Factors such as race, geographical locations, sex, altitude, climate, diet, and environment influence reference ranges of biochemical values [1] [2] [3]. These ranges are used in making critical decisions on laboratory results for both diagnosis and determination of therapeutic outcomes. Levels of biochemical values may be indicators of functional disorders of various body systems and organs. For example, liver function state can be assessed by measuring serum levels of: alanine aminotransaminase (ALT), aspartate transaminase (AST), gamma-glutamyl transaminase (GGT), alkaline phosphatase (ALP), total bilirubin (T.Bil), direct bilirubin (D.Bil), total protein (TP) and albumin (ALB) among others. Conversely, kidney function can be assessed by determining serum levels of creatinine (CRT) and urea [4]. Results of these assays should be compared with the reference ranges of the respective parameters in order to ascertain whether the functions of these organs are deranged or not. Incidentally, these ranges are not universally the same. For example, people of African descent have been shown to have higher ranges in ALT, AST, ALP, TP, ALB, and CRT than the Caucasians [5]. There are significantly higher median values in ALT, GGT, TP, ALB in males below 60 years than those above 60 years, but significantly lower values in T.Bil, D.Bill. Females below 60 years have demonstrated lower AST, ALT, GGT, ALP and TP median values but higher ALB, T.Bil and D.Bil than those above 60 years [6]. Significantly higher median values in males than in females have been demonstrated in: AST, ALT, ALP, GGT, T.Bil, D.Bil, Urea and CRT [7]. There is seasonal variation in biochemical values in which significant decreases in AST, ALT and T.Bil, median values between dry- and rainy-seasons among Guneans aged between 6 and 45 years have been demonstrated. In the same study, gender-based disparities were demonstrated in different age groups with males demonstrating significantly higher AST, ALT and CRT median values [8]. With the background of such variations, the recommendation for national and regional based biochemical reference intervals is justified [9]. Further to this pursuit, the current study aims at determining reference intervals for routinely evaluated biochemical values that can be used for diagnosis and research in the settings of the study.

The enrollees were majorly in business and held tertially level of education as shown in Table 1.

Males demonstrated significantly higher median values than females in AST

F = Frequency.

(26 u/l vs 24 u/l, p = 0.001), ALT (18 u/l vs 13 u/l, p < 0.001), ALP (99 u/l vs 84 u/l, p < 0.001), T.Bil (7.01 μmoles/l vs 5.49 μmoles/l, p = 0.004), D.Bil (3.42 μmoles/l vs 2.5 μmoles/l, p < 0.001), ALB (45 g/l vs 41 g/l, p < 0.001) and CRT (97 mmoles/l vs 86 mmoles/l. p = 0.001). Females demonstrated significantly higher TP median values than males (77 g/l vs 69 g/l, p < 0.001). This is shown in Table 2.

Significantly higher median values in males than in females demonstrated in AST, ALT, ALP, T.Bil, D.Bil and ALB and CRT in the current study have been reported also by Kibaya et al. (2008) among 1020 males and 521 females from different ethnic people groups residing in Kericho situated 2042 m above sea level and lying 260 km northwest of Nairobi. The results of UREA were however, not comparable in the two studies as gender-based significant difference was only demonstrated in the current study [11]. These findings clearly show that the observed gender-based differences in biochemical parameters were not tagged to the studied sample sizes or altitude. Sex hormones play major roles in the various aspects of reproduction, differentiation, growth and homeostasis in influencing development of specific gender-traits and consequently, influencing the regulation of structure and function of nearly all tissues and organs [12]. Thus their role in the observed differences in the biochemical parameters may not be exonerated. Specifically, estrogen has been reported to have protective role against cardiovascular diseases in premenopausal females when compared to age-matched males, and to postmenopause females [13]. Moreover, there are reported increases in urea, Lactate dehydrogenase and ALP values in Caucasian females above 48 years which is considered as menopausal transition period [14] [15] when levels of estrogen start declining compared with the values of those below 45 years [16]. This observation supports the role of sex hormones in influencing the levels of biochemical parameters. Contradicting reports of insignificant differences between males and females in ALT, AST ALP and TP values

Comparison of male and female parameters using Independent t-test p < 0.05 considered significant. Bold denotes significant. AST= Aspartate transaminase, ALT = alanine aminotransaminase, ALP = alkaline phosphatase, T.Bil = total bilirubin, D.Bil = direct bilirubin, ALB = albumin, CRT = creatinine. TP = total protein.

from a study conducted among 35 Indian females and 35 males [12] raises a question on the need of using large sample sizes when determining reference intervals of a population [17]. Significantly lower ALB values in females have been reported elsewhere and have been attributed to increased degradation [12]. The significantly higher TP values in females than males observed in the current study concurs with the findings observed in elderly subjects aged between 60 and 75 years [18]. Nonetheless, there is a contradictory report of insignificant gender-based differences in TP levels [5]. Although there is no obvious explanation of the reported discrepancies in TP values, it would be helpful to evaluate the performance of different assay techniques using different age group subjects and from different racial backgrounds.

In health males demonstrate significantly higher biochemical median values than females and the main possible cause of the disparities is the effect in sex hormones. It is also apparent that gender-based disparities were not tagged to the studied sample sizes or altitude. Notwithstanding too low sample sizes for example that of 35 participants did not demonstrate the significant gender-based disparities in ALT, AST ALP and TP observed in larger sample sizes.

Localized gender-based reference intervals for ALT, AST, ALP, T.Bil, D.Bil, TP, ALB, and CRT for different age groups are established for use in clinical practice and research. The reference ranges established in the current study could be used in the interpretation of laboratory test results in the setting of the study if no other appropriate reference intervals are available.

The author has no conflict of interest tagged to this study work.

Mrama, J.I. (2022) Selected Biochemical Values of Suitable Blood Donors at Kenyatta National Hospital. Open Access Library Journal, 9: e8611. https://doi.org/10.4236/oalib.1108611