cost of being carried out, greatly influencing the acquisition and purchase of these foods of aquatic origin [19] [28].

The term quality in fish and products can have different meanings, focusing on appearance and freshness, degree of deterioration or absence of dangers to the health of the consumer such as bacteria, viruses, parasites or chemical compounds. So the quality can be something different for each person. It is generally thought that high-quality fish is that which is consumed within the first few hours post-mortem, but it has been reported that very fresh fish in rigor mortis is difficult to subject to filleting and skinning actions. Therefore, for the processor, slightly older fish subjected to rigor mortis is more recommended [4].

The evaluation of the quality of the fish can be carried out by chemical, physical, microbiological and sensory methods. The evaluation of sensory characteristics to determine the quality of fresh fish analyzes and interprets the characteristics of the food through the human senses of sight, smell, taste, touch, and hearing [4]. Since the consumer is considered the ultimate judge of fish quality, the use of chemicals or any other methods must be correlated with sensory evaluation. In addition, sensory evaluations must be carried out under controlled conditions to reduce variations and their effects such as the environment or personal prejudices of evaluators [4].

Different methods of sensory evaluation of fresh fish intended for human consumption have been developed in various countries around the world, either generically or for specific species (Cod, Haddock and Sole among others) in order to determine its quality and freshness [29]. One of these methods is the quality index used in Europe based on the significant sensory parameters of raw fish and a scoring system for characteristics in eyes, gills, skin, odor and hardness, among others, classifying the products evaluated into three quality levels [4] [29]. While in countries such as Mexico, generic evaluation methods or standards have been developed for fish and products such as “fillets”, also based on a scoring system for characteristics such as odor, color and texture, among others, classifying final products in three levels as are the standards used in this study.

Fish is a food of low stability and short shelf life due to intrinsic characteristics, such as its pH close to neutrality, high moisture content and water activity that favors deterioration due to microbial activity, autolysis, and oxidation.

Until the 4^th day, the tilapia fillet refrigerated at 4˚C for twelve days reached a first quality classification for its acceptance for consumption. Meanwhile, on the 6^th day, it reached the classification of second quality and finally, on the 8^th day it acquired an out-of-classification quality level due to negative sensory evaluations and food rejection.

The decrease in temperature to preserve fish and products is widely known and used, having great importance worldwide for the commercialization and availability of fresh and quality food. Refrigeration is a preservation method by reducing temperature. However, it has a short-term conservation effect through the reduction of temperature that slows down the microbial and enzymatic activity responsible for deterioration. Therefore, to preserve fish for a longer time, the combined use of refrigeration with other methods that exert an inhibitory effect on the main factors associated with deterioration, such as microbial and enzymatic activity and oxidation, is recommended.

To the Technological University of Nayarit in its engineering division in bio-food technologies for providing facilities, equipment, and materials to carry out this work. As well as to the Nayarit Unit of the Biological Research Center of the Northwest for the fish supplied for the study.

All authors contributed equally to the development of the research and manuscript.

The authors declare no conflict of interest in the development and publication of this manuscript.

Prado-Toledo, I.Y., Ramos-Santoyo, K.N., Guzmán-Robles, M.L. and Cortés-Sánchez, A. De J. (2022) Analysis of the Degree of Quality of Fish Fillet in Refrigeration. Open Journal of Applied Sciences, 12, 744-756. https://doi.org/10.4236/ojapps.2022.125050