Helminth is derived from the Greek word helminths—meaning, worm. Helminth is a broad categorical term referring to various types of parasitic worms that reside in the body [1]. The World Health Organization reveals that over two billion people are suffering from parasitic worm infections [2]. It is estimated that by the year 2025, about 57% of the population in developing countries will be influenced [3]. Helmintic infections are very common in men. Helminthic infections can serve as a threat to human beings, especially in developing countries. It leads to malnutrition, anemia and pneumonia. Majority of the infections, which are due to worms are mostly limited to tropical regions. Anthelmintics are drugs that may act locally to expel worms from the GIT or systemically to eradicate adult helminths or development forms that invade organs and tissues [4].

Celosia is a small genus of edible and ornamental plants belonging to the family Amaranthaceae. Among the different species in the genus celosia, C. laxa (Plate 1) is an important tropical leafy vegetable crop of high nutritional value [5]. An Indian origin of C. laxa, of tropical origin is known for its very brilliant colors with a wide range of traditional uses such as treatment of mouth sores, blood diseases, diabetes mellitus and as an aphrodisiac; with also claims of cure against ovarian and uterine diseases [6] [7]. However, C. laxa is largely a west African plant species used traditionally as an anthelmintic, anticancer, antibacterial and analgesic agent. The whole plant has also been reported for use in the treatment of diarrhea, piles, bleeding nose, as a disinfectant, inflammation, haematological and gynaecologic disorders [8].

Locally, the Hausas call it “bokan gida” or “nànnáfaá”, or “nànnàhoó”, while in Southwestern Nigeria it is called àjẹ fáwo. In west Africa of Sierra leone and Gambia, it is called “gimbui” and “furayŋamo” respectively. The study was thus prompted by the fact that there was a seeming prevalence of worm infection in Gwandu community of Gwandu Local Government Area of Kebbi State-Nigeria.

Earlier interviews among the locals in the various village communities of Gwandu LGA in Kebbi State-Nigeria, revealed several cases and complains of stomach ache and parasitic diseases leading to the use of a decocted plant species of C. laxa to effect cure. Thus, the seeming prevalence of worm infection and diseases that bore similarities with the later in these communities prompted this study. Moreover, it has been reported that most of the existing anthelmintics produce side effects such as abdominal pain, loss of appetite, nausea, vomiting, headache and diarrhea [9].

Hence, Anthelmintics from the natural sources may play a key role in the treatment of these parasite infections [10]. Literature has also shown that increasing problems of development of resistance in helminths against anthelmintics have led to the proposal of screening medicinal plants for their anthelmintic activity [11]. Consequently, based largely on folklore therapeutic claims, the present study was carried out to assess the anthelmintic activity of Celosia laxa against Pheretima posthuma using three extracts obtained from solvents of gradient polarity.

Phytochemical screening of the extracts revealed richly, the presence of alkaloids, cardiac glycosides and less richly some other metabolites which are mainly polyphenolics. These are shown in Table 1. While, chemo-microscopical studies carried out on the powdered drug of C. laxa revealed the presence of fat and oil, calcium oxalate and starch as shown in Table 2.

All anthelmintics essentially kill worms by either starving them to death or paralyzing them because worms have no means of storing energy, they must eat almost continuously to meet their metabolic needs [17]. Any disruption in this process results in energy depletion. Interfering with feeding for 24 hours or less is sufficient to kill most adult parasites. Parasites will also die if they become paralyzed and temporarily lose their ability to maintain their position in the gut [18]. Preliminary phytochemical screening of C. laxa extract recorded on Table 1 revealed the presence of proteins, saponins, steroids, carbohydrates, alkaloids, tannins, glycosides, flavonoids and phenols. Phytochemical qualitative test performed from earlier study of same plant conformed with this result [19].

Phenolic anthelmintics interfere with the energy generation in the helminth parasites by uncoupling the oxidative phosphorylation. Another possible mechanism of action is that they bind to free proteins in the gastrointestinal tract of the host animal or to glycoprotein on the cuticle of the parasite and causes death.

Key: + = detected; ++ = highly detected; -- = not detected

The possible mechanism of action of tannins may be three-fold as follows: 1) interfere with energy generation by uncoupling oxidative phosphorylation; 2) they may interfere with glycoprotein of cell surface and; 3) they can bind to free proteins in the gastrointestinal tract of host animal or glycoprotein on the cuticle of the parasite and cause death [20] [21].

Alkaloids may act on central nervous system and caused paralysis of the earthworm [18] [22]. The effect would be due to presence of the steroidal alkaloid, oligoglycosides, which may suppress the transfer of sucrose from the stomach to the small intestine; hence, couple with its antioxidant effect, it is capable of reducing the nitrate generation which could interfere in local homeostasis that

is essential for the development of helminths [23].

Praziquantel works by causing severe spasm and paralysis of the worms’ muscles, accompanied by Ca²⁺ influx inside the schistosome. Morphology alteration and a host of other factors are the mode of actions of the standard drug used [24]. Thus, the ergastic cell content recorded in the chemo-microscopy of C. laxa (Table 2), may play some role in the anthelmintic potency observed by the plant’s hydroalcoholic extracts.

Analyzing results given in Table 3 and the graphs (Figure 1 and Figure 2) showed that the aqueous extract, AE at 80 mg/ml gives higher activity (no significant, p > 0.05) compared to the standard Praziquantel, while at 10 - 40 mg/ml, there was significant activity in a dose dependant manner (with p < 0.05) compared to the standard drug. Hence, this shows that the aqueous extract, AE, as seen in the graph (Figure 1), exhibited the most anthelmintic activity with the least time required to paralyzed and completely kill the earthworms at every tested concentration. Anthelmintic potency of the aqueous extract was closely followed by the methanolic extract, ME, as shown in the graph (Figure 2), which also exhibited significant anthelmintic activity in a dose dependent manner. However, in a conversely revealed activity pattern of AE and ME, the

Values are expressed as mean ± SEM. Values were found out by using ONE way ANOVA followed by Dunnett’s t-test. ** Values are significantly different from control at (P < 0.05).

hexane extract, HE, showed no significant activity, as seen from the graph (Figure 3). These are not far-fetched, as most research groups have estimated that greater amounts of polyphenolics are resident in polar hydroalcoholic domains.

The overall findings of the present study have shown that C. laxa contains possible anthelmintic compounds due to the bioactivity records of aqueous and methanol extracts. Extract from hexane solvent had recorded no activity at the concentrations tested, indicating a likely non-synergistic mode of action by the

Plate 1. Celosia laxa thriving gracefully in cultivated land habitat (Image retrieved [16] ).

phytoconstituents in the three extracts. Alkaloids and cardiac glycosides were detected richly in the plant and hence, not ruling out their role in the bioactivity recorded. The in vitro method used has thus, provided a means of rapidly screening the plant’s extracts for the validation of its claims as an anthelmintic and hence, indicate the plant’s potentials. Further studies, using the results of these findings as a lead, might just rightly translate to the discovery of more potent anti-infectives against parasitic (helminth) worm disease.

The Lab. Technologist, Mal. Abdullahi of Pharmacology & Toxicology Department, Faculty of Pharmaceutical Sciences, UDUS, was of good technical assistance to this study.

The authors declare no conflicts of interest regarding the publication of this paper.

Mathias, S.N., Mshelia, E.H., Danbala, B.B. and Biambo, A.A. (2021) In Vitro Anthelmintic Activity of Leaf Extracts of Celosia laxa Schum. & Thonn. Open Journal of Applied Sciences, 11, 1277-1286. https://doi.org/10.4236/ojapps.2021.1112097